Rw6G006780

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000645
Physical Location & Seq
Forward (+)
10853919 .. 10856991
3073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G006780.1

Sequence Viewer

Length: 786 bp
ATGAACCGACCCCTTCTCGATCCCCTCTTTCTCACATTTTCTTGCTTATTCTTCCTTGCTTCCTCCTCCTCCTCCTCAGGCTACGGTCCCGGCGACACTCCAAAACCCGTTAGGAGGGAAGTATACGGCAATGGGAGAATCTTTGACATCACCCACAGGTACACCCCGGAGATTCCCGTGTATGGTTCAAAGCTTGGGATCGGACATTTCCGGTGGCTCCAACTGAGCATGAAGAATGGTTCACTTACCAACAACTCGGAATTCAAGCTGCCTGTTCACACCGGAACCCATGTTGATGCCCCTGGCCATGTGTTTGATGATTACTATGATGCTGGGTTTGATGTAGATCAGCTTGACTTGGAGGTACTCAATGGCCCTGCACTTGTTGTTGATGTTCCCAGGGATAGCAACATAACTGCTGAAGTGATGGAGTCCTTAAATATTCCCAAGAGTACTCGCCGAGTACTTTTCAGGACATTAAATACTGATAGGGGTCTTATGCATAAAAAGGAATTTGACTCAAGCTTTGTGGGATTCACGGAGGATGGAGCGAAATGGGTGGTTAAGAACACGGACATCAAACTTGTTGGAACTGATTACTTTTCCATTGCTACCTATGATGAAGCTATTCCGGCACATATTGCTTTTCTAAAGGGAAGGGAAATCATACCTGTGGAAGGCCTAAAGCTTGATGGCATAAAACCAGGAAATTATTCTGTGCATTGCTTACCTCTAAGGTTGCTTGGTGCTGAAGGAGCACCAACAAGATGCATTCTCATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

261

Amino Acids

28.98

Weight (kDa)

6.24

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 48 - 203 3.6e-21 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 123
AclWI GGATC 2 cut(s) 14, 206
AcoI YGGCCR 1 cut(s) 304
AcsI RAATTY 2 cut(s) 260, 512
AcuI CTGAAG 2 cut(s) 441, 771
AfaI GTAC 4 cut(s) 161, 366, 454, 465
AfiI CCNNNNNNNGG 3 cut(s) 114, 182, 677
AgsI TTSAA 2 cut(s) 189, 265
AjnI CCWGG 3 cut(s) 301, 398, 703
AluBI AGCT 6 cut(s) 193, 268, 352, 525, 626, 688
AluI AGCT 6 cut(s) 193, 268, 352, 525, 626, 688
Alw21I GWGCWC 1 cut(s) 760
AlwI GGATC 2 cut(s) 14, 206
AoxI GGCC 3 cut(s) 304, 373, 679
ApeKI GCWGC 1 cut(s) 268
ApoI RAATTY 2 cut(s) 260, 512
ArsI GACNNNNNNTTYG 2 cut(s) 509, 541
Asp700I GAANNNNTTC 2 cut(s) 627, 712
AspS9I GGNCC 2 cut(s) 86, 374
AsuC2I CCSGG 2 cut(s) 90, 167
AsuHPI GGTGA 1 cut(s) 142
AvaII GGWCC 1 cut(s) 86
AxyI CCTNAGG 1 cut(s) 76
BalI TGGCCA 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 760
BbvI GCAGC 1 cut(s) 255
BccI CCATC 3 cut(s) 421, 539, 686
BceAI ACGGC 1 cut(s) 142
BciT130I CCWGG 3 cut(s) 303, 400, 705
BcnI CCSGG 2 cut(s) 90, 167
BisI GCNGC 1 cut(s) 269
BlsI GCNGC 1 cut(s) 270
BmcAI AGTACT 2 cut(s) 454, 465
Bme1390I CCNGG 5 cut(s) 90, 167, 303, 400, 705
Bme18I GGWCC 1 cut(s) 86
BmgT120I GGNCC 2 cut(s) 86, 374
BmiI GGNNCC 3 cut(s) 88, 218, 286
BmrFI CCNGG 5 cut(s) 90, 167, 303, 400, 705
BmsI GCATC 3 cut(s) 286, 319, 758
BpuEI CTTGAG 1 cut(s) 505
BpuMI CCSGG 2 cut(s) 90, 167
BsaBI GATNNNNATC 1 cut(s) 345
BsaJI CCNNGG 4 cut(s) 165, 301, 398, 399
BsaWI WCCGGW 2 cut(s) 210, 281
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 182, 677
Bse21I CCTNAGG 1 cut(s) 76
Bse3DI GCAATG 3 cut(s) 136, 606, 721
Bse8I GATNNNNATC 1 cut(s) 345
BseBI CCWGG 3 cut(s) 303, 400, 705
BseDI CCNNGG 4 cut(s) 165, 301, 398, 399
BseGI GGATG 1 cut(s) 550
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 3 cut(s) 114, 182, 677
BseMI GCAATG 3 cut(s) 136, 606, 721
BseMII CTCAG 2 cut(s) 90, 215
BseRI GAGGAG 4 cut(s) 55, 58, 61, 64
BseXI GCAGC 1 cut(s) 255
BseYI CCCAGC 1 cut(s) 332
BsgI GTGCAG 1 cut(s) 363
BshFI GGCC 3 cut(s) 306, 375, 681
BsiHKAI GWGCWC 1 cut(s) 760
BsiSI CCGG 5 cut(s) 90, 167, 211, 282, 632
BslFI GGGAC 1 cut(s) 72
BslI CCNNNNNNNGG 3 cut(s) 114, 182, 677
BsmFI GGGAC 1 cut(s) 72
BsmI GAATGC 1 cut(s) 771
BsnI GGCC 3 cut(s) 306, 375, 681
Bsp1286I GDGCHC 1 cut(s) 760
Bsp143I GATC 3 cut(s) 19, 198, 346
BspANI GGCC 3 cut(s) 306, 375, 681
BspCNI CTCAG 2 cut(s) 89, 216
BspLI GGNNCC 3 cut(s) 88, 218, 286
BspPI GGATC 2 cut(s) 14, 206
BsrDI GCAATG 3 cut(s) 136, 606, 721
BssECI CCNNGG 4 cut(s) 165, 301, 398, 399
BssMI GATC 3 cut(s) 19, 198, 346
BssNAI GTATAC 1 cut(s) 124
Bst1107I GTATAC 1 cut(s) 124
Bst2UI CCWGG 3 cut(s) 303, 400, 705
Bst4CI ACNGT 1 cut(s) 86
BstAPI GCANNNNNTGC 1 cut(s) 641
BstDEI CTNAG 3 cut(s) 76, 224, 734
BstENI CCTNNNNNAGG 1 cut(s) 675
BstF5I GGATG 1 cut(s) 550
BstKTI GATC 3 cut(s) 22, 201, 349
BstMBI GATC 3 cut(s) 19, 198, 346
BstMWI GCNNNNNNNGC 3 cut(s) 632, 641, 755
BstNI CCWGG 3 cut(s) 303, 400, 705
BstSCI CCNGG 5 cut(s) 88, 165, 301, 398, 703
BstV1I GCAGC 1 cut(s) 255
BstZ17I GTATAC 1 cut(s) 124
Bsu36I CCTNAGG 1 cut(s) 76
BsuRI GGCC 3 cut(s) 306, 375, 681
BtsCI GGATG 1 cut(s) 550
Cfr13I GGNCC 2 cut(s) 86, 374
Csp6I GTAC 4 cut(s) 160, 365, 453, 464
CspCI CAANNNNNGTGG 2 cut(s) 510, 545
CviAII CATG 3 cut(s) 229, 290, 308
CviQI GTAC 4 cut(s) 160, 365, 453, 464
DdeI CTNAG 3 cut(s) 76, 224, 734
DpnI GATC 3 cut(s) 21, 200, 348
DpnII GATC 3 cut(s) 19, 198, 346
EaeI YGGCCR 1 cut(s) 304
Eco147I AGGCCT 1 cut(s) 681
Eco47I GGWCC 1 cut(s) 86
Eco57I CTGAAG 2 cut(s) 441, 771
Eco81I CCTNAGG 1 cut(s) 76
EcoNI CCTNNNNNAGG 1 cut(s) 675
EcoRI GAATTC 1 cut(s) 260
EcoRII CCWGG 3 cut(s) 301, 398, 703
EcoT22I ATGCAT 2 cut(s) 504, 773
FaeI CATG 3 cut(s) 232, 293, 311
FaqI GGGAC 1 cut(s) 72
FatI CATG 3 cut(s) 228, 289, 307
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 1 cut(s) 269
FokI GGATG 1 cut(s) 557
Fsp4HI GCNGC 1 cut(s) 269
GluI GCNGC 1 cut(s) 269
GsaI CCCAGC 1 cut(s) 336
HaeIII GGCC 3 cut(s) 306, 375, 681
HapII CCGG 5 cut(s) 90, 167, 211, 282, 632
Hin1II CATG 3 cut(s) 232, 293, 311
HindIII AAGCTT 3 cut(s) 191, 523, 686
HinfI GANTC 5 cut(s) 138, 172, 431, 518, 534
HpaII CCGG 5 cut(s) 90, 167, 211, 282, 632
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 4 cut(s) 124, 162, 242, 277
Hpy188I TCNGA 2 cut(s) 203, 259
Hpy188III TCNNGA 2 cut(s) 17, 472
Hpy8I GTNNAC 4 cut(s) 124, 162, 242, 277
HpyAV CCTTC 4 cut(s) 23, 651, 671, 746
HpyCH4III ACNGT 1 cut(s) 86
HpyCH4V TGCA 4 cut(s) 380, 502, 721, 771
HpyF10VI GCNNNNNNNGC 3 cut(s) 632, 641, 755
HpyF3I CTNAG 3 cut(s) 76, 224, 734
Hsp92II CATG 3 cut(s) 232, 293, 311
Kzo9I GATC 3 cut(s) 19, 198, 346
LmnI GCTCC 3 cut(s) 222, 548, 755
Lsp1109I GCAGC 1 cut(s) 255
LweI GCATC 3 cut(s) 286, 319, 758
MalI GATC 3 cut(s) 21, 200, 348
MboI GATC 3 cut(s) 19, 198, 346
MboII GAAGA 2 cut(s) 43, 244
MhlI GDGCHC 1 cut(s) 760
MlsI TGGCCA 1 cut(s) 306
MluCI AATT 3 cut(s) 260, 512, 709
MluNI TGGCCA 1 cut(s) 306
MlyI GAGTC 2 cut(s) 440, 512
MmeI TCCRAC 2 cut(s) 244, 568
Mox20I TGGCCA 1 cut(s) 306
Mph1103I ATGCAT 2 cut(s) 504, 773
MroXI GAANNNNTTC 2 cut(s) 627, 712
MscI TGGCCA 1 cut(s) 306
MseI TTAA 3 cut(s) 437, 479, 564
MslI CAYNNNNRTG 2 cut(s) 294, 671
Msp20I TGGCCA 1 cut(s) 306
MspI CCGG 5 cut(s) 90, 167, 211, 282, 632
MspR9I CCNGG 5 cut(s) 90, 167, 303, 400, 705
Mva1269I GAATGC 1 cut(s) 771
MvaI CCWGG 3 cut(s) 303, 400, 705
MwoI GCNNNNNNNGC 3 cut(s) 632, 641, 755
NciI CCSGG 2 cut(s) 90, 167
NdeII GATC 3 cut(s) 19, 198, 346
NlaIII CATG 3 cut(s) 232, 293, 311
NlaIV GGNNCC 3 cut(s) 88, 218, 286
NmeAIII GCCGAG 1 cut(s) 485
NsiI ATGCAT 2 cut(s) 504, 773
PasI CCCWGGG 1 cut(s) 399
PceI AGGCCT 1 cut(s) 681
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 1 cut(s) 771
PdmI GAANNNNTTC 2 cut(s) 627, 712
PfeI GAWTC 3 cut(s) 138, 172, 534
PkrI GCNGC 1 cut(s) 270
PleI GAGTC 2 cut(s) 439, 512
PpsI GAGTC 2 cut(s) 439, 512
Psp6I CCWGG 3 cut(s) 301, 398, 703
PspFI CCCAGC 1 cut(s) 332
PspGI CCWGG 3 cut(s) 301, 398, 703
PspN4I GGNNCC 3 cut(s) 88, 218, 286
PspPI GGNCC 2 cut(s) 86, 374
RsaI GTAC 4 cut(s) 161, 366, 454, 465
RsaNI GTAC 4 cut(s) 160, 365, 453, 464
RseI CAYNNNNRTG 2 cut(s) 294, 671
SaqAI TTAA 3 cut(s) 437, 479, 564
SatI GCNGC 1 cut(s) 269
Sau3AI GATC 3 cut(s) 19, 198, 346
Sau96I GGNCC 2 cut(s) 86, 374
ScaI AGTACT 2 cut(s) 454, 465
SchI GAGTC 2 cut(s) 440, 512
ScrFI CCNGG 5 cut(s) 90, 167, 303, 400, 705
SduI GDGCHC 1 cut(s) 760
SfaNI GCATC 3 cut(s) 286, 319, 758
SinI GGWCC 1 cut(s) 86
SmiMI CAYNNNNRTG 2 cut(s) 294, 671
SmlI CTYRAG 1 cut(s) 520
SmoI CTYRAG 1 cut(s) 520
Sse9I AATT 3 cut(s) 260, 512, 709
SseBI AGGCCT 1 cut(s) 681
SspI AATATT 1 cut(s) 442
StuI AGGCCT 1 cut(s) 681
StyD4I CCNGG 5 cut(s) 88, 165, 301, 398, 703
TaaI ACNGT 1 cut(s) 86
TaqI TCGA 1 cut(s) 18
TasI AATT 3 cut(s) 260, 512, 709
TatI WGTACW 2 cut(s) 452, 463
TfiI GAWTC 3 cut(s) 138, 172, 534
Tru1I TTAA 3 cut(s) 437, 479, 564
Tru9I TTAA 3 cut(s) 437, 479, 564
TseI GCWGC 1 cut(s) 268
TspDTI ATGAA 3 cut(s) 17, 245, 636
TspGWI ACGGA 2 cut(s) 554, 587
VpaK11BI GGWCC 1 cut(s) 86
XagI CCTNNNNNAGG 1 cut(s) 675
XapI RAATTY 2 cut(s) 260, 512
XmiI GTMKAC 1 cut(s) 123
XmnI GAANNNNTTC 2 cut(s) 627, 712
ZrmI AGTACT 2 cut(s) 454, 465
Zsp2I ATGCAT 2 cut(s) 504, 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.