Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0002179

Physical Location & Seq

Genomic Coordinates Reverse (-)

21875962 .. 21881686

Gene Structure 5725 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1184900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2127 bp

ATGCCGGTACCGCGTGGCGGAGCACGCAGGGGACGAGGAGCAAAGGCGAAAGCAGCTAAGAAGCAGAAACAGGAGAAGGTTTCGAGTCCGATCGACGCCGGCGAGGCGATAGCCACGAGAACAAGGCGGAGGAGAGCGGCGGCGGCGGCAGCAGCAGCTGTGCCGAGTAATCTCAATAACAAGAACGTGAGTGATAAGGTTAGAGAAGAGGAGCAGAATCGCGAGAAGGTGGTGGTGGTGGGTGATGAGAAAAACGTAGTCGTTGAGGCAGAGAAGGAGAAATTGGGAGAGAAGAAAATGGATGACTATGATAGTGGCGGCGGAGCAGCCGGCGCTGCGCCGAGCAATGATAAGGCCAATGCCGGTGAAGACGAAGGAAGCACTGCTCCAATTCCTGAAAAGGTTCAGGTTGGTGGTTCCCCTGTTTACAAAGTGGACAGAAAGCTGGGTAAGGGTGGTTTCGGACAAGTCTATGTTGGTCGACGTGTAATAAATACAAACGAGAGAACTGGCCCAGGAGCTTTAGAGGTGGCATTGAAATTTGAGCATAAAAATAGTAAAGGGTGTAGCTATGGACCACCAGCTGAGTGGCAAATATACAGCACTCTTGGAGGCAGCCATGGCGTGCCACGAGTACATTTCAAGGGCCAGCAAGGCGACTATTATGTCATGGTTATGGATATCTTGGGCCCAAGCTTGTGGGATGTTTGGAATAATAACTCTCACACAATGTCTGTTGAAATGGTTGCATGCATTGCCATTGAAGCAATATCAATATTGGAGAAGACGCATTCTCGAGGGTATGTGCATGGGGATGTAAAGCCTGAGAATTTTCTGCTTGGTCCTCCTGGGACTCCCGATGAGAAAAAGTTGTATCTAGTTGACCTTGGACTAGCAACTAGATGGCGAGAAAGTACAAGTGGTCAGCATGTAGAGTATGACCAGAGGCCAGATGTTTTCAGAGGAACAGTGCGTTATGCTAGTGTGCATGCGCATTTAGGTAGAACGGGTAGTAGGAGAGATGATCTCGAGTCTCTAGCTTACACCCTTGTATTCCTTCTACGTGGTCGTCTGCCTTGGCAAGGATATCAGGGAGACAATAAAGGGTTTCTTGTTTGCAAAAAGAAGATGGCAACATCTCCAGAGACATTGTGTTCCTTCAATCCAAAGCCGTTTAGACAATTTGTGGAATATGTGGTGAACTTGAAGTTCGATGAAGAGCCAAATTATGCCAAATATATATCACTCTTTGATGGCGTTGTTGGTCCAAATCCTGCTATCAGGCCAATAAACACAGAGGGTGCACAGAAGCTTATTAATCTGGTCGGTCATAAAAGAGGAAGATTGAGTATGGAGGAGGAAGACGATGAACAACCGAAGAAGAGGATTAGGATGGGTATGCCAGCAAATCAGTGGATTAGTGTTTATAATGCTCATAGACCAATGAAGCAAAGGTATCACTATAATGTGGCTGATACTAGGCTTGCTCAGCATATTGATAAAGGGACCGAGGATGGGTTATATATTAGCAGTGTGGCCTCTTATGAAAATTTGTGGGCCTTAATTATGGATGCAGGGACTAATTTTTCTTCCCAAGTTTACGAACTCTCACCATGTTCTCTTCACAAGGAATGGATAATGGAGCAGTGGGATAAGAACTATTACATCAGTGCGATAGCAGGAGCTACCAATGGGAGTTCATTAGTAGTAATGTCCAAGGGTACAACGTTTACGCAGCAGTCATACAAAGTAAGCGATACATTTCCGTATAAGTGGATTAACAAAAAGTGGAGGGAGGGTTTCCATGTTACTGCAATGGCCACTTCTGGAAGTAGATGGGCAGTTGTTATGTCTCGTGGTGCAAATTATACAGACCAGGTTGTTGAGCTAGATTTTCTTTATCCAAGCGAAGGTATTCATAGGCGGTGGGACAGTGGATATCGCATAACAGCAACTGCAGCAACGTGTGATCAGGCTGCTTTTGTTCTTAGTATCCCAAAGAGGAAGCTTCAAGACGAAACACAGGAGACGCTTCGTACTTCTGTATTTCCCAGTACGCACGTCAAGGAGAAGTGGGCAAAGAACCTTTATCTTGCATCCATTTGTTACGGGCGAACAGTTTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0030100 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0032879 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0060627 GO:0065007 GO:0071704 GO:0097159 GO:0097367 GO:0098657 GO:0140096 GO:1901265 GO:1901363 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

709

Amino Acids

78.92

Weight (kDa)

9.17

Isoelectric Point (pI)

41.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	143 - 378	1.9e-16	Protein kinase domain
Pkinase_fungal	PF17667	247 - 352	2.2e-06	Fungal protein kinase
DUF7477	PF24289	442 - 708	3.8e-160	Domain of unknown function (DUF7477)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002179)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25760 AT2G25760
fragaria_vesca	FvH4_2g35440 FvH4_2g35440 FvH4_6g32280
malus_domestica	MD08G1111700.v1.1 MD09G1204700.v1.1 MD15G1091200.v1.1 MD17G1184900.v1.1
prunus_persica	Prupe.1G445600_v2.0.a1 Prupe.1G445600_v2.0.a1 Prupe.1G445600_v2.0.a1 Prupe.1G445600_v2.0.a1 Prupe.1G445600_v2.0.a1 Prupe.3G060400_v2.0.a1 Prupe.3G060400_v2.0.a1
pyrus_communis	pycom08g09280 pycom09g12160 pycom15g08520 pycom17g19440
rosa_chinensis	RchiOBHm_Chr2g0140231 RchiOBHm_Chr6g0306451
rosa_laevigata	RLG00000004784 RLG00000008671 RLG00000010797 RLG00000019857
rosa_multiflora	Rmu_sc0002971.1_g000017 Rmu_sc0005446.1_g000008
rosa_roxburghii	Rroxscaffold_2G00104970 Rroxscaffold_7G00161760
rosa_rugosa	Rorug02G0361500 Rorug06G0349500 Rorug06G0349600
rosa_samantha	Rh2AG412400 Rh2BG422700 Rh2CG398600 Rh6AG461700 Rh6BG439300 Rh6CG475800 Rh6DG462500
rosa_wichuraiana	Rw2G033800 Rw6G040240 Rw7G007580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1428
AasI	GACNNNNNNGTC	1 cut(s)	665
Acc16I	TGCGCA	1 cut(s)	993
Acc65I	GGTACC	1 cut(s)	7
AccB1I	GGYRCC	1 cut(s)	7
AccBSI	CCGCTC	1 cut(s)	137
AccI	GTMKAC	1 cut(s)	481
AccII	CGCG	2 cut(s)	13, 222
AclI	AACGTT	1 cut(s)	1727
AcoI	YGGCCR	1 cut(s)	1818
AcsI	RAATTY	3 cut(s)	539, 829, 1549
AcyI	GRCGYC	1 cut(s)	96
AfaI	GTAC	6 cut(s)	9, 636, 916, 1723, 2038, 2056
AfiI	CCNNNNNNNGG	4 cut(s)	17, 1082, 1515, 1910
AflIII	ACRYGT	2 cut(s)	484, 1964
AgsI	TTSAA	7 cut(s)	538, 643, 740, 764, 1162, 1207, 2012
AjiI	CACGTC	2 cut(s)	485, 2062
AjnI	CCWGG	3 cut(s)	514, 847, 1875
AjuI	GAANNNNNNNTTGG	2 cut(s)	266, 298
Alw21I	GWGCWC	2 cut(s)	25, 1306
Alw26I	GTCTC	5 cut(s)	1038, 1089, 1139, 1857, 2021
Alw44I	GTGCAC	1 cut(s)	1302
AlwNI	CAGNNNCTG	2 cut(s)	158, 1955
Ama87I	CYCGRG	2 cut(s)	795, 1028
AoxI	GGCC	9 cut(s)	354, 511, 646, 688, 947, 1283, 1536, 1557, 1818
ApaI	GGGCCC	1 cut(s)	692
ApaLI	GTGCAC	1 cut(s)	1302
ApoI	RAATTY	3 cut(s)	539, 829, 1549
AseI	ATTAAT	1 cut(s)	1317
Asp700I	GAANNNNTTC	3 cut(s)	402, 1914, 2119
Asp718I	GGTACC	1 cut(s)	7
AspLEI	GCGC	3 cut(s)	335, 340, 994
AspS9I	GGNCC	9 cut(s)	512, 575, 646, 688, 689, 842, 1265, 1506, 1557
AsuHPI	GGTGA	4 cut(s)	254, 377, 1210, 1602
AvaI	CYCGRG	2 cut(s)	795, 1028
AvaII	GGWCC	4 cut(s)	575, 842, 1265, 1506
BaeGI	GKGCMC	2 cut(s)	692, 1306
BalI	TGGCCA	1 cut(s)	1820
BanI	GGYRCC	1 cut(s)	7
BanII	GRGCYC	1 cut(s)	692
BauI	CACGAG	3 cut(s)	115, 630, 1854
BbsI	GAAGAC	3 cut(s)	375, 791, 1368
Bbv12I	GWGCWC	2 cut(s)	25, 1306
BccI	CCATC	6 cut(s)	897, 1123, 1247, 1387, 1508, 1830
BceAI	ACGGC	1 cut(s)	1156
BciT130I	CCWGG	3 cut(s)	516, 849, 1877
BciVI	GTATCC	1 cut(s)	2003
BclI	TGATCA	1 cut(s)	1969
BcoDI	GTCTC	5 cut(s)	1038, 1089, 1139, 1857, 2021
BfaI	CTAG	7 cut(s)	878, 893, 900, 981, 1037, 1479, 1889
BfmI	CTRYAG	1 cut(s)	1956
BfoI	RGCGCY	1 cut(s)	336
BfuI	GTATCC	1 cut(s)	2003
BglI	GCCNNNNNGGC	2 cut(s)	104, 654
BlpI	GCTNAGC	1 cut(s)	1488
Bme1390I	CCNGG	3 cut(s)	516, 849, 1877
Bme18I	GGWCC	4 cut(s)	575, 842, 1265, 1506
BmeT110I	CYCGRG	2 cut(s)	795, 1028
BmgBI	CACGTC	2 cut(s)	485, 2062
BmgT120I	GGNCC	9 cut(s)	512, 575, 646, 688, 689, 842, 1265, 1506, 1557
BmiI	GGNNCC	4 cut(s)	9, 418, 690, 1507
BmrFI	CCNGG	3 cut(s)	516, 849, 1877
BmrI	ACTGGG	1 cut(s)	2046
BmsI	GCATC	2 cut(s)	1561, 2105
BmuI	ACTGGG	1 cut(s)	2046
BpiI	GAAGAC	3 cut(s)	375, 791, 1368
BplI	GAGNNNNNCTC	2 cut(s)	1011, 1043
BpmI	CTGGAG	1 cut(s)	1125
Bpu1102I	GCTNAGC	1 cut(s)	1488
BsaAI	YACGTR	1 cut(s)	1064
BsaHI	GRCGYC	1 cut(s)	96
BsaJI	CCNNGG	7 cut(s)	514, 619, 848, 886, 1076, 1509, 1716
BsaXI	ACNNNNNCTCC	2 cut(s)	1783, 1813
Bsc4I	CCNNNNNNNGG	4 cut(s)	17, 1082, 1515, 1910
Bse118I	RCCGGY	4 cut(s)	4, 98, 329, 362
Bse1I	ACTGG	2 cut(s)	514, 2052
Bse3DI	GCAATG	3 cut(s)	352, 753, 1821
BseBI	CCWGG	3 cut(s)	516, 849, 1877
BseDI	CCNNGG	7 cut(s)	514, 619, 848, 886, 1076, 1509, 1716
BseGI	GGATG	7 cut(s)	307, 709, 820, 1398, 1519, 1576, 2096
BseLI	CCNNNNNNNGG	4 cut(s)	17, 1082, 1515, 1910
BseMI	GCAATG	3 cut(s)	352, 753, 1821
BseMII	CTCAG	3 cut(s)	576, 816, 1502
BseNI	ACTGG	2 cut(s)	514, 2052
BseRI	GAGGAG	4 cut(s)	51, 145, 224, 1370
BseSI	GKGCMC	2 cut(s)	692, 1306
BseYI	CCCAGC	1 cut(s)	445
Bsh1236I	CGCG	2 cut(s)	13, 222
Bsh1285I	CGRYCG	1 cut(s)	93
BshFI	GGCC	9 cut(s)	356, 513, 648, 690, 949, 1285, 1538, 1559, 1820
BshNI	GGYRCC	1 cut(s)	7
BsiEI	CGRYCG	1 cut(s)	93
BsiHKAI	GWGCWC	2 cut(s)	25, 1306
BsiHKCI	CYCGRG	2 cut(s)	795, 1028
BsiSI	CCGG	4 cut(s)	5, 99, 330, 363
BslFI	GGGAC	5 cut(s)	45, 865, 1519, 1591, 1943
BslI	CCNNNNNNNGG	4 cut(s)	17, 1082, 1515, 1910
BsmAI	GTCTC	5 cut(s)	1038, 1089, 1139, 1857, 2021
BsmBI	CGTCTC	1 cut(s)	2021
BsmFI	GGGAC	5 cut(s)	45, 865, 1519, 1591, 1943
BsmI	GAATGC	1 cut(s)	790
BsnI	GGCC	9 cut(s)	356, 513, 648, 690, 949, 1285, 1538, 1559, 1820
BsoBI	CYCGRG	2 cut(s)	795, 1028
Bsp120I	GGGCCC	1 cut(s)	688
Bsp1286I	GDGCHC	3 cut(s)	25, 692, 1306
Bsp143I	GATC	3 cut(s)	90, 1024, 1969
Bsp1720I	GCTNAGC	1 cut(s)	1488
Bsp19I	CCATGG	1 cut(s)	619
Bsp68I	TCGCGA	1 cut(s)	222
BspANI	GGCC	9 cut(s)	356, 513, 648, 690, 949, 1285, 1538, 1559, 1820
BspCNI	CTCAG	3 cut(s)	577, 817, 1501
BspFNI	CGCG	2 cut(s)	13, 222
BspLI	GGNNCC	4 cut(s)	9, 418, 690, 1507
BspMAI	CTGCAG	1 cut(s)	1960
BspQI	GCTCTTC	1 cut(s)	1212
BspT107I	GGYRCC	1 cut(s)	7
BsrBI	CCGCTC	1 cut(s)	137
BsrDI	GCAATG	3 cut(s)	352, 753, 1821
BsrFI	RCCGGY	4 cut(s)	4, 98, 329, 362
BsrI	ACTGG	2 cut(s)	514, 2052
BssAI	RCCGGY	4 cut(s)	4, 98, 329, 362
BssECI	CCNNGG	7 cut(s)	514, 619, 848, 886, 1076, 1509, 1716
BssMI	GATC	3 cut(s)	90, 1024, 1969
BssNI	GRCGYC	1 cut(s)	96
BssSI	CACGAG	3 cut(s)	115, 630, 1854
BssT1I	CCWWGG	4 cut(s)	619, 886, 1076, 1716
Bst2BI	CACGAG	3 cut(s)	115, 630, 1854
Bst2UI	CCWGG	3 cut(s)	516, 849, 1877
Bst4CI	ACNGT	3 cut(s)	970, 1934, 2119
Bst6I	CTCTTC	4 cut(s)	201, 1212, 1376, 1626
BstACI	GRCGYC	1 cut(s)	96
BstAPI	GCANNNNNTGC	1 cut(s)	755
BstBAI	YACGTR	1 cut(s)	1064
BstC8I	GCNNGC	9 cut(s)	25, 100, 331, 626, 650, 751, 990, 1404, 1485
BstDEI	CTNAG	5 cut(s)	57, 585, 825, 1488, 1988
BstDSI	CCRYGG	1 cut(s)	619
BstENI	CCTNNNNNAGG	1 cut(s)	1080
BstF5I	GGATG	7 cut(s)	307, 709, 820, 1398, 1519, 1576, 2096
BstFNI	CGCG	2 cut(s)	13, 222
BstH2I	RGCGCY	1 cut(s)	336
BstHHI	GCGC	3 cut(s)	335, 340, 994
BstKTI	GATC	3 cut(s)	93, 1027, 1972
BstMAI	GTCTC	5 cut(s)	1038, 1089, 1139, 1857, 2021
BstMBI	GATC	3 cut(s)	90, 1024, 1969
BstMCI	CGRYCG	1 cut(s)	93
BstNI	CCWGG	3 cut(s)	516, 849, 1877
BstNSI	RCATGY	3 cut(s)	753, 932, 992
BstSCI	CCNGG	3 cut(s)	514, 847, 1875
BstSFI	CTRYAG	1 cut(s)	1956
BstSLI	GKGCMC	2 cut(s)	692, 1306
BstUI	CGCG	2 cut(s)	13, 222
BstV2I	GAAGAC	3 cut(s)	375, 791, 1368
BstXI	CCANNNNNNTGG	2 cut(s)	588, 699
BsuI	GTATCC	1 cut(s)	2003
BsuRI	GGCC	9 cut(s)	356, 513, 648, 690, 949, 1285, 1538, 1559, 1820
BtgI	CCRYGG	1 cut(s)	619
BtrI	CACGTC	2 cut(s)	485, 2062
BtsCI	GGATG	7 cut(s)	307, 709, 820, 1398, 1519, 1576, 2096
BtsI	GCAGTG	3 cut(s)	381, 1537, 1652
BtsIMutI	CAGTG	7 cut(s)	381, 975, 1418, 1537, 1652, 1675, 1939
BtuMI	TCGCGA	1 cut(s)	222
Cac8I	GCNNGC	9 cut(s)	25, 100, 331, 626, 650, 751, 990, 1404, 1485
CaiI	CAGNNNCTG	2 cut(s)	158, 1955
CfoI	GCGC	3 cut(s)	335, 340, 994
Cfr10I	RCCGGY	4 cut(s)	4, 98, 329, 362
Cfr13I	GGNCC	9 cut(s)	512, 575, 646, 688, 689, 842, 1265, 1506, 1557
CseI	GACGC	3 cut(s)	104, 796, 2038
CsiI	ACCWGGT	1 cut(s)	1875
Csp6I	GTAC	6 cut(s)	8, 635, 915, 1722, 2037, 2055
CviAII	CATG	8 cut(s)	620, 670, 750, 809, 929, 989, 1614, 1805
CviQI	GTAC	6 cut(s)	8, 635, 915, 1722, 2037, 2055
DdeI	CTNAG	5 cut(s)	57, 585, 825, 1488, 1988
DpnI	GATC	3 cut(s)	92, 1026, 1971
DpnII	GATC	3 cut(s)	90, 1024, 1969
DrdI	GACNNNNNNGTC	1 cut(s)	665
DseDI	GACNNNNNNGTC	1 cut(s)	665
EaeI	YGGCCR	1 cut(s)	1818
Eam1104I	CTCTTC	4 cut(s)	201, 1212, 1376, 1626
EarI	CTCTTC	4 cut(s)	201, 1212, 1376, 1626
EciI	GGCGGA	3 cut(s)	33, 142, 336
Eco130I	CCWWGG	4 cut(s)	619, 886, 1076, 1716
Eco24I	GRGCYC	1 cut(s)	692
Eco32I	GATATC	3 cut(s)	682, 1088, 1940
Eco47I	GGWCC	4 cut(s)	575, 842, 1265, 1506
Eco88I	CYCGRG	2 cut(s)	795, 1028
EcoNI	CCTNNNNNAGG	1 cut(s)	1080
EcoRII	CCWGG	3 cut(s)	514, 847, 1875
EcoRV	GATATC	3 cut(s)	682, 1088, 1940
EcoT14I	CCWWGG	4 cut(s)	619, 886, 1076, 1716
EcoT22I	ATGCAT	1 cut(s)	755
EcoT38I	GRGCYC	1 cut(s)	692
ErhI	CCWWGG	4 cut(s)	619, 886, 1076, 1716
Esp3I	CGTCTC	1 cut(s)	2021
FaeI	CATG	8 cut(s)	623, 673, 753, 812, 932, 992, 1617, 1808
FalI	AAGNNNNNCTT	2 cut(s)	1095, 1127
FaqI	GGGAC	5 cut(s)	45, 865, 1519, 1591, 1943
FatI	CATG	8 cut(s)	619, 669, 749, 808, 928, 988, 1613, 1804
FbaI	TGATCA	1 cut(s)	1969
FblI	GTMKAC	1 cut(s)	481
FokI	GGATG	7 cut(s)	314, 716, 827, 1405, 1526, 1583, 2083
FriOI	GRGCYC	1 cut(s)	692
FspAI	RTGCGCAY	1 cut(s)	993
FspBI	CTAG	7 cut(s)	878, 893, 900, 981, 1037, 1479, 1889
FspI	TGCGCA	1 cut(s)	993
GlaI	GCGC	3 cut(s)	334, 339, 993
GsaI	CCCAGC	1 cut(s)	449
GsuI	CTGGAG	1 cut(s)	1125
HaeII	RGCGCY	1 cut(s)	336
HaeIII	GGCC	9 cut(s)	356, 513, 648, 690, 949, 1285, 1538, 1559, 1820
HapII	CCGG	4 cut(s)	5, 99, 330, 363
HgaI	GACGC	3 cut(s)	104, 796, 2038
HhaI	GCGC	3 cut(s)	335, 340, 994
Hin1I	GRCGYC	1 cut(s)	96
Hin1II	CATG	8 cut(s)	623, 673, 753, 812, 932, 992, 1617, 1808
Hin6I	GCGC	3 cut(s)	333, 338, 992
HinP1I	GCGC	3 cut(s)	333, 338, 992
HincII	GTYRAC	2 cut(s)	482, 883
HindII	GTYRAC	2 cut(s)	482, 883
HindIII	AAGCTT	3 cut(s)	694, 1310, 2006
HinfI	GANTC	4 cut(s)	85, 217, 853, 1031
HpaII	CCGG	4 cut(s)	5, 99, 330, 363
HphI	GGTGA	4 cut(s)	254, 377, 1210, 1602
Hpy166II	GTNNAC	8 cut(s)	427, 436, 482, 883, 1201, 1304, 1600, 1731
Hpy188I	TCNGA	3 cut(s)	90, 464, 962
Hpy188III	TCNNGA	8 cut(s)	221, 395, 795, 857, 1028, 1142, 1827, 2012
Hpy8I	GTNNAC	8 cut(s)	427, 436, 482, 883, 1201, 1304, 1600, 1731
Hpy99I	CGWCG	2 cut(s)	98, 486
HpyAV	CCTTC	7 cut(s)	70, 220, 268, 368, 1067, 1168, 1904
HpyCH4III	ACNGT	3 cut(s)	970, 1934, 2119
HpyCH4IV	ACGT	7 cut(s)	186, 255, 484, 1063, 1727, 1964, 2061
HpyF3I	CTNAG	5 cut(s)	57, 585, 825, 1488, 1988
HpySE526I	ACGT	7 cut(s)	186, 255, 484, 1063, 1727, 1964, 2061
Hsp92I	GRCGYC	1 cut(s)	96
Hsp92II	CATG	8 cut(s)	623, 673, 753, 812, 932, 992, 1617, 1808
HspAI	GCGC	3 cut(s)	333, 338, 992
KpnI	GGTACC	1 cut(s)	11
KroI	GCCGGC	2 cut(s)	98, 329
KroNI	GCCGGC	2 cut(s)	100, 331
Ksp22I	TGATCA	1 cut(s)	1969
Kzo9I	GATC	3 cut(s)	90, 1024, 1969
LguI	GCTCTTC	1 cut(s)	1212
LmnI	GCTCC	8 cut(s)	20, 38, 211, 323, 391, 518, 1642, 1682
LweI	GCATC	2 cut(s)	1561, 2105
MabI	ACCWGGT	1 cut(s)	1875
MaeI	CTAG	7 cut(s)	878, 893, 900, 981, 1037, 1479, 1889
MaeII	ACGT	7 cut(s)	186, 255, 484, 1063, 1727, 1964, 2061
MaeIII	GTNAC	2 cut(s)	1807, 2105
MalI	GATC	3 cut(s)	92, 1026, 1971
MbiI	CCGCTC	1 cut(s)	137
MboI	GATC	3 cut(s)	90, 1024, 1969
MhlI	GDGCHC	3 cut(s)	25, 692, 1306
MlsI	TGGCCA	1 cut(s)	1820
MluNI	TGGCCA	1 cut(s)	1820
MlyI	GAGTC	3 cut(s)	94, 847, 1040
Mox20I	TGGCCA	1 cut(s)	1820
Mph1103I	ATGCAT	1 cut(s)	755
MreI	CGCCGGCG	1 cut(s)	98
MroNI	GCCGGC	2 cut(s)	98, 329
MroXI	GAANNNNTTC	3 cut(s)	402, 1914, 2119
MscI	TGGCCA	1 cut(s)	1820
MseI	TTAA	3 cut(s)	1317, 1562, 1779
MslI	CAYNNNNRTG	3 cut(s)	674, 813, 1464
Msp20I	TGGCCA	1 cut(s)	1820
MspA1I	CMGCKG	2 cut(s)	158, 584
MspI	CCGG	4 cut(s)	5, 99, 330, 363
MspR9I	CCNGG	3 cut(s)	516, 849, 1877
MteI	GCGCNGCGC	1 cut(s)	336
Mva1269I	GAATGC	1 cut(s)	790
MvaI	CCWGG	3 cut(s)	516, 849, 1877
MvnI	CGCG	2 cut(s)	13, 222
NaeI	GCCGGC	2 cut(s)	100, 331
NcoI	CCATGG	1 cut(s)	619
NdeII	GATC	3 cut(s)	90, 1024, 1969
NgoMIV	GCCGGC	2 cut(s)	98, 329
NlaIII	CATG	8 cut(s)	623, 673, 753, 812, 932, 992, 1617, 1808
NlaIV	GGNNCC	4 cut(s)	9, 418, 690, 1507
NmeAIII	GCCGAG	2 cut(s)	189, 366
NruI	TCGCGA	1 cut(s)	222
NsbI	TGCGCA	1 cut(s)	993
NsiI	ATGCAT	1 cut(s)	755
NspI	RCATGY	3 cut(s)	753, 932, 992
PaeI	GCATGC	2 cut(s)	753, 992
PaeR7I	CTCGAG	2 cut(s)	795, 1028
PciSI	GCTCTTC	1 cut(s)	1212
PcsI	WCGNNNNNNNCGW	2 cut(s)	31, 99
PctI	GAATGC	1 cut(s)	790
PdiI	GCCGGC	2 cut(s)	100, 331
PdmI	GAANNNNTTC	3 cut(s)	402, 1914, 2119
PfeI	GAWTC	1 cut(s)	217
Ple19I	CGATCG	1 cut(s)	93
PleI	GAGTC	3 cut(s)	93, 847, 1039
PpsI	GAGTC	3 cut(s)	93, 847, 1039
Ppu21I	YACGTR	1 cut(s)	1064
PshBI	ATTAAT	1 cut(s)	1317
PsiI	TTATAA	1 cut(s)	1428
Psp1406I	AACGTT	1 cut(s)	1727
Psp6I	CCWGG	3 cut(s)	514, 847, 1875
PspFI	CCCAGC	1 cut(s)	445
PspGI	CCWGG	3 cut(s)	514, 847, 1875
PspN4I	GGNNCC	4 cut(s)	9, 418, 690, 1507
PspOMI	GGGCCC	1 cut(s)	688
PspPI	GGNCC	9 cut(s)	512, 575, 646, 688, 689, 842, 1265, 1506, 1557
PstI	CTGCAG	1 cut(s)	1960
PstNI	CAGNNNCTG	2 cut(s)	158, 1955
PvuI	CGATCG	1 cut(s)	93
PvuII	CAGCTG	2 cut(s)	158, 584
RruI	TCGCGA	1 cut(s)	222
RsaI	GTAC	6 cut(s)	9, 636, 916, 1723, 2038, 2056
RsaNI	GTAC	6 cut(s)	8, 635, 915, 1722, 2037, 2055
RseI	CAYNNNNRTG	3 cut(s)	674, 813, 1464
SalI	GTCGAC	1 cut(s)	480
SapI	GCTCTTC	1 cut(s)	1212
SaqAI	TTAA	3 cut(s)	1317, 1562, 1779
Sau3AI	GATC	3 cut(s)	90, 1024, 1969
Sau96I	GGNCC	9 cut(s)	512, 575, 646, 688, 689, 842, 1265, 1506, 1557
SchI	GAGTC	3 cut(s)	94, 847, 1040
ScrFI	CCNGG	3 cut(s)	516, 849, 1877
SduI	GDGCHC	3 cut(s)	25, 692, 1306
SexAI	ACCWGGT	1 cut(s)	1875
SfaNI	GCATC	2 cut(s)	1561, 2105
SfcI	CTRYAG	1 cut(s)	1956
Sfr274I	CTCGAG	2 cut(s)	795, 1028
SgrAI	CRCCGGYG	1 cut(s)	98
SinI	GGWCC	4 cut(s)	575, 842, 1265, 1506
SlaI	CTCGAG	2 cut(s)	795, 1028
SmiMI	CAYNNNNRTG	3 cut(s)	674, 813, 1464
SmlI	CTYRAG	2 cut(s)	795, 1028
SmoI	CTYRAG	2 cut(s)	795, 1028
SphI	GCATGC	2 cut(s)	753, 992
SspI	AATATT	1 cut(s)	777
SspMI	CTAG	7 cut(s)	878, 893, 900, 981, 1037, 1479, 1889
StyD4I	CCNGG	3 cut(s)	514, 847, 1875
StyI	CCWWGG	4 cut(s)	619, 886, 1076, 1716
TaaI	ACNGT	3 cut(s)	970, 1934, 2119
TaiI	ACGT	7 cut(s)	189, 258, 487, 1066, 1730, 1967, 2064
TaqI	TCGA	6 cut(s)	83, 93, 481, 796, 1029, 1212
TaqII	GACCGA	2 cut(s)	1316, 1523
TatI	WGTACW	2 cut(s)	634, 914
TauI	GCSGC	5 cut(s)	140, 143, 146, 149, 321
TfiI	GAWTC	1 cut(s)	217
Tru1I	TTAA	3 cut(s)	1317, 1562, 1779
Tru9I	TTAA	3 cut(s)	1317, 1562, 1779
TscAI	CASTG	7 cut(s)	388, 975, 1418, 1537, 1652, 1675, 1939
TspDTI	ATGAA	7 cut(s)	1230, 1383, 1460, 1560, 1689, 1907, 2112
TspGWI	ACGGA	1 cut(s)	1755
TspRI	CASTG	7 cut(s)	388, 975, 1418, 1537, 1652, 1675, 1939
VneI	GTGCAC	1 cut(s)	1302
VpaK11BI	GGWCC	4 cut(s)	575, 842, 1265, 1506
VspI	ATTAAT	1 cut(s)	1317
XagI	CCTNNNNNAGG	1 cut(s)	1080
XapI	RAATTY	3 cut(s)	539, 829, 1549
XceI	RCATGY	3 cut(s)	753, 932, 992
XcmI	CCANNNNNNNNNTGG	2 cut(s)	585, 1410
XhoI	CTCGAG	2 cut(s)	795, 1028
XmiI	GTMKAC	1 cut(s)	481
XmnI	GAANNNNTTC	3 cut(s)	402, 1914, 2119
XspI	CTAG	7 cut(s)	878, 893, 900, 981, 1037, 1479, 1889
Zsp2I	ATGCAT	1 cut(s)	755

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.