Prupe.8G133400_v2.0.a1

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000645
Physical Location & Seq
Reverse (-)
15489532 .. 15492650
3119 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G133400.1

Sequence Viewer

Length: 813 bp
ATGAAACCCACATTCCTCCTCCTCTTCTTCTTCCTTCTCTCCTCTATTTCCTTCTCCCACGCCACCACAGCCTACCCTTCTATTCCCGGCACCACCTCTGTAGAATACGGCGCCGACACCGACCCTCTGATTCCTCCTCGCCGCGAAGTGTATGGCAATGGCCGAATCTTCGACATCAGCCACCGCTACAGCACCAACATGCCCGCCTTTGAGAGCAACGACGGGGTTGGCGAGTTCCTGTGGCTACCCAACAGCATGAAGAACGGCTCCATCGCCAACAACTCAGAGTTTAAGCTGCCCACTCACACTGGTACCCATGTCGATGCCCCTGGCCACGTCTTCGATCACTACTTCGATGCCGGCTTCGACGTCGATACGCTTGACTTGGAAGTGCTTAATGGTCCTGCATTGTTAGTTGACGTTCCGAGGGATAAGAACATAACTGCTGAAGTTATGAAGTCCTTACATATTCCCAAGGGGGTACGTCGTGTGCTGTTTAGAACATTAAACACTGACAGACGGCTTATGTTCCAAAAGGAGTTTGACACCAGCTACGTGGGATTTATGAAAGATGGAGCACAGTGGTTGGTGGACAACACTGACATTAAACTTGTTGGAATTGATTACTTATCTGTTGCTGCATATGATGATTTGATTCCGTCCCACCTTGTTTTTCTAGAAGGCAGGGAAATCATTCTTGTTGAAGCCTTAAAACTTGATGACATCCAACCAGGAATATATTCTGTCCACTGCTTACCTTTGAGGTTGCCGGGTGCAGAAGGATCACCGATAAGATGCATTCTTATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

271

Amino Acids

30.19

Weight (kDa)

5.31

Isoelectric Point (pI)

27.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 372
Acc65I GGTACC 1 cut(s) 311
AccB1I GGYRCC 3 cut(s) 89, 110, 311
AccII CGCG 1 cut(s) 144
AciI CCGC 3 cut(s) 142, 184, 204
AclWI GGATC 1 cut(s) 790
AcoI YGGCCR 2 cut(s) 160, 331
AcuI CTGAAG 1 cut(s) 468
AcyI GRCGYC 2 cut(s) 111, 369
AfaI GTAC 2 cut(s) 313, 483
AgsI TTSAA 1 cut(s) 704
AjiI CACGTC 1 cut(s) 337
AjnI CCWGG 2 cut(s) 328, 730
AluBI AGCT 2 cut(s) 295, 552
AluI AGCT 2 cut(s) 295, 552
Alw21I GWGCWC 1 cut(s) 580
AlwI GGATC 1 cut(s) 790
AoxI GGCC 2 cut(s) 160, 331
ApeKI GCWGC 2 cut(s) 295, 638
Asp700I GAANNNNTTC 2 cut(s) 693, 739
Asp718I GGTACC 1 cut(s) 311
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 1 cut(s) 401
AsuC2I CCSGG 2 cut(s) 87, 771
AsuHPI GGTGA 1 cut(s) 777
AvaII GGWCC 1 cut(s) 401
BaeI ACNNNNGTAYC 4 cut(s) 295, 303, 328, 336
BalI TGGCCA 1 cut(s) 333
BanI GGYRCC 3 cut(s) 89, 110, 311
BbsI GAAGAC 1 cut(s) 331
Bbv12I GWGCWC 1 cut(s) 580
BbvI GCAGC 2 cut(s) 282, 625
BccI CCATC 2 cut(s) 278, 566
BceAI ACGGC 3 cut(s) 124, 280, 536
BciT130I CCWGG 2 cut(s) 330, 732
BcnI CCSGG 2 cut(s) 87, 771
BfaI CTAG 1 cut(s) 677
BfmI CTRYAG 2 cut(s) 99, 187
BfoI RGCGCY 1 cut(s) 114
BisI GCNGC 3 cut(s) 142, 296, 639
BlsI GCNGC 3 cut(s) 143, 297, 640
Bme1390I CCNGG 4 cut(s) 87, 330, 732, 771
Bme18I GGWCC 1 cut(s) 401
BmgBI CACGTC 1 cut(s) 337
BmgT120I GGNCC 1 cut(s) 401
BmiI GGNNCC 4 cut(s) 91, 112, 268, 313
BmrFI CCNGG 4 cut(s) 87, 330, 732, 771
BmsI GCATC 3 cut(s) 313, 346, 785
BpiI GAAGAC 1 cut(s) 331
BpuMI CCSGG 2 cut(s) 87, 771
BsaAI YACGTR 1 cut(s) 556
BsaHI GRCGYC 2 cut(s) 111, 369
BsaJI CCNNGG 3 cut(s) 328, 425, 474
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 1 cut(s) 313
Bse3DI GCAATG 1 cut(s) 163
BseBI CCWGG 2 cut(s) 330, 732
BseDI CCNNGG 3 cut(s) 328, 425, 474
BseGI GGATG 1 cut(s) 723
BseMI GCAATG 1 cut(s) 163
BseMII CTCAG 1 cut(s) 297
BseNI ACTGG 1 cut(s) 313
BseRI GAGGAG 4 cut(s) 8, 11, 31, 126
BseXI GCAGC 2 cut(s) 282, 625
BsgI GTGCAG 1 cut(s) 795
Bsh1236I CGCG 1 cut(s) 144
BshFI GGCC 2 cut(s) 162, 333
BshNI GGYRCC 3 cut(s) 89, 110, 311
BsiHKAI GWGCWC 1 cut(s) 580
BsiSI CCGG 3 cut(s) 87, 360, 770
BslFI GGGAC 1 cut(s) 646
BsmFI GGGAC 1 cut(s) 646
BsmI GAATGC 1 cut(s) 798
BsnI GGCC 2 cut(s) 162, 333
Bsp1286I GDGCHC 1 cut(s) 580
Bsp143I GATC 2 cut(s) 343, 782
BspACI CCGC 3 cut(s) 142, 184, 204
BspANI GGCC 2 cut(s) 162, 333
BspCNI CTCAG 1 cut(s) 296
BspFNI CGCG 1 cut(s) 144
BspLI GGNNCC 4 cut(s) 91, 112, 268, 313
BspPI GGATC 1 cut(s) 790
BspT107I GGYRCC 3 cut(s) 89, 110, 311
BsrDI GCAATG 1 cut(s) 163
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 1 cut(s) 313
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 3 cut(s) 328, 425, 474
BssMI GATC 2 cut(s) 343, 782
BssNI GRCGYC 2 cut(s) 111, 369
BssT1I CCWWGG 1 cut(s) 474
Bst2UI CCWGG 2 cut(s) 330, 732
Bst4CI ACNGT 1 cut(s) 582
Bst6I CTCTTC 1 cut(s) 29
BstACI GRCGYC 2 cut(s) 111, 369
BstBAI YACGTR 1 cut(s) 556
BstC8I GCNNGC 2 cut(s) 204, 361
BstDEI CTNAG 1 cut(s) 283
BstF5I GGATG 1 cut(s) 723
BstFNI CGCG 1 cut(s) 144
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 1 cut(s) 113
BstKTI GATC 2 cut(s) 346, 785
BstMBI GATC 2 cut(s) 343, 782
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNI CCWGG 2 cut(s) 330, 732
BstNSI RCATGY 1 cut(s) 202
BstSCI CCNGG 4 cut(s) 85, 328, 730, 769
BstSFI CTRYAG 2 cut(s) 99, 187
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 2 cut(s) 282, 625
BstV2I GAAGAC 1 cut(s) 331
BstXI CCANNNNNNTGG 1 cut(s) 556
BsuRI GGCC 2 cut(s) 162, 333
BtgZI GCGATG 1 cut(s) 256
BtrI CACGTC 1 cut(s) 337
BtsCI GGATG 1 cut(s) 723
BtsI GCAGTG 1 cut(s) 748
BtsIMutI CAGTG 5 cut(s) 306, 510, 587, 597, 748
Cac8I GCNNGC 2 cut(s) 204, 361
CfoI GCGC 1 cut(s) 113
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 1 cut(s) 401
Csp6I GTAC 2 cut(s) 312, 482
CviAII CATG 3 cut(s) 199, 256, 317
CviQI GTAC 2 cut(s) 312, 482
DdeI CTNAG 1 cut(s) 283
DinI GGCGCC 1 cut(s) 112
DpnI GATC 2 cut(s) 345, 784
DpnII GATC 2 cut(s) 343, 782
EaeI YGGCCR 2 cut(s) 160, 331
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco130I CCWWGG 1 cut(s) 474
Eco47I GGWCC 1 cut(s) 401
Eco57I CTGAAG 1 cut(s) 468
EcoRII CCWGG 2 cut(s) 328, 730
EcoT14I CCWWGG 1 cut(s) 474
EcoT22I ATGCAT 1 cut(s) 800
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 474
FaeI CATG 3 cut(s) 202, 259, 320
FaqI GGGAC 1 cut(s) 646
FatI CATG 3 cut(s) 198, 255, 316
FauI CCCGC 1 cut(s) 211
FauNDI CATATG 1 cut(s) 643
Fnu4HI GCNGC 3 cut(s) 142, 296, 639
FokI GGATG 1 cut(s) 710
Fsp4HI GCNGC 3 cut(s) 142, 296, 639
FspBI CTAG 1 cut(s) 677
GlaI GCGC 1 cut(s) 112
GluI GCNGC 3 cut(s) 142, 296, 639
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 2 cut(s) 162, 333
HapII CCGG 3 cut(s) 87, 360, 770
HhaI GCGC 1 cut(s) 113
Hin1I GRCGYC 2 cut(s) 111, 369
Hin1II CATG 3 cut(s) 202, 259, 320
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 3 cut(s) 130, 165, 655
HpaII CCGG 3 cut(s) 87, 360, 770
HphI GGTGA 1 cut(s) 777
Hpy166II GTNNAC 3 cut(s) 418, 592, 748
Hpy188I TCNGA 3 cut(s) 129, 286, 426
Hpy188III TCNNGA 1 cut(s) 677
Hpy8I GTNNAC 3 cut(s) 418, 592, 748
Hpy99I CGWCG 4 cut(s) 224, 371, 374, 489
HpyAV CCTTC 5 cut(s) 44, 61, 87, 674, 773
HpyCH4III ACNGT 1 cut(s) 582
HpyCH4IV ACGT 5 cut(s) 336, 369, 420, 484, 555
HpyCH4V TGCA 4 cut(s) 407, 641, 776, 798
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 1 cut(s) 283
HpySE526I ACGT 5 cut(s) 336, 369, 420, 484, 555
Hsp92I GRCGYC 2 cut(s) 111, 369
Hsp92II CATG 3 cut(s) 202, 259, 320
HspAI GCGC 1 cut(s) 111
KasI GGCGCC 1 cut(s) 110
KpnI GGTACC 1 cut(s) 315
KroI GCCGGC 1 cut(s) 359
KroNI GCCGGC 1 cut(s) 361
Kzo9I GATC 2 cut(s) 343, 782
LmnI GCTCC 2 cut(s) 272, 575
Lsp1109I GCAGC 2 cut(s) 282, 625
LweI GCATC 3 cut(s) 313, 346, 785
MaeI CTAG 1 cut(s) 677
MaeII ACGT 5 cut(s) 336, 369, 420, 484, 555
MalI GATC 2 cut(s) 345, 784
MboI GATC 2 cut(s) 343, 782
MboII GAAGA 6 cut(s) 16, 19, 22, 160, 271, 331
MhlI GDGCHC 1 cut(s) 580
MlsI TGGCCA 1 cut(s) 333
MluCI AATT 1 cut(s) 618
MluNI TGGCCA 1 cut(s) 333
Mly113I GGCGCC 1 cut(s) 111
MmeI TCCRAC 2 cut(s) 595, 751
Mox20I TGGCCA 1 cut(s) 333
Mph1103I ATGCAT 1 cut(s) 800
MroNI GCCGGC 1 cut(s) 359
MroXI GAANNNNTTC 2 cut(s) 693, 739
MscI TGGCCA 1 cut(s) 333
MseI TTAA 5 cut(s) 291, 396, 506, 606, 710
MslI CAYNNNNRTG 2 cut(s) 197, 321
Msp20I TGGCCA 1 cut(s) 333
MspI CCGG 3 cut(s) 87, 360, 770
MspR9I CCNGG 4 cut(s) 87, 330, 732, 771
Mva1269I GAATGC 1 cut(s) 798
MvaI CCWGG 2 cut(s) 330, 732
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 1 cut(s) 68
NaeI GCCGGC 1 cut(s) 361
NarI GGCGCC 1 cut(s) 111
NciI CCSGG 2 cut(s) 87, 771
NdeI CATATG 1 cut(s) 643
NdeII GATC 2 cut(s) 343, 782
NgoMIV GCCGGC 1 cut(s) 359
NlaIII CATG 3 cut(s) 202, 259, 320
NlaIV GGNNCC 4 cut(s) 91, 112, 268, 313
NsiI ATGCAT 1 cut(s) 800
NspI RCATGY 1 cut(s) 202
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PctI GAATGC 1 cut(s) 798
PdiI GCCGGC 1 cut(s) 361
PdmI GAANNNNTTC 2 cut(s) 693, 739
PfeI GAWTC 3 cut(s) 130, 165, 655
PkrI GCNGC 3 cut(s) 143, 297, 640
PluTI GGCGCC 1 cut(s) 114
Ppu21I YACGTR 1 cut(s) 556
Psp6I CCWGG 2 cut(s) 328, 730
PspGI CCWGG 2 cut(s) 328, 730
PspN4I GGNNCC 4 cut(s) 91, 112, 268, 313
PspPI GGNCC 1 cut(s) 401
RsaI GTAC 2 cut(s) 313, 483
RsaNI GTAC 2 cut(s) 312, 482
RseI CAYNNNNRTG 2 cut(s) 197, 321
SaqAI TTAA 5 cut(s) 291, 396, 506, 606, 710
SatI GCNGC 3 cut(s) 142, 296, 639
Sau3AI GATC 2 cut(s) 343, 782
Sau96I GGNCC 1 cut(s) 401
ScrFI CCNGG 4 cut(s) 87, 330, 732, 771
SduI GDGCHC 1 cut(s) 580
SfaNI GCATC 3 cut(s) 313, 346, 785
SfcI CTRYAG 2 cut(s) 99, 187
SfoI GGCGCC 1 cut(s) 112
SinI GGWCC 1 cut(s) 401
SmiMI CAYNNNNRTG 2 cut(s) 197, 321
Sse9I AATT 1 cut(s) 618
SsiI CCGC 3 cut(s) 142, 184, 204
SspDI GGCGCC 1 cut(s) 110
SspMI CTAG 1 cut(s) 677
StyD4I CCNGG 4 cut(s) 85, 328, 730, 769
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 1 cut(s) 582
TaiI ACGT 5 cut(s) 339, 372, 423, 487, 558
TaqI TCGA 6 cut(s) 171, 321, 342, 354, 366, 372
TasI AATT 1 cut(s) 618
TauI GCSGC 1 cut(s) 144
TfiI GAWTC 3 cut(s) 130, 165, 655
Tru1I TTAA 5 cut(s) 291, 396, 506, 606, 710
Tru9I TTAA 5 cut(s) 291, 396, 506, 606, 710
TscAI CASTG 5 cut(s) 313, 517, 587, 604, 755
TseI GCWGC 2 cut(s) 295, 638
TspDTI ATGAA 4 cut(s) 17, 272, 470, 581
TspGWI ACGGA 1 cut(s) 648
TspRI CASTG 5 cut(s) 313, 517, 587, 604, 755
VpaK11BI GGWCC 1 cut(s) 401
XbaI TCTAGA 1 cut(s) 676
XceI RCATGY 1 cut(s) 202
XmnI GAANNNNTTC 2 cut(s) 693, 739
XspI CTAG 1 cut(s) 677
ZraI GACGTC 1 cut(s) 370
Zsp2I ATGCAT 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.