Rorug04G0417900

disease resistance RPP13-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000696
Physical Location & Seq
Forward (+)
57880356 .. 57885014
4659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0417900.1

Sequence Viewer

Length: 1272 bp
ATGGCACGCAAGAAGATCCGAGAGTACGACTCCAAGAGGTTGTTGAAGGAACACTTCAAGAGGATCGCTGGCCGTGAATTGCCTCTCAAATCTGCCCAGATTACCGAATCAACTGATTTCAATGAGCTACTTGAGAAGGAGACTTGGCTCTCTACTTCCAAGTTGGTTGTCAAGCCTGACATGTTGTTTGGAAAGCGTGGGAAGAGTGGTTTGGTTGCCTTGAACCTGGATTTCGCTCAAGTCGCCACCTTTGTGAAGGAGCGCCTAGGCAAAGAGGTTGAGATGGGTGGCTGCAAAGGACCCATCACAACATTCATTGTTGAGCCCTTCATTCCCCACAATGAAGAGTTTTACATTAACATCGTCTCAGATAGACTTGGGAATAGCATAAGCTTCTCAGAGTGTGGAGGAATTGACATTGAGGAGAACTGGGATAAGGTCAAGACCATATTTGTCCCAACGGGAGCTTCCCTTACACCAGATGTATCTGCTCCACTTGTTGCAACCCTTCCCTTGGAGATTAAGAGCGAAATTGAGGAGTTTATCAAGTCCGTATTTGCTCTATTTCAAGATCTCGACTTCACTTTTCTGGAGATGAATCCTTTTGCATTGGTTGATGGAAAGCCATATCCTTTGGATATGAGAGGCGAGCTGGATGACACTGCTGCTTTCAAGAACTTCAAAAAGTGGGGCAATATTGAATTTCCAATGCCATTTGGAAGAGTTATGAGTTCGACAGAAAAGTTTATTCACGGGCTAGATGAAAAGACAAGTGCATCTTTGAAATTTACAGTTCTGAACCCTAAGGGACGAATTTGGACTATGGTTGCTGGAGGAGGTGCAAGTGTTATTTACGCAGACACGGTTGGTGATCTTGGCTTTGCTAATGAGCTCGGAAACTATGCTGAATATAGTGGAGCACCAAATGAAGAGGAGGTATTGCAGTATGCCAGAGTTGTGATTGATTGTGCAACTTCTGATCCTGATGGCCGTAAGAGAGCCCTTGTAATTGGAGGGGGAATTGCTAACTTCACTGATGTAGCTGCAACATTCAATGGCATCATTAGAGCCTTGAAGGAGAAGGAATCAAAGCTTAAAGCTGCTAGGATGCATCTTTATGTCAGGAGAGGAGGTCCCAACTACCAGAGGGGACTTGCAAAAATGAGGGCACTTGCAGAGGAAATTGGCCTCCCTATTGAGGTCTATGGTCCTGAAGCAACAATGACTGGTATTTGCAAGCAGGCAATTCAGTGCATCAGTGCTGCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

423

Amino Acids

46.72

Weight (kDa)

5.84

Isoelectric Point (pI)

34.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Citrate_synth_N PF24948 2 - 230 5.3e-75 ATP-citrate synthase ATP-grasp domain
ATP-grasp_2 PF08442 6 - 203 4.6e-17 ATP-grasp domain
Citrate_bind PF16114 241 - 417 7.6e-84 ATP citrate lyase citrate-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 10, 71, 974
AcoI YGGCCR 2 cut(s) 70, 988
AcsI RAATTY 3 cut(s) 701, 785, 813
AcuI CTGAAG 1 cut(s) 1233
AfaI GTAC 1 cut(s) 26
AfiI CCNNNNNNNGG 2 cut(s) 514, 1198
AflIII ACRYGT 1 cut(s) 180
AjnI CCWGG 1 cut(s) 225
AjuI GAANNNNNNNTTGG 4 cut(s) 194, 226, 451, 483
AleI CACNNNNGTG 1 cut(s) 251
AluBI AGCT 8 cut(s) 127, 393, 467, 652, 892, 1043, 1093, 1100
AluI AGCT 8 cut(s) 127, 393, 467, 652, 892, 1043, 1093, 1100
Alw21I GWGCWC 2 cut(s) 894, 922
Alw26I GTCTC 2 cut(s) 134, 370
AlwI GGATC 3 cut(s) 10, 71, 974
AoxI GGCC 3 cut(s) 70, 988, 1186
ApeKI GCWGC 6 cut(s) 291, 665, 1043, 1100, 1262, 1265
ApoI RAATTY 3 cut(s) 701, 785, 813
ArsI GACNNNNNNTTYG 2 cut(s) 170, 202
AspA2I CCTAGG 1 cut(s) 265
AspLEI GCGC 1 cut(s) 264
AspS9I GGNCC 3 cut(s) 299, 1133, 1208
AsuHPI GGTGA 1 cut(s) 881
AvaII GGWCC 3 cut(s) 299, 1133, 1208
AvrII CCTAGG 1 cut(s) 265
AxyI CCTNAGG 1 cut(s) 804
BaeGI GKGCMC 1 cut(s) 1171
BanII GRGCYC 3 cut(s) 327, 894, 1003
Bbv12I GWGCWC 2 cut(s) 894, 922
BbvI GCAGC 6 cut(s) 278, 652, 1030, 1087, 1249, 1252
BccI CCATC 4 cut(s) 277, 311, 611, 980
BceAI ACGGC 2 cut(s) 57, 975
BciT130I CCWGG 1 cut(s) 227
BcoDI GTCTC 2 cut(s) 134, 370
BfaI CTAG 3 cut(s) 266, 758, 1104
BfoI RGCGCY 1 cut(s) 265
BglII AGATCT 1 cut(s) 571
BisI GCNGC 6 cut(s) 292, 666, 1044, 1101, 1263, 1266
BlnI CCTAGG 1 cut(s) 265
BlsI GCNGC 6 cut(s) 293, 667, 1045, 1102, 1264, 1267
Bme1390I CCNGG 1 cut(s) 227
Bme18I GGWCC 3 cut(s) 299, 1133, 1208
BmgT120I GGNCC 3 cut(s) 299, 1133, 1208
BmiI GGNNCC 2 cut(s) 301, 1135
BmrFI CCNGG 1 cut(s) 227
BmrI ACTGGG 1 cut(s) 439
BmsI GCATC 5 cut(s) 785, 1068, 1098, 1120, 1263
BmuI ACTGGG 1 cut(s) 439
BplI GAGNNNNNCTC 2 cut(s) 14, 46
BpmI CTGGAG 2 cut(s) 611, 852
BpuEI CTTGAG 2 cut(s) 152, 222
BsaJI CCNNGG 2 cut(s) 265, 513
BsaXI ACNNNNNCTCC 2 cut(s) 1117, 1147
Bsc4I CCNNNNNNNGG 2 cut(s) 514, 1198
Bse1I ACTGG 2 cut(s) 434, 1231
Bse21I CCTNAGG 1 cut(s) 804
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 2 cut(s) 265, 513
BseGI GGATG 2 cut(s) 661, 1113
BseLI CCNNNNNNNGG 2 cut(s) 514, 1198
BseMII CTCAG 2 cut(s) 381, 411
BseNI ACTGG 2 cut(s) 434, 1231
BseRI GAGGAG 5 cut(s) 437, 551, 849, 947, 1143
BseSI GKGCMC 1 cut(s) 1171
BseXI GCAGC 6 cut(s) 278, 652, 1030, 1087, 1249, 1252
BshFI GGCC 3 cut(s) 72, 990, 1188
BsiHKAI GWGCWC 2 cut(s) 894, 922
BslFI GGGAC 4 cut(s) 440, 822, 1119, 1164
BslI CCNNNNNNNGG 2 cut(s) 514, 1198
BsmAI GTCTC 2 cut(s) 134, 370
BsmBI CGTCTC 1 cut(s) 370
BsmFI GGGAC 4 cut(s) 440, 822, 1119, 1164
BsnI GGCC 3 cut(s) 72, 990, 1188
Bsp1286I GDGCHC 5 cut(s) 327, 894, 922, 1003, 1171
Bsp143I GATC 5 cut(s) 15, 63, 571, 871, 979
BspANI GGCC 3 cut(s) 72, 990, 1188
BspCNI CTCAG 2 cut(s) 380, 410
BspLI GGNNCC 2 cut(s) 301, 1135
BspPI GGATC 3 cut(s) 10, 71, 974
BsrI ACTGG 2 cut(s) 434, 1231
BssECI CCNNGG 2 cut(s) 265, 513
BssMI GATC 5 cut(s) 15, 63, 571, 871, 979
BssT1I CCWWGG 2 cut(s) 265, 513
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 2 cut(s) 793, 865
Bst6I CTCTTC 4 cut(s) 197, 339, 715, 924
BstC8I GCNNGC 5 cut(s) 7, 70, 650, 1238, 1242
BstDEI CTNAG 3 cut(s) 367, 397, 804
BstF5I GGATG 2 cut(s) 661, 1113
BstH2I RGCGCY 1 cut(s) 265
BstHHI GCGC 1 cut(s) 264
BstKTI GATC 5 cut(s) 18, 66, 574, 874, 982
BstMAI GTCTC 2 cut(s) 134, 370
BstMBI GATC 5 cut(s) 15, 63, 571, 871, 979
BstMWI GCNNNNNNNGC 1 cut(s) 242
BstNI CCWGG 1 cut(s) 227
BstNSI RCATGY 1 cut(s) 184
BstSCI CCNGG 1 cut(s) 225
BstSLI GKGCMC 1 cut(s) 1171
BstV1I GCAGC 6 cut(s) 278, 652, 1030, 1087, 1249, 1252
BstX2I RGATCY 2 cut(s) 15, 571
BstYI RGATCY 2 cut(s) 15, 571
Bsu36I CCTNAGG 1 cut(s) 804
BsuRI GGCC 3 cut(s) 72, 990, 1188
BtsCI GGATG 2 cut(s) 661, 1113
BtsI GCAGTG 1 cut(s) 660
BtsIMutI CAGTG 4 cut(s) 660, 1032, 1256, 1264
Cac8I GCNNGC 5 cut(s) 7, 70, 650, 1238, 1242
CfoI GCGC 1 cut(s) 264
Cfr13I GGNCC 3 cut(s) 299, 1133, 1208
Csp6I GTAC 1 cut(s) 25
CviAII CATG 2 cut(s) 181, 1269
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 3 cut(s) 367, 397, 804
DpnI GATC 5 cut(s) 17, 65, 573, 873, 981
DpnII GATC 5 cut(s) 15, 63, 571, 871, 979
EaeI YGGCCR 2 cut(s) 70, 988
Eam1104I CTCTTC 4 cut(s) 197, 339, 715, 924
EarI CTCTTC 4 cut(s) 197, 339, 715, 924
Ecl136II GAGCTC 1 cut(s) 892
Eco130I CCWWGG 2 cut(s) 265, 513
Eco24I GRGCYC 3 cut(s) 327, 894, 1003
Eco47I GGWCC 3 cut(s) 299, 1133, 1208
Eco53kI GAGCTC 1 cut(s) 892
Eco57I CTGAAG 1 cut(s) 1233
Eco81I CCTNAGG 1 cut(s) 804
EcoICRI GAGCTC 1 cut(s) 892
EcoO109I RGGNCCY 2 cut(s) 299, 1133
EcoRII CCWGG 1 cut(s) 225
EcoT14I CCWWGG 2 cut(s) 265, 513
EcoT22I ATGCAT 1 cut(s) 1113
EcoT38I GRGCYC 3 cut(s) 327, 894, 1003
ErhI CCWWGG 2 cut(s) 265, 513
Esp3I CGTCTC 1 cut(s) 370
FaeI CATG 2 cut(s) 184, 1272
FalI AAGNNNNNCTT 4 cut(s) 38, 70, 763, 795
FaqI GGGAC 4 cut(s) 440, 822, 1119, 1164
FatI CATG 2 cut(s) 180, 1268
Fnu4HI GCNGC 6 cut(s) 292, 666, 1044, 1101, 1263, 1266
FokI GGATG 2 cut(s) 668, 1120
FriOI GRGCYC 3 cut(s) 327, 894, 1003
Fsp4HI GCNGC 6 cut(s) 292, 666, 1044, 1101, 1263, 1266
FspBI CTAG 3 cut(s) 266, 758, 1104
GlaI GCGC 1 cut(s) 263
GluI GCNGC 6 cut(s) 292, 666, 1044, 1101, 1263, 1266
GsuI CTGGAG 2 cut(s) 611, 852
HaeII RGCGCY 1 cut(s) 265
HaeIII GGCC 3 cut(s) 72, 990, 1188
HhaI GCGC 1 cut(s) 264
Hin1II CATG 2 cut(s) 184, 1272
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HindIII AAGCTT 2 cut(s) 391, 1091
HinfI GANTC 4 cut(s) 29, 107, 598, 1085
HphI GGTGA 1 cut(s) 881
Hpy188I TCNGA 6 cut(s) 20, 370, 400, 798, 896, 979
Hpy188III TCNNGA 9 cut(s) 58, 442, 569, 575, 590, 673, 983, 1123, 1211
HpyAV CCTTC 7 cut(s) 40, 130, 250, 337, 518, 1069, 1075
HpyCH4III ACNGT 2 cut(s) 793, 865
HpyF10VI GCNNNNNNNGC 1 cut(s) 242
HpyF3I CTNAG 3 cut(s) 367, 397, 804
Hsp92II CATG 2 cut(s) 184, 1272
HspAI GCGC 1 cut(s) 262
Kzo9I GATC 5 cut(s) 15, 63, 571, 871, 979
LmnI GCTCC 4 cut(s) 259, 464, 496, 917
Lsp1109I GCAGC 6 cut(s) 278, 652, 1030, 1087, 1249, 1252
LweI GCATC 5 cut(s) 785, 1068, 1098, 1120, 1263
MaeI CTAG 3 cut(s) 266, 758, 1104
MalI GATC 5 cut(s) 17, 65, 573, 873, 981
MboI GATC 5 cut(s) 15, 63, 571, 871, 979
MboII GAAGA 5 cut(s) 25, 214, 356, 732, 941
MflI RGATCY 2 cut(s) 15, 571
MhlI GDGCHC 5 cut(s) 327, 894, 922, 1003, 1171
MlyI GAGTC 1 cut(s) 23
Mph1103I ATGCAT 1 cut(s) 1113
MseI TTAA 3 cut(s) 357, 522, 1095
MslI CAYNNNNRTG 2 cut(s) 251, 1116
MspR9I CCNGG 1 cut(s) 227
MvaI CCWGG 1 cut(s) 227
MwoI GCNNNNNNNGC 1 cut(s) 242
NdeII GATC 5 cut(s) 15, 63, 571, 871, 979
NlaIII CATG 2 cut(s) 184, 1272
NlaIV GGNNCC 2 cut(s) 301, 1135
NsiI ATGCAT 1 cut(s) 1113
NspI RCATGY 1 cut(s) 184
OliI CACNNNNGTG 1 cut(s) 251
PciI ACATGT 1 cut(s) 180
PfeI GAWTC 3 cut(s) 107, 598, 1085
PkrI GCNGC 6 cut(s) 293, 667, 1045, 1102, 1264, 1267
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
PpuMI RGGWCCY 2 cut(s) 299, 1133
PscI ACATGT 1 cut(s) 180
Psp124BI GAGCTC 1 cut(s) 894
Psp5II RGGWCCY 2 cut(s) 299, 1133
Psp6I CCWGG 1 cut(s) 225
PspGI CCWGG 1 cut(s) 225
PspN4I GGNNCC 2 cut(s) 301, 1135
PspPI GGNCC 3 cut(s) 299, 1133, 1208
PspPPI RGGWCCY 2 cut(s) 299, 1133
PsuI RGATCY 2 cut(s) 15, 571
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
RseI CAYNNNNRTG 2 cut(s) 251, 1116
SacI GAGCTC 1 cut(s) 894
SaqAI TTAA 3 cut(s) 357, 522, 1095
SatI GCNGC 6 cut(s) 292, 666, 1044, 1101, 1263, 1266
Sau3AI GATC 5 cut(s) 15, 63, 571, 871, 979
Sau96I GGNCC 3 cut(s) 299, 1133, 1208
SchI GAGTC 1 cut(s) 23
ScrFI CCNGG 1 cut(s) 227
SduI GDGCHC 5 cut(s) 327, 894, 922, 1003, 1171
SfaNI GCATC 5 cut(s) 785, 1068, 1098, 1120, 1263
SinI GGWCC 3 cut(s) 299, 1133, 1208
SmiMI CAYNNNNRTG 2 cut(s) 251, 1116
SmlI CTYRAG 2 cut(s) 131, 237
SmoI CTYRAG 2 cut(s) 131, 237
SspI AATATT 1 cut(s) 697
SspMI CTAG 3 cut(s) 266, 758, 1104
SstI GAGCTC 1 cut(s) 894
StyD4I CCNGG 1 cut(s) 225
StyI CCWWGG 2 cut(s) 265, 513
TaaI ACNGT 2 cut(s) 793, 865
TaqI TCGA 2 cut(s) 576, 734
TfiI GAWTC 3 cut(s) 107, 598, 1085
Tru1I TTAA 3 cut(s) 357, 522, 1095
Tru9I TTAA 3 cut(s) 357, 522, 1095
TscAI CASTG 4 cut(s) 667, 1039, 1256, 1264
TseI GCWGC 6 cut(s) 291, 665, 1043, 1100, 1262, 1265
TspDTI ATGAA 6 cut(s) 304, 319, 357, 611, 777, 942
TspGWI ACGGA 1 cut(s) 541
TspRI CASTG 4 cut(s) 667, 1039, 1256, 1264
VpaK11BI GGWCC 3 cut(s) 299, 1133, 1208
XapI RAATTY 3 cut(s) 701, 785, 813
XceI RCATGY 1 cut(s) 184
XmaJI CCTAGG 1 cut(s) 265
XspI CTAG 3 cut(s) 266, 758, 1104
Zsp2I ATGCAT 1 cut(s) 1113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.