Rw2G024460

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000645
Physical Location & Seq
Reverse (-)
36100072 .. 36104506
4435 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G024460.1

Sequence Viewer

Length: 468 bp
CTAGGCCAGTTCCTCTGGCTCCCCAACAGCATGAAAAACAGCTCCATTGCTAACAACTCCGAGATGAAGCTCCCCACCCACACAAGGACCCACGTCGACGCCCCCGACCACGTCTTCGATCACTGCTTCGACGTCGGCTTCGATGTCGATACTCTTGACTTGGATGTCCAGGGTGCCCAGAGCAATTTATGGGTGCCCAGAGCAATTTATGGTTCTGTAGCCGGATTAGGGATTCCAGTGAACGGTCGCCGGATTCTGGTCACCGGTCGCCGAGTCAGTCACCGGCCTGCATTATTATTCAATAAAAATGAAAATATCAGCCGCATGCAGATACTAATAGAACATTACTCTGACGATCATTTTGCTTGTCATTTGCACATAAGAGAGTTGAGATTTGAGAATGATGATCAAATGCAATACCTAGCGTTTCAGGGATCTATATATAGAGTTTCATGTTTGGTGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

155

Amino Acids

17.76

Weight (kDa)

6.52

Isoelectric Point (pI)

49.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
AatII GACGTC 1 cut(s) 135
AccB1I GGYRCC 2 cut(s) 173, 193
AccI GTMKAC 1 cut(s) 96
AciI CCGC 1 cut(s) 322
AclWI GGATC 1 cut(s) 442
AcyI GRCGYC 2 cut(s) 99, 132
AfiI CCNNNNNNNGG 4 cut(s) 84, 228, 242, 256
AgeI ACCGGT 1 cut(s) 263
AgsI TTSAA 1 cut(s) 301
AjiI CACGTC 2 cut(s) 94, 112
AjnI CCWGG 1 cut(s) 168
AluBI AGCT 2 cut(s) 42, 70
AluI AGCT 2 cut(s) 42, 70
AlwI GGATC 1 cut(s) 442
AoxI GGCC 2 cut(s) 4, 284
ArsI GACNNNNNNTTYG 6 cut(s) 98, 122, 130, 154, 344, 376
AsiGI ACCGGT 1 cut(s) 263
AspS9I GGNCC 1 cut(s) 87
AsuHPI GGTGA 2 cut(s) 253, 272
AvaII GGWCC 1 cut(s) 87
BaeGI GKGCMC 2 cut(s) 178, 198
BanI GGYRCC 2 cut(s) 173, 193
BbsI GAAGAC 1 cut(s) 106
BcgI CGANNNNNNTGC 2 cut(s) 344, 378
BciT130I CCWGG 1 cut(s) 170
BclI TGATCA 1 cut(s) 406
BfaI CTAG 2 cut(s) 2, 422
BfmI CTRYAG 1 cut(s) 216
BisI GCNGC 1 cut(s) 322
BlsI GCNGC 1 cut(s) 323
Bme1390I CCNGG 1 cut(s) 170
Bme18I GGWCC 1 cut(s) 87
BmgBI CACGTC 2 cut(s) 94, 112
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 4 cut(s) 20, 89, 175, 195
BmrFI CCNGG 1 cut(s) 170
BoxI GACNNNNGTC 1 cut(s) 92
BpiI GAAGAC 1 cut(s) 106
BsaHI GRCGYC 2 cut(s) 99, 132
BsaJI CCNNGG 1 cut(s) 169
BsaWI WCCGGW 1 cut(s) 263
Bsc4I CCNNNNNNNGG 4 cut(s) 84, 228, 242, 256
Bse118I RCCGGY 2 cut(s) 263, 282
Bse1I ACTGG 2 cut(s) 7, 236
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 1 cut(s) 169
BseGI GGATG 1 cut(s) 169
BseLI CCNNNNNNNGG 4 cut(s) 84, 228, 242, 256
BseMI GCAATG 1 cut(s) 45
BseNI ACTGG 2 cut(s) 7, 236
BseSI GKGCMC 2 cut(s) 178, 198
Bsh1285I CGRYCG 2 cut(s) 247, 268
BshFI GGCC 2 cut(s) 6, 286
BshNI GGYRCC 2 cut(s) 173, 193
BshTI ACCGGT 1 cut(s) 263
BsiEI CGRYCG 2 cut(s) 247, 268
BsiSI CCGG 4 cut(s) 222, 250, 264, 283
BslI CCNNNNNNNGG 4 cut(s) 84, 228, 242, 256
BsnI GGCC 2 cut(s) 6, 286
Bsp1286I GDGCHC 2 cut(s) 178, 198
Bsp143I GATC 4 cut(s) 118, 355, 406, 434
BspACI CCGC 1 cut(s) 322
BspANI GGCC 2 cut(s) 6, 286
BspLI GGNNCC 4 cut(s) 20, 89, 175, 195
BspPI GGATC 1 cut(s) 442
BspT107I GGYRCC 2 cut(s) 173, 193
BsrDI GCAATG 1 cut(s) 45
BsrFI RCCGGY 2 cut(s) 263, 282
BsrI ACTGG 2 cut(s) 7, 236
BssAI RCCGGY 2 cut(s) 263, 282
BssECI CCNNGG 1 cut(s) 169
BssMI GATC 4 cut(s) 118, 355, 406, 434
BssNI GRCGYC 2 cut(s) 99, 132
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 1 cut(s) 245
BstACI GRCGYC 2 cut(s) 99, 132
BstC8I GCNNGC 2 cut(s) 288, 326
BstEII GGTNACC 1 cut(s) 259
BstF5I GGATG 1 cut(s) 169
BstKTI GATC 4 cut(s) 121, 358, 409, 437
BstMBI GATC 4 cut(s) 118, 355, 406, 434
BstMCI CGRYCG 2 cut(s) 247, 268
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 328
BstPAI GACNNNNGTC 1 cut(s) 92
BstPI GGTNACC 1 cut(s) 259
BstSCI CCNGG 1 cut(s) 168
BstSFI CTRYAG 1 cut(s) 216
BstSLI GKGCMC 2 cut(s) 178, 198
BstV2I GAAGAC 1 cut(s) 106
BstX2I RGATCY 1 cut(s) 434
BstYI RGATCY 1 cut(s) 434
BsuRI GGCC 2 cut(s) 6, 286
BtrI CACGTC 2 cut(s) 94, 112
BtsCI GGATG 1 cut(s) 169
BtsI GCAGTG 1 cut(s) 121
BtsIMutI CAGTG 2 cut(s) 121, 243
Cac8I GCNNGC 2 cut(s) 288, 326
Cfr10I RCCGGY 2 cut(s) 263, 282
Cfr13I GGNCC 1 cut(s) 87
CseI GACGC 1 cut(s) 107
CspAI ACCGGT 1 cut(s) 263
CviAII CATG 3 cut(s) 31, 325, 453
CviJI RGCY 8 cut(s) 6, 19, 42, 70, 138, 221, 286, 321
CviKI_1 RGCY 8 cut(s) 6, 19, 42, 70, 138, 221, 286, 321
DpnI GATC 4 cut(s) 120, 357, 408, 436
DpnII GATC 4 cut(s) 118, 355, 406, 434
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
Eco47I GGWCC 1 cut(s) 87
Eco91I GGTNACC 1 cut(s) 259
EcoO109I RGGNCCY 1 cut(s) 87
EcoO65I GGTNACC 1 cut(s) 259
EcoRII CCWGG 1 cut(s) 168
FaeI CATG 3 cut(s) 34, 328, 456
FaiI YATR 9 cut(s) 32, 190, 210, 326, 380, 440, 442, 444, 454
FatI CATG 3 cut(s) 30, 324, 452
FbaI TGATCA 1 cut(s) 406
FblI GTMKAC 1 cut(s) 96
Fnu4HI GCNGC 1 cut(s) 322
FokI GGATG 1 cut(s) 176
Fsp4HI GCNGC 1 cut(s) 322
FspBI CTAG 2 cut(s) 2, 422
GluI GCNGC 1 cut(s) 322
HaeIII GGCC 2 cut(s) 6, 286
HapII CCGG 4 cut(s) 222, 250, 264, 283
HgaI GACGC 1 cut(s) 107
Hin1I GRCGYC 2 cut(s) 99, 132
Hin1II CATG 3 cut(s) 34, 328, 456
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HinfI GANTC 3 cut(s) 232, 253, 273
HpaII CCGG 4 cut(s) 222, 250, 264, 283
HphI GGTGA 2 cut(s) 253, 272
Hpy166II GTNNAC 2 cut(s) 97, 241
Hpy188I TCNGA 2 cut(s) 61, 352
Hpy188III TCNNGA 1 cut(s) 155
Hpy8I GTNNAC 2 cut(s) 97, 241
Hpy99I CGWCG 4 cut(s) 98, 101, 134, 137
HpyCH4III ACNGT 1 cut(s) 245
HpyCH4IV ACGT 3 cut(s) 93, 111, 132
HpyCH4V TGCA 4 cut(s) 290, 328, 376, 415
HpySE526I ACGT 3 cut(s) 93, 111, 132
Hsp92I GRCGYC 2 cut(s) 99, 132
Hsp92II CATG 3 cut(s) 34, 328, 456
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 4 cut(s) 118, 355, 406, 434
LmnI GCTCC 3 cut(s) 24, 47, 75
MaeI CTAG 2 cut(s) 2, 422
MaeII ACGT 3 cut(s) 93, 111, 132
MaeIII GTNAC 2 cut(s) 259, 278
MalI GATC 4 cut(s) 120, 357, 408, 436
MboI GATC 4 cut(s) 118, 355, 406, 434
MboII GAAGA 1 cut(s) 106
MflI RGATCY 1 cut(s) 434
MhlI GDGCHC 2 cut(s) 178, 198
MluCI AATT 2 cut(s) 184, 204
MlyI GAGTC 1 cut(s) 282
MnlI CCTC 1 cut(s) 23
MspI CCGG 4 cut(s) 222, 250, 264, 283
MspR9I CCNGG 1 cut(s) 170
MvaI CCWGG 1 cut(s) 170
NdeII GATC 4 cut(s) 118, 355, 406, 434
NlaIII CATG 3 cut(s) 34, 328, 456
NlaIV GGNNCC 4 cut(s) 20, 89, 175, 195
NmeAIII GCCGAG 1 cut(s) 296
NmuCI GTSAC 2 cut(s) 259, 278
NspI RCATGY 1 cut(s) 328
PaeI GCATGC 1 cut(s) 328
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 138
PfeI GAWTC 2 cut(s) 232, 253
PflFI GACNNNGTC 1 cut(s) 110
PinAI ACCGGT 1 cut(s) 263
PkrI GCNGC 1 cut(s) 323
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
PpuMI RGGWCCY 1 cut(s) 87
PshAI GACNNNNGTC 1 cut(s) 92
Psp5II RGGWCCY 1 cut(s) 87
Psp6I CCWGG 1 cut(s) 168
PspEI GGTNACC 1 cut(s) 259
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 4 cut(s) 20, 89, 175, 195
PspPI GGNCC 1 cut(s) 87
PspPPI RGGWCCY 1 cut(s) 87
PsuI RGATCY 1 cut(s) 434
PsyI GACNNNGTC 1 cut(s) 110
SalI GTCGAC 1 cut(s) 95
SatI GCNGC 1 cut(s) 322
Sau3AI GATC 4 cut(s) 118, 355, 406, 434
Sau96I GGNCC 1 cut(s) 87
SchI GAGTC 1 cut(s) 282
ScrFI CCNGG 1 cut(s) 170
SduI GDGCHC 2 cut(s) 178, 198
SetI ASST 6 cut(s) 44, 72, 96, 114, 135, 423
SfcI CTRYAG 1 cut(s) 216
SgrDI CGTCGACG 1 cut(s) 95
SinI GGWCC 1 cut(s) 87
SphI GCATGC 1 cut(s) 328
Sse9I AATT 2 cut(s) 184, 204
SsiI CCGC 1 cut(s) 322
SspMI CTAG 2 cut(s) 2, 422
StyD4I CCNGG 1 cut(s) 168
TaaI ACNGT 1 cut(s) 245
TaiI ACGT 3 cut(s) 96, 114, 135
TaqI TCGA 5 cut(s) 96, 117, 129, 141, 147
TasI AATT 2 cut(s) 184, 204
TauI GCSGC 1 cut(s) 324
TfiI GAWTC 2 cut(s) 232, 253
TscAI CASTG 2 cut(s) 128, 243
TseFI GTSAC 2 cut(s) 259, 278
Tsp45I GTSAC 2 cut(s) 259, 278
TspDTI ATGAA 4 cut(s) 47, 80, 324, 441
TspRI CASTG 2 cut(s) 128, 243
Tth111I GACNNNGTC 1 cut(s) 110
VpaK11BI GGWCC 1 cut(s) 87
XceI RCATGY 1 cut(s) 328
XmiI GTMKAC 1 cut(s) 96
XspI CTAG 2 cut(s) 2, 422
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.