MD14G1056500.v1.1

DnaJ molecular chaperone homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009153
Physical Location & Seq
Reverse (-)
5836421 .. 5841775
5355 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1056500.v1.1.491

Sequence Viewer

Length: 759 bp
ATGAACACCGCCATGAAAGCGGCTCTCACAAACTCCAAACTCAACTGCTGCATTTATGTGAGGGCCGCCCTCTTTCATTCCACCCCCGTCTTGGATCGTAAGCGACGCTCCTACTGGCAACCTAGATTCAACAACCATGGAAGAAGGTCCAGAAGGTTCCATGGAAAGCAAGCATTACTGAGGAATGTCAATGCTTATGCAGACTTCTTATTTCAGAGTTGGCAGAATGAGTACGATGAGGAGGAGGAGCCATCATCAAGTAGAGGCACCTCATGGTTTAAAAAACAATACTCAGCCAAGGGGTCCAAAAAGAAGTCAGCCGATCAAGGAACCTGGAATTACGGAAGAAATGATTTCCGGTTTTGTGAAGAAGATGAAGACGTGGAGACCATTTTCCGATCCATGTTTGGTGGAAACAAATTTTATTACTGGTCATTCATTAATGAAGAGAATTCACAGCGGAGGAGCTCCAGTAATTACTCTAACTATGGGAGGTCTTGGAATTGGAGACATAACAATAAGGAAGAGTATCAATATGACTCTGAGCCTGAGAATTTGGAGTCAGATGAAAGGTTGGCCCTCGGGTTGCATGCTGCGGGCCCTCTGACACTTGATGATGTCAAGAATGCATATCGAATCTGTGCGCTGAAATGGCATCCAGACCGTCACCAAGGTTCTTCCAAGGCGTCTGCAGAGGAAAAGTTCAAGCTTTGCAGCGCGGCATATCAATCATTATGCGGTAAATTGGCCGTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

253

Amino Acids

29.55

Weight (kDa)

9.11

Isoelectric Point (pI)

57.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 203 - 246 3.4e-10 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 266
AccII CGCG 1 cut(s) 719
AciI CCGC 7 cut(s) 9, 20, 66, 460, 596, 719, 738
AclWI GGATC 2 cut(s) 102, 393
AcoI YGGCCR 1 cut(s) 747
AcsI RAATTY 3 cut(s) 419, 451, 553
AcyI GRCGYC 1 cut(s) 686
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 1 cut(s) 91
AgsI TTSAA 2 cut(s) 130, 706
AjiI CACGTC 1 cut(s) 382
AjnI CCWGG 1 cut(s) 332
AluBI AGCT 2 cut(s) 468, 709
AluI AGCT 2 cut(s) 468, 709
Alw21I GWGCWC 1 cut(s) 470
Alw26I GTCTC 2 cut(s) 380, 502
AlwI GGATC 2 cut(s) 102, 393
Ama87I CYCGRG 1 cut(s) 581
AoxI GGCC 4 cut(s) 63, 576, 598, 747
ApaI GGGCCC 1 cut(s) 602
ApeKI GCWGC 3 cut(s) 48, 593, 714
ApoI RAATTY 3 cut(s) 419, 451, 553
AseI ATTAAT 1 cut(s) 441
AspLEI GCGC 2 cut(s) 646, 719
AspS9I GGNCC 6 cut(s) 63, 147, 303, 577, 598, 599
AsuHPI GGTGA 1 cut(s) 659
AvaI CYCGRG 1 cut(s) 581
AvaII GGWCC 2 cut(s) 147, 303
BaeGI GKGCMC 1 cut(s) 602
BanI GGYRCC 1 cut(s) 266
BanII GRGCYC 2 cut(s) 470, 602
BbsI GAAGAC 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 470
BbvI GCAGC 3 cut(s) 35, 580, 726
BccI CCATC 1 cut(s) 259
BceAI ACGGC 1 cut(s) 734
BciT130I CCWGG 1 cut(s) 334
BcoDI GTCTC 2 cut(s) 380, 502
BfaI CTAG 1 cut(s) 123
BfmI CTRYAG 1 cut(s) 690
BisI GCNGC 6 cut(s) 21, 49, 66, 594, 715, 720
BlsI GCNGC 6 cut(s) 22, 50, 67, 595, 716, 721
Bme1390I CCNGG 1 cut(s) 334
Bme18I GGWCC 2 cut(s) 147, 303
BmeT110I CYCGRG 1 cut(s) 581
BmgBI CACGTC 1 cut(s) 382
BmgT120I GGNCC 6 cut(s) 63, 147, 303, 577, 598, 599
BmiI GGNNCC 6 cut(s) 158, 249, 268, 304, 331, 600
BmrFI CCNGG 1 cut(s) 334
BmsI GCATC 1 cut(s) 664
BpiI GAAGAC 1 cut(s) 384
BpmI CTGGAG 1 cut(s) 454
BsaHI GRCGYC 1 cut(s) 686
BsaI GGTCTC 1 cut(s) 380
BsaJI CCNNGG 6 cut(s) 136, 160, 297, 580, 670, 681
BsaWI WCCGGW 1 cut(s) 357
BsaXI ACNNNNNCTCC 2 cut(s) 457, 487
Bsc4I CCNNNNNNNGG 1 cut(s) 91
Bse1I ACTGG 3 cut(s) 119, 434, 471
BseBI CCWGG 1 cut(s) 334
BseDI CCNNGG 6 cut(s) 136, 160, 297, 580, 670, 681
BseGI GGATG 1 cut(s) 655
BseLI CCNNNNNNNGG 1 cut(s) 91
BseMII CTCAG 4 cut(s) 170, 306, 534, 540
BseNI ACTGG 3 cut(s) 119, 434, 471
BseRI GAGGAG 4 cut(s) 254, 257, 260, 478
BseSI GKGCMC 1 cut(s) 602
BseXI GCAGC 3 cut(s) 35, 580, 726
Bsh1236I CGCG 1 cut(s) 719
BshFI GGCC 4 cut(s) 65, 578, 600, 749
BshNI GGYRCC 1 cut(s) 266
BsiHKAI GWGCWC 1 cut(s) 470
BsiHKCI CYCGRG 1 cut(s) 581
BsiSI CCGG 1 cut(s) 358
BslI CCNNNNNNNGG 1 cut(s) 91
BsmAI GTCTC 2 cut(s) 380, 502
BsmI GAATGC 1 cut(s) 631
BsnI GGCC 4 cut(s) 65, 578, 600, 749
Bso31I GGTCTC 1 cut(s) 380
BsoBI CYCGRG 1 cut(s) 581
Bsp120I GGGCCC 1 cut(s) 598
Bsp1286I GDGCHC 2 cut(s) 470, 602
Bsp143I GATC 3 cut(s) 94, 322, 398
Bsp19I CCATGG 2 cut(s) 136, 160
BspACI CCGC 7 cut(s) 9, 20, 66, 460, 596, 719, 738
BspANI GGCC 4 cut(s) 65, 578, 600, 749
BspCNI CTCAG 4 cut(s) 171, 305, 535, 541
BspFNI CGCG 1 cut(s) 719
BspLI GGNNCC 6 cut(s) 158, 249, 268, 304, 331, 600
BspMAI CTGCAG 1 cut(s) 694
BspPI GGATC 2 cut(s) 102, 393
BspT107I GGYRCC 1 cut(s) 266
BspTNI GGTCTC 1 cut(s) 380
BsrI ACTGG 3 cut(s) 119, 434, 471
BssECI CCNNGG 6 cut(s) 136, 160, 297, 580, 670, 681
BssMI GATC 3 cut(s) 94, 322, 398
BssNI GRCGYC 1 cut(s) 686
BssT1I CCWWGG 5 cut(s) 136, 160, 297, 670, 681
Bst2UI CCWGG 1 cut(s) 334
Bst4CI ACNGT 1 cut(s) 665
Bst6I CTCTTC 2 cut(s) 441, 519
BstACI GRCGYC 1 cut(s) 686
BstC8I GCNNGC 3 cut(s) 171, 591, 598
BstDEI CTNAG 4 cut(s) 179, 292, 543, 549
BstDSI CCRYGG 2 cut(s) 136, 160
BstF5I GGATG 1 cut(s) 655
BstFNI CGCG 1 cut(s) 719
BstHHI GCGC 2 cut(s) 646, 719
BstKTI GATC 3 cut(s) 97, 325, 401
BstMAI GTCTC 2 cut(s) 380, 502
BstMBI GATC 3 cut(s) 94, 322, 398
BstMWI GCNNNNNNNGC 2 cut(s) 17, 652
BstNI CCWGG 1 cut(s) 334
BstNSI RCATGY 1 cut(s) 593
BstSCI CCNGG 1 cut(s) 332
BstSFI CTRYAG 1 cut(s) 690
BstSLI GKGCMC 1 cut(s) 602
BstUI CGCG 1 cut(s) 719
BstV1I GCAGC 3 cut(s) 35, 580, 726
BstV2I GAAGAC 1 cut(s) 384
BsuRI GGCC 4 cut(s) 65, 578, 600, 749
BtgI CCRYGG 2 cut(s) 136, 160
BtrI CACGTC 1 cut(s) 382
BtsCI GGATG 1 cut(s) 655
Cac8I GCNNGC 3 cut(s) 171, 591, 598
CfoI GCGC 2 cut(s) 646, 719
Cfr13I GGNCC 6 cut(s) 63, 147, 303, 577, 598, 599
CseI GACGC 2 cut(s) 114, 675
Csp6I GTAC 1 cut(s) 232
CviAII CATG 6 cut(s) 13, 137, 161, 273, 403, 590
CviQI GTAC 1 cut(s) 232
DdeI CTNAG 4 cut(s) 179, 292, 543, 549
DpnI GATC 3 cut(s) 96, 324, 400
DpnII GATC 3 cut(s) 94, 322, 398
DraI TTTAAA 1 cut(s) 280
EaeI YGGCCR 1 cut(s) 747
Eam1104I CTCTTC 2 cut(s) 441, 519
EarI CTCTTC 2 cut(s) 441, 519
Ecl136II GAGCTC 1 cut(s) 468
Eco130I CCWWGG 5 cut(s) 136, 160, 297, 670, 681
Eco24I GRGCYC 2 cut(s) 470, 602
Eco31I GGTCTC 1 cut(s) 380
Eco47I GGWCC 2 cut(s) 147, 303
Eco53kI GAGCTC 1 cut(s) 468
Eco88I CYCGRG 1 cut(s) 581
EcoICRI GAGCTC 1 cut(s) 468
EcoO109I RGGNCCY 1 cut(s) 599
EcoRI GAATTC 1 cut(s) 451
EcoRII CCWGG 1 cut(s) 332
EcoT14I CCWWGG 5 cut(s) 136, 160, 297, 670, 681
EcoT22I ATGCAT 1 cut(s) 631
EcoT38I GRGCYC 2 cut(s) 470, 602
ErhI CCWWGG 5 cut(s) 136, 160, 297, 670, 681
FaeI CATG 6 cut(s) 16, 140, 164, 276, 406, 593
FatI CATG 6 cut(s) 12, 136, 160, 272, 402, 589
FauI CCCGC 1 cut(s) 589
Fnu4HI GCNGC 6 cut(s) 21, 49, 66, 594, 715, 720
FokI GGATG 1 cut(s) 642
FriOI GRGCYC 2 cut(s) 470, 602
Fsp4HI GCNGC 6 cut(s) 21, 49, 66, 594, 715, 720
FspBI CTAG 1 cut(s) 123
GlaI GCGC 2 cut(s) 645, 718
GluI GCNGC 6 cut(s) 21, 49, 66, 594, 715, 720
GsuI CTGGAG 1 cut(s) 454
HaeIII GGCC 4 cut(s) 65, 578, 600, 749
HapII CCGG 1 cut(s) 358
HgaI GACGC 2 cut(s) 114, 675
HhaI GCGC 2 cut(s) 646, 719
Hin1I GRCGYC 1 cut(s) 686
Hin1II CATG 6 cut(s) 16, 140, 164, 276, 406, 593
Hin6I GCGC 2 cut(s) 644, 717
HinP1I GCGC 2 cut(s) 644, 717
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 4 cut(s) 126, 539, 560, 636
HpaII CCGG 1 cut(s) 358
HphI GGTGA 1 cut(s) 659
Hpy166II GTNNAC 1 cut(s) 754
Hpy188I TCNGA 5 cut(s) 216, 398, 544, 565, 606
Hpy188III TCNNGA 3 cut(s) 150, 622, 659
Hpy8I GTNNAC 1 cut(s) 754
Hpy99I CGWCG 1 cut(s) 108
HpyAV CCTTC 2 cut(s) 138, 147
HpyCH4III ACNGT 1 cut(s) 665
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 6 cut(s) 51, 200, 589, 629, 692, 714
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 652
HpyF3I CTNAG 4 cut(s) 179, 292, 543, 549
HpySE526I ACGT 1 cut(s) 381
Hsp92I GRCGYC 1 cut(s) 686
Hsp92II CATG 6 cut(s) 16, 140, 164, 276, 406, 593
HspAI GCGC 2 cut(s) 644, 717
Kzo9I GATC 3 cut(s) 94, 322, 398
LmnI GCTCC 4 cut(s) 113, 247, 465, 473
LpnPI CCDG 9 cut(s) 100, 163, 319, 346, 371, 415, 484, 561, 672
Lsp1109I GCAGC 3 cut(s) 35, 580, 726
LweI GCATC 1 cut(s) 664
MaeI CTAG 1 cut(s) 123
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 1 cut(s) 665
MalI GATC 3 cut(s) 96, 324, 400
MboI GATC 3 cut(s) 94, 322, 398
MboII GAAGA 8 cut(s) 153, 357, 380, 383, 389, 458, 536, 669
MhlI GDGCHC 2 cut(s) 470, 602
MluCI AATT 7 cut(s) 337, 419, 451, 475, 502, 553, 743
MlyI GAGTC 2 cut(s) 533, 569
Mph1103I ATGCAT 1 cut(s) 631
MseI TTAA 2 cut(s) 279, 441
MslI CAYNNNNRTG 2 cut(s) 11, 56
MspA1I CMGCKG 1 cut(s) 460
MspI CCGG 1 cut(s) 358
MspR9I CCNGG 1 cut(s) 334
Mva1269I GAATGC 1 cut(s) 631
MvaI CCWGG 1 cut(s) 334
MvnI CGCG 1 cut(s) 719
MwoI GCNNNNNNNGC 2 cut(s) 17, 652
NcoI CCATGG 2 cut(s) 136, 160
NdeII GATC 3 cut(s) 94, 322, 398
NlaIII CATG 6 cut(s) 16, 140, 164, 276, 406, 593
NlaIV GGNNCC 6 cut(s) 158, 249, 268, 304, 331, 600
NmuCI GTSAC 1 cut(s) 665
NsiI ATGCAT 1 cut(s) 631
NspI RCATGY 1 cut(s) 593
PaeI GCATGC 1 cut(s) 593
PctI GAATGC 1 cut(s) 631
PfeI GAWTC 2 cut(s) 126, 636
PkrI GCNGC 6 cut(s) 22, 50, 67, 595, 716, 721
PleI GAGTC 2 cut(s) 533, 568
PpsI GAGTC 2 cut(s) 533, 568
PshBI ATTAAT 1 cut(s) 441
Psp124BI GAGCTC 1 cut(s) 470
Psp6I CCWGG 1 cut(s) 332
PspGI CCWGG 1 cut(s) 332
PspN4I GGNNCC 6 cut(s) 158, 249, 268, 304, 331, 600
PspOMI GGGCCC 1 cut(s) 598
PspPI GGNCC 6 cut(s) 63, 147, 303, 577, 598, 599
PstI CTGCAG 1 cut(s) 694
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
RseI CAYNNNNRTG 2 cut(s) 11, 56
SacI GAGCTC 1 cut(s) 470
SaqAI TTAA 2 cut(s) 279, 441
SatI GCNGC 6 cut(s) 21, 49, 66, 594, 715, 720
Sau3AI GATC 3 cut(s) 94, 322, 398
Sau96I GGNCC 6 cut(s) 63, 147, 303, 577, 598, 599
SchI GAGTC 2 cut(s) 533, 569
ScrFI CCNGG 1 cut(s) 334
SduI GDGCHC 2 cut(s) 470, 602
SfaNI GCATC 1 cut(s) 664
SfcI CTRYAG 1 cut(s) 690
SinI GGWCC 2 cut(s) 147, 303
SmiMI CAYNNNNRTG 2 cut(s) 11, 56
SphI GCATGC 1 cut(s) 593
Sse9I AATT 7 cut(s) 337, 419, 451, 475, 502, 553, 743
SsiI CCGC 7 cut(s) 9, 20, 66, 460, 596, 719, 738
SspMI CTAG 1 cut(s) 123
SstI GAGCTC 1 cut(s) 470
StyD4I CCNGG 1 cut(s) 332
StyI CCWWGG 5 cut(s) 136, 160, 297, 670, 681
TaaI ACNGT 1 cut(s) 665
TaiI ACGT 1 cut(s) 384
TaqI TCGA 1 cut(s) 634
TasI AATT 7 cut(s) 337, 419, 451, 475, 502, 553, 743
TauI GCSGC 3 cut(s) 23, 68, 722
TfiI GAWTC 2 cut(s) 126, 636
Tru1I TTAA 2 cut(s) 279, 441
Tru9I TTAA 2 cut(s) 279, 441
TseFI GTSAC 1 cut(s) 665
TseI GCWGC 3 cut(s) 48, 593, 714
Tsp45I GTSAC 1 cut(s) 665
TspDTI ATGAA 7 cut(s) 17, 29, 65, 390, 427, 459, 582
TspGWI ACGGA 1 cut(s) 357
VpaK11BI GGWCC 2 cut(s) 147, 303
VspI ATTAAT 1 cut(s) 441
XapI RAATTY 3 cut(s) 419, 451, 553
XceI RCATGY 1 cut(s) 593
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XspI CTAG 1 cut(s) 123
Zsp2I ATGCAT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.