RLG00000014821

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000645
Physical Location & Seq
Forward (+)
58012630 .. 58015093
2464 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014821

Sequence Viewer

Length: 681 bp
ATGAATAACACCCCCCTACTCCACCACCTCGTCCTTCTTCTTCTGCTACTATACACCATCACCACCGTCACTTCATCCTCCGCCAACCAGGCTTACCCCACCCTCACCGAGGACTGCTCAGTGTCCGGTGAGGATATCCCTGTGCCCATACGGAGGGAGGTGTATGATAACGGTCGAATATTTGACATCAGCCACAGGTACACCCCTGACATGCCCAGTTATGGCGTTAAGGACGGGCTCGGGGAATTCCTGTCGCTTGGCATGAGCATCAAAAATGGCTCGGACGCCAACGTTTCCGTGATGAAGTTCAGTGTCCACTCCGGCACCCATGTGGACGCGCCGGGTCATGTCTTCGACCATTACTACGATGCTGGCTTTGATGTTGATGCGCTTGACCTCGAATTCTTAAATGGATTCTCTGTTAACATTACAAACATGTTGTTAGTCACCAAGGTAATGGCTAAATTAATTATAACTAGCCTTAGCCTTATGCAAATAAGAGAAAGAAGGCTTGATTACTTAAACGTCGCAGCTTCTGTTGATGTATTACCAGTTCATTATGCGTTTCTACAAGGCCGGGAATATACTTTTACGTTCATTGCTTGCTTTAAGGTTGATGGTTTTGATGGATCACAGCAAGATGCATTCTCATCAAATGAAACTTTTTCTAATCTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

227

Amino Acids

25.08

Weight (kDa)

4.87

Isoelectric Point (pI)

36.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 61 - 136 1.3e-07 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 473
AccB1I GGYRCC 1 cut(s) 323
AccII CGCG 1 cut(s) 338
AciI CCGC 1 cut(s) 81
AclI AACGTT 1 cut(s) 291
AclWI GGATC 1 cut(s) 637
AcsI RAATTY 2 cut(s) 245, 401
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 3 cut(s) 109, 153, 221
AflIII ACRYGT 1 cut(s) 435
AjnI CCWGG 1 cut(s) 87
AleI CACNNNNGTG 1 cut(s) 329
AluBI AGCT 1 cut(s) 533
AluI AGCT 1 cut(s) 533
AlwI GGATC 1 cut(s) 637
AlwNI CAGNNNCTG 1 cut(s) 536
Ama87I CYCGRG 1 cut(s) 239
AoxI GGCC 1 cut(s) 574
ApeKI GCWGC 1 cut(s) 530
ApoI RAATTY 2 cut(s) 245, 401
AseI ATTAAT 1 cut(s) 467
AspLEI GCGC 2 cut(s) 340, 391
AsuC2I CCSGG 2 cut(s) 342, 578
AsuHPI GGTGA 4 cut(s) 52, 97, 140, 439
AvaI CYCGRG 1 cut(s) 239
BaeGI GKGCMC 1 cut(s) 147
BanI GGYRCC 1 cut(s) 323
BanII GRGCYC 1 cut(s) 240
BbsI GAAGAC 1 cut(s) 343
BbvI GCAGC 1 cut(s) 542
BccI CCATC 3 cut(s) 65, 611, 620
BciT130I CCWGG 1 cut(s) 89
BcnI CCSGG 2 cut(s) 342, 578
BfaI CTAG 1 cut(s) 477
BglI GCCNNNNNGGC 1 cut(s) 89
BisI GCNGC 1 cut(s) 531
BlsI GCNGC 1 cut(s) 532
Bme1390I CCNGG 3 cut(s) 89, 342, 578
BmeT110I CYCGRG 1 cut(s) 239
BmiI GGNNCC 1 cut(s) 325
BmrFI CCNGG 3 cut(s) 89, 342, 578
BmrI ACTGGG 1 cut(s) 210
BmsI GCATC 4 cut(s) 276, 358, 376, 631
BmuI ACTGGG 1 cut(s) 210
BpiI GAAGAC 1 cut(s) 343
BplI GAGNNNNNCTC 2 cut(s) 101, 133
Bpu10I CCTNAGC 1 cut(s) 482
BpuMI CCSGG 2 cut(s) 342, 578
BsaHI GRCGYC 1 cut(s) 285
BsaJI CCNNGG 2 cut(s) 108, 450
BsaWI WCCGGW 1 cut(s) 125
Bsc4I CCNNNNNNNGG 3 cut(s) 109, 153, 221
Bse1I ACTGG 2 cut(s) 216, 551
Bse3DI GCAATG 1 cut(s) 597
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 2 cut(s) 108, 450
BseGI GGATG 1 cut(s) 74
BseLI CCNNNNNNNGG 3 cut(s) 109, 153, 221
BseMI GCAATG 1 cut(s) 597
BseMII CTCAG 1 cut(s) 132
BseNI ACTGG 2 cut(s) 216, 551
BseSI GKGCMC 1 cut(s) 147
BseXI GCAGC 1 cut(s) 542
Bsh1236I CGCG 1 cut(s) 338
Bsh1285I CGRYCG 1 cut(s) 175
BshFI GGCC 1 cut(s) 576
BshNI GGYRCC 1 cut(s) 323
BsiEI CGRYCG 1 cut(s) 175
BsiHKCI CYCGRG 1 cut(s) 239
BsiSI CCGG 4 cut(s) 126, 321, 341, 577
BslI CCNNNNNNNGG 3 cut(s) 109, 153, 221
BsmI GAATGC 1 cut(s) 644
BsnI GGCC 1 cut(s) 576
BsoBI CYCGRG 1 cut(s) 239
Bsp1286I GDGCHC 2 cut(s) 147, 240
Bsp143I GATC 1 cut(s) 629
BspACI CCGC 1 cut(s) 81
BspANI GGCC 1 cut(s) 576
BspCNI CTCAG 1 cut(s) 131
BspFNI CGCG 1 cut(s) 338
BspLI GGNNCC 1 cut(s) 325
BspPI GGATC 1 cut(s) 637
BspT107I GGYRCC 1 cut(s) 323
BsrDI GCAATG 1 cut(s) 597
BsrI ACTGG 2 cut(s) 216, 551
BssECI CCNNGG 2 cut(s) 108, 450
BssMI GATC 1 cut(s) 629
BssNI GRCGYC 1 cut(s) 285
BssT1I CCWWGG 1 cut(s) 450
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 2 cut(s) 67, 173
BstACI GRCGYC 1 cut(s) 285
BstC8I GCNNGC 2 cut(s) 373, 604
BstDEI CTNAG 2 cut(s) 118, 482
BstENI CCTNNNNNAGG 1 cut(s) 107
BstF5I GGATG 1 cut(s) 74
BstFNI CGCG 1 cut(s) 338
BstHHI GCGC 2 cut(s) 340, 391
BstKTI GATC 1 cut(s) 632
BstMBI GATC 1 cut(s) 629
BstMCI CGRYCG 1 cut(s) 175
BstMWI GCNNNNNNNGC 1 cut(s) 89
BstNI CCWGG 1 cut(s) 89
BstNSI RCATGY 2 cut(s) 214, 439
BstSCI CCNGG 3 cut(s) 87, 340, 576
BstSLI GKGCMC 1 cut(s) 147
BstUI CGCG 1 cut(s) 338
BstV1I GCAGC 1 cut(s) 542
BstV2I GAAGAC 1 cut(s) 343
BstXI CCANNNNNNTGG 1 cut(s) 457
BsuRI GGCC 1 cut(s) 576
BtsCI GGATG 1 cut(s) 74
BtsIMutI CAGTG 2 cut(s) 126, 316
Cac8I GCNNGC 2 cut(s) 373, 604
CaiI CAGNNNCTG 1 cut(s) 536
CfoI GCGC 2 cut(s) 340, 391
CseI GACGC 2 cut(s) 293, 344
Csp6I GTAC 1 cut(s) 199
CviAII CATG 5 cut(s) 211, 262, 329, 347, 436
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 2 cut(s) 118, 482
DpnI GATC 1 cut(s) 631
DpnII GATC 1 cut(s) 629
EciI GGCGGA 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 450
Eco24I GRGCYC 1 cut(s) 240
Eco32I GATATC 1 cut(s) 136
Eco88I CYCGRG 1 cut(s) 239
EcoNI CCTNNNNNAGG 1 cut(s) 107
EcoRI GAATTC 2 cut(s) 245, 401
EcoRII CCWGG 1 cut(s) 87
EcoRV GATATC 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 450
EcoT22I ATGCAT 1 cut(s) 646
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 450
FaeI CATG 5 cut(s) 214, 265, 332, 350, 439
FatI CATG 5 cut(s) 210, 261, 328, 346, 435
Fnu4HI GCNGC 1 cut(s) 531
FokI GGATG 1 cut(s) 61
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 1 cut(s) 531
FspBI CTAG 1 cut(s) 477
GlaI GCGC 2 cut(s) 339, 390
GluI GCNGC 1 cut(s) 531
HaeIII GGCC 1 cut(s) 576
HapII CCGG 4 cut(s) 126, 321, 341, 577
HgaI GACGC 2 cut(s) 293, 344
HhaI GCGC 2 cut(s) 340, 391
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 5 cut(s) 214, 265, 332, 350, 439
Hin6I GCGC 2 cut(s) 338, 389
HinP1I GCGC 2 cut(s) 338, 389
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HinfI GANTC 1 cut(s) 414
HpaI GTTAAC 1 cut(s) 424
HpaII CCGG 4 cut(s) 126, 321, 341, 577
HphI GGTGA 4 cut(s) 52, 97, 140, 439
Hpy166II GTNNAC 4 cut(s) 201, 316, 334, 424
Hpy188I TCNGA 1 cut(s) 283
Hpy8I GTNNAC 4 cut(s) 201, 316, 334, 424
Hpy99I CGWCG 1 cut(s) 530
HpyAV CCTTC 2 cut(s) 44, 501
HpyCH4III ACNGT 2 cut(s) 67, 173
HpyCH4IV ACGT 3 cut(s) 291, 525, 593
HpyCH4V TGCA 3 cut(s) 493, 644, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 89
HpyF3I CTNAG 2 cut(s) 118, 482
HpySE526I ACGT 3 cut(s) 291, 525, 593
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 5 cut(s) 214, 265, 332, 350, 439
HspAI GCGC 2 cut(s) 338, 389
KspAI GTTAAC 1 cut(s) 424
Kzo9I GATC 1 cut(s) 629
Lsp1109I GCAGC 1 cut(s) 542
LweI GCATC 4 cut(s) 276, 358, 376, 631
MaeI CTAG 1 cut(s) 477
MaeII ACGT 3 cut(s) 291, 525, 593
MaeIII GTNAC 2 cut(s) 67, 445
MalI GATC 1 cut(s) 631
MboI GATC 1 cut(s) 629
MboII GAAGA 3 cut(s) 29, 32, 343
MhlI GDGCHC 2 cut(s) 147, 240
MluCI AATT 4 cut(s) 245, 401, 464, 468
MnlI CCTC 8 cut(s) 38, 88, 103, 113, 124, 147, 151, 407
Mph1103I ATGCAT 1 cut(s) 646
MseI TTAA 6 cut(s) 228, 407, 423, 467, 521, 609
MslI CAYNNNNRTG 1 cut(s) 329
MspI CCGG 4 cut(s) 126, 321, 341, 577
MspR9I CCNGG 3 cut(s) 89, 342, 578
Mva1269I GAATGC 1 cut(s) 644
MvaI CCWGG 1 cut(s) 89
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 1 cut(s) 89
NciI CCSGG 2 cut(s) 342, 578
NdeII GATC 1 cut(s) 629
NlaIII CATG 5 cut(s) 214, 265, 332, 350, 439
NlaIV GGNNCC 1 cut(s) 325
NmuCI GTSAC 2 cut(s) 67, 445
NsiI ATGCAT 1 cut(s) 646
NspI RCATGY 2 cut(s) 214, 439
OliI CACNNNNGTG 1 cut(s) 329
PciI ACATGT 1 cut(s) 435
PctI GAATGC 1 cut(s) 644
PfeI GAWTC 1 cut(s) 414
PkrI GCNGC 1 cut(s) 532
PscI ACATGT 1 cut(s) 435
PshBI ATTAAT 1 cut(s) 467
PsiI TTATAA 1 cut(s) 473
Psp1406I AACGTT 1 cut(s) 291
Psp6I CCWGG 1 cut(s) 87
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 1 cut(s) 325
PsrI GAACNNNNNNTAC 2 cut(s) 537, 569
PstNI CAGNNNCTG 1 cut(s) 536
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
RseI CAYNNNNRTG 1 cut(s) 329
SaqAI TTAA 6 cut(s) 228, 407, 423, 467, 521, 609
SatI GCNGC 1 cut(s) 531
Sau3AI GATC 1 cut(s) 629
ScrFI CCNGG 3 cut(s) 89, 342, 578
SduI GDGCHC 2 cut(s) 147, 240
SfaNI GCATC 4 cut(s) 276, 358, 376, 631
SmiMI CAYNNNNRTG 1 cut(s) 329
Sse9I AATT 4 cut(s) 245, 401, 464, 468
SsiI CCGC 1 cut(s) 81
SspI AATATT 1 cut(s) 180
SspMI CTAG 1 cut(s) 477
StyD4I CCNGG 3 cut(s) 87, 340, 576
StyI CCWWGG 1 cut(s) 450
TaaI ACNGT 2 cut(s) 67, 173
TaiI ACGT 3 cut(s) 294, 528, 596
TaqI TCGA 3 cut(s) 175, 354, 399
TasI AATT 4 cut(s) 245, 401, 464, 468
TfiI GAWTC 1 cut(s) 414
Tru1I TTAA 6 cut(s) 228, 407, 423, 467, 521, 609
Tru9I TTAA 6 cut(s) 228, 407, 423, 467, 521, 609
TscAI CASTG 2 cut(s) 126, 316
TseFI GTSAC 2 cut(s) 67, 445
TseI GCWGC 1 cut(s) 530
Tsp45I GTSAC 2 cut(s) 67, 445
TspDTI ATGAA 6 cut(s) 17, 63, 317, 545, 586, 672
TspGWI ACGGA 2 cut(s) 166, 286
TspRI CASTG 2 cut(s) 126, 316
VspI ATTAAT 1 cut(s) 467
XagI CCTNNNNNAGG 1 cut(s) 107
XapI RAATTY 2 cut(s) 245, 401
XceI RCATGY 2 cut(s) 214, 439
XspI CTAG 1 cut(s) 477
Zsp2I ATGCAT 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.