Rorug03G0175900

disease resistance RPP13-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001713
Physical Location & Seq
Reverse (-)
14898697 .. 14901319
2623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0175900.1

Sequence Viewer

Length: 1272 bp
ATGAGAACCAAAACAACATTCTTCTTTTGGCTCTCTGCGTTGGCTATTGCACTAGTTTCCATTACCTTTGCACTCCGCAGCTTCTCTTCTCTTTCCTCAACTTCTGTAATCTCCAACTTTGTCCAAACCGGCTCCGATCTTGCCATGTTTGATGTTTTTTTCAAACAAAGTGTTGAGACCTTATTGTCAACCGTGAGAAGCATTGTGTCCAGACACCATCATCACCAGCAGAGGATAAACTGCGACGTGGAAAAATGGAACTCGTGGCTCATATCCGAATTCAATGTGTCACTTGTTTTGACTGTTGGCTTAAATGGCTGCACAAACTTTAGTAGTGTGCAAAAGGCAGTAGATGCCGCTCCTGAGCTTAGCACTAATAGGACACTCATCATAATCGACTCAGGTACTTACAGGGAGAAGGTGACAGTTAATGTTAGCAAGACCAATTTGATATTTCAAGGTCAGGGTTATCTTAACACTGCCATAGCTTGGAATGACTCGGCCAATTCTTCTGGGGGTACTGGCCGAAGCTCTTCAGTTTCTATATCTGCTCCCAATTTCACTGCCTACAACATTAGCTTTAAGAACACAGCTCCGCCACCATCAAAAGGCAGAATTGGAGCACAGGCAGTGGCACTTCGAGTTTCTGGAGATCAAGCAGCATTTTATGGATGTGGTTTCTATGGGGCGCAAGACACTCTCAATGACGACCGTGGAAGACATTATTTTAGAGAATGTTTCATTCAAGGATCCATAGATTTCATTTTCGGAAGCGCTAGATCACTATATGAGGGGTGCACCATTAATTCAATAGCAAATCCAGTGAAAGGTGGGATTCCACTGAAAGGTAGGATAAGTGGAGCCATCACAGCACAAGGAAGGCAATCGATGCAGGAGCAAAGTGGGTATTCCTTTGTGAATTGCAGTATTGGAGGAAGTGGGAGAGTGTGGCTTGGTCGAGCTTGGGGAGCTTACGCCACTGTGGTGTTCTCCAAAACGTACATGTCTGACGTTGTTGCTTCAGATGGTTGGAATGACTGGAGTGACCCTTCTAGAGATCAGAAAGTGTTTTATGGGGAGTATGAGTGCATAGGACCAGGAGCAAATAATACAAATAGGGTCTCATATTCAAAGCAGCTCAACGAATCTGAAGCAGCACCCTTTTTGGATATTTCCTACATTGATGGGGATGAATGGCTTTTATATGCTCCACTCTTCAGTCTTCATGAAGATTACCATATGCACAATTATCAGTTTACTTATACTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

423

Amino Acids

46.61

Weight (kDa)

6.71

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 101 - 397 1.6e-66 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 184
AccB7I CCANNNNNTGG 1 cut(s) 489
AccBSI CCGCTC 1 cut(s) 359
AciI CCGC 3 cut(s) 76, 357, 596
AclWI GGATC 2 cut(s) 744, 757
AcoI YGGCCR 2 cut(s) 501, 523
AcsI RAATTY 1 cut(s) 278
AcuI CTGAAG 4 cut(s) 519, 1005, 1170, 1201
AfaI GTAC 3 cut(s) 406, 520, 1001
AfeI AGCGCT 1 cut(s) 775
AfiI CCNNNNNNNGG 4 cut(s) 489, 608, 827, 845
AflIII ACRYGT 1 cut(s) 1002
AgsI TTSAA 6 cut(s) 163, 283, 458, 746, 810, 1131
AhlI ACTAGT 1 cut(s) 52
AjiI CACGTC 1 cut(s) 247
AjnI CCWGG 1 cut(s) 1096
AjuI GAANNNNNNNTTGG 1 cut(s) 34
AleI CACNNNNGTG 1 cut(s) 983
AluBI AGCT 9 cut(s) 81, 367, 488, 531, 579, 593, 962, 971, 1138
AluI AGCT 9 cut(s) 81, 367, 488, 531, 579, 593, 962, 971, 1138
Alw21I GWGCWC 2 cut(s) 625, 800
Alw26I GTCTC 2 cut(s) 170, 1126
Alw44I GTGCAC 1 cut(s) 796
AlwI GGATC 2 cut(s) 744, 757
Aor51HI AGCGCT 1 cut(s) 775
AoxI GGCC 2 cut(s) 501, 523
ApaLI GTGCAC 1 cut(s) 796
ApeKI GCWGC 5 cut(s) 78, 318, 659, 1135, 1154
ApoI RAATTY 1 cut(s) 278
AseI ATTAAT 1 cut(s) 804
Asp700I GAANNNNTTC 1 cut(s) 532
AspLEI GCGC 2 cut(s) 691, 776
AspS9I GGNCC 1 cut(s) 1094
AsuHPI GGTGA 2 cut(s) 215, 433
AvaII GGWCC 1 cut(s) 1094
BaeGI GKGCMC 1 cut(s) 800
BamHI GGATCC 1 cut(s) 749
BauI CACGAG 1 cut(s) 262
BbsI GAAGAC 2 cut(s) 724, 1214
Bbv12I GWGCWC 2 cut(s) 625, 800
BbvI GCAGC 5 cut(s) 90, 305, 671, 1147, 1166
BccI CCATC 5 cut(s) 225, 610, 872, 1019, 1178
BciT130I CCWGG 1 cut(s) 1098
BcoDI GTCTC 2 cut(s) 170, 1126
BcuI ACTAGT 1 cut(s) 52
BfaI CTAG 4 cut(s) 53, 777, 1053, 1270
BfoI RGCGCY 1 cut(s) 777
BisI GCNGC 6 cut(s) 79, 319, 357, 660, 1136, 1155
BlpI GCTNAGC 1 cut(s) 368
BlsI GCNGC 6 cut(s) 80, 320, 358, 661, 1137, 1156
Bme1390I CCNGG 1 cut(s) 1098
Bme18I GGWCC 1 cut(s) 1094
BmgBI CACGTC 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 1094
BmiI GGNNCC 3 cut(s) 133, 751, 862
BmrFI CCNGG 1 cut(s) 1098
BmsI GCATC 2 cut(s) 343, 879
BpiI GAAGAC 2 cut(s) 724, 1214
BpmI CTGGAG 2 cut(s) 669, 1060
Bpu10I CCTNAGC 1 cut(s) 363
Bpu1102I GCTNAGC 1 cut(s) 368
Bsa29I ATCGAT 1 cut(s) 887
BsaI GGTCTC 2 cut(s) 170, 1126
BsaJI CCNNGG 1 cut(s) 712
BsaXI ACNNNNNCTCC 2 cut(s) 1070, 1100
Bsc4I CCNNNNNNNGG 4 cut(s) 489, 608, 827, 845
Bse118I RCCGGY 1 cut(s) 128
Bse1I ACTGG 3 cut(s) 526, 821, 1043
BseBI CCWGG 1 cut(s) 1098
BseCI ATCGAT 1 cut(s) 887
BseDI CCNNGG 1 cut(s) 712
BseGI GGATG 2 cut(s) 677, 1195
BseLI CCNNNNNNNGG 4 cut(s) 489, 608, 827, 845
BseMII CTCAG 2 cut(s) 354, 414
BseNI ACTGG 3 cut(s) 526, 821, 1043
BseSI GKGCMC 1 cut(s) 800
BseXI GCAGC 5 cut(s) 90, 305, 671, 1147, 1166
BsgI GTGCAG 1 cut(s) 304
Bsh1285I CGRYCG 1 cut(s) 712
BshFI GGCC 2 cut(s) 503, 525
BshVI ATCGAT 1 cut(s) 887
BsiEI CGRYCG 1 cut(s) 712
BsiHKAI GWGCWC 2 cut(s) 625, 800
BsiSI CCGG 1 cut(s) 129
BslI CCNNNNNNNGG 4 cut(s) 489, 608, 827, 845
BsmAI GTCTC 2 cut(s) 170, 1126
BsnI GGCC 2 cut(s) 503, 525
Bso31I GGTCTC 2 cut(s) 170, 1126
Bsp1286I GDGCHC 2 cut(s) 625, 800
Bsp143I GATC 5 cut(s) 136, 652, 749, 779, 1057
Bsp1720I GCTNAGC 1 cut(s) 368
BspACI CCGC 3 cut(s) 76, 357, 596
BspANI GGCC 2 cut(s) 503, 525
BspCNI CTCAG 2 cut(s) 355, 413
BspDI ATCGAT 1 cut(s) 887
BspHI TCATGA 1 cut(s) 1225
BspLI GGNNCC 3 cut(s) 133, 751, 862
BspPI GGATC 2 cut(s) 744, 757
BspQI GCTCTTC 1 cut(s) 538
BspTNI GGTCTC 2 cut(s) 170, 1126
BsrBI CCGCTC 1 cut(s) 359
BsrFI RCCGGY 1 cut(s) 128
BsrI ACTGG 3 cut(s) 526, 821, 1043
BssAI RCCGGY 1 cut(s) 128
BssECI CCNNGG 1 cut(s) 712
BssMI GATC 5 cut(s) 136, 652, 749, 779, 1057
BssSI CACGAG 1 cut(s) 262
Bst2BI CACGAG 1 cut(s) 262
Bst2UI CCWGG 1 cut(s) 1098
Bst4CI ACNGT 5 cut(s) 193, 304, 427, 713, 982
Bst6I CTCTTC 3 cut(s) 91, 538, 1220
BstAPI GCANNNNNTGC 2 cut(s) 353, 889
BstDEI CTNAG 3 cut(s) 363, 368, 400
BstDSI CCRYGG 1 cut(s) 712
BstF5I GGATG 2 cut(s) 677, 1195
BstH2I RGCGCY 1 cut(s) 777
BstHHI GCGC 2 cut(s) 691, 776
BstKTI GATC 5 cut(s) 139, 655, 752, 782, 1060
BstMAI GTCTC 2 cut(s) 170, 1126
BstMBI GATC 5 cut(s) 136, 652, 749, 779, 1057
BstMCI CGRYCG 1 cut(s) 712
BstMWI GCNNNNNNNGC 5 cut(s) 315, 353, 869, 889, 968
BstNI CCWGG 1 cut(s) 1098
BstNSI RCATGY 1 cut(s) 1006
BstSCI CCNGG 1 cut(s) 1096
BstSLI GKGCMC 1 cut(s) 800
BstV1I GCAGC 5 cut(s) 90, 305, 671, 1147, 1166
BstV2I GAAGAC 2 cut(s) 724, 1214
BstX2I RGATCY 1 cut(s) 749
BstYI RGATCY 1 cut(s) 749
Bsu15I ATCGAT 1 cut(s) 887
BsuRI GGCC 2 cut(s) 503, 525
BsuTUI ATCGAT 1 cut(s) 887
BtgI CCRYGG 1 cut(s) 712
BtrI CACGTC 1 cut(s) 247
BtsCI GGATG 2 cut(s) 677, 1195
BtsI GCAGTG 3 cut(s) 477, 561, 636
BtsIMutI CAGTG 6 cut(s) 477, 561, 636, 828, 839, 978
CciI TCATGA 1 cut(s) 1225
CfoI GCGC 2 cut(s) 691, 776
Cfr10I RCCGGY 1 cut(s) 128
Cfr13I GGNCC 1 cut(s) 1094
ClaI ATCGAT 1 cut(s) 887
Csp6I GTAC 3 cut(s) 405, 519, 1000
CviAII CATG 3 cut(s) 145, 1003, 1226
CviQI GTAC 3 cut(s) 405, 519, 1000
DdeI CTNAG 3 cut(s) 363, 368, 400
DpnI GATC 5 cut(s) 138, 654, 751, 781, 1059
DpnII GATC 5 cut(s) 136, 652, 749, 779, 1057
DrdI GACNNNNNNGTC 1 cut(s) 184
DseDI GACNNNNNNGTC 1 cut(s) 184
EaeI YGGCCR 2 cut(s) 501, 523
Eam1104I CTCTTC 3 cut(s) 91, 538, 1220
EarI CTCTTC 3 cut(s) 91, 538, 1220
EciI GGCGGA 1 cut(s) 585
Eco31I GGTCTC 2 cut(s) 170, 1126
Eco47I GGWCC 1 cut(s) 1094
Eco47III AGCGCT 1 cut(s) 775
Eco57I CTGAAG 4 cut(s) 519, 1005, 1170, 1201
EcoRI GAATTC 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 1096
FaeI CATG 3 cut(s) 148, 1006, 1229
FatI CATG 3 cut(s) 144, 1002, 1225
FauNDI CATATG 1 cut(s) 1239
Fnu4HI GCNGC 6 cut(s) 79, 319, 357, 660, 1136, 1155
FokI GGATG 2 cut(s) 684, 1202
Fsp4HI GCNGC 6 cut(s) 79, 319, 357, 660, 1136, 1155
FspBI CTAG 4 cut(s) 53, 777, 1053, 1270
GlaI GCGC 2 cut(s) 690, 775
GluI GCNGC 6 cut(s) 79, 319, 357, 660, 1136, 1155
GsuI CTGGAG 2 cut(s) 669, 1060
HaeII RGCGCY 1 cut(s) 777
HaeIII GGCC 2 cut(s) 503, 525
HapII CCGG 1 cut(s) 129
HhaI GCGC 2 cut(s) 691, 776
Hin1II CATG 3 cut(s) 148, 1006, 1229
Hin6I GCGC 2 cut(s) 689, 774
HinP1I GCGC 2 cut(s) 689, 774
HincII GTYRAC 1 cut(s) 189
HindII GTYRAC 1 cut(s) 189
HinfI GANTC 4 cut(s) 398, 497, 835, 1145
HpaII CCGG 1 cut(s) 129
HphI GGTGA 2 cut(s) 215, 433
Hpy166II GTNNAC 3 cut(s) 189, 798, 1257
Hpy188I TCNGA 7 cut(s) 136, 277, 770, 1009, 1024, 1062, 1150
Hpy188III TCNNGA 5 cut(s) 210, 362, 648, 1053, 1226
Hpy8I GTNNAC 3 cut(s) 189, 798, 1257
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 3 cut(s) 412, 873, 1059
HpyCH4III ACNGT 5 cut(s) 193, 304, 427, 713, 982
HpyCH4IV ACGT 3 cut(s) 246, 998, 1011
HpyCH4V TGCA 9 cut(s) 50, 71, 321, 340, 798, 892, 924, 1089, 1243
HpyF10VI GCNNNNNNNGC 5 cut(s) 315, 353, 869, 889, 968
HpyF3I CTNAG 3 cut(s) 363, 368, 400
HpySE526I ACGT 3 cut(s) 246, 998, 1011
Hsp92II CATG 3 cut(s) 148, 1006, 1229
HspAI GCGC 2 cut(s) 689, 774
Kzo9I GATC 5 cut(s) 136, 652, 749, 779, 1057
LguI GCTCTTC 1 cut(s) 538
Lsp1109I GCAGC 5 cut(s) 90, 305, 671, 1147, 1166
LweI GCATC 2 cut(s) 343, 879
MaeI CTAG 4 cut(s) 53, 777, 1053, 1270
MaeII ACGT 3 cut(s) 246, 998, 1011
MaeIII GTNAC 3 cut(s) 288, 421, 1043
MalI GATC 5 cut(s) 138, 654, 751, 781, 1059
MbiI CCGCTC 1 cut(s) 359
MboI GATC 5 cut(s) 136, 652, 749, 779, 1057
MboII GAAGA 8 cut(s) 13, 78, 501, 525, 729, 1207, 1214, 1241
MflI RGATCY 1 cut(s) 749
MhlI GDGCHC 2 cut(s) 625, 800
MluCI AATT 8 cut(s) 278, 445, 505, 556, 615, 805, 919, 1246
MlyI GAGTC 2 cut(s) 392, 491
MmeI TCCRAC 2 cut(s) 138, 1010
MnlI CCTC 4 cut(s) 106, 225, 784, 926
MroXI GAANNNNTTC 1 cut(s) 532
MseI TTAA 5 cut(s) 311, 429, 474, 582, 804
MslI CAYNNNNRTG 1 cut(s) 983
MspI CCGG 1 cut(s) 129
MspR9I CCNGG 1 cut(s) 1098
MvaI CCWGG 1 cut(s) 1098
MwoI GCNNNNNNNGC 5 cut(s) 315, 353, 869, 889, 968
NdeI CATATG 1 cut(s) 1239
NdeII GATC 5 cut(s) 136, 652, 749, 779, 1057
NlaIII CATG 3 cut(s) 148, 1006, 1229
NlaIV GGNNCC 3 cut(s) 133, 751, 862
NmeAIII GCCGAG 1 cut(s) 479
NmuCI GTSAC 3 cut(s) 288, 421, 1043
NspI RCATGY 1 cut(s) 1006
OliI CACNNNNGTG 1 cut(s) 983
PagI TCATGA 1 cut(s) 1225
PciI ACATGT 1 cut(s) 1002
PciSI GCTCTTC 1 cut(s) 538
PdmI GAANNNNTTC 1 cut(s) 532
PfeI GAWTC 2 cut(s) 835, 1145
PflMI CCANNNNNTGG 1 cut(s) 489
PkrI GCNGC 6 cut(s) 80, 320, 358, 661, 1137, 1156
PleI GAGTC 2 cut(s) 392, 491
PpsI GAGTC 2 cut(s) 392, 491
PscI ACATGT 1 cut(s) 1002
PshBI ATTAAT 1 cut(s) 804
Psp6I CCWGG 1 cut(s) 1096
PspGI CCWGG 1 cut(s) 1096
PspN4I GGNNCC 3 cut(s) 133, 751, 862
PspPI GGNCC 1 cut(s) 1094
PsuI RGATCY 1 cut(s) 749
RsaI GTAC 3 cut(s) 406, 520, 1001
RsaNI GTAC 3 cut(s) 405, 519, 1000
RseI CAYNNNNRTG 1 cut(s) 983
SapI GCTCTTC 1 cut(s) 538
SaqAI TTAA 5 cut(s) 311, 429, 474, 582, 804
SatI GCNGC 6 cut(s) 79, 319, 357, 660, 1136, 1155
Sau3AI GATC 5 cut(s) 136, 652, 749, 779, 1057
Sau96I GGNCC 1 cut(s) 1094
SchI GAGTC 2 cut(s) 392, 491
ScrFI CCNGG 1 cut(s) 1098
SduI GDGCHC 2 cut(s) 625, 800
SfaNI GCATC 2 cut(s) 343, 879
SinI GGWCC 1 cut(s) 1094
SmiMI CAYNNNNRTG 1 cut(s) 983
SpeI ACTAGT 1 cut(s) 52
Sse9I AATT 8 cut(s) 278, 445, 505, 556, 615, 805, 919, 1246
SsiI CCGC 3 cut(s) 76, 357, 596
SspMI CTAG 4 cut(s) 53, 777, 1053, 1270
StyD4I CCNGG 1 cut(s) 1096
TaaI ACNGT 5 cut(s) 193, 304, 427, 713, 982
TaiI ACGT 3 cut(s) 249, 1001, 1014
TaqI TCGA 4 cut(s) 396, 640, 887, 958
TasI AATT 8 cut(s) 278, 445, 505, 556, 615, 805, 919, 1246
TauI GCSGC 1 cut(s) 359
TfiI GAWTC 2 cut(s) 835, 1145
Tru1I TTAA 5 cut(s) 311, 429, 474, 582, 804
Tru9I TTAA 5 cut(s) 311, 429, 474, 582, 804
TscAI CASTG 6 cut(s) 484, 568, 636, 828, 846, 985
TseFI GTSAC 3 cut(s) 288, 421, 1043
TseI GCWGC 5 cut(s) 78, 318, 659, 1135, 1154
Tsp45I GTSAC 3 cut(s) 288, 421, 1043
TspDTI ATGAA 5 cut(s) 730, 751, 1206, 1214, 1242
TspRI CASTG 6 cut(s) 484, 568, 636, 828, 846, 985
Van91I CCANNNNNTGG 1 cut(s) 489
VneI GTGCAC 1 cut(s) 796
VpaK11BI GGWCC 1 cut(s) 1094
VspI ATTAAT 1 cut(s) 804
XapI RAATTY 1 cut(s) 278
XbaI TCTAGA 1 cut(s) 1052
XceI RCATGY 1 cut(s) 1006
XmnI GAANNNNTTC 1 cut(s) 532
XspI CTAG 4 cut(s) 53, 777, 1053, 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.