pycom12g04920

disease resistance RPP13-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001713
Physical Location & Seq
Reverse (-)
4881990 .. 4883051
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g04920.1

Sequence Viewer

Length: 1062 bp
ATGAATGGTTCTCAGCATCTGATGCAGGTAAGAGCTCATGGCGATCCAGAAAACCTGCAGACGATATTCAATGTCAATGAGTCCTATCCAGATCTTTCCAAAGTGGAGTGGTCCAAGTTGAGGAATGTGAATGTTCTTTATCTTGGAAGGTGGCACAACAGAGCTAAGCATCACATTGAAGTAGATGACACTGAATTCTTCCACGGGTTGCAGTATATGGTAAAGTTGATATTCTTCAGTCTACGAGGAATATCGAGGATCATGGAGCTTCCTGATTCTGTATGCAAGCTTGTTAGTTTGAGGATCTTGGATCTCAGTGCCTGTCACAACTTAGAGGTACTTCCGGAAAGAATAGGCTTGCTTAAGAATCTCACACACTTGGATATGTCCGGGTGTTACTTGCTAGAGCATATGCCTAAGGGGATTGCCTTGCTCTCAGAACTTCAAGTTCTTAAAGGATTCATAATTCGCGAATATCATAAGAATGATACTCCATGTACTCTTCAGGATTTGTCAGCATTGCAGAAGTTGAGGAAATTGACAATCAGCACTAGCAGGGAAGATTTTCCCAGCAAACAAGAGCTAATTGATTTCCAACATCTTAAATCACTTCGAAAGCTGACAATAGTGTGGGGAGGGTTATCCATCCAAGCCAATGCTGCACCAGAGCTGGGAGAACCAACTGGGACCAAAACTCATATTCAGGAAAATCGAGAATATTTTGAGGAACTTGAGAAGATGGACCTTCAGTGCTATCCTAGGATGACAGCACCTAGCTGGTTGGTGCCCGGTAGGCCTAAGAGCCTAAAAAAGCTCTACGTTAGAGGAGGGCCGCTTCGAAATCTAGGTCATGCGGAGGAGAATGACAAGTGGACAGTTGAGATATTACGTCTGAAGTTCTTGAGTGAATTAAAGATGGACTGGAATGAACTGCAGATGTCATTTCCACATTTAACATATGTGGAGAAATTTAGATGCCCTAGGCTCACTTTCTTCCCATGTGATGAGAGTGGAGTTTGGTTGAAACCGCGAAATGGAACAAATGAAATCATCTCGGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

354

Amino Acids

40.95

Weight (kDa)

8.1

Isoelectric Point (pI)

48.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 38 - 274 2.7e-18 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 16, 63
AccB1I GGYRCC 1 cut(s) 784
AccI GTMKAC 1 cut(s) 241
AccII CGCG 2 cut(s) 471, 1030
AccIII TCCGGA 1 cut(s) 343
AciI CCGC 3 cut(s) 833, 854, 1028
AclWI GGATC 4 cut(s) 38, 266, 311, 318
AcsI RAATTY 2 cut(s) 194, 968
AcuI CTGAAG 4 cut(s) 220, 488, 731, 914
AfaI GTAC 2 cut(s) 339, 499
AfiI CCNNNNNNNGG 3 cut(s) 120, 671, 1034
AflII CTTAAG 1 cut(s) 362
AgsI TTSAA 4 cut(s) 70, 179, 446, 1024
AluBI AGCT 9 cut(s) 35, 164, 268, 289, 583, 619, 670, 777, 814
AluI AGCT 9 cut(s) 35, 164, 268, 289, 583, 619, 670, 777, 814
Alw21I GWGCWC 1 cut(s) 37
AlwI GGATC 4 cut(s) 38, 266, 311, 318
AlwNI CAGNNNCTG 2 cut(s) 19, 321
Aor13HI TCCGGA 1 cut(s) 343
AoxI GGCC 2 cut(s) 794, 830
ApeKI GCWGC 1 cut(s) 659
ApoI RAATTY 2 cut(s) 194, 968
ArsI GACNNNNNNTTYG 2 cut(s) 832, 864
Asp700I GAANNNNTTC 1 cut(s) 564
AspA2I CCTAGG 2 cut(s) 758, 980
AspS9I GGNCC 4 cut(s) 111, 687, 742, 830
AsuC2I CCSGG 2 cut(s) 391, 789
AsuII TTCGAA 2 cut(s) 613, 838
AvaII GGWCC 3 cut(s) 111, 687, 742
AvrII CCTAGG 2 cut(s) 758, 980
AxyI CCTNAGG 1 cut(s) 417
BaeGI GKGCMC 1 cut(s) 789
BanI GGYRCC 1 cut(s) 784
BanII GRGCYC 1 cut(s) 37
Bbv12I GWGCWC 1 cut(s) 37
BbvI GCAGC 1 cut(s) 646
BccI CCATC 3 cut(s) 653, 733, 910
BcnI CCSGG 2 cut(s) 391, 789
BfaI CTAG 6 cut(s) 404, 552, 759, 774, 845, 981
BfmI CTRYAG 2 cut(s) 56, 932
BfrI CTTAAG 1 cut(s) 362
BfuAI ACCTGC 2 cut(s) 16, 63
BglI GCCNNNNNGGC 1 cut(s) 793
BglII AGATCT 1 cut(s) 91
BisI GCNGC 2 cut(s) 660, 833
BlnI CCTAGG 2 cut(s) 758, 980
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 2 cut(s) 661, 834
Bme1390I CCNGG 2 cut(s) 391, 789
Bme18I GGWCC 3 cut(s) 111, 687, 742
BmgT120I GGNCC 4 cut(s) 111, 687, 742, 830
BmiI GGNNCC 2 cut(s) 688, 786
BmrFI CCNGG 2 cut(s) 391, 789
BmrI ACTGGG 1 cut(s) 693
BmsI GCATC 4 cut(s) 12, 25, 178, 965
BmuI ACTGGG 1 cut(s) 693
Bpu1102I GCTNAGC 1 cut(s) 165
Bpu14I TTCGAA 2 cut(s) 613, 838
BpuEI CTTGAG 2 cut(s) 752, 922
BpuMI CCSGG 2 cut(s) 391, 789
BsaJI CCNNGG 3 cut(s) 202, 758, 980
BsaWI WCCGGW 1 cut(s) 343
Bsc4I CCNNNNNNNGG 3 cut(s) 120, 671, 1034
Bse1I ACTGG 2 cut(s) 688, 926
Bse21I CCTNAGG 1 cut(s) 417
Bse3DI GCAATG 1 cut(s) 518
BseAI TCCGGA 1 cut(s) 343
BseDI CCNNGG 3 cut(s) 202, 758, 980
BseGI GGATG 2 cut(s) 645, 768
BseLI CCNNNNNNNGG 3 cut(s) 120, 671, 1034
BseMI GCAATG 1 cut(s) 518
BseMII CTCAG 3 cut(s) 26, 328, 450
BseNI ACTGG 2 cut(s) 688, 926
BseRI GAGGAG 2 cut(s) 840, 872
BseSI GKGCMC 1 cut(s) 789
BseXI GCAGC 1 cut(s) 646
BseYI CCCAGC 2 cut(s) 569, 670
BsgI GTGCAG 1 cut(s) 645
Bsh1236I CGCG 2 cut(s) 471, 1030
BshFI GGCC 2 cut(s) 796, 832
BshNI GGYRCC 1 cut(s) 784
BsiHKAI GWGCWC 1 cut(s) 37
BsiSI CCGG 3 cut(s) 344, 390, 789
BslFI GGGAC 1 cut(s) 700
BslI CCNNNNNNNGG 3 cut(s) 120, 671, 1034
BsmFI GGGAC 1 cut(s) 700
BsnI GGCC 2 cut(s) 796, 832
Bsp119I TTCGAA 2 cut(s) 613, 838
Bsp1286I GDGCHC 2 cut(s) 37, 789
Bsp13I TCCGGA 1 cut(s) 343
Bsp143I GATC 5 cut(s) 43, 91, 258, 303, 310
Bsp1720I GCTNAGC 1 cut(s) 165
Bsp68I TCGCGA 1 cut(s) 471
BspACI CCGC 3 cut(s) 833, 854, 1028
BspANI GGCC 2 cut(s) 796, 832
BspCNI CTCAG 3 cut(s) 25, 327, 449
BspEI TCCGGA 1 cut(s) 343
BspFNI CGCG 2 cut(s) 471, 1030
BspLI GGNNCC 2 cut(s) 688, 786
BspMAI CTGCAG 2 cut(s) 60, 936
BspMI ACCTGC 2 cut(s) 16, 63
BspPI GGATC 4 cut(s) 38, 266, 311, 318
BspT104I TTCGAA 2 cut(s) 613, 838
BspT107I GGYRCC 1 cut(s) 784
BspTI CTTAAG 1 cut(s) 362
BsrDI GCAATG 1 cut(s) 518
BsrI ACTGG 2 cut(s) 688, 926
BssECI CCNNGG 3 cut(s) 202, 758, 980
BssMI GATC 5 cut(s) 43, 91, 258, 303, 310
BssT1I CCWWGG 2 cut(s) 758, 980
Bst4CI ACNGT 1 cut(s) 877
Bst6I CTCTTC 1 cut(s) 507
BstAFI CTTAAG 1 cut(s) 362
BstAPI GCANNNNNTGC 1 cut(s) 22
BstBI TTCGAA 2 cut(s) 613, 838
BstC8I GCNNGC 2 cut(s) 287, 359
BstDEI CTNAG 7 cut(s) 12, 165, 314, 331, 417, 436, 798
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 2 cut(s) 645, 768
BstFNI CGCG 2 cut(s) 471, 1030
BstKTI GATC 5 cut(s) 46, 94, 261, 306, 313
BstMBI GATC 5 cut(s) 43, 91, 258, 303, 310
BstMWI GCNNNNNNNGC 3 cut(s) 22, 659, 793
BstSCI CCNGG 2 cut(s) 389, 787
BstSFI CTRYAG 2 cut(s) 56, 932
BstSLI GKGCMC 1 cut(s) 789
BstUI CGCG 2 cut(s) 471, 1030
BstV1I GCAGC 1 cut(s) 646
BstX2I RGATCY 3 cut(s) 91, 303, 310
BstYI RGATCY 3 cut(s) 91, 303, 310
Bsu36I CCTNAGG 1 cut(s) 417
BsuRI GGCC 2 cut(s) 796, 832
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 2 cut(s) 645, 768
BtsIMutI CAGTG 3 cut(s) 189, 322, 755
BtuMI TCGCGA 1 cut(s) 471
BveI ACCTGC 2 cut(s) 16, 63
Cac8I GCNNGC 2 cut(s) 287, 359
CaiI CAGNNNCTG 2 cut(s) 19, 321
Cfr13I GGNCC 4 cut(s) 111, 687, 742, 830
Csp6I GTAC 2 cut(s) 338, 498
CviAII CATG 5 cut(s) 38, 262, 495, 851, 999
CviQI GTAC 2 cut(s) 338, 498
DdeI CTNAG 7 cut(s) 12, 165, 314, 331, 417, 436, 798
DpnI GATC 5 cut(s) 45, 93, 260, 305, 312
DpnII GATC 5 cut(s) 43, 91, 258, 303, 310
Eam1104I CTCTTC 1 cut(s) 507
EarI CTCTTC 1 cut(s) 507
Ecl136II GAGCTC 1 cut(s) 35
Eco130I CCWWGG 2 cut(s) 758, 980
Eco147I AGGCCT 1 cut(s) 796
Eco24I GRGCYC 1 cut(s) 37
Eco47I GGWCC 3 cut(s) 111, 687, 742
Eco53kI GAGCTC 1 cut(s) 35
Eco57I CTGAAG 4 cut(s) 220, 488, 731, 914
Eco81I CCTNAGG 1 cut(s) 417
EcoICRI GAGCTC 1 cut(s) 35
EcoRI GAATTC 1 cut(s) 194
EcoT14I CCWWGG 2 cut(s) 758, 980
EcoT38I GRGCYC 1 cut(s) 37
ErhI CCWWGG 2 cut(s) 758, 980
FaeI CATG 5 cut(s) 41, 265, 498, 854, 1002
FaqI GGGAC 1 cut(s) 700
FatI CATG 5 cut(s) 37, 261, 494, 850, 998
FauNDI CATATG 2 cut(s) 411, 958
FblI GTMKAC 1 cut(s) 241
Fnu4HI GCNGC 2 cut(s) 660, 833
FokI GGATG 2 cut(s) 632, 775
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 2 cut(s) 660, 833
FspBI CTAG 6 cut(s) 404, 552, 759, 774, 845, 981
GluI GCNGC 2 cut(s) 660, 833
GsaI CCCAGC 2 cut(s) 573, 674
HaeIII GGCC 2 cut(s) 796, 832
HapII CCGG 3 cut(s) 344, 390, 789
Hin1II CATG 5 cut(s) 41, 265, 498, 854, 1002
HindIII AAGCTT 1 cut(s) 287
HinfI GANTC 4 cut(s) 80, 275, 367, 459
HpaII CCGG 3 cut(s) 344, 390, 789
Hpy166II GTNNAC 2 cut(s) 242, 873
Hpy188I TCNGA 3 cut(s) 21, 439, 894
Hpy188III TCNNGA 9 cut(s) 47, 89, 272, 344, 470, 506, 704, 713, 901
Hpy8I GTNNAC 2 cut(s) 242, 873
HpyAV CCTTC 2 cut(s) 141, 755
HpyCH4III ACNGT 1 cut(s) 877
HpyCH4IV ACGT 2 cut(s) 819, 889
HpyCH4V TGCA 7 cut(s) 25, 58, 211, 285, 523, 662, 934
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 659, 793
HpyF3I CTNAG 7 cut(s) 12, 165, 314, 331, 417, 436, 798
HpySE526I ACGT 2 cut(s) 819, 889
Hsp92II CATG 5 cut(s) 41, 265, 498, 854, 1002
Kpn2I TCCGGA 1 cut(s) 343
Kzo9I GATC 5 cut(s) 43, 91, 258, 303, 310
LmnI GCTCC 1 cut(s) 265
Lsp1109I GCAGC 1 cut(s) 646
LweI GCATC 4 cut(s) 12, 25, 178, 965
MaeI CTAG 6 cut(s) 404, 552, 759, 774, 845, 981
MaeII ACGT 2 cut(s) 819, 889
MaeIII GTNAC 2 cut(s) 323, 395
MalI GATC 5 cut(s) 45, 93, 260, 305, 312
MboI GATC 5 cut(s) 43, 91, 258, 303, 310
MboII GAAGA 6 cut(s) 190, 226, 494, 572, 748, 985
MflI RGATCY 3 cut(s) 91, 303, 310
MhlI GDGCHC 2 cut(s) 37, 789
MluCI AATT 6 cut(s) 194, 465, 536, 585, 908, 968
MlyI GAGTC 1 cut(s) 89
MmeI TCCRAC 1 cut(s) 619
MroI TCCGGA 1 cut(s) 343
MroXI GAANNNNTTC 1 cut(s) 564
MseI TTAA 5 cut(s) 363, 453, 603, 911, 953
MslI CAYNNNNRTG 1 cut(s) 483
MspCI CTTAAG 1 cut(s) 362
MspI CCGG 3 cut(s) 344, 390, 789
MspR9I CCNGG 2 cut(s) 391, 789
MvnI CGCG 2 cut(s) 471, 1030
MwoI GCNNNNNNNGC 3 cut(s) 22, 659, 793
NciI CCSGG 2 cut(s) 391, 789
NdeI CATATG 2 cut(s) 411, 958
NdeII GATC 5 cut(s) 43, 91, 258, 303, 310
NlaIII CATG 5 cut(s) 41, 265, 498, 854, 1002
NlaIV GGNNCC 2 cut(s) 688, 786
NmeAIII GCCGAG 1 cut(s) 1034
NmuCI GTSAC 1 cut(s) 323
NruI TCGCGA 1 cut(s) 471
NspV TTCGAA 2 cut(s) 613, 838
PceI AGGCCT 1 cut(s) 796
PdmI GAANNNNTTC 1 cut(s) 564
PfeI GAWTC 3 cut(s) 275, 367, 459
PkrI GCNGC 2 cut(s) 661, 834
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
Psp124BI GAGCTC 1 cut(s) 37
PspFI CCCAGC 2 cut(s) 569, 670
PspN4I GGNNCC 2 cut(s) 688, 786
PspPI GGNCC 4 cut(s) 111, 687, 742, 830
PstI CTGCAG 2 cut(s) 60, 936
PstNI CAGNNNCTG 2 cut(s) 19, 321
PsuI RGATCY 3 cut(s) 91, 303, 310
RruI TCGCGA 1 cut(s) 471
RsaI GTAC 2 cut(s) 339, 499
RsaNI GTAC 2 cut(s) 338, 498
RseI CAYNNNNRTG 1 cut(s) 483
SacI GAGCTC 1 cut(s) 37
SaqAI TTAA 5 cut(s) 363, 453, 603, 911, 953
SatI GCNGC 2 cut(s) 660, 833
Sau3AI GATC 5 cut(s) 43, 91, 258, 303, 310
Sau96I GGNCC 4 cut(s) 111, 687, 742, 830
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 2 cut(s) 391, 789
SduI GDGCHC 2 cut(s) 37, 789
SfaNI GCATC 4 cut(s) 12, 25, 178, 965
SfcI CTRYAG 2 cut(s) 56, 932
SfuI TTCGAA 2 cut(s) 613, 838
SinI GGWCC 3 cut(s) 111, 687, 742
SmiMI CAYNNNNRTG 1 cut(s) 483
SmlI CTYRAG 3 cut(s) 362, 731, 901
SmoI CTYRAG 3 cut(s) 362, 731, 901
Sse9I AATT 6 cut(s) 194, 465, 536, 585, 908, 968
SseBI AGGCCT 1 cut(s) 796
SsiI CCGC 3 cut(s) 833, 854, 1028
SspI AATATT 1 cut(s) 719
SspMI CTAG 6 cut(s) 404, 552, 759, 774, 845, 981
SstI GAGCTC 1 cut(s) 37
StuI AGGCCT 1 cut(s) 796
StyD4I CCNGG 2 cut(s) 389, 787
StyI CCWWGG 2 cut(s) 758, 980
TaaI ACNGT 1 cut(s) 877
TaiI ACGT 2 cut(s) 822, 892
TaqI TCGA 4 cut(s) 254, 613, 712, 838
TasI AATT 6 cut(s) 194, 465, 536, 585, 908, 968
TatI WGTACW 1 cut(s) 497
TauI GCSGC 1 cut(s) 835
TfiI GAWTC 3 cut(s) 275, 367, 459
Tru1I TTAA 5 cut(s) 363, 453, 603, 911, 953
Tru9I TTAA 5 cut(s) 363, 453, 603, 911, 953
TscAI CASTG 3 cut(s) 196, 322, 755
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 1 cut(s) 659
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 4 cut(s) 17, 451, 942, 1059
TspRI CASTG 3 cut(s) 196, 322, 755
Vha464I CTTAAG 1 cut(s) 362
VpaK11BI GGWCC 3 cut(s) 111, 687, 742
XapI RAATTY 2 cut(s) 194, 968
XmaJI CCTAGG 2 cut(s) 758, 980
XmiI GTMKAC 1 cut(s) 241
XmnI GAANNNNTTC 1 cut(s) 564
XspI CTAG 6 cut(s) 404, 552, 759, 774, 845, 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.