Rroxscaffold_6G00402150

DnaJ molecular chaperone homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0009153
Physical Location & Seq
Forward (+)
24528936 .. 24533251
4316 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00402150.1

Sequence Viewer

Length: 753 bp
ATGAACAGCGCGATGAAAACGGCTCTCCTAAACTCAAAACTGAACTATGTGAGGAGTGCCCTGTTTCATTCAACCCCCGTGTTGGATCGTAAAAGACGCAACAACAACTGGGAATCATTTAGAAACCACAACTATTCAAAAAGGTCCAGAAGGATACATGCAAAACAAACATTATTGCGCAATGTCAATGATTATGCAGACTTCCTATTTCAGAAATGGCAGGATCCGTATAATTTGAACGAGCCATCTTCAAAAAGAGGCACTTCATGGTTTAAAAAGCAATACTCAGCGAAGGGCTCCAAAAAGAAATGGGGAAGCAATCAAGGAGCCTCCAGCCGGGGCAGAAGAGGTTTCGAGTTTTATGATGAATACGTGGATGTAGAGACCATTTTCCAGTCCAGGTTTGGTGGGAGCGGATTCTTTTACTGGTCATTTGTCAATGAAGATGATTCACAGCGGAGAAGCTCTTCAAATTACTCTAATCACTCTGGGAGATCATGGAGTTGGAGACATCGTAATGAGGAACAATACGAATCTGAGTCTGACGATTCAGAGTCAGATGAGAGGTTGGCCCTTGGATTGAGTGCTGCAGGCCCTCTGAAACTTGAAGATGTCAAAAATGCATATCGAACCTGTGCACTGAAATGGCATCCAGATCGTCACCAGGGCTCTTCCAAGGCTGCTGCAGAGGAAAAGTTCAAGCATTGCAGCGCAGCTTATCAATCTTTATGTGATAAATTGGCCGTGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

250

Amino Acids

29.15

Weight (kDa)

9.62

Isoelectric Point (pI)

58.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 201 - 246 2.7e-11 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 179
AccBSI CCGCTC 1 cut(s) 414
AccII CGCG 1 cut(s) 11
AciI CCGC 2 cut(s) 414, 457
AclWI GGATC 3 cut(s) 93, 218, 231
AcoI YGGCCR 1 cut(s) 741
AfiI CCNNNNNNNGG 2 cut(s) 82, 336
AgsI TTSAA 7 cut(s) 72, 138, 238, 252, 471, 608, 700
AjnI CCWGG 2 cut(s) 398, 663
AluBI AGCT 2 cut(s) 465, 716
AluI AGCT 2 cut(s) 465, 716
Alw21I GWGCWC 1 cut(s) 640
Alw26I GTCTC 2 cut(s) 377, 502
Alw44I GTGCAC 1 cut(s) 636
AlwI GGATC 3 cut(s) 93, 218, 231
AoxI GGCC 3 cut(s) 570, 592, 741
ApaLI GTGCAC 1 cut(s) 636
ApeKI GCWGC 5 cut(s) 587, 680, 683, 708, 713
Asp700I GAANNNNTTC 1 cut(s) 466
AspLEI GCGC 3 cut(s) 11, 180, 713
AspS9I GGNCC 3 cut(s) 144, 571, 593
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 1 cut(s) 653
AvaII GGWCC 1 cut(s) 144
BaeGI GKGCMC 2 cut(s) 61, 640
BamHI GGATCC 1 cut(s) 223
BanII GRGCYC 2 cut(s) 299, 671
Bbv12I GWGCWC 1 cut(s) 640
BbvI GCAGC 5 cut(s) 574, 667, 670, 720, 725
BccI CCATC 1 cut(s) 253
BceAI ACGGC 2 cut(s) 36, 728
BcgI CGANNNNNNTGC 2 cut(s) 638, 672
BciT130I CCWGG 2 cut(s) 400, 665
BciVI GTATCC 1 cut(s) 147
BcnI CCSGG 1 cut(s) 338
BcoDI GTCTC 2 cut(s) 377, 502
BfmI CTRYAG 2 cut(s) 588, 684
BfuI GTATCC 1 cut(s) 147
BisI GCNGC 5 cut(s) 588, 681, 684, 709, 714
BlsI GCNGC 5 cut(s) 589, 682, 685, 710, 715
Bme1390I CCNGG 3 cut(s) 338, 400, 665
Bme18I GGWCC 1 cut(s) 144
BmgT120I GGNCC 3 cut(s) 144, 571, 593
BmiI GGNNCC 3 cut(s) 225, 298, 328
BmrFI CCNGG 3 cut(s) 338, 400, 665
BmrI ACTGGG 1 cut(s) 118
BmsI GCATC 1 cut(s) 658
BmuI ACTGGG 1 cut(s) 118
BpmI CTGGAG 1 cut(s) 316
BpuMI CCSGG 1 cut(s) 338
BsaAI YACGTR 1 cut(s) 373
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 4 cut(s) 337, 574, 664, 675
BsaXI ACNNNNNCTCC 2 cut(s) 499, 529
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 336
Bse1I ACTGG 3 cut(s) 113, 394, 431
Bse3DI GCAATG 2 cut(s) 187, 703
BseBI CCWGG 2 cut(s) 400, 665
BseDI CCNNGG 4 cut(s) 337, 574, 664, 675
BseGI GGATG 2 cut(s) 382, 649
BseLI CCNNNNNNNGG 2 cut(s) 82, 336
BseMI GCAATG 2 cut(s) 187, 703
BseMII CTCAG 2 cut(s) 300, 528
BseNI ACTGG 3 cut(s) 113, 394, 431
BseRI GAGGAG 1 cut(s) 67
BseSI GKGCMC 2 cut(s) 61, 640
BseXI GCAGC 5 cut(s) 574, 667, 670, 720, 725
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 3 cut(s) 572, 594, 743
BsiHKAI GWGCWC 1 cut(s) 640
BsiSI CCGG 1 cut(s) 337
BslI CCNNNNNNNGG 2 cut(s) 82, 336
BsmAI GTCTC 2 cut(s) 377, 502
BsnI GGCC 3 cut(s) 572, 594, 743
Bso31I GGTCTC 1 cut(s) 377
Bsp1286I GDGCHC 4 cut(s) 61, 299, 640, 671
Bsp143I GATC 4 cut(s) 85, 223, 494, 655
BspACI CCGC 2 cut(s) 414, 457
BspANI GGCC 3 cut(s) 572, 594, 743
BspCNI CTCAG 2 cut(s) 299, 529
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 3 cut(s) 225, 298, 328
BspMAI CTGCAG 2 cut(s) 592, 688
BspPI GGATC 3 cut(s) 93, 218, 231
BspQI GCTCTTC 2 cut(s) 472, 676
BspTNI GGTCTC 1 cut(s) 377
BsrBI CCGCTC 1 cut(s) 414
BsrDI GCAATG 2 cut(s) 187, 703
BsrI ACTGG 3 cut(s) 113, 394, 431
BssECI CCNNGG 4 cut(s) 337, 574, 664, 675
BssMI GATC 4 cut(s) 85, 223, 494, 655
BssT1I CCWWGG 2 cut(s) 574, 675
Bst2UI CCWGG 2 cut(s) 400, 665
Bst6I CTCTTC 3 cut(s) 340, 472, 676
BstBAI YACGTR 1 cut(s) 373
BstC8I GCNNGC 1 cut(s) 592
BstDEI CTNAG 2 cut(s) 286, 537
BstF5I GGATG 2 cut(s) 382, 649
BstFNI CGCG 1 cut(s) 11
BstHHI GCGC 3 cut(s) 11, 180, 713
BstKTI GATC 4 cut(s) 88, 226, 497, 658
BstMAI GTCTC 2 cut(s) 377, 502
BstMBI GATC 4 cut(s) 85, 223, 494, 655
BstNI CCWGG 2 cut(s) 400, 665
BstNSI RCATGY 1 cut(s) 161
BstSCI CCNGG 3 cut(s) 336, 398, 663
BstSFI CTRYAG 2 cut(s) 588, 684
BstSLI GKGCMC 2 cut(s) 61, 640
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 5 cut(s) 574, 667, 670, 720, 725
BstX2I RGATCY 1 cut(s) 223
BstYI RGATCY 1 cut(s) 223
BsuI GTATCC 1 cut(s) 147
BsuRI GGCC 3 cut(s) 572, 594, 743
BtgZI GCGATG 1 cut(s) 26
BtsCI GGATG 2 cut(s) 382, 649
BtsIMutI CAGTG 1 cut(s) 638
Cac8I GCNNGC 1 cut(s) 592
CfoI GCGC 3 cut(s) 11, 180, 713
Cfr13I GGNCC 3 cut(s) 144, 571, 593
CseI GACGC 1 cut(s) 105
CviAII CATG 3 cut(s) 158, 267, 498
DdeI CTNAG 2 cut(s) 286, 537
DpnI GATC 4 cut(s) 87, 225, 496, 657
DpnII GATC 4 cut(s) 85, 223, 494, 655
DraI TTTAAA 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 741
Eam1104I CTCTTC 3 cut(s) 340, 472, 676
EarI CTCTTC 3 cut(s) 340, 472, 676
Eco130I CCWWGG 2 cut(s) 574, 675
Eco24I GRGCYC 2 cut(s) 299, 671
Eco31I GGTCTC 1 cut(s) 377
Eco47I GGWCC 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 593
EcoRII CCWGG 2 cut(s) 398, 663
EcoT14I CCWWGG 2 cut(s) 574, 675
EcoT22I ATGCAT 1 cut(s) 625
EcoT38I GRGCYC 2 cut(s) 299, 671
ErhI CCWWGG 2 cut(s) 574, 675
FaeI CATG 3 cut(s) 161, 270, 501
FaiI YATR 9 cut(s) 48, 159, 195, 231, 268, 363, 499, 625, 730
FalI AAGNNNNNCTT 2 cut(s) 247, 279
FatI CATG 3 cut(s) 157, 266, 497
Fnu4HI GCNGC 5 cut(s) 588, 681, 684, 709, 714
FokI GGATG 2 cut(s) 389, 636
FriOI GRGCYC 2 cut(s) 299, 671
Fsp4HI GCNGC 5 cut(s) 588, 681, 684, 709, 714
FspI TGCGCA 1 cut(s) 179
GlaI GCGC 3 cut(s) 10, 179, 712
GluI GCNGC 5 cut(s) 588, 681, 684, 709, 714
GsuI CTGGAG 1 cut(s) 316
HaeIII GGCC 3 cut(s) 572, 594, 743
HapII CCGG 1 cut(s) 337
HgaI GACGC 1 cut(s) 105
HhaI GCGC 3 cut(s) 11, 180, 713
Hin1II CATG 3 cut(s) 161, 270, 501
Hin6I GCGC 3 cut(s) 9, 178, 711
HinP1I GCGC 3 cut(s) 9, 178, 711
HinfI GANTC 7 cut(s) 113, 417, 449, 533, 539, 548, 554
HpaII CCGG 1 cut(s) 337
HphI GGTGA 1 cut(s) 653
Hpy166II GTNNAC 1 cut(s) 638
Hpy188I TCNGA 6 cut(s) 213, 538, 544, 553, 559, 600
Hpy188III TCNNGA 2 cut(s) 147, 653
Hpy8I GTNNAC 1 cut(s) 638
HpyAV CCTTC 2 cut(s) 144, 286
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 7 cut(s) 161, 197, 590, 623, 638, 686, 708
HpyF3I CTNAG 2 cut(s) 286, 537
HpySE526I ACGT 1 cut(s) 372
Hsp92II CATG 3 cut(s) 161, 270, 501
HspAI GCGC 3 cut(s) 9, 178, 711
Kzo9I GATC 4 cut(s) 85, 223, 494, 655
LguI GCTCTTC 2 cut(s) 472, 676
LmnI GCTCC 3 cut(s) 302, 326, 411
Lsp1109I GCAGC 5 cut(s) 574, 667, 670, 720, 725
LweI GCATC 1 cut(s) 658
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 1 cut(s) 659
MalI GATC 4 cut(s) 87, 225, 496, 657
MbiI CCGCTC 1 cut(s) 414
MboI GATC 4 cut(s) 85, 223, 494, 655
MboII GAAGA 6 cut(s) 240, 357, 455, 459, 620, 663
MflI RGATCY 1 cut(s) 223
MhlI GDGCHC 4 cut(s) 61, 299, 640, 671
MluCI AATT 4 cut(s) 232, 472, 737, 748
MlyI GAGTC 2 cut(s) 548, 563
MmeI TCCRAC 2 cut(s) 63, 485
MnlI CCTC 8 cut(s) 45, 251, 340, 341, 514, 558, 606, 682
Mph1103I ATGCAT 1 cut(s) 625
MroXI GAANNNNTTC 1 cut(s) 466
MseI TTAA 1 cut(s) 273
MslI CAYNNNNRTG 2 cut(s) 516, 643
MspA1I CMGCKG 1 cut(s) 457
MspI CCGG 1 cut(s) 337
MspR9I CCNGG 3 cut(s) 338, 400, 665
MvaI CCWGG 2 cut(s) 400, 665
MvnI CGCG 1 cut(s) 11
NciI CCSGG 1 cut(s) 338
NdeII GATC 4 cut(s) 85, 223, 494, 655
NlaIII CATG 3 cut(s) 161, 270, 501
NlaIV GGNNCC 3 cut(s) 225, 298, 328
NmuCI GTSAC 1 cut(s) 659
NsbI TGCGCA 1 cut(s) 179
NsiI ATGCAT 1 cut(s) 625
NspI RCATGY 1 cut(s) 161
PciSI GCTCTTC 2 cut(s) 472, 676
PdmI GAANNNNTTC 1 cut(s) 466
PfeI GAWTC 5 cut(s) 113, 417, 449, 533, 548
PkrI GCNGC 5 cut(s) 589, 682, 685, 710, 715
PleI GAGTC 2 cut(s) 547, 562
PpsI GAGTC 2 cut(s) 547, 562
Ppu21I YACGTR 1 cut(s) 373
Psp6I CCWGG 2 cut(s) 398, 663
PspGI CCWGG 2 cut(s) 398, 663
PspN4I GGNNCC 3 cut(s) 225, 298, 328
PspPI GGNCC 3 cut(s) 144, 571, 593
PstI CTGCAG 2 cut(s) 592, 688
PsuI RGATCY 1 cut(s) 223
RseI CAYNNNNRTG 2 cut(s) 516, 643
SapI GCTCTTC 2 cut(s) 472, 676
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 5 cut(s) 588, 681, 684, 709, 714
Sau3AI GATC 4 cut(s) 85, 223, 494, 655
Sau96I GGNCC 3 cut(s) 144, 571, 593
SchI GAGTC 2 cut(s) 548, 563
ScrFI CCNGG 3 cut(s) 338, 400, 665
SduI GDGCHC 4 cut(s) 61, 299, 640, 671
SetI ASST 8 cut(s) 146, 352, 375, 404, 467, 569, 635, 718
SfaNI GCATC 1 cut(s) 658
SfcI CTRYAG 2 cut(s) 588, 684
SinI GGWCC 1 cut(s) 144
SmiMI CAYNNNNRTG 2 cut(s) 516, 643
Sse9I AATT 4 cut(s) 232, 472, 737, 748
SsiI CCGC 2 cut(s) 414, 457
StyD4I CCNGG 3 cut(s) 336, 398, 663
StyI CCWWGG 2 cut(s) 574, 675
TaiI ACGT 1 cut(s) 375
TaqI TCGA 2 cut(s) 354, 628
TasI AATT 4 cut(s) 232, 472, 737, 748
TfiI GAWTC 5 cut(s) 113, 417, 449, 533, 548
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TscAI CASTG 1 cut(s) 645
TseFI GTSAC 1 cut(s) 659
TseI GCWGC 5 cut(s) 587, 680, 683, 708, 713
Tsp45I GTSAC 1 cut(s) 659
TspDTI ATGAA 6 cut(s) 17, 29, 56, 255, 381, 456
TspGWI ACGGA 1 cut(s) 216
TspRI CASTG 1 cut(s) 645
VneI GTGCAC 1 cut(s) 636
VpaK11BI GGWCC 1 cut(s) 144
XceI RCATGY 1 cut(s) 161
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XmnI GAANNNNTTC 1 cut(s) 466
Zsp2I ATGCAT 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.