Rh6BG071300

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000645
Physical Location & Seq
Reverse (-)
10765714 .. 10767627
1914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG071300.1

Sequence Viewer

Length: 843 bp
ATGGATCACATTCACCATTTTCTTCCTCTTCTGCTGCTACTACCTTGCACGGTGATGTGCACGGCCGTTGCCACAGTCACCTCATCCTCTGACTCAGCTTACCCCATAGTCACCGAGGACTGCTCGGTGGGCTCCGGCGAGGCCGTCCCTGTCCCGGTACGTAGAGAGGTGTACGATAACGGTCGAATATTCGACATCAGCCACAGGTACACCGCTGAAATGCCGAGTTTCGCCTCCGAAAACGGGCGGCTGGGCAGCGACTTCTTGTGGCTGGGATTCAGCATCAAGAACGGATCGCTGGCAAATGCCTCCATGATGAAGTTCTGTGTCCACTCGGGCACTCACGTCGACGCACCTGGTCACGTCTTCGACCATTACTACCATGCTGGATTTGATGTTGATGCTTTAGACCTCGAAGTGTTAAACGGCCCCGCGCTGGTGGTTGATGTTCCCAGGGATAAGAACATAACTGCTGAAGTAATGAAGGCTTTGAACATTCCTAAAGGAGTGCGTCGTGTGCTTTTCAGAACCTTAAATACTGACAGGAAAATTATGTGGAACGTAGCGTACGACACGAGCGCCACAGGATTTACGGAGGATGGGGCAAAATGGTTAGTTCAAAACACAGACATCAAACTCGTTGGAATTGATTACTTAAACGTCGCAGCTTTCGTTGATGTAGTTCCAGTGCATAATACTTTTCTGGAAGGCAGGGAGATAATTCTTGTCGAAGGTTTAAAGCTCGAAGGAGTTCCGGCGGGATTATATTCTTCTGTTCATTGCTTGCCTTTAAGGTTGATTGGTTCCGATGGATCACCAGCAAGATGCATTCTCATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

280

Amino Acids

30.63

Weight (kDa)

5.61

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 64 - 221 1.1e-20 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 348
AccII CGCG 1 cut(s) 434
AciI CCGC 4 cut(s) 213, 247, 432, 758
AclWI GGATC 3 cut(s) 12, 301, 820
AcoI YGGCCR 1 cut(s) 63
AcuI CTGAAG 1 cut(s) 495
AfaI GTAC 4 cut(s) 159, 173, 209, 569
AfiI CCNNNNNNNGG 3 cut(s) 154, 243, 436
AgsI TTSAA 2 cut(s) 493, 620
AjiI CACGTC 2 cut(s) 346, 364
AjnI CCWGG 2 cut(s) 355, 452
AluBI AGCT 3 cut(s) 98, 668, 742
AluI AGCT 3 cut(s) 98, 668, 742
Alw21I GWGCWC 1 cut(s) 62
Alw44I GTGCAC 1 cut(s) 58
AlwI GGATC 3 cut(s) 12, 301, 820
Ama87I CYCGRG 1 cut(s) 334
AoxI GGCC 3 cut(s) 63, 141, 427
ApaLI GTGCAC 1 cut(s) 58
ApeKI GCWGC 3 cut(s) 34, 255, 665
Asp700I GAANNNNTTC 1 cut(s) 750
AspLEI GCGC 2 cut(s) 436, 581
AspS9I GGNCC 1 cut(s) 428
AsuC2I CCSGG 1 cut(s) 155
AsuHPI GGTGA 5 cut(s) 5, 64, 70, 103, 807
AvaI CYCGRG 1 cut(s) 334
BaeGI GKGCMC 2 cut(s) 62, 341
BanII GRGCYC 1 cut(s) 134
BauI CACGAG 1 cut(s) 574
BbsI GAAGAC 1 cut(s) 358
Bbv12I GWGCWC 1 cut(s) 62
BbvI GCAGC 3 cut(s) 21, 267, 677
BccI CCATC 2 cut(s) 593, 803
BceAI ACGGC 4 cut(s) 50, 78, 128, 442
BcgI CGANNNNNNTGC 2 cut(s) 328, 362
BciT130I CCWGG 2 cut(s) 357, 454
BcnI CCSGG 1 cut(s) 155
BfoI RGCGCY 1 cut(s) 582
BisI GCNGC 4 cut(s) 35, 248, 256, 666
BlsI GCNGC 4 cut(s) 36, 249, 257, 667
Bme1390I CCNGG 3 cut(s) 155, 357, 454
BmeT110I CYCGRG 1 cut(s) 334
BmgBI CACGTC 2 cut(s) 346, 364
BmgT120I GGNCC 1 cut(s) 428
BmiI GGNNCC 3 cut(s) 133, 430, 805
BmrFI CCNGG 3 cut(s) 155, 357, 454
BmsI GCATC 3 cut(s) 291, 391, 815
BpiI GAAGAC 1 cut(s) 358
BplI GAGNNNNNCTC 2 cut(s) 107, 139
BpuMI CCSGG 1 cut(s) 155
BsaAI YACGTR 1 cut(s) 161
BsaJI CCNNGG 3 cut(s) 114, 452, 453
Bsc4I CCNNNNNNNGG 3 cut(s) 154, 243, 436
Bse1I ACTGG 1 cut(s) 686
Bse3DI GCAATG 1 cut(s) 778
BseBI CCWGG 2 cut(s) 357, 454
BseDI CCNNGG 3 cut(s) 114, 452, 453
BseGI GGATG 2 cut(s) 83, 604
BseLI CCNNNNNNNGG 3 cut(s) 154, 243, 436
BseMI GCAATG 1 cut(s) 778
BseMII CTCAG 1 cut(s) 108
BseNI ACTGG 1 cut(s) 686
BseSI GKGCMC 2 cut(s) 62, 341
BseX3I CGGCCG 1 cut(s) 63
BseXI GCAGC 3 cut(s) 21, 267, 677
BseYI CCCAGC 2 cut(s) 250, 271
Bsh1236I CGCG 1 cut(s) 434
Bsh1285I CGRYCG 2 cut(s) 66, 184
BshFI GGCC 3 cut(s) 65, 143, 429
BsiEI CGRYCG 2 cut(s) 66, 184
BsiHKAI GWGCWC 1 cut(s) 62
BsiHKCI CYCGRG 1 cut(s) 334
BsiSI CCGG 3 cut(s) 135, 155, 755
BsiWI CGTACG 1 cut(s) 567
BslFI GGGAC 2 cut(s) 131, 137
BslI CCNNNNNNNGG 3 cut(s) 154, 243, 436
BsmFI GGGAC 2 cut(s) 131, 137
BsmI GAATGC 1 cut(s) 828
BsnI GGCC 3 cut(s) 65, 143, 429
BsoBI CYCGRG 1 cut(s) 334
Bsp1286I GDGCHC 3 cut(s) 62, 134, 341
Bsp143I GATC 3 cut(s) 4, 293, 812
BspACI CCGC 4 cut(s) 213, 247, 432, 758
BspANI GGCC 3 cut(s) 65, 143, 429
BspCNI CTCAG 1 cut(s) 107
BspFNI CGCG 1 cut(s) 434
BspLI GGNNCC 3 cut(s) 133, 430, 805
BspPI GGATC 3 cut(s) 12, 301, 820
BsrDI GCAATG 1 cut(s) 778
BsrI ACTGG 1 cut(s) 686
BssECI CCNNGG 3 cut(s) 114, 452, 453
BssMI GATC 3 cut(s) 4, 293, 812
BssSI CACGAG 1 cut(s) 574
Bst2BI CACGAG 1 cut(s) 574
Bst2UI CCWGG 2 cut(s) 357, 454
Bst4CI ACNGT 3 cut(s) 52, 76, 182
Bst6I CTCTTC 1 cut(s) 33
BstBAI YACGTR 1 cut(s) 161
BstC8I GCNNGC 2 cut(s) 300, 785
BstDEI CTNAG 1 cut(s) 94
BstF5I GGATG 2 cut(s) 83, 604
BstFNI CGCG 1 cut(s) 434
BstH2I RGCGCY 1 cut(s) 582
BstHHI GCGC 2 cut(s) 436, 581
BstKTI GATC 3 cut(s) 7, 296, 815
BstMBI GATC 3 cut(s) 4, 293, 812
BstMCI CGRYCG 2 cut(s) 66, 184
BstMWI GCNNNNNNNGC 2 cut(s) 129, 517
BstNI CCWGG 2 cut(s) 357, 454
BstSCI CCNGG 3 cut(s) 153, 355, 452
BstSLI GKGCMC 2 cut(s) 62, 341
BstSNI TACGTA 1 cut(s) 161
BstUI CGCG 1 cut(s) 434
BstV1I GCAGC 3 cut(s) 21, 267, 677
BstV2I GAAGAC 1 cut(s) 358
BstZI CGGCCG 1 cut(s) 63
BsuRI GGCC 3 cut(s) 65, 143, 429
BtrI CACGTC 2 cut(s) 346, 364
BtsCI GGATG 2 cut(s) 83, 604
BtsIMutI CAGTG 1 cut(s) 693
Cac8I GCNNGC 2 cut(s) 300, 785
CfoI GCGC 2 cut(s) 436, 581
Cfr13I GGNCC 1 cut(s) 428
CseI GACGC 2 cut(s) 359, 500
CsiI ACCWGGT 1 cut(s) 355
Csp6I GTAC 4 cut(s) 158, 172, 208, 568
CviAII CATG 2 cut(s) 313, 383
CviQI GTAC 4 cut(s) 158, 172, 208, 568
DdeI CTNAG 1 cut(s) 94
DpnI GATC 3 cut(s) 6, 295, 814
DpnII GATC 3 cut(s) 4, 293, 812
DraI TTTAAA 1 cut(s) 738
EaeI YGGCCR 1 cut(s) 63
EagI CGGCCG 1 cut(s) 63
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
EclXI CGGCCG 1 cut(s) 63
Eco105I TACGTA 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 134
Eco52I CGGCCG 1 cut(s) 63
Eco57I CTGAAG 1 cut(s) 495
Eco88I CYCGRG 1 cut(s) 334
EcoRII CCWGG 2 cut(s) 355, 452
EcoT22I ATGCAT 1 cut(s) 830
EcoT38I GRGCYC 1 cut(s) 134
FaeI CATG 2 cut(s) 316, 386
FaiI YATR 7 cut(s) 107, 314, 384, 467, 554, 693, 766
FaqI GGGAC 2 cut(s) 131, 137
FatI CATG 2 cut(s) 312, 382
FauI CCCGC 2 cut(s) 439, 751
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 4 cut(s) 35, 248, 256, 666
FokI GGATG 2 cut(s) 70, 611
FriOI GRGCYC 1 cut(s) 134
Fsp4HI GCNGC 4 cut(s) 35, 248, 256, 666
GlaI GCGC 2 cut(s) 435, 580
GluI GCNGC 4 cut(s) 35, 248, 256, 666
GsaI CCCAGC 2 cut(s) 254, 275
HaeII RGCGCY 1 cut(s) 582
HaeIII GGCC 3 cut(s) 65, 143, 429
HapII CCGG 3 cut(s) 135, 155, 755
HgaI GACGC 2 cut(s) 359, 500
HhaI GCGC 2 cut(s) 436, 581
Hin1II CATG 2 cut(s) 316, 386
Hin6I GCGC 2 cut(s) 434, 579
HinP1I GCGC 2 cut(s) 434, 579
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HinfI GANTC 2 cut(s) 92, 276
HpaII CCGG 3 cut(s) 135, 155, 755
HphI GGTGA 5 cut(s) 5, 64, 70, 103, 807
Hpy166II GTNNAC 5 cut(s) 60, 172, 210, 331, 349
Hpy188I TCNGA 4 cut(s) 91, 238, 527, 808
Hpy188III TCNNGA 2 cut(s) 286, 704
Hpy8I GTNNAC 5 cut(s) 60, 172, 210, 331, 349
Hpy99I CGWCG 4 cut(s) 350, 353, 516, 665
HpyAV CCTTC 4 cut(s) 478, 701, 725, 740
HpyCH4III ACNGT 3 cut(s) 52, 76, 182
HpyCH4IV ACGT 5 cut(s) 160, 345, 363, 561, 660
HpyCH4V TGCA 4 cut(s) 48, 60, 691, 828
HpyF10VI GCNNNNNNNGC 2 cut(s) 129, 517
HpyF3I CTNAG 1 cut(s) 94
HpySE526I ACGT 5 cut(s) 160, 345, 363, 561, 660
Hsp92II CATG 2 cut(s) 316, 386
HspAI GCGC 2 cut(s) 434, 579
Kzo9I GATC 3 cut(s) 4, 293, 812
LmnI GCTCC 1 cut(s) 137
Lsp1109I GCAGC 3 cut(s) 21, 267, 677
LweI GCATC 3 cut(s) 291, 391, 815
MabI ACCWGGT 1 cut(s) 355
MaeII ACGT 5 cut(s) 160, 345, 363, 561, 660
MaeIII GTNAC 3 cut(s) 76, 109, 359
MalI GATC 3 cut(s) 6, 295, 814
MboI GATC 3 cut(s) 4, 293, 812
MboII GAAGA 4 cut(s) 14, 20, 358, 762
MhlI GDGCHC 3 cut(s) 62, 134, 341
MluCI AATT 3 cut(s) 549, 645, 720
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 1 cut(s) 622
Mph1103I ATGCAT 1 cut(s) 830
MroXI GAANNNNTTC 1 cut(s) 750
MseI TTAA 5 cut(s) 422, 533, 656, 737, 791
MslI CAYNNNNRTG 1 cut(s) 53
MspA1I CMGCKG 1 cut(s) 215
MspI CCGG 3 cut(s) 135, 155, 755
MspR9I CCNGG 3 cut(s) 155, 357, 454
Mva1269I GAATGC 1 cut(s) 828
MvaI CCWGG 2 cut(s) 357, 454
MvnI CGCG 1 cut(s) 434
MwoI GCNNNNNNNGC 2 cut(s) 129, 517
NciI CCSGG 1 cut(s) 155
NdeII GATC 3 cut(s) 4, 293, 812
NlaIII CATG 2 cut(s) 316, 386
NlaIV GGNNCC 3 cut(s) 133, 430, 805
NmeAIII GCCGAG 1 cut(s) 249
NmuCI GTSAC 3 cut(s) 76, 109, 359
NsiI ATGCAT 1 cut(s) 830
PasI CCCWGGG 1 cut(s) 453
PcsI WCGNNNNNNNCGW 2 cut(s) 567, 669
PctI GAATGC 1 cut(s) 828
PdmI GAANNNNTTC 1 cut(s) 750
PfeI GAWTC 1 cut(s) 276
Pfl23II CGTACG 1 cut(s) 567
PkrI GCNGC 4 cut(s) 36, 249, 257, 667
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
Ppu21I YACGTR 1 cut(s) 161
Psp6I CCWGG 2 cut(s) 355, 452
PspFI CCCAGC 2 cut(s) 250, 271
PspGI CCWGG 2 cut(s) 355, 452
PspLI CGTACG 1 cut(s) 567
PspN4I GGNNCC 3 cut(s) 133, 430, 805
PspPI GGNCC 1 cut(s) 428
PsrI GAACNNNNNNTAC 4 cut(s) 520, 551, 552, 583
RsaI GTAC 4 cut(s) 159, 173, 209, 569
RsaNI GTAC 4 cut(s) 158, 172, 208, 568
RseI CAYNNNNRTG 1 cut(s) 53
SalI GTCGAC 1 cut(s) 347
SaqAI TTAA 5 cut(s) 422, 533, 656, 737, 791
SatI GCNGC 4 cut(s) 35, 248, 256, 666
Sau3AI GATC 3 cut(s) 4, 293, 812
Sau96I GGNCC 1 cut(s) 428
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 3 cut(s) 155, 357, 454
SduI GDGCHC 3 cut(s) 62, 134, 341
SexAI ACCWGGT 1 cut(s) 355
SfaNI GCATC 3 cut(s) 291, 391, 815
SgrDI CGTCGACG 1 cut(s) 347
SmiMI CAYNNNNRTG 1 cut(s) 53
SnaBI TACGTA 1 cut(s) 161
Sse9I AATT 3 cut(s) 549, 645, 720
SsiI CCGC 4 cut(s) 213, 247, 432, 758
SspI AATATT 1 cut(s) 189
StyD4I CCNGG 3 cut(s) 153, 355, 452
TaaI ACNGT 3 cut(s) 52, 76, 182
TaiI ACGT 5 cut(s) 163, 348, 366, 564, 663
TaqI TCGA 7 cut(s) 184, 192, 348, 369, 414, 729, 744
TasI AATT 3 cut(s) 549, 645, 720
TauI GCSGC 1 cut(s) 250
TfiI GAWTC 1 cut(s) 276
Tru1I TTAA 5 cut(s) 422, 533, 656, 737, 791
Tru9I TTAA 5 cut(s) 422, 533, 656, 737, 791
TscAI CASTG 1 cut(s) 693
TseFI GTSAC 3 cut(s) 76, 109, 359
TseI GCWGC 3 cut(s) 34, 255, 665
Tsp45I GTSAC 3 cut(s) 76, 109, 359
TspDTI ATGAA 3 cut(s) 332, 497, 767
TspGWI ACGGA 2 cut(s) 306, 608
TspRI CASTG 1 cut(s) 693
VneI GTGCAC 1 cut(s) 58
XmiI GTMKAC 1 cut(s) 348
XmnI GAANNNNTTC 1 cut(s) 750
Zsp2I ATGCAT 1 cut(s) 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.