Rw3G020260

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0009951
Physical Location & Seq
Forward (+)
23714192 .. 23715952
1761 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G020260.1

Sequence Viewer

Length: 1185 bp
ATGGCTCAAGAAATGGAACTACACCTGAAAGACAAGTTCATGGACGGCCTTGATCATATGAAGGACAAAAGCATCTCCAAGATTCCAAGGTACAACCAATTTCAAGAGATAAAGAGATTGCTAGAAGAAATGTTCAGCTCATCCACGCCTGCAATAGTCAAGTTTCCGAGTGCAACCAATTTCAGGGACAAGCTTTACAAGCTCAACAATCTTTTGACCGACTGCAAGATGCTATCAAGAAAACATGGTTTCAACTCTCCGGAGGAGCTAATTACTATTAATAGAATCCGGAGAGACTTGAGACAGATCAAGAGGGATCTCCAGGCCATTAAGGATGCGTCAAATCGAGACATTAATGAACAACGTATTAATGGTGATTCAAGCTCACATGATAGAGACATTTTGAGATGGACTACTCGTGCAGTAGATGCAACAAAGGTTTATGGACTTGATGATGAGGTGTTGTCCATGGAAAACTTGCTTCTCAAGAACGGAAGTGATGTTCATCAGTTCAAGGCAATAGGCATTGTCGGCAGGGATGCTATAGGAAAGACAACGCTTTGCCAACTGATGTTCAACAAAGATGAAGTGAAAAACAGCTTCAATCCAAGGATCTGGGTGTGCATGGCCAGACACCCGGATGACGACGAGGATGAGGATCTTAAATTAGCAATTGTCAAAAGAATGTTGAGGTACCTTGGAGTCGAAGAGGCAATAGTCAAGTCGATTTGTGATGTAAAACCTGGCCTAGAAGGACTGCTATGTGCTCTTTATCTGCAATTGTTGGGCAAGAGGTATCTAATTGTGCTTGATGATGCTAGGGAGACAGACTCATGGTATAAAGAGTTGGCTTCCAGTTTGACTCATGATAAGAAATGGGGTGACAGTTTCGCATTTGGATTTCCAAAAGGCAGTGGAGGAAGAGTCATAGTTACAAGCAGAAATGAAGACATAGCAAATATGATGGTTGGGAAGGAAAATATACATCACCTTGAGCCCATGTTGGACACTGAAAGCTGCTGGAAAATATTCAAAGACTCGGTTGACGAATATCTGTTACCTACCATTCCCTCAATCTTGGAAGAACTAAAATCAGAAGTAAAACAAAAATGTGGTGGCCTTCCGTTAGCTGCAAAAATGATGGGGCGTGCAATGCTGGAGGAAGAAGAGCGTACTAGAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

394

Amino Acids

45.25

Weight (kDa)

6.35

Isoelectric Point (pI)

37.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 169 - 348 3.7e-20 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009951)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19060 AT5G45440 AT5G45490 AT5G45490
fragaria_vesca FvH4_6g22150
malus_domestica MD12G1059800.v1.1 MD14G1055400.v1.1
prunus_persica Prupe.7G078500_v2.0.a1 Prupe.7G078700_v2.0.a1
pyrus_communis pycom14g04570
rosa_chinensis RchiOBHm_Chr3g0479501
rosa_laevigata RLG00000023564
rosa_multiflora Rmu_sc0004732.1_g000011
rosa_roxburghii Rroxscaffold_6G00402200
rosa_rugosa Rorug03G0175800 Rorug03G0175900
rosa_samantha Rh3AG226800 Rh3BG259900 Rh3CG256600 Rh3DG252400
rosa_wichuraiana Rw3G020260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 693
AccB1I GGYRCC 1 cut(s) 693
AccIII TCCGGA 2 cut(s) 259, 288
AclWI GGATC 3 cut(s) 324, 620, 666
AcoI YGGCCR 1 cut(s) 627
AfaI GTAC 3 cut(s) 92, 695, 1174
AfiI CCNNNNNNNGG 1 cut(s) 183
AgsI TTSAA 7 cut(s) 104, 253, 381, 514, 577, 604, 1033
AjnI CCWGG 2 cut(s) 321, 742
AloI GAACNNNNNNTCC 2 cut(s) 486, 518
AluBI AGCT 9 cut(s) 138, 193, 202, 268, 384, 600, 1017, 1130, 1182
AluI AGCT 9 cut(s) 138, 193, 202, 268, 384, 600, 1017, 1130, 1182
Alw21I GWGCWC 1 cut(s) 769
Alw26I GTCTC 5 cut(s) 288, 295, 342, 390, 818
AlwI GGATC 3 cut(s) 324, 620, 666
Aor13HI TCCGGA 2 cut(s) 259, 288
AoxI GGCC 5 cut(s) 46, 324, 627, 745, 1117
ApeKI GCWGC 2 cut(s) 1017, 1130
AseI ATTAAT 3 cut(s) 279, 354, 369
Asp700I GAANNNNTTC 1 cut(s) 1028
Asp718I GGTACC 1 cut(s) 693
AsuC2I CCSGG 1 cut(s) 638
AsuHPI GGTGA 3 cut(s) 386, 893, 980
BalI TGGCCA 1 cut(s) 629
BanI GGYRCC 1 cut(s) 693
BanII GRGCYC 1 cut(s) 999
BauI CACGAG 1 cut(s) 417
BbsI GAAGAC 1 cut(s) 954
Bbv12I GWGCWC 1 cut(s) 769
BbvI GCAGC 2 cut(s) 1004, 1117
BccI CCATC 3 cut(s) 402, 958, 1135
BceAI ACGGC 1 cut(s) 61
BciT130I CCWGG 2 cut(s) 323, 744
BclI TGATCA 1 cut(s) 52
BcnI CCSGG 1 cut(s) 638
BcoDI GTCTC 5 cut(s) 288, 295, 342, 390, 818
BfaI CTAG 5 cut(s) 122, 749, 819, 1176, 1183
BfmI CTRYAG 1 cut(s) 543
BisI GCNGC 2 cut(s) 1018, 1131
BlsI GCNGC 2 cut(s) 1019, 1132
Bme1390I CCNGG 3 cut(s) 323, 638, 744
BmiI GGNNCC 1 cut(s) 695
BmrFI CCNGG 3 cut(s) 323, 638, 744
BmsI GCATC 6 cut(s) 81, 219, 325, 418, 529, 805
BpiI GAAGAC 1 cut(s) 954
BplI GAGNNNNNCTC 2 cut(s) 815, 847
BpmI CTGGAG 2 cut(s) 305, 1178
BpuEI CTTGAG 3 cut(s) 319, 470, 1013
BpuMI CCSGG 1 cut(s) 638
BsaBI GATNNNNATC 2 cut(s) 504, 657
BsaJI CCNNGG 4 cut(s) 86, 468, 608, 697
BsaWI WCCGGW 2 cut(s) 259, 288
BsaXI ACNNNNNCTCC 2 cut(s) 909, 939
Bsc4I CCNNNNNNNGG 1 cut(s) 183
Bse1I ACTGG 1 cut(s) 855
Bse3DI GCAATG 1 cut(s) 1158
Bse8I GATNNNNATC 2 cut(s) 504, 657
BseAI TCCGGA 2 cut(s) 259, 288
BseBI CCWGG 2 cut(s) 323, 744
BseDI CCNNGG 4 cut(s) 86, 468, 608, 697
BseGI GGATG 5 cut(s) 140, 340, 544, 646, 658
BseJI GATNNNNATC 2 cut(s) 504, 657
BseLI CCNNNNNNNGG 1 cut(s) 183
BseMI GCAATG 1 cut(s) 1158
BseNI ACTGG 1 cut(s) 855
BseRI GAGGAG 1 cut(s) 278
BseXI GCAGC 2 cut(s) 1004, 1117
BsgI GTGCAG 1 cut(s) 441
BshFI GGCC 5 cut(s) 48, 326, 629, 747, 1119
BshNI GGYRCC 1 cut(s) 693
BsiHKAI GWGCWC 1 cut(s) 769
BsiSI CCGG 3 cut(s) 260, 289, 638
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 1 cut(s) 183
BsmAI GTCTC 5 cut(s) 288, 295, 342, 390, 818
BsmFI GGGAC 1 cut(s) 200
BsnI GGCC 5 cut(s) 48, 326, 629, 747, 1119
Bsp1286I GDGCHC 2 cut(s) 769, 999
Bsp13I TCCGGA 2 cut(s) 259, 288
Bsp143I GATC 5 cut(s) 52, 306, 316, 612, 658
Bsp19I CCATGG 1 cut(s) 468
BspANI GGCC 5 cut(s) 48, 326, 629, 747, 1119
BspEI TCCGGA 2 cut(s) 259, 288
BspHI TCATGA 1 cut(s) 865
BspLI GGNNCC 1 cut(s) 695
BspPI GGATC 3 cut(s) 324, 620, 666
BspQI GCTCTTC 1 cut(s) 1161
BspT107I GGYRCC 1 cut(s) 693
BsrDI GCAATG 1 cut(s) 1158
BsrI ACTGG 1 cut(s) 855
BssECI CCNNGG 4 cut(s) 86, 468, 608, 697
BssMI GATC 5 cut(s) 52, 306, 316, 612, 658
BssSI CACGAG 1 cut(s) 417
BssT1I CCWWGG 4 cut(s) 86, 468, 608, 697
Bst2BI CACGAG 1 cut(s) 417
Bst2UI CCWGG 2 cut(s) 323, 744
Bst4CI ACNGT 1 cut(s) 887
Bst6I CTCTTC 3 cut(s) 702, 916, 1161
BstAPI GCANNNNNTGC 1 cut(s) 428
BstC8I GCNNGC 2 cut(s) 150, 1149
BstDSI CCRYGG 1 cut(s) 468
BstF5I GGATG 5 cut(s) 140, 340, 544, 646, 658
BstKTI GATC 5 cut(s) 55, 309, 319, 615, 661
BstMAI GTCTC 5 cut(s) 288, 295, 342, 390, 818
BstMBI GATC 5 cut(s) 52, 306, 316, 612, 658
BstMWI GCNNNNNNNGC 4 cut(s) 199, 428, 531, 1153
BstNI CCWGG 2 cut(s) 323, 744
BstSCI CCNGG 3 cut(s) 321, 636, 742
BstSFI CTRYAG 1 cut(s) 543
BstV1I GCAGC 2 cut(s) 1004, 1117
BstV2I GAAGAC 1 cut(s) 954
BstX2I RGATCY 3 cut(s) 316, 612, 658
BstXI CCANNNNNNTGG 1 cut(s) 615
BstYI RGATCY 3 cut(s) 316, 612, 658
BsuRI GGCC 5 cut(s) 48, 326, 629, 747, 1119
BtgI CCRYGG 1 cut(s) 468
BtsCI GGATG 5 cut(s) 140, 340, 544, 646, 658
BtsI GCAGTG 1 cut(s) 919
BtsIMutI CAGTG 2 cut(s) 919, 1008
Cac8I GCNNGC 2 cut(s) 150, 1149
CciI TCATGA 1 cut(s) 865
CseI GACGC 1 cut(s) 327
Csp6I GTAC 3 cut(s) 91, 694, 1173
CviAII CATG 8 cut(s) 40, 245, 389, 469, 625, 834, 866, 1000
CviQI GTAC 3 cut(s) 91, 694, 1173
DpnI GATC 5 cut(s) 54, 308, 318, 614, 660
DpnII GATC 5 cut(s) 52, 306, 316, 612, 658
EaeI YGGCCR 1 cut(s) 627
Eam1104I CTCTTC 3 cut(s) 702, 916, 1161
EarI CTCTTC 3 cut(s) 702, 916, 1161
Eco130I CCWWGG 4 cut(s) 86, 468, 608, 697
Eco24I GRGCYC 1 cut(s) 999
EcoRII CCWGG 2 cut(s) 321, 742
EcoT14I CCWWGG 4 cut(s) 86, 468, 608, 697
EcoT38I GRGCYC 1 cut(s) 999
ErhI CCWWGG 4 cut(s) 86, 468, 608, 697
FaeI CATG 8 cut(s) 43, 248, 392, 472, 628, 837, 869, 1003
FaqI GGGAC 1 cut(s) 200
FatI CATG 8 cut(s) 39, 244, 388, 468, 624, 833, 865, 999
FauNDI CATATG 1 cut(s) 57
FbaI TGATCA 1 cut(s) 52
Fnu4HI GCNGC 2 cut(s) 1018, 1131
FokI GGATG 5 cut(s) 127, 347, 551, 653, 665
FriOI GRGCYC 1 cut(s) 999
Fsp4HI GCNGC 2 cut(s) 1018, 1131
FspBI CTAG 5 cut(s) 122, 749, 819, 1176, 1183
GluI GCNGC 2 cut(s) 1018, 1131
GsuI CTGGAG 2 cut(s) 305, 1178
HaeIII GGCC 5 cut(s) 48, 326, 629, 747, 1119
HapII CCGG 3 cut(s) 260, 289, 638
HgaI GACGC 1 cut(s) 327
Hin1II CATG 8 cut(s) 43, 248, 392, 472, 628, 837, 869, 1003
HincII GTYRAC 1 cut(s) 1045
HindII GTYRAC 1 cut(s) 1045
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 8 cut(s) 82, 285, 377, 702, 830, 862, 924, 1037
HpaII CCGG 3 cut(s) 260, 289, 638
HphI GGTGA 3 cut(s) 386, 893, 980
Hpy166II GTNNAC 1 cut(s) 1045
Hpy188I TCNGA 2 cut(s) 168, 1096
Hpy188III TCNNGA 9 cut(s) 8, 104, 237, 260, 289, 310, 347, 487, 866
Hpy8I GTNNAC 1 cut(s) 1045
Hpy99I CGWCG 1 cut(s) 650
HpyAV CCTTC 4 cut(s) 55, 746, 967, 1130
HpyCH4III ACNGT 1 cut(s) 887
HpyCH4IV ACGT 1 cut(s) 364
HpyCH4V TGCA 9 cut(s) 152, 173, 225, 422, 431, 624, 778, 1133, 1151
HpyF10VI GCNNNNNNNGC 4 cut(s) 199, 428, 531, 1153
HpySE526I ACGT 1 cut(s) 364
Hsp92II CATG 8 cut(s) 43, 248, 392, 472, 628, 837, 869, 1003
Kpn2I TCCGGA 2 cut(s) 259, 288
KpnI GGTACC 1 cut(s) 697
Ksp22I TGATCA 1 cut(s) 52
Kzo9I GATC 5 cut(s) 52, 306, 316, 612, 658
LguI GCTCTTC 1 cut(s) 1161
LmnI GCTCC 1 cut(s) 265
Lsp1109I GCAGC 2 cut(s) 1004, 1117
LweI GCATC 6 cut(s) 81, 219, 325, 418, 529, 805
MaeI CTAG 5 cut(s) 122, 749, 819, 1176, 1183
MaeII ACGT 1 cut(s) 364
MaeIII GTNAC 3 cut(s) 881, 931, 1056
MalI GATC 5 cut(s) 54, 308, 318, 614, 660
MboI GATC 5 cut(s) 52, 306, 316, 612, 658
MboII GAAGA 7 cut(s) 137, 719, 933, 959, 1094, 1175, 1178
MfeI CAATTG 2 cut(s) 672, 779
MflI RGATCY 3 cut(s) 316, 612, 658
MhlI GDGCHC 2 cut(s) 769, 999
MlsI TGGCCA 1 cut(s) 629
MluCI AATT 7 cut(s) 98, 178, 270, 665, 672, 779, 801
MluNI TGGCCA 1 cut(s) 629
MlyI GAGTC 5 cut(s) 711, 824, 856, 933, 1031
MmeI TCCRAC 1 cut(s) 984
Mox20I TGGCCA 1 cut(s) 629
MroI TCCGGA 2 cut(s) 259, 288
MroXI GAANNNNTTC 1 cut(s) 1028
MscI TGGCCA 1 cut(s) 629
MseI TTAA 5 cut(s) 279, 330, 354, 369, 663
MslI CAYNNNNRTG 1 cut(s) 639
Msp20I TGGCCA 1 cut(s) 629
MspI CCGG 3 cut(s) 260, 289, 638
MspR9I CCNGG 3 cut(s) 323, 638, 744
MunI CAATTG 2 cut(s) 672, 779
MvaI CCWGG 2 cut(s) 323, 744
MwoI GCNNNNNNNGC 4 cut(s) 199, 428, 531, 1153
NciI CCSGG 1 cut(s) 638
NcoI CCATGG 1 cut(s) 468
NdeI CATATG 1 cut(s) 57
NdeII GATC 5 cut(s) 52, 306, 316, 612, 658
NlaIII CATG 8 cut(s) 43, 248, 392, 472, 628, 837, 869, 1003
NlaIV GGNNCC 1 cut(s) 695
NmuCI GTSAC 1 cut(s) 881
PagI TCATGA 1 cut(s) 865
PciSI GCTCTTC 1 cut(s) 1161
PdmI GAANNNNTTC 1 cut(s) 1028
PfeI GAWTC 3 cut(s) 82, 285, 377
PkrI GCNGC 2 cut(s) 1019, 1132
PleI GAGTC 5 cut(s) 710, 824, 856, 932, 1031
PpsI GAGTC 5 cut(s) 710, 824, 856, 932, 1031
PshBI ATTAAT 3 cut(s) 279, 354, 369
Psp6I CCWGG 2 cut(s) 321, 742
PspGI CCWGG 2 cut(s) 321, 742
PspN4I GGNNCC 1 cut(s) 695
PsuI RGATCY 3 cut(s) 316, 612, 658
RsaI GTAC 3 cut(s) 92, 695, 1174
RsaNI GTAC 3 cut(s) 91, 694, 1173
RseI CAYNNNNRTG 1 cut(s) 639
SapI GCTCTTC 1 cut(s) 1161
SaqAI TTAA 5 cut(s) 279, 330, 354, 369, 663
SatI GCNGC 2 cut(s) 1018, 1131
Sau3AI GATC 5 cut(s) 52, 306, 316, 612, 658
SchI GAGTC 5 cut(s) 711, 824, 856, 933, 1031
ScrFI CCNGG 3 cut(s) 323, 638, 744
SduI GDGCHC 2 cut(s) 769, 999
SfaNI GCATC 6 cut(s) 81, 219, 325, 418, 529, 805
SfcI CTRYAG 1 cut(s) 543
SmiMI CAYNNNNRTG 1 cut(s) 639
SmlI CTYRAG 4 cut(s) 6, 298, 485, 992
SmoI CTYRAG 4 cut(s) 6, 298, 485, 992
Sse9I AATT 7 cut(s) 98, 178, 270, 665, 672, 779, 801
SspI AATATT 1 cut(s) 1029
SspMI CTAG 5 cut(s) 122, 749, 819, 1176, 1183
StyD4I CCNGG 3 cut(s) 321, 636, 742
StyI CCWWGG 4 cut(s) 86, 468, 608, 697
TaaI ACNGT 1 cut(s) 887
TaiI ACGT 1 cut(s) 367
TaqI TCGA 3 cut(s) 346, 705, 725
TaqII GACCGA 1 cut(s) 233
TasI AATT 7 cut(s) 98, 178, 270, 665, 672, 779, 801
TfiI GAWTC 3 cut(s) 82, 285, 377
Tru1I TTAA 5 cut(s) 279, 330, 354, 369, 663
Tru9I TTAA 5 cut(s) 279, 330, 354, 369, 663
TscAI CASTG 2 cut(s) 919, 1015
TseFI GTSAC 1 cut(s) 881
TseI GCWGC 2 cut(s) 1017, 1130
Tsp45I GTSAC 1 cut(s) 881
TspDTI ATGAA 6 cut(s) 28, 74, 372, 494, 600, 960
TspGWI ACGGA 2 cut(s) 507, 1113
TspRI CASTG 2 cut(s) 919, 1015
VspI ATTAAT 3 cut(s) 279, 354, 369
XmnI GAANNNNTTC 1 cut(s) 1028
XspI CTAG 5 cut(s) 122, 749, 819, 1176, 1183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.