pycom05g08710

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000645
Physical Location & Seq
Reverse (-)
11468868 .. 11471743
2876 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g08710.5

Sequence Viewer

Length: 804 bp
ATGAAGCCAATACTTCTCCTCCTCCTACTCTCCTCCCTCTCCTACTCCGTCGCCACCACAGCCTATCCTTCTATTCCGGGCACCATCTCCATCGAATACGCCGCCGAAACCGACACCCTGATCCCTCCTCGCCGTGAAGTTTACGGTAATGGACGAATCTTCGACATCAGCCACCGGTACAGCACCACCATGCCTGCCTTTGAAACCAACGACGGTGTCGGCCAGTTCCTTTGGCTCCCCAACAGCATGAAGAACGGCTCCATCGCCAACAACTCTGAGATGAAGCTGCCCACTCACACTGGTACCCACGTCGATGCCCCGGGCCACGTCTTCGATCACTACTTCGACGCCGGCTTCGATGTCGATACCCTTGACTTGGATGTGCTTAATGGTCCTGCATTGCTAGTTGACGTTCCGAGGGAGAAGAACATAACCGCTGAGGTTATGAAGTCCTTACATATTCCCAAGGGGGTACGTCGTGTGCTGTTCAGAACATTAAACACAGACAGACGGCTTATGTTCCAAAAGGAGTTTGACAGCAGCTATGTGGGATTTATGAGGGATGGAGCACAGTGGTTGGTGGAGAACACTGACATTAAACTTGTCGGAATTGATTACTTATCCGTTGCTGCATATGATGATTTGATTCCATCCCACCTTGTTTTTCTAGAAGGCAGGGAAATCATTCTTGTTGAAGCCTTAAAACTGGATGACGTCGAACCAGGAATATATTCTGTCCACTGCTTACCTTTGAGGTTGCCGGGTGCAGAAGGGTCACCGATAAGATGCATTCTTATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

268

Amino Acids

29.76

Weight (kDa)

5.23

Isoelectric Point (pI)

33.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 717
Acc65I GGTACC 1 cut(s) 302
AccB1I GGYRCC 2 cut(s) 80, 302
AciI CCGC 2 cut(s) 102, 435
AclWI GGATC 1 cut(s) 115
AcoI YGGCCR 1 cut(s) 220
AcyI GRCGYC 2 cut(s) 348, 714
AfaI GTAC 3 cut(s) 179, 304, 474
AfiI CCNNNNNNNGG 1 cut(s) 376
AgeI ACCGGT 1 cut(s) 174
AgsI TTSAA 2 cut(s) 203, 695
AjiI CACGTC 2 cut(s) 310, 328
AjnI CCWGG 1 cut(s) 721
AluBI AGCT 2 cut(s) 286, 543
AluI AGCT 2 cut(s) 286, 543
Alw21I GWGCWC 1 cut(s) 571
AlwI GGATC 1 cut(s) 115
Ama87I CYCGRG 1 cut(s) 319
AoxI GGCC 2 cut(s) 220, 322
ApeKI GCWGC 3 cut(s) 286, 540, 629
ArsI GACNNNNNNTTYG 2 cut(s) 338, 370
AsiGI ACCGGT 1 cut(s) 174
Asp700I GAANNNNTTC 2 cut(s) 684, 730
Asp718I GGTACC 1 cut(s) 302
AspS9I GGNCC 2 cut(s) 322, 392
AsuC2I CCSGG 4 cut(s) 78, 320, 321, 762
AsuHPI GGTGA 1 cut(s) 768
AvaI CYCGRG 1 cut(s) 319
AvaII GGWCC 1 cut(s) 392
BaeGI GKGCMC 1 cut(s) 83
BaeI ACNNNNGTAYC 4 cut(s) 286, 294, 319, 327
BanI GGYRCC 2 cut(s) 80, 302
BbsI GAAGAC 1 cut(s) 322
Bbv12I GWGCWC 1 cut(s) 571
BbvCI CCTCAGC 1 cut(s) 438
BbvI GCAGC 3 cut(s) 273, 552, 616
BccI CCATC 5 cut(s) 92, 98, 269, 557, 658
BceAI ACGGC 3 cut(s) 117, 271, 527
BciT130I CCWGG 1 cut(s) 723
BcnI CCSGG 4 cut(s) 78, 320, 321, 762
BfaI CTAG 2 cut(s) 404, 668
BisI GCNGC 4 cut(s) 102, 287, 541, 630
BlsI GCNGC 4 cut(s) 103, 288, 542, 631
Bme1390I CCNGG 5 cut(s) 78, 320, 321, 723, 762
Bme18I GGWCC 1 cut(s) 392
BmeT110I CYCGRG 1 cut(s) 319
BmgBI CACGTC 2 cut(s) 310, 328
BmgT120I GGNCC 2 cut(s) 322, 392
BmiI GGNNCC 4 cut(s) 82, 236, 259, 304
BmrFI CCNGG 5 cut(s) 78, 320, 321, 723, 762
BmsI GCATC 2 cut(s) 304, 776
BpiI GAAGAC 1 cut(s) 322
Bpu10I CCTNAGC 1 cut(s) 438
BpuMI CCSGG 4 cut(s) 78, 320, 321, 762
BsaHI GRCGYC 2 cut(s) 348, 714
BsaJI CCNNGG 4 cut(s) 318, 319, 416, 465
BsaWI WCCGGW 1 cut(s) 174
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 1 cut(s) 376
Bse118I RCCGGY 2 cut(s) 174, 350
Bse1I ACTGG 3 cut(s) 223, 304, 711
Bse3DI GCAATG 1 cut(s) 398
BseBI CCWGG 1 cut(s) 723
BseDI CCNNGG 4 cut(s) 318, 319, 416, 465
BseGI GGATG 4 cut(s) 385, 568, 650, 715
BseLI CCNNNNNNNGG 1 cut(s) 376
BseMI GCAATG 1 cut(s) 398
BseMII CTCAG 2 cut(s) 267, 429
BseNI ACTGG 3 cut(s) 223, 304, 711
BseRI GAGGAG 4 cut(s) 8, 11, 22, 117
BseSI GKGCMC 1 cut(s) 83
BseXI GCAGC 3 cut(s) 273, 552, 616
BsgI GTGCAG 1 cut(s) 786
BshFI GGCC 2 cut(s) 222, 324
BshNI GGYRCC 2 cut(s) 80, 302
BshTI ACCGGT 1 cut(s) 174
BsiHKAI GWGCWC 1 cut(s) 571
BsiHKCI CYCGRG 1 cut(s) 319
BsiSI CCGG 5 cut(s) 77, 175, 320, 351, 761
BslI CCNNNNNNNGG 1 cut(s) 376
BsmI GAATGC 1 cut(s) 789
BsnI GGCC 2 cut(s) 222, 324
BsoBI CYCGRG 1 cut(s) 319
Bsp1286I GDGCHC 2 cut(s) 83, 571
Bsp143I GATC 2 cut(s) 120, 334
BspACI CCGC 2 cut(s) 102, 435
BspANI GGCC 2 cut(s) 222, 324
BspCNI CTCAG 2 cut(s) 268, 430
BspLI GGNNCC 4 cut(s) 82, 236, 259, 304
BspPI GGATC 1 cut(s) 115
BspT107I GGYRCC 2 cut(s) 80, 302
BsrDI GCAATG 1 cut(s) 398
BsrFI RCCGGY 2 cut(s) 174, 350
BsrI ACTGG 3 cut(s) 223, 304, 711
BssAI RCCGGY 2 cut(s) 174, 350
BssECI CCNNGG 4 cut(s) 318, 319, 416, 465
BssMI GATC 2 cut(s) 120, 334
BssNI GRCGYC 2 cut(s) 348, 714
BssT1I CCWWGG 1 cut(s) 465
Bst2UI CCWGG 1 cut(s) 723
Bst4CI ACNGT 3 cut(s) 146, 215, 573
BstACI GRCGYC 2 cut(s) 348, 714
BstC8I GCNNGC 2 cut(s) 195, 352
BstDEI CTNAG 2 cut(s) 276, 438
BstEII GGTNACC 1 cut(s) 774
BstF5I GGATG 4 cut(s) 385, 568, 650, 715
BstKTI GATC 2 cut(s) 123, 337
BstMBI GATC 2 cut(s) 120, 334
BstMWI GCNNNNNNNGC 1 cut(s) 59
BstNI CCWGG 1 cut(s) 723
BstPI GGTNACC 1 cut(s) 774
BstSCI CCNGG 5 cut(s) 76, 318, 319, 721, 760
BstSLI GKGCMC 1 cut(s) 83
BstV1I GCAGC 3 cut(s) 273, 552, 616
BstV2I GAAGAC 1 cut(s) 322
BsuRI GGCC 2 cut(s) 222, 324
BtgZI GCGATG 1 cut(s) 247
BtrI CACGTC 2 cut(s) 310, 328
BtsCI GGATG 4 cut(s) 385, 568, 650, 715
BtsI GCAGTG 1 cut(s) 739
BtsIMutI CAGTG 4 cut(s) 297, 578, 588, 739
Cac8I GCNNGC 2 cut(s) 195, 352
Cfr10I RCCGGY 2 cut(s) 174, 350
Cfr13I GGNCC 2 cut(s) 322, 392
Cfr9I CCCGGG 1 cut(s) 319
CseI GACGC 1 cut(s) 356
Csp6I GTAC 3 cut(s) 178, 303, 473
CspAI ACCGGT 1 cut(s) 174
CviAII CATG 2 cut(s) 190, 247
CviQI GTAC 3 cut(s) 178, 303, 473
DdeI CTNAG 2 cut(s) 276, 438
DpnI GATC 2 cut(s) 122, 336
DpnII GATC 2 cut(s) 120, 334
EaeI YGGCCR 1 cut(s) 220
Eco130I CCWWGG 1 cut(s) 465
Eco47I GGWCC 1 cut(s) 392
Eco88I CYCGRG 1 cut(s) 319
Eco91I GGTNACC 1 cut(s) 774
EcoO65I GGTNACC 1 cut(s) 774
EcoRII CCWGG 1 cut(s) 721
EcoT14I CCWWGG 1 cut(s) 465
EcoT22I ATGCAT 1 cut(s) 791
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 2 cut(s) 193, 250
FatI CATG 2 cut(s) 189, 246
FauNDI CATATG 1 cut(s) 634
Fnu4HI GCNGC 4 cut(s) 102, 287, 541, 630
FokI GGATG 4 cut(s) 392, 575, 637, 722
Fsp4HI GCNGC 4 cut(s) 102, 287, 541, 630
FspBI CTAG 2 cut(s) 404, 668
GluI GCNGC 4 cut(s) 102, 287, 541, 630
HaeIII GGCC 2 cut(s) 222, 324
HapII CCGG 5 cut(s) 77, 175, 320, 351, 761
HgaI GACGC 1 cut(s) 356
Hin1I GRCGYC 2 cut(s) 348, 714
Hin1II CATG 2 cut(s) 193, 250
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HinfI GANTC 2 cut(s) 156, 646
HpaII CCGG 5 cut(s) 77, 175, 320, 351, 761
HphI GGTGA 1 cut(s) 768
Hpy166II GTNNAC 3 cut(s) 142, 409, 739
Hpy188I TCNGA 4 cut(s) 277, 417, 491, 608
Hpy188III TCNNGA 1 cut(s) 668
Hpy8I GTNNAC 3 cut(s) 142, 409, 739
Hpy99I CGWCG 6 cut(s) 53, 215, 314, 350, 480, 719
HpyAV CCTTC 3 cut(s) 78, 665, 764
HpyCH4III ACNGT 3 cut(s) 146, 215, 573
HpyCH4IV ACGT 5 cut(s) 309, 327, 411, 475, 714
HpyCH4V TGCA 4 cut(s) 398, 632, 767, 789
HpyF10VI GCNNNNNNNGC 1 cut(s) 59
HpyF3I CTNAG 2 cut(s) 276, 438
HpySE526I ACGT 5 cut(s) 309, 327, 411, 475, 714
Hsp92I GRCGYC 2 cut(s) 348, 714
Hsp92II CATG 2 cut(s) 193, 250
KpnI GGTACC 1 cut(s) 306
KroI GCCGGC 1 cut(s) 350
KroNI GCCGGC 1 cut(s) 352
Kzo9I GATC 2 cut(s) 120, 334
LmnI GCTCC 3 cut(s) 240, 263, 566
Lsp1109I GCAGC 3 cut(s) 273, 552, 616
LweI GCATC 2 cut(s) 304, 776
MaeI CTAG 2 cut(s) 404, 668
MaeII ACGT 5 cut(s) 309, 327, 411, 475, 714
MaeIII GTNAC 1 cut(s) 774
MalI GATC 2 cut(s) 122, 336
MboI GATC 2 cut(s) 120, 334
MboII GAAGA 4 cut(s) 151, 262, 322, 436
MhlI GDGCHC 2 cut(s) 83, 571
MluCI AATT 1 cut(s) 609
MmeI TCCRAC 1 cut(s) 586
Mph1103I ATGCAT 1 cut(s) 791
MroNI GCCGGC 1 cut(s) 350
MroXI GAANNNNTTC 2 cut(s) 684, 730
MseI TTAA 4 cut(s) 387, 497, 597, 701
MslI CAYNNNNRTG 2 cut(s) 188, 312
MspA1I CMGCKG 1 cut(s) 437
MspI CCGG 5 cut(s) 77, 175, 320, 351, 761
MspR9I CCNGG 5 cut(s) 78, 320, 321, 723, 762
Mva1269I GAATGC 1 cut(s) 789
MvaI CCWGG 1 cut(s) 723
MwoI GCNNNNNNNGC 1 cut(s) 59
NaeI GCCGGC 1 cut(s) 352
NciI CCSGG 4 cut(s) 78, 320, 321, 762
NdeI CATATG 1 cut(s) 634
NdeII GATC 2 cut(s) 120, 334
NgoMIV GCCGGC 1 cut(s) 350
NlaIII CATG 2 cut(s) 193, 250
NlaIV GGNNCC 4 cut(s) 82, 236, 259, 304
NmuCI GTSAC 1 cut(s) 774
NsiI ATGCAT 1 cut(s) 791
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PctI GAATGC 1 cut(s) 789
PdiI GCCGGC 1 cut(s) 352
PdmI GAANNNNTTC 2 cut(s) 684, 730
PfeI GAWTC 2 cut(s) 156, 646
PflFI GACNNNGTC 1 cut(s) 215
PinAI ACCGGT 1 cut(s) 174
PkrI GCNGC 4 cut(s) 103, 288, 542, 631
Psp6I CCWGG 1 cut(s) 721
PspEI GGTNACC 1 cut(s) 774
PspGI CCWGG 1 cut(s) 721
PspN4I GGNNCC 4 cut(s) 82, 236, 259, 304
PspPI GGNCC 2 cut(s) 322, 392
PsyI GACNNNGTC 1 cut(s) 215
RsaI GTAC 3 cut(s) 179, 304, 474
RsaNI GTAC 3 cut(s) 178, 303, 473
RseI CAYNNNNRTG 2 cut(s) 188, 312
SaqAI TTAA 4 cut(s) 387, 497, 597, 701
SatI GCNGC 4 cut(s) 102, 287, 541, 630
Sau3AI GATC 2 cut(s) 120, 334
Sau96I GGNCC 2 cut(s) 322, 392
ScrFI CCNGG 5 cut(s) 78, 320, 321, 723, 762
SduI GDGCHC 2 cut(s) 83, 571
SfaNI GCATC 2 cut(s) 304, 776
SinI GGWCC 1 cut(s) 392
SmaI CCCGGG 1 cut(s) 321
SmiMI CAYNNNNRTG 2 cut(s) 188, 312
Sse9I AATT 1 cut(s) 609
SsiI CCGC 2 cut(s) 102, 435
SspMI CTAG 2 cut(s) 404, 668
StyD4I CCNGG 5 cut(s) 76, 318, 319, 721, 760
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 3 cut(s) 146, 215, 573
TaiI ACGT 5 cut(s) 312, 330, 414, 478, 717
TaqI TCGA 8 cut(s) 93, 162, 312, 333, 345, 357, 363, 717
TasI AATT 1 cut(s) 609
TauI GCSGC 1 cut(s) 104
TfiI GAWTC 2 cut(s) 156, 646
Tru1I TTAA 4 cut(s) 387, 497, 597, 701
Tru9I TTAA 4 cut(s) 387, 497, 597, 701
TscAI CASTG 4 cut(s) 304, 578, 595, 746
TseFI GTSAC 1 cut(s) 774
TseI GCWGC 3 cut(s) 286, 540, 629
Tsp45I GTSAC 1 cut(s) 774
TspDTI ATGAA 4 cut(s) 17, 263, 296, 461
TspGWI ACGGA 2 cut(s) 37, 613
TspMI CCCGGG 1 cut(s) 319
TspRI CASTG 4 cut(s) 304, 578, 595, 746
Tth111I GACNNNGTC 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 392
XbaI TCTAGA 1 cut(s) 667
XmaI CCCGGG 1 cut(s) 319
XmnI GAANNNNTTC 2 cut(s) 684, 730
XspI CTAG 2 cut(s) 404, 668
ZraI GACGTC 1 cut(s) 715
Zsp2I ATGCAT 1 cut(s) 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.