Rh6DG066400

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000645
Physical Location & Seq
Forward (+)
6767223 .. 6772524
5302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG066400.1

Sequence Viewer

Length: 546 bp
ATGAAGGCCTTACTCTTTCTCCTCCTACTCTGCTCCCTTACGCTCGCAATCCCCACTCTCTCCCACACCACCACCGCCTACCCTTCCGTTCCCGGCACCGCCGCCGCCGACTGTTCTTTCTCCGACGGCGACACCGCCCCTCTGATTCCCCCTCGCCACGAAGTGTATGGAAATGGCCGAATCTTCGACATCACCCACCGTATCACCACGGCGATGCCGTCGTATTTCTCCCCGGACGGCGTGCTGGGCCAGTTCCTGTGGCTCCCCCACAGCATGAAGAACGGCTCCATCGCTAACATGTCAGTTTTCAAGCTGCCTACTCACACCGGCACGCATATTGATTCACCCGGACACTTCTTCGATCACTACCTCGATGCTGGCTTCGACGTCGATACGCTTGACTTGGAGGTGCTTAATGGTCCAGCATTGTTAGTTGATGTTCCGAGGGACAAGAACATAACTGGAAACAGCAACAGCCCCAGGAAAAGTTGCATAGCAATGAAACGGACAGTATCCGAGTTCTTGCAAGGCATGGAAAAGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

181

Amino Acids

19.69

Weight (kDa)

6.01

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 62 - 151 2.2e-11 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 390
AccB1I GGYRCC 1 cut(s) 95
AciI CCGC 5 cut(s) 75, 99, 102, 105, 135
AcoI YGGCCR 1 cut(s) 175
AcyI GRCGYC 1 cut(s) 387
AdeI CACNNNGTG 1 cut(s) 163
AflIII ACRYGT 1 cut(s) 297
AgsI TTSAA 1 cut(s) 310
AjiI CACGTC 1 cut(s) 543
AjnI CCWGG 1 cut(s) 479
AluBI AGCT 1 cut(s) 313
AluI AGCT 1 cut(s) 313
AlwNI CAGNNNCTG 1 cut(s) 256
AoxI GGCC 3 cut(s) 6, 175, 247
ApeKI GCWGC 1 cut(s) 313
AspS9I GGNCC 2 cut(s) 247, 419
AsuC2I CCSGG 3 cut(s) 93, 233, 348
AsuHPI GGTGA 3 cut(s) 184, 196, 336
AvaII GGWCC 1 cut(s) 419
BanI GGYRCC 1 cut(s) 95
BbvI GCAGC 1 cut(s) 300
BccI CCATC 1 cut(s) 296
BceAI ACGGC 5 cut(s) 142, 202, 225, 253, 298
BciT130I CCWGG 1 cut(s) 481
BciVI GTATCC 1 cut(s) 523
BcnI CCSGG 3 cut(s) 93, 233, 348
BfuI GTATCC 1 cut(s) 523
BisI GCNGC 3 cut(s) 102, 105, 314
BlsI GCNGC 3 cut(s) 103, 106, 315
Bme1390I CCNGG 4 cut(s) 93, 233, 348, 481
Bme18I GGWCC 1 cut(s) 419
BmgBI CACGTC 1 cut(s) 543
BmgT120I GGNCC 2 cut(s) 247, 419
BmiI GGNNCC 3 cut(s) 97, 263, 286
BmrFI CCNGG 4 cut(s) 93, 233, 348, 481
BmsI GCATC 2 cut(s) 204, 364
BpuMI CCSGG 3 cut(s) 93, 233, 348
BsaHI GRCGYC 1 cut(s) 387
BsaJI CCNNGG 4 cut(s) 207, 231, 443, 479
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bse118I RCCGGY 1 cut(s) 326
Bse1I ACTGG 2 cut(s) 250, 466
Bse3DI GCAATG 1 cut(s) 504
BseBI CCWGG 1 cut(s) 481
BseDI CCNNGG 4 cut(s) 207, 231, 443, 479
BseMI GCAATG 1 cut(s) 504
BseNI ACTGG 2 cut(s) 250, 466
BseRI GAGGAG 1 cut(s) 11
BseXI GCAGC 1 cut(s) 300
BseYI CCCAGC 1 cut(s) 244
BshFI GGCC 3 cut(s) 8, 177, 249
BshNI GGYRCC 1 cut(s) 95
BsiSI CCGG 4 cut(s) 93, 233, 327, 348
BslFI GGGAC 1 cut(s) 461
BsmFI GGGAC 1 cut(s) 461
BsnI GGCC 3 cut(s) 8, 177, 249
Bsp143I GATC 1 cut(s) 361
BspACI CCGC 5 cut(s) 75, 99, 102, 105, 135
BspANI GGCC 3 cut(s) 8, 177, 249
BspLI GGNNCC 3 cut(s) 97, 263, 286
BspT107I GGYRCC 1 cut(s) 95
BsrDI GCAATG 1 cut(s) 504
BsrFI RCCGGY 1 cut(s) 326
BsrI ACTGG 2 cut(s) 250, 466
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 4 cut(s) 207, 231, 443, 479
BssMI GATC 1 cut(s) 361
BssNI GRCGYC 1 cut(s) 387
Bst2UI CCWGG 1 cut(s) 481
Bst4CI ACNGT 3 cut(s) 113, 200, 511
BstACI GRCGYC 1 cut(s) 387
BstC8I GCNNGC 4 cut(s) 45, 242, 332, 379
BstDSI CCRYGG 1 cut(s) 207
BstKTI GATC 1 cut(s) 364
BstMBI GATC 1 cut(s) 361
BstMWI GCNNNNNNNGC 1 cut(s) 246
BstNI CCWGG 1 cut(s) 481
BstNSI RCATGY 1 cut(s) 301
BstSCI CCNGG 4 cut(s) 91, 231, 346, 479
BstV1I GCAGC 1 cut(s) 300
BsuI GTATCC 1 cut(s) 523
BsuRI GGCC 3 cut(s) 8, 177, 249
BtgI CCRYGG 1 cut(s) 207
BtgZI GCGATG 2 cut(s) 227, 274
BtrI CACGTC 1 cut(s) 543
Cac8I GCNNGC 4 cut(s) 45, 242, 332, 379
CaiI CAGNNNCTG 1 cut(s) 256
Cfr10I RCCGGY 1 cut(s) 326
Cfr13I GGNCC 2 cut(s) 247, 419
CviAII CATG 3 cut(s) 274, 298, 532
CviJI RGCY 8 cut(s) 8, 177, 249, 262, 285, 313, 381, 477
CviKI_1 RGCY 8 cut(s) 8, 177, 249, 262, 285, 313, 381, 477
DpnI GATC 1 cut(s) 363
DpnII GATC 1 cut(s) 361
DraIII CACNNNGTG 1 cut(s) 163
EaeI YGGCCR 1 cut(s) 175
Eco147I AGGCCT 1 cut(s) 8
Eco47I GGWCC 1 cut(s) 419
EcoRII CCWGG 1 cut(s) 479
FaeI CATG 3 cut(s) 277, 301, 535
FaiI YATR 7 cut(s) 168, 275, 299, 336, 458, 494, 533
FaqI GGGAC 1 cut(s) 461
FatI CATG 3 cut(s) 273, 297, 531
Fnu4HI GCNGC 3 cut(s) 102, 105, 314
Fsp4HI GCNGC 3 cut(s) 102, 105, 314
GluI GCNGC 3 cut(s) 102, 105, 314
GsaI CCCAGC 1 cut(s) 248
HaeIII GGCC 3 cut(s) 8, 177, 249
HapII CCGG 4 cut(s) 93, 233, 327, 348
Hin1I GRCGYC 1 cut(s) 387
Hin1II CATG 3 cut(s) 277, 301, 535
HinfI GANTC 3 cut(s) 145, 180, 341
HpaII CCGG 4 cut(s) 93, 233, 327, 348
HphI GGTGA 3 cut(s) 184, 196, 336
Hpy188I TCNGA 4 cut(s) 124, 144, 444, 517
Hpy99I CGWCG 4 cut(s) 128, 223, 389, 392
HpyAV CCTTC 1 cut(s) 93
HpyCH4III ACNGT 3 cut(s) 113, 200, 511
HpyCH4IV ACGT 2 cut(s) 387, 542
HpyCH4V TGCA 2 cut(s) 492, 526
HpyF10VI GCNNNNNNNGC 1 cut(s) 246
HpySE526I ACGT 2 cut(s) 387, 542
Hsp92I GRCGYC 1 cut(s) 387
Hsp92II CATG 3 cut(s) 277, 301, 535
Kzo9I GATC 1 cut(s) 361
LmnI GCTCC 3 cut(s) 38, 267, 290
Lsp1109I GCAGC 1 cut(s) 300
LweI GCATC 2 cut(s) 204, 364
MaeII ACGT 2 cut(s) 387, 542
MalI GATC 1 cut(s) 363
MboI GATC 1 cut(s) 361
MboII GAAGA 3 cut(s) 175, 289, 349
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 6 cut(s) 32, 150, 162, 380, 400, 438
MseI TTAA 1 cut(s) 414
MslI CAYNNNNRTG 2 cut(s) 212, 497
MspI CCGG 4 cut(s) 93, 233, 327, 348
MspR9I CCNGG 4 cut(s) 93, 233, 348, 481
MvaI CCWGG 1 cut(s) 481
MwoI GCNNNNNNNGC 1 cut(s) 246
NciI CCSGG 3 cut(s) 93, 233, 348
NdeII GATC 1 cut(s) 361
NlaIII CATG 3 cut(s) 277, 301, 535
NlaIV GGNNCC 3 cut(s) 97, 263, 286
NspI RCATGY 1 cut(s) 301
PceI AGGCCT 1 cut(s) 8
PciI ACATGT 1 cut(s) 297
PcsI WCGNNNNNNNCGW 1 cut(s) 215
PfeI GAWTC 3 cut(s) 145, 180, 341
PkrI GCNGC 3 cut(s) 103, 106, 315
PscI ACATGT 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 479
PspFI CCCAGC 1 cut(s) 244
PspGI CCWGG 1 cut(s) 479
PspN4I GGNNCC 3 cut(s) 97, 263, 286
PspPI GGNCC 2 cut(s) 247, 419
PstNI CAGNNNCTG 1 cut(s) 256
RseI CAYNNNNRTG 2 cut(s) 212, 497
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 3 cut(s) 102, 105, 314
Sau3AI GATC 1 cut(s) 361
Sau96I GGNCC 2 cut(s) 247, 419
ScrFI CCNGG 4 cut(s) 93, 233, 348, 481
SetI ASST 5 cut(s) 315, 372, 390, 411, 545
SfaNI GCATC 2 cut(s) 204, 364
SinI GGWCC 1 cut(s) 419
SmiMI CAYNNNNRTG 2 cut(s) 212, 497
SseBI AGGCCT 1 cut(s) 8
SsiI CCGC 5 cut(s) 75, 99, 102, 105, 135
StuI AGGCCT 1 cut(s) 8
StyD4I CCNGG 4 cut(s) 91, 231, 346, 479
TaaI ACNGT 3 cut(s) 113, 200, 511
TaiI ACGT 2 cut(s) 390, 545
TaqI TCGA 5 cut(s) 186, 360, 372, 384, 390
TauI GCSGC 2 cut(s) 104, 107
TfiI GAWTC 3 cut(s) 145, 180, 341
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TseI GCWGC 1 cut(s) 313
TspDTI ATGAA 3 cut(s) 17, 290, 515
TspGWI ACGGA 2 cut(s) 76, 520
VpaK11BI GGWCC 1 cut(s) 419
XceI RCATGY 1 cut(s) 301
XcmI CCANNNNNNNNNTGG 1 cut(s) 164
ZraI GACGTC 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.