Rorug03G0333900

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000696
Physical Location & Seq
Reverse (-)
39145485 .. 39149487
4003 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0333900.1

Sequence Viewer

Length: 825 bp
ATGGCTACTAACGAAAATCTGCCACCAAACGTGATCAAGCAACTTGCAAAGGAATTGAAGAGTCTTGACGAATCCCCTCCTGAAGGCATTAAAGTTTGCGTGAATGACGACGACTTTTCAATCATATTTGCTGATATTGAAGGGCCAGCTGGCACTCCTTATGAGAATGGTGTTTTTCGCATGAAGTTGTTGTTATCTCATGACTTTCCACACTCCCCCCCCAAAGGTTACTTTCTGACTAGGATTTTCCATCCAAACATTGCAAATAATGGTGAGATTTGTGTGAACACGTTAAAAAAAGATTGGAATCCAAGTCTTGGATTACGACATGTTCTCATTGTGGTTAGATGTCTGTTGATTGAACCATTTCCAGAATCAGCCTTAAATGAACAAGCAGGGAAGATGCTGCTTGAGAATTATGAGGAATATGCAAGACATGCCAGGCTTTATACCGGAATACATGCCAAGCCAAAACCCAAGTTTAAAACAGGAGCTATTTCTGAGTCTACTACTGCACTGAATGTTGACCAATCTAACACCTCAGCACTTAATGCTGATCAAAAGATCACGGCAGGTGGTGCTGCTTTGCCACTGCCATCCCCATCCCCATCCCCATCCGTACTGGTCCCTTTGACAGTCACTGCAAGAGGACTTGGCCAGGATCAGCCGGCTGTTGTAGCTGCTGCAGCGGAGACGAGAGTTGGTGGGGCTGCTGCATCAACTGTAGCAAGTAGTACGACACTGAAGAAGGAAGCTGGCTTGATAAAAGCACAGGCAGAAAAGAAGAAAATCGATGCAAGAAAGAAAAGTCTGAAGAGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

274

Amino Acids

29.45

Weight (kDa)

8.99

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 15 - 150 1.1e-38 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 563
Acc36I ACCTGC 1 cut(s) 563
AccB7I CCANNNNNTGG 1 cut(s) 317
AccI GTMKAC 1 cut(s) 506
AciI CCGC 1 cut(s) 689
AclWI GGATC 1 cut(s) 669
AcoI YGGCCR 1 cut(s) 655
AcuI CTGAAG 2 cut(s) 102, 764
AfaI GTAC 2 cut(s) 621, 736
AfiI CCNNNNNNNGG 3 cut(s) 83, 224, 317
AflIII ACRYGT 2 cut(s) 288, 328
AgsI TTSAA 4 cut(s) 58, 120, 140, 362
AjnI CCWGG 2 cut(s) 440, 657
AluBI AGCT 4 cut(s) 149, 494, 680, 755
AluI AGCT 4 cut(s) 149, 494, 680, 755
Alw26I GTCTC 1 cut(s) 686
AlwI GGATC 1 cut(s) 669
AlwNI CAGNNNCTG 1 cut(s) 641
AoxI GGCC 2 cut(s) 143, 655
ApeKI GCWGC 7 cut(s) 406, 581, 680, 683, 686, 710, 713
Asp700I GAANNNNTTC 1 cut(s) 366
AspS9I GGNCC 2 cut(s) 143, 625
AsuHPI GGTGA 1 cut(s) 284
AvaII GGWCC 1 cut(s) 625
BalI TGGCCA 1 cut(s) 657
BbvCI CCTCAGC 1 cut(s) 541
BbvI GCAGC 7 cut(s) 393, 568, 667, 670, 697, 698, 700
BccI CCATC 5 cut(s) 258, 604, 610, 616, 622
BceAI ACGGC 1 cut(s) 585
BciT130I CCWGG 2 cut(s) 442, 659
BclI TGATCA 2 cut(s) 33, 556
BcoDI GTCTC 1 cut(s) 686
BfaI CTAG 1 cut(s) 240
BfmI CTRYAG 2 cut(s) 684, 723
BfuAI ACCTGC 1 cut(s) 563
BisI GCNGC 7 cut(s) 407, 582, 681, 684, 687, 711, 714
BlsI GCNGC 7 cut(s) 408, 583, 682, 685, 688, 712, 715
Bme1390I CCNGG 2 cut(s) 442, 659
Bme18I GGWCC 1 cut(s) 625
BmgT120I GGNCC 2 cut(s) 143, 625
BmiI GGNNCC 1 cut(s) 627
BmrFI CCNGG 2 cut(s) 442, 659
BmsI GCATC 3 cut(s) 393, 725, 784
Bpu10I CCTNAGC 1 cut(s) 541
BpuEI CTTGAG 1 cut(s) 431
Bsa29I ATCGAT 1 cut(s) 792
BsaBI GATNNNNATC 1 cut(s) 306
BsaWI WCCGGW 1 cut(s) 452
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 224, 317
Bse118I RCCGGY 1 cut(s) 667
Bse1I ACTGG 1 cut(s) 627
Bse3DI GCAATG 1 cut(s) 258
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 2 cut(s) 442, 659
BseCI ATCGAT 1 cut(s) 792
BseGI GGATG 5 cut(s) 250, 596, 602, 608, 614
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 3 cut(s) 83, 224, 317
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 2 cut(s) 492, 555
BseNI ACTGG 1 cut(s) 627
BseXI GCAGC 7 cut(s) 393, 568, 667, 670, 697, 698, 700
BsgI GTGCAG 1 cut(s) 498
BshFI GGCC 2 cut(s) 145, 657
BshVI ATCGAT 1 cut(s) 792
BsiSI CCGG 2 cut(s) 453, 668
BslFI GGGAC 1 cut(s) 611
BslI CCNNNNNNNGG 3 cut(s) 83, 224, 317
BsmAI GTCTC 1 cut(s) 686
BsmBI CGTCTC 1 cut(s) 686
BsmFI GGGAC 1 cut(s) 611
BsnI GGCC 2 cut(s) 145, 657
Bsp143I GATC 4 cut(s) 33, 556, 564, 661
BspACI CCGC 1 cut(s) 689
BspANI GGCC 2 cut(s) 145, 657
BspCNI CTCAG 2 cut(s) 493, 554
BspDI ATCGAT 1 cut(s) 792
BspHI TCATGA 1 cut(s) 199
BspLI GGNNCC 1 cut(s) 627
BspMAI CTGCAG 1 cut(s) 688
BspMI ACCTGC 1 cut(s) 563
BspPI GGATC 1 cut(s) 669
BsrDI GCAATG 1 cut(s) 258
BsrFI RCCGGY 1 cut(s) 667
BsrI ACTGG 1 cut(s) 627
BssAI RCCGGY 1 cut(s) 667
BssMI GATC 4 cut(s) 33, 556, 564, 661
Bst2UI CCWGG 2 cut(s) 442, 659
Bst4CI ACNGT 2 cut(s) 637, 724
Bst6I CTCTTC 2 cut(s) 53, 809
BstAPI GCANNNNNTGC 3 cut(s) 437, 551, 578
BstC8I GCNNGC 4 cut(s) 147, 151, 669, 757
BstDEI CTNAG 2 cut(s) 501, 541
BstENI CCTNNNNNAGG 1 cut(s) 81
BstF5I GGATG 5 cut(s) 250, 596, 602, 608, 614
BstKTI GATC 4 cut(s) 36, 559, 567, 664
BstMAI GTCTC 1 cut(s) 686
BstMBI GATC 4 cut(s) 33, 556, 564, 661
BstMWI GCNNNNNNNGC 5 cut(s) 437, 551, 578, 677, 686
BstNI CCWGG 2 cut(s) 442, 659
BstNSI RCATGY 3 cut(s) 332, 440, 464
BstSCI CCNGG 2 cut(s) 440, 657
BstSFI CTRYAG 2 cut(s) 684, 723
BstV1I GCAGC 7 cut(s) 393, 568, 667, 670, 697, 698, 700
Bsu15I ATCGAT 1 cut(s) 792
BsuRI GGCC 2 cut(s) 145, 657
BsuTUI ATCGAT 1 cut(s) 792
BtsCI GGATG 5 cut(s) 250, 596, 602, 608, 614
BtsI GCAGTG 2 cut(s) 590, 639
BtsIMutI CAGTG 4 cut(s) 515, 590, 639, 740
BveI ACCTGC 1 cut(s) 563
Cac8I GCNNGC 4 cut(s) 147, 151, 669, 757
CaiI CAGNNNCTG 1 cut(s) 641
CciI TCATGA 1 cut(s) 199
Cfr10I RCCGGY 1 cut(s) 667
Cfr13I GGNCC 2 cut(s) 143, 625
ClaI ATCGAT 1 cut(s) 792
Csp6I GTAC 2 cut(s) 620, 735
CviAII CATG 5 cut(s) 181, 200, 329, 437, 461
CviQI GTAC 2 cut(s) 620, 735
DdeI CTNAG 2 cut(s) 501, 541
DpnI GATC 4 cut(s) 35, 558, 566, 663
DpnII GATC 4 cut(s) 33, 556, 564, 661
DraI TTTAAA 1 cut(s) 484
EaeI YGGCCR 1 cut(s) 655
Eam1104I CTCTTC 2 cut(s) 53, 809
EarI CTCTTC 2 cut(s) 53, 809
Eco47I GGWCC 1 cut(s) 625
Eco57I CTGAAG 2 cut(s) 102, 764
EcoNI CCTNNNNNAGG 1 cut(s) 81
EcoRII CCWGG 2 cut(s) 440, 657
Esp3I CGTCTC 1 cut(s) 686
FaeI CATG 5 cut(s) 184, 203, 332, 440, 464
FaqI GGGAC 1 cut(s) 611
FatI CATG 5 cut(s) 180, 199, 328, 436, 460
FbaI TGATCA 2 cut(s) 33, 556
FblI GTMKAC 1 cut(s) 506
Fnu4HI GCNGC 7 cut(s) 407, 582, 681, 684, 687, 711, 714
FokI GGATG 5 cut(s) 237, 583, 589, 595, 601
Fsp4HI GCNGC 7 cut(s) 407, 582, 681, 684, 687, 711, 714
FspBI CTAG 1 cut(s) 240
GluI GCNGC 7 cut(s) 407, 582, 681, 684, 687, 711, 714
HaeIII GGCC 2 cut(s) 145, 657
HapII CCGG 2 cut(s) 453, 668
Hin1II CATG 5 cut(s) 184, 203, 332, 440, 464
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HinfI GANTC 5 cut(s) 61, 71, 307, 374, 503
HpaII CCGG 2 cut(s) 453, 668
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 3 cut(s) 286, 507, 526
Hpy188I TCNGA 3 cut(s) 237, 502, 813
Hpy188III TCNNGA 4 cut(s) 65, 80, 200, 371
Hpy8I GTNNAC 3 cut(s) 286, 507, 526
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 3 cut(s) 77, 134, 742
HpyCH4III ACNGT 2 cut(s) 637, 724
HpyCH4IV ACGT 2 cut(s) 30, 290
HpyCH4V TGCA 8 cut(s) 47, 263, 431, 515, 644, 686, 716, 797
HpyF10VI GCNNNNNNNGC 5 cut(s) 437, 551, 578, 677, 686
HpyF3I CTNAG 2 cut(s) 501, 541
HpySE526I ACGT 2 cut(s) 30, 290
Hsp92II CATG 5 cut(s) 184, 203, 332, 440, 464
KroI GCCGGC 1 cut(s) 667
KroNI GCCGGC 1 cut(s) 669
Ksp22I TGATCA 2 cut(s) 33, 556
Kzo9I GATC 4 cut(s) 33, 556, 564, 661
LmnI GCTCC 1 cut(s) 491
Lsp1109I GCAGC 7 cut(s) 393, 568, 667, 670, 697, 698, 700
LweI GCATC 3 cut(s) 393, 725, 784
MaeI CTAG 1 cut(s) 240
MaeII ACGT 2 cut(s) 30, 290
MaeIII GTNAC 2 cut(s) 227, 637
MalI GATC 4 cut(s) 35, 558, 566, 663
MboI GATC 4 cut(s) 33, 556, 564, 661
MboII GAAGA 4 cut(s) 70, 412, 757, 796
MlsI TGGCCA 1 cut(s) 657
MluCI AATT 2 cut(s) 53, 415
MluNI TGGCCA 1 cut(s) 657
MlyI GAGTC 2 cut(s) 70, 512
MnlI CCTC 4 cut(s) 87, 415, 550, 641
Mox20I TGGCCA 1 cut(s) 657
MroNI GCCGGC 1 cut(s) 667
MroXI GAANNNNTTC 1 cut(s) 366
MscI TGGCCA 1 cut(s) 657
MseI TTAA 5 cut(s) 90, 293, 383, 483, 549
Msp20I TGGCCA 1 cut(s) 657
MspA1I CMGCKG 2 cut(s) 149, 689
MspI CCGG 2 cut(s) 453, 668
MspR9I CCNGG 2 cut(s) 442, 659
MvaI CCWGG 2 cut(s) 442, 659
MwoI GCNNNNNNNGC 5 cut(s) 437, 551, 578, 677, 686
NaeI GCCGGC 1 cut(s) 669
NdeII GATC 4 cut(s) 33, 556, 564, 661
NgoMIV GCCGGC 1 cut(s) 667
NlaIII CATG 5 cut(s) 184, 203, 332, 440, 464
NlaIV GGNNCC 1 cut(s) 627
NmuCI GTSAC 1 cut(s) 637
NspI RCATGY 3 cut(s) 332, 440, 464
PagI TCATGA 1 cut(s) 199
PaqCI CACCTGC 1 cut(s) 563
PciI ACATGT 1 cut(s) 328
PdiI GCCGGC 1 cut(s) 669
PdmI GAANNNNTTC 1 cut(s) 366
PfeI GAWTC 3 cut(s) 71, 307, 374
PflMI CCANNNNNTGG 1 cut(s) 317
PkrI GCNGC 7 cut(s) 408, 583, 682, 685, 688, 712, 715
PleI GAGTC 2 cut(s) 69, 511
PpsI GAGTC 2 cut(s) 69, 511
PscI ACATGT 1 cut(s) 328
Psp6I CCWGG 2 cut(s) 440, 657
PspGI CCWGG 2 cut(s) 440, 657
PspN4I GGNNCC 1 cut(s) 627
PspPI GGNCC 2 cut(s) 143, 625
PstI CTGCAG 1 cut(s) 688
PstNI CAGNNNCTG 1 cut(s) 641
PvuII CAGCTG 1 cut(s) 149
RsaI GTAC 2 cut(s) 621, 736
RsaNI GTAC 2 cut(s) 620, 735
SaqAI TTAA 5 cut(s) 90, 293, 383, 483, 549
SatI GCNGC 7 cut(s) 407, 582, 681, 684, 687, 711, 714
Sau3AI GATC 4 cut(s) 33, 556, 564, 661
Sau96I GGNCC 2 cut(s) 143, 625
SchI GAGTC 2 cut(s) 70, 512
ScrFI CCNGG 2 cut(s) 442, 659
SetI ASST 9 cut(s) 33, 151, 229, 293, 496, 542, 577, 682, 757
SfaNI GCATC 3 cut(s) 393, 725, 784
SfcI CTRYAG 2 cut(s) 684, 723
SinI GGWCC 1 cut(s) 625
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 2 cut(s) 53, 415
SsiI CCGC 1 cut(s) 689
SspMI CTAG 1 cut(s) 240
StyD4I CCNGG 2 cut(s) 440, 657
TaaI ACNGT 2 cut(s) 637, 724
TaiI ACGT 2 cut(s) 33, 293
TaqI TCGA 1 cut(s) 792
TasI AATT 2 cut(s) 53, 415
TfiI GAWTC 3 cut(s) 71, 307, 374
Tru1I TTAA 5 cut(s) 90, 293, 383, 483, 549
Tru9I TTAA 5 cut(s) 90, 293, 383, 483, 549
TscAI CASTG 4 cut(s) 522, 597, 646, 747
TseFI GTSAC 1 cut(s) 637
TseI GCWGC 7 cut(s) 406, 581, 680, 683, 686, 710, 713
Tsp45I GTSAC 1 cut(s) 637
TspDTI ATGAA 2 cut(s) 197, 402
TspGWI ACGGA 1 cut(s) 607
TspRI CASTG 4 cut(s) 522, 597, 646, 747
Van91I CCANNNNNTGG 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 625
XagI CCTNNNNNAGG 1 cut(s) 81
XceI RCATGY 3 cut(s) 332, 440, 464
XmiI GTMKAC 1 cut(s) 506
XmnI GAANNNNTTC 1 cut(s) 366
XspI CTAG 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.