Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002179

Physical Location & Seq

Genomic Coordinates Reverse (-)

6325574 .. 6331974

Gene Structure 6401 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1091200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2034 bp

ATGCCTGAGCTGCGTCCACGCAGAAACCCAGACCCGATCACCAAGACCCAGCCTCGGAGGGCGACTGTACGGACTCGGCGGAACAAGAAGAATAGTAATAATAATCAGAAGCAGAAAAAGAATAATAATAAAAAACAAAAGAAGGCAGCTGGTGCCCGCAAGCGGAGGTGGGCCGAGGGCGGTCCGGTGATTGAAACGAGGTCGTTTAAGGAAGACATAGAGGAGGAGGCAAGTGAGAGAGCAATGGACGAGTACGATAGTGGCGGCCACAGTGGCGATAAGGGCCTTGCAGCTGACGACGAGGGCGCAGCTCCCATTCCCGAGAAGGTCCAGGTTGGTGGTTCCCCAGTGTACAAAATAGAGAAGAAATTGGGGAAAGGAGGCTTTGGTCAAGTATATGTTGGTCGACGGATGGGGGCTGGTCCTGGAGCTGTTGAGGTTGCATTGAAATTTGAGCATAGAAGTAGCAAAGGATGTAATTATGGCCCACCTTATGAATGGCAGATTTACAATGCACTTGGCGGTAGTCATGGGGTGCCAAGAGTTCACTATAAGGGTCGGCAAGCTGACTACTATATCATGGTGATGGATATGCTCGGACCTAGTTTGTGGGATGTCTGGAATAACAGCTCACACACGATGTCAATTGAAATGGTTGCATGTATTGCCATTGAGGCAATATCCATATTGGAGAAGATGCACTCTAGAGGATATGTACATGGGGATGTGAAACCTGAGAACTTTTTGCTTGGCCCCCCTGGAACTGTCGAGGATAAGAAGCTGTTTCTTGTTGACCTTGGATTAGCCACCAGGTGGCGAGATTCGACTGGCCTGCATGTCGACTATGATCAACGTCCAGATGTTTTCAGGGGAACGGTGCGATATGCAAGTGTGCATGCTCATCTTGGTAGAACTGGTAGCAGGAGGGATGACTTGGAATCTCTAGCTTACACACTGATATTTCTTCTTCGTGGTCGGTTGCCATGGCAAGGATATCAGGGAGAAAATAAAGGTTTTCTTGTCTGCAAGAAAAAGATGGCCACTTCCCCAGAAACTTTGTGTTGCTTCTGCCCTCAGCCATTCCGACAATTTGTTGAGTATGTGGTGAATTTGAAGTTTGATGAAGAACCAAACTATGCAAAGTACATATCCCTTTTTGATGGAATTGTTGGCCCAAACCCAGATGTCAGGCCAATAAATACTGATGGTGCTCAGAAGCTTATATGCCAAGTTGGACAAAAGAGAGCAAGATTGACCTTGGAGGAAGAGGATGATGAGCAACCAAAGAAGAAGATTCGTATGGGGATGCCTGCAACCCAATGGATTAGTGTATACAATGCTCGTATGCCTATGAAGCAAAGATATCACTATAATGTTGCAGATGTGAGGCTCTCCCAACACATTGAGAAAGGAAATGAAGATGGGTTATTTATTAGCAGTGTAGCTTCCAGTTCAAATCTTTGGGCTCTTATCATGGATGCTGGCACTGGCTTCAGTGCCCAAGTTTATGAACTCTCTTCATATTTTCTGCATAAGGAATGGATTATGGAGCAGTGGGACAAGAATTACTATATCAGCACGATAGCAGGAGCAAACAATGGAAGCTCTTTAGTTGTAATGTCTAAAGGGACCCAATACTTACAGCAGTCCTATAAAGTCAGCGATTCATTTCCTTTCAAGTGGATTAATAAGAAATGGCGGGAAGGATTTCATGTTACTGCAATGGCAACTGCTGGGAGTAGATGGGCTATTGTTATGTCCCGTGGTGCCGGATTTTCTGACCAGGTTGTGGAACTAGATTTTCTGTATCCAAGCGAAGGCATTCATCGAAGGTGGGATAATGGCTACCGCATCACGTCAACTGCAGCAACTTGGGATCAGGCAGCTTTTGTCCTTAGTGTTCCAAGGAGAAAACTGGCAGATGAAACTCAAGAGACGCTTCGGACTTCTGCTTTTCCTAGTACACACGTTAAGGAGAAATGGGGAAAGAACCTTTATATTGCATCTATTTGCTACGGTAGAACTGTCTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0030100 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0032879 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0060627 GO:0065007 GO:0071704 GO:0097159 GO:0097367 GO:0098657 GO:0140096 GO:1901265 GO:1901363 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

678

Amino Acids

76.6

Weight (kDa)

9.32

Isoelectric Point (pI)

49.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase_fungal	PF17667	80 - 321	1.9e-07	Fungal protein kinase
Pkinase	PF00069	118 - 344	1.8e-17	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	119 - 274	8.2e-07	Protein tyrosine and serine/threonine kinase
DUF7477	PF24289	410 - 677	9.5e-164	Domain of unknown function (DUF7477)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002179)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25760 AT2G25760
fragaria_vesca	FvH4_2g35440 FvH4_2g35440 FvH4_6g32280
malus_domestica	MD08G1111700.v1.1 MD09G1204700.v1.1 MD15G1091200.v1.1 MD17G1184900.v1.1
prunus_persica	Prupe.1G445600_v2.0.a1 Prupe.1G445600_v2.0.a1 Prupe.1G445600_v2.0.a1 Prupe.1G445600_v2.0.a1 Prupe.1G445600_v2.0.a1 Prupe.3G060400_v2.0.a1 Prupe.3G060400_v2.0.a1
pyrus_communis	pycom08g09280 pycom09g12160 pycom15g08520 pycom17g19440
rosa_chinensis	RchiOBHm_Chr2g0140231 RchiOBHm_Chr6g0306451
rosa_laevigata	RLG00000004784 RLG00000008671 RLG00000010797 RLG00000019857
rosa_multiflora	Rmu_sc0002971.1_g000017 Rmu_sc0005446.1_g000008
rosa_roxburghii	Rroxscaffold_2G00104970 Rroxscaffold_7G00161760
rosa_rugosa	Rorug02G0361500 Rorug06G0349500 Rorug06G0349600
rosa_samantha	Rh2AG412400 Rh2BG422700 Rh2CG398600 Rh6AG461700 Rh6BG439300 Rh6CG475800 Rh6DG462500
rosa_wichuraiana	Rw2G033800 Rw6G040240 Rw7G007580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	152, 535, 1766
AccB7I	CCANNNNNTGG	2 cut(s)	813, 1789
AccI	GTMKAC	3 cut(s)	406, 840, 1332
AciI	CCGC	8 cut(s)	79, 157, 163, 180, 264, 522, 1699, 1849
AclWI	GGATC	1 cut(s)	1884
AcoI	YGGCCR	2 cut(s)	265, 1038
AcsI	RAATTY	2 cut(s)	449, 1108
AcuI	CTGAAG	1 cut(s)	1477
AdeI	CACNNNGTG	1 cut(s)	813
AfaI	GTAC	6 cut(s)	69, 254, 353, 717, 1145, 1963
AfiI	CCNNNNNNNGG	6 cut(s)	54, 162, 813, 989, 1789, 1817
AflIII	ACRYGT	1 cut(s)	1966
AgsI	TTSAA	6 cut(s)	194, 448, 650, 1114, 1455, 1678
AjiI	CACGTC	1 cut(s)	1857
AjnI	CCWGG	5 cut(s)	330, 424, 757, 809, 1782
Alw21I	GWGCWC	1 cut(s)	1213
Alw26I	GTCTC	1 cut(s)	1928
AlwI	GGATC	1 cut(s)	1884
Ama87I	CYCGRG	1 cut(s)	320
AoxI	GGCC	9 cut(s)	171, 265, 283, 484, 751, 829, 1038, 1171, 1190
ApeKI	GCWGC	6 cut(s)	10, 146, 290, 308, 1865, 1883
ApoI	RAATTY	2 cut(s)	449, 1108
AseI	ATTAAT	1 cut(s)	1686
Asp700I	GAANNNNTTC	2 cut(s)	1707, 1821
AspLEI	GCGC	1 cut(s)	308
AsuHPI	GGTGA	4 cut(s)	31, 199, 595, 1117
AvaI	CYCGRG	1 cut(s)	320
AvaII	GGWCC	5 cut(s)	182, 328, 422, 599, 1629
BaeGI	GKGCMC	2 cut(s)	157, 1501
BalI	TGGCCA	1 cut(s)	1040
BanI	GGYRCC	3 cut(s)	152, 535, 1766
BanII	GRGCYC	1 cut(s)	1468
BbsI	GAAGAC	1 cut(s)	219
Bbv12I	GWGCWC	1 cut(s)	1213
BbvCI	CCTCAGC	1 cut(s)	1074
BbvI	GCAGC	5 cut(s)	158, 302, 320, 1877, 1895
BccI	CCATC	7 cut(s)	406, 580, 1030, 1154, 1199, 1415, 1737
BcgI	CGANNNNNNTGC	2 cut(s)	814, 848
BciT130I	CCWGG	5 cut(s)	332, 426, 759, 811, 1784
BciVI	GTATCC	1 cut(s)	1818
BclI	TGATCA	1 cut(s)	847
BcoDI	GTCTC	1 cut(s)	1928
BfaI	CTAG	5 cut(s)	603, 705, 944, 1796, 1959
BfmI	CTRYAG	1 cut(s)	1863
BfuI	GTATCC	1 cut(s)	1818
BglI	GCCNNNNNGGC	2 cut(s)	273, 674
BisI	GCNGC	7 cut(s)	11, 147, 265, 291, 309, 1866, 1884
BlsI	GCNGC	7 cut(s)	12, 148, 266, 292, 310, 1867, 1885
Bme1390I	CCNGG	5 cut(s)	332, 426, 759, 811, 1784
Bme18I	GGWCC	5 cut(s)	182, 328, 422, 599, 1629
BmeT110I	CYCGRG	1 cut(s)	320
BmgBI	CACGTC	1 cut(s)	1857
BmiI	GGNNCC	7 cut(s)	154, 343, 537, 754, 1630, 1631, 1768
BmrFI	CCNGG	5 cut(s)	332, 426, 759, 811, 1784
BmrI	ACTGGG	1 cut(s)	341
BmsI	GCATC	5 cut(s)	687, 1296, 1468, 1860, 2012
BmuI	ACTGGG	1 cut(s)	341
BpiI	GAAGAC	1 cut(s)	219
BpmI	CTGGAG	1 cut(s)	447
Bpu10I	CCTNAGC	2 cut(s)	6, 1074
BpuEI	CTTGAG	1 cut(s)	1914
BsaJI	CCNNGG	8 cut(s)	53, 174, 757, 796, 983, 1257, 1762, 1904
BsaWI	WCCGGW	1 cut(s)	184
Bsc4I	CCNNNNNNNGG	6 cut(s)	54, 162, 813, 989, 1789, 1817
Bse1I	ACTGG	6 cut(s)	347, 832, 919, 1449, 1492, 1920
Bse3DI	GCAATG	2 cut(s)	249, 1728
BseBI	CCWGG	5 cut(s)	332, 426, 759, 811, 1784
BseDI	CCNNGG	8 cut(s)	53, 174, 757, 796, 983, 1257, 1762, 1904
BseGI	GGATG	8 cut(s)	417, 479, 619, 730, 934, 1276, 1311, 1483
BseLI	CCNNNNNNNGG	6 cut(s)	54, 162, 813, 989, 1789, 1817
BseMI	GCAATG	2 cut(s)	249, 1728
BseMII	CTCAG	3 cut(s)	726, 1088, 1226
BseNI	ACTGG	6 cut(s)	347, 832, 919, 1449, 1492, 1920
BseRI	GAGGAG	2 cut(s)	236, 239
BseSI	GKGCMC	2 cut(s)	157, 1501
BseXI	GCAGC	5 cut(s)	158, 302, 320, 1877, 1895
BseYI	CCCAGC	2 cut(s)	48, 1733
BshFI	GGCC	9 cut(s)	173, 267, 285, 486, 753, 831, 1040, 1173, 1192
BshNI	GGYRCC	3 cut(s)	152, 535, 1766
BsiHKAI	GWGCWC	1 cut(s)	1213
BsiHKCI	CYCGRG	1 cut(s)	320
BsiSI	CCGG	2 cut(s)	185, 1770
BslFI	GGGAC	3 cut(s)	1571, 1642, 1744
BslI	CCNNNNNNNGG	6 cut(s)	54, 162, 813, 989, 1789, 1817
BsmAI	GTCTC	1 cut(s)	1928
BsmBI	CGTCTC	1 cut(s)	1928
BsmFI	GGGAC	3 cut(s)	1571, 1642, 1744
BsmI	GAATGC	1 cut(s)	1821
BsnI	GGCC	9 cut(s)	173, 267, 285, 486, 753, 831, 1040, 1173, 1192
BsoBI	CYCGRG	1 cut(s)	320
Bsp1286I	GDGCHC	4 cut(s)	157, 1213, 1468, 1501
Bsp1407I	TGTACA	2 cut(s)	351, 715
Bsp143I	GATC	3 cut(s)	36, 847, 1876
Bsp19I	CCATGG	1 cut(s)	983
BspACI	CCGC	8 cut(s)	79, 157, 163, 180, 264, 522, 1699, 1849
BspANI	GGCC	9 cut(s)	173, 267, 285, 486, 753, 831, 1040, 1173, 1192
BspCNI	CTCAG	3 cut(s)	727, 1087, 1225
BspHI	TCATGA	1 cut(s)	2030
BspLI	GGNNCC	7 cut(s)	154, 343, 537, 754, 1630, 1631, 1768
BspMAI	CTGCAG	1 cut(s)	1867
BspPI	GGATC	1 cut(s)	1884
BspT107I	GGYRCC	3 cut(s)	152, 535, 1766
BsrDI	GCAATG	2 cut(s)	249, 1728
BsrGI	TGTACA	2 cut(s)	351, 715
BsrI	ACTGG	6 cut(s)	347, 832, 919, 1449, 1492, 1920
BssECI	CCNNGG	8 cut(s)	53, 174, 757, 796, 983, 1257, 1762, 1904
BssMI	GATC	3 cut(s)	36, 847, 1876
BssNAI	GTATAC	1 cut(s)	1333
BssT1I	CCWWGG	4 cut(s)	796, 983, 1257, 1904
Bst1107I	GTATAC	1 cut(s)	1333
Bst2UI	CCWGG	5 cut(s)	332, 426, 759, 811, 1784
Bst4CI	ACNGT	6 cut(s)	67, 272, 766, 877, 2018, 2026
Bst6I	CTCTTC	2 cut(s)	1260, 1522
BstAPI	GCANNNNNTGC	2 cut(s)	152, 665
BstAUI	TGTACA	2 cut(s)	351, 715
BstC8I	GCNNGC	7 cut(s)	157, 161, 564, 833, 897, 1311, 1483
BstDEI	CTNAG	5 cut(s)	6, 735, 1074, 1212, 1895
BstDSI	CCRYGG	2 cut(s)	983, 1762
BstF5I	GGATG	8 cut(s)	417, 479, 619, 730, 934, 1276, 1311, 1483
BstHHI	GCGC	1 cut(s)	308
BstKTI	GATC	3 cut(s)	39, 850, 1879
BstMAI	GTCTC	1 cut(s)	1928
BstMBI	GATC	3 cut(s)	36, 847, 1876
BstMWI	GCNNNNNNNGC	7 cut(s)	10, 152, 273, 282, 665, 674, 1354
BstNI	CCWGG	5 cut(s)	332, 426, 759, 811, 1784
BstNSI	RCATGY	3 cut(s)	663, 839, 899
BstSCI	CCNGG	5 cut(s)	330, 424, 757, 809, 1782
BstSFI	CTRYAG	1 cut(s)	1863
BstSLI	GKGCMC	2 cut(s)	157, 1501
BstV1I	GCAGC	5 cut(s)	158, 302, 320, 1877, 1895
BstV2I	GAAGAC	1 cut(s)	219
BstXI	CCANNNNNNTGG	1 cut(s)	338
BstZ17I	GTATAC	1 cut(s)	1333
BsuI	GTATCC	1 cut(s)	1818
BsuRI	GGCC	9 cut(s)	173, 267, 285, 486, 753, 831, 1040, 1173, 1192
BtgI	CCRYGG	2 cut(s)	983, 1762
BtrI	CACGTC	1 cut(s)	1857
BtsCI	GGATG	8 cut(s)	417, 479, 619, 730, 934, 1276, 1311, 1483
BtsI	GCAGTG	2 cut(s)	1444, 1559
BtsIMutI	CAGTG	7 cut(s)	277, 354, 953, 1444, 1485, 1501, 1559
Cac8I	GCNNGC	7 cut(s)	157, 161, 564, 833, 897, 1311, 1483
CciI	TCATGA	1 cut(s)	2030
CfoI	GCGC	1 cut(s)	308
CpoI	CGGWCCG	1 cut(s)	182
CseI	GACGC	2 cut(s)	2, 1945
CsiI	ACCWGGT	2 cut(s)	809, 1782
Csp6I	GTAC	6 cut(s)	68, 253, 352, 716, 1144, 1962
CspI	CGGWCCG	1 cut(s)	182
CviQI	GTAC	6 cut(s)	68, 253, 352, 716, 1144, 1962
DdeI	CTNAG	5 cut(s)	6, 735, 1074, 1212, 1895
DpnI	GATC	3 cut(s)	38, 849, 1878
DpnII	GATC	3 cut(s)	36, 847, 1876
DraIII	CACNNNGTG	1 cut(s)	813
EaeI	YGGCCR	2 cut(s)	265, 1038
Eam1104I	CTCTTC	2 cut(s)	1260, 1522
EarI	CTCTTC	2 cut(s)	1260, 1522
EciI	GGCGGA	1 cut(s)	94
Eco130I	CCWWGG	4 cut(s)	796, 983, 1257, 1904
Eco24I	GRGCYC	1 cut(s)	1468
Eco32I	GATATC	2 cut(s)	995, 1364
Eco47I	GGWCC	5 cut(s)	182, 328, 422, 599, 1629
Eco57I	CTGAAG	1 cut(s)	1477
Eco88I	CYCGRG	1 cut(s)	320
EcoO109I	RGGNCCY	2 cut(s)	283, 1629
EcoRII	CCWGG	5 cut(s)	330, 424, 757, 809, 1782
EcoRV	GATATC	2 cut(s)	995, 1364
EcoT14I	CCWWGG	4 cut(s)	796, 983, 1257, 1904
EcoT38I	GRGCYC	1 cut(s)	1468
ErhI	CCWWGG	4 cut(s)	796, 983, 1257, 1904
Esp3I	CGTCTC	1 cut(s)	1928
FalI	AAGNNNNNCTT	4 cut(s)	1002, 1034, 1923, 1955
FaqI	GGGAC	3 cut(s)	1571, 1642, 1744
FauI	CCCGC	2 cut(s)	164, 1692
FbaI	TGATCA	1 cut(s)	847
FblI	GTMKAC	3 cut(s)	406, 840, 1332
Fnu4HI	GCNGC	7 cut(s)	11, 147, 265, 291, 309, 1866, 1884
FokI	GGATG	8 cut(s)	424, 486, 626, 737, 941, 1283, 1318, 1490
FriOI	GRGCYC	1 cut(s)	1468
Fsp4HI	GCNGC	7 cut(s)	11, 147, 265, 291, 309, 1866, 1884
FspBI	CTAG	5 cut(s)	603, 705, 944, 1796, 1959
GlaI	GCGC	1 cut(s)	307
GluI	GCNGC	7 cut(s)	11, 147, 265, 291, 309, 1866, 1884
GsaI	CCCAGC	2 cut(s)	52, 1737
GsuI	CTGGAG	1 cut(s)	447
HaeIII	GGCC	9 cut(s)	173, 267, 285, 486, 753, 831, 1040, 1173, 1192
HapII	CCGG	2 cut(s)	185, 1770
HgaI	GACGC	2 cut(s)	2, 1945
HhaI	GCGC	1 cut(s)	308
Hin6I	GCGC	1 cut(s)	306
HinP1I	GCGC	1 cut(s)	306
HincII	GTYRAC	4 cut(s)	407, 793, 841, 1860
HindII	GTYRAC	4 cut(s)	407, 793, 841, 1860
HindIII	AAGCTT	1 cut(s)	1217
HinfI	GANTC	5 cut(s)	73, 821, 938, 1294, 1664
HpaII	CCGG	2 cut(s)	185, 1770
HphI	GGTGA	4 cut(s)	31, 199, 595, 1117
Hpy166II	GTNNAC	9 cut(s)	17, 352, 407, 547, 793, 841, 1333, 1860, 1964
Hpy188I	TCNGA	7 cut(s)	57, 108, 599, 1085, 1215, 1780, 1944
Hpy188III	TCNNGA	6 cut(s)	320, 619, 705, 857, 1931, 2031
Hpy8I	GTNNAC	9 cut(s)	17, 352, 407, 547, 793, 841, 1333, 1860, 1964
Hpy99I	CGWCG	2 cut(s)	302, 411
HpyAV	CCTTC	5 cut(s)	136, 319, 1697, 1811, 1824
HpyCH4III	ACNGT	6 cut(s)	67, 272, 766, 877, 2018, 2026
HpyCH4IV	ACGT	3 cut(s)	853, 1856, 1968
HpyF10VI	GCNNNNNNNGC	7 cut(s)	10, 152, 273, 282, 665, 674, 1354
HpyF3I	CTNAG	5 cut(s)	6, 735, 1074, 1212, 1895
HpySE526I	ACGT	3 cut(s)	853, 1856, 1968
HspAI	GCGC	1 cut(s)	306
KflI	GGGWCCC	1 cut(s)	1629
Ksp22I	TGATCA	1 cut(s)	847
Kzo9I	GATC	3 cut(s)	36, 847, 1876
LmnI	GCTCC	4 cut(s)	316, 428, 1549, 1589
Lsp1109I	GCAGC	5 cut(s)	158, 302, 320, 1877, 1895
LweI	GCATC	5 cut(s)	687, 1296, 1468, 1860, 2012
MabI	ACCWGGT	2 cut(s)	809, 1782
MaeI	CTAG	5 cut(s)	603, 705, 944, 1796, 1959
MaeII	ACGT	3 cut(s)	853, 1856, 1968
MaeIII	GTNAC	1 cut(s)	1714
MalI	GATC	3 cut(s)	38, 849, 1878
MboI	GATC	3 cut(s)	36, 847, 1876
MfeI	CAATTG	1 cut(s)	645
MhlI	GDGCHC	4 cut(s)	157, 1213, 1468, 1501
MlsI	TGGCCA	1 cut(s)	1040
MluCI	AATT	8 cut(s)	368, 449, 478, 645, 1088, 1108, 1164, 1564
MluNI	TGGCCA	1 cut(s)	1040
MlyI	GAGTC	1 cut(s)	67
MmeI	TCCRAC	2 cut(s)	1108, 1213
Mox20I	TGGCCA	1 cut(s)	1040
MroXI	GAANNNNTTC	2 cut(s)	1707, 1821
MscI	TGGCCA	1 cut(s)	1040
MseI	TTAA	3 cut(s)	207, 1686, 1971
MslI	CAYNNNNRTG	3 cut(s)	584, 723, 1371
Msp20I	TGGCCA	1 cut(s)	1040
MspA1I	CMGCKG	2 cut(s)	149, 293
MspI	CCGG	2 cut(s)	185, 1770
MspR9I	CCNGG	5 cut(s)	332, 426, 759, 811, 1784
MunI	CAATTG	1 cut(s)	645
Mva1269I	GAATGC	1 cut(s)	1821
MvaI	CCWGG	5 cut(s)	332, 426, 759, 811, 1784
MwoI	GCNNNNNNNGC	7 cut(s)	10, 152, 273, 282, 665, 674, 1354
NcoI	CCATGG	1 cut(s)	983
NdeII	GATC	3 cut(s)	36, 847, 1876
NlaIV	GGNNCC	7 cut(s)	154, 343, 537, 754, 1630, 1631, 1768
NmeAIII	GCCGAG	2 cut(s)	55, 199
NspI	RCATGY	3 cut(s)	663, 839, 899
PaeI	GCATGC	1 cut(s)	899
PagI	TCATGA	1 cut(s)	2030
PctI	GAATGC	1 cut(s)	1821
PdmI	GAANNNNTTC	2 cut(s)	1707, 1821
PfeI	GAWTC	4 cut(s)	821, 938, 1294, 1664
PflMI	CCANNNNNTGG	2 cut(s)	813, 1789
PfoI	TCCNGGA	1 cut(s)	424
PkrI	GCNGC	7 cut(s)	12, 148, 266, 292, 310, 1867, 1885
PleI	GAGTC	1 cut(s)	67
PpsI	GAGTC	1 cut(s)	67
PpuMI	RGGWCCY	1 cut(s)	1629
PshBI	ATTAAT	1 cut(s)	1686
Psp5II	RGGWCCY	1 cut(s)	1629
Psp6I	CCWGG	5 cut(s)	330, 424, 757, 809, 1782
PspFI	CCCAGC	2 cut(s)	48, 1733
PspGI	CCWGG	5 cut(s)	330, 424, 757, 809, 1782
PspN4I	GGNNCC	7 cut(s)	154, 343, 537, 754, 1630, 1631, 1768
PspPPI	RGGWCCY	1 cut(s)	1629
PstI	CTGCAG	1 cut(s)	1867
PvuII	CAGCTG	2 cut(s)	149, 293
RsaI	GTAC	6 cut(s)	69, 254, 353, 717, 1145, 1963
RsaNI	GTAC	6 cut(s)	68, 253, 352, 716, 1144, 1962
RseI	CAYNNNNRTG	3 cut(s)	584, 723, 1371
Rsr2I	CGGWCCG	1 cut(s)	182
RsrII	CGGWCCG	1 cut(s)	182
SalI	GTCGAC	2 cut(s)	405, 839
SaqAI	TTAA	3 cut(s)	207, 1686, 1971
SatI	GCNGC	7 cut(s)	11, 147, 265, 291, 309, 1866, 1884
Sau3AI	GATC	3 cut(s)	36, 847, 1876
SchI	GAGTC	1 cut(s)	67
ScrFI	CCNGG	5 cut(s)	332, 426, 759, 811, 1784
SduI	GDGCHC	4 cut(s)	157, 1213, 1468, 1501
SexAI	ACCWGGT	2 cut(s)	809, 1782
SfaNI	GCATC	5 cut(s)	687, 1296, 1468, 1860, 2012
SfcI	CTRYAG	1 cut(s)	1863
SinI	GGWCC	5 cut(s)	182, 328, 422, 599, 1629
SmiMI	CAYNNNNRTG	3 cut(s)	584, 723, 1371
SmlI	CTYRAG	1 cut(s)	1929
SmoI	CTYRAG	1 cut(s)	1929
SphI	GCATGC	1 cut(s)	899
Sse9I	AATT	8 cut(s)	368, 449, 478, 645, 1088, 1108, 1164, 1564
SsiI	CCGC	8 cut(s)	79, 157, 163, 180, 264, 522, 1699, 1849
SspMI	CTAG	5 cut(s)	603, 705, 944, 1796, 1959
StyD4I	CCNGG	5 cut(s)	330, 424, 757, 809, 1782
StyI	CCWWGG	4 cut(s)	796, 983, 1257, 1904
TaaI	ACNGT	6 cut(s)	67, 272, 766, 877, 2018, 2026
TaiI	ACGT	3 cut(s)	856, 1859, 1971
TaqI	TCGA	5 cut(s)	406, 768, 824, 840, 1828
TasI	AATT	8 cut(s)	368, 449, 478, 645, 1088, 1108, 1164, 1564
TatI	WGTACW	4 cut(s)	351, 715, 1143, 1961
TauI	GCSGC	1 cut(s)	267
TfiI	GAWTC	4 cut(s)	821, 938, 1294, 1664
Tru1I	TTAA	3 cut(s)	207, 1686, 1971
Tru9I	TTAA	3 cut(s)	207, 1686, 1971
TscAI	CASTG	7 cut(s)	277, 354, 960, 1444, 1492, 1501, 1559
TseI	GCWGC	6 cut(s)	10, 146, 290, 308, 1865, 1883
TspGWI	ACGGA	2 cut(s)	85, 424
TspRI	CASTG	7 cut(s)	277, 354, 960, 1444, 1492, 1501, 1559
Van91I	CCANNNNNTGG	2 cut(s)	813, 1789
VpaK11BI	GGWCC	5 cut(s)	182, 328, 422, 599, 1629
VspI	ATTAAT	1 cut(s)	1686
XapI	RAATTY	2 cut(s)	449, 1108
XbaI	TCTAGA	1 cut(s)	704
XceI	RCATGY	3 cut(s)	663, 839, 899
XcmI	CCANNNNNNNNNTGG	1 cut(s)	495
XmiI	GTMKAC	3 cut(s)	406, 840, 1332
XmnI	GAANNNNTTC	2 cut(s)	1707, 1821
XspI	CTAG	5 cut(s)	603, 705, 944, 1796, 1959

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.