Rh3CG256600

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0009951
Physical Location & Seq
Forward (+)
25030099 .. 25031946
1848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG256600.1

Sequence Viewer

Length: 1164 bp
ATGGCTCAAGAAATGGAACTGCACCTGAAAGACAAGTTCATGGACGGCCTTGATATGAAGGACAACAGCATCTCCAAGATTCCAAGGTACAACCAATTTCAAGAGATAAAGAGATTGCTAGAAGACATGTTCAGCTCATCCACGCCTGCAACAGTCAAGTTTCCGAGTGCAACCAATTTCAGGGACAAGCTTTACAGGCTCAACAATCTTTTGACCGACTGCAAGATGCTCTCAAGAAAACATGGTTTCAACTCTCCGGAGGAGCTAATTACTATTAACAGAATCCGGAGAGACTTAAGACAGATCAAGAGGGATCTCCAGGATATATCAAATCGAGACGTTAATGGACAACGTATTAATGGTGATTCAAGCTCACATGATAGAGACATTTTGAGATGGACTACTCGTGCAGTAGATGCAACAAAGGTTTATGGACTTGATGATGAGGTGTTGTCCATGGAAAACTTGCTTCTCAAGAAACGAAGTGATGTTCATCAGTTCAAGGCAATAGGCATTGTCGGCAGGGATGGTATAGGAAAGACAACGCTTTGCCAACTGATGTTCAACAAAGATGAAGTGAAAAACAACTTCAATCCAAGGATCTGGGTGTGCATGGCCAGACAGCCGGGTGAAGACGAGGATGAGGATCTTAAAGTAGCAATTGTCAAAAGAATGTTGAGGTACCTTGGAGTCGAAGAGGCAATAGTCAAGTCGGTTTGTGATGTAAAACCTCGCCTAGAAGGACTGCTATGTGCTCTTTATCTGCAATTGTTGGGCAAGAGGTATCTAATTGTGCTTGATGATGCTAGGGAGACAGACTCATGGTATAAAGAGTTGGATTCCAGTTTGACTCATGATAAGAAATGGGGTGACAGTTTCGCATTTGGATTTCCAAAAGGCAGTGGAGGAAGAGTCATAGTTACAAGCAGAAATGAAGACATAGCAAATACGATGGTTGGGAAGGAAAATGTACATCACCTTGAGCCCATGTTGGACACTGAAAGCTGCTGGAAAATATTCAAAGACTCGGTTGACAAAGATCTGTTACTTACCATTCCCTCAAACTTTAGATCAGAAGTAAAACAAAAATGTGGTGGCCTTCCGTTAGCTGCAAAAATGATGGGACGTGCAATGCAGGAGGAAGAAGAGCGTACTAGAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

387

Amino Acids

44.56

Weight (kDa)

8.05

Isoelectric Point (pI)

38.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 163 - 344 5.4e-23 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009951)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19060 AT5G45440 AT5G45490 AT5G45490
fragaria_vesca FvH4_6g22150
malus_domestica MD12G1059800.v1.1 MD14G1055400.v1.1
prunus_persica Prupe.7G078500_v2.0.a1 Prupe.7G078700_v2.0.a1
pyrus_communis pycom14g04570
rosa_chinensis RchiOBHm_Chr3g0479501
rosa_laevigata RLG00000023564
rosa_multiflora Rmu_sc0004732.1_g000011
rosa_roxburghii Rroxscaffold_6G00402200
rosa_rugosa Rorug03G0175800 Rorug03G0175900
rosa_samantha Rh3AG226800 Rh3BG259900 Rh3CG256600 Rh3DG252400
rosa_wichuraiana Rw3G020260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 681
AccB1I GGYRCC 1 cut(s) 681
AccIII TCCGGA 2 cut(s) 256, 285
AclWI GGATC 3 cut(s) 321, 608, 654
AcoI YGGCCR 1 cut(s) 615
AfaI GTAC 4 cut(s) 89, 683, 972, 1153
AfiI CCNNNNNNNGG 1 cut(s) 180
AflII CTTAAG 1 cut(s) 295
AflIII ACRYGT 1 cut(s) 126
AgsI TTSAA 7 cut(s) 101, 250, 369, 502, 565, 592, 1021
AjiI CACGTC 1 cut(s) 1127
AjnI CCWGG 1 cut(s) 318
AluBI AGCT 7 cut(s) 135, 190, 265, 372, 1005, 1109, 1161
AluI AGCT 7 cut(s) 135, 190, 265, 372, 1005, 1109, 1161
Alw21I GWGCWC 1 cut(s) 757
Alw26I GTCTC 4 cut(s) 285, 330, 378, 806
AlwI GGATC 3 cut(s) 321, 608, 654
Aor13HI TCCGGA 2 cut(s) 256, 285
AoxI GGCC 3 cut(s) 46, 615, 1096
ApeKI GCWGC 2 cut(s) 1005, 1109
AseI ATTAAT 1 cut(s) 357
Asp700I GAANNNNTTC 1 cut(s) 1016
Asp718I GGTACC 1 cut(s) 681
AsuC2I CCSGG 1 cut(s) 627
AsuHPI GGTGA 4 cut(s) 374, 641, 881, 968
BalI TGGCCA 1 cut(s) 617
BanI GGYRCC 1 cut(s) 681
BanII GRGCYC 1 cut(s) 987
BauI CACGAG 1 cut(s) 405
BbsI GAAGAC 3 cut(s) 129, 639, 942
Bbv12I GWGCWC 1 cut(s) 757
BbvI GCAGC 2 cut(s) 992, 1096
BccI CCATC 4 cut(s) 390, 521, 946, 1114
BceAI ACGGC 1 cut(s) 61
BciT130I CCWGG 1 cut(s) 320
BcnI CCSGG 1 cut(s) 627
BcoDI GTCTC 4 cut(s) 285, 330, 378, 806
BfaI CTAG 5 cut(s) 119, 737, 807, 1155, 1162
BfrI CTTAAG 1 cut(s) 295
BglII AGATCT 1 cut(s) 1039
BisI GCNGC 2 cut(s) 1006, 1110
BlsI GCNGC 2 cut(s) 1007, 1111
Bme1390I CCNGG 2 cut(s) 320, 627
BmgBI CACGTC 1 cut(s) 1127
BmiI GGNNCC 1 cut(s) 683
BmrFI CCNGG 2 cut(s) 320, 627
BmsI GCATC 4 cut(s) 78, 216, 406, 793
BpiI GAAGAC 3 cut(s) 129, 639, 942
BplI GAGNNNNNCTC 2 cut(s) 803, 835
BpmI CTGGAG 1 cut(s) 302
BpuEI CTTGAG 3 cut(s) 217, 458, 1001
BpuMI CCSGG 1 cut(s) 627
BsaBI GATNNNNATC 2 cut(s) 492, 645
BsaJI CCNNGG 4 cut(s) 83, 456, 596, 685
BsaWI WCCGGW 2 cut(s) 256, 285
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 897, 927
Bsc4I CCNNNNNNNGG 1 cut(s) 180
Bse1I ACTGG 1 cut(s) 843
Bse3DI GCAATG 1 cut(s) 1137
Bse8I GATNNNNATC 2 cut(s) 492, 645
BseAI TCCGGA 2 cut(s) 256, 285
BseBI CCWGG 1 cut(s) 320
BseDI CCNNGG 4 cut(s) 83, 456, 596, 685
BseGI GGATG 3 cut(s) 137, 532, 646
BseJI GATNNNNATC 2 cut(s) 492, 645
BseLI CCNNNNNNNGG 1 cut(s) 180
BseMI GCAATG 1 cut(s) 1137
BseNI ACTGG 1 cut(s) 843
BseRI GAGGAG 1 cut(s) 275
BseXI GCAGC 2 cut(s) 992, 1096
BsgI GTGCAG 2 cut(s) 5, 429
BshFI GGCC 3 cut(s) 48, 617, 1098
BshNI GGYRCC 1 cut(s) 681
BsiHKAI GWGCWC 1 cut(s) 757
BsiSI CCGG 3 cut(s) 257, 286, 626
BslFI GGGAC 2 cut(s) 197, 1137
BslI CCNNNNNNNGG 1 cut(s) 180
BsmAI GTCTC 4 cut(s) 285, 330, 378, 806
BsmBI CGTCTC 1 cut(s) 330
BsmFI GGGAC 2 cut(s) 197, 1137
BsnI GGCC 3 cut(s) 48, 617, 1098
Bsp1286I GDGCHC 2 cut(s) 757, 987
Bsp13I TCCGGA 2 cut(s) 256, 285
Bsp1407I TGTACA 1 cut(s) 970
Bsp143I GATC 6 cut(s) 303, 313, 600, 646, 1039, 1070
Bsp19I CCATGG 1 cut(s) 456
BspANI GGCC 3 cut(s) 48, 617, 1098
BspEI TCCGGA 2 cut(s) 256, 285
BspHI TCATGA 1 cut(s) 853
BspLI GGNNCC 1 cut(s) 683
BspPI GGATC 3 cut(s) 321, 608, 654
BspQI GCTCTTC 1 cut(s) 1140
BspT107I GGYRCC 1 cut(s) 681
BspTI CTTAAG 1 cut(s) 295
BsrDI GCAATG 1 cut(s) 1137
BsrGI TGTACA 1 cut(s) 970
BsrI ACTGG 1 cut(s) 843
BssECI CCNNGG 4 cut(s) 83, 456, 596, 685
BssMI GATC 6 cut(s) 303, 313, 600, 646, 1039, 1070
BssSI CACGAG 1 cut(s) 405
BssT1I CCWWGG 4 cut(s) 83, 456, 596, 685
Bst2BI CACGAG 1 cut(s) 405
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 2 cut(s) 154, 875
Bst6I CTCTTC 3 cut(s) 690, 904, 1140
BstAFI CTTAAG 1 cut(s) 295
BstAPI GCANNNNNTGC 1 cut(s) 416
BstAUI TGTACA 1 cut(s) 970
BstC8I GCNNGC 1 cut(s) 147
BstDSI CCRYGG 1 cut(s) 456
BstF5I GGATG 3 cut(s) 137, 532, 646
BstKTI GATC 6 cut(s) 306, 316, 603, 649, 1042, 1073
BstMAI GTCTC 4 cut(s) 285, 330, 378, 806
BstMBI GATC 6 cut(s) 303, 313, 600, 646, 1039, 1070
BstMWI GCNNNNNNNGC 3 cut(s) 196, 416, 519
BstNI CCWGG 1 cut(s) 320
BstNSI RCATGY 1 cut(s) 130
BstSCI CCNGG 2 cut(s) 318, 625
BstV1I GCAGC 2 cut(s) 992, 1096
BstV2I GAAGAC 3 cut(s) 129, 639, 942
BstX2I RGATCY 4 cut(s) 313, 600, 646, 1039
BstXI CCANNNNNNTGG 1 cut(s) 603
BstYI RGATCY 4 cut(s) 313, 600, 646, 1039
BsuRI GGCC 3 cut(s) 48, 617, 1098
BtgI CCRYGG 1 cut(s) 456
BtrI CACGTC 1 cut(s) 1127
BtsCI GGATG 3 cut(s) 137, 532, 646
BtsI GCAGTG 1 cut(s) 907
BtsIMutI CAGTG 2 cut(s) 907, 996
Cac8I GCNNGC 1 cut(s) 147
CciI TCATGA 1 cut(s) 853
Csp6I GTAC 4 cut(s) 88, 682, 971, 1152
CviAII CATG 9 cut(s) 40, 127, 242, 377, 457, 613, 822, 854, 988
CviQI GTAC 4 cut(s) 88, 682, 971, 1152
DpnI GATC 6 cut(s) 305, 315, 602, 648, 1041, 1072
DpnII GATC 6 cut(s) 303, 313, 600, 646, 1039, 1070
EaeI YGGCCR 1 cut(s) 615
Eam1104I CTCTTC 3 cut(s) 690, 904, 1140
EarI CTCTTC 3 cut(s) 690, 904, 1140
Eco130I CCWWGG 4 cut(s) 83, 456, 596, 685
Eco24I GRGCYC 1 cut(s) 987
EcoRII CCWGG 1 cut(s) 318
EcoT14I CCWWGG 4 cut(s) 83, 456, 596, 685
EcoT38I GRGCYC 1 cut(s) 987
ErhI CCWWGG 4 cut(s) 83, 456, 596, 685
Esp3I CGTCTC 1 cut(s) 330
FaeI CATG 9 cut(s) 43, 130, 245, 380, 460, 616, 825, 857, 991
FaqI GGGAC 2 cut(s) 197, 1137
FatI CATG 9 cut(s) 39, 126, 241, 376, 456, 612, 821, 853, 987
Fnu4HI GCNGC 2 cut(s) 1006, 1110
FokI GGATG 3 cut(s) 124, 539, 653
FriOI GRGCYC 1 cut(s) 987
Fsp4HI GCNGC 2 cut(s) 1006, 1110
FspBI CTAG 5 cut(s) 119, 737, 807, 1155, 1162
GluI GCNGC 2 cut(s) 1006, 1110
GsuI CTGGAG 1 cut(s) 302
HaeIII GGCC 3 cut(s) 48, 617, 1098
HapII CCGG 3 cut(s) 257, 286, 626
Hin1II CATG 9 cut(s) 43, 130, 245, 380, 460, 616, 825, 857, 991
HincII GTYRAC 1 cut(s) 1033
HindII GTYRAC 1 cut(s) 1033
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 9 cut(s) 79, 282, 365, 690, 818, 839, 850, 912, 1025
HpaII CCGG 3 cut(s) 257, 286, 626
HphI GGTGA 4 cut(s) 374, 641, 881, 968
Hpy166II GTNNAC 1 cut(s) 1033
Hpy188I TCNGA 2 cut(s) 165, 1075
Hpy188III TCNNGA 9 cut(s) 8, 101, 234, 257, 286, 307, 335, 475, 854
Hpy8I GTNNAC 1 cut(s) 1033
HpyAV CCTTC 4 cut(s) 52, 734, 955, 1109
HpyCH4III ACNGT 2 cut(s) 154, 875
HpyCH4IV ACGT 3 cut(s) 339, 352, 1126
HpyF10VI GCNNNNNNNGC 3 cut(s) 196, 416, 519
HpySE526I ACGT 3 cut(s) 339, 352, 1126
Hsp92II CATG 9 cut(s) 43, 130, 245, 380, 460, 616, 825, 857, 991
Kpn2I TCCGGA 2 cut(s) 256, 285
KpnI GGTACC 1 cut(s) 685
Kzo9I GATC 6 cut(s) 303, 313, 600, 646, 1039, 1070
LguI GCTCTTC 1 cut(s) 1140
LmnI GCTCC 1 cut(s) 262
Lsp1109I GCAGC 2 cut(s) 992, 1096
LweI GCATC 4 cut(s) 78, 216, 406, 793
MaeI CTAG 5 cut(s) 119, 737, 807, 1155, 1162
MaeII ACGT 3 cut(s) 339, 352, 1126
MaeIII GTNAC 3 cut(s) 869, 919, 1044
MalI GATC 6 cut(s) 305, 315, 602, 648, 1041, 1072
MboI GATC 6 cut(s) 303, 313, 600, 646, 1039, 1070
MboII GAAGA 7 cut(s) 134, 644, 707, 921, 947, 1154, 1157
MfeI CAATTG 2 cut(s) 660, 767
MflI RGATCY 4 cut(s) 313, 600, 646, 1039
MhlI GDGCHC 2 cut(s) 757, 987
MlsI TGGCCA 1 cut(s) 617
MluCI AATT 6 cut(s) 95, 175, 267, 660, 767, 789
MluNI TGGCCA 1 cut(s) 617
MlyI GAGTC 5 cut(s) 699, 812, 844, 921, 1019
MmeI TCCRAC 2 cut(s) 816, 972
Mox20I TGGCCA 1 cut(s) 617
MroI TCCGGA 2 cut(s) 256, 285
MroXI GAANNNNTTC 1 cut(s) 1016
MscI TGGCCA 1 cut(s) 617
MseI TTAA 5 cut(s) 276, 296, 342, 357, 651
Msp20I TGGCCA 1 cut(s) 617
MspCI CTTAAG 1 cut(s) 295
MspI CCGG 3 cut(s) 257, 286, 626
MspR9I CCNGG 2 cut(s) 320, 627
MunI CAATTG 2 cut(s) 660, 767
MvaI CCWGG 1 cut(s) 320
MwoI GCNNNNNNNGC 3 cut(s) 196, 416, 519
NciI CCSGG 1 cut(s) 627
NcoI CCATGG 1 cut(s) 456
NdeII GATC 6 cut(s) 303, 313, 600, 646, 1039, 1070
NlaIII CATG 9 cut(s) 43, 130, 245, 380, 460, 616, 825, 857, 991
NlaIV GGNNCC 1 cut(s) 683
NmuCI GTSAC 1 cut(s) 869
NspI RCATGY 1 cut(s) 130
PagI TCATGA 1 cut(s) 853
PciI ACATGT 1 cut(s) 126
PciSI GCTCTTC 1 cut(s) 1140
PdmI GAANNNNTTC 1 cut(s) 1016
PfeI GAWTC 4 cut(s) 79, 282, 365, 839
PfoI TCCNGGA 1 cut(s) 318
PkrI GCNGC 2 cut(s) 1007, 1111
PleI GAGTC 5 cut(s) 698, 812, 844, 920, 1019
PpsI GAGTC 5 cut(s) 698, 812, 844, 920, 1019
PscI ACATGT 1 cut(s) 126
PshBI ATTAAT 1 cut(s) 357
Psp6I CCWGG 1 cut(s) 318
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 1 cut(s) 683
PsuI RGATCY 4 cut(s) 313, 600, 646, 1039
RsaI GTAC 4 cut(s) 89, 683, 972, 1153
RsaNI GTAC 4 cut(s) 88, 682, 971, 1152
SapI GCTCTTC 1 cut(s) 1140
SaqAI TTAA 5 cut(s) 276, 296, 342, 357, 651
SatI GCNGC 2 cut(s) 1006, 1110
Sau3AI GATC 6 cut(s) 303, 313, 600, 646, 1039, 1070
SchI GAGTC 5 cut(s) 699, 812, 844, 921, 1019
ScrFI CCNGG 2 cut(s) 320, 627
SduI GDGCHC 2 cut(s) 757, 987
SfaNI GCATC 4 cut(s) 78, 216, 406, 793
SmlI CTYRAG 5 cut(s) 6, 232, 295, 473, 980
SmoI CTYRAG 5 cut(s) 6, 232, 295, 473, 980
Sse9I AATT 6 cut(s) 95, 175, 267, 660, 767, 789
SspI AATATT 1 cut(s) 1017
SspMI CTAG 5 cut(s) 119, 737, 807, 1155, 1162
StyD4I CCNGG 2 cut(s) 318, 625
StyI CCWWGG 4 cut(s) 83, 456, 596, 685
TaaI ACNGT 2 cut(s) 154, 875
TaiI ACGT 3 cut(s) 342, 355, 1129
TaqI TCGA 2 cut(s) 334, 693
TaqII GACCGA 1 cut(s) 230
TasI AATT 6 cut(s) 95, 175, 267, 660, 767, 789
TatI WGTACW 1 cut(s) 970
TfiI GAWTC 4 cut(s) 79, 282, 365, 839
Tru1I TTAA 5 cut(s) 276, 296, 342, 357, 651
Tru9I TTAA 5 cut(s) 276, 296, 342, 357, 651
TscAI CASTG 2 cut(s) 907, 1003
TseFI GTSAC 1 cut(s) 869
TseI GCWGC 2 cut(s) 1005, 1109
Tsp45I GTSAC 1 cut(s) 869
TspDTI ATGAA 5 cut(s) 28, 71, 482, 588, 948
TspGWI ACGGA 1 cut(s) 1092
TspRI CASTG 2 cut(s) 907, 1003
Vha464I CTTAAG 1 cut(s) 295
VspI ATTAAT 1 cut(s) 357
XceI RCATGY 1 cut(s) 130
XmnI GAANNNNTTC 1 cut(s) 1016
XspI CTAG 5 cut(s) 119, 737, 807, 1155, 1162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.