Rh4BG250600

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000645
Physical Location & Seq
Forward (+)
41980432 .. 41980584
153 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG250600.1

Sequence Viewer

Length: 153 bp
ATGCCGGCGTTTGACTCCGTCGATGGCGTCGGCCAGTTCCTCTGGCTCCCCAACAGCATGAAGAACGGACCCATTGCTAACAACTCCAAGATGAAGCTCCCTACCCACACCGGAACCCATGTCGACGCCCCCGGCCACGTCTTCGATCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

50

Amino Acids

5.42

Weight (kDa)

6.36

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 19 - 48 7.7e-06 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 123
AcoI YGGCCR 2 cut(s) 31, 133
AcyI GRCGYC 2 cut(s) 27, 126
AjiI CACGTC 1 cut(s) 139
AluBI AGCT 1 cut(s) 97
AluI AGCT 1 cut(s) 97
AoxI GGCC 2 cut(s) 31, 133
AspS9I GGNCC 1 cut(s) 68
AsuC2I CCSGG 1 cut(s) 132
AvaII GGWCC 1 cut(s) 68
BbsI GAAGAC 1 cut(s) 133
BccI CCATC 1 cut(s) 17
BcnI CCSGG 1 cut(s) 132
BfaI CTAG 1 cut(s) 151
Bme1390I CCNGG 1 cut(s) 132
Bme18I GGWCC 1 cut(s) 68
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 68
BmiI GGNNCC 3 cut(s) 47, 70, 115
BmrFI CCNGG 1 cut(s) 132
BpiI GAAGAC 1 cut(s) 133
BpuMI CCSGG 1 cut(s) 132
BsaHI GRCGYC 2 cut(s) 27, 126
BsaJI CCNNGG 1 cut(s) 130
BsaWI WCCGGW 1 cut(s) 110
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 1 cut(s) 34
Bse3DI GCAATG 1 cut(s) 72
BseDI CCNNGG 1 cut(s) 130
BseMI GCAATG 1 cut(s) 72
BseNI ACTGG 1 cut(s) 34
BshFI GGCC 2 cut(s) 33, 135
BsiSI CCGG 3 cut(s) 5, 111, 132
BsnI GGCC 2 cut(s) 33, 135
Bsp143I GATC 1 cut(s) 145
BspANI GGCC 2 cut(s) 33, 135
BspLI GGNNCC 3 cut(s) 47, 70, 115
BsrDI GCAATG 1 cut(s) 72
BsrFI RCCGGY 1 cut(s) 4
BsrI ACTGG 1 cut(s) 34
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 1 cut(s) 130
BssMI GATC 1 cut(s) 145
BssNI GRCGYC 2 cut(s) 27, 126
BstACI GRCGYC 2 cut(s) 27, 126
BstC8I GCNNGC 1 cut(s) 6
BstKTI GATC 1 cut(s) 148
BstMBI GATC 1 cut(s) 145
BstSCI CCNGG 1 cut(s) 130
BstV2I GAAGAC 1 cut(s) 133
BsuRI GGCC 2 cut(s) 33, 135
BtrI CACGTC 1 cut(s) 139
Cac8I GCNNGC 1 cut(s) 6
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 1 cut(s) 68
CseI GACGC 2 cut(s) 16, 134
CviAII CATG 2 cut(s) 58, 119
CviJI RGCY 4 cut(s) 33, 46, 97, 135
CviKI_1 RGCY 4 cut(s) 33, 46, 97, 135
DpnI GATC 1 cut(s) 147
DpnII GATC 1 cut(s) 145
EaeI YGGCCR 2 cut(s) 31, 133
Eco47I GGWCC 1 cut(s) 68
FaeI CATG 2 cut(s) 61, 122
FaiI YATR 2 cut(s) 59, 120
FatI CATG 2 cut(s) 57, 118
FblI GTMKAC 1 cut(s) 123
FspBI CTAG 1 cut(s) 151
HaeIII GGCC 2 cut(s) 33, 135
HapII CCGG 3 cut(s) 5, 111, 132
HgaI GACGC 2 cut(s) 16, 134
Hin1I GRCGYC 2 cut(s) 27, 126
Hin1II CATG 2 cut(s) 61, 122
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 1 cut(s) 14
HpaII CCGG 3 cut(s) 5, 111, 132
Hpy166II GTNNAC 1 cut(s) 124
Hpy8I GTNNAC 1 cut(s) 124
Hpy99I CGWCG 3 cut(s) 23, 32, 128
HpyCH4IV ACGT 1 cut(s) 138
HpySE526I ACGT 1 cut(s) 138
Hsp92I GRCGYC 2 cut(s) 27, 126
Hsp92II CATG 2 cut(s) 61, 122
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Kzo9I GATC 1 cut(s) 145
LmnI GCTCC 2 cut(s) 51, 102
LpnPI CCDG 5 cut(s) 18, 28, 47, 124, 145
MaeI CTAG 1 cut(s) 151
MaeII ACGT 1 cut(s) 138
MalI GATC 1 cut(s) 147
MboI GATC 1 cut(s) 145
MboII GAAGA 2 cut(s) 73, 133
MlyI GAGTC 1 cut(s) 8
MnlI CCTC 1 cut(s) 50
MroNI GCCGGC 1 cut(s) 4
MspI CCGG 3 cut(s) 5, 111, 132
MspR9I CCNGG 1 cut(s) 132
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 1 cut(s) 132
NdeII GATC 1 cut(s) 145
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 2 cut(s) 61, 122
NlaIV GGNNCC 3 cut(s) 47, 70, 115
PdiI GCCGGC 1 cut(s) 6
PflFI GACNNNGTC 1 cut(s) 17
PleI GAGTC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 8
PspN4I GGNNCC 3 cut(s) 47, 70, 115
PspPI GGNCC 1 cut(s) 68
PsyI GACNNNGTC 1 cut(s) 17
SalI GTCGAC 1 cut(s) 122
Sau3AI GATC 1 cut(s) 145
Sau96I GGNCC 1 cut(s) 68
SchI GAGTC 1 cut(s) 8
ScrFI CCNGG 1 cut(s) 132
SetI ASST 2 cut(s) 99, 141
SinI GGWCC 1 cut(s) 68
SspMI CTAG 1 cut(s) 151
StyD4I CCNGG 1 cut(s) 130
TaiI ACGT 1 cut(s) 141
TaqI TCGA 3 cut(s) 21, 123, 144
TspDTI ATGAA 2 cut(s) 74, 107
TspGWI ACGGA 2 cut(s) 7, 81
Tth111I GACNNNGTC 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 68
XmiI GTMKAC 1 cut(s) 123
XspI CTAG 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.