Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002179

Physical Location & Seq

Genomic Coordinates Forward (+)

57531674 .. 57536876

Gene Structure 5203 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000019857

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2097 bp

ATGCCGGTACTGCGTGGAAGGGGCCGTGGAGCAGCTAAAAAGCAGGAGGTTACGAATCCGATCGACGCCGGAGAGGCCATTGCCACCAGAACAAGGCGAAGGCGAGCGGAGGCGGCGGCGGCCGCACTGCCGAATAATAATAATAATAACAACAATAATAATCAGGTGAAGGAGGAAAAGAATCGGAAGGCGGCGGCTGCTGTGGCGGCCGGTGAGGAGAAGAAGGTAGTCGTTAAGGCGGAGAAAAAGAGGGTTGTTGAGAAGGAGGAAGTGAAGGAGAAGAAGATGAATGACAGTGGTAGTGGCGGCCTTAGGAGTAATGCCAAGGCTGAAGAAGAAGGCAGCACTGCTCCTCTTCCTGAGAAGGTTACTGTTGGTGGTTCCCCTGTCTACAGATTAGATAGAAAGCTGGGTAAGGGTGGCTTTGGACAAGTGTATGTCGGTCGGCGTGTTTCTGCTATAAATACGAATGAGAGAACTGGACCAGGAGCTGTTGAGGTGGCCTTAAAACTCGAACACAAAAATAGTAAAGGGTGCAATTATGGACCGCCACAGGAGTGGAAAGTTTATGACACTCTTGGTGGCAGCCATGGTGTGCCGCGGGTGCACTATAAGGGTAAGCAAGGCGAGTATTATATTATGGTGATGGACATGTTGGGGCCAAGCTTGTGGGATGTTTGGAATAATGGCAATCAAACAATGGCCATTGAAATGGTTGCGTGTATTGCCATTGAAGCAATATCAATATTGGAGAAGATGCATTCTCGAGGGTATGTACATGGAGATGTAAAGCCTGAAAACTTTCTACTTGGTACTCCTGGGACACCTGATGAGAAAAAGCTATTTTTAGTTGATCTTGGACTAGCAACTAAATGGCGAGATAGTTCAACCGGTCAGCATGTTGATTATGACCAAAGGCCCGATGTTTTCAGAGGAACAGTACGTTACGCTAGTGTGCATGCTCATTTAGGGAGAACGAGTAGTAGGAGAGATGATCTTGAGTCTCTAGCTTATACCCTTGTATTCCTTCTCCGTGGTCGCCTCCCCTGGCAAGGATACCAGGGAGAGAATAAAGGATTCCTTGTCTGCAAGAAGAAGATGGCAACGTCTCCAGAGACCTTGTGTTCTTTCTGTCCACTCCCTTTTAGACAATTTGTTGAACATGTGGTGAACTTGAAGTTTGATGAAGAACCTAATTATGCAAAATATATATCATTATTTGATGGGACTGTAAGTCCAAATCCTGATATCAGGCCAATAAACACAGAGGGTGCACAGAAGCTTATTAATTTGGTTGGTCATAAGAGAGGAAGATTAACAATGGAGGAGGAGGATGATGAACAACCAAAGAAGAGAATTAGAATGGGTATGCCAGCAACACAATGGATCAGTGTTTATAATGCTCGGAGACCGATGAAGCAAAGGTATCACTATAATGTGGCTGATACAAGGCTTGCTCAGCATATTGAGAAAGGGAATGAGGATGGGTTATATATTAGCAGTGTGGCTTCTTCTCAAAATTTGTGGGCCTTGATTATGGATGCAGGCACTGGTTTCACTTCCCAAGTTTATGAACTTGCGCCGACTTGTCTTCACAAGGAATGGATAATGGAGCAGTGGGAGAAAAACTATTACATCAGTGCATTAGCCGGAGCTACTAATGGGAGTTCATTAGTAGTAATGTCCAAGGGTACAACTTTTACGCAGCAGTCATACAAAGTCAGTGATACATTTCCATTTAAGTGGATTAACAAAAAGTGGAAAGAGGACTTTTTTGTTACGTCAATGGCCACTGCAGGAAGTAGATGGGCCATTGTTATGTCTCGGGGTGCTAATTATTCAGACCAAGTTGTTGAACTAGATTTCCTTTATCCTAGTGAAGGAATACATCGGCGCTGGGATTCTGGATATCGTATAACTGCAACTGCAGCAACATGGGATCAGGCCGCTTTTGTTCTCAGCGTGCCAAGGAAGAAGCCTCAAGATGAAACCCAGGAGACGCTTCGTACTTCTGCTTTTCCAAGTACACATGTCAAGGAGAAGTGGGCGAAGAACCTTTACATTGCATCTATTTGTTATGGACGAACAGTATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0030100 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0032879 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0060627 GO:0065007 GO:0071704 GO:0097159 GO:0097367 GO:0098657 GO:0140096 GO:1901265 GO:1901363 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

699

Amino Acids

78.26

Weight (kDa)

9.33

Isoelectric Point (pI)

44.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	131 - 365	3.7e-16	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	133 - 297	4e-07	Protein tyrosine and serine/threonine kinase
Pkinase_fungal	PF17667	179 - 342	1.2e-08	Fungal protein kinase
DUF7477	PF24289	432 - 698	4e-162	Domain of unknown function (DUF7477)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002179)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25760 AT2G25760
fragaria_vesca	FvH4_2g35440 FvH4_2g35440 FvH4_6g32280
malus_domestica	MD08G1111700.v1.1 MD09G1204700.v1.1 MD15G1091200.v1.1 MD17G1184900.v1.1
prunus_persica	Prupe.1G445600_v2.0.a1 Prupe.1G445600_v2.0.a1 Prupe.1G445600_v2.0.a1 Prupe.1G445600_v2.0.a1 Prupe.1G445600_v2.0.a1 Prupe.3G060400_v2.0.a1 Prupe.3G060400_v2.0.a1
pyrus_communis	pycom08g09280 pycom09g12160 pycom15g08520 pycom17g19440
rosa_chinensis	RchiOBHm_Chr2g0140231 RchiOBHm_Chr6g0306451
rosa_laevigata	RLG00000004784 RLG00000008671 RLG00000010797 RLG00000019857
rosa_multiflora	Rmu_sc0002971.1_g000017 Rmu_sc0005446.1_g000008
rosa_roxburghii	Rroxscaffold_2G00104970 Rroxscaffold_7G00161760
rosa_rugosa	Rorug02G0361500 Rorug06G0349500 Rorug06G0349600
rosa_samantha	Rh2AG412400 Rh2BG422700 Rh2CG398600 Rh6AG461700 Rh6BG439300 Rh6CG475800 Rh6DG462500
rosa_wichuraiana	Rw2G033800 Rw6G040240 Rw7G007580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1398
AccBSI	CCGCTC	1 cut(s)	107
AccI	GTMKAC	1 cut(s)	390
AccII	CGCG	1 cut(s)	601
AclWI	GGATC	2 cut(s)	1394, 1947
AcoI	YGGCCR	4 cut(s)	120, 207, 702, 1788
AcsI	RAATTY	1 cut(s)	1519
AcuI	CTGAAG	1 cut(s)	351
AcyI	GRCGYC	1 cut(s)	66
AfaI	GTAC	7 cut(s)	9, 777, 814, 942, 1693, 2008, 2026
AfiI	CCNNNNNNNGG	3 cut(s)	93, 1052, 1880
AflIII	ACRYGT	3 cut(s)	651, 1162, 2029
AgeI	ACCGGT	1 cut(s)	890
AgsI	TTSAA	6 cut(s)	710, 734, 888, 1160, 1177, 1856
AhdI	GACNNNNNGTC	1 cut(s)	1233
AjnI	CCWGG	5 cut(s)	484, 817, 1046, 1059, 1992
AleI	CACNNNNGTG	1 cut(s)	556
AloI	GAACNNNNNNTCC	2 cut(s)	1848, 1880
AluBI	AGCT	8 cut(s)	35, 409, 491, 666, 841, 1010, 1282, 1655
AluI	AGCT	8 cut(s)	35, 409, 491, 666, 841, 1010, 1282, 1655
Alw21I	GWGCWC	2 cut(s)	609, 1276
Alw26I	GTCTC	6 cut(s)	1008, 1109, 1113, 1402, 1827, 1991
Alw44I	GTGCAC	2 cut(s)	605, 1272
AlwI	GGATC	2 cut(s)	1394, 1947
AlwNI	CAGNNNCTG	2 cut(s)	491, 1550
Ama87I	CYCGRG	2 cut(s)	765, 1824
ApaLI	GTGCAC	2 cut(s)	605, 1272
ApeKI	GCWGC	6 cut(s)	32, 197, 342, 585, 1705, 1928
ApoI	RAATTY	1 cut(s)	1519
AseI	ATTAAT	1 cut(s)	1287
AsiGI	ACCGGT	1 cut(s)	890
Asp700I	GAANNNNTTC	1 cut(s)	801
AspLEI	GCGC	2 cut(s)	1582, 1896
AspS9I	GGNCC	7 cut(s)	22, 482, 545, 659, 918, 1527, 1809
AsuHPI	GGTGA	4 cut(s)	178, 224, 655, 1180
AvaI	CYCGRG	2 cut(s)	765, 1824
AvaII	GGWCC	2 cut(s)	482, 545
AxyI	CCTNAGG	1 cut(s)	311
BaeGI	GKGCMC	2 cut(s)	609, 1276
BalI	TGGCCA	2 cut(s)	704, 1790
BarI	GAAGNNNNNNTAC	2 cut(s)	2042, 2074
BbsI	GAAGAC	1 cut(s)	1583
Bbv12I	GWGCWC	2 cut(s)	609, 1276
BbvI	GCAGC	6 cut(s)	44, 184, 354, 597, 1717, 1940
BccI	CCATC	5 cut(s)	640, 1093, 1217, 1478, 1800
BceAI	ACGGC	1 cut(s)	9
BciT130I	CCWGG	5 cut(s)	486, 819, 1048, 1061, 1994
BciVI	GTATCC	1 cut(s)	1049
BcoDI	GTCTC	6 cut(s)	1008, 1109, 1113, 1402, 1827, 1991
BfaI	CTAG	5 cut(s)	863, 951, 1007, 1859, 1875
BfmI	CTRYAG	3 cut(s)	391, 1794, 1926
BfoI	RGCGCY	1 cut(s)	1897
BfuI	GTATCC	1 cut(s)	1049
BglI	GCCNNNNNGGC	1 cut(s)	74
BlpI	GCTNAGC	1 cut(s)	1458
Bme1390I	CCNGG	5 cut(s)	486, 819, 1048, 1061, 1994
Bme18I	GGWCC	2 cut(s)	482, 545
BmeRI	GACNNNNNGTC	1 cut(s)	1233
BmeT110I	CYCGRG	2 cut(s)	765, 1824
BmgT120I	GGNCC	7 cut(s)	22, 482, 545, 659, 918, 1527, 1809
BmiI	GGNNCC	3 cut(s)	23, 382, 660
BmrFI	CCNGG	5 cut(s)	486, 819, 1048, 1061, 1994
BmsI	GCATC	3 cut(s)	747, 1531, 2075
BpiI	GAAGAC	1 cut(s)	1583
BpmI	CTGGAG	1 cut(s)	1095
Bpu1102I	GCTNAGC	1 cut(s)	1458
BpuEI	CTTGAG	2 cut(s)	1019, 1965
BsaHI	GRCGYC	1 cut(s)	66
BsaI	GGTCTC	2 cut(s)	1109, 1402
BsaWI	WCCGGW	1 cut(s)	890
BsaXI	ACNNNNNCTCC	2 cut(s)	964, 994
Bsc4I	CCNNNNNNNGG	3 cut(s)	93, 1052, 1880
Bse118I	RCCGGY	3 cut(s)	4, 209, 890
Bse1I	ACTGG	2 cut(s)	484, 1555
Bse21I	CCTNAGG	1 cut(s)	311
Bse3DI	GCAATG	2 cut(s)	78, 2061
BseBI	CCWGG	5 cut(s)	486, 819, 1048, 1061, 1994
BseGI	GGATG	4 cut(s)	679, 1339, 1489, 1546
BseLI	CCNNNNNNNGG	3 cut(s)	93, 1052, 1880
BseMI	GCAATG	2 cut(s)	78, 2061
BseMII	CTCAG	3 cut(s)	351, 1472, 1972
BseNI	ACTGG	2 cut(s)	484, 1555
BseRI	GAGGAG	4 cut(s)	230, 342, 1340, 1343
BseSI	GKGCMC	2 cut(s)	609, 1276
BseX3I	CGGCCG	2 cut(s)	120, 207
BseXI	GCAGC	6 cut(s)	44, 184, 354, 597, 1717, 1940
BseYI	CCCAGC	2 cut(s)	409, 1896
Bsh1236I	CGCG	1 cut(s)	601
Bsh1285I	CGRYCG	4 cut(s)	63, 123, 210, 445
BshTI	ACCGGT	1 cut(s)	890
BsiEI	CGRYCG	4 cut(s)	63, 123, 210, 445
BsiHKAI	GWGCWC	2 cut(s)	609, 1276
BsiHKCI	CYCGRG	2 cut(s)	765, 1824
BsiSI	CCGG	5 cut(s)	5, 69, 210, 891, 1650
BslFI	GGGAC	2 cut(s)	835, 1240
BslI	CCNNNNNNNGG	3 cut(s)	93, 1052, 1880
BsmAI	GTCTC	6 cut(s)	1008, 1109, 1113, 1402, 1827, 1991
BsmBI	CGTCTC	2 cut(s)	1113, 1991
BsmFI	GGGAC	2 cut(s)	835, 1240
BsmI	GAATGC	1 cut(s)	760
Bso31I	GGTCTC	2 cut(s)	1109, 1402
BsoBI	CYCGRG	2 cut(s)	765, 1824
Bsp1286I	GDGCHC	2 cut(s)	609, 1276
Bsp1407I	TGTACA	1 cut(s)	775
Bsp143I	GATC	5 cut(s)	60, 853, 994, 1386, 1939
Bsp1720I	GCTNAGC	1 cut(s)	1458
Bsp19I	CCATGG	1 cut(s)	589
BspCNI	CTCAG	3 cut(s)	352, 1471, 1971
BspFNI	CGCG	1 cut(s)	601
BspLI	GGNNCC	3 cut(s)	23, 382, 660
BspMAI	CTGCAG	2 cut(s)	1798, 1930
BspPI	GGATC	2 cut(s)	1394, 1947
BspTNI	GGTCTC	2 cut(s)	1109, 1402
BsrBI	CCGCTC	1 cut(s)	107
BsrDI	GCAATG	2 cut(s)	78, 2061
BsrFI	RCCGGY	3 cut(s)	4, 209, 890
BsrGI	TGTACA	1 cut(s)	775
BsrI	ACTGG	2 cut(s)	484, 1555
BssAI	RCCGGY	3 cut(s)	4, 209, 890
BssMI	GATC	5 cut(s)	60, 853, 994, 1386, 1939
BssNI	GRCGYC	1 cut(s)	66
BssT1I	CCWWGG	4 cut(s)	324, 589, 1686, 1967
Bst2UI	CCWGG	5 cut(s)	486, 819, 1048, 1061, 1994
Bst4CI	ACNGT	5 cut(s)	296, 373, 940, 1231, 2089
Bst6I	CTCTTC	2 cut(s)	360, 1346
BstACI	GRCGYC	1 cut(s)	66
BstAUI	TGTACA	1 cut(s)	775
BstC8I	GCNNGC	6 cut(s)	105, 960, 1374, 1455, 1546, 1964
BstDEI	CTNAG	4 cut(s)	311, 360, 1458, 1958
BstDSI	CCRYGG	4 cut(s)	25, 589, 599, 1033
BstENI	CCTNNNNNAGG	1 cut(s)	1878
BstF5I	GGATG	4 cut(s)	679, 1339, 1489, 1546
BstFNI	CGCG	1 cut(s)	601
BstH2I	RGCGCY	1 cut(s)	1897
BstHHI	GCGC	2 cut(s)	1582, 1896
BstKTI	GATC	5 cut(s)	63, 856, 997, 1389, 1942
BstMAI	GTCTC	6 cut(s)	1008, 1109, 1113, 1402, 1827, 1991
BstMBI	GATC	5 cut(s)	60, 853, 994, 1386, 1939
BstMCI	CGRYCG	4 cut(s)	63, 123, 210, 445
BstNI	CCWGG	5 cut(s)	486, 819, 1048, 1061, 1994
BstNSI	RCATGY	5 cut(s)	655, 902, 962, 1166, 2033
BstSCI	CCNGG	5 cut(s)	484, 817, 1046, 1059, 1992
BstSFI	CTRYAG	3 cut(s)	391, 1794, 1926
BstSLI	GKGCMC	2 cut(s)	609, 1276
BstUI	CGCG	1 cut(s)	601
BstV1I	GCAGC	6 cut(s)	44, 184, 354, 597, 1717, 1940
BstV2I	GAAGAC	1 cut(s)	1583
BstXI	CCANNNNNNTGG	4 cut(s)	558, 669, 712, 1743
BstZI	CGGCCG	2 cut(s)	120, 207
Bsu36I	CCTNAGG	1 cut(s)	311
BsuI	GTATCC	1 cut(s)	1049
BtgI	CCRYGG	4 cut(s)	25, 589, 599, 1033
BtsCI	GGATG	4 cut(s)	679, 1339, 1489, 1546
BtsI	GCAGTG	5 cut(s)	125, 345, 1507, 1622, 1791
Cac8I	GCNNGC	6 cut(s)	105, 960, 1374, 1455, 1546, 1964
CaiI	CAGNNNCTG	2 cut(s)	491, 1550
CciNI	GCGGCCGC	1 cut(s)	120
CfoI	GCGC	2 cut(s)	1582, 1896
Cfr10I	RCCGGY	3 cut(s)	4, 209, 890
Cfr13I	GGNCC	7 cut(s)	22, 482, 545, 659, 918, 1527, 1809
Cfr42I	CCGCGG	1 cut(s)	602
CseI	GACGC	2 cut(s)	74, 2008
Csp6I	GTAC	7 cut(s)	8, 776, 813, 941, 1692, 2007, 2025
CspAI	ACCGGT	1 cut(s)	890
CspCI	CAANNNNNGTGG	2 cut(s)	1505, 1540
CviAII	CATG	8 cut(s)	590, 652, 779, 899, 959, 1163, 1935, 2030
CviQI	GTAC	7 cut(s)	8, 776, 813, 941, 1692, 2007, 2025
DdeI	CTNAG	4 cut(s)	311, 360, 1458, 1958
DpnI	GATC	5 cut(s)	62, 855, 996, 1388, 1941
DpnII	GATC	5 cut(s)	60, 853, 994, 1386, 1939
DriI	GACNNNNNGTC	1 cut(s)	1233
EaeI	YGGCCR	4 cut(s)	120, 207, 702, 1788
EagI	CGGCCG	2 cut(s)	120, 207
Eam1104I	CTCTTC	2 cut(s)	360, 1346
Eam1105I	GACNNNNNGTC	1 cut(s)	1233
EarI	CTCTTC	2 cut(s)	360, 1346
EciI	GGCGGA	1 cut(s)	254
EclXI	CGGCCG	2 cut(s)	120, 207
Eco130I	CCWWGG	4 cut(s)	324, 589, 1686, 1967
Eco31I	GGTCTC	2 cut(s)	1109, 1402
Eco32I	GATATC	2 cut(s)	1249, 1910
Eco47I	GGWCC	2 cut(s)	482, 545
Eco52I	CGGCCG	2 cut(s)	120, 207
Eco57I	CTGAAG	1 cut(s)	351
Eco81I	CCTNAGG	1 cut(s)	311
Eco88I	CYCGRG	2 cut(s)	765, 1824
EcoNI	CCTNNNNNAGG	1 cut(s)	1878
EcoRII	CCWGG	5 cut(s)	484, 817, 1046, 1059, 1992
EcoRV	GATATC	2 cut(s)	1249, 1910
EcoT14I	CCWWGG	4 cut(s)	324, 589, 1686, 1967
EcoT22I	ATGCAT	1 cut(s)	762
ErhI	CCWWGG	4 cut(s)	324, 589, 1686, 1967
Esp3I	CGTCTC	2 cut(s)	1113, 1991
FaeI	CATG	8 cut(s)	593, 655, 782, 902, 962, 1166, 1938, 2033
FalI	AAGNNNNNCTT	4 cut(s)	407, 439, 1065, 1097
FaqI	GGGAC	2 cut(s)	835, 1240
FatI	CATG	8 cut(s)	589, 651, 778, 898, 958, 1162, 1934, 2029
FauI	CCCGC	1 cut(s)	594
FblI	GTMKAC	1 cut(s)	390
FokI	GGATG	4 cut(s)	686, 1346, 1496, 1553
FspBI	CTAG	5 cut(s)	863, 951, 1007, 1859, 1875
GlaI	GCGC	2 cut(s)	1581, 1895
GsaI	CCCAGC	2 cut(s)	413, 1900
GsuI	CTGGAG	1 cut(s)	1095
HaeII	RGCGCY	1 cut(s)	1897
HapII	CCGG	5 cut(s)	5, 69, 210, 891, 1650
HgaI	GACGC	2 cut(s)	74, 2008
HhaI	GCGC	2 cut(s)	1582, 1896
Hin1I	GRCGYC	1 cut(s)	66
Hin1II	CATG	8 cut(s)	593, 655, 782, 902, 962, 1166, 1938, 2033
Hin6I	GCGC	2 cut(s)	1580, 1894
HinP1I	GCGC	2 cut(s)	1580, 1894
HindIII	AAGCTT	2 cut(s)	664, 1280
HinfI	GANTC	5 cut(s)	55, 181, 1001, 1077, 1901
HpaII	CCGG	5 cut(s)	5, 69, 210, 891, 1650
HphI	GGTGA	4 cut(s)	178, 224, 655, 1180
Hpy166II	GTNNAC	6 cut(s)	391, 607, 1136, 1171, 1274, 2027
Hpy188I	TCNGA	5 cut(s)	60, 186, 932, 1407, 1843
Hpy188III	TCNNGA	7 cut(s)	359, 765, 998, 1112, 1244, 1905, 1982
Hpy8I	GTNNAC	6 cut(s)	391, 607, 1136, 1171, 1274, 2027
Hpy99I	CGWCG	1 cut(s)	68
HpyCH4III	ACNGT	5 cut(s)	296, 373, 940, 1231, 2089
HpyCH4IV	ACGT	3 cut(s)	943, 1106, 1781
HpyF3I	CTNAG	4 cut(s)	311, 360, 1458, 1958
HpySE526I	ACGT	3 cut(s)	943, 1106, 1781
Hsp92I	GRCGYC	1 cut(s)	66
Hsp92II	CATG	8 cut(s)	593, 655, 782, 902, 962, 1166, 1938, 2033
HspAI	GCGC	2 cut(s)	1580, 1894
KspI	CCGCGG	1 cut(s)	602
Kzo9I	GATC	5 cut(s)	60, 853, 994, 1386, 1939
LmnI	GCTCC	5 cut(s)	29, 355, 488, 1612, 1652
Lsp1109I	GCAGC	6 cut(s)	44, 184, 354, 597, 1717, 1940
LweI	GCATC	3 cut(s)	747, 1531, 2075
MaeI	CTAG	5 cut(s)	863, 951, 1007, 1859, 1875
MaeII	ACGT	3 cut(s)	943, 1106, 1781
MaeIII	GTNAC	4 cut(s)	49, 367, 944, 1777
MalI	GATC	5 cut(s)	62, 855, 996, 1388, 1941
MbiI	CCGCTC	1 cut(s)	107
MboI	GATC	5 cut(s)	60, 853, 994, 1386, 1939
MhlI	GDGCHC	2 cut(s)	609, 1276
MlsI	TGGCCA	2 cut(s)	704, 1790
MluCI	AATT	7 cut(s)	538, 1151, 1195, 1288, 1356, 1519, 1834
MluNI	TGGCCA	2 cut(s)	704, 1790
MlyI	GAGTC	1 cut(s)	1010
Mox20I	TGGCCA	2 cut(s)	704, 1790
Mph1103I	ATGCAT	1 cut(s)	762
MroXI	GAANNNNTTC	1 cut(s)	801
MscI	TGGCCA	2 cut(s)	704, 1790
MseI	TTAA	6 cut(s)	234, 506, 1287, 1316, 1740, 1749
MslI	CAYNNNNRTG	6 cut(s)	556, 710, 783, 1434, 1741, 1817
Msp20I	TGGCCA	2 cut(s)	704, 1790
MspA1I	CMGCKG	1 cut(s)	601
MspI	CCGG	5 cut(s)	5, 69, 210, 891, 1650
MspR9I	CCNGG	5 cut(s)	486, 819, 1048, 1061, 1994
Mva1269I	GAATGC	1 cut(s)	760
MvaI	CCWGG	5 cut(s)	486, 819, 1048, 1061, 1994
MvnI	CGCG	1 cut(s)	601
NcoI	CCATGG	1 cut(s)	589
NdeII	GATC	5 cut(s)	60, 853, 994, 1386, 1939
NlaIII	CATG	8 cut(s)	593, 655, 782, 902, 962, 1166, 1938, 2033
NlaIV	GGNNCC	3 cut(s)	23, 382, 660
NotI	GCGGCCGC	1 cut(s)	120
NsiI	ATGCAT	1 cut(s)	762
NspI	RCATGY	5 cut(s)	655, 902, 962, 1166, 2033
OliI	CACNNNNGTG	1 cut(s)	556
PaeI	GCATGC	1 cut(s)	962
PaeR7I	CTCGAG	1 cut(s)	765
PciI	ACATGT	3 cut(s)	651, 1162, 2029
PctI	GAATGC	1 cut(s)	760
PdmI	GAANNNNTTC	1 cut(s)	801
PfeI	GAWTC	4 cut(s)	55, 181, 1077, 1901
PinAI	ACCGGT	1 cut(s)	890
Ple19I	CGATCG	1 cut(s)	63
PleI	GAGTC	1 cut(s)	1009
PpsI	GAGTC	1 cut(s)	1009
PscI	ACATGT	3 cut(s)	651, 1162, 2029
PshBI	ATTAAT	1 cut(s)	1287
PsiI	TTATAA	1 cut(s)	1398
Psp6I	CCWGG	5 cut(s)	484, 817, 1046, 1059, 1992
PspFI	CCCAGC	2 cut(s)	409, 1896
PspGI	CCWGG	5 cut(s)	484, 817, 1046, 1059, 1992
PspN4I	GGNNCC	3 cut(s)	23, 382, 660
PspPI	GGNCC	7 cut(s)	22, 482, 545, 659, 918, 1527, 1809
PstI	CTGCAG	2 cut(s)	1798, 1930
PstNI	CAGNNNCTG	2 cut(s)	491, 1550
PvuI	CGATCG	1 cut(s)	63
RsaI	GTAC	7 cut(s)	9, 777, 814, 942, 1693, 2008, 2026
RsaNI	GTAC	7 cut(s)	8, 776, 813, 941, 1692, 2007, 2025
RseI	CAYNNNNRTG	6 cut(s)	556, 710, 783, 1434, 1741, 1817
SacII	CCGCGG	1 cut(s)	602
SaqAI	TTAA	6 cut(s)	234, 506, 1287, 1316, 1740, 1749
Sau3AI	GATC	5 cut(s)	60, 853, 994, 1386, 1939
Sau96I	GGNCC	7 cut(s)	22, 482, 545, 659, 918, 1527, 1809
SchI	GAGTC	1 cut(s)	1010
ScrFI	CCNGG	5 cut(s)	486, 819, 1048, 1061, 1994
SduI	GDGCHC	2 cut(s)	609, 1276
SfaNI	GCATC	3 cut(s)	747, 1531, 2075
SfcI	CTRYAG	3 cut(s)	391, 1794, 1926
Sfr274I	CTCGAG	1 cut(s)	765
Sfr303I	CCGCGG	1 cut(s)	602
SgrBI	CCGCGG	1 cut(s)	602
SinI	GGWCC	2 cut(s)	482, 545
SlaI	CTCGAG	1 cut(s)	765
SmiMI	CAYNNNNRTG	6 cut(s)	556, 710, 783, 1434, 1741, 1817
SmlI	CTYRAG	3 cut(s)	765, 998, 1980
SmoI	CTYRAG	3 cut(s)	765, 998, 1980
SphI	GCATGC	1 cut(s)	962
Sse9I	AATT	7 cut(s)	538, 1151, 1195, 1288, 1356, 1519, 1834
SspI	AATATT	1 cut(s)	747
SspMI	CTAG	5 cut(s)	863, 951, 1007, 1859, 1875
StyD4I	CCNGG	5 cut(s)	484, 817, 1046, 1059, 1992
StyI	CCWWGG	4 cut(s)	324, 589, 1686, 1967
TaaI	ACNGT	5 cut(s)	296, 373, 940, 1231, 2089
TaiI	ACGT	3 cut(s)	946, 1109, 1784
TaqI	TCGA	3 cut(s)	63, 513, 766
TaqII	GACCGA	2 cut(s)	431, 1426
TasI	AATT	7 cut(s)	538, 1151, 1195, 1288, 1356, 1519, 1834
TatI	WGTACW	2 cut(s)	775, 2024
TfiI	GAWTC	4 cut(s)	55, 181, 1077, 1901
Tru1I	TTAA	6 cut(s)	234, 506, 1287, 1316, 1740, 1749
Tru9I	TTAA	6 cut(s)	234, 506, 1287, 1316, 1740, 1749
TseI	GCWGC	6 cut(s)	32, 197, 342, 585, 1705, 1928
TspDTI	ATGAA	7 cut(s)	302, 1200, 1353, 1430, 1587, 1659, 2001
TspGWI	ACGGA	1 cut(s)	1022
VneI	GTGCAC	2 cut(s)	605, 1272
VpaK11BI	GGWCC	2 cut(s)	482, 545
VspI	ATTAAT	1 cut(s)	1287
XagI	CCTNNNNNAGG	1 cut(s)	1878
XapI	RAATTY	1 cut(s)	1519
XceI	RCATGY	5 cut(s)	655, 902, 962, 1166, 2033
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1380
XhoI	CTCGAG	1 cut(s)	765
XmiI	GTMKAC	1 cut(s)	390
XmnI	GAANNNNTTC	1 cut(s)	801
XspI	CTAG	5 cut(s)	863, 951, 1007, 1859, 1875
Zsp2I	ATGCAT	1 cut(s)	762

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.