Rh6BG071200

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000645
Physical Location & Seq
Forward (+)
10762297 .. 10765440
3144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG071200.1

Sequence Viewer

Length: 786 bp
ATGAATCCAATTCTCCTCCTCCTCTTCTTCTGCTCCGTTCCGGCCACCCTCTCCCACGCCACCACAGCCTACCCTTCCATTCCGGGCACCACCCCCGTCGACGTCTCCTCCGGCGCCATCGCCACCAACCTGATCCCTCCTCGCCGTGAAGTCCACGGAAACGGCCGAATCTTCGACATCAGCCACCGCTACACCCCCGACATGCCGGCGTTTGGCTCCGCCGACGGCGTCGGCCAGTTCCTGTGGCTCCCCAACAGCATGAAGAACGGCTCCATTGCTAACAACTCCGAGATGAAGCTCCCCACCCACACCGGGACCCACGTCGACTCCCCCGGCCACGTCTTCGATCACTACTTCGACGCCGGCTTCGACGTCGACACGCTCGACTTGGATGTCCTTAATGGTGCGGCATTGTTAGTTGACGTTCCGAGGGATAAGAACATTACTGCTGAAGTCATGGAGGGCTTACATATTCCCAGGGGCGTGCGTCGTGTACTTTTCAGAACTTTGAACACAGACAGACGGCTTATGTTCCAAAAGGAGTTTGACAGCAGCTATGTGGGATTTATGAAGGATGGAGCTAAGTGGTTGGTGGAGAACACTGACATCAAACTGATTGGAATTGATTACTTATCTGCTGCTGCATATGATGATTTGATTCCGTCTCACCTTGTTTTGCTAGAAGGCAAGGAGATCATTCTTGTAGAAGATGCATTCTTATCAAATGATGGTCTCCTTTATATGCTTGAAATGACTAATGGCCCCAGGATATGTTTCATTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

261

Amino Acids

28.82

Weight (kDa)

5.01

Isoelectric Point (pI)

31.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 58 - 213 3.6e-20 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 392
AatII GACGTC 2 cut(s) 105, 375
AccB1I GGYRCC 2 cut(s) 86, 113
AccI GTMKAC 3 cut(s) 99, 324, 375
AciI CCGC 3 cut(s) 187, 219, 407
AclWI GGATC 1 cut(s) 127
AcoI YGGCCR 4 cut(s) 42, 163, 232, 334
AcuI CTGAAG 1 cut(s) 471
AcyI GRCGYC 5 cut(s) 102, 114, 228, 360, 372
AfaI GTAC 1 cut(s) 495
AfiI CCNNNNNNNGG 2 cut(s) 212, 312
AgsI TTSAA 3 cut(s) 511, 749, 782
AjiI CACGTC 2 cut(s) 322, 340
AjnI CCWGG 2 cut(s) 476, 764
AluBI AGCT 3 cut(s) 298, 555, 581
AluI AGCT 3 cut(s) 298, 555, 581
Alw26I GTCTC 3 cut(s) 109, 669, 737
AlwI GGATC 1 cut(s) 127
AlwNI CAGNNNCTG 1 cut(s) 241
AoxI GGCC 5 cut(s) 42, 163, 232, 334, 760
ApeKI GCWGC 3 cut(s) 552, 638, 641
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 2 cut(s) 315, 761
AsuC2I CCSGG 3 cut(s) 84, 313, 333
AsuHPI GGTGA 1 cut(s) 659
AvaII GGWCC 1 cut(s) 315
BaeGI GKGCMC 1 cut(s) 89
BanI GGYRCC 2 cut(s) 86, 113
BbsI GAAGAC 1 cut(s) 334
BbvI GCAGC 3 cut(s) 564, 625, 628
BccI CCATC 3 cut(s) 125, 569, 722
BceAI ACGGC 5 cut(s) 129, 178, 241, 283, 539
BciT130I CCWGG 2 cut(s) 478, 766
BcnI CCSGG 3 cut(s) 84, 313, 333
BcoDI GTCTC 3 cut(s) 109, 669, 737
BfaI CTAG 1 cut(s) 680
BfoI RGCGCY 1 cut(s) 117
BisI GCNGC 4 cut(s) 408, 553, 639, 642
BlsI GCNGC 4 cut(s) 409, 554, 640, 643
Bme1390I CCNGG 5 cut(s) 84, 313, 333, 478, 766
Bme18I GGWCC 1 cut(s) 315
BmgBI CACGTC 2 cut(s) 322, 340
BmgT120I GGNCC 2 cut(s) 315, 761
BmiI GGNNCC 8 cut(s) 88, 115, 217, 248, 271, 316, 317, 763
BmrFI CCNGG 5 cut(s) 84, 313, 333, 478, 766
BmsI GCATC 1 cut(s) 700
BoxI GACNNNNGTC 1 cut(s) 320
BpiI GAAGAC 1 cut(s) 334
BpuMI CCSGG 3 cut(s) 84, 313, 333
BsaHI GRCGYC 5 cut(s) 102, 114, 228, 360, 372
BsaI GGTCTC 1 cut(s) 737
BsaJI CCNNGG 6 cut(s) 154, 331, 428, 476, 477, 764
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 311, 341
Bsc4I CCNNNNNNNGG 2 cut(s) 212, 312
Bse118I RCCGGY 2 cut(s) 205, 362
Bse1I ACTGG 1 cut(s) 235
Bse3DI GCAATG 1 cut(s) 273
BseBI CCWGG 2 cut(s) 478, 766
BseDI CCNNGG 6 cut(s) 154, 331, 428, 476, 477, 764
BseGI GGATG 2 cut(s) 397, 580
BseLI CCNNNNNNNGG 2 cut(s) 212, 312
BseMI GCAATG 1 cut(s) 273
BseNI ACTGG 1 cut(s) 235
BseRI GAGGAG 5 cut(s) 5, 8, 11, 97, 129
BseSI GKGCMC 1 cut(s) 89
BseX3I CGGCCG 1 cut(s) 163
BseXI GCAGC 3 cut(s) 564, 625, 628
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 5 cut(s) 44, 165, 234, 336, 762
BshNI GGYRCC 2 cut(s) 86, 113
BsiEI CGRYCG 1 cut(s) 166
BsiSI CCGG 7 cut(s) 41, 83, 111, 206, 312, 333, 363
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 2 cut(s) 212, 312
BsmAI GTCTC 3 cut(s) 109, 669, 737
BsmBI CGTCTC 2 cut(s) 109, 669
BsmFI GGGAC 1 cut(s) 328
BsmI GAATGC 1 cut(s) 713
BsnI GGCC 5 cut(s) 44, 165, 234, 336, 762
Bso31I GGTCTC 1 cut(s) 737
Bsp1286I GDGCHC 1 cut(s) 89
Bsp143I GATC 3 cut(s) 132, 346, 693
BspACI CCGC 3 cut(s) 187, 219, 407
BspANI GGCC 5 cut(s) 44, 165, 234, 336, 762
BspLI GGNNCC 8 cut(s) 88, 115, 217, 248, 271, 316, 317, 763
BspPI GGATC 1 cut(s) 127
BspT107I GGYRCC 2 cut(s) 86, 113
BspTNI GGTCTC 1 cut(s) 737
BsrDI GCAATG 1 cut(s) 273
BsrFI RCCGGY 2 cut(s) 205, 362
BsrI ACTGG 1 cut(s) 235
BssAI RCCGGY 2 cut(s) 205, 362
BssECI CCNNGG 6 cut(s) 154, 331, 428, 476, 477, 764
BssMI GATC 3 cut(s) 132, 346, 693
BssNI GRCGYC 5 cut(s) 102, 114, 228, 360, 372
Bst2UI CCWGG 2 cut(s) 478, 766
Bst6I CTCTTC 1 cut(s) 29
BstACI GRCGYC 5 cut(s) 102, 114, 228, 360, 372
BstC8I GCNNGC 3 cut(s) 207, 364, 485
BstDEI CTNAG 1 cut(s) 582
BstDSI CCRYGG 1 cut(s) 154
BstF5I GGATG 2 cut(s) 397, 580
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 3 cut(s) 135, 349, 696
BstMAI GTCTC 3 cut(s) 109, 669, 737
BstMBI GATC 3 cut(s) 132, 346, 693
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 1 cut(s) 65
BstNI CCWGG 2 cut(s) 478, 766
BstNSI RCATGY 1 cut(s) 205
BstPAI GACNNNNGTC 1 cut(s) 320
BstSCI CCNGG 5 cut(s) 82, 311, 331, 476, 764
BstSLI GKGCMC 1 cut(s) 89
BstV1I GCAGC 3 cut(s) 564, 625, 628
BstV2I GAAGAC 1 cut(s) 334
BstZI CGGCCG 1 cut(s) 163
BsuRI GGCC 5 cut(s) 44, 165, 234, 336, 762
BtgI CCRYGG 1 cut(s) 154
BtgZI GCGATG 1 cut(s) 103
BtrI CACGTC 2 cut(s) 322, 340
BtsCI GGATG 2 cut(s) 397, 580
BtsIMutI CAGTG 1 cut(s) 600
Cac8I GCNNGC 3 cut(s) 207, 364, 485
CaiI CAGNNNCTG 1 cut(s) 241
CfoI GCGC 1 cut(s) 116
Cfr10I RCCGGY 2 cut(s) 205, 362
Cfr13I GGNCC 2 cut(s) 315, 761
CseI GACGC 3 cut(s) 217, 368, 476
Csp6I GTAC 1 cut(s) 494
CviAII CATG 3 cut(s) 202, 259, 457
CviQI GTAC 1 cut(s) 494
DdeI CTNAG 1 cut(s) 582
DinI GGCGCC 1 cut(s) 115
DpnI GATC 3 cut(s) 134, 348, 695
DpnII GATC 3 cut(s) 132, 346, 693
DrdI GACNNNNNNGTC 1 cut(s) 392
DseDI GACNNNNNNGTC 1 cut(s) 392
EaeI YGGCCR 4 cut(s) 42, 163, 232, 334
EagI CGGCCG 1 cut(s) 163
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
EciI GGCGGA 1 cut(s) 208
EclXI CGGCCG 1 cut(s) 163
Eco31I GGTCTC 1 cut(s) 737
Eco47I GGWCC 1 cut(s) 315
Eco52I CGGCCG 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 471
EcoO109I RGGNCCY 1 cut(s) 315
EcoRII CCWGG 2 cut(s) 476, 764
EcoT22I ATGCAT 1 cut(s) 715
EgeI GGCGCC 1 cut(s) 115
EheI GGCGCC 1 cut(s) 115
Esp3I CGTCTC 2 cut(s) 109, 669
FaeI CATG 3 cut(s) 205, 262, 460
FaqI GGGAC 1 cut(s) 328
FatI CATG 3 cut(s) 201, 258, 456
FauNDI CATATG 1 cut(s) 646
FblI GTMKAC 3 cut(s) 99, 324, 375
Fnu4HI GCNGC 4 cut(s) 408, 553, 639, 642
FokI GGATG 2 cut(s) 404, 587
Fsp4HI GCNGC 4 cut(s) 408, 553, 639, 642
FspBI CTAG 1 cut(s) 680
GlaI GCGC 1 cut(s) 115
GluI GCNGC 4 cut(s) 408, 553, 639, 642
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 5 cut(s) 44, 165, 234, 336, 762
HapII CCGG 7 cut(s) 41, 83, 111, 206, 312, 333, 363
HgaI GACGC 3 cut(s) 217, 368, 476
HhaI GCGC 1 cut(s) 116
Hin1I GRCGYC 5 cut(s) 102, 114, 228, 360, 372
Hin1II CATG 3 cut(s) 205, 262, 460
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HincII GTYRAC 4 cut(s) 100, 325, 376, 421
HindII GTYRAC 4 cut(s) 100, 325, 376, 421
HinfI GANTC 4 cut(s) 4, 168, 326, 658
HpaII CCGG 7 cut(s) 41, 83, 111, 206, 312, 333, 363
HphI GGTGA 1 cut(s) 659
Hpy166II GTNNAC 6 cut(s) 100, 154, 325, 376, 421, 494
Hpy188I TCNGA 3 cut(s) 289, 429, 503
Hpy8I GTNNAC 6 cut(s) 100, 154, 325, 376, 421, 494
Hpy99I CGWCG 9 cut(s) 101, 104, 227, 233, 326, 362, 374, 377, 492
HpyAV CCTTC 3 cut(s) 84, 565, 677
HpyCH4IV ACGT 5 cut(s) 102, 321, 339, 372, 423
HpyCH4V TGCA 2 cut(s) 644, 713
HpyF10VI GCNNNNNNNGC 1 cut(s) 65
HpyF3I CTNAG 1 cut(s) 582
HpySE526I ACGT 5 cut(s) 102, 321, 339, 372, 423
Hsp92I GRCGYC 5 cut(s) 102, 114, 228, 360, 372
Hsp92II CATG 3 cut(s) 205, 262, 460
HspAI GCGC 1 cut(s) 114
KasI GGCGCC 1 cut(s) 113
KflI GGGWCCC 1 cut(s) 315
KroI GCCGGC 2 cut(s) 205, 362
KroNI GCCGGC 2 cut(s) 207, 364
Kzo9I GATC 3 cut(s) 132, 346, 693
LmnI GCTCC 6 cut(s) 38, 221, 252, 275, 303, 578
Lsp1109I GCAGC 3 cut(s) 564, 625, 628
LweI GCATC 1 cut(s) 700
MaeI CTAG 1 cut(s) 680
MaeII ACGT 5 cut(s) 102, 321, 339, 372, 423
MalI GATC 3 cut(s) 134, 348, 695
MboI GATC 3 cut(s) 132, 346, 693
MboII GAAGA 6 cut(s) 16, 19, 163, 274, 334, 719
MhlI GDGCHC 1 cut(s) 89
MluCI AATT 2 cut(s) 9, 621
Mly113I GGCGCC 1 cut(s) 114
MlyI GAGTC 1 cut(s) 320
MnlI CCTC 9 cut(s) 26, 29, 32, 59, 118, 147, 150, 423, 454
Mph1103I ATGCAT 1 cut(s) 715
MroNI GCCGGC 2 cut(s) 205, 362
MseI TTAA 1 cut(s) 399
MspI CCGG 7 cut(s) 41, 83, 111, 206, 312, 333, 363
MspR9I CCNGG 5 cut(s) 84, 313, 333, 478, 766
Mva1269I GAATGC 1 cut(s) 713
MvaI CCWGG 2 cut(s) 478, 766
MwoI GCNNNNNNNGC 1 cut(s) 65
NaeI GCCGGC 2 cut(s) 207, 364
NarI GGCGCC 1 cut(s) 114
NciI CCSGG 3 cut(s) 84, 313, 333
NdeI CATATG 1 cut(s) 646
NdeII GATC 3 cut(s) 132, 346, 693
NgoMIV GCCGGC 2 cut(s) 205, 362
NlaIII CATG 3 cut(s) 205, 262, 460
NlaIV GGNNCC 8 cut(s) 88, 115, 217, 248, 271, 316, 317, 763
NsiI ATGCAT 1 cut(s) 715
NspI RCATGY 1 cut(s) 205
PasI CCCWGGG 1 cut(s) 477
PcsI WCGNNNNNNNCGW 2 cut(s) 366, 381
PctI GAATGC 1 cut(s) 713
PdiI GCCGGC 2 cut(s) 207, 364
PfeI GAWTC 3 cut(s) 4, 168, 658
PflFI GACNNNGTC 1 cut(s) 227
PkrI GCNGC 4 cut(s) 409, 554, 640, 643
PleI GAGTC 1 cut(s) 320
PluTI GGCGCC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 320
PpuMI RGGWCCY 1 cut(s) 315
PshAI GACNNNNGTC 1 cut(s) 320
Psp5II RGGWCCY 1 cut(s) 315
Psp6I CCWGG 2 cut(s) 476, 764
PspGI CCWGG 2 cut(s) 476, 764
PspN4I GGNNCC 8 cut(s) 88, 115, 217, 248, 271, 316, 317, 763
PspPI GGNCC 2 cut(s) 315, 761
PspPPI RGGWCCY 1 cut(s) 315
PstNI CAGNNNCTG 1 cut(s) 241
PsyI GACNNNGTC 1 cut(s) 227
RsaI GTAC 1 cut(s) 495
RsaNI GTAC 1 cut(s) 494
SalI GTCGAC 3 cut(s) 98, 323, 374
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 4 cut(s) 408, 553, 639, 642
Sau3AI GATC 3 cut(s) 132, 346, 693
Sau96I GGNCC 2 cut(s) 315, 761
SchI GAGTC 1 cut(s) 320
ScrFI CCNGG 5 cut(s) 84, 313, 333, 478, 766
SduI GDGCHC 1 cut(s) 89
SfaNI GCATC 1 cut(s) 700
SfoI GGCGCC 1 cut(s) 115
SgrDI CGTCGACG 1 cut(s) 98
SinI GGWCC 1 cut(s) 315
Sse9I AATT 2 cut(s) 9, 621
SsiI CCGC 3 cut(s) 187, 219, 407
SspDI GGCGCC 1 cut(s) 113
SspMI CTAG 1 cut(s) 680
StyD4I CCNGG 5 cut(s) 82, 311, 331, 476, 764
TaiI ACGT 5 cut(s) 105, 324, 342, 375, 426
TaqI TCGA 8 cut(s) 99, 174, 324, 345, 357, 369, 375, 384
TasI AATT 2 cut(s) 9, 621
TatI WGTACW 1 cut(s) 493
TauI GCSGC 1 cut(s) 410
TfiI GAWTC 3 cut(s) 4, 168, 658
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 1 cut(s) 607
TseI GCWGC 3 cut(s) 552, 638, 641
TspDTI ATGAA 5 cut(s) 17, 275, 308, 584, 766
TspGWI ACGGA 3 cut(s) 25, 171, 651
TspRI CASTG 1 cut(s) 607
Tth111I GACNNNGTC 1 cut(s) 227
VpaK11BI GGWCC 1 cut(s) 315
XceI RCATGY 1 cut(s) 205
XmiI GTMKAC 3 cut(s) 99, 324, 375
XspI CTAG 1 cut(s) 680
ZraI GACGTC 2 cut(s) 103, 373
Zsp2I ATGCAT 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.