FvH4_2g05380

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000645
Physical Location & Seq
Forward (+)
4436977 .. 4440976
4000 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05380.t1

Sequence Viewer

Length: 816 bp
ATGAATCCAATCCTTCTCCTCCTCTTCTTCCTCTGTTCCGTTTTCGCTACCCTCTCCCACGCCACCACAGCCTACCCTTCCATTCCGGGCACCACCCCCGTCGATGTCTCTGCCGGCGCCCCTACCGCCCTTATCCCCCCTCGCCGGGAAGTCTACGGTAACGGCCGAATATTCGACATCAGCCACCGATACAGCCCCGACATGCCGGCGTTTGACTCCGTCGACGGCGTCGGCCAGTTCCTGTGGCTCCCCAACAGCATGAAGAACGGCTCCATTGCTAACAACTCCGAGATGAAGCTCCCCACTCACACTGGGACCCACGTCGACGCCCCCGGCCACGTCTTCGATCACTACTTCGACGCCGGCTTCGACGTCGACACCCTCGACTTGGATGTCCTTAATGGTGCTGCGTTGTTAGTTGACGTTCCAAGGGATAAGAACATTACTGCTGAAGTCATGGAGGGCTTACATATCCCCAAGGGGGTGAGTCGTGTACTTTTCAGAACATTGAACACAGACAGACGGCTTATGTTCCAAAAGGAGTTTGACAGCAGCTATGTCGGATTTATGAAGGATGGAGCTAAGTGGTTGGTGGAGAACACTGACATCAAACTTATTGGAGTTGATTACTTATCTGCTGCTGCATATGATGATTTGGTTCCATCTCACCTTGTTTTGCTAGAAGGCAGGGAAATCATTCTGGTAGAAGGTCTAAAACTTGATGACATCCAACCAGGAATTTATTCTGTCCATTGCTTGCCTTTGAGGCTGACCGGTGCCGAAGGATCACCAATCAGATGCATTCTTATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

272

Amino Acids

29.75

Weight (kDa)

5.17

Isoelectric Point (pI)

31.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 58 - 213 3.2e-21 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 392
AatII GACGTC 1 cut(s) 375
AccB1I GGYRCC 3 cut(s) 89, 116, 776
AccI GTMKAC 4 cut(s) 153, 222, 324, 375
AciI CCGC 1 cut(s) 126
AclWI GGATC 1 cut(s) 793
AcoI YGGCCR 3 cut(s) 163, 232, 334
AcsI RAATTY 1 cut(s) 738
AcuI CTGAAG 1 cut(s) 471
AcyI GRCGYC 5 cut(s) 117, 228, 327, 360, 372
AfaI GTAC 1 cut(s) 495
AfiI CCNNNNNNNGG 4 cut(s) 144, 145, 388, 481
AgeI ACCGGT 1 cut(s) 773
AgsI TTSAA 1 cut(s) 511
AjiI CACGTC 2 cut(s) 322, 340
AjnI CCWGG 1 cut(s) 733
AluBI AGCT 3 cut(s) 298, 555, 581
AluI AGCT 3 cut(s) 298, 555, 581
Alw26I GTCTC 1 cut(s) 112
AlwI GGATC 1 cut(s) 793
AlwNI CAGNNNCTG 1 cut(s) 241
AoxI GGCC 3 cut(s) 163, 232, 334
ApeKI GCWGC 4 cut(s) 407, 552, 638, 641
ApoI RAATTY 1 cut(s) 738
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AsiGI ACCGGT 1 cut(s) 773
Asp700I GAANNNNTTC 2 cut(s) 696, 742
AspLEI GCGC 1 cut(s) 119
AspS9I GGNCC 1 cut(s) 315
AsuC2I CCSGG 3 cut(s) 87, 146, 333
AsuHPI GGTGA 3 cut(s) 496, 659, 780
AvaII GGWCC 1 cut(s) 315
BaeGI GKGCMC 1 cut(s) 92
BanI GGYRCC 3 cut(s) 89, 116, 776
BbsI GAAGAC 1 cut(s) 334
BbvI GCAGC 4 cut(s) 394, 564, 625, 628
BccI CCATC 2 cut(s) 569, 670
BceAI ACGGC 4 cut(s) 178, 241, 283, 539
BcgI CGANNNNNNTGC 4 cut(s) 92, 126, 541, 575
BciT130I CCWGG 1 cut(s) 735
BcnI CCSGG 3 cut(s) 87, 146, 333
BcoDI GTCTC 1 cut(s) 112
BfaI CTAG 1 cut(s) 680
BfoI RGCGCY 1 cut(s) 120
BglI GCCNNNNNGGC 1 cut(s) 766
BisI GCNGC 4 cut(s) 408, 553, 639, 642
BlsI GCNGC 4 cut(s) 409, 554, 640, 643
Bme1390I CCNGG 4 cut(s) 87, 146, 333, 735
Bme18I GGWCC 1 cut(s) 315
BmgBI CACGTC 2 cut(s) 322, 340
BmgT120I GGNCC 1 cut(s) 315
BmiI GGNNCC 8 cut(s) 91, 118, 248, 271, 316, 317, 660, 778
BmrFI CCNGG 4 cut(s) 87, 146, 333, 735
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 1 cut(s) 788
BmuI ACTGGG 1 cut(s) 321
BoxI GACNNNNGTC 1 cut(s) 320
BpiI GAAGAC 1 cut(s) 334
BpuMI CCSGG 3 cut(s) 87, 146, 333
BsaHI GRCGYC 5 cut(s) 117, 228, 327, 360, 372
BsaJI CCNNGG 3 cut(s) 331, 428, 477
BsaWI WCCGGW 1 cut(s) 773
Bsc4I CCNNNNNNNGG 4 cut(s) 144, 145, 388, 481
Bse118I RCCGGY 4 cut(s) 113, 205, 362, 773
Bse1I ACTGG 2 cut(s) 235, 316
Bse3DI GCAATG 2 cut(s) 273, 751
BseBI CCWGG 1 cut(s) 735
BseDI CCNNGG 3 cut(s) 331, 428, 477
BseGI GGATG 3 cut(s) 397, 580, 726
BseLI CCNNNNNNNGG 4 cut(s) 144, 145, 388, 481
BseMI GCAATG 2 cut(s) 273, 751
BseNI ACTGG 2 cut(s) 235, 316
BseRI GAGGAG 2 cut(s) 8, 11
BseSI GKGCMC 1 cut(s) 92
BseX3I CGGCCG 1 cut(s) 163
BseXI GCAGC 4 cut(s) 394, 564, 625, 628
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 3 cut(s) 165, 234, 336
BshNI GGYRCC 3 cut(s) 89, 116, 776
BshTI ACCGGT 1 cut(s) 773
BsiEI CGRYCG 1 cut(s) 166
BsiSI CCGG 7 cut(s) 86, 114, 145, 206, 333, 363, 774
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 4 cut(s) 144, 145, 388, 481
BsmAI GTCTC 1 cut(s) 112
BsmFI GGGAC 1 cut(s) 328
BsmI GAATGC 1 cut(s) 801
BsnI GGCC 3 cut(s) 165, 234, 336
Bsp1286I GDGCHC 1 cut(s) 92
Bsp143I GATC 2 cut(s) 346, 785
BspACI CCGC 1 cut(s) 126
BspANI GGCC 3 cut(s) 165, 234, 336
BspLI GGNNCC 8 cut(s) 91, 118, 248, 271, 316, 317, 660, 778
BspPI GGATC 1 cut(s) 793
BspT107I GGYRCC 3 cut(s) 89, 116, 776
BsrDI GCAATG 2 cut(s) 273, 751
BsrFI RCCGGY 4 cut(s) 113, 205, 362, 773
BsrI ACTGG 2 cut(s) 235, 316
BssAI RCCGGY 4 cut(s) 113, 205, 362, 773
BssECI CCNNGG 3 cut(s) 331, 428, 477
BssMI GATC 2 cut(s) 346, 785
BssNI GRCGYC 5 cut(s) 117, 228, 327, 360, 372
BssT1I CCWWGG 2 cut(s) 428, 477
Bst2UI CCWGG 1 cut(s) 735
Bst4CI ACNGT 1 cut(s) 158
Bst6I CTCTTC 1 cut(s) 29
BstACI GRCGYC 5 cut(s) 117, 228, 327, 360, 372
BstC8I GCNNGC 4 cut(s) 115, 207, 364, 758
BstDEI CTNAG 1 cut(s) 582
BstF5I GGATG 3 cut(s) 397, 580, 726
BstH2I RGCGCY 1 cut(s) 120
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 2 cut(s) 349, 788
BstMAI GTCTC 1 cut(s) 112
BstMBI GATC 2 cut(s) 346, 785
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 3 cut(s) 68, 125, 766
BstNI CCWGG 1 cut(s) 735
BstNSI RCATGY 1 cut(s) 205
BstPAI GACNNNNGTC 1 cut(s) 320
BstSCI CCNGG 4 cut(s) 85, 144, 331, 733
BstSLI GKGCMC 1 cut(s) 92
BstV1I GCAGC 4 cut(s) 394, 564, 625, 628
BstV2I GAAGAC 1 cut(s) 334
BstZI CGGCCG 1 cut(s) 163
BsuRI GGCC 3 cut(s) 165, 234, 336
BtrI CACGTC 2 cut(s) 322, 340
BtsCI GGATG 3 cut(s) 397, 580, 726
BtsIMutI CAGTG 2 cut(s) 309, 600
Cac8I GCNNGC 4 cut(s) 115, 207, 364, 758
CaiI CAGNNNCTG 1 cut(s) 241
CfoI GCGC 1 cut(s) 119
Cfr10I RCCGGY 4 cut(s) 113, 205, 362, 773
Cfr13I GGNCC 1 cut(s) 315
CseI GACGC 3 cut(s) 217, 335, 368
Csp6I GTAC 1 cut(s) 494
CspAI ACCGGT 1 cut(s) 773
CviAII CATG 3 cut(s) 202, 259, 457
CviQI GTAC 1 cut(s) 494
DdeI CTNAG 1 cut(s) 582
DinI GGCGCC 1 cut(s) 118
DpnI GATC 2 cut(s) 348, 787
DpnII GATC 2 cut(s) 346, 785
DrdI GACNNNNNNGTC 1 cut(s) 392
DseDI GACNNNNNNGTC 1 cut(s) 392
EaeI YGGCCR 3 cut(s) 163, 232, 334
EagI CGGCCG 1 cut(s) 163
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
EclXI CGGCCG 1 cut(s) 163
Eco130I CCWWGG 2 cut(s) 428, 477
Eco47I GGWCC 1 cut(s) 315
Eco52I CGGCCG 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 471
EcoO109I RGGNCCY 1 cut(s) 315
EcoRII CCWGG 1 cut(s) 733
EcoT14I CCWWGG 2 cut(s) 428, 477
EcoT22I ATGCAT 1 cut(s) 803
EgeI GGCGCC 1 cut(s) 118
EheI GGCGCC 1 cut(s) 118
ErhI CCWWGG 2 cut(s) 428, 477
FaeI CATG 3 cut(s) 205, 262, 460
FaiI YATR 9 cut(s) 203, 260, 458, 471, 530, 558, 569, 646, 648
FaqI GGGAC 1 cut(s) 328
FatI CATG 3 cut(s) 201, 258, 456
FauNDI CATATG 1 cut(s) 646
FblI GTMKAC 4 cut(s) 153, 222, 324, 375
Fnu4HI GCNGC 4 cut(s) 408, 553, 639, 642
FokI GGATG 3 cut(s) 404, 587, 713
Fsp4HI GCNGC 4 cut(s) 408, 553, 639, 642
FspBI CTAG 1 cut(s) 680
GlaI GCGC 1 cut(s) 118
GluI GCNGC 4 cut(s) 408, 553, 639, 642
HaeII RGCGCY 1 cut(s) 120
HaeIII GGCC 3 cut(s) 165, 234, 336
HapII CCGG 7 cut(s) 86, 114, 145, 206, 333, 363, 774
HgaI GACGC 3 cut(s) 217, 335, 368
HhaI GCGC 1 cut(s) 119
Hin1I GRCGYC 5 cut(s) 117, 228, 327, 360, 372
Hin1II CATG 3 cut(s) 205, 262, 460
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HincII GTYRAC 4 cut(s) 223, 325, 376, 421
HindII GTYRAC 4 cut(s) 223, 325, 376, 421
HinfI GANTC 3 cut(s) 4, 215, 487
HpaII CCGG 7 cut(s) 86, 114, 145, 206, 333, 363, 774
HphI GGTGA 3 cut(s) 496, 659, 780
Hpy166II GTNNAC 6 cut(s) 154, 223, 325, 376, 421, 494
Hpy188I TCNGA 4 cut(s) 289, 503, 563, 797
Hpy8I GTNNAC 6 cut(s) 154, 223, 325, 376, 421, 494
Hpy99I CGWCG 9 cut(s) 104, 224, 227, 233, 326, 329, 362, 374, 377
HpyAV CCTTC 6 cut(s) 23, 87, 565, 677, 701, 776
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4IV ACGT 4 cut(s) 321, 339, 372, 423
HpyCH4V TGCA 2 cut(s) 644, 801
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 125, 766
HpyF3I CTNAG 1 cut(s) 582
HpySE526I ACGT 4 cut(s) 321, 339, 372, 423
Hsp92I GRCGYC 5 cut(s) 117, 228, 327, 360, 372
Hsp92II CATG 3 cut(s) 205, 262, 460
HspAI GCGC 1 cut(s) 117
KasI GGCGCC 1 cut(s) 116
KflI GGGWCCC 1 cut(s) 315
KroI GCCGGC 3 cut(s) 113, 205, 362
KroNI GCCGGC 3 cut(s) 115, 207, 364
Kzo9I GATC 2 cut(s) 346, 785
LmnI GCTCC 4 cut(s) 252, 275, 303, 578
Lsp1109I GCAGC 4 cut(s) 394, 564, 625, 628
LweI GCATC 1 cut(s) 788
MaeI CTAG 1 cut(s) 680
MaeII ACGT 4 cut(s) 321, 339, 372, 423
MaeIII GTNAC 1 cut(s) 158
MalI GATC 2 cut(s) 348, 787
MboI GATC 2 cut(s) 346, 785
MboII GAAGA 4 cut(s) 16, 19, 274, 334
MhlI GDGCHC 1 cut(s) 92
MluCI AATT 1 cut(s) 738
Mly113I GGCGCC 1 cut(s) 117
MlyI GAGTC 2 cut(s) 209, 496
MmeI TCCRAC 2 cut(s) 541, 754
MnlI CCTC 8 cut(s) 29, 32, 41, 62, 150, 392, 454, 759
Mph1103I ATGCAT 1 cut(s) 803
MroNI GCCGGC 3 cut(s) 113, 205, 362
MroXI GAANNNNTTC 2 cut(s) 696, 742
MseI TTAA 1 cut(s) 399
MspI CCGG 7 cut(s) 86, 114, 145, 206, 333, 363, 774
MspR9I CCNGG 4 cut(s) 87, 146, 333, 735
Mva1269I GAATGC 1 cut(s) 801
MvaI CCWGG 1 cut(s) 735
MwoI GCNNNNNNNGC 3 cut(s) 68, 125, 766
NaeI GCCGGC 3 cut(s) 115, 207, 364
NarI GGCGCC 1 cut(s) 117
NciI CCSGG 3 cut(s) 87, 146, 333
NdeI CATATG 1 cut(s) 646
NdeII GATC 2 cut(s) 346, 785
NgoMIV GCCGGC 3 cut(s) 113, 205, 362
NlaIII CATG 3 cut(s) 205, 262, 460
NlaIV GGNNCC 8 cut(s) 91, 118, 248, 271, 316, 317, 660, 778
NsiI ATGCAT 1 cut(s) 803
NspI RCATGY 1 cut(s) 205
PcsI WCGNNNNNNNCGW 2 cut(s) 366, 381
PctI GAATGC 1 cut(s) 801
PdiI GCCGGC 3 cut(s) 115, 207, 364
PdmI GAANNNNTTC 2 cut(s) 696, 742
PfeI GAWTC 1 cut(s) 4
PflFI GACNNNGTC 2 cut(s) 218, 227
PinAI ACCGGT 1 cut(s) 773
PkrI GCNGC 4 cut(s) 409, 554, 640, 643
PleI GAGTC 2 cut(s) 209, 495
PluTI GGCGCC 1 cut(s) 120
PpsI GAGTC 2 cut(s) 209, 495
PpuMI RGGWCCY 1 cut(s) 315
PshAI GACNNNNGTC 1 cut(s) 320
Psp5II RGGWCCY 1 cut(s) 315
Psp6I CCWGG 1 cut(s) 733
PspGI CCWGG 1 cut(s) 733
PspN4I GGNNCC 8 cut(s) 91, 118, 248, 271, 316, 317, 660, 778
PspPI GGNCC 1 cut(s) 315
PspPPI RGGWCCY 1 cut(s) 315
PstNI CAGNNNCTG 1 cut(s) 241
PsyI GACNNNGTC 2 cut(s) 218, 227
RsaI GTAC 1 cut(s) 495
RsaNI GTAC 1 cut(s) 494
SalI GTCGAC 3 cut(s) 221, 323, 374
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 4 cut(s) 408, 553, 639, 642
Sau3AI GATC 2 cut(s) 346, 785
Sau96I GGNCC 1 cut(s) 315
SchI GAGTC 2 cut(s) 209, 496
ScrFI CCNGG 4 cut(s) 87, 146, 333, 735
SduI GDGCHC 1 cut(s) 92
SetI ASST 9 cut(s) 300, 324, 342, 375, 426, 557, 583, 672, 712
SfaNI GCATC 1 cut(s) 788
SfoI GGCGCC 1 cut(s) 118
SgrDI CGTCGACG 2 cut(s) 221, 323
SinI GGWCC 1 cut(s) 315
Sse9I AATT 1 cut(s) 738
SsiI CCGC 1 cut(s) 126
SspDI GGCGCC 1 cut(s) 116
SspI AATATT 1 cut(s) 171
SspMI CTAG 1 cut(s) 680
StyD4I CCNGG 4 cut(s) 85, 144, 331, 733
StyI CCWWGG 2 cut(s) 428, 477
TaaI ACNGT 1 cut(s) 158
TaiI ACGT 4 cut(s) 324, 342, 375, 426
TaqI TCGA 9 cut(s) 102, 174, 222, 324, 345, 357, 369, 375, 384
TasI AATT 1 cut(s) 738
TatI WGTACW 1 cut(s) 493
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 2 cut(s) 316, 607
TseI GCWGC 4 cut(s) 407, 552, 638, 641
TspDTI ATGAA 4 cut(s) 17, 275, 308, 584
TspGWI ACGGA 2 cut(s) 28, 208
TspRI CASTG 2 cut(s) 316, 607
Tth111I GACNNNGTC 2 cut(s) 218, 227
VpaK11BI GGWCC 1 cut(s) 315
XapI RAATTY 1 cut(s) 738
XceI RCATGY 1 cut(s) 205
XmiI GTMKAC 4 cut(s) 153, 222, 324, 375
XmnI GAANNNNTTC 2 cut(s) 696, 742
XspI CTAG 1 cut(s) 680
ZraI GACGTC 1 cut(s) 373
Zsp2I ATGCAT 1 cut(s) 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.