Rh6BG071600

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000645
Physical Location & Seq
Reverse (-)
10823999 .. 10825721
1723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG071600.1

Sequence Viewer

Length: 804 bp
ATGAATAACACCCCCCTACTCCATCACCTCGTCCTTCTTCTTCTGCTACCATGCACCCTAGTATACACCATCACCACCGTCACTTCATCCTCCGCCAACCAGGCTTACCCCACCCTCACCGAGGACTGCTCAGTGTCCGGTGAGGATATCCCTGTGCCCATACGGAGGGAGGTGTATGATAACGGTCGAATATTTGACATCAGCCACAGGTACACCCCTGACATGCCCAGTTATGGAGTTAAGGACGGGCTTGGGGAATTCCTGTCGCTTGGCATGAGCATCAAAAATGGCTCGGACGCCAACGTTTCAGTGATGAAGTTCAGTGTCCACTCCGCCACCCATGTGGACGCGCCGGGTCATGTCTTCGACCATTACTACGATGCTGGCTTTGATGTTGATGCGCTTGACCTCGAATTCTTAAATGGTCCTGCACTGGTGGTTGATGTTCCGAGGAATAAGAACATAACTGCTGAAGTCATGGAGGCTTTAAATATTCCTAAAGGGGTGCGTCGTGTGCTTTTCAGAACCCTAAATACCGACAGGAAGCTTATGTGGAAGAAAGAGTTTGATACAAGCGCTGTAGGGTTTATGGAGGATGGGGCAAAATGGCTGGTGGACAACACAGACATTAAACTTATTGGGCTTGATTACTTAAACGTCGCAGCTTCTGTTGATGTATTGCCAGTTCATTATGCGTTTCTACAAGGCCGGGAGATGGTTCTTGTTGAGGGTTTAAAGCTCGATGGAGTTCCAGCAGGAATATATTCTGTCCATTGTTTGCCTTTAAGGGTTGATCGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

267

Amino Acids

29.6

Weight (kDa)

5.19

Isoelectric Point (pI)

33.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 65 - 220 1.2e-19 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 63
AccII CGCG 1 cut(s) 350
AciI CCGC 2 cut(s) 93, 333
AclI AACGTT 1 cut(s) 303
AcsI RAATTY 2 cut(s) 257, 413
AcuI CTGAAG 1 cut(s) 492
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 1 cut(s) 212
AfeI AGCGCT 1 cut(s) 577
AfiI CCNNNNNNNGG 4 cut(s) 121, 165, 233, 715
AjnI CCWGG 1 cut(s) 99
AleI CACNNNNGTG 1 cut(s) 341
AluBI AGCT 3 cut(s) 547, 665, 739
AluI AGCT 3 cut(s) 547, 665, 739
AlwNI CAGNNNCTG 1 cut(s) 668
Aor51HI AGCGCT 1 cut(s) 577
AoxI GGCC 1 cut(s) 706
ApeKI GCWGC 1 cut(s) 662
ApoI RAATTY 2 cut(s) 257, 413
Asp700I GAANNNNTTC 1 cut(s) 763
AspLEI GCGC 3 cut(s) 352, 403, 578
AspS9I GGNCC 1 cut(s) 425
AsuC2I CCSGG 2 cut(s) 354, 710
AsuHPI GGTGA 4 cut(s) 17, 64, 109, 152
AvaII GGWCC 1 cut(s) 425
BaeGI GKGCMC 1 cut(s) 159
BbsI GAAGAC 1 cut(s) 355
BbvI GCAGC 1 cut(s) 674
BccI CCATC 5 cut(s) 30, 77, 590, 709, 737
BciT130I CCWGG 1 cut(s) 101
BcnI CCSGG 2 cut(s) 354, 710
BfaI CTAG 1 cut(s) 59
BfmI CTRYAG 1 cut(s) 579
BfoI RGCGCY 1 cut(s) 579
BglI GCCNNNNNGGC 1 cut(s) 101
BisI GCNGC 1 cut(s) 663
BlsI GCNGC 1 cut(s) 664
Bme1390I CCNGG 3 cut(s) 101, 354, 710
Bme18I GGWCC 1 cut(s) 425
BmgT120I GGNCC 1 cut(s) 425
BmrFI CCNGG 3 cut(s) 101, 354, 710
BmrI ACTGGG 1 cut(s) 222
BmsI GCATC 3 cut(s) 288, 370, 388
BmuI ACTGGG 1 cut(s) 222
BpiI GAAGAC 1 cut(s) 355
BplI GAGNNNNNCTC 2 cut(s) 113, 145
BpuMI CCSGG 2 cut(s) 354, 710
BsaHI GRCGYC 1 cut(s) 297
BsaJI CCNNGG 2 cut(s) 120, 449
BsaWI WCCGGW 1 cut(s) 137
Bsc4I CCNNNNNNNGG 4 cut(s) 121, 165, 233, 715
Bse1I ACTGG 3 cut(s) 228, 438, 683
BseBI CCWGG 1 cut(s) 101
BseDI CCNNGG 2 cut(s) 120, 449
BseGI GGATG 2 cut(s) 86, 601
BseLI CCNNNNNNNGG 4 cut(s) 121, 165, 233, 715
BseMII CTCAG 1 cut(s) 144
BseNI ACTGG 3 cut(s) 228, 438, 683
BseSI GKGCMC 1 cut(s) 159
BseXI GCAGC 1 cut(s) 674
BsgI GTGCAG 1 cut(s) 414
Bsh1236I CGCG 1 cut(s) 350
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 1 cut(s) 708
BsiEI CGRYCG 1 cut(s) 187
BsiSI CCGG 3 cut(s) 138, 353, 709
BslI CCNNNNNNNGG 4 cut(s) 121, 165, 233, 715
BsnI GGCC 1 cut(s) 708
Bsp1286I GDGCHC 1 cut(s) 159
Bsp143I GATC 1 cut(s) 793
BspACI CCGC 2 cut(s) 93, 333
BspANI GGCC 1 cut(s) 708
BspCNI CTCAG 1 cut(s) 143
BspFNI CGCG 1 cut(s) 350
BsrI ACTGG 3 cut(s) 228, 438, 683
BssECI CCNNGG 2 cut(s) 120, 449
BssMI GATC 1 cut(s) 793
BssNAI GTATAC 1 cut(s) 64
BssNI GRCGYC 1 cut(s) 297
Bst1107I GTATAC 1 cut(s) 64
Bst2UI CCWGG 1 cut(s) 101
Bst4CI ACNGT 2 cut(s) 79, 185
BstACI GRCGYC 1 cut(s) 297
BstC8I GCNNGC 1 cut(s) 385
BstDEI CTNAG 1 cut(s) 130
BstENI CCTNNNNNAGG 1 cut(s) 119
BstF5I GGATG 2 cut(s) 86, 601
BstFNI CGCG 1 cut(s) 350
BstH2I RGCGCY 1 cut(s) 579
BstHHI GCGC 3 cut(s) 352, 403, 578
BstKTI GATC 1 cut(s) 796
BstMBI GATC 1 cut(s) 793
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 2 cut(s) 101, 514
BstNI CCWGG 1 cut(s) 101
BstNSI RCATGY 1 cut(s) 226
BstSCI CCNGG 3 cut(s) 99, 352, 708
BstSFI CTRYAG 1 cut(s) 579
BstSLI GKGCMC 1 cut(s) 159
BstUI CGCG 1 cut(s) 350
BstV1I GCAGC 1 cut(s) 674
BstV2I GAAGAC 1 cut(s) 355
BstXI CCANNNNNNTGG 1 cut(s) 343
BstZ17I GTATAC 1 cut(s) 64
BsuRI GGCC 1 cut(s) 708
BtsCI GGATG 2 cut(s) 86, 601
BtsIMutI CAGTG 4 cut(s) 138, 315, 328, 431
Cac8I GCNNGC 1 cut(s) 385
CaiI CAGNNNCTG 1 cut(s) 668
CfoI GCGC 3 cut(s) 352, 403, 578
Cfr13I GGNCC 1 cut(s) 425
CseI GACGC 3 cut(s) 305, 356, 497
Csp6I GTAC 1 cut(s) 211
CviAII CATG 6 cut(s) 51, 223, 274, 341, 359, 478
CviQI GTAC 1 cut(s) 211
DdeI CTNAG 1 cut(s) 130
DpnI GATC 1 cut(s) 795
DpnII GATC 1 cut(s) 793
DraI TTTAAA 2 cut(s) 489, 735
EciI GGCGGA 2 cut(s) 82, 322
Eco32I GATATC 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 425
Eco47III AGCGCT 1 cut(s) 577
Eco57I CTGAAG 1 cut(s) 492
EcoNI CCTNNNNNAGG 1 cut(s) 119
EcoRI GAATTC 2 cut(s) 257, 413
EcoRII CCWGG 1 cut(s) 99
EcoRV GATATC 1 cut(s) 148
FaeI CATG 6 cut(s) 54, 226, 277, 344, 362, 481
FatI CATG 6 cut(s) 50, 222, 273, 340, 358, 477
FblI GTMKAC 1 cut(s) 63
Fnu4HI GCNGC 1 cut(s) 663
FokI GGATG 2 cut(s) 73, 608
Fsp4HI GCNGC 1 cut(s) 663
FspBI CTAG 1 cut(s) 59
GlaI GCGC 3 cut(s) 351, 402, 577
GluI GCNGC 1 cut(s) 663
HaeII RGCGCY 1 cut(s) 579
HaeIII GGCC 1 cut(s) 708
HapII CCGG 3 cut(s) 138, 353, 709
HgaI GACGC 3 cut(s) 305, 356, 497
HhaI GCGC 3 cut(s) 352, 403, 578
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 6 cut(s) 54, 226, 277, 344, 362, 481
Hin6I GCGC 3 cut(s) 350, 401, 576
HinP1I GCGC 3 cut(s) 350, 401, 576
HindIII AAGCTT 1 cut(s) 545
HpaII CCGG 3 cut(s) 138, 353, 709
HphI GGTGA 4 cut(s) 17, 64, 109, 152
Hpy166II GTNNAC 5 cut(s) 64, 213, 328, 346, 616
Hpy188I TCNGA 4 cut(s) 295, 450, 524, 803
Hpy8I GTNNAC 5 cut(s) 64, 213, 328, 346, 616
Hpy99I CGWCG 2 cut(s) 513, 662
HpyAV CCTTC 1 cut(s) 44
HpyCH4III ACNGT 2 cut(s) 79, 185
HpyCH4IV ACGT 2 cut(s) 303, 657
HpyCH4V TGCA 2 cut(s) 54, 431
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 514
HpyF3I CTNAG 1 cut(s) 130
HpySE526I ACGT 2 cut(s) 303, 657
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 6 cut(s) 54, 226, 277, 344, 362, 481
HspAI GCGC 3 cut(s) 350, 401, 576
Kzo9I GATC 1 cut(s) 793
Lsp1109I GCAGC 1 cut(s) 674
LweI GCATC 3 cut(s) 288, 370, 388
MaeI CTAG 1 cut(s) 59
MaeII ACGT 2 cut(s) 303, 657
MaeIII GTNAC 1 cut(s) 79
MalI GATC 1 cut(s) 795
MboI GATC 1 cut(s) 793
MboII GAAGA 4 cut(s) 29, 32, 355, 568
MhlI GDGCHC 1 cut(s) 159
MluCI AATT 2 cut(s) 257, 413
MroXI GAANNNNTTC 1 cut(s) 763
MseI TTAA 7 cut(s) 240, 419, 488, 630, 653, 734, 785
MslI CAYNNNNRTG 1 cut(s) 341
MspI CCGG 3 cut(s) 138, 353, 709
MspR9I CCNGG 3 cut(s) 101, 354, 710
MvaI CCWGG 1 cut(s) 101
MvnI CGCG 1 cut(s) 350
MwoI GCNNNNNNNGC 2 cut(s) 101, 514
NciI CCSGG 2 cut(s) 354, 710
NdeII GATC 1 cut(s) 793
NlaIII CATG 6 cut(s) 54, 226, 277, 344, 362, 481
NmuCI GTSAC 1 cut(s) 79
NspI RCATGY 1 cut(s) 226
OliI CACNNNNGTG 1 cut(s) 341
PdmI GAANNNNTTC 1 cut(s) 763
PkrI GCNGC 1 cut(s) 664
Psp1406I AACGTT 1 cut(s) 303
Psp6I CCWGG 1 cut(s) 99
PspGI CCWGG 1 cut(s) 99
PspPI GGNCC 1 cut(s) 425
PsrI GAACNNNNNNTAC 4 cut(s) 517, 549, 669, 701
PstNI CAGNNNCTG 1 cut(s) 668
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
RseI CAYNNNNRTG 1 cut(s) 341
SaqAI TTAA 7 cut(s) 240, 419, 488, 630, 653, 734, 785
SatI GCNGC 1 cut(s) 663
Sau3AI GATC 1 cut(s) 793
Sau96I GGNCC 1 cut(s) 425
ScrFI CCNGG 3 cut(s) 101, 354, 710
SduI GDGCHC 1 cut(s) 159
SetI ASST 9 cut(s) 30, 174, 212, 306, 411, 549, 660, 667, 741
SfaNI GCATC 3 cut(s) 288, 370, 388
SfcI CTRYAG 1 cut(s) 579
SinI GGWCC 1 cut(s) 425
SmiMI CAYNNNNRTG 1 cut(s) 341
Sse9I AATT 2 cut(s) 257, 413
SsiI CCGC 2 cut(s) 93, 333
SspI AATATT 2 cut(s) 192, 493
SspMI CTAG 1 cut(s) 59
StyD4I CCNGG 3 cut(s) 99, 352, 708
TaaI ACNGT 2 cut(s) 79, 185
TaiI ACGT 2 cut(s) 306, 660
TaqI TCGA 4 cut(s) 187, 366, 411, 741
TasI AATT 2 cut(s) 257, 413
Tru1I TTAA 7 cut(s) 240, 419, 488, 630, 653, 734, 785
Tru9I TTAA 7 cut(s) 240, 419, 488, 630, 653, 734, 785
TscAI CASTG 4 cut(s) 138, 315, 328, 438
TseFI GTSAC 1 cut(s) 79
TseI GCWGC 1 cut(s) 662
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 4 cut(s) 17, 75, 329, 677
TspGWI ACGGA 1 cut(s) 178
TspRI CASTG 4 cut(s) 138, 315, 328, 438
VpaK11BI GGWCC 1 cut(s) 425
XagI CCTNNNNNAGG 1 cut(s) 119
XapI RAATTY 2 cut(s) 257, 413
XceI RCATGY 1 cut(s) 226
XmiI GTMKAC 1 cut(s) 63
XmnI GAANNNNTTC 1 cut(s) 763
XspI CTAG 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.