Rorug05G0560300

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000645
Physical Location & Seq
Forward (+)
75041717 .. 75044020
2304 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0560300.1

Sequence Viewer

Length: 636 bp
ATGTCAGCATATAAGAACTTAGCTACTTCTTTATCTGGGTGGAGAGTTGAAAAAATGGACCACGAGGACACTGGATGTCAAGCTGCCCCTGAAGGTCCTATATTGTGTGTAAACAATTGTGGGTTCTTTGGAAGCGCAGCTACTATGAATATGTGTTCCAAGTGCCATAAGGACTTGATGATGAAACAGGAGCAGGCTAAGCTTGCTGCATCATCAATAGGAAGTATTGTCAACGGAACCTCAAGCAGCCACGGGAAGGAACCTGTTGCTGCTAATCCAGTGGACCCAAAAACTATCTCTATGAACTCGACCATTACATTTAGGTCAGAGTACGACTCTCCTTCCCCCTTTGCTTTTGGGTCATCGGAGACTGCTGTGGCAAAGCCTGGCCCAGAATCATTTACTTTTGGGTCTGGGCAAAATGGTGAGCCGAAGCCAGAGGGCCCAAAGCGCTGCACTACCTGCAACAAGCGGGTGGGTTTAACAGGATTCAATTGTCGCTGTGGTGACCTTTTCTGTGCAGTACATCGTTATTCAGACAAACATGACTGTCCCTATGATTATCGTACTGCTGGTCGCGATGCCATTGCTAAAGCCAATCCTGTTGTCAAAGCTGAGAAGCTTGATAAAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

211

Amino Acids

22.61

Weight (kDa)

8.26

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-A20 PF01754 34 - 57 2e-12 A20-like zinc finger
zf-AN1 PF01428 152 - 188 5.8e-09 AN1-like Zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 470
AccII CGCG 1 cut(s) 579
AciI CCGC 1 cut(s) 472
AcuI CTGAAG 1 cut(s) 111
AfaI GTAC 3 cut(s) 332, 525, 568
AfeI AGCGCT 1 cut(s) 452
AfiI CCNNNNNNNGG 1 cut(s) 256
AgsI TTSAA 2 cut(s) 50, 493
AjnI CCWGG 1 cut(s) 385
AluBI AGCT 6 cut(s) 23, 83, 140, 202, 614, 622
AluI AGCT 6 cut(s) 23, 83, 140, 202, 614, 622
Alw26I GTCTC 1 cut(s) 362
Aor51HI AGCGCT 1 cut(s) 452
AoxI GGCC 2 cut(s) 388, 442
ApaI GGGCCC 1 cut(s) 446
ApeKI GCWGC 6 cut(s) 83, 137, 206, 246, 269, 453
AspLEI GCGC 2 cut(s) 137, 453
AspS9I GGNCC 6 cut(s) 58, 95, 283, 389, 442, 443
AsuHPI GGTGA 2 cut(s) 437, 518
AvaII GGWCC 3 cut(s) 58, 95, 283
BaeGI GKGCMC 1 cut(s) 446
BanII GRGCYC 1 cut(s) 446
BarI GAAGNNNNNNTAC 2 cut(s) 124, 156
BauI CACGAG 1 cut(s) 62
BbvI GCAGC 6 cut(s) 70, 149, 193, 256, 258, 440
BcgI CGANNNNNNTGC 2 cut(s) 569, 603
BciT130I CCWGG 1 cut(s) 387
BcoDI GTCTC 1 cut(s) 362
BfoI RGCGCY 1 cut(s) 454
BfuAI ACCTGC 1 cut(s) 470
BisI GCNGC 6 cut(s) 84, 138, 207, 247, 270, 454
BlpI GCTNAGC 1 cut(s) 198
BlsI GCNGC 6 cut(s) 85, 139, 208, 248, 271, 455
Bme1390I CCNGG 1 cut(s) 387
Bme18I GGWCC 3 cut(s) 58, 95, 283
BmgT120I GGNCC 6 cut(s) 58, 95, 283, 389, 442, 443
BmiI GGNNCC 4 cut(s) 238, 261, 285, 444
BmrFI CCNGG 1 cut(s) 387
BmsI GCATC 2 cut(s) 218, 571
BplI GAGNNNNNCTC 2 cut(s) 320, 352
Bpu1102I GCTNAGC 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 226
BsaJI CCNNGG 1 cut(s) 250
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse1I ACTGG 2 cut(s) 76, 278
Bse3DI GCAATG 1 cut(s) 585
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 1 cut(s) 250
BseGI GGATG 1 cut(s) 80
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMI GCAATG 1 cut(s) 585
BseMII CTCAG 1 cut(s) 606
BseNI ACTGG 2 cut(s) 76, 278
BseSI GKGCMC 1 cut(s) 446
BseXI GCAGC 6 cut(s) 70, 149, 193, 256, 258, 440
BsgI GTGCAG 2 cut(s) 439, 540
Bsh1236I CGCG 1 cut(s) 579
BshFI GGCC 2 cut(s) 390, 444
BslFI GGGAC 1 cut(s) 537
BslI CCNNNNNNNGG 1 cut(s) 256
BsmAI GTCTC 1 cut(s) 362
BsmFI GGGAC 1 cut(s) 537
BsnI GGCC 2 cut(s) 390, 444
Bsp120I GGGCCC 1 cut(s) 442
Bsp1286I GDGCHC 1 cut(s) 446
Bsp1720I GCTNAGC 1 cut(s) 198
Bsp68I TCGCGA 1 cut(s) 579
BspACI CCGC 1 cut(s) 472
BspANI GGCC 2 cut(s) 390, 444
BspCNI CTCAG 1 cut(s) 607
BspFNI CGCG 1 cut(s) 579
BspLI GGNNCC 4 cut(s) 238, 261, 285, 444
BspMI ACCTGC 1 cut(s) 470
BsrDI GCAATG 1 cut(s) 585
BsrI ACTGG 2 cut(s) 76, 278
BssECI CCNNGG 1 cut(s) 250
BssSI CACGAG 1 cut(s) 62
Bst2BI CACGAG 1 cut(s) 62
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 1 cut(s) 551
BstAPI GCANNNNNTGC 1 cut(s) 462
BstC8I GCNNGC 2 cut(s) 195, 204
BstDEI CTNAG 3 cut(s) 19, 198, 615
BstDSI CCRYGG 1 cut(s) 250
BstEII GGTNACC 1 cut(s) 506
BstF5I GGATG 1 cut(s) 80
BstFNI CGCG 1 cut(s) 579
BstH2I RGCGCY 1 cut(s) 454
BstHHI GCGC 2 cut(s) 137, 453
BstMAI GTCTC 1 cut(s) 362
BstMWI GCNNNNNNNGC 4 cut(s) 199, 203, 450, 462
BstNI CCWGG 1 cut(s) 387
BstPI GGTNACC 1 cut(s) 506
BstSCI CCNGG 1 cut(s) 385
BstSLI GKGCMC 1 cut(s) 446
BstUI CGCG 1 cut(s) 579
BstV1I GCAGC 6 cut(s) 70, 149, 193, 256, 258, 440
BsuRI GGCC 2 cut(s) 390, 444
BtgI CCRYGG 1 cut(s) 250
BtgZI GCGATG 1 cut(s) 594
BtsCI GGATG 1 cut(s) 80
BtsIMutI CAGTG 2 cut(s) 69, 285
BtuMI TCGCGA 1 cut(s) 579
BveI ACCTGC 1 cut(s) 470
Cac8I GCNNGC 2 cut(s) 195, 204
CfoI GCGC 2 cut(s) 137, 453
Cfr13I GGNCC 6 cut(s) 58, 95, 283, 389, 442, 443
Csp6I GTAC 3 cut(s) 331, 524, 567
CviAII CATG 1 cut(s) 545
CviQI GTAC 3 cut(s) 331, 524, 567
DdeI CTNAG 3 cut(s) 19, 198, 615
Eco24I GRGCYC 1 cut(s) 446
Eco47I GGWCC 3 cut(s) 58, 95, 283
Eco47III AGCGCT 1 cut(s) 452
Eco57I CTGAAG 1 cut(s) 111
Eco91I GGTNACC 1 cut(s) 506
EcoO109I RGGNCCY 2 cut(s) 95, 442
EcoO65I GGTNACC 1 cut(s) 506
EcoRII CCWGG 1 cut(s) 385
EcoT38I GRGCYC 1 cut(s) 446
FaeI CATG 1 cut(s) 548
FaqI GGGAC 1 cut(s) 537
FatI CATG 1 cut(s) 544
FauI CCCGC 1 cut(s) 465
Fnu4HI GCNGC 6 cut(s) 84, 138, 207, 247, 270, 454
FokI GGATG 1 cut(s) 87
FriOI GRGCYC 1 cut(s) 446
Fsp4HI GCNGC 6 cut(s) 84, 138, 207, 247, 270, 454
GlaI GCGC 2 cut(s) 136, 452
GluI GCNGC 6 cut(s) 84, 138, 207, 247, 270, 454
HaeII RGCGCY 1 cut(s) 454
HaeIII GGCC 2 cut(s) 390, 444
HhaI GCGC 2 cut(s) 137, 453
Hin1II CATG 1 cut(s) 548
Hin6I GCGC 2 cut(s) 135, 451
HinP1I GCGC 2 cut(s) 135, 451
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HindIII AAGCTT 2 cut(s) 200, 620
HinfI GANTC 3 cut(s) 335, 395, 489
HphI GGTGA 2 cut(s) 437, 518
Hpy166II GTNNAC 3 cut(s) 112, 232, 283
Hpy188I TCNGA 3 cut(s) 328, 367, 538
Hpy188III TCNNGA 1 cut(s) 578
Hpy8I GTNNAC 3 cut(s) 112, 232, 283
HpyAV CCTTC 3 cut(s) 86, 250, 351
HpyCH4III ACNGT 1 cut(s) 551
HpyCH4V TGCA 4 cut(s) 209, 456, 465, 521
HpyF10VI GCNNNNNNNGC 4 cut(s) 199, 203, 450, 462
HpyF3I CTNAG 3 cut(s) 19, 198, 615
Hsp92II CATG 1 cut(s) 548
HspAI GCGC 2 cut(s) 135, 451
LmnI GCTCC 1 cut(s) 190
Lsp1109I GCAGC 6 cut(s) 70, 149, 193, 256, 258, 440
LweI GCATC 2 cut(s) 218, 571
MaeIII GTNAC 1 cut(s) 506
MfeI CAATTG 2 cut(s) 115, 493
MhlI GDGCHC 1 cut(s) 446
MluCI AATT 2 cut(s) 115, 493
MlyI GAGTC 1 cut(s) 329
MnlI CCTC 3 cut(s) 58, 250, 433
MseI TTAA 1 cut(s) 482
MspR9I CCNGG 1 cut(s) 387
MunI CAATTG 2 cut(s) 115, 493
MvaI CCWGG 1 cut(s) 387
MvnI CGCG 1 cut(s) 579
MwoI GCNNNNNNNGC 4 cut(s) 199, 203, 450, 462
NlaIII CATG 1 cut(s) 548
NlaIV GGNNCC 4 cut(s) 238, 261, 285, 444
NmuCI GTSAC 1 cut(s) 506
NruI TCGCGA 1 cut(s) 579
PfeI GAWTC 2 cut(s) 395, 489
PkrI GCNGC 6 cut(s) 85, 139, 208, 248, 271, 455
PleI GAGTC 1 cut(s) 329
PpsI GAGTC 1 cut(s) 329
PpuMI RGGWCCY 1 cut(s) 95
Psp5II RGGWCCY 1 cut(s) 95
Psp6I CCWGG 1 cut(s) 385
PspEI GGTNACC 1 cut(s) 506
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 4 cut(s) 238, 261, 285, 444
PspOMI GGGCCC 1 cut(s) 442
PspPI GGNCC 6 cut(s) 58, 95, 283, 389, 442, 443
PspPPI RGGWCCY 1 cut(s) 95
RruI TCGCGA 1 cut(s) 579
RsaI GTAC 3 cut(s) 332, 525, 568
RsaNI GTAC 3 cut(s) 331, 524, 567
SaqAI TTAA 1 cut(s) 482
SatI GCNGC 6 cut(s) 84, 138, 207, 247, 270, 454
Sau96I GGNCC 6 cut(s) 58, 95, 283, 389, 442, 443
SchI GAGTC 1 cut(s) 329
ScrFI CCNGG 1 cut(s) 387
SduI GDGCHC 1 cut(s) 446
SfaNI GCATC 2 cut(s) 218, 571
SinI GGWCC 3 cut(s) 58, 95, 283
SmlI CTYRAG 1 cut(s) 241
SmoI CTYRAG 1 cut(s) 241
Sse9I AATT 2 cut(s) 115, 493
SsiI CCGC 1 cut(s) 472
StyD4I CCNGG 1 cut(s) 385
TaaI ACNGT 1 cut(s) 551
TaqI TCGA 1 cut(s) 308
TasI AATT 2 cut(s) 115, 493
TatI WGTACW 1 cut(s) 523
TfiI GAWTC 2 cut(s) 395, 489
Tru1I TTAA 1 cut(s) 482
Tru9I TTAA 1 cut(s) 482
TscAI CASTG 2 cut(s) 76, 285
TseFI GTSAC 1 cut(s) 506
TseI GCWGC 6 cut(s) 83, 137, 206, 246, 269, 453
Tsp45I GTSAC 1 cut(s) 506
TspDTI ATGAA 3 cut(s) 161, 197, 317
TspGWI ACGGA 1 cut(s) 249
TspRI CASTG 2 cut(s) 76, 285
VpaK11BI GGWCC 3 cut(s) 58, 95, 283
XcmI CCANNNNNNNNNTGG 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.