MD05G1087600.v1.1

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000645
Physical Location & Seq
Reverse (-)
18754117 .. 18757821
3705 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1087600.v1.1.491

Sequence Viewer

Length: 954 bp
ATGATGCCTTCTATTGATTTCAAATTTAGAGAGAACATTTTAGCTAAAAAAAAACCGTCCCTTTTATCAGTGACTAAGCTGTGTCTTCTAGTCGTAAAGATCACCCGCCCACTTCTCATCCAACCTCCTCCCACTGCCACACGCACCACCATGAAGCCAATACTGCTCCTCCTCCTACTCTCCTCCCTCTCCTACTCCGTCGCCACCACAGCCTATCCTTCTATTCCGGGCACCACCTCCGTCGAATACGCCGCCGAAACCGACACCCTGATCCCTCCTCGCCGTGAAGTTTACGGCAATGGACGAATCTTCGACATCAGCCACCGGTACAGGACCACCATGCCTGCCTTTGAAACCAACGACGGCGTCGGCCAGTTCCTTTGGCTCCCCAACAGCATGAAGAACGGCTCCATCGCCAACAACTCTGAGATGAAGCTGCCCACTCACACTGGTACCCACGTCGATGCCCCCGGCCACGTCTTCGATCACTACTTCGACGCCGGCTTCGATGTCGATACCCTTGACTTGGATGTGCTTAATGGTCCTGCATTGCTAGTTGACGTTCCAAGGGATAAGAACATAACCGCTGAGGTTATGAAGTCCTTACATATTCCCAAGGGGGTATGTCGTGTGCTGTTCAGAACATTAAACACAGACAGACGGCTTATGTTCCAAAAGGAGTTTGACAGCAGCTATGTGGGATTTATGAGGGATGGAGCACAGTGGTTGGTGGAGAACACTGACATTAAACTTGTCGGAATTGATTACTTATCCGTTGCTGCATATGATGATTTGATTCCATCCCACCTTGTTTTTCTAGAAGGCAGGGAAATCATTCTTGTTGAAGCCTTAAAACTGGATGACGTGGAACCAGGAATATATTCTGTCCACTGCTTACCTTTGAGGTTGCCGGGTGCAGAAGGGTCACCGATAAGATGCATTCTTATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

318

Amino Acids

35.37

Weight (kDa)

6.25

Isoelectric Point (pI)

31.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 104 - 259 1.3e-21 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 452
AccB1I GGYRCC 2 cut(s) 230, 452
AciI CCGC 3 cut(s) 106, 252, 585
AclWI GGATC 1 cut(s) 265
AcoI YGGCCR 2 cut(s) 370, 472
AcsI RAATTY 1 cut(s) 23
AcyI GRCGYC 2 cut(s) 366, 498
AfaI GTAC 2 cut(s) 329, 454
AfiI CCNNNNNNNGG 1 cut(s) 526
AgeI ACCGGT 1 cut(s) 324
AgsI TTSAA 3 cut(s) 22, 353, 845
AjiI CACGTC 3 cut(s) 460, 478, 865
AjnI CCWGG 1 cut(s) 871
AluBI AGCT 4 cut(s) 44, 79, 436, 693
AluI AGCT 4 cut(s) 44, 79, 436, 693
Alw21I GWGCWC 1 cut(s) 721
AlwI GGATC 1 cut(s) 265
AoxI GGCC 2 cut(s) 370, 472
ApeKI GCWGC 3 cut(s) 436, 690, 779
ApoI RAATTY 1 cut(s) 23
ArsI GACNNNNNNTTYG 2 cut(s) 488, 520
AsiGI ACCGGT 1 cut(s) 324
Asp700I GAANNNNTTC 2 cut(s) 834, 880
Asp718I GGTACC 1 cut(s) 452
AspS9I GGNCC 2 cut(s) 333, 542
AsuC2I CCSGG 3 cut(s) 228, 471, 912
AsuHPI GGTGA 2 cut(s) 94, 918
AvaII GGWCC 2 cut(s) 333, 542
BaeGI GKGCMC 1 cut(s) 233
BaeI ACNNNNGTAYC 4 cut(s) 436, 444, 469, 477
BanI GGYRCC 2 cut(s) 230, 452
BbsI GAAGAC 2 cut(s) 77, 472
Bbv12I GWGCWC 1 cut(s) 721
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 3 cut(s) 423, 702, 766
BccI CCATC 3 cut(s) 419, 707, 808
BceAI ACGGC 5 cut(s) 267, 310, 379, 421, 677
BciT130I CCWGG 1 cut(s) 873
BcnI CCSGG 3 cut(s) 228, 471, 912
BfaI CTAG 3 cut(s) 89, 554, 818
BisI GCNGC 4 cut(s) 252, 437, 691, 780
BlsI GCNGC 4 cut(s) 253, 438, 692, 781
Bme1390I CCNGG 4 cut(s) 228, 471, 873, 912
Bme18I GGWCC 2 cut(s) 333, 542
BmgBI CACGTC 3 cut(s) 460, 478, 865
BmgT120I GGNCC 2 cut(s) 333, 542
BmiI GGNNCC 5 cut(s) 232, 386, 409, 454, 870
BmrFI CCNGG 4 cut(s) 228, 471, 873, 912
BmsI GCATC 2 cut(s) 454, 926
BpiI GAAGAC 2 cut(s) 77, 472
Bpu10I CCTNAGC 1 cut(s) 588
BpuMI CCSGG 3 cut(s) 228, 471, 912
BsaHI GRCGYC 2 cut(s) 366, 498
BsaJI CCNNGG 3 cut(s) 469, 566, 615
BsaWI WCCGGW 1 cut(s) 324
BsaXI ACNNNNNCTCC 2 cut(s) 153, 183
Bsc4I CCNNNNNNNGG 1 cut(s) 526
Bse118I RCCGGY 2 cut(s) 324, 500
Bse1I ACTGG 3 cut(s) 373, 454, 861
Bse3DI GCAATG 2 cut(s) 304, 548
BseBI CCWGG 1 cut(s) 873
BseDI CCNNGG 3 cut(s) 469, 566, 615
BseGI GGATG 5 cut(s) 117, 535, 718, 800, 865
BseLI CCNNNNNNNGG 1 cut(s) 526
BseMI GCAATG 2 cut(s) 304, 548
BseMII CTCAG 2 cut(s) 417, 579
BseNI ACTGG 3 cut(s) 373, 454, 861
BseRI GAGGAG 5 cut(s) 117, 158, 161, 172, 267
BseSI GKGCMC 1 cut(s) 233
BseXI GCAGC 3 cut(s) 423, 702, 766
BsgI GTGCAG 1 cut(s) 936
BshFI GGCC 2 cut(s) 372, 474
BshNI GGYRCC 2 cut(s) 230, 452
BshTI ACCGGT 1 cut(s) 324
BsiHKAI GWGCWC 1 cut(s) 721
BsiSI CCGG 5 cut(s) 227, 325, 471, 501, 911
BslFI GGGAC 1 cut(s) 43
BslI CCNNNNNNNGG 1 cut(s) 526
BsmFI GGGAC 1 cut(s) 43
BsmI GAATGC 1 cut(s) 939
BsnI GGCC 2 cut(s) 372, 474
Bsp1286I GDGCHC 2 cut(s) 233, 721
Bsp143I GATC 3 cut(s) 99, 270, 484
BspACI CCGC 3 cut(s) 106, 252, 585
BspANI GGCC 2 cut(s) 372, 474
BspCNI CTCAG 2 cut(s) 418, 580
BspLI GGNNCC 5 cut(s) 232, 386, 409, 454, 870
BspPI GGATC 1 cut(s) 265
BspT107I GGYRCC 2 cut(s) 230, 452
BsrDI GCAATG 2 cut(s) 304, 548
BsrFI RCCGGY 2 cut(s) 324, 500
BsrI ACTGG 3 cut(s) 373, 454, 861
BssAI RCCGGY 2 cut(s) 324, 500
BssECI CCNNGG 3 cut(s) 469, 566, 615
BssMI GATC 3 cut(s) 99, 270, 484
BssNI GRCGYC 2 cut(s) 366, 498
BssT1I CCWWGG 2 cut(s) 566, 615
Bst2UI CCWGG 1 cut(s) 873
Bst4CI ACNGT 2 cut(s) 57, 723
BstACI GRCGYC 2 cut(s) 366, 498
BstC8I GCNNGC 2 cut(s) 345, 502
BstDEI CTNAG 3 cut(s) 75, 426, 588
BstEII GGTNACC 1 cut(s) 924
BstF5I GGATG 5 cut(s) 117, 535, 718, 800, 865
BstKTI GATC 3 cut(s) 102, 273, 487
BstMBI GATC 3 cut(s) 99, 270, 484
BstMWI GCNNNNNNNGC 2 cut(s) 163, 209
BstNI CCWGG 1 cut(s) 873
BstPI GGTNACC 1 cut(s) 924
BstSCI CCNGG 4 cut(s) 226, 469, 871, 910
BstSLI GKGCMC 1 cut(s) 233
BstV1I GCAGC 3 cut(s) 423, 702, 766
BstV2I GAAGAC 2 cut(s) 77, 472
BsuRI GGCC 2 cut(s) 372, 474
BtgZI GCGATG 1 cut(s) 397
BtrI CACGTC 3 cut(s) 460, 478, 865
BtsCI GGATG 5 cut(s) 117, 535, 718, 800, 865
BtsI GCAGTG 2 cut(s) 132, 889
BtsIMutI CAGTG 6 cut(s) 75, 132, 447, 728, 738, 889
Cac8I GCNNGC 2 cut(s) 345, 502
Cfr10I RCCGGY 2 cut(s) 324, 500
Cfr13I GGNCC 2 cut(s) 333, 542
CseI GACGC 2 cut(s) 355, 506
Csp6I GTAC 2 cut(s) 328, 453
CspAI ACCGGT 1 cut(s) 324
CviAII CATG 3 cut(s) 151, 340, 397
CviQI GTAC 2 cut(s) 328, 453
DdeI CTNAG 3 cut(s) 75, 426, 588
DpnI GATC 3 cut(s) 101, 272, 486
DpnII GATC 3 cut(s) 99, 270, 484
EaeI YGGCCR 2 cut(s) 370, 472
Eco130I CCWWGG 2 cut(s) 566, 615
Eco47I GGWCC 2 cut(s) 333, 542
Eco91I GGTNACC 1 cut(s) 924
EcoO65I GGTNACC 1 cut(s) 924
EcoRII CCWGG 1 cut(s) 871
EcoT14I CCWWGG 2 cut(s) 566, 615
EcoT22I ATGCAT 1 cut(s) 941
ErhI CCWWGG 2 cut(s) 566, 615
FaeI CATG 3 cut(s) 154, 343, 400
FaqI GGGAC 1 cut(s) 43
FatI CATG 3 cut(s) 150, 339, 396
FauI CCCGC 1 cut(s) 113
FauNDI CATATG 1 cut(s) 784
Fnu4HI GCNGC 4 cut(s) 252, 437, 691, 780
FokI GGATG 5 cut(s) 104, 542, 725, 787, 872
Fsp4HI GCNGC 4 cut(s) 252, 437, 691, 780
FspBI CTAG 3 cut(s) 89, 554, 818
GluI GCNGC 4 cut(s) 252, 437, 691, 780
HaeIII GGCC 2 cut(s) 372, 474
HapII CCGG 5 cut(s) 227, 325, 471, 501, 911
HgaI GACGC 2 cut(s) 355, 506
Hin1I GRCGYC 2 cut(s) 366, 498
Hin1II CATG 3 cut(s) 154, 343, 400
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HinfI GANTC 2 cut(s) 306, 796
HpaII CCGG 5 cut(s) 227, 325, 471, 501, 911
HphI GGTGA 2 cut(s) 94, 918
Hpy166II GTNNAC 3 cut(s) 292, 559, 889
Hpy188I TCNGA 3 cut(s) 427, 641, 758
Hpy188III TCNNGA 1 cut(s) 818
Hpy8I GTNNAC 3 cut(s) 292, 559, 889
Hpy99I CGWCG 6 cut(s) 203, 245, 365, 371, 464, 500
HpyAV CCTTC 4 cut(s) 18, 228, 815, 914
HpyCH4III ACNGT 2 cut(s) 57, 723
HpyCH4IV ACGT 4 cut(s) 459, 477, 561, 864
HpyCH4V TGCA 4 cut(s) 548, 782, 917, 939
HpyF10VI GCNNNNNNNGC 2 cut(s) 163, 209
HpyF3I CTNAG 3 cut(s) 75, 426, 588
HpySE526I ACGT 4 cut(s) 459, 477, 561, 864
Hsp92I GRCGYC 2 cut(s) 366, 498
Hsp92II CATG 3 cut(s) 154, 343, 400
KpnI GGTACC 1 cut(s) 456
KroI GCCGGC 1 cut(s) 500
KroNI GCCGGC 1 cut(s) 502
Kzo9I GATC 3 cut(s) 99, 270, 484
LmnI GCTCC 4 cut(s) 171, 390, 413, 716
Lsp1109I GCAGC 3 cut(s) 423, 702, 766
LweI GCATC 2 cut(s) 454, 926
MaeI CTAG 3 cut(s) 89, 554, 818
MaeII ACGT 4 cut(s) 459, 477, 561, 864
MaeIII GTNAC 2 cut(s) 70, 924
MalI GATC 3 cut(s) 101, 272, 486
MboI GATC 3 cut(s) 99, 270, 484
MboII GAAGA 4 cut(s) 77, 301, 412, 472
MhlI GDGCHC 2 cut(s) 233, 721
MluCI AATT 2 cut(s) 23, 759
MmeI TCCRAC 2 cut(s) 145, 736
Mph1103I ATGCAT 1 cut(s) 941
MroNI GCCGGC 1 cut(s) 500
MroXI GAANNNNTTC 2 cut(s) 834, 880
MseI TTAA 4 cut(s) 537, 647, 747, 851
MslI CAYNNNNRTG 2 cut(s) 149, 462
MspA1I CMGCKG 1 cut(s) 587
MspI CCGG 5 cut(s) 227, 325, 471, 501, 911
MspR9I CCNGG 4 cut(s) 228, 471, 873, 912
Mva1269I GAATGC 1 cut(s) 939
MvaI CCWGG 1 cut(s) 873
MwoI GCNNNNNNNGC 2 cut(s) 163, 209
NaeI GCCGGC 1 cut(s) 502
NciI CCSGG 3 cut(s) 228, 471, 912
NdeI CATATG 1 cut(s) 784
NdeII GATC 3 cut(s) 99, 270, 484
NgoMIV GCCGGC 1 cut(s) 500
NlaIII CATG 3 cut(s) 154, 343, 400
NlaIV GGNNCC 5 cut(s) 232, 386, 409, 454, 870
NmuCI GTSAC 2 cut(s) 70, 924
NsiI ATGCAT 1 cut(s) 941
PcsI WCGNNNNNNNCGW 1 cut(s) 504
PctI GAATGC 1 cut(s) 939
PdiI GCCGGC 1 cut(s) 502
PdmI GAANNNNTTC 2 cut(s) 834, 880
PfeI GAWTC 2 cut(s) 306, 796
PflFI GACNNNGTC 1 cut(s) 365
PinAI ACCGGT 1 cut(s) 324
PkrI GCNGC 4 cut(s) 253, 438, 692, 781
Psp6I CCWGG 1 cut(s) 871
PspEI GGTNACC 1 cut(s) 924
PspGI CCWGG 1 cut(s) 871
PspN4I GGNNCC 5 cut(s) 232, 386, 409, 454, 870
PspPI GGNCC 2 cut(s) 333, 542
PsyI GACNNNGTC 1 cut(s) 365
RsaI GTAC 2 cut(s) 329, 454
RsaNI GTAC 2 cut(s) 328, 453
RseI CAYNNNNRTG 2 cut(s) 149, 462
SaqAI TTAA 4 cut(s) 537, 647, 747, 851
SatI GCNGC 4 cut(s) 252, 437, 691, 780
Sau3AI GATC 3 cut(s) 99, 270, 484
Sau96I GGNCC 2 cut(s) 333, 542
ScrFI CCNGG 4 cut(s) 228, 471, 873, 912
SduI GDGCHC 2 cut(s) 233, 721
SfaNI GCATC 2 cut(s) 454, 926
SinI GGWCC 2 cut(s) 333, 542
SmiMI CAYNNNNRTG 2 cut(s) 149, 462
Sse9I AATT 2 cut(s) 23, 759
SsiI CCGC 3 cut(s) 106, 252, 585
SspMI CTAG 3 cut(s) 89, 554, 818
StyD4I CCNGG 4 cut(s) 226, 469, 871, 910
StyI CCWWGG 2 cut(s) 566, 615
TaaI ACNGT 2 cut(s) 57, 723
TaiI ACGT 4 cut(s) 462, 480, 564, 867
TaqI TCGA 7 cut(s) 243, 312, 462, 483, 495, 507, 513
TasI AATT 2 cut(s) 23, 759
TauI GCSGC 1 cut(s) 254
TfiI GAWTC 2 cut(s) 306, 796
Tru1I TTAA 4 cut(s) 537, 647, 747, 851
Tru9I TTAA 4 cut(s) 537, 647, 747, 851
TscAI CASTG 6 cut(s) 75, 139, 454, 728, 745, 896
TseFI GTSAC 2 cut(s) 70, 924
TseI GCWGC 3 cut(s) 436, 690, 779
Tsp45I GTSAC 2 cut(s) 70, 924
TspDTI ATGAA 4 cut(s) 167, 413, 446, 611
TspGWI ACGGA 3 cut(s) 187, 229, 763
TspRI CASTG 6 cut(s) 75, 139, 454, 728, 745, 896
Tth111I GACNNNGTC 1 cut(s) 365
VpaK11BI GGWCC 2 cut(s) 333, 542
XapI RAATTY 1 cut(s) 23
XbaI TCTAGA 1 cut(s) 817
XmnI GAANNNNTTC 2 cut(s) 834, 880
XspI CTAG 3 cut(s) 89, 554, 818
Zsp2I ATGCAT 1 cut(s) 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.