RchiOBHm_Chr6g0255621

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000645
Physical Location & Seq
Forward (+)
11019806 .. 11025260
5455 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22926

Sequence Viewer

Length: 837 bp
ATGAAGACCTTACTCTTTCTCCTCCTACTCTGCTTCCTTTCGCTCACACACCCCACTCTCTCCGACGCCACCTCAGCCTATCCTTCCGTTCCCGGTACCGCCCCCGCCGACTGTTCTTTCTCCGACGGCCATGAGGCCCATGACACTGCCCCTCTGATTCCCCCTCGCCGTGAAGTGTATGGAAATGGCCGAATCTTCGACATAACCCACCTGATCACCACGGCCACGCCATCGTATGGCTCCCCGGACGGCGTGCTGGGTCAGTTCCTGTGGTTCCCCCACAGAAAGAAGAATGGCTCCATCGCGTCAGTTTTCAAGCTGCCTTCTCACACCGGCACGCATGTTGATTCCCCTGGACACTACTTCCATCACTACCTCGATGCTGGCTTCGATGTCGATACGCTTGACTTGGAGGTCCTTAATGGTCCAGCATTGTTAGTTGATGTTCCAAGGGACAAGAACATAACTGCTGAAGTGATGAAGTCCTTAAATATACCAAAGGGAGTACGTCATGTGCTCTTTAGAACATTAAATACTGACAGACGGCTTATGTTCAAAAATCAGTTTGACCCAAGCTATGTGGGATTTATGAAGGATGGAGCCAAGTGGTTGGTGGAAAACACTGACATCAAGCTTGTTGGAATTGATTACTTATCTGCTGCTGCATTTGATGATTTGATTCCATCGCACCTTGTTTTTCTAGAAAGTAGGGAAATCATTCTTGTGGAAGCCCTAAAACTTGATGACATCCAACCAGGAATATATTCAGTCCATTGCTTACCTTTGAGGTTGCAGGGTGCAGAAGGATCACCAATCAGATGCATTCTTATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

278

Amino Acids

30.79

Weight (kDa)

6.09

Isoelectric Point (pI)

31.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 66 - 220 2.1e-19 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 413
Acc65I GGTACC 1 cut(s) 95
AccB1I GGYRCC 1 cut(s) 95
AccB7I CCANNNNNTGG 1 cut(s) 236
AccII CGCG 1 cut(s) 305
AciI CCGC 2 cut(s) 99, 105
AclWI GGATC 1 cut(s) 814
AcoI YGGCCR 3 cut(s) 127, 187, 222
AcuI CTGAAG 1 cut(s) 492
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 2 cut(s) 97, 507
AfiI CCNNNNNNNGG 1 cut(s) 236
AgsI TTSAA 2 cut(s) 316, 556
AjnI CCWGG 2 cut(s) 352, 754
AluBI AGCT 3 cut(s) 319, 576, 634
AluI AGCT 3 cut(s) 319, 576, 634
Alw21I GWGCWC 1 cut(s) 519
AlwI GGATC 1 cut(s) 814
AlwNI CAGNNNCTG 1 cut(s) 268
AoxI GGCC 4 cut(s) 127, 135, 187, 222
ApeKI GCWGC 3 cut(s) 319, 659, 662
Asp700I GAANNNNTTC 2 cut(s) 717, 763
Asp718I GGTACC 1 cut(s) 95
AspS9I GGNCC 3 cut(s) 136, 415, 425
AsuC2I CCSGG 2 cut(s) 93, 245
AsuHPI GGTGA 2 cut(s) 208, 801
AvaII GGWCC 2 cut(s) 415, 425
BanI GGYRCC 1 cut(s) 95
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 519
BbvCI CCTCAGC 1 cut(s) 73
BbvI GCAGC 3 cut(s) 306, 646, 649
BccI CCATC 5 cut(s) 238, 308, 375, 590, 691
BceAI ACGGC 5 cut(s) 142, 153, 237, 265, 560
BciT130I CCWGG 2 cut(s) 354, 756
BclI TGATCA 1 cut(s) 213
BcnI CCSGG 2 cut(s) 93, 245
BfaI CTAG 1 cut(s) 701
BisI GCNGC 3 cut(s) 320, 660, 663
BlsI GCNGC 3 cut(s) 321, 661, 664
Bme1390I CCNGG 4 cut(s) 93, 245, 354, 756
Bme18I GGWCC 2 cut(s) 415, 425
BmgT120I GGNCC 3 cut(s) 136, 415, 425
BmiI GGNNCC 5 cut(s) 97, 241, 275, 298, 601
BmrFI CCNGG 4 cut(s) 93, 245, 354, 756
BmsI GCATC 2 cut(s) 370, 809
BpiI GAAGAC 1 cut(s) 11
Bpu10I CCTNAGC 1 cut(s) 73
BpuMI CCSGG 2 cut(s) 93, 245
BsaHI GRCGYC 1 cut(s) 66
BsaJI CCNNGG 4 cut(s) 219, 243, 352, 449
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse118I RCCGGY 1 cut(s) 332
Bse3DI GCAATG 1 cut(s) 772
BseBI CCWGG 2 cut(s) 354, 756
BseDI CCNNGG 4 cut(s) 219, 243, 352, 449
BseGI GGATG 2 cut(s) 601, 747
BseLI CCNNNNNNNGG 1 cut(s) 236
BseMI GCAATG 1 cut(s) 772
BseMII CTCAG 1 cut(s) 87
BseRI GAGGAG 1 cut(s) 11
BseXI GCAGC 3 cut(s) 306, 646, 649
BseYI CCCAGC 1 cut(s) 256
BsgI GTGCAG 1 cut(s) 819
Bsh1236I CGCG 1 cut(s) 305
BshFI GGCC 4 cut(s) 129, 137, 189, 224
BshNI GGYRCC 1 cut(s) 95
BsiHKAI GWGCWC 1 cut(s) 519
BsiSI CCGG 3 cut(s) 93, 245, 333
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 1 cut(s) 236
BsmFI GGGAC 1 cut(s) 467
BsmI GAATGC 1 cut(s) 822
BsnI GGCC 4 cut(s) 129, 137, 189, 224
Bsp1286I GDGCHC 1 cut(s) 519
Bsp143I GATC 2 cut(s) 213, 806
BspACI CCGC 2 cut(s) 99, 105
BspANI GGCC 4 cut(s) 129, 137, 189, 224
BspCNI CTCAG 1 cut(s) 86
BspFNI CGCG 1 cut(s) 305
BspLI GGNNCC 5 cut(s) 97, 241, 275, 298, 601
BspPI GGATC 1 cut(s) 814
BspT107I GGYRCC 1 cut(s) 95
BsrDI GCAATG 1 cut(s) 772
BsrFI RCCGGY 1 cut(s) 332
BssAI RCCGGY 1 cut(s) 332
BssECI CCNNGG 4 cut(s) 219, 243, 352, 449
BssMI GATC 2 cut(s) 213, 806
BssNI GRCGYC 1 cut(s) 66
BssT1I CCWWGG 1 cut(s) 449
Bst2UI CCWGG 2 cut(s) 354, 756
Bst4CI ACNGT 1 cut(s) 113
BstACI GRCGYC 1 cut(s) 66
BstC8I GCNNGC 3 cut(s) 254, 338, 385
BstDEI CTNAG 1 cut(s) 73
BstDSI CCRYGG 1 cut(s) 219
BstF5I GGATG 2 cut(s) 601, 747
BstFNI CGCG 1 cut(s) 305
BstKTI GATC 2 cut(s) 216, 809
BstMBI GATC 2 cut(s) 213, 806
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstNI CCWGG 2 cut(s) 354, 756
BstNSI RCATGY 1 cut(s) 344
BstSCI CCNGG 4 cut(s) 91, 243, 352, 754
BstUI CGCG 1 cut(s) 305
BstV1I GCAGC 3 cut(s) 306, 646, 649
BstV2I GAAGAC 1 cut(s) 11
BstXI CCANNNNNNTGG 1 cut(s) 610
BsuRI GGCC 4 cut(s) 129, 137, 189, 224
BtgI CCRYGG 1 cut(s) 219
BtgZI GCGATG 2 cut(s) 286, 669
BtsCI GGATG 2 cut(s) 601, 747
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 2 cut(s) 144, 621
Cac8I GCNNGC 3 cut(s) 254, 338, 385
CaiI CAGNNNCTG 1 cut(s) 268
Cfr10I RCCGGY 1 cut(s) 332
Cfr13I GGNCC 3 cut(s) 136, 415, 425
CseI GACGC 2 cut(s) 74, 294
Csp6I GTAC 2 cut(s) 96, 506
CspCI CAANNNNNGTGG 2 cut(s) 561, 596
CviAII CATG 4 cut(s) 131, 140, 341, 512
CviQI GTAC 2 cut(s) 96, 506
DdeI CTNAG 1 cut(s) 73
DpnI GATC 2 cut(s) 215, 808
DpnII GATC 2 cut(s) 213, 806
DrdI GACNNNNNNGTC 1 cut(s) 413
DseDI GACNNNNNNGTC 1 cut(s) 413
EaeI YGGCCR 3 cut(s) 127, 187, 222
Eco130I CCWWGG 1 cut(s) 449
Eco47I GGWCC 2 cut(s) 415, 425
Eco57I CTGAAG 1 cut(s) 492
EcoO109I RGGNCCY 1 cut(s) 415
EcoRII CCWGG 2 cut(s) 352, 754
EcoT14I CCWWGG 1 cut(s) 449
EcoT22I ATGCAT 1 cut(s) 824
ErhI CCWWGG 1 cut(s) 449
FaeI CATG 4 cut(s) 134, 143, 344, 515
FaqI GGGAC 1 cut(s) 467
FatI CATG 4 cut(s) 130, 139, 340, 511
FauI CCCGC 1 cut(s) 112
FbaI TGATCA 1 cut(s) 213
Fnu4HI GCNGC 3 cut(s) 320, 660, 663
FokI GGATG 2 cut(s) 608, 734
Fsp4HI GCNGC 3 cut(s) 320, 660, 663
FspBI CTAG 1 cut(s) 701
GluI GCNGC 3 cut(s) 320, 660, 663
GsaI CCCAGC 1 cut(s) 260
HaeIII GGCC 4 cut(s) 129, 137, 189, 224
HapII CCGG 3 cut(s) 93, 245, 333
HgaI GACGC 2 cut(s) 74, 294
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 4 cut(s) 134, 143, 344, 515
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 4 cut(s) 157, 192, 347, 679
HpaII CCGG 3 cut(s) 93, 245, 333
HphI GGTGA 2 cut(s) 208, 801
Hpy188I TCNGA 4 cut(s) 64, 124, 156, 818
Hpy188III TCNNGA 1 cut(s) 701
Hpy99I CGWCG 2 cut(s) 68, 128
HpyAV CCTTC 4 cut(s) 93, 333, 586, 797
HpyCH4III ACNGT 1 cut(s) 113
HpyCH4IV ACGT 1 cut(s) 508
HpyCH4V TGCA 4 cut(s) 665, 793, 800, 822
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpyF3I CTNAG 1 cut(s) 73
HpySE526I ACGT 1 cut(s) 508
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 4 cut(s) 134, 143, 344, 515
KpnI GGTACC 1 cut(s) 99
Ksp22I TGATCA 1 cut(s) 213
Kzo9I GATC 2 cut(s) 213, 806
LmnI GCTCC 3 cut(s) 245, 302, 599
Lsp1109I GCAGC 3 cut(s) 306, 646, 649
LweI GCATC 2 cut(s) 370, 809
MaeI CTAG 1 cut(s) 701
MaeII ACGT 1 cut(s) 508
MalI GATC 2 cut(s) 215, 808
MboI GATC 2 cut(s) 213, 806
MboII GAAGA 3 cut(s) 16, 187, 301
MhlI GDGCHC 1 cut(s) 519
MluCI AATT 1 cut(s) 642
MmeI TCCRAC 4 cut(s) 87, 147, 619, 775
MnlI CCTC 8 cut(s) 32, 82, 127, 162, 174, 386, 406, 780
Mph1103I ATGCAT 1 cut(s) 824
MroXI GAANNNNTTC 2 cut(s) 717, 763
MseI TTAA 3 cut(s) 420, 488, 530
MslI CAYNNNNRTG 1 cut(s) 722
MspI CCGG 3 cut(s) 93, 245, 333
MspR9I CCNGG 4 cut(s) 93, 245, 354, 756
Mva1269I GAATGC 1 cut(s) 822
MvaI CCWGG 2 cut(s) 354, 756
MvnI CGCG 1 cut(s) 305
MwoI GCNNNNNNNGC 1 cut(s) 74
NciI CCSGG 2 cut(s) 93, 245
NdeII GATC 2 cut(s) 213, 806
NlaIII CATG 4 cut(s) 134, 143, 344, 515
NlaIV GGNNCC 5 cut(s) 97, 241, 275, 298, 601
NsiI ATGCAT 1 cut(s) 824
NspI RCATGY 1 cut(s) 344
PctI GAATGC 1 cut(s) 822
PdmI GAANNNNTTC 2 cut(s) 717, 763
PfeI GAWTC 4 cut(s) 157, 192, 347, 679
PflMI CCANNNNNTGG 1 cut(s) 236
PkrI GCNGC 3 cut(s) 321, 661, 664
PpuMI RGGWCCY 1 cut(s) 415
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 2 cut(s) 352, 754
PspFI CCCAGC 1 cut(s) 256
PspGI CCWGG 2 cut(s) 352, 754
PspN4I GGNNCC 5 cut(s) 97, 241, 275, 298, 601
PspPI GGNCC 3 cut(s) 136, 415, 425
PspPPI RGGWCCY 1 cut(s) 415
PsrI GAACNNNNNNTAC 2 cut(s) 517, 549
PstNI CAGNNNCTG 1 cut(s) 268
RsaI GTAC 2 cut(s) 97, 507
RsaNI GTAC 2 cut(s) 96, 506
RseI CAYNNNNRTG 1 cut(s) 722
SaqAI TTAA 3 cut(s) 420, 488, 530
SatI GCNGC 3 cut(s) 320, 660, 663
Sau3AI GATC 2 cut(s) 213, 806
Sau96I GGNCC 3 cut(s) 136, 415, 425
ScrFI CCNGG 4 cut(s) 93, 245, 354, 756
SduI GDGCHC 1 cut(s) 519
SfaNI GCATC 2 cut(s) 370, 809
SinI GGWCC 2 cut(s) 415, 425
SmiMI CAYNNNNRTG 1 cut(s) 722
Sse9I AATT 1 cut(s) 642
SsiI CCGC 2 cut(s) 99, 105
SspMI CTAG 1 cut(s) 701
StyD4I CCNGG 4 cut(s) 91, 243, 352, 754
StyI CCWWGG 1 cut(s) 449
TaaI ACNGT 1 cut(s) 113
TaiI ACGT 1 cut(s) 511
TaqI TCGA 4 cut(s) 198, 378, 390, 396
TasI AATT 1 cut(s) 642
TfiI GAWTC 4 cut(s) 157, 192, 347, 679
Tru1I TTAA 3 cut(s) 420, 488, 530
Tru9I TTAA 3 cut(s) 420, 488, 530
TscAI CASTG 2 cut(s) 151, 628
TseI GCWGC 3 cut(s) 319, 659, 662
TspDTI ATGAA 3 cut(s) 17, 494, 605
TspGWI ACGGA 1 cut(s) 76
TspRI CASTG 2 cut(s) 151, 628
Van91I CCANNNNNTGG 1 cut(s) 236
VpaK11BI GGWCC 2 cut(s) 415, 425
XbaI TCTAGA 1 cut(s) 700
XceI RCATGY 1 cut(s) 344
XcmI CCANNNNNNNNNTGG 1 cut(s) 610
XmnI GAANNNNTTC 2 cut(s) 717, 763
XspI CTAG 1 cut(s) 701
Zsp2I ATGCAT 1 cut(s) 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.