Rh6AG078600

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000645
Physical Location & Seq
Forward (+)
11433698 .. 11442498
8801 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG078600.1

Sequence Viewer

Length: 522 bp
ATGAATCCAATCCTTCTCCTTCTCCTCTTCTCCCTTCAGGCCACCCTCTCCCACGCCACCACAGCCTACCCTTCCATTCCGGGCACCACCCCCGTCGACGTCTCCTCCGGCGCCACCGCCACCAACCTGATCCCCCCTCGCCGCGAAGTCCACGGAAACGGCCGAATCTTCGACATCAGCCACCGCTACACCCCCGACATGCCGGCGTTTGGCTCCGCCGAGGGCGTCGGCCAGTTCCTCTGGCTCCCCAACAGCATGAAGAACGGCTCCATTGCTAACAACTCCGAGATGAAGCTCCCCACCCACACCGGGACCCACGTCGACGCCCCCGGCCACTTCTTCGATCACTACTTCGACGCCGGCTTCGACGTCGACACGCTCGACTTGGATGTCCTTAATGGTGCGGCATTGTTAGTTGACGTTCCGAGGGATAAGAACATTACCGCTGAAGTCATGGAGGGCTTACATATTCCCAAGGGGGTGAGTCGTGTACTTTTCAGAACTTTGAACACAGACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

173

Amino Acids

18.75

Weight (kDa)

5.66

Isoelectric Point (pI)

25.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 57 - 168 1.8e-13 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 389
AatII GACGTC 2 cut(s) 102, 372
AccB1I GGYRCC 2 cut(s) 83, 110
AccI GTMKAC 3 cut(s) 96, 321, 372
AccII CGCG 1 cut(s) 144
AciI CCGC 6 cut(s) 117, 142, 184, 216, 404, 444
AclWI GGATC 1 cut(s) 124
AcoI YGGCCR 3 cut(s) 160, 229, 331
AcuI CTGAAG 2 cut(s) 20, 468
AcyI GRCGYC 6 cut(s) 99, 111, 225, 324, 357, 369
AfaI GTAC 1 cut(s) 492
AfiI CCNNNNNNNGG 2 cut(s) 209, 309
AgsI TTSAA 1 cut(s) 508
AjiI CACGTC 1 cut(s) 319
AluBI AGCT 1 cut(s) 295
AluI AGCT 1 cut(s) 295
Alw26I GTCTC 1 cut(s) 106
AlwI GGATC 1 cut(s) 124
AoxI GGCC 4 cut(s) 39, 160, 229, 331
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 1 cut(s) 312
AsuC2I CCSGG 3 cut(s) 81, 310, 330
AsuHPI GGTGA 1 cut(s) 493
AvaII GGWCC 1 cut(s) 312
BaeGI GKGCMC 1 cut(s) 86
BanI GGYRCC 2 cut(s) 83, 110
BceAI ACGGC 2 cut(s) 175, 280
BcnI CCSGG 3 cut(s) 81, 310, 330
BcoDI GTCTC 1 cut(s) 106
BfoI RGCGCY 1 cut(s) 114
BisI GCNGC 2 cut(s) 142, 405
BlsI GCNGC 2 cut(s) 143, 406
Bme1390I CCNGG 3 cut(s) 81, 310, 330
Bme18I GGWCC 1 cut(s) 312
BmgBI CACGTC 1 cut(s) 319
BmgT120I GGNCC 1 cut(s) 312
BmiI GGNNCC 7 cut(s) 85, 112, 214, 245, 268, 313, 314
BmrFI CCNGG 3 cut(s) 81, 310, 330
BoxI GACNNNNGTC 1 cut(s) 317
BpuMI CCSGG 3 cut(s) 81, 310, 330
BsaHI GRCGYC 6 cut(s) 99, 111, 225, 324, 357, 369
BsaJI CCNNGG 5 cut(s) 151, 219, 328, 425, 474
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 2 cut(s) 209, 309
Bse118I RCCGGY 2 cut(s) 202, 359
Bse1I ACTGG 1 cut(s) 232
Bse3DI GCAATG 1 cut(s) 270
BseDI CCNNGG 5 cut(s) 151, 219, 328, 425, 474
BseGI GGATG 1 cut(s) 394
BseLI CCNNNNNNNGG 2 cut(s) 209, 309
BseMI GCAATG 1 cut(s) 270
BseNI ACTGG 1 cut(s) 232
BseRI GAGGAG 2 cut(s) 14, 94
BseSI GKGCMC 1 cut(s) 86
BseX3I CGGCCG 1 cut(s) 160
Bsh1236I CGCG 1 cut(s) 144
Bsh1285I CGRYCG 1 cut(s) 163
BshFI GGCC 4 cut(s) 41, 162, 231, 333
BshNI GGYRCC 2 cut(s) 83, 110
BsiEI CGRYCG 1 cut(s) 163
BsiSI CCGG 6 cut(s) 80, 108, 203, 309, 330, 360
BslFI GGGAC 1 cut(s) 325
BslI CCNNNNNNNGG 2 cut(s) 209, 309
BsmAI GTCTC 1 cut(s) 106
BsmBI CGTCTC 1 cut(s) 106
BsmFI GGGAC 1 cut(s) 325
BsnI GGCC 4 cut(s) 41, 162, 231, 333
Bsp1286I GDGCHC 1 cut(s) 86
Bsp143I GATC 2 cut(s) 129, 343
BspACI CCGC 6 cut(s) 117, 142, 184, 216, 404, 444
BspANI GGCC 4 cut(s) 41, 162, 231, 333
BspFNI CGCG 1 cut(s) 144
BspLI GGNNCC 7 cut(s) 85, 112, 214, 245, 268, 313, 314
BspPI GGATC 1 cut(s) 124
BspT107I GGYRCC 2 cut(s) 83, 110
BsrDI GCAATG 1 cut(s) 270
BsrFI RCCGGY 2 cut(s) 202, 359
BsrI ACTGG 1 cut(s) 232
BssAI RCCGGY 2 cut(s) 202, 359
BssECI CCNNGG 5 cut(s) 151, 219, 328, 425, 474
BssMI GATC 2 cut(s) 129, 343
BssNI GRCGYC 6 cut(s) 99, 111, 225, 324, 357, 369
BssT1I CCWWGG 1 cut(s) 474
Bst6I CTCTTC 1 cut(s) 32
BstACI GRCGYC 6 cut(s) 99, 111, 225, 324, 357, 369
BstC8I GCNNGC 2 cut(s) 204, 361
BstDSI CCRYGG 1 cut(s) 151
BstF5I GGATG 1 cut(s) 394
BstFNI CGCG 1 cut(s) 144
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 1 cut(s) 113
BstKTI GATC 2 cut(s) 132, 346
BstMAI GTCTC 1 cut(s) 106
BstMBI GATC 2 cut(s) 129, 343
BstMCI CGRYCG 1 cut(s) 163
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNSI RCATGY 1 cut(s) 202
BstPAI GACNNNNGTC 1 cut(s) 317
BstSCI CCNGG 3 cut(s) 79, 308, 328
BstSLI GKGCMC 1 cut(s) 86
BstUI CGCG 1 cut(s) 144
BstZI CGGCCG 1 cut(s) 160
BsuRI GGCC 4 cut(s) 41, 162, 231, 333
BtgI CCRYGG 1 cut(s) 151
BtrI CACGTC 1 cut(s) 319
BtsCI GGATG 1 cut(s) 394
Cac8I GCNNGC 2 cut(s) 204, 361
CfoI GCGC 1 cut(s) 113
Cfr10I RCCGGY 2 cut(s) 202, 359
Cfr13I GGNCC 1 cut(s) 312
CseI GACGC 3 cut(s) 214, 332, 365
Csp6I GTAC 1 cut(s) 491
CviAII CATG 3 cut(s) 199, 256, 454
CviQI GTAC 1 cut(s) 491
DinI GGCGCC 1 cut(s) 112
DpnI GATC 2 cut(s) 131, 345
DpnII GATC 2 cut(s) 129, 343
DrdI GACNNNNNNGTC 1 cut(s) 389
DseDI GACNNNNNNGTC 1 cut(s) 389
EaeI YGGCCR 3 cut(s) 160, 229, 331
EagI CGGCCG 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EciI GGCGGA 1 cut(s) 205
EclXI CGGCCG 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 474
Eco47I GGWCC 1 cut(s) 312
Eco52I CGGCCG 1 cut(s) 160
Eco57I CTGAAG 2 cut(s) 20, 468
EcoO109I RGGNCCY 1 cut(s) 312
EcoT14I CCWWGG 1 cut(s) 474
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 474
Esp3I CGTCTC 1 cut(s) 106
FaeI CATG 3 cut(s) 202, 259, 457
FaiI YATR 4 cut(s) 200, 257, 455, 468
FaqI GGGAC 1 cut(s) 325
FatI CATG 3 cut(s) 198, 255, 453
FblI GTMKAC 3 cut(s) 96, 321, 372
Fnu4HI GCNGC 2 cut(s) 142, 405
FokI GGATG 1 cut(s) 401
Fsp4HI GCNGC 2 cut(s) 142, 405
GlaI GCGC 1 cut(s) 112
GluI GCNGC 2 cut(s) 142, 405
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 4 cut(s) 41, 162, 231, 333
HapII CCGG 6 cut(s) 80, 108, 203, 309, 330, 360
HgaI GACGC 3 cut(s) 214, 332, 365
HhaI GCGC 1 cut(s) 113
Hin1I GRCGYC 6 cut(s) 99, 111, 225, 324, 357, 369
Hin1II CATG 3 cut(s) 202, 259, 457
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HincII GTYRAC 4 cut(s) 97, 322, 373, 418
HindII GTYRAC 4 cut(s) 97, 322, 373, 418
HinfI GANTC 3 cut(s) 4, 165, 484
HpaII CCGG 6 cut(s) 80, 108, 203, 309, 330, 360
HphI GGTGA 1 cut(s) 493
Hpy166II GTNNAC 6 cut(s) 97, 151, 322, 373, 418, 491
Hpy188I TCNGA 3 cut(s) 286, 426, 500
Hpy8I GTNNAC 6 cut(s) 97, 151, 322, 373, 418, 491
Hpy99I CGWCG 8 cut(s) 98, 101, 230, 323, 326, 359, 371, 374
HpyAV CCTTC 4 cut(s) 23, 29, 44, 81
HpyCH4IV ACGT 4 cut(s) 99, 318, 369, 420
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpySE526I ACGT 4 cut(s) 99, 318, 369, 420
Hsp92I GRCGYC 6 cut(s) 99, 111, 225, 324, 357, 369
Hsp92II CATG 3 cut(s) 202, 259, 457
HspAI GCGC 1 cut(s) 111
KasI GGCGCC 1 cut(s) 110
KflI GGGWCCC 1 cut(s) 312
KroI GCCGGC 2 cut(s) 202, 359
KroNI GCCGGC 2 cut(s) 204, 361
Kzo9I GATC 2 cut(s) 129, 343
LmnI GCTCC 4 cut(s) 218, 249, 272, 300
MaeII ACGT 4 cut(s) 99, 318, 369, 420
MalI GATC 2 cut(s) 131, 345
MboI GATC 2 cut(s) 129, 343
MboII GAAGA 4 cut(s) 19, 160, 271, 331
MhlI GDGCHC 1 cut(s) 86
Mly113I GGCGCC 1 cut(s) 111
MlyI GAGTC 1 cut(s) 493
MnlI CCTC 8 cut(s) 35, 56, 115, 147, 214, 248, 420, 451
MroNI GCCGGC 2 cut(s) 202, 359
MseI TTAA 1 cut(s) 396
MspA1I CMGCKG 1 cut(s) 446
MspI CCGG 6 cut(s) 80, 108, 203, 309, 330, 360
MspR9I CCNGG 3 cut(s) 81, 310, 330
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 1 cut(s) 62
NaeI GCCGGC 2 cut(s) 204, 361
NarI GGCGCC 1 cut(s) 111
NciI CCSGG 3 cut(s) 81, 310, 330
NdeII GATC 2 cut(s) 129, 343
NgoMIV GCCGGC 2 cut(s) 202, 359
NlaIII CATG 3 cut(s) 202, 259, 457
NlaIV GGNNCC 7 cut(s) 85, 112, 214, 245, 268, 313, 314
NmeAIII GCCGAG 1 cut(s) 244
NspI RCATGY 1 cut(s) 202
PcsI WCGNNNNNNNCGW 2 cut(s) 363, 378
PdiI GCCGGC 2 cut(s) 204, 361
PfeI GAWTC 2 cut(s) 4, 165
PkrI GCNGC 2 cut(s) 143, 406
PleI GAGTC 1 cut(s) 492
PluTI GGCGCC 1 cut(s) 114
PpsI GAGTC 1 cut(s) 492
PpuMI RGGWCCY 1 cut(s) 312
PshAI GACNNNNGTC 1 cut(s) 317
Psp5II RGGWCCY 1 cut(s) 312
PspN4I GGNNCC 7 cut(s) 85, 112, 214, 245, 268, 313, 314
PspPI GGNCC 1 cut(s) 312
PspPPI RGGWCCY 1 cut(s) 312
RsaI GTAC 1 cut(s) 492
RsaNI GTAC 1 cut(s) 491
SalI GTCGAC 3 cut(s) 95, 320, 371
SaqAI TTAA 1 cut(s) 396
SatI GCNGC 2 cut(s) 142, 405
Sau3AI GATC 2 cut(s) 129, 343
Sau96I GGNCC 1 cut(s) 312
SchI GAGTC 1 cut(s) 493
ScrFI CCNGG 3 cut(s) 81, 310, 330
SduI GDGCHC 1 cut(s) 86
SetI ASST 6 cut(s) 102, 129, 297, 321, 372, 423
SfoI GGCGCC 1 cut(s) 112
SgrDI CGTCGACG 2 cut(s) 95, 320
SinI GGWCC 1 cut(s) 312
SsiI CCGC 6 cut(s) 117, 142, 184, 216, 404, 444
SspDI GGCGCC 1 cut(s) 110
StyD4I CCNGG 3 cut(s) 79, 308, 328
StyI CCWWGG 1 cut(s) 474
TaiI ACGT 4 cut(s) 102, 321, 372, 423
TaqI TCGA 8 cut(s) 96, 171, 321, 342, 354, 366, 372, 381
TatI WGTACW 1 cut(s) 490
TauI GCSGC 2 cut(s) 144, 407
TfiI GAWTC 2 cut(s) 4, 165
Tru1I TTAA 1 cut(s) 396
Tru9I TTAA 1 cut(s) 396
TspDTI ATGAA 3 cut(s) 17, 272, 305
TspGWI ACGGA 1 cut(s) 168
VpaK11BI GGWCC 1 cut(s) 312
XceI RCATGY 1 cut(s) 202
XmiI GTMKAC 3 cut(s) 96, 321, 372
ZraI GACGTC 2 cut(s) 100, 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.