RLG00000014822

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000645
Physical Location & Seq
Reverse (-)
58032994 .. 58050413
17420 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014822

Sequence Viewer

Length: 831 bp
ATGAAGACCCTATTCTTTCTCCTCCTACTCTGTTCCCTTTCGCTCACACTCCCCACTCTCTCCCACGCCACCACAGCCTACCCTTCCGTTCCCGGCACCGCCTCCGCCGACTGTTCTTTATCCGATGGCCACACCGCCCCTCTAATTCCCCCTCGCCACGAAGTTTATGGAAATGGCCGAATCTTCGACATCACCCACCGTATCACCACGGCGATGCCGTCGTATTTCTCCCCGGACGGCGTGCTGGGCCAGTTCCTGTGGCTCCCCCACAGCATGAAGAACGGCTCTGTCGCAAACATGTCAGTTTTCAAGCTGGCTACTCACACCGGCACGCATGTTGATTCACCCGGACACTTCTTCGATCACTACCTCGATGCTGGCTTCGACGTCGATACACTTGACTTGGAGGTGCTTAATGGTCCAGCATTGTTAGTTGATGTTCCGAGGGACAAGAACATAACTGCTGAAGTGATGAAGTCCTTAAATATTCCAAAGGGAGTACGTCGTGTGCTCTTCAGAACATTAAATACTGACAGACGGCTTATGTTCAAAAGTCAGTTTGACGCAAGCTATGTGGGATTCATGAAGGATGGAGCCAAGTGGTTGGTGGAGAACACAGACATCAAACTTGTTGGAATTGATTACTTATCTGTTGCTGCATTTGATGATTTGATTACATCCCACCTTGTTTTTCTAGAAAGCAGGGAAATCATCCTCGTGGAAGCCCTAAAACTTGATGACATCCAACCAGGAATATACTCTGTCCATTGCTTACCTTTGAGGCTGACAGGTGCAGAAGGATCACCAATCAGATGCATTCTTATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

277

Amino Acids

30.47

Weight (kDa)

6.1

Isoelectric Point (pI)

30.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 62 - 218 1.1e-20 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 390
AccB1I GGYRCC 1 cut(s) 95
AciI CCGC 3 cut(s) 99, 105, 135
AclWI GGATC 1 cut(s) 808
AcoI YGGCCR 2 cut(s) 127, 175
AcuI CTGAAG 2 cut(s) 486, 499
AcyI GRCGYC 1 cut(s) 387
AfaI GTAC 1 cut(s) 501
AflIII ACRYGT 1 cut(s) 297
AgsI TTSAA 2 cut(s) 310, 550
AjnI CCWGG 1 cut(s) 748
AluBI AGCT 2 cut(s) 313, 570
AluI AGCT 2 cut(s) 313, 570
Alw21I GWGCWC 1 cut(s) 513
AlwI GGATC 1 cut(s) 808
AlwNI CAGNNNCTG 1 cut(s) 256
AoxI GGCC 3 cut(s) 127, 175, 247
ApeKI GCWGC 1 cut(s) 656
AspS9I GGNCC 2 cut(s) 247, 419
AsuC2I CCSGG 3 cut(s) 93, 233, 348
AsuHPI GGTGA 4 cut(s) 184, 196, 336, 795
AvaII GGWCC 1 cut(s) 419
BalI TGGCCA 1 cut(s) 129
BanI GGYRCC 1 cut(s) 95
BauI CACGAG 1 cut(s) 716
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 513
BbvI GCAGC 1 cut(s) 643
BccI CCATC 2 cut(s) 119, 584
BceAI ACGGC 5 cut(s) 202, 225, 253, 298, 554
BciT130I CCWGG 1 cut(s) 750
BcnI CCSGG 3 cut(s) 93, 233, 348
BfaI CTAG 1 cut(s) 695
BisI GCNGC 1 cut(s) 657
BlsI GCNGC 1 cut(s) 658
Bme1390I CCNGG 4 cut(s) 93, 233, 348, 750
Bme18I GGWCC 1 cut(s) 419
BmgT120I GGNCC 2 cut(s) 247, 419
BmiI GGNNCC 3 cut(s) 97, 263, 595
BmrFI CCNGG 4 cut(s) 93, 233, 348, 750
BmsI GCATC 3 cut(s) 204, 364, 803
BpiI GAAGAC 1 cut(s) 11
BpuMI CCSGG 3 cut(s) 93, 233, 348
BsaHI GRCGYC 1 cut(s) 387
BsaJI CCNNGG 3 cut(s) 207, 231, 443
Bse118I RCCGGY 1 cut(s) 326
Bse1I ACTGG 1 cut(s) 250
Bse3DI GCAATG 1 cut(s) 766
BseBI CCWGG 1 cut(s) 750
BseDI CCNNGG 3 cut(s) 207, 231, 443
BseGI GGATG 4 cut(s) 595, 677, 711, 741
BseMI GCAATG 1 cut(s) 766
BseNI ACTGG 1 cut(s) 250
BseRI GAGGAG 1 cut(s) 11
BseXI GCAGC 1 cut(s) 643
BseYI CCCAGC 1 cut(s) 244
BsgI GTGCAG 1 cut(s) 813
BshFI GGCC 3 cut(s) 129, 177, 249
BshNI GGYRCC 1 cut(s) 95
BsiHKAI GWGCWC 1 cut(s) 513
BsiSI CCGG 4 cut(s) 93, 233, 327, 348
BslFI GGGAC 1 cut(s) 461
BsmFI GGGAC 1 cut(s) 461
BsmI GAATGC 1 cut(s) 816
BsnI GGCC 3 cut(s) 129, 177, 249
Bsp1286I GDGCHC 1 cut(s) 513
Bsp143I GATC 2 cut(s) 361, 800
BspACI CCGC 3 cut(s) 99, 105, 135
BspANI GGCC 3 cut(s) 129, 177, 249
BspHI TCATGA 1 cut(s) 582
BspLI GGNNCC 3 cut(s) 97, 263, 595
BspPI GGATC 1 cut(s) 808
BspQI GCTCTTC 1 cut(s) 518
BspT107I GGYRCC 1 cut(s) 95
BsrDI GCAATG 1 cut(s) 766
BsrFI RCCGGY 1 cut(s) 326
BsrI ACTGG 1 cut(s) 250
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 3 cut(s) 207, 231, 443
BssMI GATC 2 cut(s) 361, 800
BssNI GRCGYC 1 cut(s) 387
BssSI CACGAG 1 cut(s) 716
Bst2BI CACGAG 1 cut(s) 716
Bst2UI CCWGG 1 cut(s) 750
Bst4CI ACNGT 2 cut(s) 113, 200
Bst6I CTCTTC 1 cut(s) 518
BstACI GRCGYC 1 cut(s) 387
BstC8I GCNNGC 5 cut(s) 242, 315, 332, 379, 568
BstDSI CCRYGG 1 cut(s) 207
BstF5I GGATG 4 cut(s) 595, 677, 711, 741
BstKTI GATC 2 cut(s) 364, 803
BstMBI GATC 2 cut(s) 361, 800
BstMWI GCNNNNNNNGC 2 cut(s) 74, 246
BstNI CCWGG 1 cut(s) 750
BstNSI RCATGY 2 cut(s) 301, 338
BstSCI CCNGG 4 cut(s) 91, 231, 346, 748
BstV1I GCAGC 1 cut(s) 643
BstV2I GAAGAC 1 cut(s) 11
BstXI CCANNNNNNTGG 1 cut(s) 604
BsuRI GGCC 3 cut(s) 129, 177, 249
BtgI CCRYGG 1 cut(s) 207
BtgZI GCGATG 1 cut(s) 227
BtsCI GGATG 4 cut(s) 595, 677, 711, 741
Cac8I GCNNGC 5 cut(s) 242, 315, 332, 379, 568
CaiI CAGNNNCTG 1 cut(s) 256
CciI TCATGA 1 cut(s) 582
Cfr10I RCCGGY 1 cut(s) 326
Cfr13I GGNCC 2 cut(s) 247, 419
CseI GACGC 1 cut(s) 572
Csp6I GTAC 1 cut(s) 500
CspCI CAANNNNNGTGG 2 cut(s) 555, 590
CviAII CATG 4 cut(s) 274, 298, 335, 583
CviQI GTAC 1 cut(s) 500
DpnI GATC 2 cut(s) 363, 802
DpnII GATC 2 cut(s) 361, 800
EaeI YGGCCR 2 cut(s) 127, 175
Eam1104I CTCTTC 1 cut(s) 518
EarI CTCTTC 1 cut(s) 518
EciI GGCGGA 1 cut(s) 94
Eco47I GGWCC 1 cut(s) 419
Eco57I CTGAAG 2 cut(s) 486, 499
EcoRII CCWGG 1 cut(s) 748
EcoT22I ATGCAT 1 cut(s) 818
FaeI CATG 4 cut(s) 277, 301, 338, 586
FaiI YATR 9 cut(s) 168, 275, 299, 336, 458, 545, 573, 584, 757
FaqI GGGAC 1 cut(s) 461
FatI CATG 4 cut(s) 273, 297, 334, 582
Fnu4HI GCNGC 1 cut(s) 657
FokI GGATG 4 cut(s) 602, 664, 698, 728
Fsp4HI GCNGC 1 cut(s) 657
FspBI CTAG 1 cut(s) 695
GluI GCNGC 1 cut(s) 657
GsaI CCCAGC 1 cut(s) 248
HaeIII GGCC 3 cut(s) 129, 177, 249
HapII CCGG 4 cut(s) 93, 233, 327, 348
HgaI GACGC 1 cut(s) 572
Hin1I GRCGYC 1 cut(s) 387
Hin1II CATG 4 cut(s) 277, 301, 338, 586
HinfI GANTC 3 cut(s) 180, 341, 579
HpaII CCGG 4 cut(s) 93, 233, 327, 348
HphI GGTGA 4 cut(s) 184, 196, 336, 795
Hpy188I TCNGA 4 cut(s) 124, 444, 518, 812
Hpy188III TCNNGA 2 cut(s) 583, 695
Hpy99I CGWCG 4 cut(s) 223, 389, 392, 507
HpyAV CCTTC 3 cut(s) 93, 580, 791
HpyCH4III ACNGT 2 cut(s) 113, 200
HpyCH4IV ACGT 2 cut(s) 387, 502
HpyCH4V TGCA 3 cut(s) 659, 794, 816
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 246
HpySE526I ACGT 2 cut(s) 387, 502
Hsp92I GRCGYC 1 cut(s) 387
Hsp92II CATG 4 cut(s) 277, 301, 338, 586
Kzo9I GATC 2 cut(s) 361, 800
LguI GCTCTTC 1 cut(s) 518
LmnI GCTCC 2 cut(s) 267, 593
Lsp1109I GCAGC 1 cut(s) 643
LweI GCATC 3 cut(s) 204, 364, 803
MaeI CTAG 1 cut(s) 695
MaeII ACGT 2 cut(s) 387, 502
MalI GATC 2 cut(s) 363, 802
MboI GATC 2 cut(s) 361, 800
MboII GAAGA 5 cut(s) 16, 175, 289, 349, 505
MhlI GDGCHC 1 cut(s) 513
MlsI TGGCCA 1 cut(s) 129
MluCI AATT 2 cut(s) 144, 636
MluNI TGGCCA 1 cut(s) 129
MmeI TCCRAC 2 cut(s) 613, 769
MnlI CCTC 9 cut(s) 32, 112, 150, 162, 380, 400, 438, 725, 774
Mox20I TGGCCA 1 cut(s) 129
Mph1103I ATGCAT 1 cut(s) 818
MscI TGGCCA 1 cut(s) 129
MseI TTAA 3 cut(s) 414, 482, 524
MslI CAYNNNNRTG 2 cut(s) 212, 716
Msp20I TGGCCA 1 cut(s) 129
MspI CCGG 4 cut(s) 93, 233, 327, 348
MspR9I CCNGG 4 cut(s) 93, 233, 348, 750
Mva1269I GAATGC 1 cut(s) 816
MvaI CCWGG 1 cut(s) 750
MwoI GCNNNNNNNGC 2 cut(s) 74, 246
NciI CCSGG 3 cut(s) 93, 233, 348
NdeII GATC 2 cut(s) 361, 800
NlaIII CATG 4 cut(s) 277, 301, 338, 586
NlaIV GGNNCC 3 cut(s) 97, 263, 595
NsiI ATGCAT 1 cut(s) 818
NspI RCATGY 2 cut(s) 301, 338
PagI TCATGA 1 cut(s) 582
PciI ACATGT 1 cut(s) 297
PciSI GCTCTTC 1 cut(s) 518
PcsI WCGNNNNNNNCGW 1 cut(s) 215
PctI GAATGC 1 cut(s) 816
PfeI GAWTC 3 cut(s) 180, 341, 579
PkrI GCNGC 1 cut(s) 658
PscI ACATGT 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 748
PspFI CCCAGC 1 cut(s) 244
PspGI CCWGG 1 cut(s) 748
PspN4I GGNNCC 3 cut(s) 97, 263, 595
PspPI GGNCC 2 cut(s) 247, 419
PsrI GAACNNNNNNTAC 2 cut(s) 511, 543
PstNI CAGNNNCTG 1 cut(s) 256
RsaI GTAC 1 cut(s) 501
RsaNI GTAC 1 cut(s) 500
RseI CAYNNNNRTG 2 cut(s) 212, 716
SapI GCTCTTC 1 cut(s) 518
SaqAI TTAA 3 cut(s) 414, 482, 524
SatI GCNGC 1 cut(s) 657
Sau3AI GATC 2 cut(s) 361, 800
Sau96I GGNCC 2 cut(s) 247, 419
ScrFI CCNGG 4 cut(s) 93, 233, 348, 750
SduI GDGCHC 1 cut(s) 513
SetI ASST 9 cut(s) 315, 372, 390, 411, 505, 572, 687, 778, 793
SfaNI GCATC 3 cut(s) 204, 364, 803
SinI GGWCC 1 cut(s) 419
SmiMI CAYNNNNRTG 2 cut(s) 212, 716
Sse9I AATT 2 cut(s) 144, 636
SsiI CCGC 3 cut(s) 99, 105, 135
SspI AATATT 1 cut(s) 487
SspMI CTAG 1 cut(s) 695
StyD4I CCNGG 4 cut(s) 91, 231, 346, 748
TaaI ACNGT 2 cut(s) 113, 200
TaiI ACGT 2 cut(s) 390, 505
TaqI TCGA 5 cut(s) 186, 360, 372, 384, 390
TasI AATT 2 cut(s) 144, 636
TfiI GAWTC 3 cut(s) 180, 341, 579
Tru1I TTAA 3 cut(s) 414, 482, 524
Tru9I TTAA 3 cut(s) 414, 482, 524
TseI GCWGC 1 cut(s) 656
TspDTI ATGAA 5 cut(s) 17, 290, 488, 571, 599
TspGWI ACGGA 1 cut(s) 76
VpaK11BI GGWCC 1 cut(s) 419
XbaI TCTAGA 1 cut(s) 694
XceI RCATGY 2 cut(s) 301, 338
XcmI CCANNNNNNNNNTGG 2 cut(s) 164, 604
XspI CTAG 1 cut(s) 695
ZraI GACGTC 1 cut(s) 388
Zsp2I ATGCAT 1 cut(s) 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.