Rh6DG066800

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000645
Physical Location & Seq
Forward (+)
6811251 .. 6815057
3807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG066800.1

Sequence Viewer

Length: 813 bp
ATGAATCCAATCCTTCTCCTTCTCCTCTTCTCCCTTCAGGCCACCCTCTCCCACGCCACCACAGCCTACCCTTCCATTCCGGGCACCACCCCCGTCGACGTCTCCTCCGGCGCCACCGCCACCAACCTGATCCCCCCTCGCCGCGAAGTCCACGGAAACGGCCGAATCTTCGACATCAGCCACCGCTACAGCCCCGACATGCCGGCGTTTGACTCCGCCGACGGCGTCGGCCAGTTCCTCTGGCTCCCCAACAGCATGAAGAACGGCTCCATTGCTAACAACTCCGAGATGAAGCTCCCCACCCACACCGGGACCCACGTCGACGCCCCCGGCCACGTCTTCGATCACTACTTCGACGCCGGCTTCGACGTCGACACGCTCGACTTGGATGTCCTTAATGGTGCGGCATTGTTAGTTGACGTTCCGAGGGATAAGAACATTACTGCTGAAGTCATGGAGGGCTTACATATTCCCAAGGGGGTGCGTCGTGTACTTTTCAGAACTTTGAACACAGACAGACGGCTTATGTTCCAAAAGGAGTTTGACAGCAGCTATGTGGGATTTATGAAGGATGGAGCTAAGTGGTTGGTGGAGAACACTGACATCAAACTGATTGGAATTGATTACTTATCTGCTGCTGCATATGATGATTTGATTCCGTCTCACCTTGTTCTGCTAGAAGGCAGGGAGATCATTCTTGTAGAAGGTCTAAAACTTGATGACATCCAACCAGGAATATATTCTGTCCATTGCTTACCTTTGAGGCTGACAGGTGCAGAAGGATCACCAATCAGATGCATTCTTATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

270

Amino Acids

29.67

Weight (kDa)

5.39

Isoelectric Point (pI)

33.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 57 - 212 2.9e-21 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44542 AT4G34180 AT4G35220
fragaria_vesca FvH4_2g05360 FvH4_2g05360 FvH4_2g05380 FvH4_2g05400
malus_domestica MD00G1024900.v1.1 MD05G1087600.v1.1
prunus_persica Prupe.8G132800_v2.0.a1 Prupe.8G132800_v2.0.a1 Prupe.8G132900_v2.0.a1 Prupe.8G133000_v2.0.a1 Prupe.8G133300_v2.0.a1 Prupe.8G133400_v2.0.a1
pyrus_communis pycom05g08710
rosa_chinensis RchiOBHm_Chr6g0255621 RchiOBHm_Chr6g0255631 RchiOBHm_Chr6g0255661 RchiOBHm_Chr6g0255671 RchiOBHm_Chr6g0255681 RchiOBHm_Chr6g0255711
rosa_laevigata RLG00000014820 RLG00000014821 RLG00000014822 RLG00000014826
rosa_multiflora Rmu_co8458743.1_g000001 Rmu_sc0001422.1_g000004 Rmu_sc0005014.1_g000014 Rmu_sc0010421.1_g000001 Rmu_sc0010421.1_g000005 Rmu_sc0030046.1_g000001 Rmu_sc0039760.1_g000001 Rmu_ssc0000136.1_g000001 Rmu_ssc0000136.1_g000015
rosa_roxburghii Rroxscaffold_7G00209750 Rroxscaffold_7G00209760 Rroxscaffold_7G00209780 Rroxscaffold_7G00209790 Rroxscaffold_7G00209800 Rroxscaffold_7G00209810 Rroxscaffold_7G00209820 Rroxscaffold_7G00209830 Rroxscaffold_7G00209840
rosa_rugosa Rorug05G0559900 Rorug05G0560000 Rorug05G0560200 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560300 Rorug05G0560400 Rorug05G0560500
rosa_samantha Rh4BG250600 Rh6AG078600 Rh6AG078700 Rh6BG070800 Rh6BG071000 Rh6BG071200 Rh6BG071300 Rh6BG071600 Rh6BG071700 Rh6CG069300 Rh6CG069400 Rh6CG069700 Rh6CG069800 Rh6CG069900 Rh6DG066300 Rh6DG066400 Rh6DG066800 Rh6DG066900 Rh6DG067100 Rh6DG372200
rosa_wichuraiana Rw2G024460 Rw6G006610 Rw6G006770 Rw6G006780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 389
AatII GACGTC 2 cut(s) 102, 372
AccB1I GGYRCC 2 cut(s) 83, 110
AccI GTMKAC 3 cut(s) 96, 321, 372
AccII CGCG 1 cut(s) 144
AciI CCGC 5 cut(s) 117, 142, 184, 216, 404
AclWI GGATC 2 cut(s) 124, 790
AcoI YGGCCR 3 cut(s) 160, 229, 331
AcuI CTGAAG 2 cut(s) 20, 468
AcyI GRCGYC 6 cut(s) 99, 111, 225, 324, 357, 369
AfaI GTAC 1 cut(s) 492
AfiI CCNNNNNNNGG 1 cut(s) 309
AgsI TTSAA 1 cut(s) 508
AjiI CACGTC 2 cut(s) 319, 337
AjnI CCWGG 1 cut(s) 730
AluBI AGCT 3 cut(s) 295, 552, 578
AluI AGCT 3 cut(s) 295, 552, 578
Alw26I GTCTC 2 cut(s) 106, 666
AlwI GGATC 2 cut(s) 124, 790
AoxI GGCC 4 cut(s) 39, 160, 229, 331
ApeKI GCWGC 3 cut(s) 549, 635, 638
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
Asp700I GAANNNNTTC 1 cut(s) 739
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 1 cut(s) 312
AsuC2I CCSGG 3 cut(s) 81, 310, 330
AsuHPI GGTGA 2 cut(s) 656, 777
AvaII GGWCC 1 cut(s) 312
BaeGI GKGCMC 1 cut(s) 86
BanI GGYRCC 2 cut(s) 83, 110
BbsI GAAGAC 1 cut(s) 331
BbvI GCAGC 3 cut(s) 561, 622, 625
BccI CCATC 1 cut(s) 566
BceAI ACGGC 4 cut(s) 175, 238, 280, 536
BciT130I CCWGG 1 cut(s) 732
BcnI CCSGG 3 cut(s) 81, 310, 330
BcoDI GTCTC 2 cut(s) 106, 666
BfaI CTAG 1 cut(s) 677
BfmI CTRYAG 1 cut(s) 187
BfoI RGCGCY 1 cut(s) 114
BisI GCNGC 5 cut(s) 142, 405, 550, 636, 639
BlsI GCNGC 5 cut(s) 143, 406, 551, 637, 640
Bme1390I CCNGG 4 cut(s) 81, 310, 330, 732
Bme18I GGWCC 1 cut(s) 312
BmgBI CACGTC 2 cut(s) 319, 337
BmgT120I GGNCC 1 cut(s) 312
BmiI GGNNCC 6 cut(s) 85, 112, 245, 268, 313, 314
BmrFI CCNGG 4 cut(s) 81, 310, 330, 732
BmsI GCATC 1 cut(s) 785
BoxI GACNNNNGTC 1 cut(s) 317
BpiI GAAGAC 1 cut(s) 331
BpuMI CCSGG 3 cut(s) 81, 310, 330
BsaHI GRCGYC 6 cut(s) 99, 111, 225, 324, 357, 369
BsaJI CCNNGG 4 cut(s) 151, 328, 425, 474
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 1 cut(s) 309
Bse118I RCCGGY 2 cut(s) 202, 359
Bse1I ACTGG 1 cut(s) 232
Bse3DI GCAATG 2 cut(s) 270, 748
BseBI CCWGG 1 cut(s) 732
BseDI CCNNGG 4 cut(s) 151, 328, 425, 474
BseGI GGATG 3 cut(s) 394, 577, 723
BseLI CCNNNNNNNGG 1 cut(s) 309
BseMI GCAATG 2 cut(s) 270, 748
BseNI ACTGG 1 cut(s) 232
BseRI GAGGAG 2 cut(s) 14, 94
BseSI GKGCMC 1 cut(s) 86
BseX3I CGGCCG 1 cut(s) 160
BseXI GCAGC 3 cut(s) 561, 622, 625
BsgI GTGCAG 1 cut(s) 795
Bsh1236I CGCG 1 cut(s) 144
Bsh1285I CGRYCG 1 cut(s) 163
BshFI GGCC 4 cut(s) 41, 162, 231, 333
BshNI GGYRCC 2 cut(s) 83, 110
BsiEI CGRYCG 1 cut(s) 163
BsiSI CCGG 6 cut(s) 80, 108, 203, 309, 330, 360
BslFI GGGAC 1 cut(s) 325
BslI CCNNNNNNNGG 1 cut(s) 309
BsmAI GTCTC 2 cut(s) 106, 666
BsmBI CGTCTC 2 cut(s) 106, 666
BsmFI GGGAC 1 cut(s) 325
BsmI GAATGC 1 cut(s) 798
BsnI GGCC 4 cut(s) 41, 162, 231, 333
Bsp1286I GDGCHC 1 cut(s) 86
Bsp143I GATC 4 cut(s) 129, 343, 690, 782
BspACI CCGC 5 cut(s) 117, 142, 184, 216, 404
BspANI GGCC 4 cut(s) 41, 162, 231, 333
BspFNI CGCG 1 cut(s) 144
BspLI GGNNCC 6 cut(s) 85, 112, 245, 268, 313, 314
BspPI GGATC 2 cut(s) 124, 790
BspT107I GGYRCC 2 cut(s) 83, 110
BsrDI GCAATG 2 cut(s) 270, 748
BsrFI RCCGGY 2 cut(s) 202, 359
BsrI ACTGG 1 cut(s) 232
BssAI RCCGGY 2 cut(s) 202, 359
BssECI CCNNGG 4 cut(s) 151, 328, 425, 474
BssMI GATC 4 cut(s) 129, 343, 690, 782
BssNI GRCGYC 6 cut(s) 99, 111, 225, 324, 357, 369
BssT1I CCWWGG 1 cut(s) 474
Bst2UI CCWGG 1 cut(s) 732
Bst6I CTCTTC 1 cut(s) 32
BstACI GRCGYC 6 cut(s) 99, 111, 225, 324, 357, 369
BstC8I GCNNGC 2 cut(s) 204, 361
BstDEI CTNAG 1 cut(s) 579
BstDSI CCRYGG 1 cut(s) 151
BstF5I GGATG 3 cut(s) 394, 577, 723
BstFNI CGCG 1 cut(s) 144
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 1 cut(s) 113
BstKTI GATC 4 cut(s) 132, 346, 693, 785
BstMAI GTCTC 2 cut(s) 106, 666
BstMBI GATC 4 cut(s) 129, 343, 690, 782
BstMCI CGRYCG 1 cut(s) 163
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNI CCWGG 1 cut(s) 732
BstNSI RCATGY 1 cut(s) 202
BstPAI GACNNNNGTC 1 cut(s) 317
BstSCI CCNGG 4 cut(s) 79, 308, 328, 730
BstSFI CTRYAG 1 cut(s) 187
BstSLI GKGCMC 1 cut(s) 86
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 3 cut(s) 561, 622, 625
BstV2I GAAGAC 1 cut(s) 331
BstZI CGGCCG 1 cut(s) 160
BsuRI GGCC 4 cut(s) 41, 162, 231, 333
BtgI CCRYGG 1 cut(s) 151
BtrI CACGTC 2 cut(s) 319, 337
BtsCI GGATG 3 cut(s) 394, 577, 723
BtsIMutI CAGTG 1 cut(s) 597
Cac8I GCNNGC 2 cut(s) 204, 361
CfoI GCGC 1 cut(s) 113
Cfr10I RCCGGY 2 cut(s) 202, 359
Cfr13I GGNCC 1 cut(s) 312
CseI GACGC 4 cut(s) 214, 332, 365, 473
Csp6I GTAC 1 cut(s) 491
CviAII CATG 3 cut(s) 199, 256, 454
CviQI GTAC 1 cut(s) 491
DdeI CTNAG 1 cut(s) 579
DinI GGCGCC 1 cut(s) 112
DpnI GATC 4 cut(s) 131, 345, 692, 784
DpnII GATC 4 cut(s) 129, 343, 690, 782
DrdI GACNNNNNNGTC 1 cut(s) 389
DseDI GACNNNNNNGTC 1 cut(s) 389
EaeI YGGCCR 3 cut(s) 160, 229, 331
EagI CGGCCG 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EciI GGCGGA 1 cut(s) 205
EclXI CGGCCG 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 474
Eco47I GGWCC 1 cut(s) 312
Eco52I CGGCCG 1 cut(s) 160
Eco57I CTGAAG 2 cut(s) 20, 468
EcoO109I RGGNCCY 1 cut(s) 312
EcoRII CCWGG 1 cut(s) 730
EcoT14I CCWWGG 1 cut(s) 474
EcoT22I ATGCAT 1 cut(s) 800
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 474
Esp3I CGTCTC 2 cut(s) 106, 666
FaeI CATG 3 cut(s) 202, 259, 457
FaqI GGGAC 1 cut(s) 325
FatI CATG 3 cut(s) 198, 255, 453
FauNDI CATATG 1 cut(s) 643
FblI GTMKAC 3 cut(s) 96, 321, 372
Fnu4HI GCNGC 5 cut(s) 142, 405, 550, 636, 639
FokI GGATG 3 cut(s) 401, 584, 710
Fsp4HI GCNGC 5 cut(s) 142, 405, 550, 636, 639
FspBI CTAG 1 cut(s) 677
GlaI GCGC 1 cut(s) 112
GluI GCNGC 5 cut(s) 142, 405, 550, 636, 639
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 4 cut(s) 41, 162, 231, 333
HapII CCGG 6 cut(s) 80, 108, 203, 309, 330, 360
HgaI GACGC 4 cut(s) 214, 332, 365, 473
HhaI GCGC 1 cut(s) 113
Hin1I GRCGYC 6 cut(s) 99, 111, 225, 324, 357, 369
Hin1II CATG 3 cut(s) 202, 259, 457
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HincII GTYRAC 4 cut(s) 97, 322, 373, 418
HindII GTYRAC 4 cut(s) 97, 322, 373, 418
HinfI GANTC 4 cut(s) 4, 165, 212, 655
HpaII CCGG 6 cut(s) 80, 108, 203, 309, 330, 360
HphI GGTGA 2 cut(s) 656, 777
Hpy166II GTNNAC 6 cut(s) 97, 151, 322, 373, 418, 491
Hpy188I TCNGA 4 cut(s) 286, 426, 500, 794
Hpy8I GTNNAC 6 cut(s) 97, 151, 322, 373, 418, 491
HpyAV CCTTC 8 cut(s) 23, 29, 44, 81, 562, 674, 698, 773
HpyCH4IV ACGT 5 cut(s) 99, 318, 336, 369, 420
HpyCH4V TGCA 3 cut(s) 641, 776, 798
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 1 cut(s) 579
HpySE526I ACGT 5 cut(s) 99, 318, 336, 369, 420
Hsp92I GRCGYC 6 cut(s) 99, 111, 225, 324, 357, 369
Hsp92II CATG 3 cut(s) 202, 259, 457
HspAI GCGC 1 cut(s) 111
KasI GGCGCC 1 cut(s) 110
KflI GGGWCCC 1 cut(s) 312
KroI GCCGGC 2 cut(s) 202, 359
KroNI GCCGGC 2 cut(s) 204, 361
Kzo9I GATC 4 cut(s) 129, 343, 690, 782
LmnI GCTCC 4 cut(s) 249, 272, 300, 575
Lsp1109I GCAGC 3 cut(s) 561, 622, 625
LweI GCATC 1 cut(s) 785
MaeI CTAG 1 cut(s) 677
MaeII ACGT 5 cut(s) 99, 318, 336, 369, 420
MalI GATC 4 cut(s) 131, 345, 692, 784
MboI GATC 4 cut(s) 129, 343, 690, 782
MboII GAAGA 4 cut(s) 19, 160, 271, 331
MhlI GDGCHC 1 cut(s) 86
MluCI AATT 1 cut(s) 618
Mly113I GGCGCC 1 cut(s) 111
MlyI GAGTC 1 cut(s) 206
MmeI TCCRAC 1 cut(s) 751
MnlI CCTC 8 cut(s) 35, 56, 115, 147, 248, 420, 451, 756
Mph1103I ATGCAT 1 cut(s) 800
MroNI GCCGGC 2 cut(s) 202, 359
MroXI GAANNNNTTC 1 cut(s) 739
MseI TTAA 1 cut(s) 396
MspI CCGG 6 cut(s) 80, 108, 203, 309, 330, 360
MspR9I CCNGG 4 cut(s) 81, 310, 330, 732
Mva1269I GAATGC 1 cut(s) 798
MvaI CCWGG 1 cut(s) 732
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 1 cut(s) 62
NaeI GCCGGC 2 cut(s) 204, 361
NarI GGCGCC 1 cut(s) 111
NciI CCSGG 3 cut(s) 81, 310, 330
NdeI CATATG 1 cut(s) 643
NdeII GATC 4 cut(s) 129, 343, 690, 782
NgoMIV GCCGGC 2 cut(s) 202, 359
NlaIII CATG 3 cut(s) 202, 259, 457
NlaIV GGNNCC 6 cut(s) 85, 112, 245, 268, 313, 314
NsiI ATGCAT 1 cut(s) 800
NspI RCATGY 1 cut(s) 202
PcsI WCGNNNNNNNCGW 2 cut(s) 363, 378
PctI GAATGC 1 cut(s) 798
PdiI GCCGGC 2 cut(s) 204, 361
PdmI GAANNNNTTC 1 cut(s) 739
PfeI GAWTC 3 cut(s) 4, 165, 655
PflFI GACNNNGTC 1 cut(s) 224
PkrI GCNGC 5 cut(s) 143, 406, 551, 637, 640
PleI GAGTC 1 cut(s) 206
PluTI GGCGCC 1 cut(s) 114
PpsI GAGTC 1 cut(s) 206
PpuMI RGGWCCY 1 cut(s) 312
PshAI GACNNNNGTC 1 cut(s) 317
Psp5II RGGWCCY 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 730
PspGI CCWGG 1 cut(s) 730
PspN4I GGNNCC 6 cut(s) 85, 112, 245, 268, 313, 314
PspPI GGNCC 1 cut(s) 312
PspPPI RGGWCCY 1 cut(s) 312
PsyI GACNNNGTC 1 cut(s) 224
RsaI GTAC 1 cut(s) 492
RsaNI GTAC 1 cut(s) 491
SalI GTCGAC 3 cut(s) 95, 320, 371
SaqAI TTAA 1 cut(s) 396
SatI GCNGC 5 cut(s) 142, 405, 550, 636, 639
Sau3AI GATC 4 cut(s) 129, 343, 690, 782
Sau96I GGNCC 1 cut(s) 312
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 4 cut(s) 81, 310, 330, 732
SduI GDGCHC 1 cut(s) 86
SfaNI GCATC 1 cut(s) 785
SfcI CTRYAG 1 cut(s) 187
SfoI GGCGCC 1 cut(s) 112
SgrDI CGTCGACG 2 cut(s) 95, 320
SinI GGWCC 1 cut(s) 312
Sse9I AATT 1 cut(s) 618
SsiI CCGC 5 cut(s) 117, 142, 184, 216, 404
SspDI GGCGCC 1 cut(s) 110
SspMI CTAG 1 cut(s) 677
StyD4I CCNGG 4 cut(s) 79, 308, 328, 730
StyI CCWWGG 1 cut(s) 474
TaiI ACGT 5 cut(s) 102, 321, 339, 372, 423
TaqI TCGA 8 cut(s) 96, 171, 321, 342, 354, 366, 372, 381
TasI AATT 1 cut(s) 618
TatI WGTACW 1 cut(s) 490
TauI GCSGC 2 cut(s) 144, 407
TfiI GAWTC 3 cut(s) 4, 165, 655
Tru1I TTAA 1 cut(s) 396
Tru9I TTAA 1 cut(s) 396
TscAI CASTG 1 cut(s) 604
TseI GCWGC 3 cut(s) 549, 635, 638
TspDTI ATGAA 4 cut(s) 17, 272, 305, 581
TspGWI ACGGA 2 cut(s) 168, 648
TspRI CASTG 1 cut(s) 604
Tth111I GACNNNGTC 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 312
XceI RCATGY 1 cut(s) 202
XmiI GTMKAC 3 cut(s) 96, 321, 372
XmnI GAANNNNTTC 1 cut(s) 739
XspI CTAG 1 cut(s) 677
ZraI GACGTC 2 cut(s) 100, 370
Zsp2I ATGCAT 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.