Prupe.8G132800_v2.0.a1

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000645
Physical Location & Seq
Reverse (-)
15476771 .. 15479161
2391 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G132800.1

Sequence Viewer

Length: 801 bp
ATGCTCAAAACCATGAACCACCACCAACCCTTTCTTCCTTCCATTTTGCTCACACTTTCTTGCTTCTTCCTCGCTTCCTCCTCCGTCTACAGTTCCGGGGGCGGCGTCAACACCCCAAAACCCGTACGGAGAGAAGTGTACGGCAATGGGAGAATCTTTGACATCACCCACAGGTACACACCGGACCTTCCTTTCTATGGTTCCAAGAATGGGATCGGCCACTTCCTGTGGCTACAGCTCACCATGAAGAATGCTTCTCTCACCAACAACTCTGAGTTCGACCTGCCTGTCCACACTGGAACCCATGTTGATGCGCCTGGCCATGTTTTTGATGACTATTTTGATGCAGGGTTTGATGTTGATCAGCTTGACCTGGATGTCCTCAATGGTCCTGCTCTTGTTGTTGATGTTCCCAGAGATAGCAACATAACTGCTGAAGTTATGAAGTCCCTAAATATTCCCAAGGGTACACGCCGTGTCCTTTTCAAGACACTGAATACTGACAGGCGGCTTATGCATCGAAAGGAATTCGACTCGAGCTTTGTGGGATTCACGGAGGATGGAGCAAAATGGGTTGTAGACAACACAGACATCAAACTTGTTGGAACTGATTACTTTTCCATTGCCACCTATGATGAAGCAATTCCAGCTCATGTTGCCTTTCTCAGAGGCAGGGAAATCATTCCTGTGGAAGGCCTAAAGCTTGACGACATCCTACCCGGAAACTATTCTGTGCATTGCTTGCCTCTAAGGTTGCTTGGTGCTGAAGGAGCACCAACAAGATGCATCCTCATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

267

Amino Acids

29.62

Weight (kDa)

6.04

Isoelectric Point (pI)

38.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 287, 377
Acc36I ACCTGC 1 cut(s) 291
AccI GTMKAC 2 cut(s) 87, 579
AciI CCGC 2 cut(s) 102, 508
AclWI GGATC 1 cut(s) 221
AcoI YGGCCR 2 cut(s) 217, 319
AcsI RAATTY 1 cut(s) 527
AcuI CTGAAG 2 cut(s) 456, 786
AcyI GRCGYC 1 cut(s) 105
AdeI CACNNNGTG 1 cut(s) 476
AfaI GTAC 4 cut(s) 126, 140, 176, 469
AfiI CCNNNNNNNGG 3 cut(s) 197, 210, 692
AgsI TTSAA 1 cut(s) 487
AjnI CCWGG 2 cut(s) 316, 372
AjuI GAANNNNNNNTTGG 2 cut(s) 18, 50
AluBI AGCT 5 cut(s) 238, 367, 540, 650, 703
AluI AGCT 5 cut(s) 238, 367, 540, 650, 703
Alw21I GWGCWC 1 cut(s) 775
AlwI GGATC 1 cut(s) 221
Ama87I CYCGRG 1 cut(s) 535
AoxI GGCC 3 cut(s) 217, 319, 694
ApoI RAATTY 1 cut(s) 527
ArsI GACNNNNNNTTYG 2 cut(s) 524, 556
Asp700I GAANNNNTTC 3 cut(s) 642, 681, 727
AspLEI GCGC 1 cut(s) 316
AspS9I GGNCC 2 cut(s) 184, 389
AsuC2I CCSGG 2 cut(s) 97, 720
AsuHPI GGTGA 3 cut(s) 157, 232, 253
AvaI CYCGRG 1 cut(s) 535
AvaII GGWCC 2 cut(s) 184, 389
BalI TGGCCA 1 cut(s) 321
Bbv12I GWGCWC 1 cut(s) 775
BccI CCATC 1 cut(s) 554
BceAI ACGGC 2 cut(s) 157, 459
BciT130I CCWGG 2 cut(s) 318, 374
BclI TGATCA 1 cut(s) 361
BcnI CCSGG 2 cut(s) 97, 720
BfmI CTRYAG 2 cut(s) 88, 233
BfuAI ACCTGC 1 cut(s) 291
BisI GCNGC 2 cut(s) 103, 509
BlsI GCNGC 2 cut(s) 104, 510
Bme1390I CCNGG 4 cut(s) 97, 318, 374, 720
Bme18I GGWCC 2 cut(s) 184, 389
BmeT110I CYCGRG 1 cut(s) 535
BmgT120I GGNCC 2 cut(s) 184, 389
BmiI GGNNCC 2 cut(s) 202, 301
BmrFI CCNGG 4 cut(s) 97, 318, 374, 720
BmsI GCATC 5 cut(s) 301, 334, 526, 773, 795
BpuMI CCSGG 2 cut(s) 97, 720
BsaBI GATNNNNATC 1 cut(s) 360
BsaHI GRCGYC 1 cut(s) 105
BsaJI CCNNGG 2 cut(s) 96, 462
BsaWI WCCGGW 1 cut(s) 181
Bsc4I CCNNNNNNNGG 3 cut(s) 197, 210, 692
Bse1I ACTGG 1 cut(s) 301
Bse3DI GCAATG 3 cut(s) 151, 621, 736
Bse8I GATNNNNATC 1 cut(s) 360
BseBI CCWGG 2 cut(s) 318, 374
BseDI CCNNGG 2 cut(s) 96, 462
BseGI GGATG 4 cut(s) 382, 565, 711, 786
BseJI GATNNNNATC 1 cut(s) 360
BseLI CCNNNNNNNGG 3 cut(s) 197, 210, 692
BseMI GCAATG 3 cut(s) 151, 621, 736
BseMII CTCAG 2 cut(s) 264, 679
BseNI ACTGG 1 cut(s) 301
BseRI GAGGAG 1 cut(s) 70
BshFI GGCC 3 cut(s) 219, 321, 696
BsiHKAI GWGCWC 1 cut(s) 775
BsiHKCI CYCGRG 1 cut(s) 535
BsiSI CCGG 3 cut(s) 96, 182, 720
BsiWI CGTACG 1 cut(s) 124
BslFI GGGAC 1 cut(s) 433
BslI CCNNNNNNNGG 3 cut(s) 197, 210, 692
BsmFI GGGAC 1 cut(s) 433
BsmI GAATGC 1 cut(s) 256
BsnI GGCC 3 cut(s) 219, 321, 696
BsoBI CYCGRG 1 cut(s) 535
Bsp1286I GDGCHC 1 cut(s) 775
Bsp143I GATC 2 cut(s) 213, 361
BspACI CCGC 2 cut(s) 102, 508
BspANI GGCC 3 cut(s) 219, 321, 696
BspCNI CTCAG 2 cut(s) 265, 678
BspLI GGNNCC 2 cut(s) 202, 301
BspMI ACCTGC 1 cut(s) 291
BspPI GGATC 1 cut(s) 221
BsrDI GCAATG 3 cut(s) 151, 621, 736
BsrI ACTGG 1 cut(s) 301
BssECI CCNNGG 2 cut(s) 96, 462
BssMI GATC 2 cut(s) 213, 361
BssNI GRCGYC 1 cut(s) 105
BssT1I CCWWGG 1 cut(s) 462
Bst2UI CCWGG 2 cut(s) 318, 374
Bst4CI ACNGT 1 cut(s) 92
BstACI GRCGYC 1 cut(s) 105
BstAPI GCANNNNNTGC 1 cut(s) 742
BstC8I GCNNGC 1 cut(s) 743
BstDEI CTNAG 3 cut(s) 273, 665, 749
BstENI CCTNNNNNAGG 1 cut(s) 690
BstF5I GGATG 4 cut(s) 382, 565, 711, 786
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 2 cut(s) 216, 364
BstMBI GATC 2 cut(s) 213, 361
BstMWI GCNNNNNNNGC 5 cut(s) 514, 647, 656, 742, 770
BstNI CCWGG 2 cut(s) 318, 374
BstSCI CCNGG 4 cut(s) 95, 316, 372, 718
BstSFI CTRYAG 2 cut(s) 88, 233
BsuRI GGCC 3 cut(s) 219, 321, 696
BtsCI GGATG 4 cut(s) 382, 565, 711, 786
BtsIMutI CAGTG 2 cut(s) 294, 491
BveI ACCTGC 1 cut(s) 291
Cac8I GCNNGC 1 cut(s) 743
CfoI GCGC 1 cut(s) 316
Cfr13I GGNCC 2 cut(s) 184, 389
CseI GACGC 1 cut(s) 94
Csp6I GTAC 4 cut(s) 125, 139, 175, 468
CviAII CATG 5 cut(s) 13, 244, 305, 323, 653
CviQI GTAC 4 cut(s) 125, 139, 175, 468
DdeI CTNAG 3 cut(s) 273, 665, 749
DpnI GATC 2 cut(s) 215, 363
DpnII GATC 2 cut(s) 213, 361
DraIII CACNNNGTG 1 cut(s) 476
DrdI GACNNNNNNGTC 2 cut(s) 287, 377
DseDI GACNNNNNNGTC 2 cut(s) 287, 377
EaeI YGGCCR 2 cut(s) 217, 319
Eco130I CCWWGG 1 cut(s) 462
Eco147I AGGCCT 1 cut(s) 696
Eco47I GGWCC 2 cut(s) 184, 389
Eco57I CTGAAG 2 cut(s) 456, 786
Eco88I CYCGRG 1 cut(s) 535
EcoNI CCTNNNNNAGG 1 cut(s) 690
EcoRI GAATTC 1 cut(s) 527
EcoRII CCWGG 2 cut(s) 316, 372
EcoT14I CCWWGG 1 cut(s) 462
EcoT22I ATGCAT 2 cut(s) 519, 788
ErhI CCWWGG 1 cut(s) 462
FaeI CATG 5 cut(s) 16, 247, 308, 326, 656
FaqI GGGAC 1 cut(s) 433
FatI CATG 5 cut(s) 12, 243, 304, 322, 652
FbaI TGATCA 1 cut(s) 361
FblI GTMKAC 2 cut(s) 87, 579
Fnu4HI GCNGC 2 cut(s) 103, 509
FokI GGATG 4 cut(s) 389, 572, 698, 773
Fsp4HI GCNGC 2 cut(s) 103, 509
GlaI GCGC 1 cut(s) 315
GluI GCNGC 2 cut(s) 103, 509
HaeIII GGCC 3 cut(s) 219, 321, 696
HapII CCGG 3 cut(s) 96, 182, 720
HgaI GACGC 1 cut(s) 94
HhaI GCGC 1 cut(s) 316
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 5 cut(s) 16, 247, 308, 326, 656
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HindIII AAGCTT 1 cut(s) 701
HinfI GANTC 3 cut(s) 153, 533, 549
HpaII CCGG 3 cut(s) 96, 182, 720
HphI GGTGA 3 cut(s) 157, 232, 253
Hpy166II GTNNAC 7 cut(s) 88, 109, 139, 177, 292, 470, 580
Hpy188I TCNGA 2 cut(s) 274, 668
Hpy188III TCNNGA 1 cut(s) 487
Hpy8I GTNNAC 7 cut(s) 88, 109, 139, 177, 292, 470, 580
HpyAV CCTTC 4 cut(s) 48, 197, 686, 761
HpyCH4III ACNGT 1 cut(s) 92
HpyCH4V TGCA 4 cut(s) 347, 517, 736, 786
HpyF10VI GCNNNNNNNGC 5 cut(s) 514, 647, 656, 742, 770
HpyF3I CTNAG 3 cut(s) 273, 665, 749
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 5 cut(s) 16, 247, 308, 326, 656
HspAI GCGC 1 cut(s) 314
Ksp22I TGATCA 1 cut(s) 361
Kzo9I GATC 2 cut(s) 213, 361
LmnI GCTCC 2 cut(s) 563, 770
LweI GCATC 5 cut(s) 301, 334, 526, 773, 795
MalI GATC 2 cut(s) 215, 363
MboI GATC 2 cut(s) 213, 361
MboII GAAGA 3 cut(s) 26, 58, 259
MhlI GDGCHC 1 cut(s) 775
MlsI TGGCCA 1 cut(s) 321
MluCI AATT 2 cut(s) 527, 642
MluNI TGGCCA 1 cut(s) 321
MlyI GAGTC 1 cut(s) 527
MmeI TCCRAC 1 cut(s) 583
MnlI CCTC 8 cut(s) 80, 88, 91, 392, 550, 662, 756, 800
Mox20I TGGCCA 1 cut(s) 321
Mph1103I ATGCAT 2 cut(s) 519, 788
MroXI GAANNNNTTC 3 cut(s) 642, 681, 727
MscI TGGCCA 1 cut(s) 321
MslI CAYNNNNRTG 2 cut(s) 309, 686
Msp20I TGGCCA 1 cut(s) 321
MspI CCGG 3 cut(s) 96, 182, 720
MspR9I CCNGG 4 cut(s) 97, 318, 374, 720
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 2 cut(s) 318, 374
MwoI GCNNNNNNNGC 5 cut(s) 514, 647, 656, 742, 770
NciI CCSGG 2 cut(s) 97, 720
NdeII GATC 2 cut(s) 213, 361
NlaIII CATG 5 cut(s) 16, 247, 308, 326, 656
NlaIV GGNNCC 2 cut(s) 202, 301
NsiI ATGCAT 2 cut(s) 519, 788
PaeR7I CTCGAG 1 cut(s) 535
PceI AGGCCT 1 cut(s) 696
PctI GAATGC 1 cut(s) 256
PdmI GAANNNNTTC 3 cut(s) 642, 681, 727
PfeI GAWTC 2 cut(s) 153, 549
Pfl23II CGTACG 1 cut(s) 124
PkrI GCNGC 2 cut(s) 104, 510
PleI GAGTC 1 cut(s) 527
PpsI GAGTC 1 cut(s) 527
Psp6I CCWGG 2 cut(s) 316, 372
PspGI CCWGG 2 cut(s) 316, 372
PspLI CGTACG 1 cut(s) 124
PspN4I GGNNCC 2 cut(s) 202, 301
PspPI GGNCC 2 cut(s) 184, 389
PspXI VCTCGAGB 1 cut(s) 535
RsaI GTAC 4 cut(s) 126, 140, 176, 469
RsaNI GTAC 4 cut(s) 125, 139, 175, 468
RseI CAYNNNNRTG 2 cut(s) 309, 686
SatI GCNGC 2 cut(s) 103, 509
Sau3AI GATC 2 cut(s) 213, 361
Sau96I GGNCC 2 cut(s) 184, 389
SchI GAGTC 1 cut(s) 527
ScrFI CCNGG 4 cut(s) 97, 318, 374, 720
SduI GDGCHC 1 cut(s) 775
SfaNI GCATC 5 cut(s) 301, 334, 526, 773, 795
SfcI CTRYAG 2 cut(s) 88, 233
Sfr274I CTCGAG 1 cut(s) 535
SinI GGWCC 2 cut(s) 184, 389
SlaI CTCGAG 1 cut(s) 535
SmiMI CAYNNNNRTG 2 cut(s) 309, 686
SmlI CTYRAG 1 cut(s) 535
SmoI CTYRAG 1 cut(s) 535
Sse9I AATT 2 cut(s) 527, 642
SseBI AGGCCT 1 cut(s) 696
SsiI CCGC 2 cut(s) 102, 508
SspI AATATT 1 cut(s) 457
StuI AGGCCT 1 cut(s) 696
StyD4I CCNGG 4 cut(s) 95, 316, 372, 718
StyI CCWWGG 1 cut(s) 462
TaaI ACNGT 1 cut(s) 92
TaqI TCGA 4 cut(s) 279, 520, 531, 536
TasI AATT 2 cut(s) 527, 642
TauI GCSGC 2 cut(s) 105, 511
TfiI GAWTC 2 cut(s) 153, 549
TscAI CASTG 2 cut(s) 301, 498
TspDTI ATGAA 4 cut(s) 29, 260, 458, 651
TspGWI ACGGA 3 cut(s) 73, 142, 569
TspRI CASTG 2 cut(s) 301, 498
VpaK11BI GGWCC 2 cut(s) 184, 389
XagI CCTNNNNNAGG 1 cut(s) 690
XapI RAATTY 1 cut(s) 527
XhoI CTCGAG 1 cut(s) 535
XmiI GTMKAC 2 cut(s) 87, 579
XmnI GAANNNNTTC 3 cut(s) 642, 681, 727
Zsp2I ATGCAT 2 cut(s) 519, 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.