Rh6BG071700

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000645
Physical Location & Seq
Forward (+)
10842009 .. 10845812
3804 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG071700.1

Sequence Viewer

Length: 792 bp
ATGAACCGACCCCTTCTCCATCCCCTCTTTCTCACATTTTCTTGCTTATTCTTCCTTGCTTCCTCCTCCTCCTCCTCCTCCTCAGGCTACGGTCCCGGCGACACTCCAAAACCCGTTAGGAGGGAAGTATACGGCAATGGGAGAATCTTTGACATCACCCACAGGTACACCCCGGAGATTCCCGTGTATGGTTCAAAGCTTGGGATCGGACATTTCCGGTGGCTCCAACTGAGCATGAAGAATGGTTCACTTACCAACAACTCGGAATTCAAGCTGCCTGTTCACACCGGAACCCATGTTGATGCCCCTGGCCATGTGTTTGATGATTACTATGATGCTGGGTTTGATGTAGATCAGCTTGACTTGGAGGTACTCAATGGCCCTGCCCTTGTTGTTGATGTTCCCAGGGATAGCAACATAACTGCTGAAGTGATGGAGTCCTTAAATATTCCCAAGGGTACTCGCCGAGTACTTTTCAGGACATTAAATACTGATAGGGGTCTTATGCATAAAAAGGAATTTGACTCAAGCTTTGTGGGATTCACGGAGGATGGAGCGAAATGGGTGGTTAAGAACACGGACATCAAACTTGTTGGAACTGATTACTTTTCCATTGCTACCTATGATGAAGCTATTCCGGCACATATTGCTTTTCTAAAGGGAAGGGAAATCATACCTGTGGAAGGCCTAAAGCTTGATGGCATAAAACCAGGAAATTATTCTGTGCATTGCTTACCTCTAAGGTTGCTTGGTGCTGAAGGAGCACCAACAAGATGCATTCTCATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

263

Amino Acids

29.14

Weight (kDa)

6.5

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 50 - 205 1.8e-21 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 129
AclWI GGATC 1 cut(s) 212
AcoI YGGCCR 1 cut(s) 310
AcsI RAATTY 2 cut(s) 266, 518
AcuI CTGAAG 2 cut(s) 447, 777
AfaI GTAC 4 cut(s) 167, 372, 460, 471
AfiI CCNNNNNNNGG 3 cut(s) 120, 188, 683
AgsI TTSAA 2 cut(s) 195, 271
AjnI CCWGG 3 cut(s) 307, 404, 709
AluBI AGCT 6 cut(s) 199, 274, 358, 531, 632, 694
AluI AGCT 6 cut(s) 199, 274, 358, 531, 632, 694
Alw21I GWGCWC 1 cut(s) 766
AlwI GGATC 1 cut(s) 212
AoxI GGCC 3 cut(s) 310, 379, 685
ApeKI GCWGC 1 cut(s) 274
ApoI RAATTY 2 cut(s) 266, 518
ArsI GACNNNNNNTTYG 2 cut(s) 515, 547
Asp700I GAANNNNTTC 2 cut(s) 633, 718
AspS9I GGNCC 2 cut(s) 92, 380
AsuC2I CCSGG 2 cut(s) 96, 173
AsuHPI GGTGA 1 cut(s) 148
AvaII GGWCC 1 cut(s) 92
AxyI CCTNAGG 1 cut(s) 82
BalI TGGCCA 1 cut(s) 312
Bbv12I GWGCWC 1 cut(s) 766
BbvI GCAGC 1 cut(s) 261
BccI CCATC 4 cut(s) 27, 427, 545, 692
BceAI ACGGC 1 cut(s) 148
BciT130I CCWGG 3 cut(s) 309, 406, 711
BcnI CCSGG 2 cut(s) 96, 173
BisI GCNGC 1 cut(s) 275
BlsI GCNGC 1 cut(s) 276
BmcAI AGTACT 1 cut(s) 471
Bme1390I CCNGG 5 cut(s) 96, 173, 309, 406, 711
Bme18I GGWCC 1 cut(s) 92
BmgT120I GGNCC 2 cut(s) 92, 380
BmiI GGNNCC 3 cut(s) 94, 224, 292
BmrFI CCNGG 5 cut(s) 96, 173, 309, 406, 711
BmsI GCATC 3 cut(s) 292, 325, 764
BpuEI CTTGAG 1 cut(s) 511
BpuMI CCSGG 2 cut(s) 96, 173
BsaBI GATNNNNATC 1 cut(s) 351
BsaJI CCNNGG 5 cut(s) 171, 307, 404, 405, 453
BsaWI WCCGGW 2 cut(s) 216, 287
BsaXI ACNNNNNCTCC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 3 cut(s) 120, 188, 683
Bse21I CCTNAGG 1 cut(s) 82
Bse3DI GCAATG 3 cut(s) 142, 612, 727
Bse8I GATNNNNATC 1 cut(s) 351
BseBI CCWGG 3 cut(s) 309, 406, 711
BseDI CCNNGG 5 cut(s) 171, 307, 404, 405, 453
BseGI GGATG 2 cut(s) 19, 556
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 3 cut(s) 120, 188, 683
BseMI GCAATG 3 cut(s) 142, 612, 727
BseMII CTCAG 2 cut(s) 96, 221
BseRI GAGGAG 6 cut(s) 55, 58, 61, 64, 67, 70
BseXI GCAGC 1 cut(s) 261
BseYI CCCAGC 1 cut(s) 338
BshFI GGCC 3 cut(s) 312, 381, 687
BsiHKAI GWGCWC 1 cut(s) 766
BsiSI CCGG 5 cut(s) 96, 173, 217, 288, 638
BslFI GGGAC 1 cut(s) 78
BslI CCNNNNNNNGG 3 cut(s) 120, 188, 683
BsmFI GGGAC 1 cut(s) 78
BsmI GAATGC 1 cut(s) 777
BsnI GGCC 3 cut(s) 312, 381, 687
Bsp1286I GDGCHC 1 cut(s) 766
Bsp143I GATC 2 cut(s) 204, 352
BspANI GGCC 3 cut(s) 312, 381, 687
BspCNI CTCAG 2 cut(s) 95, 222
BspLI GGNNCC 3 cut(s) 94, 224, 292
BspPI GGATC 1 cut(s) 212
BsrDI GCAATG 3 cut(s) 142, 612, 727
BssECI CCNNGG 5 cut(s) 171, 307, 404, 405, 453
BssMI GATC 2 cut(s) 204, 352
BssNAI GTATAC 1 cut(s) 130
BssT1I CCWWGG 1 cut(s) 453
Bst1107I GTATAC 1 cut(s) 130
Bst2UI CCWGG 3 cut(s) 309, 406, 711
Bst4CI ACNGT 1 cut(s) 92
BstAPI GCANNNNNTGC 1 cut(s) 647
BstDEI CTNAG 3 cut(s) 82, 230, 740
BstENI CCTNNNNNAGG 1 cut(s) 681
BstF5I GGATG 2 cut(s) 19, 556
BstKTI GATC 2 cut(s) 207, 355
BstMBI GATC 2 cut(s) 204, 352
BstMWI GCNNNNNNNGC 3 cut(s) 638, 647, 761
BstNI CCWGG 3 cut(s) 309, 406, 711
BstSCI CCNGG 5 cut(s) 94, 171, 307, 404, 709
BstV1I GCAGC 1 cut(s) 261
BstZ17I GTATAC 1 cut(s) 130
Bsu36I CCTNAGG 1 cut(s) 82
BsuRI GGCC 3 cut(s) 312, 381, 687
BtsCI GGATG 2 cut(s) 19, 556
Cfr13I GGNCC 2 cut(s) 92, 380
Csp6I GTAC 4 cut(s) 166, 371, 459, 470
CspCI CAANNNNNGTGG 2 cut(s) 516, 551
CviAII CATG 3 cut(s) 235, 296, 314
CviQI GTAC 4 cut(s) 166, 371, 459, 470
DdeI CTNAG 3 cut(s) 82, 230, 740
DpnI GATC 2 cut(s) 206, 354
DpnII GATC 2 cut(s) 204, 352
EaeI YGGCCR 1 cut(s) 310
Eco130I CCWWGG 1 cut(s) 453
Eco147I AGGCCT 1 cut(s) 687
Eco47I GGWCC 1 cut(s) 92
Eco57I CTGAAG 2 cut(s) 447, 777
Eco81I CCTNAGG 1 cut(s) 82
EcoNI CCTNNNNNAGG 1 cut(s) 681
EcoRI GAATTC 1 cut(s) 266
EcoRII CCWGG 3 cut(s) 307, 404, 709
EcoT14I CCWWGG 1 cut(s) 453
EcoT22I ATGCAT 2 cut(s) 510, 779
ErhI CCWWGG 1 cut(s) 453
FaeI CATG 3 cut(s) 238, 299, 317
FaqI GGGAC 1 cut(s) 78
FatI CATG 3 cut(s) 234, 295, 313
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 1 cut(s) 275
FokI GGATG 2 cut(s) 6, 563
Fsp4HI GCNGC 1 cut(s) 275
GluI GCNGC 1 cut(s) 275
GsaI CCCAGC 1 cut(s) 342
HaeIII GGCC 3 cut(s) 312, 381, 687
HapII CCGG 5 cut(s) 96, 173, 217, 288, 638
Hin1II CATG 3 cut(s) 238, 299, 317
HindIII AAGCTT 3 cut(s) 197, 529, 692
HinfI GANTC 5 cut(s) 144, 178, 437, 524, 540
HpaII CCGG 5 cut(s) 96, 173, 217, 288, 638
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 4 cut(s) 130, 168, 248, 283
Hpy188I TCNGA 2 cut(s) 209, 265
Hpy188III TCNNGA 1 cut(s) 478
Hpy8I GTNNAC 4 cut(s) 130, 168, 248, 283
HpyAV CCTTC 4 cut(s) 23, 657, 677, 752
HpyCH4III ACNGT 1 cut(s) 92
HpyCH4V TGCA 3 cut(s) 508, 727, 777
HpyF10VI GCNNNNNNNGC 3 cut(s) 638, 647, 761
HpyF3I CTNAG 3 cut(s) 82, 230, 740
Hsp92II CATG 3 cut(s) 238, 299, 317
Kzo9I GATC 2 cut(s) 204, 352
LmnI GCTCC 3 cut(s) 228, 554, 761
Lsp1109I GCAGC 1 cut(s) 261
LweI GCATC 3 cut(s) 292, 325, 764
MalI GATC 2 cut(s) 206, 354
MboI GATC 2 cut(s) 204, 352
MboII GAAGA 2 cut(s) 43, 250
MhlI GDGCHC 1 cut(s) 766
MlsI TGGCCA 1 cut(s) 312
MluCI AATT 3 cut(s) 266, 518, 715
MluNI TGGCCA 1 cut(s) 312
MlyI GAGTC 2 cut(s) 446, 518
MmeI TCCRAC 2 cut(s) 250, 574
Mox20I TGGCCA 1 cut(s) 312
Mph1103I ATGCAT 2 cut(s) 510, 779
MroXI GAANNNNTTC 2 cut(s) 633, 718
MscI TGGCCA 1 cut(s) 312
MseI TTAA 3 cut(s) 443, 485, 570
MslI CAYNNNNRTG 2 cut(s) 300, 677
Msp20I TGGCCA 1 cut(s) 312
MspI CCGG 5 cut(s) 96, 173, 217, 288, 638
MspR9I CCNGG 5 cut(s) 96, 173, 309, 406, 711
Mva1269I GAATGC 1 cut(s) 777
MvaI CCWGG 3 cut(s) 309, 406, 711
MwoI GCNNNNNNNGC 3 cut(s) 638, 647, 761
NciI CCSGG 2 cut(s) 96, 173
NdeII GATC 2 cut(s) 204, 352
NlaIII CATG 3 cut(s) 238, 299, 317
NlaIV GGNNCC 3 cut(s) 94, 224, 292
NmeAIII GCCGAG 1 cut(s) 491
NsiI ATGCAT 2 cut(s) 510, 779
PasI CCCWGGG 1 cut(s) 405
PceI AGGCCT 1 cut(s) 687
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 1 cut(s) 777
PdmI GAANNNNTTC 2 cut(s) 633, 718
PfeI GAWTC 3 cut(s) 144, 178, 540
PkrI GCNGC 1 cut(s) 276
PleI GAGTC 2 cut(s) 445, 518
PpsI GAGTC 2 cut(s) 445, 518
Psp6I CCWGG 3 cut(s) 307, 404, 709
PspFI CCCAGC 1 cut(s) 338
PspGI CCWGG 3 cut(s) 307, 404, 709
PspN4I GGNNCC 3 cut(s) 94, 224, 292
PspPI GGNCC 2 cut(s) 92, 380
RsaI GTAC 4 cut(s) 167, 372, 460, 471
RsaNI GTAC 4 cut(s) 166, 371, 459, 470
RseI CAYNNNNRTG 2 cut(s) 300, 677
SaqAI TTAA 3 cut(s) 443, 485, 570
SatI GCNGC 1 cut(s) 275
Sau3AI GATC 2 cut(s) 204, 352
Sau96I GGNCC 2 cut(s) 92, 380
ScaI AGTACT 1 cut(s) 471
SchI GAGTC 2 cut(s) 446, 518
ScrFI CCNGG 5 cut(s) 96, 173, 309, 406, 711
SduI GDGCHC 1 cut(s) 766
SfaNI GCATC 3 cut(s) 292, 325, 764
SinI GGWCC 1 cut(s) 92
SmiMI CAYNNNNRTG 2 cut(s) 300, 677
SmlI CTYRAG 1 cut(s) 526
SmoI CTYRAG 1 cut(s) 526
Sse9I AATT 3 cut(s) 266, 518, 715
SseBI AGGCCT 1 cut(s) 687
SspI AATATT 1 cut(s) 448
StuI AGGCCT 1 cut(s) 687
StyD4I CCNGG 5 cut(s) 94, 171, 307, 404, 709
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 1 cut(s) 92
TasI AATT 3 cut(s) 266, 518, 715
TatI WGTACW 1 cut(s) 469
TfiI GAWTC 3 cut(s) 144, 178, 540
Tru1I TTAA 3 cut(s) 443, 485, 570
Tru9I TTAA 3 cut(s) 443, 485, 570
TseI GCWGC 1 cut(s) 274
TspDTI ATGAA 3 cut(s) 17, 251, 642
TspGWI ACGGA 2 cut(s) 560, 593
VpaK11BI GGWCC 1 cut(s) 92
XagI CCTNNNNNAGG 1 cut(s) 681
XapI RAATTY 2 cut(s) 266, 518
XmiI GTMKAC 1 cut(s) 129
XmnI GAANNNNTTC 2 cut(s) 633, 718
ZrmI AGTACT 1 cut(s) 471
Zsp2I ATGCAT 2 cut(s) 510, 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.