Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0002179

Physical Location & Seq

Genomic Coordinates Forward (+)

4442885 .. 4448996

Gene Structure 6112 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00161760.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2040 bp

ATGCCTGAGCTGCGCCGCAGAAATCGGACCCCTAAAAACCCCGACCCGAACCCGATTACCCGAAGGGCCGCACTGAGGAAGACGAATTGTGGTAAAATTAATAAGAGTAGGAATAATCGGAAGAAGAAGAGCGGTCCGGTACTCGAAACGAGGTCGTTTGAGTTGAAGGAACACCAGGTTAGGGATTTCAGAGCCATGGATGAGTACGATAGCGGTGGCCGCAGCGGCGATAAGGGTCCGGTAGCTGAGGAAGAAATCTCAGCTCCTATTCCTGACAAGGTCCAGGTTGGTGGTTCCCCACTGTACAAAGTAGAGAGGAAATTGGGGAAAGGAGGCTTTGGACAAGTATATGTTGGCCGGCGCACTGCACCCGGCCCTGGAGCCATTGAGGTTGCCTTGAAATTTGAGCATAGAAGCAGTAAAGGATGTAATTATGGCCCACCATATGAATGGCAAATTTACAATGCACTTGGTGGTAGTCATGGAGTTCCAAGAGTTCATTATAAGGGCCGGCAAGCTGACTACTATATCATGGTTATGGATATTCTTGGGCCTAGTTTGTGGGATGTCTGGAATAACAGCTCGCACACAATGTCAATTGAAATGGTTGCATGTATTGCCATTGAGGCAATTTCCATATTGGAGAAGATGCACTCTAGAGGATATGTGCATGGAGATGTGAAACCTGAGAACTTTTTGCTTGGTCCGCCTGGAACTCCAGAGGAGAAGAAGCTATTCCTTGTTGACCTTGGATTAGCCACCAGGTGGCGAGATAGTTCAACTGGCCTGCATGTAGATTATGATCAACGTCCAGATGTTTTCAGGGGAACTGTGCGGTATGCAAGTGTGCATGCTCATCTTGGTAGAACTGGTAGCAGGAGAGATGACTTGGAATCTTTAGCTTACACATTGATATTTCTTCTACGTGGGCGGTTACCTTGGCAAGGATACCAGGGAGAAAATAAAGGATTCCTTGTCTGCAAGAAAAAGATGGCCACTTCTCCAGAAACTCTATGTTGCTTTTGCCCTCAACCATTCAGACTGTTTGTTGAGTATGTGGTGAATTTGAAGTTTGATGAAGAACCGAACTATGCAAAATATATTTCCCTTTTTGATGGAGTTGTTGGTCCAAATCCAGATATTAGACCAATAAATACTGATGGTGCTCAGAAGGTTGGACAAAAGAGAGGGCGATTAACCATGGAGGAAGAGGACGACGAACAGCCAAAGAAGAAAATTCGCATGGGGATGCCGGCAACACAATGGATTAGTGTTTACAATGCTCGTAGGCCTATGAAGCAAAGATATCACTACAATGTGGCAGATGTGAGGCTTTCCCAACATATTGAGAAAGGAAATGAAGATGGGTTGTTTATCAGCAGTGTGGCTTCCTGTTCAAATCTTTGGGCTCTCATTATGGATGCTGGAACTGGATTCAGTTCCCAAGTTTATGAACTCTCTCCATTTTTTCTCCACAAGGAATGGATCATGGAACAATGGGACAAAAATTTCTATATCAGCGCAATAGCAGGAGCTAACAATGGAAGCTCTTTGGTTGTAATGTCTAAAGGGACCTCATTCTTACAGCAGTCCTATAAAGTCAGCGAGTCATTTCCTTTCAAGTGGATTAATAAAAAATGGAGGGAAGGATTTTATGTTACTGCGATGGCCACTGCTGGGAGTAGATGGGCTATTGTTATGTCTCGGGGTGCAGGATTTTCTGACCAGGTTGTAGAACTAGATTTTCTGTATCCTAGTGAAGGTATTCATCGAAGGTGGGACAGTGGTTACCGCATCACGTCAACTGCAGCAACTTGGGATCAGGCAGCTCTCGTTCTTAGTGTTCCAAGGAGAAAACCTGCTGATGAAACACAAGAGACTCTTCGAACCTCTGCTTTTCCAAGTACACATGTGAAGCTATGTGTTTCATCATTGACTGGCTTTCGGTTTAACTGGACAATACAGCACTCAGTTCAATTTTGTATGCTAGAACTGGTATTAAGAAAAGCTGGAATGTGGCTTTGTGAGGCAAGTTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0030100 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0032879 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0060627 GO:0065007 GO:0071704 GO:0097159 GO:0097367 GO:0098657 GO:0140096 GO:1901265 GO:1901363 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

679

Amino Acids

76.91

Weight (kDa)

9.3

Isoelectric Point (pI)

50.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	102 - 329	1.6e-17	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	103 - 258	2e-06	Protein tyrosine and serine/threonine kinase
Pkinase_fungal	PF17667	201 - 306	3.3e-07	Fungal protein kinase
DUF7477	PF24289	390 - 639	3.2e-152	Domain of unknown function (DUF7477)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002179)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25760 AT2G25760
fragaria_vesca	FvH4_2g35440 FvH4_2g35440 FvH4_6g32280
malus_domestica	MD08G1111700.v1.1 MD09G1204700.v1.1 MD15G1091200.v1.1 MD17G1184900.v1.1
prunus_persica	Prupe.1G445600_v2.0.a1 Prupe.1G445600_v2.0.a1 Prupe.1G445600_v2.0.a1 Prupe.1G445600_v2.0.a1 Prupe.1G445600_v2.0.a1 Prupe.3G060400_v2.0.a1 Prupe.3G060400_v2.0.a1
pyrus_communis	pycom08g09280 pycom09g12160 pycom15g08520 pycom17g19440
rosa_chinensis	RchiOBHm_Chr2g0140231 RchiOBHm_Chr6g0306451
rosa_laevigata	RLG00000004784 RLG00000008671 RLG00000010797 RLG00000019857
rosa_multiflora	Rmu_sc0002971.1_g000017 Rmu_sc0005446.1_g000008
rosa_roxburghii	Rroxscaffold_2G00104970 Rroxscaffold_7G00161760
rosa_rugosa	Rorug02G0361500 Rorug06G0349500 Rorug06G0349600
rosa_samantha	Rh2AG412400 Rh2BG422700 Rh2CG398600 Rh6AG461700 Rh6BG439300 Rh6CG475800 Rh6DG462500
rosa_wichuraiana	Rw2G033800 Rw6G040240 Rw7G007580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	504
Acc36I	ACCTGC	1 cut(s)	1867
AccB7I	CCANNNNNTGG	1 cut(s)	765
AccBSI	CCGCTC	1 cut(s)	132
AclWI	GGATC	2 cut(s)	1493, 1827
AcoI	YGGCCR	4 cut(s)	217, 355, 993, 1668
AcsI	RAATTY	5 cut(s)	401, 456, 1063, 1236, 1507
AdeI	CACNNNGTG	2 cut(s)	473, 765
AfaI	GTAC	4 cut(s)	141, 206, 305, 1906
AfiI	CCNNNNNNNGG	7 cut(s)	75, 181, 377, 765, 944, 1677, 1760
AflIII	ACRYGT	1 cut(s)	1909
AgsI	TTSAA	8 cut(s)	166, 400, 602, 780, 1069, 1398, 1621, 1976
AjiI	CACGTC	1 cut(s)	1800
AjnI	CCWGG	7 cut(s)	174, 282, 376, 709, 761, 951, 1725
Alw21I	GWGCWC	1 cut(s)	1168
Alw26I	GTCTC	2 cut(s)	1707, 1871
AlwI	GGATC	2 cut(s)	1493, 1827
Ama87I	CYCGRG	1 cut(s)	1704
ApeKI	GCWGC	4 cut(s)	10, 222, 1808, 1826
ApoI	RAATTY	5 cut(s)	401, 456, 1063, 1236, 1507
AseI	ATTAAT	2 cut(s)	99, 1629
Asp700I	GAANNNNTTC	2 cut(s)	734, 1764
AspLEI	GCGC	3 cut(s)	15, 363, 1523
AsuC2I	CCSGG	1 cut(s)	372
AsuHPI	GGTGA	1 cut(s)	1072
AsuII	TTCGAA	1 cut(s)	1885
AvaI	CYCGRG	1 cut(s)	1704
AvaII	GGWCC	7 cut(s)	27, 134, 236, 280, 704, 1127, 1572
BalI	TGGCCA	2 cut(s)	995, 1670
BanII	GRGCYC	1 cut(s)	1411
BbsI	GAAGAC	1 cut(s)	86
Bbv12I	GWGCWC	1 cut(s)	1168
BbvCI	CCTCAGC	1 cut(s)	246
BbvI	GCAGC	3 cut(s)	234, 1820, 1838
BccI	CCATC	6 cut(s)	985, 1109, 1154, 1358, 1660, 1680
BciT130I	CCWGG	7 cut(s)	176, 284, 378, 711, 763, 953, 1727
BciVI	GTATCC	2 cut(s)	941, 1761
BclI	TGATCA	1 cut(s)	802
BcnI	CCSGG	1 cut(s)	372
BcoDI	GTCTC	2 cut(s)	1707, 1871
BfaI	CTAG	5 cut(s)	555, 657, 1739, 1755, 1988
BfmI	CTRYAG	1 cut(s)	1806
BfuAI	ACCTGC	1 cut(s)	1867
BfuI	GTATCC	2 cut(s)	941, 1761
BglI	GCCNNNNNGGC	2 cut(s)	225, 626
BisI	GCNGC	8 cut(s)	11, 16, 69, 220, 223, 226, 1809, 1827
BlsI	GCNGC	8 cut(s)	12, 17, 70, 221, 224, 227, 1810, 1828
Bme1390I	CCNGG	8 cut(s)	176, 284, 372, 378, 711, 763, 953, 1727
Bme18I	GGWCC	7 cut(s)	27, 134, 236, 280, 704, 1127, 1572
BmeT110I	CYCGRG	1 cut(s)	1704
BmgBI	CACGTC	1 cut(s)	1800
BmiI	GGNNCC	5 cut(s)	29, 237, 295, 382, 1573
BmrFI	CCNGG	8 cut(s)	176, 284, 372, 378, 711, 763, 953, 1727
BmsI	GCATC	4 cut(s)	639, 1239, 1411, 1803
BpiI	GAAGAC	1 cut(s)	86
BpmI	CTGGAG	3 cut(s)	399, 702, 987
Bpu10I	CCTNAGC	2 cut(s)	6, 246
Bpu14I	TTCGAA	1 cut(s)	1885
BpuMI	CCSGG	1 cut(s)	372
BsaAI	YACGTR	1 cut(s)	926
BsaBI	GATNNNNATC	1 cut(s)	801
BsaJI	CCNNGG	7 cut(s)	195, 376, 748, 938, 952, 1200, 1847
BsaWI	WCCGGW	2 cut(s)	136, 238
Bsc4I	CCNNNNNNNGG	7 cut(s)	75, 181, 377, 765, 944, 1677, 1760
Bse118I	RCCGGY	3 cut(s)	357, 510, 1252
Bse1I	ACTGG	6 cut(s)	787, 874, 1435, 1942, 1958, 1998
Bse8I	GATNNNNATC	1 cut(s)	801
BseBI	CCWGG	7 cut(s)	176, 284, 378, 711, 763, 953, 1727
BseDI	CCNNGG	7 cut(s)	195, 376, 748, 938, 952, 1200, 1847
BseGI	GGATG	5 cut(s)	205, 431, 571, 1254, 1426
BseJI	GATNNNNATC	1 cut(s)	801
BseLI	CCNNNNNNNGG	7 cut(s)	75, 181, 377, 765, 944, 1677, 1760
BseMII	CTCAG	6 cut(s)	65, 237, 273, 678, 1181, 1983
BseNI	ACTGG	6 cut(s)	787, 874, 1435, 1942, 1958, 1998
BseRI	GAGGAG	1 cut(s)	737
BseXI	GCAGC	3 cut(s)	234, 1820, 1838
BseYI	CCCAGC	1 cut(s)	1676
BsgI	GTGCAG	2 cut(s)	351, 1731
BsiHKAI	GWGCWC	1 cut(s)	1168
BsiHKCI	CYCGRG	1 cut(s)	1704
BsiSI	CCGG	6 cut(s)	137, 239, 358, 372, 511, 1253
BslFI	GGGAC	3 cut(s)	1514, 1585, 1793
BslI	CCNNNNNNNGG	7 cut(s)	75, 181, 377, 765, 944, 1677, 1760
BsmAI	GTCTC	2 cut(s)	1707, 1871
BsmFI	GGGAC	3 cut(s)	1514, 1585, 1793
BsoBI	CYCGRG	1 cut(s)	1704
Bsp119I	TTCGAA	1 cut(s)	1885
Bsp1286I	GDGCHC	2 cut(s)	1168, 1411
Bsp1407I	TGTACA	1 cut(s)	303
Bsp143I	GATC	3 cut(s)	802, 1485, 1819
Bsp19I	CCATGG	2 cut(s)	195, 1200
BspCNI	CTCAG	6 cut(s)	66, 238, 272, 679, 1180, 1982
BspLI	GGNNCC	5 cut(s)	29, 237, 295, 382, 1573
BspMAI	CTGCAG	1 cut(s)	1810
BspMI	ACCTGC	1 cut(s)	1867
BspPI	GGATC	2 cut(s)	1493, 1827
BspQI	GCTCTTC	1 cut(s)	122
BspT104I	TTCGAA	1 cut(s)	1885
BsrBI	CCGCTC	1 cut(s)	132
BsrFI	RCCGGY	3 cut(s)	357, 510, 1252
BsrGI	TGTACA	1 cut(s)	303
BsrI	ACTGG	6 cut(s)	787, 874, 1435, 1942, 1958, 1998
BssAI	RCCGGY	3 cut(s)	357, 510, 1252
BssECI	CCNNGG	7 cut(s)	195, 376, 748, 938, 952, 1200, 1847
BssMI	GATC	3 cut(s)	802, 1485, 1819
BssT1I	CCWWGG	5 cut(s)	195, 748, 938, 1200, 1847
Bst2UI	CCWGG	7 cut(s)	176, 284, 378, 711, 763, 953, 1727
Bst4CI	ACNGT	4 cut(s)	303, 832, 1044, 1784
Bst6I	CTCTTC	3 cut(s)	122, 1203, 1887
BstAPI	GCANNNNNTGC	1 cut(s)	617
BstAUI	TGTACA	1 cut(s)	303
BstBAI	YACGTR	1 cut(s)	926
BstBI	TTCGAA	1 cut(s)	1885
BstC8I	GCNNGC	7 cut(s)	359, 512, 516, 584, 788, 852, 1254
BstDEI	CTNAG	8 cut(s)	6, 74, 246, 259, 687, 1167, 1838, 1969
BstDSI	CCRYGG	2 cut(s)	195, 1200
BstEII	GGTNACC	2 cut(s)	933, 1787
BstENI	CCTNNNNNAGG	2 cut(s)	942, 1758
BstF5I	GGATG	5 cut(s)	205, 431, 571, 1254, 1426
BstHHI	GCGC	3 cut(s)	15, 363, 1523
BstKTI	GATC	3 cut(s)	805, 1488, 1822
BstMAI	GTCTC	2 cut(s)	1707, 1871
BstMBI	GATC	3 cut(s)	802, 1485, 1819
BstMWI	GCNNNNNNNGC	7 cut(s)	10, 219, 225, 617, 626, 706, 1297
BstNI	CCWGG	7 cut(s)	176, 284, 378, 711, 763, 953, 1727
BstNSI	RCATGY	4 cut(s)	615, 794, 854, 1913
BstPI	GGTNACC	2 cut(s)	933, 1787
BstSCI	CCNGG	8 cut(s)	174, 282, 370, 376, 709, 761, 951, 1725
BstSFI	CTRYAG	1 cut(s)	1806
BstV1I	GCAGC	3 cut(s)	234, 1820, 1838
BstV2I	GAAGAC	1 cut(s)	86
BstXI	CCANNNNNNTGG	2 cut(s)	290, 450
BsuI	GTATCC	2 cut(s)	941, 1761
BtgI	CCRYGG	2 cut(s)	195, 1200
BtgZI	GCGATG	1 cut(s)	1679
BtrI	CACGTC	1 cut(s)	1800
BtsCI	GGATG	5 cut(s)	205, 431, 571, 1254, 1426
BtsI	GCAGTG	3 cut(s)	363, 1387, 1671
BtsIMutI	CAGTG	6 cut(s)	71, 299, 363, 1387, 1671, 1789
BveI	ACCTGC	1 cut(s)	1867
Cac8I	GCNNGC	7 cut(s)	359, 512, 516, 584, 788, 852, 1254
CfoI	GCGC	3 cut(s)	15, 363, 1523
Cfr10I	RCCGGY	3 cut(s)	357, 510, 1252
CpoI	CGGWCCG	1 cut(s)	134
CsiI	ACCWGGT	3 cut(s)	174, 761, 1725
Csp6I	GTAC	4 cut(s)	140, 205, 304, 1905
CspI	CGGWCCG	1 cut(s)	134
CviQI	GTAC	4 cut(s)	140, 205, 304, 1905
DdeI	CTNAG	8 cut(s)	6, 74, 246, 259, 687, 1167, 1838, 1969
DpnI	GATC	3 cut(s)	804, 1487, 1821
DpnII	GATC	3 cut(s)	802, 1485, 1819
DraIII	CACNNNGTG	2 cut(s)	473, 765
EaeI	YGGCCR	4 cut(s)	217, 355, 993, 1668
Eam1104I	CTCTTC	3 cut(s)	122, 1203, 1887
EarI	CTCTTC	3 cut(s)	122, 1203, 1887
EciI	GGCGGA	1 cut(s)	696
Eco130I	CCWWGG	5 cut(s)	195, 748, 938, 1200, 1847
Eco147I	AGGCCT	1 cut(s)	1291
Eco24I	GRGCYC	1 cut(s)	1411
Eco32I	GATATC	1 cut(s)	1307
Eco47I	GGWCC	7 cut(s)	27, 134, 236, 280, 704, 1127, 1572
Eco88I	CYCGRG	1 cut(s)	1704
Eco91I	GGTNACC	2 cut(s)	933, 1787
EcoNI	CCTNNNNNAGG	2 cut(s)	942, 1758
EcoO109I	RGGNCCY	1 cut(s)	1572
EcoO65I	GGTNACC	2 cut(s)	933, 1787
EcoRII	CCWGG	7 cut(s)	174, 282, 376, 709, 761, 951, 1725
EcoRV	GATATC	1 cut(s)	1307
EcoT14I	CCWWGG	5 cut(s)	195, 748, 938, 1200, 1847
EcoT38I	GRGCYC	1 cut(s)	1411
ErhI	CCWWGG	5 cut(s)	195, 748, 938, 1200, 1847
FalI	AAGNNNNNCTT	4 cut(s)	957, 989, 1866, 1898
FaqI	GGGAC	3 cut(s)	1514, 1585, 1793
FauNDI	CATATG	1 cut(s)	445
FbaI	TGATCA	1 cut(s)	802
Fnu4HI	GCNGC	8 cut(s)	11, 16, 69, 220, 223, 226, 1809, 1827
FokI	GGATG	5 cut(s)	212, 438, 578, 1261, 1433
FriOI	GRGCYC	1 cut(s)	1411
Fsp4HI	GCNGC	8 cut(s)	11, 16, 69, 220, 223, 226, 1809, 1827
FspBI	CTAG	5 cut(s)	555, 657, 1739, 1755, 1988
GlaI	GCGC	3 cut(s)	14, 362, 1522
GluI	GCNGC	8 cut(s)	11, 16, 69, 220, 223, 226, 1809, 1827
GsaI	CCCAGC	1 cut(s)	1680
GsuI	CTGGAG	3 cut(s)	399, 702, 987
HapII	CCGG	6 cut(s)	137, 239, 358, 372, 511, 1253
HhaI	GCGC	3 cut(s)	15, 363, 1523
Hin6I	GCGC	3 cut(s)	13, 361, 1521
HinP1I	GCGC	3 cut(s)	13, 361, 1521
HincII	GTYRAC	2 cut(s)	745, 1803
HindII	GTYRAC	2 cut(s)	745, 1803
HinfI	GANTC	5 cut(s)	893, 969, 1434, 1607, 1879
HpaII	CCGG	6 cut(s)	137, 239, 358, 372, 511, 1253
HphI	GGTGA	1 cut(s)	1072
Hpy166II	GTNNAC	4 cut(s)	745, 1276, 1803, 1907
Hpy188I	TCNGA	6 cut(s)	27, 120, 191, 1040, 1170, 1723
Hpy188III	TCNNGA	7 cut(s)	272, 571, 657, 719, 812, 1004, 1136
Hpy8I	GTNNAC	4 cut(s)	745, 1276, 1803, 1907
Hpy99I	CGWCG	1 cut(s)	1220
HpyAV	CCTTC	6 cut(s)	57, 160, 1165, 1640, 1754, 1767
HpyCH4III	ACNGT	4 cut(s)	303, 832, 1044, 1784
HpyCH4IV	ACGT	3 cut(s)	808, 925, 1799
HpyF10VI	GCNNNNNNNGC	7 cut(s)	10, 219, 225, 617, 626, 706, 1297
HpyF3I	CTNAG	8 cut(s)	6, 74, 246, 259, 687, 1167, 1838, 1969
HpySE526I	ACGT	3 cut(s)	808, 925, 1799
HspAI	GCGC	3 cut(s)	13, 361, 1521
KroI	GCCGGC	3 cut(s)	357, 510, 1252
KroNI	GCCGGC	3 cut(s)	359, 512, 1254
Ksp22I	TGATCA	1 cut(s)	802
Kzo9I	GATC	3 cut(s)	802, 1485, 1819
LguI	GCTCTTC	1 cut(s)	122
LmnI	GCTCC	3 cut(s)	268, 380, 1532
Lsp1109I	GCAGC	3 cut(s)	234, 1820, 1838
LweI	GCATC	4 cut(s)	639, 1239, 1411, 1803
MabI	ACCWGGT	3 cut(s)	174, 761, 1725
MaeI	CTAG	5 cut(s)	555, 657, 1739, 1755, 1988
MaeII	ACGT	3 cut(s)	808, 925, 1799
MaeIII	GTNAC	3 cut(s)	933, 1657, 1787
MalI	GATC	3 cut(s)	804, 1487, 1821
MbiI	CCGCTC	1 cut(s)	132
MboI	GATC	3 cut(s)	802, 1485, 1819
MfeI	CAATTG	1 cut(s)	597
MhlI	GDGCHC	2 cut(s)	1168, 1411
MlsI	TGGCCA	2 cut(s)	995, 1670
MluNI	TGGCCA	2 cut(s)	995, 1670
MlyI	GAGTC	2 cut(s)	1616, 1873
MmeI	TCCRAC	1 cut(s)	1156
Mox20I	TGGCCA	2 cut(s)	995, 1670
MroNI	GCCGGC	3 cut(s)	357, 510, 1252
MroXI	GAANNNNTTC	2 cut(s)	734, 1764
MscI	TGGCCA	2 cut(s)	995, 1670
MseI	TTAA	5 cut(s)	99, 1196, 1629, 1950, 2000
MslI	CAYNNNNRTG	5 cut(s)	448, 536, 675, 1247, 1314
Msp20I	TGGCCA	2 cut(s)	995, 1670
MspA1I	CMGCKG	1 cut(s)	225
MspI	CCGG	6 cut(s)	137, 239, 358, 372, 511, 1253
MspR9I	CCNGG	8 cut(s)	176, 284, 372, 378, 711, 763, 953, 1727
MunI	CAATTG	1 cut(s)	597
MvaI	CCWGG	7 cut(s)	176, 284, 378, 711, 763, 953, 1727
MwoI	GCNNNNNNNGC	7 cut(s)	10, 219, 225, 617, 626, 706, 1297
NaeI	GCCGGC	3 cut(s)	359, 512, 1254
NciI	CCSGG	1 cut(s)	372
NcoI	CCATGG	2 cut(s)	195, 1200
NdeI	CATATG	1 cut(s)	445
NdeII	GATC	3 cut(s)	802, 1485, 1819
NgoMIV	GCCGGC	3 cut(s)	357, 510, 1252
NlaIV	GGNNCC	5 cut(s)	29, 237, 295, 382, 1573
NspI	RCATGY	4 cut(s)	615, 794, 854, 1913
NspV	TTCGAA	1 cut(s)	1885
PaeI	GCATGC	1 cut(s)	854
PceI	AGGCCT	1 cut(s)	1291
PciI	ACATGT	1 cut(s)	1909
PciSI	GCTCTTC	1 cut(s)	122
PdiI	GCCGGC	3 cut(s)	359, 512, 1254
PdmI	GAANNNNTTC	2 cut(s)	734, 1764
PfeI	GAWTC	3 cut(s)	893, 969, 1434
PflFI	GACNNNGTC	1 cut(s)	278
PflMI	CCANNNNNTGG	1 cut(s)	765
PkrI	GCNGC	8 cut(s)	12, 17, 70, 221, 224, 227, 1810, 1828
PleI	GAGTC	2 cut(s)	1615, 1873
PpsI	GAGTC	2 cut(s)	1615, 1873
Ppu21I	YACGTR	1 cut(s)	926
PpuMI	RGGWCCY	1 cut(s)	1572
PscI	ACATGT	1 cut(s)	1909
PshBI	ATTAAT	2 cut(s)	99, 1629
PsiI	TTATAA	1 cut(s)	504
Psp5II	RGGWCCY	1 cut(s)	1572
Psp6I	CCWGG	7 cut(s)	174, 282, 376, 709, 761, 951, 1725
PspEI	GGTNACC	2 cut(s)	933, 1787
PspFI	CCCAGC	1 cut(s)	1676
PspGI	CCWGG	7 cut(s)	174, 282, 376, 709, 761, 951, 1725
PspN4I	GGNNCC	5 cut(s)	29, 237, 295, 382, 1573
PspPPI	RGGWCCY	1 cut(s)	1572
PstI	CTGCAG	1 cut(s)	1810
PsyI	GACNNNGTC	1 cut(s)	278
RsaI	GTAC	4 cut(s)	141, 206, 305, 1906
RsaNI	GTAC	4 cut(s)	140, 205, 304, 1905
RseI	CAYNNNNRTG	5 cut(s)	448, 536, 675, 1247, 1314
Rsr2I	CGGWCCG	1 cut(s)	134
RsrII	CGGWCCG	1 cut(s)	134
SapI	GCTCTTC	1 cut(s)	122
SaqAI	TTAA	5 cut(s)	99, 1196, 1629, 1950, 2000
SatI	GCNGC	8 cut(s)	11, 16, 69, 220, 223, 226, 1809, 1827
Sau3AI	GATC	3 cut(s)	802, 1485, 1819
SchI	GAGTC	2 cut(s)	1616, 1873
ScrFI	CCNGG	8 cut(s)	176, 284, 372, 378, 711, 763, 953, 1727
SduI	GDGCHC	2 cut(s)	1168, 1411
SexAI	ACCWGGT	3 cut(s)	174, 761, 1725
SfaNI	GCATC	4 cut(s)	639, 1239, 1411, 1803
SfcI	CTRYAG	1 cut(s)	1806
SfuI	TTCGAA	1 cut(s)	1885
SinI	GGWCC	7 cut(s)	27, 134, 236, 280, 704, 1127, 1572
SmiMI	CAYNNNNRTG	5 cut(s)	448, 536, 675, 1247, 1314
SphI	GCATGC	1 cut(s)	854
SseBI	AGGCCT	1 cut(s)	1291
SspMI	CTAG	5 cut(s)	555, 657, 1739, 1755, 1988
StuI	AGGCCT	1 cut(s)	1291
StyD4I	CCNGG	8 cut(s)	174, 282, 370, 376, 709, 761, 951, 1725
StyI	CCWWGG	5 cut(s)	195, 748, 938, 1200, 1847
TaaI	ACNGT	4 cut(s)	303, 832, 1044, 1784
TaiI	ACGT	3 cut(s)	811, 928, 1802
TaqI	TCGA	3 cut(s)	144, 1771, 1885
TatI	WGTACW	2 cut(s)	303, 1904
TauI	GCSGC	4 cut(s)	18, 71, 222, 228
TfiI	GAWTC	3 cut(s)	893, 969, 1434
Tru1I	TTAA	5 cut(s)	99, 1196, 1629, 1950, 2000
Tru9I	TTAA	5 cut(s)	99, 1196, 1629, 1950, 2000
TscAI	CASTG	6 cut(s)	78, 306, 370, 1387, 1678, 1789
TseI	GCWGC	4 cut(s)	10, 222, 1808, 1826
TspDTI	ATGAA	9 cut(s)	462, 488, 1092, 1310, 1374, 1467, 1757, 1881, 1917
TspRI	CASTG	6 cut(s)	78, 306, 370, 1387, 1678, 1789
Tth111I	GACNNNGTC	1 cut(s)	278
Van91I	CCANNNNNTGG	1 cut(s)	765
VpaK11BI	GGWCC	7 cut(s)	27, 134, 236, 280, 704, 1127, 1572
VspI	ATTAAT	2 cut(s)	99, 1629
XagI	CCTNNNNNAGG	2 cut(s)	942, 1758
XapI	RAATTY	5 cut(s)	401, 456, 1063, 1236, 1507
XbaI	TCTAGA	1 cut(s)	656
XceI	RCATGY	4 cut(s)	615, 794, 854, 1913
XcmI	CCANNNNNNNNNTGG	1 cut(s)	447
XmnI	GAANNNNTTC	2 cut(s)	734, 1764
XspI	CTAG	5 cut(s)	555, 657, 1739, 1755, 1988

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.