FvH4_2g05400

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000645
Physical Location & Seq
Reverse (-)
4445841 .. 4447253
1413 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05400.t1

Sequence Viewer

Length: 504 bp
ATGAATAACACCCTGTTCCACCACCTCGTCCTTCTTCTTCTGATACCGTTCACCCTAGTATACACCATTACTACCCCCGCCTCATCCTCAGCCAACCAGGCTTACACTGCCCTCACCGAGGACTGCTCGGTGTCCGGCGAGGATGTCCCTATGCCCATACGAAGGGAGGTGTACGATAACGGTCGGATATTCGACATTAGTCACAGATACACCCCTGACATGCCGAGTTACGGCGTTAAGGACGGGCTCGGGGAATTCCTGTCGCTTGGCATGAGCATCAAAAATGGCTCGGACGCCAACGTCTCGGTTGTTTTGGCCACGATCCTCAGCAGGGATAGAGAGGTAACTTGTGCTACCAAAAGCGAAGTAGAGCTCCGGTGTCACCTCGCGGGCGAGGAATTACGAGCAAACTATCTAGTCACCCGTGAATCGATACTCACTGTTGGGGTGAGCAGAGCAACAATTGGGAAAGAGAGGCGAGTCCAGACGGGCTCTGAGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

168

Amino Acids

18.37

Weight (kDa)

5.55

Isoelectric Point (pI)

40.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 60
AccII CGCG 1 cut(s) 389
AciI CCGC 2 cut(s) 78, 389
AclWI GGATC 1 cut(s) 316
AcoI YGGCCR 1 cut(s) 315
AcsI RAATTY 1 cut(s) 254
AcyI GRCGYC 1 cut(s) 294
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 4 cut(s) 118, 162, 230, 331
AjnI CCWGG 1 cut(s) 96
AluBI AGCT 1 cut(s) 373
AluI AGCT 1 cut(s) 373
Alw21I GWGCWC 1 cut(s) 375
Alw26I GTCTC 1 cut(s) 307
AlwI GGATC 1 cut(s) 316
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 1 cut(s) 315
ApoI RAATTY 1 cut(s) 254
AsuHPI GGTGA 5 cut(s) 43, 106, 374, 412, 460
AvaI CYCGRG 1 cut(s) 248
BalI TGGCCA 1 cut(s) 317
BanII GRGCYC 3 cut(s) 249, 375, 494
Bbv12I GWGCWC 1 cut(s) 375
BbvCI CCTCAGC 2 cut(s) 88, 326
BceAI ACGGC 1 cut(s) 247
BciT130I CCWGG 1 cut(s) 98
BcoDI GTCTC 1 cut(s) 307
BfaI CTAG 2 cut(s) 56, 416
BglI GCCNNNNNGGC 1 cut(s) 98
BglII AGATCT 1 cut(s) 498
Bme1390I CCNGG 1 cut(s) 98
BmeT110I CYCGRG 1 cut(s) 248
BmrFI CCNGG 1 cut(s) 98
BmsI GCATC 1 cut(s) 285
BoxI GACNNNNGTC 1 cut(s) 198
BplI GAGNNNNNCTC 2 cut(s) 110, 142
Bpu10I CCTNAGC 2 cut(s) 88, 326
Bsa29I ATCGAT 1 cut(s) 431
BsaHI GRCGYC 1 cut(s) 294
BsaJI CCNNGG 1 cut(s) 117
BsaWI WCCGGW 1 cut(s) 375
Bsc4I CCNNNNNNNGG 4 cut(s) 118, 162, 230, 331
BseBI CCWGG 1 cut(s) 98
BseCI ATCGAT 1 cut(s) 431
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 2 cut(s) 83, 148
BseLI CCNNNNNNNGG 4 cut(s) 118, 162, 230, 331
BseMII CTCAG 3 cut(s) 102, 340, 486
Bsh1236I CGCG 1 cut(s) 389
Bsh1285I CGRYCG 1 cut(s) 184
BshFI GGCC 1 cut(s) 317
BshVI ATCGAT 1 cut(s) 431
BsiEI CGRYCG 1 cut(s) 184
BsiHKAI GWGCWC 1 cut(s) 375
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 2 cut(s) 135, 376
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 4 cut(s) 118, 162, 230, 331
BsmAI GTCTC 1 cut(s) 307
BsmBI CGTCTC 1 cut(s) 307
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 1 cut(s) 317
BsoBI CYCGRG 1 cut(s) 248
Bsp1286I GDGCHC 3 cut(s) 249, 375, 494
Bsp143I GATC 2 cut(s) 321, 498
BspACI CCGC 2 cut(s) 78, 389
BspANI GGCC 1 cut(s) 317
BspCNI CTCAG 3 cut(s) 101, 339, 487
BspDI ATCGAT 1 cut(s) 431
BspFNI CGCG 1 cut(s) 389
BspPI GGATC 1 cut(s) 316
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 2 cut(s) 321, 498
BssNAI GTATAC 1 cut(s) 61
BssNI GRCGYC 1 cut(s) 294
Bst1107I GTATAC 1 cut(s) 61
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 3 cut(s) 48, 182, 442
BstACI GRCGYC 1 cut(s) 294
BstC8I GCNNGC 1 cut(s) 391
BstDEI CTNAG 3 cut(s) 88, 326, 495
BstENI CCTNNNNNAGG 1 cut(s) 116
BstF5I GGATG 2 cut(s) 83, 148
BstFNI CGCG 1 cut(s) 389
BstKTI GATC 2 cut(s) 324, 501
BstMAI GTCTC 1 cut(s) 307
BstMBI GATC 2 cut(s) 321, 498
BstMCI CGRYCG 1 cut(s) 184
BstMWI GCNNNNNNNGC 2 cut(s) 98, 107
BstNI CCWGG 1 cut(s) 98
BstNSI RCATGY 1 cut(s) 223
BstPAI GACNNNNGTC 1 cut(s) 198
BstSCI CCNGG 1 cut(s) 96
BstUI CGCG 1 cut(s) 389
BstX2I RGATCY 1 cut(s) 498
BstYI RGATCY 1 cut(s) 498
BstZ17I GTATAC 1 cut(s) 61
Bsu15I ATCGAT 1 cut(s) 431
BsuRI GGCC 1 cut(s) 317
BsuTUI ATCGAT 1 cut(s) 431
BtsCI GGATG 2 cut(s) 83, 148
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 2 cut(s) 105, 438
Cac8I GCNNGC 1 cut(s) 391
ClaI ATCGAT 1 cut(s) 431
CseI GACGC 1 cut(s) 302
Csp6I GTAC 1 cut(s) 172
CviAII CATG 2 cut(s) 220, 271
CviJI RGCY 7 cut(s) 92, 101, 247, 288, 317, 373, 492
CviKI_1 RGCY 7 cut(s) 92, 101, 247, 288, 317, 373, 492
CviQI GTAC 1 cut(s) 172
DdeI CTNAG 3 cut(s) 88, 326, 495
DpnI GATC 2 cut(s) 323, 500
DpnII GATC 2 cut(s) 321, 498
DrdI GACNNNNNNGTC 1 cut(s) 299
DseDI GACNNNNNNGTC 1 cut(s) 299
EaeI YGGCCR 1 cut(s) 315
Ecl136II GAGCTC 1 cut(s) 373
Eco24I GRGCYC 3 cut(s) 249, 375, 494
Eco53kI GAGCTC 1 cut(s) 373
Eco88I CYCGRG 1 cut(s) 248
EcoICRI GAGCTC 1 cut(s) 373
EcoNI CCTNNNNNAGG 1 cut(s) 116
EcoRI GAATTC 1 cut(s) 254
EcoRII CCWGG 1 cut(s) 96
EcoT38I GRGCYC 3 cut(s) 249, 375, 494
Esp3I CGTCTC 1 cut(s) 307
FaeI CATG 2 cut(s) 223, 274
FaiI YATR 5 cut(s) 61, 152, 158, 221, 272
FaqI GGGAC 1 cut(s) 131
FatI CATG 2 cut(s) 219, 270
FauI CCCGC 2 cut(s) 85, 382
FblI GTMKAC 1 cut(s) 60
FokI GGATG 2 cut(s) 70, 155
FriOI GRGCYC 3 cut(s) 249, 375, 494
FspBI CTAG 2 cut(s) 56, 416
HaeIII GGCC 1 cut(s) 317
HapII CCGG 2 cut(s) 135, 376
HgaI GACGC 1 cut(s) 302
Hin1I GRCGYC 1 cut(s) 294
Hin1II CATG 2 cut(s) 223, 274
HinfI GANTC 2 cut(s) 428, 480
HpaII CCGG 2 cut(s) 135, 376
HphI GGTGA 5 cut(s) 43, 106, 374, 412, 460
Hpy166II GTNNAC 3 cut(s) 51, 61, 172
Hpy188I TCNGA 4 cut(s) 42, 186, 292, 496
Hpy188III TCNNGA 1 cut(s) 484
Hpy8I GTNNAC 3 cut(s) 51, 61, 172
HpyAV CCTTC 2 cut(s) 41, 156
HpyCH4III ACNGT 3 cut(s) 48, 182, 442
HpyCH4IV ACGT 1 cut(s) 300
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 107
HpyF3I CTNAG 3 cut(s) 88, 326, 495
HpySE526I ACGT 1 cut(s) 300
Hsp92I GRCGYC 1 cut(s) 294
Hsp92II CATG 2 cut(s) 223, 274
Kzo9I GATC 2 cut(s) 321, 498
LmnI GCTCC 1 cut(s) 378
LpnPI CCDG 9 cut(s) 26, 83, 110, 148, 228, 272, 316, 389, 497
LweI GCATC 1 cut(s) 285
MaeI CTAG 2 cut(s) 56, 416
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 5 cut(s) 200, 227, 343, 380, 418
MalI GATC 2 cut(s) 323, 500
MboI GATC 2 cut(s) 321, 498
MboII GAAGA 2 cut(s) 26, 29
MfeI CAATTG 1 cut(s) 462
MflI RGATCY 1 cut(s) 498
MhlI GDGCHC 3 cut(s) 249, 375, 494
MlsI TGGCCA 1 cut(s) 317
MluCI AATT 3 cut(s) 254, 398, 462
MluNI TGGCCA 1 cut(s) 317
MlyI GAGTC 1 cut(s) 489
MmeI TCCRAC 1 cut(s) 164
Mox20I TGGCCA 1 cut(s) 317
MscI TGGCCA 1 cut(s) 317
MseI TTAA 1 cut(s) 237
Msp20I TGGCCA 1 cut(s) 317
MspI CCGG 2 cut(s) 135, 376
MspR9I CCNGG 1 cut(s) 98
MunI CAATTG 1 cut(s) 462
MvaI CCWGG 1 cut(s) 98
MvnI CGCG 1 cut(s) 389
MwoI GCNNNNNNNGC 2 cut(s) 98, 107
NdeII GATC 2 cut(s) 321, 498
NlaIII CATG 2 cut(s) 223, 274
NmeAIII GCCGAG 1 cut(s) 249
NmuCI GTSAC 3 cut(s) 200, 380, 418
NspI RCATGY 1 cut(s) 223
PfeI GAWTC 1 cut(s) 428
PleI GAGTC 1 cut(s) 488
PpsI GAGTC 1 cut(s) 488
PshAI GACNNNNGTC 1 cut(s) 198
Psp124BI GAGCTC 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 96
PspGI CCWGG 1 cut(s) 96
PsuI RGATCY 1 cut(s) 498
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
SacI GAGCTC 1 cut(s) 375
SaqAI TTAA 1 cut(s) 237
Sau3AI GATC 2 cut(s) 321, 498
SchI GAGTC 1 cut(s) 489
ScrFI CCNGG 1 cut(s) 98
SduI GDGCHC 3 cut(s) 249, 375, 494
SetI ASST 6 cut(s) 27, 171, 303, 345, 375, 387
SfaNI GCATC 1 cut(s) 285
Sse9I AATT 3 cut(s) 254, 398, 462
SsiI CCGC 2 cut(s) 78, 389
SspMI CTAG 2 cut(s) 56, 416
SstI GAGCTC 1 cut(s) 375
StyD4I CCNGG 1 cut(s) 96
TaaI ACNGT 3 cut(s) 48, 182, 442
TaiI ACGT 1 cut(s) 303
TaqI TCGA 2 cut(s) 192, 431
TasI AATT 3 cut(s) 254, 398, 462
TfiI GAWTC 1 cut(s) 428
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 2 cut(s) 112, 445
TseFI GTSAC 3 cut(s) 200, 380, 418
Tsp45I GTSAC 3 cut(s) 200, 380, 418
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 112, 445
XagI CCTNNNNNAGG 1 cut(s) 116
XapI RAATTY 1 cut(s) 254
XceI RCATGY 1 cut(s) 223
XmiI GTMKAC 1 cut(s) 60
XspI CTAG 2 cut(s) 56, 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.