Rroxscaffold_7G00209750

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000645
Physical Location & Seq
Reverse (-)
60009078 .. 60012403
3326 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00209750.1

Sequence Viewer

Length: 615 bp
ATGAACCGACCCCTTCTCCATCCCCTCTTTCTCACATTTTCTTGCTTATTCTTCCTTGCTTCCTCCTCCTCCTCCTCAGGGTACGGTCCCGGCGACACTCCAAAACCCGTTAGGAGGGAAGTATACGGCAATGGTAGAATCTTTGACATCACCCACAGGTACACCCCGGAGATTCCCGTGTATGGTTCAAAGCTTGGGATCGGACATTTCCGGTGGCTCCAACTGAGCATGAAGAATGGTTCACTTACCAACAACTCGGAATTCAAGCTGCCTGTTCACACCGGAACCCATGTTGATGCCCCTGGCCATGTGTTTGATGATTACTATGATGCTGGGTTTGATGTAGATCAGCTTGACTTGGAGGTACTCAATGGCCCTGCCCTTGTTGTTGATGTTCCCAGGGATAGCAACATAACTGGAACTGATTACTTTTCCATTGCTACCTATGATGAAGCTATTCCGGCACATATTGCTTTTCTAAAGGGAAGGGAAATCATACCTGTGGAAGGCCTAAAGCTTGATGGCATAAAACCAGGAAATTACTCTGTGCATTGCTTACCTCTAAGGTTGCTTGGTGCTGAAGGAGCACCAACAAGATGCATTCTCATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

204

Amino Acids

22.46

Weight (kDa)

6.03

Isoelectric Point (pI)

33.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 48 - 135 1.2e-12 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 123
AclWI GGATC 1 cut(s) 206
AcoI YGGCCR 1 cut(s) 304
AcsI RAATTY 1 cut(s) 260
AcuI CTGAAG 1 cut(s) 600
AfaI GTAC 3 cut(s) 83, 161, 366
AfiI CCNNNNNNNGG 4 cut(s) 78, 114, 182, 506
AgsI TTSAA 2 cut(s) 189, 265
AjnI CCWGG 3 cut(s) 301, 398, 532
AluBI AGCT 5 cut(s) 193, 268, 352, 455, 517
AluI AGCT 5 cut(s) 193, 268, 352, 455, 517
Alw21I GWGCWC 1 cut(s) 589
AlwI GGATC 1 cut(s) 206
AoxI GGCC 3 cut(s) 304, 373, 508
ApeKI GCWGC 1 cut(s) 268
ApoI RAATTY 1 cut(s) 260
Asp700I GAANNNNTTC 1 cut(s) 456
AspS9I GGNCC 2 cut(s) 86, 374
AsuC2I CCSGG 2 cut(s) 90, 167
AsuHPI GGTGA 1 cut(s) 142
AvaII GGWCC 1 cut(s) 86
AxyI CCTNAGG 1 cut(s) 76
BalI TGGCCA 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 589
BbvI GCAGC 1 cut(s) 255
BccI CCATC 2 cut(s) 27, 515
BceAI ACGGC 1 cut(s) 142
BciT130I CCWGG 3 cut(s) 303, 400, 534
BcnI CCSGG 2 cut(s) 90, 167
BisI GCNGC 1 cut(s) 269
BlsI GCNGC 1 cut(s) 270
Bme1390I CCNGG 5 cut(s) 90, 167, 303, 400, 534
Bme18I GGWCC 1 cut(s) 86
BmgT120I GGNCC 2 cut(s) 86, 374
BmiI GGNNCC 3 cut(s) 88, 218, 286
BmrFI CCNGG 5 cut(s) 90, 167, 303, 400, 534
BmsI GCATC 3 cut(s) 286, 319, 587
BpuMI CCSGG 2 cut(s) 90, 167
BsaBI GATNNNNATC 1 cut(s) 345
BsaJI CCNNGG 4 cut(s) 165, 301, 398, 399
BsaWI WCCGGW 2 cut(s) 210, 281
BsaXI ACNNNNNCTCC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 4 cut(s) 78, 114, 182, 506
Bse1I ACTGG 1 cut(s) 421
Bse21I CCTNAGG 1 cut(s) 76
Bse3DI GCAATG 3 cut(s) 136, 435, 550
Bse8I GATNNNNATC 1 cut(s) 345
BseBI CCWGG 3 cut(s) 303, 400, 534
BseDI CCNNGG 4 cut(s) 165, 301, 398, 399
BseGI GGATG 1 cut(s) 19
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 4 cut(s) 78, 114, 182, 506
BseMI GCAATG 3 cut(s) 136, 435, 550
BseMII CTCAG 2 cut(s) 90, 215
BseNI ACTGG 1 cut(s) 421
BseRI GAGGAG 4 cut(s) 55, 58, 61, 64
BseXI GCAGC 1 cut(s) 255
BseYI CCCAGC 1 cut(s) 332
BshFI GGCC 3 cut(s) 306, 375, 510
BsiHKAI GWGCWC 1 cut(s) 589
BsiSI CCGG 5 cut(s) 90, 167, 211, 282, 461
BslFI GGGAC 1 cut(s) 72
BslI CCNNNNNNNGG 4 cut(s) 78, 114, 182, 506
BsmFI GGGAC 1 cut(s) 72
BsmI GAATGC 1 cut(s) 600
BsnI GGCC 3 cut(s) 306, 375, 510
Bsp1286I GDGCHC 1 cut(s) 589
Bsp143I GATC 2 cut(s) 198, 346
BspANI GGCC 3 cut(s) 306, 375, 510
BspCNI CTCAG 2 cut(s) 89, 216
BspLI GGNNCC 3 cut(s) 88, 218, 286
BspPI GGATC 1 cut(s) 206
BsrDI GCAATG 3 cut(s) 136, 435, 550
BsrI ACTGG 1 cut(s) 421
BssECI CCNNGG 4 cut(s) 165, 301, 398, 399
BssMI GATC 2 cut(s) 198, 346
BssNAI GTATAC 1 cut(s) 124
Bst1107I GTATAC 1 cut(s) 124
Bst2UI CCWGG 3 cut(s) 303, 400, 534
Bst4CI ACNGT 1 cut(s) 86
BstAPI GCANNNNNTGC 1 cut(s) 470
BstDEI CTNAG 3 cut(s) 76, 224, 563
BstENI CCTNNNNNAGG 1 cut(s) 504
BstF5I GGATG 1 cut(s) 19
BstKTI GATC 2 cut(s) 201, 349
BstMBI GATC 2 cut(s) 198, 346
BstMWI GCNNNNNNNGC 3 cut(s) 461, 470, 584
BstNI CCWGG 3 cut(s) 303, 400, 534
BstSCI CCNGG 5 cut(s) 88, 165, 301, 398, 532
BstV1I GCAGC 1 cut(s) 255
BstZ17I GTATAC 1 cut(s) 124
Bsu36I CCTNAGG 1 cut(s) 76
BsuRI GGCC 3 cut(s) 306, 375, 510
BtsCI GGATG 1 cut(s) 19
Cfr13I GGNCC 2 cut(s) 86, 374
Csp6I GTAC 3 cut(s) 82, 160, 365
CviAII CATG 3 cut(s) 229, 290, 308
CviJI RGCY 9 cut(s) 193, 217, 268, 306, 352, 375, 455, 510, 517
CviKI_1 RGCY 9 cut(s) 193, 217, 268, 306, 352, 375, 455, 510, 517
CviQI GTAC 3 cut(s) 82, 160, 365
DdeI CTNAG 3 cut(s) 76, 224, 563
DpnI GATC 2 cut(s) 200, 348
DpnII GATC 2 cut(s) 198, 346
EaeI YGGCCR 1 cut(s) 304
Eco147I AGGCCT 1 cut(s) 510
Eco47I GGWCC 1 cut(s) 86
Eco57I CTGAAG 1 cut(s) 600
Eco81I CCTNAGG 1 cut(s) 76
EcoNI CCTNNNNNAGG 1 cut(s) 504
EcoRI GAATTC 1 cut(s) 260
EcoRII CCWGG 3 cut(s) 301, 398, 532
EcoT22I ATGCAT 1 cut(s) 602
FaeI CATG 3 cut(s) 232, 293, 311
FaqI GGGAC 1 cut(s) 72
FatI CATG 3 cut(s) 228, 289, 307
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 1 cut(s) 269
FokI GGATG 1 cut(s) 6
Fsp4HI GCNGC 1 cut(s) 269
GluI GCNGC 1 cut(s) 269
GsaI CCCAGC 1 cut(s) 336
HaeIII GGCC 3 cut(s) 306, 375, 510
HapII CCGG 5 cut(s) 90, 167, 211, 282, 461
Hin1II CATG 3 cut(s) 232, 293, 311
HindIII AAGCTT 2 cut(s) 191, 515
HinfI GANTC 2 cut(s) 138, 172
HpaII CCGG 5 cut(s) 90, 167, 211, 282, 461
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 4 cut(s) 124, 162, 242, 277
Hpy188I TCNGA 2 cut(s) 203, 259
Hpy8I GTNNAC 4 cut(s) 124, 162, 242, 277
HpyAV CCTTC 4 cut(s) 23, 480, 500, 575
HpyCH4III ACNGT 1 cut(s) 86
HpyCH4V TGCA 2 cut(s) 550, 600
HpyF10VI GCNNNNNNNGC 3 cut(s) 461, 470, 584
HpyF3I CTNAG 3 cut(s) 76, 224, 563
Hsp92II CATG 3 cut(s) 232, 293, 311
Kzo9I GATC 2 cut(s) 198, 346
LmnI GCTCC 2 cut(s) 222, 584
Lsp1109I GCAGC 1 cut(s) 255
LweI GCATC 3 cut(s) 286, 319, 587
MalI GATC 2 cut(s) 200, 348
MboI GATC 2 cut(s) 198, 346
MboII GAAGA 2 cut(s) 43, 244
MhlI GDGCHC 1 cut(s) 589
MlsI TGGCCA 1 cut(s) 306
MluCI AATT 2 cut(s) 260, 538
MluNI TGGCCA 1 cut(s) 306
MmeI TCCRAC 1 cut(s) 244
MnlI CCTC 9 cut(s) 35, 73, 76, 79, 82, 85, 108, 355, 570
Mox20I TGGCCA 1 cut(s) 306
Mph1103I ATGCAT 1 cut(s) 602
MroXI GAANNNNTTC 1 cut(s) 456
MscI TGGCCA 1 cut(s) 306
MslI CAYNNNNRTG 2 cut(s) 294, 500
Msp20I TGGCCA 1 cut(s) 306
MspI CCGG 5 cut(s) 90, 167, 211, 282, 461
MspR9I CCNGG 5 cut(s) 90, 167, 303, 400, 534
Mva1269I GAATGC 1 cut(s) 600
MvaI CCWGG 3 cut(s) 303, 400, 534
MwoI GCNNNNNNNGC 3 cut(s) 461, 470, 584
NciI CCSGG 2 cut(s) 90, 167
NdeII GATC 2 cut(s) 198, 346
NlaIII CATG 3 cut(s) 232, 293, 311
NlaIV GGNNCC 3 cut(s) 88, 218, 286
NsiI ATGCAT 1 cut(s) 602
PasI CCCWGGG 1 cut(s) 399
PceI AGGCCT 1 cut(s) 510
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 1 cut(s) 600
PdmI GAANNNNTTC 1 cut(s) 456
PfeI GAWTC 2 cut(s) 138, 172
PkrI GCNGC 1 cut(s) 270
Psp6I CCWGG 3 cut(s) 301, 398, 532
PspFI CCCAGC 1 cut(s) 332
PspGI CCWGG 3 cut(s) 301, 398, 532
PspN4I GGNNCC 3 cut(s) 88, 218, 286
PspPI GGNCC 2 cut(s) 86, 374
RsaI GTAC 3 cut(s) 83, 161, 366
RsaNI GTAC 3 cut(s) 82, 160, 365
RseI CAYNNNNRTG 2 cut(s) 294, 500
SatI GCNGC 1 cut(s) 269
Sau3AI GATC 2 cut(s) 198, 346
Sau96I GGNCC 2 cut(s) 86, 374
ScrFI CCNGG 5 cut(s) 90, 167, 303, 400, 534
SduI GDGCHC 1 cut(s) 589
SfaNI GCATC 3 cut(s) 286, 319, 587
SinI GGWCC 1 cut(s) 86
SmiMI CAYNNNNRTG 2 cut(s) 294, 500
Sse9I AATT 2 cut(s) 260, 538
SseBI AGGCCT 1 cut(s) 510
StuI AGGCCT 1 cut(s) 510
StyD4I CCNGG 5 cut(s) 88, 165, 301, 398, 532
TaaI ACNGT 1 cut(s) 86
TasI AATT 2 cut(s) 260, 538
TfiI GAWTC 2 cut(s) 138, 172
TseI GCWGC 1 cut(s) 268
TspDTI ATGAA 3 cut(s) 17, 245, 465
VpaK11BI GGWCC 1 cut(s) 86
XagI CCTNNNNNAGG 1 cut(s) 504
XapI RAATTY 1 cut(s) 260
XmiI GTMKAC 1 cut(s) 123
XmnI GAANNNNTTC 1 cut(s) 456
Zsp2I ATGCAT 1 cut(s) 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.