Rroxscaffold_7G00209780

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000645
Physical Location & Seq
Forward (+)
60053655 .. 60055625
1971 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00209780.1

Sequence Viewer

Length: 831 bp
ATGGATCACATTCACCATTTTCTTCCTCTTCTGCTGCTACTACCTTGCACGGCCGTTGCCACCGTCACCTCATCCTCTGACTCAGCTTACCCCACAGTCACCGAGGACTGCTCGGTGGGCTCCGGCGAGGCCGTCCCTGTCCCGGTACGTAGAGAGGTTTACGATAACGGTCGAATATTCGACATCAGCCACAGGTACACCGCTGAGATGCCGAGTTTCGCCTCCGAAACCGGGCGGCTGGGCAGCGACTTCTTGTGGCTGGGATTCAGCATCAAGAACGGATCGCTGGCAAACGCCTCCATGATGAAGTTCTGTGTCCACTCGGGCACTCACGTCGACGCACCTGGTCACGTCTTCGACCATTACTACCATGCTGGATTTGATGTCGATGCTTTAGACCTCGAAGTGTTAAACGGCCCTGCGCTGGTGGTTGATGTTCCCAGGGATAAGAACATAACTGCTGAAGTAATGAAGGCTTTGAACATTCCTAAAGGAGTGCGTCGTGTGCTTTTCAGAACCTTAAATACTGACAGAAAAATTATGTGGAACAAAGCGTACGACACGAGCGCCACAGGATTTACGGAGGATGGGGCAAAATGGTTAGTTCAAAACACAGACATCAAACTCGTTGGAATTGATTACTTAAACGTTGCAGCTTTCGTTGATGTAGTTCCAGTGCATAATACTTTTCTGGAAGGCAGGGAGATAATTCTTGTCGAAGGTTTAAAGCTCGAAGGAGTTCCGGCAGGATTATATTCTTCTGTTCATTGCTTGCCTTTAAGGTTGATTGGTTCCGATGGATCACCAGCAAGATGCATTCTCATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

276

Amino Acids

30.2

Weight (kDa)

5.73

Isoelectric Point (pI)

41.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 60 - 217 2.8e-21 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 336
AciI CCGC 2 cut(s) 201, 235
AclI AACGTT 1 cut(s) 648
AclWI GGATC 3 cut(s) 12, 289, 808
AcoI YGGCCR 1 cut(s) 51
AcuI CTGAAG 1 cut(s) 483
AfaI GTAC 3 cut(s) 147, 197, 557
AfiI CCNNNNNNNGG 3 cut(s) 142, 231, 424
AgsI TTSAA 2 cut(s) 481, 608
AjiI CACGTC 2 cut(s) 334, 352
AjnI CCWGG 2 cut(s) 343, 440
AluBI AGCT 3 cut(s) 86, 656, 730
AluI AGCT 3 cut(s) 86, 656, 730
AlwI GGATC 3 cut(s) 12, 289, 808
Ama87I CYCGRG 1 cut(s) 322
AoxI GGCC 3 cut(s) 51, 129, 415
ApeKI GCWGC 3 cut(s) 34, 243, 653
Asp700I GAANNNNTTC 1 cut(s) 738
AspLEI GCGC 2 cut(s) 424, 569
AspS9I GGNCC 1 cut(s) 416
AsuC2I CCSGG 2 cut(s) 143, 232
AsuHPI GGTGA 4 cut(s) 5, 58, 91, 795
AvaI CYCGRG 1 cut(s) 322
BaeGI GKGCMC 1 cut(s) 329
BanII GRGCYC 1 cut(s) 122
BauI CACGAG 1 cut(s) 562
BbsI GAAGAC 1 cut(s) 346
BbvI GCAGC 3 cut(s) 21, 255, 665
BccI CCATC 2 cut(s) 581, 791
BceAI ACGGC 4 cut(s) 38, 66, 116, 430
BcgI CGANNNNNNTGC 2 cut(s) 316, 350
BciT130I CCWGG 2 cut(s) 345, 442
BcnI CCSGG 2 cut(s) 143, 232
BfoI RGCGCY 1 cut(s) 570
BisI GCNGC 4 cut(s) 35, 236, 244, 654
BlsI GCNGC 4 cut(s) 36, 237, 245, 655
Bme1390I CCNGG 4 cut(s) 143, 232, 345, 442
BmeT110I CYCGRG 1 cut(s) 322
BmgBI CACGTC 2 cut(s) 334, 352
BmgT120I GGNCC 1 cut(s) 416
BmiI GGNNCC 2 cut(s) 121, 793
BmrFI CCNGG 4 cut(s) 143, 232, 345, 442
BmsI GCATC 4 cut(s) 198, 279, 379, 803
BpiI GAAGAC 1 cut(s) 346
BplI GAGNNNNNCTC 2 cut(s) 95, 127
BpuMI CCSGG 2 cut(s) 143, 232
BsaAI YACGTR 1 cut(s) 149
BsaJI CCNNGG 3 cut(s) 102, 440, 441
Bsc4I CCNNNNNNNGG 3 cut(s) 142, 231, 424
Bse1I ACTGG 1 cut(s) 674
Bse3DI GCAATG 1 cut(s) 766
BseBI CCWGG 2 cut(s) 345, 442
BseDI CCNNGG 3 cut(s) 102, 440, 441
BseGI GGATG 2 cut(s) 71, 592
BseLI CCNNNNNNNGG 3 cut(s) 142, 231, 424
BseMI GCAATG 1 cut(s) 766
BseMII CTCAG 2 cut(s) 96, 195
BseNI ACTGG 1 cut(s) 674
BseSI GKGCMC 1 cut(s) 329
BseX3I CGGCCG 1 cut(s) 51
BseXI GCAGC 3 cut(s) 21, 255, 665
BseYI CCCAGC 2 cut(s) 238, 259
Bsh1285I CGRYCG 2 cut(s) 54, 172
BshFI GGCC 3 cut(s) 53, 131, 417
BsiEI CGRYCG 2 cut(s) 54, 172
BsiHKCI CYCGRG 1 cut(s) 322
BsiSI CCGG 4 cut(s) 123, 143, 231, 743
BsiWI CGTACG 1 cut(s) 555
BslFI GGGAC 2 cut(s) 119, 125
BslI CCNNNNNNNGG 3 cut(s) 142, 231, 424
BsmFI GGGAC 2 cut(s) 119, 125
BsmI GAATGC 1 cut(s) 816
BsnI GGCC 3 cut(s) 53, 131, 417
BsoBI CYCGRG 1 cut(s) 322
Bsp1286I GDGCHC 2 cut(s) 122, 329
Bsp143I GATC 3 cut(s) 4, 281, 800
BspACI CCGC 2 cut(s) 201, 235
BspANI GGCC 3 cut(s) 53, 131, 417
BspCNI CTCAG 2 cut(s) 95, 196
BspLI GGNNCC 2 cut(s) 121, 793
BspPI GGATC 3 cut(s) 12, 289, 808
BsrDI GCAATG 1 cut(s) 766
BsrI ACTGG 1 cut(s) 674
BssECI CCNNGG 3 cut(s) 102, 440, 441
BssMI GATC 3 cut(s) 4, 281, 800
BssSI CACGAG 1 cut(s) 562
Bst2BI CACGAG 1 cut(s) 562
Bst2UI CCWGG 2 cut(s) 345, 442
Bst4CI ACNGT 3 cut(s) 64, 97, 170
Bst6I CTCTTC 1 cut(s) 33
BstBAI YACGTR 1 cut(s) 149
BstC8I GCNNGC 2 cut(s) 288, 773
BstDEI CTNAG 2 cut(s) 82, 204
BstF5I GGATG 2 cut(s) 71, 592
BstH2I RGCGCY 1 cut(s) 570
BstHHI GCGC 2 cut(s) 424, 569
BstKTI GATC 3 cut(s) 7, 284, 803
BstMBI GATC 3 cut(s) 4, 281, 800
BstMCI CGRYCG 2 cut(s) 54, 172
BstMWI GCNNNNNNNGC 2 cut(s) 117, 505
BstNI CCWGG 2 cut(s) 345, 442
BstSCI CCNGG 4 cut(s) 141, 230, 343, 440
BstSLI GKGCMC 1 cut(s) 329
BstSNI TACGTA 1 cut(s) 149
BstV1I GCAGC 3 cut(s) 21, 255, 665
BstV2I GAAGAC 1 cut(s) 346
BstZI CGGCCG 1 cut(s) 51
BsuRI GGCC 3 cut(s) 53, 131, 417
BtrI CACGTC 2 cut(s) 334, 352
BtsCI GGATG 2 cut(s) 71, 592
BtsIMutI CAGTG 1 cut(s) 681
Cac8I GCNNGC 2 cut(s) 288, 773
CfoI GCGC 2 cut(s) 424, 569
Cfr13I GGNCC 1 cut(s) 416
CseI GACGC 2 cut(s) 347, 488
CsiI ACCWGGT 1 cut(s) 343
Csp6I GTAC 3 cut(s) 146, 196, 556
CviAII CATG 2 cut(s) 301, 371
CviQI GTAC 3 cut(s) 146, 196, 556
DdeI CTNAG 2 cut(s) 82, 204
DpnI GATC 3 cut(s) 6, 283, 802
DpnII GATC 3 cut(s) 4, 281, 800
DraI TTTAAA 1 cut(s) 726
EaeI YGGCCR 1 cut(s) 51
EagI CGGCCG 1 cut(s) 51
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
EclXI CGGCCG 1 cut(s) 51
Eco105I TACGTA 1 cut(s) 149
Eco24I GRGCYC 1 cut(s) 122
Eco52I CGGCCG 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 483
Eco88I CYCGRG 1 cut(s) 322
EcoRII CCWGG 2 cut(s) 343, 440
EcoT22I ATGCAT 1 cut(s) 818
EcoT38I GRGCYC 1 cut(s) 122
FaeI CATG 2 cut(s) 304, 374
FaiI YATR 6 cut(s) 302, 372, 455, 542, 681, 754
FaqI GGGAC 2 cut(s) 119, 125
FatI CATG 2 cut(s) 300, 370
FblI GTMKAC 1 cut(s) 336
Fnu4HI GCNGC 4 cut(s) 35, 236, 244, 654
FokI GGATG 2 cut(s) 58, 599
FriOI GRGCYC 1 cut(s) 122
Fsp4HI GCNGC 4 cut(s) 35, 236, 244, 654
GlaI GCGC 2 cut(s) 423, 568
GluI GCNGC 4 cut(s) 35, 236, 244, 654
GsaI CCCAGC 2 cut(s) 242, 263
HaeII RGCGCY 1 cut(s) 570
HaeIII GGCC 3 cut(s) 53, 131, 417
HapII CCGG 4 cut(s) 123, 143, 231, 743
HgaI GACGC 2 cut(s) 347, 488
HhaI GCGC 2 cut(s) 424, 569
Hin1II CATG 2 cut(s) 304, 374
Hin6I GCGC 2 cut(s) 422, 567
HinP1I GCGC 2 cut(s) 422, 567
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HinfI GANTC 2 cut(s) 80, 264
HpaII CCGG 4 cut(s) 123, 143, 231, 743
HphI GGTGA 4 cut(s) 5, 58, 91, 795
Hpy166II GTNNAC 4 cut(s) 160, 198, 319, 337
Hpy188I TCNGA 4 cut(s) 79, 226, 515, 796
Hpy188III TCNNGA 2 cut(s) 274, 692
Hpy8I GTNNAC 4 cut(s) 160, 198, 319, 337
Hpy99I CGWCG 3 cut(s) 338, 341, 504
HpyAV CCTTC 4 cut(s) 466, 689, 713, 728
HpyCH4III ACNGT 3 cut(s) 64, 97, 170
HpyCH4IV ACGT 4 cut(s) 148, 333, 351, 648
HpyCH4V TGCA 4 cut(s) 48, 653, 679, 816
HpyF10VI GCNNNNNNNGC 2 cut(s) 117, 505
HpyF3I CTNAG 2 cut(s) 82, 204
HpySE526I ACGT 4 cut(s) 148, 333, 351, 648
Hsp92II CATG 2 cut(s) 304, 374
HspAI GCGC 2 cut(s) 422, 567
Kzo9I GATC 3 cut(s) 4, 281, 800
LmnI GCTCC 1 cut(s) 125
Lsp1109I GCAGC 3 cut(s) 21, 255, 665
LweI GCATC 4 cut(s) 198, 279, 379, 803
MabI ACCWGGT 1 cut(s) 343
MaeII ACGT 4 cut(s) 148, 333, 351, 648
MaeIII GTNAC 3 cut(s) 64, 97, 347
MalI GATC 3 cut(s) 6, 283, 802
MboI GATC 3 cut(s) 4, 281, 800
MboII GAAGA 4 cut(s) 14, 20, 346, 750
MhlI GDGCHC 2 cut(s) 122, 329
MluCI AATT 3 cut(s) 537, 633, 708
MlyI GAGTC 1 cut(s) 74
MmeI TCCRAC 1 cut(s) 610
Mph1103I ATGCAT 1 cut(s) 818
MroXI GAANNNNTTC 1 cut(s) 738
MseI TTAA 5 cut(s) 410, 521, 644, 725, 779
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 4 cut(s) 123, 143, 231, 743
MspR9I CCNGG 4 cut(s) 143, 232, 345, 442
Mva1269I GAATGC 1 cut(s) 816
MvaI CCWGG 2 cut(s) 345, 442
MwoI GCNNNNNNNGC 2 cut(s) 117, 505
NciI CCSGG 2 cut(s) 143, 232
NdeII GATC 3 cut(s) 4, 281, 800
NlaIII CATG 2 cut(s) 304, 374
NlaIV GGNNCC 2 cut(s) 121, 793
NmeAIII GCCGAG 1 cut(s) 237
NmuCI GTSAC 3 cut(s) 64, 97, 347
NsiI ATGCAT 1 cut(s) 818
PasI CCCWGGG 1 cut(s) 441
PctI GAATGC 1 cut(s) 816
PdmI GAANNNNTTC 1 cut(s) 738
PfeI GAWTC 1 cut(s) 264
Pfl23II CGTACG 1 cut(s) 555
PkrI GCNGC 4 cut(s) 36, 237, 245, 655
PleI GAGTC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 74
Ppu21I YACGTR 1 cut(s) 149
Psp1406I AACGTT 1 cut(s) 648
Psp6I CCWGG 2 cut(s) 343, 440
PspFI CCCAGC 2 cut(s) 238, 259
PspGI CCWGG 2 cut(s) 343, 440
PspLI CGTACG 1 cut(s) 555
PspN4I GGNNCC 2 cut(s) 121, 793
PspPI GGNCC 1 cut(s) 416
PsrI GAACNNNNNNTAC 4 cut(s) 508, 539, 540, 571
RsaI GTAC 3 cut(s) 147, 197, 557
RsaNI GTAC 3 cut(s) 146, 196, 556
SalI GTCGAC 1 cut(s) 335
SaqAI TTAA 5 cut(s) 410, 521, 644, 725, 779
SatI GCNGC 4 cut(s) 35, 236, 244, 654
Sau3AI GATC 3 cut(s) 4, 281, 800
Sau96I GGNCC 1 cut(s) 416
SchI GAGTC 1 cut(s) 74
ScrFI CCNGG 4 cut(s) 143, 232, 345, 442
SduI GDGCHC 2 cut(s) 122, 329
SexAI ACCWGGT 1 cut(s) 343
SfaNI GCATC 4 cut(s) 198, 279, 379, 803
SgrDI CGTCGACG 1 cut(s) 335
SnaBI TACGTA 1 cut(s) 149
Sse9I AATT 3 cut(s) 537, 633, 708
SsiI CCGC 2 cut(s) 201, 235
SspI AATATT 1 cut(s) 177
StyD4I CCNGG 4 cut(s) 141, 230, 343, 440
TaaI ACNGT 3 cut(s) 64, 97, 170
TaiI ACGT 4 cut(s) 151, 336, 354, 651
TaqI TCGA 8 cut(s) 172, 180, 336, 357, 387, 402, 717, 732
TasI AATT 3 cut(s) 537, 633, 708
TauI GCSGC 1 cut(s) 238
TfiI GAWTC 1 cut(s) 264
Tru1I TTAA 5 cut(s) 410, 521, 644, 725, 779
Tru9I TTAA 5 cut(s) 410, 521, 644, 725, 779
TscAI CASTG 1 cut(s) 681
TseFI GTSAC 3 cut(s) 64, 97, 347
TseI GCWGC 3 cut(s) 34, 243, 653
Tsp45I GTSAC 3 cut(s) 64, 97, 347
TspDTI ATGAA 3 cut(s) 320, 485, 755
TspGWI ACGGA 2 cut(s) 294, 596
TspRI CASTG 1 cut(s) 681
XmiI GTMKAC 1 cut(s) 336
XmnI GAANNNNTTC 1 cut(s) 738
Zsp2I ATGCAT 1 cut(s) 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.