Rh6DG066900

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000645
Physical Location & Seq
Reverse (-)
6815336 .. 6817154
1819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG066900.1

Sequence Viewer

Length: 444 bp
ATGTGCACGGCCGTTGCCACCGTCACCTCATCCTCTGACTCAGCTTACCCCACAGTCACCGAGGACTGCTCGGTGGGCTCCGGCGAGGCCGTCCCTGTCCCGGTACGTAGAGAGGTGTACGATAACGGTCGAATATTCGACATCAGCCACAGGTACACTGCTGAGATGCCGAGTTTTGCCTCCGAAAACGGGCGACTGGGAAGCGACTTCTTGTGGCTGGGATTCAGCATCAAGAACGGATCGCTGGCAAATGCCTCCATGATGAAGTTCTGTGTCCACTCGGGCACTCACGTCGACGCACCTGGTCACGTCTTCGACCATTACTACCATGCTGGATTTGATGTCGATGCTTTAGACCTCGAAGTGTTAAACGGCCCTGCGCTGGTGGTTGATGTTCCCAGGGATAAGAACACAACTGGTATGTTCCCATTCTGCGATTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

147

Amino Acids

15.9

Weight (kDa)

4.74

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 46 - 135 5.2e-11 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 442
AccI GTMKAC 1 cut(s) 294
AclWI GGATC 1 cut(s) 247
AcoI YGGCCR 1 cut(s) 9
AfaI GTAC 3 cut(s) 105, 119, 155
AfiI CCNNNNNNNGG 3 cut(s) 100, 189, 382
AjiI CACGTC 2 cut(s) 292, 310
AjnI CCWGG 2 cut(s) 301, 398
AluBI AGCT 1 cut(s) 44
AluI AGCT 1 cut(s) 44
Alw21I GWGCWC 1 cut(s) 8
Alw44I GTGCAC 1 cut(s) 4
AlwI GGATC 1 cut(s) 247
Ama87I CYCGRG 1 cut(s) 280
AoxI GGCC 3 cut(s) 9, 87, 373
ApaLI GTGCAC 1 cut(s) 4
AspLEI GCGC 1 cut(s) 382
AspS9I GGNCC 1 cut(s) 374
AsuC2I CCSGG 1 cut(s) 101
AsuHPI GGTGA 2 cut(s) 16, 49
AvaI CYCGRG 1 cut(s) 280
BaeGI GKGCMC 2 cut(s) 8, 287
BanII GRGCYC 1 cut(s) 80
BbsI GAAGAC 1 cut(s) 304
Bbv12I GWGCWC 1 cut(s) 8
BceAI ACGGC 3 cut(s) 24, 74, 388
BcgI CGANNNNNNTGC 2 cut(s) 274, 308
BciT130I CCWGG 2 cut(s) 303, 400
BcnI CCSGG 1 cut(s) 101
Bme1390I CCNGG 3 cut(s) 101, 303, 400
BmeT110I CYCGRG 1 cut(s) 280
BmgBI CACGTC 2 cut(s) 292, 310
BmgT120I GGNCC 1 cut(s) 374
BmiI GGNNCC 1 cut(s) 79
BmrFI CCNGG 3 cut(s) 101, 303, 400
BmrI ACTGGG 1 cut(s) 206
BmsI GCATC 3 cut(s) 156, 237, 337
BmuI ACTGGG 1 cut(s) 206
BpiI GAAGAC 1 cut(s) 304
BplI GAGNNNNNCTC 2 cut(s) 53, 85
BpuMI CCSGG 1 cut(s) 101
BsaAI YACGTR 1 cut(s) 107
BsaJI CCNNGG 3 cut(s) 60, 398, 399
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 189, 382
Bse1I ACTGG 2 cut(s) 201, 421
BseBI CCWGG 2 cut(s) 303, 400
BseDI CCNNGG 3 cut(s) 60, 398, 399
BseGI GGATG 1 cut(s) 29
BseLI CCNNNNNNNGG 3 cut(s) 100, 189, 382
BseMII CTCAG 2 cut(s) 54, 153
BseNI ACTGG 2 cut(s) 201, 421
BseSI GKGCMC 2 cut(s) 8, 287
BseX3I CGGCCG 1 cut(s) 9
BseYI CCCAGC 1 cut(s) 217
Bsh1285I CGRYCG 2 cut(s) 12, 130
BshFI GGCC 3 cut(s) 11, 89, 375
BsiEI CGRYCG 2 cut(s) 12, 130
BsiHKAI GWGCWC 1 cut(s) 8
BsiHKCI CYCGRG 1 cut(s) 280
BsiSI CCGG 2 cut(s) 81, 101
BslFI GGGAC 2 cut(s) 77, 83
BslI CCNNNNNNNGG 3 cut(s) 100, 189, 382
BsmFI GGGAC 2 cut(s) 77, 83
BsnI GGCC 3 cut(s) 11, 89, 375
BsoBI CYCGRG 1 cut(s) 280
Bsp1286I GDGCHC 3 cut(s) 8, 80, 287
Bsp143I GATC 1 cut(s) 239
BspANI GGCC 3 cut(s) 11, 89, 375
BspCNI CTCAG 2 cut(s) 53, 154
BspLI GGNNCC 1 cut(s) 79
BspPI GGATC 1 cut(s) 247
BsrI ACTGG 2 cut(s) 201, 421
BssECI CCNNGG 3 cut(s) 60, 398, 399
BssMI GATC 1 cut(s) 239
Bst2UI CCWGG 2 cut(s) 303, 400
Bst4CI ACNGT 3 cut(s) 22, 55, 128
BstBAI YACGTR 1 cut(s) 107
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 2 cut(s) 40, 162
BstF5I GGATG 1 cut(s) 29
BstHHI GCGC 1 cut(s) 382
BstKTI GATC 1 cut(s) 242
BstMBI GATC 1 cut(s) 239
BstMCI CGRYCG 2 cut(s) 12, 130
BstMWI GCNNNNNNNGC 1 cut(s) 75
BstNI CCWGG 2 cut(s) 303, 400
BstSCI CCNGG 3 cut(s) 99, 301, 398
BstSLI GKGCMC 2 cut(s) 8, 287
BstSNI TACGTA 1 cut(s) 107
BstV2I GAAGAC 1 cut(s) 304
BstZI CGGCCG 1 cut(s) 9
BsuRI GGCC 3 cut(s) 11, 89, 375
BtrI CACGTC 2 cut(s) 292, 310
BtsCI GGATG 1 cut(s) 29
BtsI GCAGTG 1 cut(s) 156
BtsIMutI CAGTG 1 cut(s) 156
Cac8I GCNNGC 1 cut(s) 246
CfoI GCGC 1 cut(s) 382
Cfr13I GGNCC 1 cut(s) 374
CseI GACGC 1 cut(s) 305
CsiI ACCWGGT 1 cut(s) 301
Csp6I GTAC 3 cut(s) 104, 118, 154
CviAII CATG 2 cut(s) 259, 329
CviJI RGCY 7 cut(s) 11, 44, 78, 89, 147, 217, 375
CviKI_1 RGCY 7 cut(s) 11, 44, 78, 89, 147, 217, 375
CviQI GTAC 3 cut(s) 104, 118, 154
DdeI CTNAG 2 cut(s) 40, 162
DpnI GATC 1 cut(s) 241
DpnII GATC 1 cut(s) 239
EaeI YGGCCR 1 cut(s) 9
EagI CGGCCG 1 cut(s) 9
EclXI CGGCCG 1 cut(s) 9
Eco105I TACGTA 1 cut(s) 107
Eco24I GRGCYC 1 cut(s) 80
Eco52I CGGCCG 1 cut(s) 9
Eco88I CYCGRG 1 cut(s) 280
EcoRII CCWGG 2 cut(s) 301, 398
EcoT38I GRGCYC 1 cut(s) 80
FaeI CATG 2 cut(s) 262, 332
FaiI YATR 4 cut(s) 260, 330, 422, 442
FaqI GGGAC 2 cut(s) 77, 83
FatI CATG 2 cut(s) 258, 328
FblI GTMKAC 1 cut(s) 294
FokI GGATG 1 cut(s) 16
FriOI GRGCYC 1 cut(s) 80
GlaI GCGC 1 cut(s) 381
GsaI CCCAGC 1 cut(s) 221
HaeIII GGCC 3 cut(s) 11, 89, 375
HapII CCGG 2 cut(s) 81, 101
HgaI GACGC 1 cut(s) 305
HhaI GCGC 1 cut(s) 382
Hin1II CATG 2 cut(s) 262, 332
Hin6I GCGC 1 cut(s) 380
HinP1I GCGC 1 cut(s) 380
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 2 cut(s) 38, 222
HpaII CCGG 2 cut(s) 81, 101
HphI GGTGA 2 cut(s) 16, 49
Hpy166II GTNNAC 5 cut(s) 6, 118, 156, 277, 295
Hpy188I TCNGA 2 cut(s) 37, 184
Hpy188III TCNNGA 1 cut(s) 232
Hpy8I GTNNAC 5 cut(s) 6, 118, 156, 277, 295
Hpy99I CGWCG 2 cut(s) 296, 299
HpyCH4III ACNGT 3 cut(s) 22, 55, 128
HpyCH4IV ACGT 3 cut(s) 106, 291, 309
HpyCH4V TGCA 1 cut(s) 6
HpyF10VI GCNNNNNNNGC 1 cut(s) 75
HpyF3I CTNAG 2 cut(s) 40, 162
HpySE526I ACGT 3 cut(s) 106, 291, 309
Hsp92II CATG 2 cut(s) 262, 332
HspAI GCGC 1 cut(s) 380
Kzo9I GATC 1 cut(s) 239
LmnI GCTCC 1 cut(s) 83
LweI GCATC 3 cut(s) 156, 237, 337
MabI ACCWGGT 1 cut(s) 301
MaeII ACGT 3 cut(s) 106, 291, 309
MaeIII GTNAC 3 cut(s) 22, 55, 305
MalI GATC 1 cut(s) 241
MboI GATC 1 cut(s) 239
MboII GAAGA 1 cut(s) 304
MhlI GDGCHC 3 cut(s) 8, 80, 287
MlyI GAGTC 1 cut(s) 32
MnlI CCTC 8 cut(s) 37, 43, 55, 79, 106, 190, 265, 368
MseI TTAA 1 cut(s) 368
MspI CCGG 2 cut(s) 81, 101
MspR9I CCNGG 3 cut(s) 101, 303, 400
MvaI CCWGG 2 cut(s) 303, 400
MwoI GCNNNNNNNGC 1 cut(s) 75
NciI CCSGG 1 cut(s) 101
NdeII GATC 1 cut(s) 239
NlaIII CATG 2 cut(s) 262, 332
NlaIV GGNNCC 1 cut(s) 79
NmeAIII GCCGAG 1 cut(s) 195
NmuCI GTSAC 3 cut(s) 22, 55, 305
PasI CCCWGGG 1 cut(s) 399
PfeI GAWTC 1 cut(s) 222
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
Ppu21I YACGTR 1 cut(s) 107
PsiI TTATAA 1 cut(s) 442
Psp6I CCWGG 2 cut(s) 301, 398
PspFI CCCAGC 1 cut(s) 217
PspGI CCWGG 2 cut(s) 301, 398
PspN4I GGNNCC 1 cut(s) 79
PspPI GGNCC 1 cut(s) 374
RsaI GTAC 3 cut(s) 105, 119, 155
RsaNI GTAC 3 cut(s) 104, 118, 154
SalI GTCGAC 1 cut(s) 293
SaqAI TTAA 1 cut(s) 368
Sau3AI GATC 1 cut(s) 239
Sau96I GGNCC 1 cut(s) 374
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 3 cut(s) 101, 303, 400
SduI GDGCHC 3 cut(s) 8, 80, 287
SetI ASST 9 cut(s) 29, 46, 109, 117, 155, 294, 304, 312, 360
SexAI ACCWGGT 1 cut(s) 301
SfaNI GCATC 3 cut(s) 156, 237, 337
SgrDI CGTCGACG 1 cut(s) 293
SnaBI TACGTA 1 cut(s) 107
SspI AATATT 1 cut(s) 135
StyD4I CCNGG 3 cut(s) 99, 301, 398
TaaI ACNGT 3 cut(s) 22, 55, 128
TaiI ACGT 3 cut(s) 109, 294, 312
TaqI TCGA 6 cut(s) 130, 138, 294, 315, 345, 360
TfiI GAWTC 1 cut(s) 222
Tru1I TTAA 1 cut(s) 368
Tru9I TTAA 1 cut(s) 368
TscAI CASTG 1 cut(s) 163
TseFI GTSAC 3 cut(s) 22, 55, 305
Tsp45I GTSAC 3 cut(s) 22, 55, 305
TspDTI ATGAA 1 cut(s) 278
TspGWI ACGGA 1 cut(s) 252
TspRI CASTG 1 cut(s) 163
VneI GTGCAC 1 cut(s) 4
XmiI GTMKAC 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.