pycom15g08520

Casein Kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002179
Physical Location & Seq
Reverse (-)
5340184 .. 5342266
2083 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g08520.1

Sequence Viewer

Length: 1200 bp
ATGTCGACTATGATCGAACGTCCAGATGTTTTCAGGGGAACGGTGCGGTATGCAAGTGTGCATGCTCATCTTGGTAGAACTGGTAGCAGGAGGGATGACTTGGAATCTCTAGCTTACACACTGATATTTCTTCTTCGTGGTCGGTTGCCATGGCAAGGATATCAGGGAGAAAATAAAGGATTTCTTGTCTGCAAGAAAAAGATGGCCACTTCCCCAGAAACTTTGTGTTGCTTCTGCCCTCAGCCATTCCGACAATTTGTTGAGTATGTGGTGAATTTGAAGTTTGATGAAGAACCCAACTATGCAAAGTACATATCCCTTTTTGATGGAATTGTTGGCCCAAACCCAGATATCAGGCCAATAAATACTGATGGTGCTCAGAAGCTTATATGCCAAGTTGGACAAAAGAGAGCAAGATTGGCCTTGGAGGAAGAGGATGATGAGCAACCAAAGAAGAAGATTCGTATGGGGATGCCTGCAACCCAATGGATTAGTGTATACAATGCTCGTAGGCCTATGAAGCAAAGATATCACTATAATGTTGCAGATGTGAGGCTCTCCCAACACATTGAGAAAGGAAATGAAGATGGGTTATTTATTAGCAGTGTGGCTTCCAGTTCAAATCTTTGGGCTCTTATCATGGATGCTGGCACTGGCTTCAGTGCCCAAGTTTATGAACTCTCTTCATATTTTCTGCATAAGGAATGGATTATGGAGCAGTGGGACAAGAATTACTATATCAGCGCGATAGCAGGAGCAAACAATGGAAGCTCTTTAGTTGTAATGTCTAAAGGGACCCAATACTTACAGCAGTCCTATAAAGTCAGCGATTCATTTCCTTTCAAGTGGATTAATAAGAAATGGCGGGAAGGATTTCATGTTACTGCAATGGCAACTGCTAGGAGTAGATGGGCTATTGTTATGTCCCGCGGTGCCGGATTTTCTGACCAGGTTGTGGAACTAGATTTTCTGTATCCAAGCGAAGGCATTCATCGAAGGTGGGATAATGGTTACCGCATCACGTCAACTGCAGCAACTTGGGATCAGGCAGCTTTTGTCCTTAGTGTTCCAAGGAGAAAACTGGCAGATGAAACTCAAGAGACGCTTCGGACTTCTGCTTTTCCTAGTACACACGTTAAGGAGAAATGGGGAAAAAACCTTTATATTGCATCTATTTCCTACGGTAGAACTGTCTCATGA

Protein Analysis

400

Amino Acids

45.67

Weight (kDa)

9.27

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7477 PF24289 132 - 399 1.3e-163 Domain of unknown function (DUF7477)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 932
AccB7I CCANNNNNTGG 1 cut(s) 955
AccI GTMKAC 2 cut(s) 5, 498
AccII CGCG 2 cut(s) 746, 930
AciI CCGC 5 cut(s) 46, 865, 928, 930, 1015
AclWI GGATC 1 cut(s) 1050
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 1 cut(s) 274
AcuI CTGAAG 1 cut(s) 643
AfaI GTAC 2 cut(s) 311, 1129
AfiI CCNNNNNNNGG 3 cut(s) 155, 955, 983
AflIII ACRYGT 1 cut(s) 1132
AgsI TTSAA 3 cut(s) 280, 621, 844
AjiI CACGTC 1 cut(s) 1023
AjnI CCWGG 1 cut(s) 948
AluBI AGCT 4 cut(s) 113, 385, 771, 1052
AluI AGCT 4 cut(s) 113, 385, 771, 1052
Alw21I GWGCWC 1 cut(s) 379
Alw26I GTCTC 1 cut(s) 1094
AlwI GGATC 1 cut(s) 1050
AoxI GGCC 5 cut(s) 204, 337, 356, 420, 512
ApeKI GCWGC 2 cut(s) 1031, 1049
ApoI RAATTY 1 cut(s) 274
AseI ATTAAT 1 cut(s) 852
Asp700I GAANNNNTTC 2 cut(s) 873, 987
AspLEI GCGC 1 cut(s) 746
AspS9I GGNCC 2 cut(s) 338, 795
AsuHPI GGTGA 1 cut(s) 283
AvaII GGWCC 1 cut(s) 795
BaeGI GKGCMC 1 cut(s) 667
BalI TGGCCA 1 cut(s) 206
BanI GGYRCC 1 cut(s) 932
BanII GRGCYC 1 cut(s) 634
Bbv12I GWGCWC 1 cut(s) 379
BbvCI CCTCAGC 1 cut(s) 240
BbvI GCAGC 2 cut(s) 1043, 1061
BccI CCATC 5 cut(s) 196, 320, 365, 581, 903
BciT130I CCWGG 1 cut(s) 950
BciVI GTATCC 1 cut(s) 984
BcoDI GTCTC 1 cut(s) 1094
BfaI CTAG 4 cut(s) 110, 900, 962, 1125
BfmI CTRYAG 1 cut(s) 1029
BfuI GTATCC 1 cut(s) 984
BisI GCNGC 2 cut(s) 1032, 1050
BlsI GCNGC 2 cut(s) 1033, 1051
Bme1390I CCNGG 1 cut(s) 950
Bme18I GGWCC 1 cut(s) 795
BmgBI CACGTC 1 cut(s) 1023
BmgT120I GGNCC 2 cut(s) 338, 795
BmiI GGNNCC 3 cut(s) 796, 797, 934
BmrFI CCNGG 1 cut(s) 950
BmsI GCATC 4 cut(s) 462, 634, 1026, 1178
Bpu10I CCTNAGC 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 1080
BsaJI CCNNGG 4 cut(s) 149, 423, 928, 1070
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 955, 983
Bse1I ACTGG 4 cut(s) 85, 615, 658, 1086
Bse3DI GCAATG 1 cut(s) 894
BseBI CCWGG 1 cut(s) 950
BseDI CCNNGG 4 cut(s) 149, 423, 928, 1070
BseGI GGATG 4 cut(s) 100, 442, 477, 649
BseLI CCNNNNNNNGG 3 cut(s) 155, 955, 983
BseMI GCAATG 1 cut(s) 894
BseMII CTCAG 2 cut(s) 254, 392
BseNI ACTGG 4 cut(s) 85, 615, 658, 1086
BseSI GKGCMC 1 cut(s) 667
BseXI GCAGC 2 cut(s) 1043, 1061
Bsh1236I CGCG 2 cut(s) 746, 930
BshFI GGCC 5 cut(s) 206, 339, 358, 422, 514
BshNI GGYRCC 1 cut(s) 932
BsiHKAI GWGCWC 1 cut(s) 379
BsiSI CCGG 1 cut(s) 936
BslFI GGGAC 3 cut(s) 737, 808, 910
BslI CCNNNNNNNGG 3 cut(s) 155, 955, 983
BsmAI GTCTC 1 cut(s) 1094
BsmBI CGTCTC 1 cut(s) 1094
BsmFI GGGAC 3 cut(s) 737, 808, 910
BsmI GAATGC 1 cut(s) 987
BsnI GGCC 5 cut(s) 206, 339, 358, 422, 514
Bsp1286I GDGCHC 3 cut(s) 379, 634, 667
Bsp143I GATC 2 cut(s) 12, 1042
Bsp19I CCATGG 1 cut(s) 149
BspACI CCGC 5 cut(s) 46, 865, 928, 930, 1015
BspANI GGCC 5 cut(s) 206, 339, 358, 422, 514
BspCNI CTCAG 2 cut(s) 253, 391
BspFNI CGCG 2 cut(s) 746, 930
BspHI TCATGA 1 cut(s) 1196
BspLI GGNNCC 3 cut(s) 796, 797, 934
BspMAI CTGCAG 1 cut(s) 1033
BspPI GGATC 1 cut(s) 1050
BspT107I GGYRCC 1 cut(s) 932
BsrDI GCAATG 1 cut(s) 894
BsrI ACTGG 4 cut(s) 85, 615, 658, 1086
BssECI CCNNGG 4 cut(s) 149, 423, 928, 1070
BssMI GATC 2 cut(s) 12, 1042
BssNAI GTATAC 1 cut(s) 499
BssT1I CCWWGG 3 cut(s) 149, 423, 1070
Bst1107I GTATAC 1 cut(s) 499
Bst2UI CCWGG 1 cut(s) 950
Bst4CI ACNGT 3 cut(s) 43, 1184, 1192
Bst6I CTCTTC 2 cut(s) 426, 688
BstC8I GCNNGC 3 cut(s) 63, 477, 649
BstDEI CTNAG 3 cut(s) 240, 378, 1061
BstDSI CCRYGG 2 cut(s) 149, 928
BstEII GGTNACC 1 cut(s) 1010
BstF5I GGATG 4 cut(s) 100, 442, 477, 649
BstFNI CGCG 2 cut(s) 746, 930
BstHHI GCGC 1 cut(s) 746
BstKTI GATC 2 cut(s) 15, 1045
BstMAI GTCTC 1 cut(s) 1094
BstMBI GATC 2 cut(s) 12, 1042
BstMWI GCNNNNNNNGC 2 cut(s) 419, 520
BstNI CCWGG 1 cut(s) 950
BstNSI RCATGY 1 cut(s) 65
BstPI GGTNACC 1 cut(s) 1010
BstSCI CCNGG 1 cut(s) 948
BstSFI CTRYAG 1 cut(s) 1029
BstSLI GKGCMC 1 cut(s) 667
BstUI CGCG 2 cut(s) 746, 930
BstV1I GCAGC 2 cut(s) 1043, 1061
BstZ17I GTATAC 1 cut(s) 499
BsuI GTATCC 1 cut(s) 984
BsuRI GGCC 5 cut(s) 206, 339, 358, 422, 514
BtgI CCRYGG 2 cut(s) 149, 928
BtrI CACGTC 1 cut(s) 1023
BtsCI GGATG 4 cut(s) 100, 442, 477, 649
BtsI GCAGTG 2 cut(s) 610, 725
BtsIMutI CAGTG 5 cut(s) 119, 610, 651, 667, 725
Cac8I GCNNGC 3 cut(s) 63, 477, 649
CciI TCATGA 1 cut(s) 1196
CfoI GCGC 1 cut(s) 746
Cfr13I GGNCC 2 cut(s) 338, 795
Cfr42I CCGCGG 1 cut(s) 931
CseI GACGC 1 cut(s) 1111
CsiI ACCWGGT 1 cut(s) 948
Csp6I GTAC 2 cut(s) 310, 1128
CviAII CATG 5 cut(s) 62, 150, 640, 878, 1197
CviQI GTAC 2 cut(s) 310, 1128
DdeI CTNAG 3 cut(s) 240, 378, 1061
DpnI GATC 2 cut(s) 14, 1044
DpnII GATC 2 cut(s) 12, 1042
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 2 cut(s) 426, 688
EarI CTCTTC 2 cut(s) 426, 688
Eco130I CCWWGG 3 cut(s) 149, 423, 1070
Eco147I AGGCCT 1 cut(s) 514
Eco24I GRGCYC 1 cut(s) 634
Eco32I GATATC 3 cut(s) 161, 352, 530
Eco47I GGWCC 1 cut(s) 795
Eco57I CTGAAG 1 cut(s) 643
Eco91I GGTNACC 1 cut(s) 1010
EcoO109I RGGNCCY 1 cut(s) 795
EcoO65I GGTNACC 1 cut(s) 1010
EcoRII CCWGG 1 cut(s) 948
EcoRV GATATC 3 cut(s) 161, 352, 530
EcoT14I CCWWGG 3 cut(s) 149, 423, 1070
EcoT38I GRGCYC 1 cut(s) 634
ErhI CCWWGG 3 cut(s) 149, 423, 1070
Esp3I CGTCTC 1 cut(s) 1094
FaeI CATG 5 cut(s) 65, 153, 643, 881, 1200
FalI AAGNNNNNCTT 4 cut(s) 168, 200, 1089, 1121
FaqI GGGAC 3 cut(s) 737, 808, 910
FatI CATG 5 cut(s) 61, 149, 639, 877, 1196
FauI CCCGC 2 cut(s) 858, 935
FblI GTMKAC 2 cut(s) 5, 498
Fnu4HI GCNGC 2 cut(s) 1032, 1050
FokI GGATG 4 cut(s) 107, 449, 484, 656
FriOI GRGCYC 1 cut(s) 634
Fsp4HI GCNGC 2 cut(s) 1032, 1050
FspBI CTAG 4 cut(s) 110, 900, 962, 1125
GlaI GCGC 1 cut(s) 745
GluI GCNGC 2 cut(s) 1032, 1050
HaeIII GGCC 5 cut(s) 206, 339, 358, 422, 514
HapII CCGG 1 cut(s) 936
HgaI GACGC 1 cut(s) 1111
HhaI GCGC 1 cut(s) 746
Hin1II CATG 5 cut(s) 65, 153, 643, 881, 1200
Hin6I GCGC 1 cut(s) 744
HinP1I GCGC 1 cut(s) 744
HincII GTYRAC 2 cut(s) 6, 1026
HindII GTYRAC 2 cut(s) 6, 1026
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 3 cut(s) 104, 460, 830
HpaII CCGG 1 cut(s) 936
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 4 cut(s) 6, 499, 1026, 1130
Hpy188I TCNGA 4 cut(s) 251, 381, 946, 1110
Hpy188III TCNNGA 3 cut(s) 23, 1097, 1197
Hpy8I GTNNAC 4 cut(s) 6, 499, 1026, 1130
HpyAV CCTTC 3 cut(s) 863, 977, 990
HpyCH4III ACNGT 3 cut(s) 43, 1184, 1192
HpyCH4IV ACGT 3 cut(s) 19, 1022, 1134
HpyF10VI GCNNNNNNNGC 2 cut(s) 419, 520
HpyF3I CTNAG 3 cut(s) 240, 378, 1061
HpySE526I ACGT 3 cut(s) 19, 1022, 1134
Hsp92II CATG 5 cut(s) 65, 153, 643, 881, 1200
HspAI GCGC 1 cut(s) 744
KflI GGGWCCC 1 cut(s) 795
KspI CCGCGG 1 cut(s) 931
Kzo9I GATC 2 cut(s) 12, 1042
LmnI GCTCC 2 cut(s) 715, 755
Lsp1109I GCAGC 2 cut(s) 1043, 1061
LweI GCATC 4 cut(s) 462, 634, 1026, 1178
MabI ACCWGGT 1 cut(s) 948
MaeI CTAG 4 cut(s) 110, 900, 962, 1125
MaeII ACGT 3 cut(s) 19, 1022, 1134
MaeIII GTNAC 2 cut(s) 880, 1010
MalI GATC 2 cut(s) 14, 1044
MboI GATC 2 cut(s) 12, 1042
MboII GAAGA 8 cut(s) 122, 125, 302, 443, 466, 469, 596, 675
MhlI GDGCHC 3 cut(s) 379, 634, 667
MlsI TGGCCA 1 cut(s) 206
MluCI AATT 4 cut(s) 254, 274, 330, 730
MluNI TGGCCA 1 cut(s) 206
MmeI TCCRAC 2 cut(s) 274, 379
MnlI CCTC 5 cut(s) 84, 249, 421, 427, 546
Mox20I TGGCCA 1 cut(s) 206
MroXI GAANNNNTTC 2 cut(s) 873, 987
MscI TGGCCA 1 cut(s) 206
MseI TTAA 2 cut(s) 852, 1137
MslI CAYNNNNRTG 1 cut(s) 537
Msp20I TGGCCA 1 cut(s) 206
MspA1I CMGCKG 1 cut(s) 930
MspI CCGG 1 cut(s) 936
MspR9I CCNGG 1 cut(s) 950
Mva1269I GAATGC 1 cut(s) 987
MvaI CCWGG 1 cut(s) 950
MvnI CGCG 2 cut(s) 746, 930
MwoI GCNNNNNNNGC 2 cut(s) 419, 520
NcoI CCATGG 1 cut(s) 149
NdeII GATC 2 cut(s) 12, 1042
NlaIII CATG 5 cut(s) 65, 153, 643, 881, 1200
NlaIV GGNNCC 3 cut(s) 796, 797, 934
NspI RCATGY 1 cut(s) 65
PaeI GCATGC 1 cut(s) 65
PagI TCATGA 1 cut(s) 1196
PceI AGGCCT 1 cut(s) 514
PctI GAATGC 1 cut(s) 987
PdmI GAANNNNTTC 2 cut(s) 873, 987
PfeI GAWTC 3 cut(s) 104, 460, 830
PflMI CCANNNNNTGG 1 cut(s) 955
PkrI GCNGC 2 cut(s) 1033, 1051
PpuMI RGGWCCY 1 cut(s) 795
PshBI ATTAAT 1 cut(s) 852
Psp5II RGGWCCY 1 cut(s) 795
Psp6I CCWGG 1 cut(s) 948
PspEI GGTNACC 1 cut(s) 1010
PspGI CCWGG 1 cut(s) 948
PspN4I GGNNCC 3 cut(s) 796, 797, 934
PspPI GGNCC 2 cut(s) 338, 795
PspPPI RGGWCCY 1 cut(s) 795
PstI CTGCAG 1 cut(s) 1033
RsaI GTAC 2 cut(s) 311, 1129
RsaNI GTAC 2 cut(s) 310, 1128
RseI CAYNNNNRTG 1 cut(s) 537
SacII CCGCGG 1 cut(s) 931
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 852, 1137
SatI GCNGC 2 cut(s) 1032, 1050
Sau3AI GATC 2 cut(s) 12, 1042
Sau96I GGNCC 2 cut(s) 338, 795
ScrFI CCNGG 1 cut(s) 950
SduI GDGCHC 3 cut(s) 379, 634, 667
SexAI ACCWGGT 1 cut(s) 948
SfaNI GCATC 4 cut(s) 462, 634, 1026, 1178
SfcI CTRYAG 1 cut(s) 1029
Sfr303I CCGCGG 1 cut(s) 931
SgrBI CCGCGG 1 cut(s) 931
SinI GGWCC 1 cut(s) 795
SmiMI CAYNNNNRTG 1 cut(s) 537
SmlI CTYRAG 1 cut(s) 1095
SmoI CTYRAG 1 cut(s) 1095
SphI GCATGC 1 cut(s) 65
Sse9I AATT 4 cut(s) 254, 274, 330, 730
SseBI AGGCCT 1 cut(s) 514
SsiI CCGC 5 cut(s) 46, 865, 928, 930, 1015
SspMI CTAG 4 cut(s) 110, 900, 962, 1125
StuI AGGCCT 1 cut(s) 514
StyD4I CCNGG 1 cut(s) 948
StyI CCWWGG 3 cut(s) 149, 423, 1070
TaaI ACNGT 3 cut(s) 43, 1184, 1192
TaiI ACGT 3 cut(s) 22, 1025, 1137
TaqI TCGA 3 cut(s) 5, 15, 994
TasI AATT 4 cut(s) 254, 274, 330, 730
TatI WGTACW 2 cut(s) 309, 1127
TfiI GAWTC 3 cut(s) 104, 460, 830
Tru1I TTAA 2 cut(s) 852, 1137
Tru9I TTAA 2 cut(s) 852, 1137
TscAI CASTG 5 cut(s) 126, 610, 658, 667, 725
TseI GCWGC 2 cut(s) 1031, 1049
TspDTI ATGAA 9 cut(s) 303, 533, 597, 675, 690, 822, 866, 980, 1104
TspRI CASTG 5 cut(s) 126, 610, 658, 667, 725
Van91I CCANNNNNTGG 1 cut(s) 955
VpaK11BI GGWCC 1 cut(s) 795
VspI ATTAAT 1 cut(s) 852
XapI RAATTY 1 cut(s) 274
XceI RCATGY 1 cut(s) 65
XmiI GTMKAC 2 cut(s) 5, 498
XmnI GAANNNNTTC 2 cut(s) 873, 987
XspI CTAG 4 cut(s) 110, 900, 962, 1125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.