Rroxscaffold_7G00209790

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000645
Physical Location & Seq
Reverse (-)
60055720 .. 60059442
3723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00209790.1

Sequence Viewer

Length: 819 bp
ATGAATCCAATTCTCCTCCTCCTCCTCCTCTTCTGCTCCGTTCCAGCCACCCTCTCCCACGCCACCACAGCCTACCCTTCCATTCCGGGCACCACCCCCGTTGACGTCTCCTCCGGCGCCACCGCCACCAACCTGATCCCCCCTCGCCGCGAAGTCCACGGAAACGGCCGAATCTTTGACATCAGCCACCGCTACAGCCCCGACATGCCGGCGTTTGGCTCCGTCGACGGCGTCGGCCAGTTCCTGTGGCTCCCCAACAGCATGAAGAACGGCTCCATTGCTAACAACTCCGAGATGAAGCTCCCCACCCACACCGGGACCCACGTCGACGCCCCCGGCCACGTCTTCGATCACTACTTCGACGCCGGCTTCGACGTCGACACGCTCGACTTGGATGTCCTTAATGGTGCGGCATTGTTAGTTGACGTTCCGAGAGATAAGAACATTACTGCTGAAGTCATGGAGGGCTTACATATTCCCAAGGGTGTGCGTCGTGTACTTTTCAGAACTTTGAACACAGACAGACGGCTTATGTTTCAAAAGGAGTTTGACAGCAGCTATGTGGGATTTATGAGGGATGGAGCTAAGTGGTTGGTGGAGAACACCGACATCAAACTGATTGGAATTGATTACTTATCTGCTGCTGCATATGATGATTTGATTCCGTCTCACCTTGTTTTGCTAGAAGGCAGGGAGATCATTCTTGTAGAAGGTCTAAAACTTGATGACATCCAACCAGGAATATATTCTGTCCATTGCTTACCTTTGAGGCTGACAGGTGCAGAAGGATCACCAATCAGATGCATTCTTATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

272

Amino Acids

29.84

Weight (kDa)

5.51

Isoelectric Point (pI)

31.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 59 - 214 2.8e-21 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 395
AatII GACGTC 2 cut(s) 108, 378
AccB1I GGYRCC 2 cut(s) 89, 116
AccI GTMKAC 3 cut(s) 225, 327, 378
AccII CGCG 1 cut(s) 150
AciI CCGC 4 cut(s) 123, 148, 190, 410
AclWI GGATC 2 cut(s) 130, 796
AcoI YGGCCR 3 cut(s) 166, 235, 337
AcuI CTGAAG 1 cut(s) 474
AcyI GRCGYC 6 cut(s) 105, 117, 231, 330, 363, 375
AfaI GTAC 1 cut(s) 498
AfiI CCNNNNNNNGG 2 cut(s) 215, 315
AgsI TTSAA 2 cut(s) 514, 539
AjiI CACGTC 2 cut(s) 325, 343
AjnI CCWGG 1 cut(s) 736
AluBI AGCT 3 cut(s) 301, 558, 584
AluI AGCT 3 cut(s) 301, 558, 584
Alw26I GTCTC 2 cut(s) 112, 672
AlwI GGATC 2 cut(s) 130, 796
AlwNI CAGNNNCTG 1 cut(s) 244
AoxI GGCC 3 cut(s) 166, 235, 337
ApeKI GCWGC 3 cut(s) 555, 641, 644
ArsI GACNNNNNNTTYG 2 cut(s) 353, 385
Asp700I GAANNNNTTC 1 cut(s) 745
AspLEI GCGC 1 cut(s) 119
AspS9I GGNCC 1 cut(s) 318
AsuC2I CCSGG 3 cut(s) 87, 316, 336
AsuHPI GGTGA 2 cut(s) 662, 783
AvaII GGWCC 1 cut(s) 318
BaeGI GKGCMC 1 cut(s) 92
BanI GGYRCC 2 cut(s) 89, 116
BbsI GAAGAC 1 cut(s) 337
BbvI GCAGC 3 cut(s) 567, 628, 631
BccI CCATC 1 cut(s) 572
BceAI ACGGC 4 cut(s) 181, 244, 286, 542
BciT130I CCWGG 1 cut(s) 738
BcnI CCSGG 3 cut(s) 87, 316, 336
BcoDI GTCTC 2 cut(s) 112, 672
BfaI CTAG 1 cut(s) 683
BfmI CTRYAG 1 cut(s) 193
BfoI RGCGCY 1 cut(s) 120
BisI GCNGC 5 cut(s) 148, 411, 556, 642, 645
BlsI GCNGC 5 cut(s) 149, 412, 557, 643, 646
Bme1390I CCNGG 4 cut(s) 87, 316, 336, 738
Bme18I GGWCC 1 cut(s) 318
BmgBI CACGTC 2 cut(s) 325, 343
BmgT120I GGNCC 1 cut(s) 318
BmiI GGNNCC 7 cut(s) 91, 118, 220, 251, 274, 319, 320
BmrFI CCNGG 4 cut(s) 87, 316, 336, 738
BmsI GCATC 1 cut(s) 791
BoxI GACNNNNGTC 1 cut(s) 323
BpiI GAAGAC 1 cut(s) 337
BpuMI CCSGG 3 cut(s) 87, 316, 336
BsaHI GRCGYC 6 cut(s) 105, 117, 231, 330, 363, 375
BsaJI CCNNGG 3 cut(s) 157, 334, 480
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 315
Bse118I RCCGGY 2 cut(s) 208, 365
Bse1I ACTGG 1 cut(s) 238
Bse3DI GCAATG 2 cut(s) 276, 754
BseBI CCWGG 1 cut(s) 738
BseDI CCNNGG 3 cut(s) 157, 334, 480
BseGI GGATG 3 cut(s) 400, 583, 729
BseLI CCNNNNNNNGG 2 cut(s) 215, 315
BseMI GCAATG 2 cut(s) 276, 754
BseNI ACTGG 1 cut(s) 238
BseRI GAGGAG 6 cut(s) 5, 8, 11, 14, 17, 100
BseSI GKGCMC 1 cut(s) 92
BseX3I CGGCCG 1 cut(s) 166
BseXI GCAGC 3 cut(s) 567, 628, 631
BsgI GTGCAG 1 cut(s) 801
Bsh1236I CGCG 1 cut(s) 150
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 3 cut(s) 168, 237, 339
BshNI GGYRCC 2 cut(s) 89, 116
BsiEI CGRYCG 1 cut(s) 169
BsiSI CCGG 6 cut(s) 86, 114, 209, 315, 336, 366
BslFI GGGAC 1 cut(s) 331
BslI CCNNNNNNNGG 2 cut(s) 215, 315
BsmAI GTCTC 2 cut(s) 112, 672
BsmBI CGTCTC 2 cut(s) 112, 672
BsmFI GGGAC 1 cut(s) 331
BsmI GAATGC 1 cut(s) 804
BsnI GGCC 3 cut(s) 168, 237, 339
Bsp1286I GDGCHC 1 cut(s) 92
Bsp143I GATC 4 cut(s) 135, 349, 696, 788
BspACI CCGC 4 cut(s) 123, 148, 190, 410
BspANI GGCC 3 cut(s) 168, 237, 339
BspFNI CGCG 1 cut(s) 150
BspLI GGNNCC 7 cut(s) 91, 118, 220, 251, 274, 319, 320
BspPI GGATC 2 cut(s) 130, 796
BspT107I GGYRCC 2 cut(s) 89, 116
BsrDI GCAATG 2 cut(s) 276, 754
BsrFI RCCGGY 2 cut(s) 208, 365
BsrI ACTGG 1 cut(s) 238
BssAI RCCGGY 2 cut(s) 208, 365
BssECI CCNNGG 3 cut(s) 157, 334, 480
BssMI GATC 4 cut(s) 135, 349, 696, 788
BssNI GRCGYC 6 cut(s) 105, 117, 231, 330, 363, 375
BssT1I CCWWGG 1 cut(s) 480
Bst2UI CCWGG 1 cut(s) 738
Bst6I CTCTTC 1 cut(s) 35
BstACI GRCGYC 6 cut(s) 105, 117, 231, 330, 363, 375
BstC8I GCNNGC 2 cut(s) 210, 367
BstDEI CTNAG 1 cut(s) 585
BstDSI CCRYGG 1 cut(s) 157
BstF5I GGATG 3 cut(s) 400, 583, 729
BstFNI CGCG 1 cut(s) 150
BstH2I RGCGCY 1 cut(s) 120
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 4 cut(s) 138, 352, 699, 791
BstMAI GTCTC 2 cut(s) 112, 672
BstMBI GATC 4 cut(s) 135, 349, 696, 788
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNI CCWGG 1 cut(s) 738
BstNSI RCATGY 1 cut(s) 208
BstPAI GACNNNNGTC 1 cut(s) 323
BstSCI CCNGG 4 cut(s) 85, 314, 334, 736
BstSFI CTRYAG 1 cut(s) 193
BstSLI GKGCMC 1 cut(s) 92
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 3 cut(s) 567, 628, 631
BstV2I GAAGAC 1 cut(s) 337
BstZI CGGCCG 1 cut(s) 166
BsuRI GGCC 3 cut(s) 168, 237, 339
BtgI CCRYGG 1 cut(s) 157
BtrI CACGTC 2 cut(s) 325, 343
BtsCI GGATG 3 cut(s) 400, 583, 729
Cac8I GCNNGC 2 cut(s) 210, 367
CaiI CAGNNNCTG 1 cut(s) 244
CfoI GCGC 1 cut(s) 119
Cfr10I RCCGGY 2 cut(s) 208, 365
Cfr13I GGNCC 1 cut(s) 318
CseI GACGC 4 cut(s) 220, 338, 371, 479
Csp6I GTAC 1 cut(s) 497
CspCI CAANNNNNGTGG 2 cut(s) 82, 117
CviAII CATG 3 cut(s) 205, 262, 460
CviQI GTAC 1 cut(s) 497
DdeI CTNAG 1 cut(s) 585
DinI GGCGCC 1 cut(s) 118
DpnI GATC 4 cut(s) 137, 351, 698, 790
DpnII GATC 4 cut(s) 135, 349, 696, 788
DrdI GACNNNNNNGTC 1 cut(s) 395
DseDI GACNNNNNNGTC 1 cut(s) 395
EaeI YGGCCR 3 cut(s) 166, 235, 337
EagI CGGCCG 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
EclXI CGGCCG 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 480
Eco47I GGWCC 1 cut(s) 318
Eco52I CGGCCG 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 474
EcoO109I RGGNCCY 1 cut(s) 318
EcoRII CCWGG 1 cut(s) 736
EcoT14I CCWWGG 1 cut(s) 480
EcoT22I ATGCAT 1 cut(s) 806
EgeI GGCGCC 1 cut(s) 118
EheI GGCGCC 1 cut(s) 118
ErhI CCWWGG 1 cut(s) 480
Esp3I CGTCTC 2 cut(s) 112, 672
FaeI CATG 3 cut(s) 208, 265, 463
FaqI GGGAC 1 cut(s) 331
FatI CATG 3 cut(s) 204, 261, 459
FauNDI CATATG 1 cut(s) 649
FblI GTMKAC 3 cut(s) 225, 327, 378
Fnu4HI GCNGC 5 cut(s) 148, 411, 556, 642, 645
FokI GGATG 3 cut(s) 407, 590, 716
Fsp4HI GCNGC 5 cut(s) 148, 411, 556, 642, 645
FspBI CTAG 1 cut(s) 683
GlaI GCGC 1 cut(s) 118
GluI GCNGC 5 cut(s) 148, 411, 556, 642, 645
HaeII RGCGCY 1 cut(s) 120
HaeIII GGCC 3 cut(s) 168, 237, 339
HapII CCGG 6 cut(s) 86, 114, 209, 315, 336, 366
HgaI GACGC 4 cut(s) 220, 338, 371, 479
HhaI GCGC 1 cut(s) 119
Hin1I GRCGYC 6 cut(s) 105, 117, 231, 330, 363, 375
Hin1II CATG 3 cut(s) 208, 265, 463
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HincII GTYRAC 5 cut(s) 103, 226, 328, 379, 424
HindII GTYRAC 5 cut(s) 103, 226, 328, 379, 424
HinfI GANTC 3 cut(s) 4, 171, 661
HpaII CCGG 6 cut(s) 86, 114, 209, 315, 336, 366
HphI GGTGA 2 cut(s) 662, 783
Hpy166II GTNNAC 7 cut(s) 103, 157, 226, 328, 379, 424, 497
Hpy188I TCNGA 4 cut(s) 292, 432, 506, 800
Hpy8I GTNNAC 7 cut(s) 103, 157, 226, 328, 379, 424, 497
Hpy99I CGWCG 9 cut(s) 227, 230, 236, 329, 332, 365, 377, 380, 495
HpyAV CCTTC 4 cut(s) 87, 680, 704, 779
HpyCH4IV ACGT 5 cut(s) 105, 324, 342, 375, 426
HpyCH4V TGCA 3 cut(s) 647, 782, 804
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 1 cut(s) 585
HpySE526I ACGT 5 cut(s) 105, 324, 342, 375, 426
Hsp92I GRCGYC 6 cut(s) 105, 117, 231, 330, 363, 375
Hsp92II CATG 3 cut(s) 208, 265, 463
HspAI GCGC 1 cut(s) 117
KasI GGCGCC 1 cut(s) 116
KflI GGGWCCC 1 cut(s) 318
KroI GCCGGC 2 cut(s) 208, 365
KroNI GCCGGC 2 cut(s) 210, 367
Kzo9I GATC 4 cut(s) 135, 349, 696, 788
LmnI GCTCC 6 cut(s) 41, 224, 255, 278, 306, 581
Lsp1109I GCAGC 3 cut(s) 567, 628, 631
LweI GCATC 1 cut(s) 791
MaeI CTAG 1 cut(s) 683
MaeII ACGT 5 cut(s) 105, 324, 342, 375, 426
MalI GATC 4 cut(s) 137, 351, 698, 790
MboI GATC 4 cut(s) 135, 349, 696, 788
MboII GAAGA 3 cut(s) 22, 277, 337
MhlI GDGCHC 1 cut(s) 92
MluCI AATT 2 cut(s) 9, 624
Mly113I GGCGCC 1 cut(s) 117
MmeI TCCRAC 1 cut(s) 757
Mph1103I ATGCAT 1 cut(s) 806
MroNI GCCGGC 2 cut(s) 208, 365
MroXI GAANNNNTTC 1 cut(s) 745
MseI TTAA 1 cut(s) 402
MspI CCGG 6 cut(s) 86, 114, 209, 315, 336, 366
MspR9I CCNGG 4 cut(s) 87, 316, 336, 738
Mva1269I GAATGC 1 cut(s) 804
MvaI CCWGG 1 cut(s) 738
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 1 cut(s) 68
NaeI GCCGGC 2 cut(s) 210, 367
NarI GGCGCC 1 cut(s) 117
NciI CCSGG 3 cut(s) 87, 316, 336
NdeI CATATG 1 cut(s) 649
NdeII GATC 4 cut(s) 135, 349, 696, 788
NgoMIV GCCGGC 2 cut(s) 208, 365
NlaIII CATG 3 cut(s) 208, 265, 463
NlaIV GGNNCC 7 cut(s) 91, 118, 220, 251, 274, 319, 320
NsiI ATGCAT 1 cut(s) 806
NspI RCATGY 1 cut(s) 208
PcsI WCGNNNNNNNCGW 2 cut(s) 369, 384
PctI GAATGC 1 cut(s) 804
PdiI GCCGGC 2 cut(s) 210, 367
PdmI GAANNNNTTC 1 cut(s) 745
PfeI GAWTC 3 cut(s) 4, 171, 661
PflFI GACNNNGTC 1 cut(s) 230
PkrI GCNGC 5 cut(s) 149, 412, 557, 643, 646
PluTI GGCGCC 1 cut(s) 120
PpuMI RGGWCCY 1 cut(s) 318
PshAI GACNNNNGTC 1 cut(s) 323
Psp5II RGGWCCY 1 cut(s) 318
Psp6I CCWGG 1 cut(s) 736
PspGI CCWGG 1 cut(s) 736
PspN4I GGNNCC 7 cut(s) 91, 118, 220, 251, 274, 319, 320
PspPI GGNCC 1 cut(s) 318
PspPPI RGGWCCY 1 cut(s) 318
PstNI CAGNNNCTG 1 cut(s) 244
PsyI GACNNNGTC 1 cut(s) 230
RsaI GTAC 1 cut(s) 498
RsaNI GTAC 1 cut(s) 497
SalI GTCGAC 3 cut(s) 224, 326, 377
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 5 cut(s) 148, 411, 556, 642, 645
Sau3AI GATC 4 cut(s) 135, 349, 696, 788
Sau96I GGNCC 1 cut(s) 318
ScrFI CCNGG 4 cut(s) 87, 316, 336, 738
SduI GDGCHC 1 cut(s) 92
SfaNI GCATC 1 cut(s) 791
SfcI CTRYAG 1 cut(s) 193
SfoI GGCGCC 1 cut(s) 118
SgrDI CGTCGACG 2 cut(s) 224, 326
SinI GGWCC 1 cut(s) 318
Sse9I AATT 2 cut(s) 9, 624
SsiI CCGC 4 cut(s) 123, 148, 190, 410
SspDI GGCGCC 1 cut(s) 116
SspMI CTAG 1 cut(s) 683
StyD4I CCNGG 4 cut(s) 85, 314, 334, 736
StyI CCWWGG 1 cut(s) 480
TaiI ACGT 5 cut(s) 108, 327, 345, 378, 429
TaqI TCGA 7 cut(s) 225, 327, 348, 360, 372, 378, 387
TasI AATT 2 cut(s) 9, 624
TatI WGTACW 1 cut(s) 496
TauI GCSGC 2 cut(s) 150, 413
TfiI GAWTC 3 cut(s) 4, 171, 661
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TseI GCWGC 3 cut(s) 555, 641, 644
TspDTI ATGAA 3 cut(s) 17, 278, 311
TspGWI ACGGA 4 cut(s) 28, 174, 211, 654
Tth111I GACNNNGTC 1 cut(s) 230
VpaK11BI GGWCC 1 cut(s) 318
XceI RCATGY 1 cut(s) 208
XmiI GTMKAC 3 cut(s) 225, 327, 378
XmnI GAANNNNTTC 1 cut(s) 745
XspI CTAG 1 cut(s) 683
ZraI GACGTC 2 cut(s) 106, 376
Zsp2I ATGCAT 1 cut(s) 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.