Rh6CG069700

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000645
Physical Location & Seq
Reverse (-)
6895582 .. 6897432
1851 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG069700.1

Sequence Viewer

Length: 825 bp
ATGTGCACGGCCGTTGCCACCGTCACCTCATCCTCTGACTCAGCTTACCCCACAGTCACCGAGGACTGCTCGGTGGGCTCCGGCGAGGCCGTCCCTGTCCCGGTACGTAGAGAGGTGTACGATAACGGTCGAATATTCGACATCAGCCACAGGTACACTGCTGAGATGCCGAGTTTTGCCTCCGAAAACGGGCGACTGGGAAGCGACTTCTTGTGGCTGGGATTCAGCATCAAGAACGGATCGCTGGCAAATGCCTCCATGATGAAGTTCTGTGTCCACTCGGGCACTCACGTCGACGCACCTGGTCACGTCTTCGACCATTACTACCATGCTGGATTTGATGTCGATGCTTTAGACCTCGAAGTGTTAAACGGCCCTGCGCTGGTGGTTGATGTTCCCAGGGATAAGAACACAACTGTTGAAGTAATGAAGGCTTTGAACATTCCTAAAGGAGTGCGTCGTGTGCTTTTCAGAACCTTAAATACTGACAGAACCTGGATATTAAATTATTTGCTTACTACATTCAAGAAAATTATGTGGAACAAAGAGTACGACACGAGCGCCACAGGATTTACGGAGGATGGGGCAAAATGGTTAGTTCAAAACACAGACATCAAACTCGTTGGAATTGATTACTTAAACGTTGCAGCTTTCGTTGATGTAGTTCCAGTTCATAATACTTTTCTGGCAGGCAGGGAGATAATTCTTGTCGAAGGTTTAAAGCTCGAAGGAGTTCCGGCGGGATTATATTCTTCTGTTCATTGCTTGCCTTTAAGGTTGATTGGTTCCGATGGATCACCAGCAAGATGCATTCTCATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

274

Amino Acids

30.06

Weight (kDa)

5.74

Isoelectric Point (pI)

36.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 46 - 215 6.3e-20 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 294
AciI CCGC 1 cut(s) 740
AclI AACGTT 1 cut(s) 642
AclWI GGATC 2 cut(s) 247, 802
AcoI YGGCCR 1 cut(s) 9
AfaI GTAC 4 cut(s) 105, 119, 155, 551
AfiI CCNNNNNNNGG 3 cut(s) 100, 189, 382
AgsI TTSAA 4 cut(s) 422, 439, 526, 602
AjiI CACGTC 2 cut(s) 292, 310
AjnI CCWGG 3 cut(s) 301, 398, 494
AluBI AGCT 3 cut(s) 44, 650, 724
AluI AGCT 3 cut(s) 44, 650, 724
Alw21I GWGCWC 1 cut(s) 8
Alw44I GTGCAC 1 cut(s) 4
AlwI GGATC 2 cut(s) 247, 802
AlwNI CAGNNNCTG 1 cut(s) 495
Ama87I CYCGRG 1 cut(s) 280
AoxI GGCC 3 cut(s) 9, 87, 373
ApaLI GTGCAC 1 cut(s) 4
ApeKI GCWGC 1 cut(s) 647
Asp700I GAANNNNTTC 1 cut(s) 732
AspLEI GCGC 2 cut(s) 382, 563
AspS9I GGNCC 1 cut(s) 374
AsuC2I CCSGG 1 cut(s) 101
AsuHPI GGTGA 3 cut(s) 16, 49, 789
AvaI CYCGRG 1 cut(s) 280
BaeGI GKGCMC 2 cut(s) 8, 287
BanII GRGCYC 1 cut(s) 80
BauI CACGAG 1 cut(s) 556
BbsI GAAGAC 1 cut(s) 304
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 1 cut(s) 659
BccI CCATC 2 cut(s) 575, 785
BceAI ACGGC 3 cut(s) 24, 74, 388
BcgI CGANNNNNNTGC 2 cut(s) 274, 308
BciT130I CCWGG 3 cut(s) 303, 400, 496
BcnI CCSGG 1 cut(s) 101
BfoI RGCGCY 1 cut(s) 564
BisI GCNGC 1 cut(s) 648
BlsI GCNGC 1 cut(s) 649
Bme1390I CCNGG 4 cut(s) 101, 303, 400, 496
BmeT110I CYCGRG 1 cut(s) 280
BmgBI CACGTC 2 cut(s) 292, 310
BmgT120I GGNCC 1 cut(s) 374
BmiI GGNNCC 2 cut(s) 79, 787
BmrFI CCNGG 4 cut(s) 101, 303, 400, 496
BmrI ACTGGG 1 cut(s) 206
BmsI GCATC 4 cut(s) 156, 237, 337, 797
BmuI ACTGGG 1 cut(s) 206
BpiI GAAGAC 1 cut(s) 304
BplI GAGNNNNNCTC 2 cut(s) 53, 85
BpuMI CCSGG 1 cut(s) 101
BsaAI YACGTR 1 cut(s) 107
BsaJI CCNNGG 3 cut(s) 60, 398, 399
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 189, 382
Bse1I ACTGG 2 cut(s) 201, 668
Bse3DI GCAATG 1 cut(s) 760
BseBI CCWGG 3 cut(s) 303, 400, 496
BseDI CCNNGG 3 cut(s) 60, 398, 399
BseGI GGATG 2 cut(s) 29, 586
BseLI CCNNNNNNNGG 3 cut(s) 100, 189, 382
BseMI GCAATG 1 cut(s) 760
BseMII CTCAG 2 cut(s) 54, 153
BseNI ACTGG 2 cut(s) 201, 668
BseSI GKGCMC 2 cut(s) 8, 287
BseX3I CGGCCG 1 cut(s) 9
BseXI GCAGC 1 cut(s) 659
BseYI CCCAGC 1 cut(s) 217
Bsh1285I CGRYCG 2 cut(s) 12, 130
BshFI GGCC 3 cut(s) 11, 89, 375
BsiEI CGRYCG 2 cut(s) 12, 130
BsiHKAI GWGCWC 1 cut(s) 8
BsiHKCI CYCGRG 1 cut(s) 280
BsiSI CCGG 3 cut(s) 81, 101, 737
BslFI GGGAC 2 cut(s) 77, 83
BslI CCNNNNNNNGG 3 cut(s) 100, 189, 382
BsmFI GGGAC 2 cut(s) 77, 83
BsmI GAATGC 1 cut(s) 810
BsnI GGCC 3 cut(s) 11, 89, 375
BsoBI CYCGRG 1 cut(s) 280
Bsp1286I GDGCHC 3 cut(s) 8, 80, 287
Bsp143I GATC 2 cut(s) 239, 794
BspACI CCGC 1 cut(s) 740
BspANI GGCC 3 cut(s) 11, 89, 375
BspCNI CTCAG 2 cut(s) 53, 154
BspLI GGNNCC 2 cut(s) 79, 787
BspPI GGATC 2 cut(s) 247, 802
BsrDI GCAATG 1 cut(s) 760
BsrI ACTGG 2 cut(s) 201, 668
BssECI CCNNGG 3 cut(s) 60, 398, 399
BssMI GATC 2 cut(s) 239, 794
BssSI CACGAG 1 cut(s) 556
Bst2BI CACGAG 1 cut(s) 556
Bst2UI CCWGG 3 cut(s) 303, 400, 496
Bst4CI ACNGT 4 cut(s) 22, 55, 128, 418
BstBAI YACGTR 1 cut(s) 107
BstC8I GCNNGC 3 cut(s) 246, 691, 767
BstDEI CTNAG 2 cut(s) 40, 162
BstF5I GGATG 2 cut(s) 29, 586
BstH2I RGCGCY 1 cut(s) 564
BstHHI GCGC 2 cut(s) 382, 563
BstKTI GATC 2 cut(s) 242, 797
BstMBI GATC 2 cut(s) 239, 794
BstMCI CGRYCG 2 cut(s) 12, 130
BstMWI GCNNNNNNNGC 2 cut(s) 75, 463
BstNI CCWGG 3 cut(s) 303, 400, 496
BstSCI CCNGG 4 cut(s) 99, 301, 398, 494
BstSLI GKGCMC 2 cut(s) 8, 287
BstSNI TACGTA 1 cut(s) 107
BstV1I GCAGC 1 cut(s) 659
BstV2I GAAGAC 1 cut(s) 304
BstZI CGGCCG 1 cut(s) 9
BsuRI GGCC 3 cut(s) 11, 89, 375
BtrI CACGTC 2 cut(s) 292, 310
BtsCI GGATG 2 cut(s) 29, 586
BtsI GCAGTG 1 cut(s) 156
BtsIMutI CAGTG 1 cut(s) 156
Cac8I GCNNGC 3 cut(s) 246, 691, 767
CaiI CAGNNNCTG 1 cut(s) 495
CfoI GCGC 2 cut(s) 382, 563
Cfr13I GGNCC 1 cut(s) 374
CseI GACGC 2 cut(s) 305, 446
CsiI ACCWGGT 1 cut(s) 301
Csp6I GTAC 4 cut(s) 104, 118, 154, 550
CviAII CATG 2 cut(s) 259, 329
CviQI GTAC 4 cut(s) 104, 118, 154, 550
DdeI CTNAG 2 cut(s) 40, 162
DpnI GATC 2 cut(s) 241, 796
DpnII GATC 2 cut(s) 239, 794
DraI TTTAAA 1 cut(s) 720
EaeI YGGCCR 1 cut(s) 9
EagI CGGCCG 1 cut(s) 9
EclXI CGGCCG 1 cut(s) 9
Eco105I TACGTA 1 cut(s) 107
Eco24I GRGCYC 1 cut(s) 80
Eco52I CGGCCG 1 cut(s) 9
Eco88I CYCGRG 1 cut(s) 280
EcoRII CCWGG 3 cut(s) 301, 398, 494
EcoT22I ATGCAT 1 cut(s) 812
EcoT38I GRGCYC 1 cut(s) 80
FaeI CATG 2 cut(s) 262, 332
FaiI YATR 5 cut(s) 260, 330, 536, 675, 748
FaqI GGGAC 2 cut(s) 77, 83
FatI CATG 2 cut(s) 258, 328
FauI CCCGC 1 cut(s) 733
FblI GTMKAC 1 cut(s) 294
Fnu4HI GCNGC 1 cut(s) 648
FokI GGATG 2 cut(s) 16, 593
FriOI GRGCYC 1 cut(s) 80
Fsp4HI GCNGC 1 cut(s) 648
GlaI GCGC 2 cut(s) 381, 562
GluI GCNGC 1 cut(s) 648
GsaI CCCAGC 1 cut(s) 221
HaeII RGCGCY 1 cut(s) 564
HaeIII GGCC 3 cut(s) 11, 89, 375
HapII CCGG 3 cut(s) 81, 101, 737
HgaI GACGC 2 cut(s) 305, 446
HhaI GCGC 2 cut(s) 382, 563
Hin1II CATG 2 cut(s) 262, 332
Hin6I GCGC 2 cut(s) 380, 561
HinP1I GCGC 2 cut(s) 380, 561
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 2 cut(s) 38, 222
HpaII CCGG 3 cut(s) 81, 101, 737
HphI GGTGA 3 cut(s) 16, 49, 789
Hpy166II GTNNAC 5 cut(s) 6, 118, 156, 277, 295
Hpy188I TCNGA 4 cut(s) 37, 184, 473, 790
Hpy188III TCNNGA 2 cut(s) 232, 526
Hpy8I GTNNAC 5 cut(s) 6, 118, 156, 277, 295
Hpy99I CGWCG 3 cut(s) 296, 299, 462
HpyAV CCTTC 3 cut(s) 424, 707, 722
HpyCH4III ACNGT 4 cut(s) 22, 55, 128, 418
HpyCH4IV ACGT 4 cut(s) 106, 291, 309, 642
HpyCH4V TGCA 3 cut(s) 6, 647, 810
HpyF10VI GCNNNNNNNGC 2 cut(s) 75, 463
HpyF3I CTNAG 2 cut(s) 40, 162
HpySE526I ACGT 4 cut(s) 106, 291, 309, 642
Hsp92II CATG 2 cut(s) 262, 332
HspAI GCGC 2 cut(s) 380, 561
Kzo9I GATC 2 cut(s) 239, 794
LmnI GCTCC 1 cut(s) 83
Lsp1109I GCAGC 1 cut(s) 659
LweI GCATC 4 cut(s) 156, 237, 337, 797
MabI ACCWGGT 1 cut(s) 301
MaeII ACGT 4 cut(s) 106, 291, 309, 642
MaeIII GTNAC 3 cut(s) 22, 55, 305
MalI GATC 2 cut(s) 241, 796
MboI GATC 2 cut(s) 239, 794
MboII GAAGA 2 cut(s) 304, 744
MhlI GDGCHC 3 cut(s) 8, 80, 287
MluCI AATT 4 cut(s) 505, 531, 627, 702
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 604
MnlI CCTC 9 cut(s) 37, 43, 55, 79, 106, 190, 265, 368, 571
Mph1103I ATGCAT 1 cut(s) 812
MroXI GAANNNNTTC 1 cut(s) 732
MseI TTAA 6 cut(s) 368, 479, 503, 638, 719, 773
MspI CCGG 3 cut(s) 81, 101, 737
MspR9I CCNGG 4 cut(s) 101, 303, 400, 496
Mva1269I GAATGC 1 cut(s) 810
MvaI CCWGG 3 cut(s) 303, 400, 496
MwoI GCNNNNNNNGC 2 cut(s) 75, 463
NciI CCSGG 1 cut(s) 101
NdeII GATC 2 cut(s) 239, 794
NlaIII CATG 2 cut(s) 262, 332
NlaIV GGNNCC 2 cut(s) 79, 787
NmeAIII GCCGAG 1 cut(s) 195
NmuCI GTSAC 3 cut(s) 22, 55, 305
NsiI ATGCAT 1 cut(s) 812
PasI CCCWGGG 1 cut(s) 399
PctI GAATGC 1 cut(s) 810
PdmI GAANNNNTTC 1 cut(s) 732
PfeI GAWTC 1 cut(s) 222
PkrI GCNGC 1 cut(s) 649
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
Ppu21I YACGTR 1 cut(s) 107
Psp1406I AACGTT 1 cut(s) 642
Psp6I CCWGG 3 cut(s) 301, 398, 494
PspFI CCCAGC 1 cut(s) 217
PspGI CCWGG 3 cut(s) 301, 398, 494
PspN4I GGNNCC 2 cut(s) 79, 787
PspPI GGNCC 1 cut(s) 374
PsrI GAACNNNNNNTAC 6 cut(s) 466, 498, 533, 565, 654, 686
PstNI CAGNNNCTG 1 cut(s) 495
RsaI GTAC 4 cut(s) 105, 119, 155, 551
RsaNI GTAC 4 cut(s) 104, 118, 154, 550
SalI GTCGAC 1 cut(s) 293
SaqAI TTAA 6 cut(s) 368, 479, 503, 638, 719, 773
SatI GCNGC 1 cut(s) 648
Sau3AI GATC 2 cut(s) 239, 794
Sau96I GGNCC 1 cut(s) 374
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 4 cut(s) 101, 303, 400, 496
SduI GDGCHC 3 cut(s) 8, 80, 287
SexAI ACCWGGT 1 cut(s) 301
SfaNI GCATC 4 cut(s) 156, 237, 337, 797
SgrDI CGTCGACG 1 cut(s) 293
SnaBI TACGTA 1 cut(s) 107
Sse9I AATT 4 cut(s) 505, 531, 627, 702
SsiI CCGC 1 cut(s) 740
SspI AATATT 1 cut(s) 135
StyD4I CCNGG 4 cut(s) 99, 301, 398, 494
TaaI ACNGT 4 cut(s) 22, 55, 128, 418
TaiI ACGT 4 cut(s) 109, 294, 312, 645
TaqI TCGA 8 cut(s) 130, 138, 294, 315, 345, 360, 711, 726
TasI AATT 4 cut(s) 505, 531, 627, 702
TfiI GAWTC 1 cut(s) 222
Tru1I TTAA 6 cut(s) 368, 479, 503, 638, 719, 773
Tru9I TTAA 6 cut(s) 368, 479, 503, 638, 719, 773
TscAI CASTG 1 cut(s) 163
TseFI GTSAC 3 cut(s) 22, 55, 305
TseI GCWGC 1 cut(s) 647
Tsp45I GTSAC 3 cut(s) 22, 55, 305
TspDTI ATGAA 4 cut(s) 278, 443, 662, 749
TspGWI ACGGA 2 cut(s) 252, 590
TspRI CASTG 1 cut(s) 163
VneI GTGCAC 1 cut(s) 4
XmiI GTMKAC 1 cut(s) 294
XmnI GAANNNNTTC 1 cut(s) 732
Zsp2I ATGCAT 1 cut(s) 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.