FvH4_2g05360

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000645
Physical Location & Seq
Forward (+)
4420576 .. 4425930
5355 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05360.t2

Sequence Viewer

Length: 828 bp
ATGAAGACCTCACTGCTCCTCCTCCTCTGCTCCCTTTCCTTCACTCTCACCACTCTCTCACACGCCACCTCCGCCTACCCTTCCATTCCCGGAACCTCCCCTTCCGACTGTTCTTTCTCCAACGACGACACCAACCCTTTAGTTCCCCCTCGCCGTGAAGTATACTCAAATGGCCGAATATTCGACATCACCCACCGTATCACAACGGCCACGCCGTCGTATGACTCCCCGGACGGCGTGCTGGGTCAGTTCCTGTGGCTCCCACGCAGCATGAAGAACGGCTCCCTTGCGAACATGTCAGTTTTCAAGCTGCCTACTCACACCGGCACGCATGTTGATTCTCCCGGACACTTCTTCGATCACTACCTCGATGCCGGCTTCGATGTTGATACGCTTGACTTGGAGGTCCTTAATGGTCCGGCATTGTTAGTTGATGTTCCGAGGGACAAGAACATAACTGCTGAAGTGATGAAGTCCTTAAATATTCCGAAGGGAGTACGTCGTGTGCTTTTCAGAACATTAAATACTGACAGACGGCTTATGTTCAAAAGTCAGTTTGACACAAGCTATGTGGGATTTGTGAAGGATGGAGCCAAGTGGTTGGTAGAGAACACTGACATCAAACTTGTTGGAATTGATTACTTATCTGTTGCTGCATTTGATGATTTGATTCCATCCCATCTTGTTTTTCTAGAAAGCAGGGAAATCATCCTTGTGGAAGCTCTAAAACTCGATGACATCCAACCGGGAATATATTCTGTCCATTGCTTACCTTTGAGGTTGCAAGGTGCAGAAGGATCACCAATCAGATGCATTCTTATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

276

Amino Acids

30.51

Weight (kDa)

5.88

Isoelectric Point (pI)

36.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 61 - 173 2e-12 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 404
AccI GTMKAC 1 cut(s) 162
AciI CCGC 1 cut(s) 72
AclWI GGATC 1 cut(s) 805
AcoI YGGCCR 2 cut(s) 172, 207
AcuI CTGAAG 1 cut(s) 483
AfaI GTAC 1 cut(s) 498
AflIII ACRYGT 1 cut(s) 294
AgsI TTSAA 2 cut(s) 307, 547
AluBI AGCT 3 cut(s) 310, 567, 722
AluI AGCT 3 cut(s) 310, 567, 722
AlwI GGATC 1 cut(s) 805
AlwNI CAGNNNCTG 1 cut(s) 253
AoxI GGCC 2 cut(s) 172, 207
ApeKI GCWGC 3 cut(s) 267, 310, 653
Asp700I GAANNNNTTC 1 cut(s) 754
AspS9I GGNCC 2 cut(s) 406, 416
AsuC2I CCSGG 4 cut(s) 90, 230, 345, 747
AsuHPI GGTGA 3 cut(s) 40, 181, 792
AvaII GGWCC 2 cut(s) 406, 416
BbsI GAAGAC 1 cut(s) 11
BbvI GCAGC 3 cut(s) 279, 297, 640
BccI CCATC 3 cut(s) 581, 682, 687
BceAI ACGGC 6 cut(s) 138, 199, 222, 250, 295, 551
BcnI CCSGG 4 cut(s) 90, 230, 345, 747
BfaI CTAG 1 cut(s) 692
BisI GCNGC 3 cut(s) 268, 311, 654
BlsI GCNGC 3 cut(s) 269, 312, 655
Bme1390I CCNGG 4 cut(s) 90, 230, 345, 747
Bme18I GGWCC 2 cut(s) 406, 416
BmgT120I GGNCC 2 cut(s) 406, 416
BmiI GGNNCC 4 cut(s) 94, 260, 283, 592
BmrFI CCNGG 4 cut(s) 90, 230, 345, 747
BmsI GCATC 2 cut(s) 361, 800
BpiI GAAGAC 1 cut(s) 11
BpuMI CCSGG 4 cut(s) 90, 230, 345, 747
BsaJI CCNNGG 2 cut(s) 228, 440
BsaXI ACNNNNNCTCC 3 cut(s) 33, 53, 83
Bse118I RCCGGY 2 cut(s) 323, 374
Bse3DI GCAATG 1 cut(s) 763
BseDI CCNNGG 2 cut(s) 228, 440
BseGI GGATG 4 cut(s) 592, 674, 708, 738
BseMI GCAATG 1 cut(s) 763
BseRI GAGGAG 3 cut(s) 8, 11, 14
BseXI GCAGC 3 cut(s) 279, 297, 640
BseYI CCCAGC 1 cut(s) 241
BsgI GTGCAG 1 cut(s) 810
BshFI GGCC 2 cut(s) 174, 209
BsiSI CCGG 7 cut(s) 90, 230, 324, 345, 375, 419, 746
BslFI GGGAC 1 cut(s) 458
BsmFI GGGAC 1 cut(s) 458
BsmI GAATGC 1 cut(s) 813
BsnI GGCC 2 cut(s) 174, 209
Bsp143I GATC 2 cut(s) 358, 797
BspACI CCGC 1 cut(s) 72
BspANI GGCC 2 cut(s) 174, 209
BspLI GGNNCC 4 cut(s) 94, 260, 283, 592
BspPI GGATC 1 cut(s) 805
BsrDI GCAATG 1 cut(s) 763
BsrFI RCCGGY 2 cut(s) 323, 374
BssAI RCCGGY 2 cut(s) 323, 374
BssECI CCNNGG 2 cut(s) 228, 440
BssMI GATC 2 cut(s) 358, 797
BssNAI GTATAC 1 cut(s) 163
Bst1107I GTATAC 1 cut(s) 163
Bst4CI ACNGT 2 cut(s) 110, 197
BstC8I GCNNGC 3 cut(s) 239, 329, 376
BstF5I GGATG 4 cut(s) 592, 674, 708, 738
BstKTI GATC 2 cut(s) 361, 800
BstMBI GATC 2 cut(s) 358, 797
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstNSI RCATGY 2 cut(s) 298, 335
BstSCI CCNGG 4 cut(s) 88, 228, 343, 745
BstV1I GCAGC 3 cut(s) 279, 297, 640
BstV2I GAAGAC 1 cut(s) 11
BstXI CCANNNNNNTGG 1 cut(s) 601
BstZ17I GTATAC 1 cut(s) 163
BsuRI GGCC 2 cut(s) 174, 209
BtsCI GGATG 4 cut(s) 592, 674, 708, 738
BtsI GCAGTG 1 cut(s) 11
BtsIMutI CAGTG 2 cut(s) 11, 612
Cac8I GCNNGC 3 cut(s) 239, 329, 376
CaiI CAGNNNCTG 1 cut(s) 253
Cfr10I RCCGGY 2 cut(s) 323, 374
Cfr13I GGNCC 2 cut(s) 406, 416
Csp6I GTAC 1 cut(s) 497
CspCI CAANNNNNGTGG 2 cut(s) 552, 587
CviAII CATG 3 cut(s) 271, 295, 332
CviQI GTAC 1 cut(s) 497
DpnI GATC 2 cut(s) 360, 799
DpnII GATC 2 cut(s) 358, 797
DrdI GACNNNNNNGTC 1 cut(s) 404
DseDI GACNNNNNNGTC 1 cut(s) 404
EaeI YGGCCR 2 cut(s) 172, 207
EciI GGCGGA 1 cut(s) 61
Eco47I GGWCC 2 cut(s) 406, 416
Eco57I CTGAAG 1 cut(s) 483
EcoO109I RGGNCCY 1 cut(s) 406
EcoT22I ATGCAT 1 cut(s) 815
FaeI CATG 3 cut(s) 274, 298, 335
FaiI YATR 9 cut(s) 163, 222, 272, 296, 333, 455, 542, 570, 754
FaqI GGGAC 1 cut(s) 458
FatI CATG 3 cut(s) 270, 294, 331
FblI GTMKAC 1 cut(s) 162
Fnu4HI GCNGC 3 cut(s) 268, 311, 654
FokI GGATG 4 cut(s) 599, 661, 695, 725
Fsp4HI GCNGC 3 cut(s) 268, 311, 654
FspBI CTAG 1 cut(s) 692
GluI GCNGC 3 cut(s) 268, 311, 654
GsaI CCCAGC 1 cut(s) 245
HaeIII GGCC 2 cut(s) 174, 209
HapII CCGG 7 cut(s) 90, 230, 324, 345, 375, 419, 746
Hin1II CATG 3 cut(s) 274, 298, 335
HinfI GANTC 3 cut(s) 224, 338, 670
HpaII CCGG 7 cut(s) 90, 230, 324, 345, 375, 419, 746
HphI GGTGA 3 cut(s) 40, 181, 792
Hpy166II GTNNAC 1 cut(s) 163
Hpy188I TCNGA 5 cut(s) 106, 441, 489, 515, 809
Hpy188III TCNNGA 1 cut(s) 692
Hpy8I GTNNAC 1 cut(s) 163
Hpy99I CGWCG 3 cut(s) 128, 220, 504
HpyAV CCTTC 6 cut(s) 49, 90, 111, 484, 577, 788
HpyCH4III ACNGT 2 cut(s) 110, 197
HpyCH4IV ACGT 1 cut(s) 499
HpyCH4V TGCA 4 cut(s) 656, 784, 791, 813
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpySE526I ACGT 1 cut(s) 499
Hsp92II CATG 3 cut(s) 274, 298, 335
KroI GCCGGC 1 cut(s) 374
KroNI GCCGGC 1 cut(s) 376
Kzo9I GATC 2 cut(s) 358, 797
LmnI GCTCC 5 cut(s) 21, 35, 264, 287, 590
Lsp1109I GCAGC 3 cut(s) 279, 297, 640
LweI GCATC 2 cut(s) 361, 800
MaeI CTAG 1 cut(s) 692
MaeII ACGT 1 cut(s) 499
MalI GATC 2 cut(s) 360, 799
MboI GATC 2 cut(s) 358, 797
MboII GAAGA 3 cut(s) 16, 286, 346
MluCI AATT 1 cut(s) 633
MlyI GAGTC 1 cut(s) 218
MmeI TCCRAC 4 cut(s) 129, 144, 610, 766
Mph1103I ATGCAT 1 cut(s) 815
MroNI GCCGGC 1 cut(s) 374
MroXI GAANNNNTTC 1 cut(s) 754
MseI TTAA 3 cut(s) 411, 479, 521
MslI CAYNNNNRTG 1 cut(s) 713
MspI CCGG 7 cut(s) 90, 230, 324, 345, 375, 419, 746
MspR9I CCNGG 4 cut(s) 90, 230, 345, 747
Mva1269I GAATGC 1 cut(s) 813
MwoI GCNNNNNNNGC 1 cut(s) 71
NaeI GCCGGC 1 cut(s) 376
NciI CCSGG 4 cut(s) 90, 230, 345, 747
NdeII GATC 2 cut(s) 358, 797
NgoMIV GCCGGC 1 cut(s) 374
NlaIII CATG 3 cut(s) 274, 298, 335
NlaIV GGNNCC 4 cut(s) 94, 260, 283, 592
NsiI ATGCAT 1 cut(s) 815
NspI RCATGY 2 cut(s) 298, 335
PciI ACATGT 1 cut(s) 294
PcsI WCGNNNNNNNCGW 1 cut(s) 212
PctI GAATGC 1 cut(s) 813
PdiI GCCGGC 1 cut(s) 376
PdmI GAANNNNTTC 1 cut(s) 754
PfeI GAWTC 2 cut(s) 338, 670
PfoI TCCNGGA 2 cut(s) 88, 343
PkrI GCNGC 3 cut(s) 269, 312, 655
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
PpuMI RGGWCCY 1 cut(s) 406
PscI ACATGT 1 cut(s) 294
Psp5II RGGWCCY 1 cut(s) 406
PspFI CCCAGC 1 cut(s) 241
PspN4I GGNNCC 4 cut(s) 94, 260, 283, 592
PspPI GGNCC 2 cut(s) 406, 416
PspPPI RGGWCCY 1 cut(s) 406
PsrI GAACNNNNNNTAC 2 cut(s) 508, 540
PstNI CAGNNNCTG 1 cut(s) 253
RsaI GTAC 1 cut(s) 498
RsaNI GTAC 1 cut(s) 497
RseI CAYNNNNRTG 1 cut(s) 713
SaqAI TTAA 3 cut(s) 411, 479, 521
SatI GCNGC 3 cut(s) 268, 311, 654
Sau3AI GATC 2 cut(s) 358, 797
Sau96I GGNCC 2 cut(s) 406, 416
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 4 cut(s) 90, 230, 345, 747
SfaNI GCATC 2 cut(s) 361, 800
SinI GGWCC 2 cut(s) 406, 416
SmiMI CAYNNNNRTG 1 cut(s) 713
Sse9I AATT 1 cut(s) 633
SsiI CCGC 1 cut(s) 72
SspI AATATT 2 cut(s) 180, 484
SspMI CTAG 1 cut(s) 692
StyD4I CCNGG 4 cut(s) 88, 228, 343, 745
TaaI ACNGT 2 cut(s) 110, 197
TaiI ACGT 1 cut(s) 502
TaqI TCGA 5 cut(s) 183, 357, 369, 381, 732
TasI AATT 1 cut(s) 633
TfiI GAWTC 2 cut(s) 338, 670
Tru1I TTAA 3 cut(s) 411, 479, 521
Tru9I TTAA 3 cut(s) 411, 479, 521
TscAI CASTG 2 cut(s) 18, 619
TseI GCWGC 3 cut(s) 267, 310, 653
TspDTI ATGAA 3 cut(s) 17, 287, 485
TspRI CASTG 2 cut(s) 18, 619
VpaK11BI GGWCC 2 cut(s) 406, 416
XbaI TCTAGA 1 cut(s) 691
XceI RCATGY 2 cut(s) 298, 335
XmiI GTMKAC 1 cut(s) 162
XmnI GAANNNNTTC 1 cut(s) 754
XspI CTAG 1 cut(s) 692
Zsp2I ATGCAT 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.