MD15G1079300.v1.1

acetyltransferase At3g50280-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002329
Physical Location & Seq
Forward (+)
5414839 .. 5416345
1507 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1079300.v1.1.491

Sequence Viewer

Length: 1380 bp
ATGAGCTCCAAAAATGTCAAACAAATTTCAGAGTGGTTTATCTACCCACACCATGCTTCTGAAGAGTCAAAGAAGCCGTTCTACTTGACACCAAATGATCTGGCGTTGCTTTCTGTACACTATATCCAAAGGGGCCTTCTCTTTACGAAACCACCAGAAGCAATATTAGATCAGAAAAACTTCACCAATTCTCTGTTGGAAAGGCTCAAGCAGTCTCTTTCCATTGCGCTTGTTCATTTCTACCCACTCGCAGGTCGCTTGGTGACCACAAAATCTGAGGACCCGGCTTTGTATTTGGTGTATGTAGATTGCAACAACAGCCCTGGAGCCAAATTCATGTATGCAACTTTGGACATGTCAATCTCTGACATTCTTTCGCCAACTGATGTCCCGTTGGTGGTCCAATCATTTTTTGATCATGACAGGGCAGTCAACCATGATGGACATTTCATGTCTTTGGTGACGATTCAGGTCACGGAACTTGTGGATGGCATCTTCATAGGACTTTCCATGAATCACTGCCTTGGTGATGGAACCTCGTTCTGGCATTTCTTCAACATTTGGTCTGAAATTTTTCAAGCACAAGCACAAGGAGGTCATAGTAATTTTGTAGCCACTATCTCACGCCCACCTGTCCTCAAGCGCTGGTTTCCAGATGGTCATGGTCCAATTATCAGCCTCCCTTTCAAACACTCAGATGAGTTCATTGGCAGATTTGAAGCTCCCCCACTCAGGGAGAGAATGTTTCACTTCACCGCAGAATCCATAGCCAAACTCAAAGCAAAGGCCAATGCGGAGTCTGACACCACCAAAATCTCTTCCTTCCAGGCTTTATCTGCCCTTCTCTGGCGCTCCATTACTCGAGCGCGTTGTCAACCACCTGATCAAGGAACCAGCTGCAGGTTGGCTGCGAATAACAGAGCGAGATTAGACCCGCCACTGTCTGGAGACTATTTCGGGAACTCAATTTACCCCATTAGTTCAAAAATGGTTCCAGCAGGTGAGCTGCTTGAACATGGGCTTGGATGGGCAGCGTGGAAGTTGCACGAGGCGGTGGTTAACCACAATGACAAGGTGATACGAGAGCATACTAATGCGTGGCTGGAGTCTCCCGTTGTGTACCAAGTGAGTGTTTTTGAACCATCGAGTGTGACAATTGGGAGTTCGCCAAGATTCAACATGTATGGAACTGAATTTGGAATGGGAAAAGCGGTAGCACTTCGAAGTGGGTACGCACACAAGTTCAGTGGGAAGGTATCATCGTATGAAGGACGCAAAGAAGGAAGCATAGACACGGAGGTGTGTCTTCTACCTGATGCAATGAGCGCTCTTGAATGTGACAAGGAGTTTATGGAATTTGTCTCTGTATCACATTATTAG

Protein Analysis

460

Amino Acids

51.38

Weight (kDa)

6.34

Isoelectric Point (pI)

45.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 6 - 451 1.7e-67 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 989
AasI GACNNNNNNGTC 1 cut(s) 428
Acc36I ACCTGC 3 cut(s) 242, 891, 989
AccB7I CCANNNNNTGG 1 cut(s) 944
AccII CGCG 1 cut(s) 868
AciI CCGC 5 cut(s) 756, 794, 935, 1052, 1211
AcsI RAATTY 5 cut(s) 24, 332, 570, 1193, 1355
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 3 cut(s) 117, 1121, 1232
AfeI AGCGCT 2 cut(s) 644, 1327
AfiI CCNNNNNNNGG 9 cut(s) 251, 397, 543, 732, 733, 846, 887, 900, 944
AflIII ACRYGT 2 cut(s) 354, 1179
AgsI TTSAA 9 cut(s) 556, 578, 688, 719, 984, 1013, 1139, 1177, 1334
AjnI CCWGG 2 cut(s) 322, 825
AjuI GAANNNNNNNTTGG 4 cut(s) 120, 152, 1005, 1037
AleI CACNNNNGTG 1 cut(s) 1298
AluBI AGCT 4 cut(s) 6, 722, 897, 1006
AluI AGCT 4 cut(s) 6, 722, 897, 1006
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 4 cut(s) 219, 942, 1113, 1366
Ama87I CYCGRG 1 cut(s) 861
Aor51HI AGCGCT 2 cut(s) 644, 1327
AoxI GGCC 2 cut(s) 133, 786
ApeKI GCWGC 4 cut(s) 897, 908, 1006, 1031
ApoI RAATTY 5 cut(s) 24, 332, 570, 1193, 1355
Asp700I GAANNNNTTC 3 cut(s) 77, 179, 573
AspLEI GCGC 5 cut(s) 229, 645, 852, 868, 1328
AspS9I GGNCC 4 cut(s) 133, 280, 400, 665
AsuC2I CCSGG 1 cut(s) 284
AsuHPI GGTGA 7 cut(s) 175, 274, 472, 539, 745, 1013, 1087
AsuII TTCGAA 1 cut(s) 1222
AvaI CYCGRG 1 cut(s) 861
AvaII GGWCC 3 cut(s) 280, 400, 665
BanII GRGCYC 1 cut(s) 8
BarI GAAGNNNNNNTAC 2 cut(s) 65, 97
BauI CACGAG 1 cut(s) 1046
BbsI GAAGAC 1 cut(s) 1298
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 4 cut(s) 884, 895, 993, 1043
BccI CCATC 6 cut(s) 434, 482, 524, 650, 1020, 1150
BceAI ACGGC 1 cut(s) 61
BciT130I CCWGG 2 cut(s) 324, 827
BclI TGATCA 2 cut(s) 415, 883
BcnI CCSGG 1 cut(s) 284
BcoDI GTCTC 4 cut(s) 219, 942, 1113, 1366
BfmI CTRYAG 1 cut(s) 898
BfoI RGCGCY 3 cut(s) 646, 853, 1329
BfuAI ACCTGC 3 cut(s) 242, 891, 989
BisI GCNGC 4 cut(s) 898, 909, 1007, 1032
BlsI GCNGC 4 cut(s) 899, 910, 1008, 1033
Bme1390I CCNGG 3 cut(s) 284, 324, 827
Bme18I GGWCC 3 cut(s) 280, 400, 665
BmeT110I CYCGRG 1 cut(s) 861
BmgT120I GGNCC 4 cut(s) 133, 280, 400, 665
BmiI GGNNCC 6 cut(s) 134, 282, 328, 535, 892, 993
BmrFI CCNGG 3 cut(s) 284, 324, 827
BmsI GCATC 2 cut(s) 501, 1306
BpiI GAAGAC 1 cut(s) 1298
BpmI CTGGAG 3 cut(s) 345, 966, 1124
Bpu14I TTCGAA 1 cut(s) 1222
BpuEI CTTGAG 2 cut(s) 191, 623
BpuMI CCSGG 1 cut(s) 284
BsaJI CCNNGG 2 cut(s) 322, 523
BsaXI ACNNNNNCTCC 2 cut(s) 728, 758
Bsc4I CCNNNNNNNGG 9 cut(s) 251, 397, 543, 732, 733, 846, 887, 900, 944
Bse3DI GCAATG 2 cut(s) 222, 1326
BseBI CCWGG 2 cut(s) 324, 827
BseDI CCNNGG 2 cut(s) 322, 523
BseGI GGATG 2 cut(s) 493, 1031
BseLI CCNNNNNNNGG 9 cut(s) 251, 397, 543, 732, 733, 846, 887, 900, 944
BseMI GCAATG 2 cut(s) 222, 1326
BseMII CTCAG 3 cut(s) 267, 708, 745
BseXI GCAGC 4 cut(s) 884, 895, 993, 1043
Bsh1236I CGCG 1 cut(s) 868
BshFI GGCC 2 cut(s) 135, 788
BsiHKAI GWGCWC 1 cut(s) 8
BsiHKCI CYCGRG 1 cut(s) 861
BsiSI CCGG 1 cut(s) 284
BslFI GGGAC 1 cut(s) 374
BslI CCNNNNNNNGG 9 cut(s) 251, 397, 543, 732, 733, 846, 887, 900, 944
BsmAI GTCTC 4 cut(s) 219, 942, 1113, 1366
BsmFI GGGAC 1 cut(s) 374
BsnI GGCC 2 cut(s) 135, 788
BsoBI CYCGRG 1 cut(s) 861
Bsp119I TTCGAA 1 cut(s) 1222
Bsp1286I GDGCHC 1 cut(s) 8
Bsp1407I TGTACA 1 cut(s) 115
Bsp143I GATC 4 cut(s) 97, 169, 415, 883
BspACI CCGC 5 cut(s) 756, 794, 935, 1052, 1211
BspANI GGCC 2 cut(s) 135, 788
BspCNI CTCAG 3 cut(s) 268, 707, 744
BspFNI CGCG 1 cut(s) 868
BspHI TCATGA 1 cut(s) 418
BspLI GGNNCC 6 cut(s) 134, 282, 328, 535, 892, 993
BspMAI CTGCAG 1 cut(s) 902
BspMI ACCTGC 3 cut(s) 242, 891, 989
BspT104I TTCGAA 1 cut(s) 1222
BsrDI GCAATG 2 cut(s) 222, 1326
BsrGI TGTACA 1 cut(s) 115
BssECI CCNNGG 2 cut(s) 322, 523
BssMI GATC 4 cut(s) 97, 169, 415, 883
BssSI CACGAG 1 cut(s) 1046
BssT1I CCWWGG 1 cut(s) 523
Bst2BI CACGAG 1 cut(s) 1046
Bst2UI CCWGG 2 cut(s) 324, 827
Bst4CI ACNGT 1 cut(s) 942
Bst6I CTCTTC 2 cut(s) 57, 823
BstAUI TGTACA 1 cut(s) 115
BstBI TTCGAA 1 cut(s) 1222
BstDEI CTNAG 3 cut(s) 276, 694, 731
BstEII GGTNACC 1 cut(s) 262
BstENI CCTNNNNNAGG 1 cut(s) 885
BstF5I GGATG 2 cut(s) 493, 1031
BstFNI CGCG 1 cut(s) 868
BstH2I RGCGCY 3 cut(s) 646, 853, 1329
BstHHI GCGC 5 cut(s) 229, 645, 852, 868, 1328
BstKTI GATC 4 cut(s) 100, 172, 418, 886
BstMAI GTCTC 4 cut(s) 219, 942, 1113, 1366
BstMBI GATC 4 cut(s) 97, 169, 415, 883
BstMWI GCNNNNNNNGC 3 cut(s) 318, 836, 1325
BstNI CCWGG 2 cut(s) 324, 827
BstNSI RCATGY 2 cut(s) 358, 1183
BstPI GGTNACC 1 cut(s) 262
BstSCI CCNGG 3 cut(s) 282, 322, 825
BstSFI CTRYAG 1 cut(s) 898
BstUI CGCG 1 cut(s) 868
BstV1I GCAGC 4 cut(s) 884, 895, 993, 1043
BstV2I GAAGAC 1 cut(s) 1298
BsuRI GGCC 2 cut(s) 135, 788
BtsCI GGATG 2 cut(s) 493, 1031
BtsI GCAGTG 1 cut(s) 517
BtsIMutI CAGTG 3 cut(s) 517, 938, 1252
BveI ACCTGC 3 cut(s) 242, 891, 989
CciI TCATGA 1 cut(s) 418
CfoI GCGC 5 cut(s) 229, 645, 852, 868, 1328
Cfr13I GGNCC 4 cut(s) 133, 280, 400, 665
CseI GACGC 1 cut(s) 1281
Csp6I GTAC 3 cut(s) 116, 1120, 1231
CspCI CAANNNNNGTGG 2 cut(s) 1228, 1263
CviQI GTAC 3 cut(s) 116, 1120, 1231
DdeI CTNAG 3 cut(s) 276, 694, 731
DpnI GATC 4 cut(s) 99, 171, 417, 885
DpnII GATC 4 cut(s) 97, 169, 415, 883
DrdI GACNNNNNNGTC 1 cut(s) 428
DseDI GACNNNNNNGTC 1 cut(s) 428
Eam1104I CTCTTC 2 cut(s) 57, 823
EarI CTCTTC 2 cut(s) 57, 823
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 1 cut(s) 523
Eco24I GRGCYC 1 cut(s) 8
Eco47I GGWCC 3 cut(s) 280, 400, 665
Eco47III AGCGCT 2 cut(s) 644, 1327
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 81
Eco88I CYCGRG 1 cut(s) 861
Eco91I GGTNACC 1 cut(s) 262
EcoICRI GAGCTC 1 cut(s) 6
EcoNI CCTNNNNNAGG 1 cut(s) 885
EcoO109I RGGNCCY 2 cut(s) 133, 280
EcoO65I GGTNACC 1 cut(s) 262
EcoRII CCWGG 2 cut(s) 322, 825
EcoT14I CCWWGG 1 cut(s) 523
EcoT38I GRGCYC 1 cut(s) 8
ErhI CCWWGG 1 cut(s) 523
FaqI GGGAC 1 cut(s) 374
FauI CCCGC 1 cut(s) 942
FbaI TGATCA 2 cut(s) 415, 883
Fnu4HI GCNGC 4 cut(s) 898, 909, 1007, 1032
FokI GGATG 2 cut(s) 500, 1038
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 4 cut(s) 898, 909, 1007, 1032
GlaI GCGC 5 cut(s) 228, 644, 851, 867, 1327
GluI GCNGC 4 cut(s) 898, 909, 1007, 1032
GsuI CTGGAG 3 cut(s) 345, 966, 1124
HaeII RGCGCY 3 cut(s) 646, 853, 1329
HaeIII GGCC 2 cut(s) 135, 788
HapII CCGG 1 cut(s) 284
HgaI GACGC 1 cut(s) 1281
HhaI GCGC 5 cut(s) 229, 645, 852, 868, 1328
Hin6I GCGC 5 cut(s) 227, 643, 850, 866, 1326
HinP1I GCGC 5 cut(s) 227, 643, 850, 866, 1326
HincII GTYRAC 3 cut(s) 433, 875, 1060
HindII GTYRAC 3 cut(s) 433, 875, 1060
HinfI GANTC 7 cut(s) 65, 466, 514, 761, 797, 1106, 1173
HpaI GTTAAC 1 cut(s) 1060
HpaII CCGG 1 cut(s) 284
HphI GGTGA 7 cut(s) 175, 274, 472, 539, 745, 1013, 1087
Hpy166II GTNNAC 5 cut(s) 118, 433, 875, 1060, 1120
Hpy188I TCNGA 8 cut(s) 31, 61, 174, 277, 367, 568, 697, 802
Hpy188III TCNNGA 5 cut(s) 419, 653, 945, 958, 1331
Hpy8I GTNNAC 5 cut(s) 118, 433, 875, 1060, 1120
HpyAV CCTTC 6 cut(s) 146, 832, 851, 1246, 1262, 1274
HpyCH4III ACNGT 1 cut(s) 942
HpyCH4V TGCA 5 cut(s) 312, 344, 900, 1045, 1319
HpyF10VI GCNNNNNNNGC 3 cut(s) 318, 836, 1325
HpyF3I CTNAG 3 cut(s) 276, 694, 731
HspAI GCGC 5 cut(s) 227, 643, 850, 866, 1326
Ksp22I TGATCA 2 cut(s) 415, 883
KspAI GTTAAC 1 cut(s) 1060
Kzo9I GATC 4 cut(s) 97, 169, 415, 883
LmnI GCTCC 4 cut(s) 11, 326, 727, 857
Lsp1109I GCAGC 4 cut(s) 884, 895, 993, 1043
LweI GCATC 2 cut(s) 501, 1306
MaeIII GTNAC 5 cut(s) 262, 460, 472, 1150, 1337
MalI GATC 4 cut(s) 99, 171, 417, 885
MboI GATC 4 cut(s) 97, 169, 415, 883
MboII GAAGA 5 cut(s) 74, 487, 544, 810, 1298
MfeI CAATTG 1 cut(s) 1155
MhlI GDGCHC 1 cut(s) 8
MlyI GAGTC 3 cut(s) 74, 806, 1115
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 7 cut(s) 271, 547, 587, 647, 689, 1042, 1291
MroXI GAANNNNTTC 3 cut(s) 77, 179, 573
MseI TTAA 1 cut(s) 1059
MslI CAYNNNNRTG 3 cut(s) 696, 1092, 1298
MspA1I CMGCKG 1 cut(s) 897
MspI CCGG 1 cut(s) 284
MspR9I CCNGG 3 cut(s) 284, 324, 827
MunI CAATTG 1 cut(s) 1155
MvaI CCWGG 2 cut(s) 324, 827
MvnI CGCG 1 cut(s) 868
MwoI GCNNNNNNNGC 3 cut(s) 318, 836, 1325
NciI CCSGG 1 cut(s) 284
NdeII GATC 4 cut(s) 97, 169, 415, 883
NlaIV GGNNCC 6 cut(s) 134, 282, 328, 535, 892, 993
NmuCI GTSAC 5 cut(s) 262, 460, 472, 1150, 1337
NspI RCATGY 2 cut(s) 358, 1183
NspV TTCGAA 1 cut(s) 1222
OliI CACNNNNGTG 1 cut(s) 1298
PaeR7I CTCGAG 1 cut(s) 861
PagI TCATGA 1 cut(s) 418
PaqCI CACCTGC 1 cut(s) 989
PciI ACATGT 2 cut(s) 354, 1179
PdmI GAANNNNTTC 3 cut(s) 77, 179, 573
PfeI GAWTC 4 cut(s) 466, 514, 761, 1173
PflMI CCANNNNNTGG 1 cut(s) 944
PkrI GCNGC 4 cut(s) 899, 910, 1008, 1033
PleI GAGTC 3 cut(s) 73, 805, 1114
PpsI GAGTC 3 cut(s) 73, 805, 1114
PpuMI RGGWCCY 1 cut(s) 280
PscI ACATGT 2 cut(s) 354, 1179
Psp124BI GAGCTC 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 2 cut(s) 322, 825
PspEI GGTNACC 1 cut(s) 262
PspGI CCWGG 2 cut(s) 322, 825
PspN4I GGNNCC 6 cut(s) 134, 282, 328, 535, 892, 993
PspPI GGNCC 4 cut(s) 133, 280, 400, 665
PspPPI RGGWCCY 1 cut(s) 280
PspXI VCTCGAGB 1 cut(s) 861
PsrI GAACNNNNNNTAC 2 cut(s) 953, 985
PstI CTGCAG 1 cut(s) 902
PvuII CAGCTG 1 cut(s) 897
RsaI GTAC 3 cut(s) 117, 1121, 1232
RsaNI GTAC 3 cut(s) 116, 1120, 1231
RseI CAYNNNNRTG 3 cut(s) 696, 1092, 1298
SacI GAGCTC 1 cut(s) 8
SaqAI TTAA 1 cut(s) 1059
SatI GCNGC 4 cut(s) 898, 909, 1007, 1032
Sau3AI GATC 4 cut(s) 97, 169, 415, 883
Sau96I GGNCC 4 cut(s) 133, 280, 400, 665
SchI GAGTC 3 cut(s) 74, 806, 1115
ScrFI CCNGG 3 cut(s) 284, 324, 827
SduI GDGCHC 1 cut(s) 8
SfaNI GCATC 2 cut(s) 501, 1306
SfcI CTRYAG 1 cut(s) 898
Sfr274I CTCGAG 1 cut(s) 861
SfuI TTCGAA 1 cut(s) 1222
SinI GGWCC 3 cut(s) 280, 400, 665
SlaI CTCGAG 1 cut(s) 861
SmiMI CAYNNNNRTG 3 cut(s) 696, 1092, 1298
SmlI CTYRAG 3 cut(s) 206, 638, 861
SmoI CTYRAG 3 cut(s) 206, 638, 861
SsiI CCGC 5 cut(s) 756, 794, 935, 1052, 1211
SspI AATATT 1 cut(s) 165
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 3 cut(s) 282, 322, 825
StyI CCWWGG 1 cut(s) 523
TaaI ACNGT 1 cut(s) 942
TaqI TCGA 3 cut(s) 862, 1145, 1222
TatI WGTACW 1 cut(s) 115
TfiI GAWTC 4 cut(s) 466, 514, 761, 1173
Tru1I TTAA 1 cut(s) 1059
Tru9I TTAA 1 cut(s) 1059
TscAI CASTG 3 cut(s) 524, 945, 1252
TseFI GTSAC 5 cut(s) 262, 460, 472, 1150, 1337
TseI GCWGC 4 cut(s) 897, 908, 1006, 1031
Tsp45I GTSAC 5 cut(s) 262, 460, 472, 1150, 1337
TspDTI ATGAA 7 cut(s) 224, 325, 439, 487, 527, 694, 1281
TspGWI ACGGA 2 cut(s) 491, 1310
TspRI CASTG 3 cut(s) 524, 945, 1252
Van91I CCANNNNNTGG 1 cut(s) 944
VpaK11BI GGWCC 3 cut(s) 280, 400, 665
XagI CCTNNNNNAGG 1 cut(s) 885
XapI RAATTY 5 cut(s) 24, 332, 570, 1193, 1355
XceI RCATGY 2 cut(s) 358, 1183
XcmI CCANNNNNNNNNTGG 2 cut(s) 193, 901
XhoI CTCGAG 1 cut(s) 861
XmnI GAANNNNTTC 3 cut(s) 77, 179, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.