Rw6G006770

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000645
Physical Location & Seq
Reverse (-)
10836093 .. 10842514
6422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G006770.1

Sequence Viewer

Length: 561 bp
ATGCCAATACGATATGTACAAGAATTGTACCTACACATGAGCATCAAAAATGGCTCGGACGCCAACGTTTCAGTGATGAAGTTCAGTGTCCACTCCGGCACCCATGTGGACCCGCCGGGTCATGTCTTCGACCATTACTACGATGCTGGCTTTGATGTTGATGCGCTTGACCTCGAATTCTTAAATGGTCCTGCACTGGTGGTTGATGCTTTAAATATTCCTAAAGGGGTGCGTCGTGTGCTTTTCAGAACCCTAAATACCGACAGGAAGCTTATGTGGAAGAAAGAGTTTGATACAAGCGCTGTAGGGTTTATGGAGGATGGGGCAAAATGGCTGGTGGACAACACAGACATTAAACTTATTGGGCTTGATTACTTAAACGTCGCAGCTTCTGTTGATGTATTGCCAGTTCATTATGCGTTTCTACAAGGCCGGGAGATGGTTCTTGTTGAGGGTTTAAAGCTCGATGGAGTTCCAGCAGGAATATATTCTGTCCATTGTTTGCCTTTAAGGTTGATCGGTTCTGATGGATCACCAGCAAGATGCATCCTCATCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

186

Amino Acids

20.64

Weight (kDa)

5.75

Isoelectric Point (pI)

34.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 25 - 70 2e-06 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 98
AciI CCGC 1 cut(s) 113
AclI AACGTT 1 cut(s) 66
AclWI GGATC 1 cut(s) 538
AcsI RAATTY 1 cut(s) 176
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 2 cut(s) 18, 29
AfeI AGCGCT 1 cut(s) 301
AfiI CCNNNNNNNGG 1 cut(s) 439
AleI CACNNNNGTG 1 cut(s) 104
AluBI AGCT 3 cut(s) 271, 389, 463
AluI AGCT 3 cut(s) 271, 389, 463
AlwI GGATC 1 cut(s) 538
AlwNI CAGNNNCTG 1 cut(s) 392
Aor51HI AGCGCT 1 cut(s) 301
AoxI GGCC 1 cut(s) 430
ApeKI GCWGC 1 cut(s) 386
ApoI RAATTY 1 cut(s) 176
Asp700I GAANNNNTTC 1 cut(s) 487
AspLEI GCGC 2 cut(s) 166, 302
AspS9I GGNCC 2 cut(s) 109, 188
AsuC2I CCSGG 2 cut(s) 117, 434
AsuHPI GGTGA 1 cut(s) 525
AvaII GGWCC 2 cut(s) 109, 188
BanI GGYRCC 1 cut(s) 98
BbsI GAAGAC 1 cut(s) 118
BbvI GCAGC 1 cut(s) 398
BccI CCATC 4 cut(s) 314, 433, 461, 521
BcnI CCSGG 2 cut(s) 117, 434
BfmI CTRYAG 1 cut(s) 303
BfoI RGCGCY 1 cut(s) 303
BisI GCNGC 1 cut(s) 387
BlsI GCNGC 1 cut(s) 388
Bme1390I CCNGG 2 cut(s) 117, 434
Bme18I GGWCC 2 cut(s) 109, 188
BmgT120I GGNCC 2 cut(s) 109, 188
BmiI GGNNCC 2 cut(s) 100, 111
BmrFI CCNGG 2 cut(s) 117, 434
BmsI GCATC 6 cut(s) 51, 133, 151, 196, 533, 555
BpiI GAAGAC 1 cut(s) 118
BpuMI CCSGG 2 cut(s) 117, 434
BsaHI GRCGYC 1 cut(s) 60
Bsc4I CCNNNNNNNGG 1 cut(s) 439
Bse1I ACTGG 2 cut(s) 201, 407
BseGI GGATG 2 cut(s) 325, 546
BseLI CCNNNNNNNGG 1 cut(s) 439
BseNI ACTGG 2 cut(s) 201, 407
BseXI GCAGC 1 cut(s) 398
BsgI GTGCAG 1 cut(s) 177
BshFI GGCC 1 cut(s) 432
BshNI GGYRCC 1 cut(s) 98
BsiSI CCGG 3 cut(s) 96, 116, 433
BslI CCNNNNNNNGG 1 cut(s) 439
BsnI GGCC 1 cut(s) 432
Bsp1407I TGTACA 1 cut(s) 16
Bsp143I GATC 2 cut(s) 516, 530
BspACI CCGC 1 cut(s) 113
BspANI GGCC 1 cut(s) 432
BspLI GGNNCC 2 cut(s) 100, 111
BspPI GGATC 1 cut(s) 538
BspT107I GGYRCC 1 cut(s) 98
BsrGI TGTACA 1 cut(s) 16
BsrI ACTGG 2 cut(s) 201, 407
BssMI GATC 2 cut(s) 516, 530
BssNI GRCGYC 1 cut(s) 60
BstACI GRCGYC 1 cut(s) 60
BstAUI TGTACA 1 cut(s) 16
BstC8I GCNNGC 1 cut(s) 148
BstF5I GGATG 2 cut(s) 325, 546
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 2 cut(s) 166, 302
BstKTI GATC 2 cut(s) 519, 533
BstMBI GATC 2 cut(s) 516, 530
BstMWI GCNNNNNNNGC 1 cut(s) 238
BstSCI CCNGG 2 cut(s) 115, 432
BstSFI CTRYAG 1 cut(s) 303
BstV1I GCAGC 1 cut(s) 398
BstV2I GAAGAC 1 cut(s) 118
BsuRI GGCC 1 cut(s) 432
BtsCI GGATG 2 cut(s) 325, 546
BtsIMutI CAGTG 3 cut(s) 78, 91, 194
Cac8I GCNNGC 1 cut(s) 148
CaiI CAGNNNCTG 1 cut(s) 392
CfoI GCGC 2 cut(s) 166, 302
Cfr13I GGNCC 2 cut(s) 109, 188
CseI GACGC 2 cut(s) 68, 221
Csp6I GTAC 2 cut(s) 17, 28
CviAII CATG 3 cut(s) 37, 104, 122
CviJI RGCY 8 cut(s) 54, 150, 271, 334, 367, 389, 432, 463
CviKI_1 RGCY 8 cut(s) 54, 150, 271, 334, 367, 389, 432, 463
CviQI GTAC 2 cut(s) 17, 28
DpnI GATC 2 cut(s) 518, 532
DpnII GATC 2 cut(s) 516, 530
DraI TTTAAA 2 cut(s) 213, 459
Eco47I GGWCC 2 cut(s) 109, 188
Eco47III AGCGCT 1 cut(s) 301
EcoRI GAATTC 1 cut(s) 176
EcoT22I ATGCAT 1 cut(s) 548
FaeI CATG 3 cut(s) 40, 107, 125
FaiI YATR 8 cut(s) 15, 38, 105, 123, 275, 314, 417, 487
FatI CATG 3 cut(s) 36, 103, 121
FauI CCCGC 1 cut(s) 120
Fnu4HI GCNGC 1 cut(s) 387
FokI GGATG 2 cut(s) 332, 533
Fsp4HI GCNGC 1 cut(s) 387
GlaI GCGC 2 cut(s) 165, 301
GluI GCNGC 1 cut(s) 387
HaeII RGCGCY 1 cut(s) 303
HaeIII GGCC 1 cut(s) 432
HapII CCGG 3 cut(s) 96, 116, 433
HgaI GACGC 2 cut(s) 68, 221
HhaI GCGC 2 cut(s) 166, 302
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 3 cut(s) 40, 107, 125
Hin6I GCGC 2 cut(s) 164, 300
HinP1I GCGC 2 cut(s) 164, 300
HindIII AAGCTT 1 cut(s) 269
HpaII CCGG 3 cut(s) 96, 116, 433
HphI GGTGA 1 cut(s) 525
Hpy166II GTNNAC 3 cut(s) 91, 109, 340
Hpy188I TCNGA 3 cut(s) 58, 248, 526
Hpy8I GTNNAC 3 cut(s) 91, 109, 340
Hpy99I CGWCG 2 cut(s) 237, 386
HpyCH4IV ACGT 2 cut(s) 66, 381
HpyCH4V TGCA 2 cut(s) 194, 546
HpyF10VI GCNNNNNNNGC 1 cut(s) 238
HpySE526I ACGT 2 cut(s) 66, 381
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 3 cut(s) 40, 107, 125
HspAI GCGC 2 cut(s) 164, 300
Kzo9I GATC 2 cut(s) 516, 530
Lsp1109I GCAGC 1 cut(s) 398
LweI GCATC 6 cut(s) 51, 133, 151, 196, 533, 555
MaeII ACGT 2 cut(s) 66, 381
MalI GATC 2 cut(s) 518, 532
MboI GATC 2 cut(s) 516, 530
MboII GAAGA 2 cut(s) 118, 292
MluCI AATT 2 cut(s) 23, 176
MnlI CCTC 4 cut(s) 182, 310, 445, 560
Mph1103I ATGCAT 1 cut(s) 548
MroXI GAANNNNTTC 1 cut(s) 487
MseI TTAA 6 cut(s) 182, 212, 354, 377, 458, 509
MslI CAYNNNNRTG 1 cut(s) 104
MspI CCGG 3 cut(s) 96, 116, 433
MspR9I CCNGG 2 cut(s) 117, 434
MwoI GCNNNNNNNGC 1 cut(s) 238
NciI CCSGG 2 cut(s) 117, 434
NdeII GATC 2 cut(s) 516, 530
NlaIII CATG 3 cut(s) 40, 107, 125
NlaIV GGNNCC 2 cut(s) 100, 111
NsiI ATGCAT 1 cut(s) 548
OliI CACNNNNGTG 1 cut(s) 104
PdmI GAANNNNTTC 1 cut(s) 487
PkrI GCNGC 1 cut(s) 388
Psp1406I AACGTT 1 cut(s) 66
PspN4I GGNNCC 2 cut(s) 100, 111
PspPI GGNCC 2 cut(s) 109, 188
PsrI GAACNNNNNNTAC 4 cut(s) 241, 273, 393, 425
PstNI CAGNNNCTG 1 cut(s) 392
RsaI GTAC 2 cut(s) 18, 29
RsaNI GTAC 2 cut(s) 17, 28
RseI CAYNNNNRTG 1 cut(s) 104
SaqAI TTAA 6 cut(s) 182, 212, 354, 377, 458, 509
SatI GCNGC 1 cut(s) 387
Sau3AI GATC 2 cut(s) 516, 530
Sau96I GGNCC 2 cut(s) 109, 188
ScrFI CCNGG 2 cut(s) 117, 434
SetI ASST 8 cut(s) 33, 69, 174, 273, 384, 391, 465, 515
SfaNI GCATC 6 cut(s) 51, 133, 151, 196, 533, 555
SfcI CTRYAG 1 cut(s) 303
SinI GGWCC 2 cut(s) 109, 188
SmiMI CAYNNNNRTG 1 cut(s) 104
Sse9I AATT 2 cut(s) 23, 176
SsiI CCGC 1 cut(s) 113
SspI AATATT 1 cut(s) 217
StyD4I CCNGG 2 cut(s) 115, 432
TaiI ACGT 2 cut(s) 69, 384
TaqI TCGA 3 cut(s) 129, 174, 465
TasI AATT 2 cut(s) 23, 176
TatI WGTACW 1 cut(s) 16
Tru1I TTAA 6 cut(s) 182, 212, 354, 377, 458, 509
Tru9I TTAA 6 cut(s) 182, 212, 354, 377, 458, 509
TscAI CASTG 3 cut(s) 78, 91, 201
TseI GCWGC 1 cut(s) 386
TspDTI ATGAA 2 cut(s) 92, 401
TspRI CASTG 3 cut(s) 78, 91, 201
VpaK11BI GGWCC 2 cut(s) 109, 188
XapI RAATTY 1 cut(s) 176
XmnI GAANNNNTTC 1 cut(s) 487
Zsp2I ATGCAT 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.