Rroxscaffold_7G00209840

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000645
Physical Location & Seq
Reverse (-)
60109208 .. 60109639
432 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00209840.1

Sequence Viewer

Length: 432 bp
ATGAAGACCATACTCTTTCTCCTCCTACTCTGCTACCTTTCGCTCACACTCCCCACTCTCTCCGATGCCACCCCAGCCTATCCTTCCGTTCCCGGTACCGCCCCCGCCGACTGTTCTTTCTCCGACGGCCATGAGGCCCATGACACTGCCCCTCTGATTCCCCCTCGCCGTGAAGTGTATGGAAATGGCCGAATCTTCGACATAACCCACCTGATCACCACGGCCACGCCATCGTATGGCTCCCCGGACGGCGTGCTGGGTCAGTTCCTGTGGTTCCCCCACAGAAACAAGAACGGCTCCATCGCGTCAGTTTTGAAGCTGCCTTCTCACACCGGCACGCATATTGATTTCCCTGGACACTACTTCCATCACTACCTCGATGCTGGCTTCGATGTCGATACGCTTGACTTGGAGGTCCTTAATGGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

143

Amino Acids

15.6

Weight (kDa)

5.57

Isoelectric Point (pI)

35.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 66 - 139 3.5e-06 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 413
Acc65I GGTACC 1 cut(s) 95
AccB1I GGYRCC 1 cut(s) 95
AccB7I CCANNNNNTGG 1 cut(s) 236
AccII CGCG 1 cut(s) 305
AciI CCGC 2 cut(s) 99, 105
AcoI YGGCCR 3 cut(s) 127, 187, 222
AfaI GTAC 1 cut(s) 97
AfiI CCNNNNNNNGG 1 cut(s) 236
AgsI TTSAA 1 cut(s) 316
AjnI CCWGG 1 cut(s) 352
AluBI AGCT 1 cut(s) 319
AluI AGCT 1 cut(s) 319
AlwNI CAGNNNCTG 1 cut(s) 268
AoxI GGCC 4 cut(s) 127, 135, 187, 222
ApeKI GCWGC 1 cut(s) 319
Asp718I GGTACC 1 cut(s) 95
AspS9I GGNCC 2 cut(s) 136, 415
AsuC2I CCSGG 2 cut(s) 93, 245
AsuHPI GGTGA 1 cut(s) 208
AvaII GGWCC 1 cut(s) 415
BanI GGYRCC 1 cut(s) 95
BbsI GAAGAC 1 cut(s) 11
BbvI GCAGC 1 cut(s) 306
BccI CCATC 3 cut(s) 238, 308, 375
BceAI ACGGC 5 cut(s) 142, 153, 237, 265, 310
BciT130I CCWGG 1 cut(s) 354
BclI TGATCA 1 cut(s) 213
BcnI CCSGG 2 cut(s) 93, 245
BisI GCNGC 1 cut(s) 320
BlsI GCNGC 1 cut(s) 321
Bme1390I CCNGG 3 cut(s) 93, 245, 354
Bme18I GGWCC 1 cut(s) 415
BmgT120I GGNCC 2 cut(s) 136, 415
BmiI GGNNCC 4 cut(s) 97, 241, 275, 298
BmrFI CCNGG 3 cut(s) 93, 245, 354
BmsI GCATC 2 cut(s) 55, 370
BpiI GAAGAC 1 cut(s) 11
BpuMI CCSGG 2 cut(s) 93, 245
BsaJI CCNNGG 3 cut(s) 219, 243, 352
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse118I RCCGGY 1 cut(s) 332
BseBI CCWGG 1 cut(s) 354
BseDI CCNNGG 3 cut(s) 219, 243, 352
BseLI CCNNNNNNNGG 1 cut(s) 236
BseRI GAGGAG 1 cut(s) 11
BseXI GCAGC 1 cut(s) 306
BseYI CCCAGC 2 cut(s) 73, 256
Bsh1236I CGCG 1 cut(s) 305
BshFI GGCC 4 cut(s) 129, 137, 189, 224
BshNI GGYRCC 1 cut(s) 95
BsiSI CCGG 3 cut(s) 93, 245, 333
BslI CCNNNNNNNGG 1 cut(s) 236
BsnI GGCC 4 cut(s) 129, 137, 189, 224
Bsp143I GATC 1 cut(s) 213
BspACI CCGC 2 cut(s) 99, 105
BspANI GGCC 4 cut(s) 129, 137, 189, 224
BspFNI CGCG 1 cut(s) 305
BspLI GGNNCC 4 cut(s) 97, 241, 275, 298
BspT107I GGYRCC 1 cut(s) 95
BsrFI RCCGGY 1 cut(s) 332
BssAI RCCGGY 1 cut(s) 332
BssECI CCNNGG 3 cut(s) 219, 243, 352
BssMI GATC 1 cut(s) 213
Bst2UI CCWGG 1 cut(s) 354
Bst4CI ACNGT 1 cut(s) 113
BstC8I GCNNGC 3 cut(s) 254, 338, 385
BstDSI CCRYGG 1 cut(s) 219
BstFNI CGCG 1 cut(s) 305
BstKTI GATC 1 cut(s) 216
BstMBI GATC 1 cut(s) 213
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstNI CCWGG 1 cut(s) 354
BstSCI CCNGG 3 cut(s) 91, 243, 352
BstUI CGCG 1 cut(s) 305
BstV1I GCAGC 1 cut(s) 306
BstV2I GAAGAC 1 cut(s) 11
BsuRI GGCC 4 cut(s) 129, 137, 189, 224
BtgI CCRYGG 1 cut(s) 219
BtgZI GCGATG 1 cut(s) 286
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 3 cut(s) 254, 338, 385
CaiI CAGNNNCTG 1 cut(s) 268
Cfr10I RCCGGY 1 cut(s) 332
Cfr13I GGNCC 2 cut(s) 136, 415
CseI GACGC 1 cut(s) 294
Csp6I GTAC 1 cut(s) 96
CviAII CATG 2 cut(s) 131, 140
CviJI RGCY 9 cut(s) 77, 129, 137, 189, 224, 240, 297, 319, 387
CviKI_1 RGCY 9 cut(s) 77, 129, 137, 189, 224, 240, 297, 319, 387
CviQI GTAC 1 cut(s) 96
DpnI GATC 1 cut(s) 215
DpnII GATC 1 cut(s) 213
DrdI GACNNNNNNGTC 1 cut(s) 413
DseDI GACNNNNNNGTC 1 cut(s) 413
EaeI YGGCCR 3 cut(s) 127, 187, 222
Eco47I GGWCC 1 cut(s) 415
EcoO109I RGGNCCY 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 352
FaeI CATG 2 cut(s) 134, 143
FaiI YATR 7 cut(s) 11, 132, 141, 180, 203, 237, 342
FatI CATG 2 cut(s) 130, 139
FauI CCCGC 1 cut(s) 112
FbaI TGATCA 1 cut(s) 213
Fnu4HI GCNGC 1 cut(s) 320
Fsp4HI GCNGC 1 cut(s) 320
GluI GCNGC 1 cut(s) 320
GsaI CCCAGC 2 cut(s) 77, 260
HaeIII GGCC 4 cut(s) 129, 137, 189, 224
HapII CCGG 3 cut(s) 93, 245, 333
HgaI GACGC 1 cut(s) 294
Hin1II CATG 2 cut(s) 134, 143
HinfI GANTC 2 cut(s) 157, 192
HpaII CCGG 3 cut(s) 93, 245, 333
HphI GGTGA 1 cut(s) 208
Hpy188I TCNGA 3 cut(s) 64, 124, 156
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 2 cut(s) 93, 333
HpyCH4III ACNGT 1 cut(s) 113
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
Hsp92II CATG 2 cut(s) 134, 143
KpnI GGTACC 1 cut(s) 99
Ksp22I TGATCA 1 cut(s) 213
Kzo9I GATC 1 cut(s) 213
LmnI GCTCC 2 cut(s) 245, 302
Lsp1109I GCAGC 1 cut(s) 306
LweI GCATC 2 cut(s) 55, 370
MalI GATC 1 cut(s) 215
MboI GATC 1 cut(s) 213
MboII GAAGA 2 cut(s) 16, 187
MluCI AATT 1 cut(s) 427
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 6 cut(s) 32, 127, 162, 174, 386, 406
MseI TTAA 1 cut(s) 420
MspI CCGG 3 cut(s) 93, 245, 333
MspR9I CCNGG 3 cut(s) 93, 245, 354
MvaI CCWGG 1 cut(s) 354
MvnI CGCG 1 cut(s) 305
MwoI GCNNNNNNNGC 1 cut(s) 74
NciI CCSGG 2 cut(s) 93, 245
NdeII GATC 1 cut(s) 213
NlaIII CATG 2 cut(s) 134, 143
NlaIV GGNNCC 4 cut(s) 97, 241, 275, 298
PfeI GAWTC 2 cut(s) 157, 192
PflMI CCANNNNNTGG 1 cut(s) 236
PkrI GCNGC 1 cut(s) 321
PpuMI RGGWCCY 1 cut(s) 415
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 1 cut(s) 352
PspFI CCCAGC 2 cut(s) 73, 256
PspGI CCWGG 1 cut(s) 352
PspN4I GGNNCC 4 cut(s) 97, 241, 275, 298
PspPI GGNCC 2 cut(s) 136, 415
PspPPI RGGWCCY 1 cut(s) 415
PstNI CAGNNNCTG 1 cut(s) 268
RsaI GTAC 1 cut(s) 97
RsaNI GTAC 1 cut(s) 96
SaqAI TTAA 1 cut(s) 420
SatI GCNGC 1 cut(s) 320
Sau3AI GATC 1 cut(s) 213
Sau96I GGNCC 2 cut(s) 136, 415
ScrFI CCNGG 3 cut(s) 93, 245, 354
SetI ASST 5 cut(s) 39, 213, 321, 378, 417
SfaNI GCATC 2 cut(s) 55, 370
SinI GGWCC 1 cut(s) 415
Sse9I AATT 1 cut(s) 427
SsiI CCGC 2 cut(s) 99, 105
StyD4I CCNGG 3 cut(s) 91, 243, 352
TaaI ACNGT 1 cut(s) 113
TaqI TCGA 4 cut(s) 198, 378, 390, 396
TasI AATT 1 cut(s) 427
TfiI GAWTC 2 cut(s) 157, 192
Tru1I TTAA 1 cut(s) 420
Tru9I TTAA 1 cut(s) 420
TscAI CASTG 1 cut(s) 151
TseI GCWGC 1 cut(s) 319
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 76
TspRI CASTG 1 cut(s) 151
Van91I CCANNNNNTGG 1 cut(s) 236
VpaK11BI GGWCC 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.