Rmu_sc0010421.1_g000001

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010421.1
OG0000645
Physical Location & Seq
Reverse (-)
101 .. 4608
4508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010421.1_g000001.1.cds

Sequence Viewer

Length: 765 bp
atgaagaccttattctttctcctcctactctgctccctttcgctcacactccccactctctcccacgccaccaccgcctacccttccgttcccggcaccgcctccgctgactgttctttctccgacggcgacaccgcccctctaattccccctcgccacgaagtttatggaaatggccgaatcttcgacatcacccaccgtatcaccacggcgatgccgtcgtatttctccccggacggcgtgttgggccagttcctgtggctcccccacagcatgaagaaccgctctattgcaaacatgtcagttttcaagctgcctactcacaccggcacgcatgttgattcacccggacacttcttcgatcactacctcgatgctggcttcgacgtcgatacgcttgacttggaggtgcttaatggtccagcattgttagttgatgttccgagggacaagaacataactgctgaagtgatgaagtccttaaatattccaaagggagtaggtcgtgtgctcttcagaacattaaatactgacagacggcttatgttcaaaaatcagtttgacacgagctatgtgggattcatgaaggatggagccaagtggttggtggagaacacagatatcaaacttgttggaattgattacttatctgttgctgcatttgatgatttgattacatcccaccttgtttttctagaaagcaggggccatcagctgtacctcttcctttcacacagaaacaaagaggatcctgatcagttttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

254

Amino Acids

28.42

Weight (kDa)

5.96

Isoelectric Point (pI)

27.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 390
AccB1I GGYRCC 1 cut(s) 95
AccBSI CCGCTC 1 cut(s) 285
AciI CCGC 5 cut(s) 75, 99, 105, 135, 283
AclWI GGATC 2 cut(s) 743, 756
AcoI YGGCCR 1 cut(s) 175
AcuI CTGAAG 2 cut(s) 486, 499
AcyI GRCGYC 1 cut(s) 387
AfaI GTAC 1 cut(s) 719
AflIII ACRYGT 1 cut(s) 297
AgsI TTSAA 2 cut(s) 310, 550
AluBI AGCT 3 cut(s) 313, 570, 715
AluI AGCT 3 cut(s) 313, 570, 715
Alw21I GWGCWC 1 cut(s) 513
AlwI GGATC 2 cut(s) 743, 756
AlwNI CAGNNNCTG 1 cut(s) 256
AoxI GGCC 3 cut(s) 175, 247, 706
ApeKI GCWGC 2 cut(s) 313, 656
AspS9I GGNCC 3 cut(s) 247, 419, 706
AsuC2I CCSGG 3 cut(s) 93, 233, 348
AsuHPI GGTGA 3 cut(s) 184, 196, 336
AvaII GGWCC 1 cut(s) 419
BamHI GGATCC 1 cut(s) 748
BanI GGYRCC 1 cut(s) 95
BauI CACGAG 1 cut(s) 565
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 513
BbvI GCAGC 2 cut(s) 300, 643
BccI CCATC 2 cut(s) 584, 717
BceAI ACGGC 5 cut(s) 142, 202, 225, 253, 554
BclI TGATCA 1 cut(s) 754
BcnI CCSGG 3 cut(s) 93, 233, 348
BfaI CTAG 1 cut(s) 695
BisI GCNGC 2 cut(s) 314, 657
BlsI GCNGC 2 cut(s) 315, 658
Bme1390I CCNGG 3 cut(s) 93, 233, 348
Bme18I GGWCC 1 cut(s) 419
BmgT120I GGNCC 3 cut(s) 247, 419, 706
BmiI GGNNCC 5 cut(s) 97, 263, 595, 707, 750
BmrFI CCNGG 3 cut(s) 93, 233, 348
BmsI GCATC 2 cut(s) 204, 364
BpiI GAAGAC 1 cut(s) 11
BpuMI CCSGG 3 cut(s) 93, 233, 348
BsaBI GATNNNNATC 1 cut(s) 753
BsaHI GRCGYC 1 cut(s) 387
BsaJI CCNNGG 3 cut(s) 207, 231, 443
Bse118I RCCGGY 1 cut(s) 326
Bse1I ACTGG 1 cut(s) 250
Bse8I GATNNNNATC 1 cut(s) 753
BseDI CCNNGG 3 cut(s) 207, 231, 443
BseGI GGATG 2 cut(s) 595, 677
BseJI GATNNNNATC 1 cut(s) 753
BseNI ACTGG 1 cut(s) 250
BseRI GAGGAG 1 cut(s) 11
BseXI GCAGC 2 cut(s) 300, 643
BshFI GGCC 3 cut(s) 177, 249, 708
BshNI GGYRCC 1 cut(s) 95
BsiHKAI GWGCWC 1 cut(s) 513
BsiSI CCGG 4 cut(s) 93, 233, 327, 348
BslFI GGGAC 1 cut(s) 461
BsmFI GGGAC 1 cut(s) 461
BsnI GGCC 3 cut(s) 177, 249, 708
Bsp1286I GDGCHC 1 cut(s) 513
Bsp143I GATC 3 cut(s) 361, 748, 754
BspACI CCGC 5 cut(s) 75, 99, 105, 135, 283
BspANI GGCC 3 cut(s) 177, 249, 708
BspHI TCATGA 1 cut(s) 582
BspLI GGNNCC 5 cut(s) 97, 263, 595, 707, 750
BspPI GGATC 2 cut(s) 743, 756
BspQI GCTCTTC 1 cut(s) 518
BspT107I GGYRCC 1 cut(s) 95
BsrBI CCGCTC 1 cut(s) 285
BsrFI RCCGGY 1 cut(s) 326
BsrI ACTGG 1 cut(s) 250
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 3 cut(s) 207, 231, 443
BssMI GATC 3 cut(s) 361, 748, 754
BssNI GRCGYC 1 cut(s) 387
BssSI CACGAG 1 cut(s) 565
Bst2BI CACGAG 1 cut(s) 565
Bst4CI ACNGT 2 cut(s) 113, 200
Bst6I CTCTTC 2 cut(s) 518, 728
BstACI GRCGYC 1 cut(s) 387
BstC8I GCNNGC 2 cut(s) 332, 379
BstDSI CCRYGG 1 cut(s) 207
BstF5I GGATG 2 cut(s) 595, 677
BstKTI GATC 3 cut(s) 364, 751, 757
BstMBI GATC 3 cut(s) 361, 748, 754
BstMWI GCNNNNNNNGC 2 cut(s) 74, 246
BstNSI RCATGY 2 cut(s) 301, 338
BstSCI CCNGG 3 cut(s) 91, 231, 346
BstV1I GCAGC 2 cut(s) 300, 643
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 748
BstXI CCANNNNNNTGG 1 cut(s) 604
BstYI RGATCY 1 cut(s) 748
BsuRI GGCC 3 cut(s) 177, 249, 708
BtgI CCRYGG 1 cut(s) 207
BtgZI GCGATG 1 cut(s) 227
BtsCI GGATG 2 cut(s) 595, 677
Cac8I GCNNGC 2 cut(s) 332, 379
CaiI CAGNNNCTG 1 cut(s) 256
CciI TCATGA 1 cut(s) 582
Cfr10I RCCGGY 1 cut(s) 326
Cfr13I GGNCC 3 cut(s) 247, 419, 706
Csp6I GTAC 1 cut(s) 718
CviAII CATG 4 cut(s) 274, 298, 335, 583
CviQI GTAC 1 cut(s) 718
DpnI GATC 3 cut(s) 363, 750, 756
DpnII GATC 3 cut(s) 361, 748, 754
EaeI YGGCCR 1 cut(s) 175
Eam1104I CTCTTC 2 cut(s) 518, 728
EarI CTCTTC 2 cut(s) 518, 728
Eco32I GATATC 1 cut(s) 622
Eco47I GGWCC 1 cut(s) 419
Eco57I CTGAAG 2 cut(s) 486, 499
EcoRV GATATC 1 cut(s) 622
FaeI CATG 4 cut(s) 277, 301, 338, 586
FaiI YATR 8 cut(s) 168, 275, 299, 336, 458, 545, 573, 584
FaqI GGGAC 1 cut(s) 461
FatI CATG 4 cut(s) 273, 297, 334, 582
FbaI TGATCA 1 cut(s) 754
Fnu4HI GCNGC 2 cut(s) 314, 657
FokI GGATG 2 cut(s) 602, 664
Fsp4HI GCNGC 2 cut(s) 314, 657
FspBI CTAG 1 cut(s) 695
GluI GCNGC 2 cut(s) 314, 657
HaeIII GGCC 3 cut(s) 177, 249, 708
HapII CCGG 4 cut(s) 93, 233, 327, 348
Hin1I GRCGYC 1 cut(s) 387
Hin1II CATG 4 cut(s) 277, 301, 338, 586
HinfI GANTC 3 cut(s) 180, 341, 579
HpaII CCGG 4 cut(s) 93, 233, 327, 348
HphI GGTGA 3 cut(s) 184, 196, 336
Hpy188I TCNGA 3 cut(s) 124, 444, 518
Hpy188III TCNNGA 3 cut(s) 583, 695, 752
Hpy99I CGWCG 4 cut(s) 128, 223, 389, 392
HpyAV CCTTC 2 cut(s) 93, 580
HpyCH4III ACNGT 2 cut(s) 113, 200
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 2 cut(s) 293, 659
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 246
HpySE526I ACGT 1 cut(s) 387
Hsp92I GRCGYC 1 cut(s) 387
Hsp92II CATG 4 cut(s) 277, 301, 338, 586
Ksp22I TGATCA 1 cut(s) 754
Kzo9I GATC 3 cut(s) 361, 748, 754
LguI GCTCTTC 1 cut(s) 518
LmnI GCTCC 3 cut(s) 38, 267, 593
LpnPI CCDG 9 cut(s) 106, 246, 263, 269, 340, 361, 363, 435, 688
Lsp1109I GCAGC 2 cut(s) 300, 643
LweI GCATC 2 cut(s) 204, 364
MaeI CTAG 1 cut(s) 695
MaeII ACGT 1 cut(s) 387
MalI GATC 3 cut(s) 363, 750, 756
MbiI CCGCTC 1 cut(s) 285
MboI GATC 3 cut(s) 361, 748, 754
MboII GAAGA 6 cut(s) 16, 175, 289, 349, 505, 715
MflI RGATCY 1 cut(s) 748
MhlI GDGCHC 1 cut(s) 513
MluCI AATT 2 cut(s) 144, 636
MmeI TCCRAC 2 cut(s) 147, 613
MnlI CCTC 9 cut(s) 32, 112, 150, 162, 380, 400, 438, 731, 739
MseI TTAA 3 cut(s) 414, 482, 524
MslI CAYNNNNRTG 1 cut(s) 212
MspA1I CMGCKG 2 cut(s) 107, 715
MspI CCGG 4 cut(s) 93, 233, 327, 348
MspR9I CCNGG 3 cut(s) 93, 233, 348
MwoI GCNNNNNNNGC 2 cut(s) 74, 246
NciI CCSGG 3 cut(s) 93, 233, 348
NdeII GATC 3 cut(s) 361, 748, 754
NlaIII CATG 4 cut(s) 277, 301, 338, 586
NlaIV GGNNCC 5 cut(s) 97, 263, 595, 707, 750
NspI RCATGY 2 cut(s) 301, 338
PagI TCATGA 1 cut(s) 582
PciI ACATGT 1 cut(s) 297
PciSI GCTCTTC 1 cut(s) 518
PcsI WCGNNNNNNNCGW 1 cut(s) 215
PfeI GAWTC 3 cut(s) 180, 341, 579
PkrI GCNGC 2 cut(s) 315, 658
PscI ACATGT 1 cut(s) 297
PspN4I GGNNCC 5 cut(s) 97, 263, 595, 707, 750
PspPI GGNCC 3 cut(s) 247, 419, 706
PsrI GAACNNNNNNTAC 2 cut(s) 511, 543
PstNI CAGNNNCTG 1 cut(s) 256
PsuI RGATCY 1 cut(s) 748
PvuII CAGCTG 1 cut(s) 715
RsaI GTAC 1 cut(s) 719
RsaNI GTAC 1 cut(s) 718
RseI CAYNNNNRTG 1 cut(s) 212
SapI GCTCTTC 1 cut(s) 518
SaqAI TTAA 3 cut(s) 414, 482, 524
SatI GCNGC 2 cut(s) 314, 657
Sau3AI GATC 3 cut(s) 361, 748, 754
Sau96I GGNCC 3 cut(s) 247, 419, 706
ScrFI CCNGG 3 cut(s) 93, 233, 348
SduI GDGCHC 1 cut(s) 513
SfaNI GCATC 2 cut(s) 204, 364
SinI GGWCC 1 cut(s) 419
SmiMI CAYNNNNRTG 1 cut(s) 212
Sse9I AATT 2 cut(s) 144, 636
SsiI CCGC 5 cut(s) 75, 99, 105, 135, 283
SspI AATATT 1 cut(s) 487
SspMI CTAG 1 cut(s) 695
StyD4I CCNGG 3 cut(s) 91, 231, 346
TaaI ACNGT 2 cut(s) 113, 200
TaiI ACGT 1 cut(s) 390
TaqI TCGA 5 cut(s) 186, 360, 372, 384, 390
TasI AATT 2 cut(s) 144, 636
TfiI GAWTC 3 cut(s) 180, 341, 579
Tru1I TTAA 3 cut(s) 414, 482, 524
Tru9I TTAA 3 cut(s) 414, 482, 524
TseI GCWGC 2 cut(s) 313, 656
TspDTI ATGAA 5 cut(s) 17, 290, 488, 571, 599
TspGWI ACGGA 1 cut(s) 76
VpaK11BI GGWCC 1 cut(s) 419
XbaI TCTAGA 1 cut(s) 694
XceI RCATGY 2 cut(s) 301, 338
XcmI CCANNNNNNNNNTGG 2 cut(s) 164, 604
XspI CTAG 1 cut(s) 695
ZraI GACGTC 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.