Rh6CG069900

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000645
Physical Location & Seq
Forward (+)
6911379 .. 6915303
3925 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG069900.1

Sequence Viewer

Length: 681 bp
ATGAACCGACCCCTTCTCCATCCCCTCTTTCTCACATTTTCTTGCTTATTCTTCCTTGCTTCCTCCTCCTCCTCCTCAGGCTACGGTCCCGGCGACACTCCAAAACCCGTTAGGAGGGAAGTATACGGCAATGGGAGAATCTTTGACATCACCCACAGGTACACCCCGGAGATTCCCGAGTATGGTTCAAAGCTTGGGATCGGACGTTTCCGGTGGCTCCAACTCAGCATGAAGAATGGTTCACTTACCAACAACTCGGAGTTCAAGCTGCCTGTTCACACCGGAACCCATGTTGATGCCCCTGGCCATGTGTTTGATGATTACTATGATGCTGGGTTTGATGTAGATCAGCTTGACTTGGAGGTACTCAATGGCCCTGCCCTTGTTGTTGATGTTCCCAGGGATAGCAACATAACTGCTGAAGTGATGGAGTCCTTAAATATTCCCAAGGGTACTCGCCGCGTACTTTTCAGGACATTAAATACTGATAGGGGTCTTATGCATAAAAAGGAATTTGACTCAAGCTTTGTGGGATTCACGGAGGATGGAGCGAAATGGGTGGTTAAGAACACGGACATCAAATTTGTTGGAACTGATTACTTTTCCATTGCTACCTATGATGAAGCTATTCCGGCACATATTGCTTTTCTAAAGGGAAGGGTAAGAATTCTTATTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

226

Amino Acids

25.39

Weight (kDa)

6.38

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 48 - 203 7.4e-21 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 123
AccII CGCG 1 cut(s) 462
AciI CCGC 1 cut(s) 460
AclWI GGATC 1 cut(s) 206
AcoI YGGCCR 1 cut(s) 304
AcsI RAATTY 3 cut(s) 512, 581, 666
AcuI CTGAAG 1 cut(s) 441
AfaI GTAC 4 cut(s) 161, 366, 454, 465
AfiI CCNNNNNNNGG 2 cut(s) 114, 182
AgsI TTSAA 3 cut(s) 189, 265, 677
AjnI CCWGG 2 cut(s) 301, 398
AluBI AGCT 5 cut(s) 193, 268, 352, 525, 626
AluI AGCT 5 cut(s) 193, 268, 352, 525, 626
AlwI GGATC 1 cut(s) 206
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 2 cut(s) 304, 373
ApeKI GCWGC 1 cut(s) 268
ApoI RAATTY 3 cut(s) 512, 581, 666
ArsI GACNNNNNNTTYG 4 cut(s) 509, 541, 566, 598
Asp700I GAANNNNTTC 1 cut(s) 627
AspS9I GGNCC 2 cut(s) 86, 374
AsuC2I CCSGG 2 cut(s) 90, 167
AsuHPI GGTGA 1 cut(s) 142
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 1 cut(s) 86
AxyI CCTNAGG 1 cut(s) 76
BalI TGGCCA 1 cut(s) 306
BbvI GCAGC 1 cut(s) 255
BccI CCATC 3 cut(s) 27, 421, 539
BceAI ACGGC 1 cut(s) 142
BciT130I CCWGG 2 cut(s) 303, 400
BcnI CCSGG 2 cut(s) 90, 167
BisI GCNGC 2 cut(s) 269, 460
BlsI GCNGC 2 cut(s) 270, 461
Bme1390I CCNGG 4 cut(s) 90, 167, 303, 400
Bme18I GGWCC 1 cut(s) 86
BmeT110I CYCGRG 1 cut(s) 176
BmgT120I GGNCC 2 cut(s) 86, 374
BmiI GGNNCC 3 cut(s) 88, 218, 286
BmrFI CCNGG 4 cut(s) 90, 167, 303, 400
BmsI GCATC 2 cut(s) 286, 319
BpuEI CTTGAG 1 cut(s) 505
BpuMI CCSGG 2 cut(s) 90, 167
BsaBI GATNNNNATC 1 cut(s) 345
BsaJI CCNNGG 5 cut(s) 165, 301, 398, 399, 447
BsaWI WCCGGW 2 cut(s) 210, 281
BsaXI ACNNNNNCTCC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 2 cut(s) 114, 182
Bse21I CCTNAGG 1 cut(s) 76
Bse3DI GCAATG 2 cut(s) 136, 606
Bse8I GATNNNNATC 1 cut(s) 345
BseBI CCWGG 2 cut(s) 303, 400
BseDI CCNNGG 5 cut(s) 165, 301, 398, 399, 447
BseGI GGATG 2 cut(s) 19, 550
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 2 cut(s) 114, 182
BseMI GCAATG 2 cut(s) 136, 606
BseMII CTCAG 2 cut(s) 90, 238
BseRI GAGGAG 4 cut(s) 55, 58, 61, 64
BseXI GCAGC 1 cut(s) 255
BseYI CCCAGC 1 cut(s) 332
Bsh1236I CGCG 1 cut(s) 462
BshFI GGCC 2 cut(s) 306, 375
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 5 cut(s) 90, 167, 211, 282, 632
BslFI GGGAC 1 cut(s) 72
BslI CCNNNNNNNGG 2 cut(s) 114, 182
BsmFI GGGAC 1 cut(s) 72
BsnI GGCC 2 cut(s) 306, 375
BsoBI CYCGRG 1 cut(s) 176
Bsp143I GATC 2 cut(s) 198, 346
BspACI CCGC 1 cut(s) 460
BspANI GGCC 2 cut(s) 306, 375
BspCNI CTCAG 2 cut(s) 89, 237
BspFNI CGCG 1 cut(s) 462
BspLI GGNNCC 3 cut(s) 88, 218, 286
BspPI GGATC 1 cut(s) 206
BsrDI GCAATG 2 cut(s) 136, 606
BssECI CCNNGG 5 cut(s) 165, 301, 398, 399, 447
BssMI GATC 2 cut(s) 198, 346
BssNAI GTATAC 1 cut(s) 124
BssT1I CCWWGG 1 cut(s) 447
Bst1107I GTATAC 1 cut(s) 124
Bst2UI CCWGG 2 cut(s) 303, 400
Bst4CI ACNGT 1 cut(s) 86
BstAPI GCANNNNNTGC 1 cut(s) 641
BstDEI CTNAG 2 cut(s) 76, 224
BstF5I GGATG 2 cut(s) 19, 550
BstFNI CGCG 1 cut(s) 462
BstKTI GATC 2 cut(s) 201, 349
BstMBI GATC 2 cut(s) 198, 346
BstMWI GCNNNNNNNGC 2 cut(s) 632, 641
BstNI CCWGG 2 cut(s) 303, 400
BstSCI CCNGG 4 cut(s) 88, 165, 301, 398
BstUI CGCG 1 cut(s) 462
BstV1I GCAGC 1 cut(s) 255
BstZ17I GTATAC 1 cut(s) 124
Bsu36I CCTNAGG 1 cut(s) 76
BsuRI GGCC 2 cut(s) 306, 375
BtsCI GGATG 2 cut(s) 19, 550
Cfr13I GGNCC 2 cut(s) 86, 374
Csp6I GTAC 4 cut(s) 160, 365, 453, 464
CspCI CAANNNNNGTGG 2 cut(s) 510, 545
CviAII CATG 3 cut(s) 229, 290, 308
CviJI RGCY 9 cut(s) 81, 193, 217, 268, 306, 352, 375, 525, 626
CviKI_1 RGCY 9 cut(s) 81, 193, 217, 268, 306, 352, 375, 525, 626
CviQI GTAC 4 cut(s) 160, 365, 453, 464
DdeI CTNAG 2 cut(s) 76, 224
DpnI GATC 2 cut(s) 200, 348
DpnII GATC 2 cut(s) 198, 346
EaeI YGGCCR 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 447
Eco47I GGWCC 1 cut(s) 86
Eco57I CTGAAG 1 cut(s) 441
Eco81I CCTNAGG 1 cut(s) 76
Eco88I CYCGRG 1 cut(s) 176
EcoRI GAATTC 1 cut(s) 666
EcoRII CCWGG 2 cut(s) 301, 398
EcoT14I CCWWGG 1 cut(s) 447
EcoT22I ATGCAT 1 cut(s) 504
ErhI CCWWGG 1 cut(s) 447
FaeI CATG 3 cut(s) 232, 293, 311
FaqI GGGAC 1 cut(s) 72
FatI CATG 3 cut(s) 228, 289, 307
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 2 cut(s) 269, 460
FokI GGATG 2 cut(s) 6, 557
Fsp4HI GCNGC 2 cut(s) 269, 460
GluI GCNGC 2 cut(s) 269, 460
GsaI CCCAGC 1 cut(s) 336
HaeIII GGCC 2 cut(s) 306, 375
HapII CCGG 5 cut(s) 90, 167, 211, 282, 632
Hin1II CATG 3 cut(s) 232, 293, 311
HindIII AAGCTT 2 cut(s) 191, 523
HinfI GANTC 5 cut(s) 138, 172, 431, 518, 534
HpaII CCGG 5 cut(s) 90, 167, 211, 282, 632
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 4 cut(s) 124, 162, 242, 277
Hpy188I TCNGA 2 cut(s) 203, 259
Hpy188III TCNNGA 2 cut(s) 176, 472
Hpy8I GTNNAC 4 cut(s) 124, 162, 242, 277
HpyAV CCTTC 2 cut(s) 23, 651
HpyCH4III ACNGT 1 cut(s) 86
HpyCH4IV ACGT 1 cut(s) 205
HpyCH4V TGCA 1 cut(s) 502
HpyF10VI GCNNNNNNNGC 2 cut(s) 632, 641
HpyF3I CTNAG 2 cut(s) 76, 224
HpySE526I ACGT 1 cut(s) 205
Hsp92II CATG 3 cut(s) 232, 293, 311
Kzo9I GATC 2 cut(s) 198, 346
LmnI GCTCC 2 cut(s) 222, 548
Lsp1109I GCAGC 1 cut(s) 255
LweI GCATC 2 cut(s) 286, 319
MaeII ACGT 1 cut(s) 205
MalI GATC 2 cut(s) 200, 348
MboI GATC 2 cut(s) 198, 346
MboII GAAGA 2 cut(s) 43, 244
MlsI TGGCCA 1 cut(s) 306
MluCI AATT 3 cut(s) 512, 581, 666
MluNI TGGCCA 1 cut(s) 306
MlyI GAGTC 2 cut(s) 440, 512
MmeI TCCRAC 2 cut(s) 244, 568
MnlI CCTC 9 cut(s) 35, 73, 76, 79, 82, 85, 108, 355, 535
Mox20I TGGCCA 1 cut(s) 306
Mph1103I ATGCAT 1 cut(s) 504
MroXI GAANNNNTTC 1 cut(s) 627
MscI TGGCCA 1 cut(s) 306
MseI TTAA 3 cut(s) 437, 479, 564
MslI CAYNNNNRTG 1 cut(s) 294
Msp20I TGGCCA 1 cut(s) 306
MspI CCGG 5 cut(s) 90, 167, 211, 282, 632
MspR9I CCNGG 4 cut(s) 90, 167, 303, 400
MvaI CCWGG 2 cut(s) 303, 400
MvnI CGCG 1 cut(s) 462
MwoI GCNNNNNNNGC 2 cut(s) 632, 641
NciI CCSGG 2 cut(s) 90, 167
NdeII GATC 2 cut(s) 198, 346
NlaIII CATG 3 cut(s) 232, 293, 311
NlaIV GGNNCC 3 cut(s) 88, 218, 286
NsiI ATGCAT 1 cut(s) 504
PasI CCCWGGG 1 cut(s) 399
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PdmI GAANNNNTTC 1 cut(s) 627
PfeI GAWTC 3 cut(s) 138, 172, 534
PkrI GCNGC 2 cut(s) 270, 461
PleI GAGTC 2 cut(s) 439, 512
PpsI GAGTC 2 cut(s) 439, 512
Psp6I CCWGG 2 cut(s) 301, 398
PspFI CCCAGC 1 cut(s) 332
PspGI CCWGG 2 cut(s) 301, 398
PspN4I GGNNCC 3 cut(s) 88, 218, 286
PspPI GGNCC 2 cut(s) 86, 374
RsaI GTAC 4 cut(s) 161, 366, 454, 465
RsaNI GTAC 4 cut(s) 160, 365, 453, 464
RseI CAYNNNNRTG 1 cut(s) 294
SaqAI TTAA 3 cut(s) 437, 479, 564
SatI GCNGC 2 cut(s) 269, 460
Sau3AI GATC 2 cut(s) 198, 346
Sau96I GGNCC 2 cut(s) 86, 374
SchI GAGTC 2 cut(s) 440, 512
ScrFI CCNGG 4 cut(s) 90, 167, 303, 400
SetI ASST 9 cut(s) 161, 195, 208, 270, 354, 366, 527, 617, 628
SfaNI GCATC 2 cut(s) 286, 319
SinI GGWCC 1 cut(s) 86
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 1 cut(s) 520
SmoI CTYRAG 1 cut(s) 520
Sse9I AATT 3 cut(s) 512, 581, 666
SsiI CCGC 1 cut(s) 460
SspI AATATT 1 cut(s) 442
StyD4I CCNGG 4 cut(s) 88, 165, 301, 398
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 1 cut(s) 86
TaiI ACGT 1 cut(s) 208
TasI AATT 3 cut(s) 512, 581, 666
TauI GCSGC 1 cut(s) 462
TfiI GAWTC 3 cut(s) 138, 172, 534
Tru1I TTAA 3 cut(s) 437, 479, 564
Tru9I TTAA 3 cut(s) 437, 479, 564
TseI GCWGC 1 cut(s) 268
TspDTI ATGAA 3 cut(s) 17, 245, 636
TspGWI ACGGA 2 cut(s) 554, 587
VpaK11BI GGWCC 1 cut(s) 86
XapI RAATTY 3 cut(s) 512, 581, 666
XmiI GTMKAC 1 cut(s) 123
XmnI GAANNNNTTC 1 cut(s) 627
Zsp2I ATGCAT 1 cut(s) 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.