AT1G44542

cyclase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000645
Physical Location & Seq
Reverse (-)
16864831 .. 16866787
1957 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G44542.1

Sequence Viewer

Length: 816 bp
ATGTACCATCTCCTTATCATTATCACTACACTCTCCTTCTCTTCAATTAACATAACCTTCGCCGTCGATGAAGCTTTCCCTTCAATTCCCACTACCTTCAGCGTCGCAACGAAACAACACTACGACGTAAAACCAATCCACCATGAAGTTTATGACGGAGAAAGGAAGATATACGACATCAGCCACCAGTACACGCCGGAGTTGCCGGTTTGGGAGTCTTCAGAAGGACTAGGGAACTTTCTTAGACTTGCCGTGAGTATGAAGAATGGATCCGATGCTAATATCTCGAAGATGGAACTATCTGTTCACTCTGGAACTCATGTTGATGCACCAGGCCATTTCCATGACCATTATTATGAGTCTGGTTTTGATACTGATTCACTTGATCTTCAAATCCTTAATGGTCCTGCTTTATTGGTTGATGTTCCAAGAGATAAGAACATTTCAGCTGAGGTTATGAAATCACTACATATTCCAAGAGGGATCCGTCGTGTTCTCTTTAAAACATTGAACACTGATAGGAGGCTTATGTTTAAGAAAGAATTTGATTCAAGCTTTGTCGGGTTTATGGTCGATGGGGCGAAATGGTTGGTTGAAAATACAGACATCAAACTTGTTGGGCTTGATTATCTTTCATTTGCTGCTTATGATGAAGCACCTGCGACGCATAGGTTTATACTTGAACGACGGGATATAATCCCTGTCGAAGCGCTGAAGCTGGATGACGTGGAGGTAGGAATGTACACGCTTCATTGCTTACCGTTAAGATTGGTTGGAGCGGAAGGAGCACCAACGAGATGCATTCTCATCAAGTGA

Protein Analysis

271

Amino Acids

30.64

Weight (kDa)

5.68

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 58 - 213 2.8e-20 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 667
Acc36I ACCTGC 1 cut(s) 667
AccBSI CCGCTC 1 cut(s) 779
AciI CCGC 1 cut(s) 779
AclWI GGATC 4 cut(s) 264, 277, 478, 491
AcsI RAATTY 1 cut(s) 542
AcuI CTGAAG 3 cut(s) 82, 204, 734
AfaI GTAC 3 cut(s) 5, 191, 743
AfeI AGCGCT 1 cut(s) 711
AgsI TTSAA 7 cut(s) 45, 84, 392, 511, 552, 596, 683
AjiI CACGTC 1 cut(s) 727
AjnI CCWGG 1 cut(s) 331
AluBI AGCT 4 cut(s) 74, 449, 555, 718
AluI AGCT 4 cut(s) 74, 449, 555, 718
Alw21I GWGCWC 1 cut(s) 790
AlwI GGATC 4 cut(s) 264, 277, 478, 491
Aor51HI AGCGCT 1 cut(s) 711
AoxI GGCC 1 cut(s) 334
ApeKI GCWGC 1 cut(s) 641
ApoI RAATTY 1 cut(s) 542
AspLEI GCGC 1 cut(s) 712
AspS9I GGNCC 1 cut(s) 404
AvaII GGWCC 1 cut(s) 404
BamHI GGATCC 2 cut(s) 269, 483
BbsI GAAGAC 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 790
BbvCI CCTCAGC 1 cut(s) 450
BbvI GCAGC 1 cut(s) 628
BccI CCATC 3 cut(s) 15, 286, 569
BceAI ACGGC 2 cut(s) 47, 236
BciT130I CCWGG 1 cut(s) 333
BfaI CTAG 1 cut(s) 230
BfoI RGCGCY 1 cut(s) 713
BfuAI ACCTGC 1 cut(s) 667
BisI GCNGC 1 cut(s) 642
BlsI GCNGC 1 cut(s) 643
Bme1390I CCNGG 1 cut(s) 333
Bme18I GGWCC 1 cut(s) 404
BmgBI CACGTC 1 cut(s) 727
BmgT120I GGNCC 1 cut(s) 404
BmiI GGNNCC 2 cut(s) 271, 485
BmrFI CCNGG 1 cut(s) 333
BmsI GCATC 3 cut(s) 265, 316, 788
BpiI GAAGAC 1 cut(s) 210
Bpu10I CCTNAGC 1 cut(s) 450
Bse118I RCCGGY 1 cut(s) 205
Bse1I ACTGG 1 cut(s) 187
Bse3DI GCAATG 1 cut(s) 751
BseBI CCWGG 1 cut(s) 333
BseGI GGATG 1 cut(s) 727
BseMI GCAATG 1 cut(s) 751
BseMII CTCAG 1 cut(s) 441
BseNI ACTGG 1 cut(s) 187
BseXI GCAGC 1 cut(s) 628
BshFI GGCC 1 cut(s) 336
BsiHKAI GWGCWC 1 cut(s) 790
BsiSI CCGG 2 cut(s) 197, 206
BsmI GAATGC 1 cut(s) 801
BsnI GGCC 1 cut(s) 336
Bsp1286I GDGCHC 1 cut(s) 790
Bsp1407I TGTACA 1 cut(s) 741
Bsp143I GATC 3 cut(s) 269, 385, 483
BspACI CCGC 1 cut(s) 779
BspANI GGCC 1 cut(s) 336
BspCNI CTCAG 1 cut(s) 442
BspLI GGNNCC 2 cut(s) 271, 485
BspMI ACCTGC 1 cut(s) 667
BspPI GGATC 4 cut(s) 264, 277, 478, 491
BsrBI CCGCTC 1 cut(s) 779
BsrDI GCAATG 1 cut(s) 751
BsrFI RCCGGY 1 cut(s) 205
BsrGI TGTACA 1 cut(s) 741
BsrI ACTGG 1 cut(s) 187
BssAI RCCGGY 1 cut(s) 205
BssMI GATC 3 cut(s) 269, 385, 483
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 1 cut(s) 762
Bst6I CTCTTC 1 cut(s) 46
BstAUI TGTACA 1 cut(s) 741
BstDEI CTNAG 2 cut(s) 242, 450
BstF5I GGATG 1 cut(s) 727
BstH2I RGCGCY 1 cut(s) 713
BstHHI GCGC 1 cut(s) 712
BstKTI GATC 3 cut(s) 272, 388, 486
BstMBI GATC 3 cut(s) 269, 385, 483
BstMWI GCNNNNNNNGC 2 cut(s) 202, 785
BstNI CCWGG 1 cut(s) 333
BstSCI CCNGG 1 cut(s) 331
BstV1I GCAGC 1 cut(s) 628
BstV2I GAAGAC 1 cut(s) 210
BstX2I RGATCY 2 cut(s) 269, 483
BstYI RGATCY 2 cut(s) 269, 483
BsuRI GGCC 1 cut(s) 336
BtrI CACGTC 1 cut(s) 727
BtsCI GGATG 1 cut(s) 727
BtsIMutI CAGTG 1 cut(s) 513
BveI ACCTGC 1 cut(s) 667
CfoI GCGC 1 cut(s) 712
Cfr10I RCCGGY 1 cut(s) 205
Cfr13I GGNCC 1 cut(s) 404
CseI GACGC 2 cut(s) 91, 673
Csp6I GTAC 3 cut(s) 4, 190, 742
CviAII CATG 3 cut(s) 143, 320, 344
CviJI RGCY 8 cut(s) 74, 183, 336, 449, 526, 555, 622, 718
CviKI_1 RGCY 8 cut(s) 74, 183, 336, 449, 526, 555, 622, 718
CviQI GTAC 3 cut(s) 4, 190, 742
DdeI CTNAG 2 cut(s) 242, 450
DpnI GATC 3 cut(s) 271, 387, 485
DpnII GATC 3 cut(s) 269, 385, 483
DraI TTTAAA 1 cut(s) 502
Eam1104I CTCTTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 46
Eco47I GGWCC 1 cut(s) 404
Eco47III AGCGCT 1 cut(s) 711
Eco57I CTGAAG 3 cut(s) 82, 204, 734
EcoRII CCWGG 1 cut(s) 331
EcoT22I ATGCAT 1 cut(s) 803
FaeI CATG 3 cut(s) 146, 323, 347
FatI CATG 3 cut(s) 142, 319, 343
Fnu4HI GCNGC 1 cut(s) 642
FokI GGATG 1 cut(s) 734
Fsp4HI GCNGC 1 cut(s) 642
FspBI CTAG 1 cut(s) 230
GlaI GCGC 1 cut(s) 711
GluI GCNGC 1 cut(s) 642
HaeII RGCGCY 1 cut(s) 713
HaeIII GGCC 1 cut(s) 336
HapII CCGG 2 cut(s) 197, 206
HgaI GACGC 2 cut(s) 91, 673
HhaI GCGC 1 cut(s) 712
Hin1II CATG 3 cut(s) 146, 323, 347
Hin6I GCGC 1 cut(s) 710
HinP1I GCGC 1 cut(s) 710
HindIII AAGCTT 2 cut(s) 72, 553
HinfI GANTC 4 cut(s) 215, 359, 377, 548
HpaII CCGG 2 cut(s) 197, 206
Hpy166II GTNNAC 3 cut(s) 192, 307, 744
Hpy188I TCNGA 2 cut(s) 223, 274
Hpy188III TCNNGA 2 cut(s) 286, 312
Hpy8I GTNNAC 3 cut(s) 192, 307, 744
Hpy99I CGWCG 6 cut(s) 68, 107, 128, 492, 667, 690
HpyAV CCTTC 6 cut(s) 46, 67, 90, 106, 218, 776
HpyCH4III ACNGT 1 cut(s) 762
HpyCH4IV ACGT 2 cut(s) 126, 726
HpyCH4V TGCA 2 cut(s) 329, 801
HpyF10VI GCNNNNNNNGC 2 cut(s) 202, 785
HpyF3I CTNAG 2 cut(s) 242, 450
HpySE526I ACGT 2 cut(s) 126, 726
Hsp92II CATG 3 cut(s) 146, 323, 347
HspAI GCGC 1 cut(s) 710
Kzo9I GATC 3 cut(s) 269, 385, 483
LmnI GCTCC 2 cut(s) 776, 785
Lsp1109I GCAGC 1 cut(s) 628
LweI GCATC 3 cut(s) 265, 316, 788
MaeI CTAG 1 cut(s) 230
MaeII ACGT 2 cut(s) 126, 726
MalI GATC 3 cut(s) 271, 387, 485
MbiI CCGCTC 1 cut(s) 779
MboI GATC 3 cut(s) 269, 385, 483
MboII GAAGA 6 cut(s) 33, 178, 210, 274, 301, 380
MflI RGATCY 2 cut(s) 269, 483
MhlI GDGCHC 1 cut(s) 790
MluCI AATT 3 cut(s) 45, 84, 542
MlyI GAGTC 2 cut(s) 224, 368
MmeI TCCRAC 1 cut(s) 754
MnlI CCTC 4 cut(s) 445, 473, 516, 724
Mph1103I ATGCAT 1 cut(s) 803
MseI TTAA 5 cut(s) 48, 399, 501, 534, 764
MslI CAYNNNNRTG 3 cut(s) 324, 342, 354
MspA1I CMGCKG 1 cut(s) 449
MspI CCGG 2 cut(s) 197, 206
MspR9I CCNGG 1 cut(s) 333
Mva1269I GAATGC 1 cut(s) 801
MvaI CCWGG 1 cut(s) 333
MwoI GCNNNNNNNGC 2 cut(s) 202, 785
NdeII GATC 3 cut(s) 269, 385, 483
NlaIII CATG 3 cut(s) 146, 323, 347
NlaIV GGNNCC 2 cut(s) 271, 485
NsiI ATGCAT 1 cut(s) 803
PaqCI CACCTGC 1 cut(s) 667
PctI GAATGC 1 cut(s) 801
PfeI GAWTC 2 cut(s) 377, 548
PkrI GCNGC 1 cut(s) 643
PleI GAGTC 2 cut(s) 223, 367
PpsI GAGTC 2 cut(s) 223, 367
Psp6I CCWGG 1 cut(s) 331
PspGI CCWGG 1 cut(s) 331
PspN4I GGNNCC 2 cut(s) 271, 485
PspPI GGNCC 1 cut(s) 404
PsuI RGATCY 2 cut(s) 269, 483
PvuII CAGCTG 1 cut(s) 449
RsaI GTAC 3 cut(s) 5, 191, 743
RsaNI GTAC 3 cut(s) 4, 190, 742
RseI CAYNNNNRTG 3 cut(s) 324, 342, 354
SaqAI TTAA 5 cut(s) 48, 399, 501, 534, 764
SatI GCNGC 1 cut(s) 642
Sau3AI GATC 3 cut(s) 269, 385, 483
Sau96I GGNCC 1 cut(s) 404
SchI GAGTC 2 cut(s) 224, 368
ScrFI CCNGG 1 cut(s) 333
SduI GDGCHC 1 cut(s) 790
SfaNI GCATC 3 cut(s) 265, 316, 788
SinI GGWCC 1 cut(s) 404
SmiMI CAYNNNNRTG 3 cut(s) 324, 342, 354
Sse9I AATT 3 cut(s) 45, 84, 542
SsiI CCGC 1 cut(s) 779
SspMI CTAG 1 cut(s) 230
StyD4I CCNGG 1 cut(s) 331
TaaI ACNGT 1 cut(s) 762
TaiI ACGT 2 cut(s) 129, 729
TaqI TCGA 4 cut(s) 66, 287, 573, 705
TasI AATT 3 cut(s) 45, 84, 542
TatI WGTACW 2 cut(s) 189, 741
TfiI GAWTC 2 cut(s) 377, 548
Tru1I TTAA 5 cut(s) 48, 399, 501, 534, 764
Tru9I TTAA 5 cut(s) 48, 399, 501, 534, 764
TscAI CASTG 1 cut(s) 520
TseI GCWGC 1 cut(s) 641
TspDTI ATGAA 7 cut(s) 84, 159, 275, 473, 624, 666, 740
TspGWI ACGGA 2 cut(s) 171, 476
TspRI CASTG 1 cut(s) 520
VpaK11BI GGWCC 1 cut(s) 404
XapI RAATTY 1 cut(s) 542
XspI CTAG 1 cut(s) 230
Zsp2I ATGCAT 1 cut(s) 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.