pycom14g04570

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009951
Physical Location & Seq
Forward (+)
4191653 .. 4192999
1347 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g04570.1

Sequence Viewer

Length: 1347 bp
ATGGCTCAAGAAATTGAAAAGTACCTAATGGAAAAGTTCCGGAATGGACTTGATGGGAGAGACAAAAGTATCTCTAAGATTCCAAAGTACAGCCAATTTCAAGAAATAAAAATTTTACTTGATGACATTTTCAGCTCATCCTTGCATGATTCATCCATCAGAAATAAGCTTTACGACCTCAACAATGTTTTGGCTGAGTGCCAAATGCTTTCGAGAAAACACGGTTTCAACTCTCCCAAGGAGCTACTCACCATAAACAGAATCAGAAGGGATTTAAACAGGATCAAGAGGGAGCTCAAGTTTATAAAGGATAATAAAAGATCATTGCATGGAAATACCGATCCACAACCTAATAATGGTCAAGCAAGCTCAACGGGTGGAGAGCTTTCGAGGTGGACTACGCGTTCACTAGATGCATCCAAGGTTTATGGATTTGATGATGATGTGATTTTGATGAAGAAGTTGCTTTTACAGCAAGGAACTGATGATCGGTTCAGGGCAGTAGGCATTGTTGGTAGGGAAGGTATAGGAAAAACAACACTTTGCCAGGTCTTATTCAACGAACAAGAAGTGAAAAACACATTCCTTCCGAGGATCTGGGTGTGCATGGCCAGACACCCGGATGACGACGACAATGAGGTTCCGAAAATAGTAATTGTCAAAAGAATGTTGATGCACCTCGGAGTTGGAAAGGAGATGGTCAAGTCCGTTTGTGAGAAGCATGGCCTCGGGGGACTGCTGTATGCTCTTCGCCTGCAATTGATGGGCAAGAGGTATCTGATCGTGCTTGATCATGCCAGGGAGACGGACTCATGGTGTGGACAGCTGGATTCGTGTTTGACTCGTGATAAGGAATGGGATGACGGCCTTGCATTTGGACTCCCAAAAGGGCATGGGGGAAGACTCATAGTCACAAGTCGGAATGAAGATATAGCTAAAATGATGGTCGGGAAGGAAAATATACATCACCTTCTGCCCCTTTCGGATCATGAAAGCTGCTGGGCGATATTCATAGACGCAGTTGGTCGGATTCCGTCCAGTCCCTCAAACTTGGAAGATGTGACAGATGATGAAGGCTTATGGGCGATATTAAAGGACGCTTTTGAGAATAATCGAACTCCATCCAGACCCTCAAACTTGGAAGATCTGATAAATAATGAAAGCTTCTGGGCGATATACCTGGACGGTGTTAAGAATAATTGGATTTCAATTCCATCCGGTCCCTCAGACTTGGAAGATCTGAAATTGGAAGTCAAAGAAAAATGTGGGGGCATTCCATTAGCATTAAAGATGATGGGACAGGCAACGCAAGAATCACTTAAGGTCATCCGTGGCACGAATCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

449

Amino Acids

50.76

Weight (kDa)

6.84

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 157 - 342 3.9e-16 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009951)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19060 AT5G45440 AT5G45490 AT5G45490
fragaria_vesca FvH4_6g22150
malus_domestica MD12G1059800.v1.1 MD14G1055400.v1.1
prunus_persica Prupe.7G078500_v2.0.a1 Prupe.7G078700_v2.0.a1
pyrus_communis pycom14g04570
rosa_chinensis RchiOBHm_Chr3g0479501
rosa_laevigata RLG00000023564
rosa_multiflora Rmu_sc0004732.1_g000011
rosa_roxburghii Rroxscaffold_6G00402200
rosa_rugosa Rorug03G0175800 Rorug03G0175900
rosa_samantha Rh3AG226800 Rh3BG259900 Rh3CG256600 Rh3DG252400
rosa_wichuraiana Rw3G020260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 305
AccII CGCG 1 cut(s) 403
AccIII TCCGGA 1 cut(s) 39
AclWI GGATC 4 cut(s) 290, 335, 602, 993
AcoI YGGCCR 1 cut(s) 609
AcsI RAATTY 1 cut(s) 111
AfaI GTAC 2 cut(s) 23, 89
AfiI CCNNNNNNNGG 1 cut(s) 356
AflII CTTAAG 1 cut(s) 1319
AflIII ACRYGT 1 cut(s) 401
AgsI TTSAA 5 cut(s) 17, 101, 229, 559, 1209
AhdI GACNNNNNGTC 1 cut(s) 908
AjnI CCWGG 3 cut(s) 546, 797, 1179
AloI GAACNNNNNNTCC 2 cut(s) 388, 420
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 2 cut(s) 54, 797
AlwI GGATC 4 cut(s) 290, 335, 602, 993
Ama87I CYCGRG 1 cut(s) 728
Aor13HI TCCGGA 1 cut(s) 39
AoxI GGCC 3 cut(s) 609, 724, 865
ApeKI GCWGC 1 cut(s) 996
ApoI RAATTY 1 cut(s) 111
AspS9I GGNCC 1 cut(s) 1220
AsuC2I CCSGG 1 cut(s) 620
AsuHPI GGTGA 2 cut(s) 241, 959
AvaI CYCGRG 1 cut(s) 728
AvaII GGWCC 1 cut(s) 1220
BaeI ACNNNNGTAYC 2 cut(s) 52, 85
BalI TGGCCA 1 cut(s) 611
BanII GRGCYC 1 cut(s) 297
BauI CACGAG 1 cut(s) 843
BbsI GAAGAC 1 cut(s) 907
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 1 cut(s) 983
BccI CCATC 8 cut(s) 47, 164, 691, 757, 937, 1129, 1222, 1288
BceAI ACGGC 1 cut(s) 880
BciT130I CCWGG 3 cut(s) 548, 799, 1181
BclI TGATCA 1 cut(s) 790
BcnI CCSGG 1 cut(s) 620
BcoDI GTCTC 2 cut(s) 54, 797
BfaI CTAG 1 cut(s) 410
BfrI CTTAAG 1 cut(s) 1319
BglII AGATCT 2 cut(s) 1144, 1237
BisI GCNGC 1 cut(s) 997
BlsI GCNGC 1 cut(s) 998
Bme1390I CCNGG 4 cut(s) 548, 620, 799, 1181
Bme18I GGWCC 1 cut(s) 1220
BmeRI GACNNNNNGTC 1 cut(s) 908
BmeT110I CYCGRG 1 cut(s) 728
BmgT120I GGNCC 1 cut(s) 1220
BmiI GGNNCC 2 cut(s) 642, 1222
BmrFI CCNGG 4 cut(s) 548, 620, 799, 1181
BmsI GCATC 3 cut(s) 403, 425, 663
BpiI GAAGAC 1 cut(s) 907
BplI GAGNNNNNCTC 2 cut(s) 794, 826
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 620
BsaJI CCNNGG 7 cut(s) 237, 420, 590, 679, 727, 798, 1330
BsaWI WCCGGW 2 cut(s) 39, 1217
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse1I ACTGG 1 cut(s) 1038
Bse3DI GCAATG 1 cut(s) 323
BseAI TCCGGA 1 cut(s) 39
BseBI CCWGG 3 cut(s) 548, 799, 1181
BseDI CCNNGG 7 cut(s) 237, 420, 590, 679, 727, 798, 1330
BseGI GGATG 8 cut(s) 137, 152, 416, 628, 865, 1121, 1214, 1326
BseLI CCNNNNNNNGG 1 cut(s) 356
BseMI GCAATG 1 cut(s) 323
BseMII CTCAG 2 cut(s) 186, 1239
BseNI ACTGG 1 cut(s) 1038
BseXI GCAGC 1 cut(s) 983
BseYI CCCAGC 1 cut(s) 999
Bsh1236I CGCG 1 cut(s) 403
BshFI GGCC 3 cut(s) 611, 726, 867
BsiHKAI GWGCWC 1 cut(s) 297
BsiHKCI CYCGRG 1 cut(s) 728
BsiSI CCGG 3 cut(s) 40, 620, 1218
BslFI GGGAC 4 cut(s) 747, 1026, 1206, 1311
BslI CCNNNNNNNGG 1 cut(s) 356
BsmAI GTCTC 2 cut(s) 54, 797
BsmBI CGTCTC 1 cut(s) 797
BsmFI GGGAC 4 cut(s) 747, 1026, 1206, 1311
BsmI GAATGC 1 cut(s) 1272
BsnI GGCC 3 cut(s) 611, 726, 867
BsoBI CYCGRG 1 cut(s) 728
Bsp1286I GDGCHC 1 cut(s) 297
Bsp13I TCCGGA 1 cut(s) 39
BspANI GGCC 3 cut(s) 611, 726, 867
BspCNI CTCAG 2 cut(s) 187, 1238
BspEI TCCGGA 1 cut(s) 39
BspFNI CGCG 1 cut(s) 403
BspHI TCATGA 1 cut(s) 988
BspLI GGNNCC 2 cut(s) 642, 1222
BspPI GGATC 4 cut(s) 290, 335, 602, 993
BspQI GCTCTTC 1 cut(s) 753
BspTI CTTAAG 1 cut(s) 1319
BsrDI GCAATG 1 cut(s) 323
BsrI ACTGG 1 cut(s) 1038
BssECI CCNNGG 7 cut(s) 237, 420, 590, 679, 727, 798, 1330
BssSI CACGAG 1 cut(s) 843
BssT1I CCWWGG 2 cut(s) 237, 420
Bst2BI CACGAG 1 cut(s) 843
Bst2UI CCWGG 3 cut(s) 548, 799, 1181
Bst4CI ACNGT 2 cut(s) 224, 1187
Bst6I CTCTTC 1 cut(s) 753
BstAFI CTTAAG 1 cut(s) 1319
BstC8I GCNNGC 2 cut(s) 367, 755
BstDEI CTNAG 4 cut(s) 75, 195, 1225, 1344
BstDSI CCRYGG 1 cut(s) 1330
BstF5I GGATG 8 cut(s) 137, 152, 416, 628, 865, 1121, 1214, 1326
BstFNI CGCG 1 cut(s) 403
BstMAI GTCTC 2 cut(s) 54, 797
BstMWI GCNNNNNNNGC 1 cut(s) 472
BstNI CCWGG 3 cut(s) 548, 799, 1181
BstSCI CCNGG 4 cut(s) 546, 618, 797, 1179
BstUI CGCG 1 cut(s) 403
BstV1I GCAGC 1 cut(s) 983
BstV2I GAAGAC 1 cut(s) 907
BstX2I RGATCY 3 cut(s) 594, 1144, 1237
BstYI RGATCY 3 cut(s) 594, 1144, 1237
BsuRI GGCC 3 cut(s) 611, 726, 867
BtgI CCRYGG 1 cut(s) 1330
BtsCI GGATG 8 cut(s) 137, 152, 416, 628, 865, 1121, 1214, 1326
Cac8I GCNNGC 2 cut(s) 367, 755
CciI TCATGA 1 cut(s) 988
Cfr13I GGNCC 1 cut(s) 1220
CseI GACGC 2 cut(s) 1025, 1106
Csp6I GTAC 2 cut(s) 22, 88
CviAII CATG 8 cut(s) 146, 329, 607, 722, 794, 813, 893, 989
CviQI GTAC 2 cut(s) 22, 88
DdeI CTNAG 4 cut(s) 75, 195, 1225, 1344
DraI TTTAAA 1 cut(s) 276
DriI GACNNNNNGTC 1 cut(s) 908
EaeI YGGCCR 1 cut(s) 609
Eam1104I CTCTTC 1 cut(s) 753
Eam1105I GACNNNNNGTC 1 cut(s) 908
EarI CTCTTC 1 cut(s) 753
Ecl136II GAGCTC 1 cut(s) 295
Eco130I CCWWGG 2 cut(s) 237, 420
Eco24I GRGCYC 1 cut(s) 297
Eco47I GGWCC 1 cut(s) 1220
Eco53kI GAGCTC 1 cut(s) 295
Eco88I CYCGRG 1 cut(s) 728
EcoICRI GAGCTC 1 cut(s) 295
EcoRII CCWGG 3 cut(s) 546, 797, 1179
EcoT14I CCWWGG 2 cut(s) 237, 420
EcoT22I ATGCAT 1 cut(s) 418
EcoT38I GRGCYC 1 cut(s) 297
ErhI CCWWGG 2 cut(s) 237, 420
Esp3I CGTCTC 1 cut(s) 797
FaeI CATG 8 cut(s) 149, 332, 610, 725, 797, 816, 896, 992
FalI AAGNNNNNCTT 2 cut(s) 1302, 1334
FaqI GGGAC 4 cut(s) 747, 1026, 1206, 1311
FatI CATG 8 cut(s) 145, 328, 606, 721, 793, 812, 892, 988
FbaI TGATCA 1 cut(s) 790
Fnu4HI GCNGC 1 cut(s) 997
FokI GGATG 8 cut(s) 124, 139, 403, 635, 872, 1108, 1201, 1313
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 1 cut(s) 997
FspBI CTAG 1 cut(s) 410
GluI GCNGC 1 cut(s) 997
GsaI CCCAGC 1 cut(s) 1003
HaeIII GGCC 3 cut(s) 611, 726, 867
HapII CCGG 3 cut(s) 40, 620, 1218
HgaI GACGC 2 cut(s) 1025, 1106
Hin1II CATG 8 cut(s) 149, 332, 610, 725, 797, 816, 896, 992
HindIII AAGCTT 2 cut(s) 167, 1162
HpaII CCGG 3 cut(s) 40, 620, 1218
HphI GGTGA 2 cut(s) 241, 959
Hpy166II GTNNAC 3 cut(s) 396, 407, 821
Hpy188III TCNNGA 9 cut(s) 8, 40, 101, 213, 286, 845, 949, 989, 1125
Hpy8I GTNNAC 3 cut(s) 396, 407, 821
Hpy99I CGWCG 1 cut(s) 632
HpyAV CCTTC 6 cut(s) 261, 515, 596, 946, 980, 1067
HpyCH4III ACNGT 2 cut(s) 224, 1187
HpyCH4V TGCA 7 cut(s) 145, 328, 416, 606, 676, 757, 872
HpyF10VI GCNNNNNNNGC 1 cut(s) 472
HpyF3I CTNAG 4 cut(s) 75, 195, 1225, 1344
Hsp92II CATG 8 cut(s) 149, 332, 610, 725, 797, 816, 896, 992
Kpn2I TCCGGA 1 cut(s) 39
Ksp22I TGATCA 1 cut(s) 790
LguI GCTCTTC 1 cut(s) 753
LmnI GCTCC 2 cut(s) 241, 292
Lsp1109I GCAGC 1 cut(s) 983
LweI GCATC 3 cut(s) 403, 425, 663
MaeI CTAG 1 cut(s) 410
MaeIII GTNAC 2 cut(s) 910, 1060
MboII GAAGA 7 cut(s) 469, 740, 912, 938, 1067, 1154, 1247
MfeI CAATTG 1 cut(s) 758
MflI RGATCY 3 cut(s) 594, 1144, 1237
MhlI GDGCHC 1 cut(s) 297
MlsI TGGCCA 1 cut(s) 611
MluCI AATT 8 cut(s) 12, 95, 111, 654, 758, 1198, 1209, 1244
MluI ACGCGT 1 cut(s) 401
MluNI TGGCCA 1 cut(s) 611
MlyI GAGTC 4 cut(s) 803, 835, 873, 897
MmeI TCCRAC 3 cut(s) 667, 899, 1007
Mox20I TGGCCA 1 cut(s) 611
Mph1103I ATGCAT 1 cut(s) 418
MroI TCCGGA 1 cut(s) 39
MscI TGGCCA 1 cut(s) 611
MseI TTAA 5 cut(s) 275, 1091, 1191, 1286, 1320
MslI CAYNNNNRTG 1 cut(s) 621
Msp20I TGGCCA 1 cut(s) 611
MspA1I CMGCKG 1 cut(s) 826
MspCI CTTAAG 1 cut(s) 1319
MspI CCGG 3 cut(s) 40, 620, 1218
MspR9I CCNGG 4 cut(s) 548, 620, 799, 1181
MunI CAATTG 1 cut(s) 758
Mva1269I GAATGC 1 cut(s) 1272
MvaI CCWGG 3 cut(s) 548, 799, 1181
MvnI CGCG 1 cut(s) 403
MwoI GCNNNNNNNGC 1 cut(s) 472
NciI CCSGG 1 cut(s) 620
NlaIII CATG 8 cut(s) 149, 332, 610, 725, 797, 816, 896, 992
NlaIV GGNNCC 2 cut(s) 642, 1222
NmuCI GTSAC 2 cut(s) 910, 1060
NsiI ATGCAT 1 cut(s) 418
PagI TCATGA 1 cut(s) 988
PciSI GCTCTTC 1 cut(s) 753
PctI GAATGC 1 cut(s) 1272
PfeI GAWTC 7 cut(s) 79, 149, 261, 830, 1030, 1313, 1339
PkrI GCNGC 1 cut(s) 998
PleI GAGTC 4 cut(s) 803, 835, 873, 897
PpsI GAGTC 4 cut(s) 803, 835, 873, 897
PsiI TTATAA 1 cut(s) 305
Psp124BI GAGCTC 1 cut(s) 297
Psp6I CCWGG 3 cut(s) 546, 797, 1179
PspFI CCCAGC 1 cut(s) 999
PspGI CCWGG 3 cut(s) 546, 797, 1179
PspN4I GGNNCC 2 cut(s) 642, 1222
PspPI GGNCC 1 cut(s) 1220
PsuI RGATCY 3 cut(s) 594, 1144, 1237
PvuII CAGCTG 1 cut(s) 826
RsaI GTAC 2 cut(s) 23, 89
RsaNI GTAC 2 cut(s) 22, 88
RseI CAYNNNNRTG 1 cut(s) 621
SacI GAGCTC 1 cut(s) 297
SapI GCTCTTC 1 cut(s) 753
SaqAI TTAA 5 cut(s) 275, 1091, 1191, 1286, 1320
SatI GCNGC 1 cut(s) 997
Sau96I GGNCC 1 cut(s) 1220
SchI GAGTC 4 cut(s) 803, 835, 873, 897
ScrFI CCNGG 4 cut(s) 548, 620, 799, 1181
SduI GDGCHC 1 cut(s) 297
SfaNI GCATC 3 cut(s) 403, 425, 663
SinI GGWCC 1 cut(s) 1220
SmiMI CAYNNNNRTG 1 cut(s) 621
SmlI CTYRAG 3 cut(s) 6, 296, 1319
SmoI CTYRAG 3 cut(s) 6, 296, 1319
Sse9I AATT 8 cut(s) 12, 95, 111, 654, 758, 1198, 1209, 1244
SspMI CTAG 1 cut(s) 410
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 4 cut(s) 546, 618, 797, 1179
StyI CCWWGG 2 cut(s) 237, 420
TaaI ACNGT 2 cut(s) 224, 1187
TaqI TCGA 3 cut(s) 212, 389, 1114
TasI AATT 8 cut(s) 12, 95, 111, 654, 758, 1198, 1209, 1244
TatI WGTACW 1 cut(s) 87
TfiI GAWTC 7 cut(s) 79, 149, 261, 830, 1030, 1313, 1339
Tru1I TTAA 5 cut(s) 275, 1091, 1191, 1286, 1320
Tru9I TTAA 5 cut(s) 275, 1091, 1191, 1286, 1320
TseFI GTSAC 2 cut(s) 910, 1060
TseI GCWGC 1 cut(s) 996
Tsp45I GTSAC 2 cut(s) 910, 1060
TspDTI ATGAA 7 cut(s) 141, 470, 939, 1000, 1005, 1086, 1173
TspGWI ACGGA 4 cut(s) 697, 821, 1023, 1319
Vha464I CTTAAG 1 cut(s) 1319
VpaK11BI GGWCC 1 cut(s) 1220
XapI RAATTY 1 cut(s) 111
XspI CTAG 1 cut(s) 410
Zsp2I ATGCAT 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.