MD15G1102100.v1.1

domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002777
Physical Location & Seq
Forward (+)
7230266 .. 7233535
3270 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1102100.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGAGGGTATGTGCCTACAAAGACGGGGAAGTTATTATTGACACTGCTGCTGGAGTGCTTGGTAGATATGATCCTCGTCCAGTTCAGCCCGACAGCCTTTTCCCAGTTTTTTCTGCTACAAAGGGTATAACGGCTGGAATGTTACATTGGCTGGCAGACACTGGAAAATTGAACCTCAAGGAAGATGTAGCAAATATATGGCCAGAATTTGGATCATTTGGAAAACATAACATAAAGGTCCATCATGTCCTTAACCATACCTCTGGACTGCACAATGCTGCGGCAGACCTCATAGAAAACCCTTTGCTTATCTCAGACTGGGAGGAGTGTTTAAACCGCGTTGCCCTTTCAGAACCTGAGACTGAACCTGGCCAGGAGCAGCTGTATCACTACCTAACTTTTGGTTGGATATGCGGTGGAATCATTGAGCATGCATGGAAGAGGAAATTGAAGGAGATTCTTGAAGAAGCATTTGTTCGTTCCCTCCAAATAGAAGGGGAGTTTTATATCGGAATACCTCCAGGTGTGGAATCTCGACTTGCAACCCTTACACCCGATACAGAAGACCTGAAGAAGCTTTCAGGGATTAGGGGTCGTCCTGATATGCCCTCCACTTTCCAGCCAGAGCAATTCGCAACTACGTTGCCCGTTCTATTTATCATGCTAAACGTTCGTCGTGCCATGATACCTGCAGCTAATGGACATTTCTCAGCCCGTGCGCTTGCACGTTACTATGCAGCCTTAGCTGATGGCGGTGTTGTTCCACCGCCTCACTTCTCTTCCTCTAAGCCATCTCATGGTAGCCACCCTCACATTCCCAAATTTCCTGCCCAACCGTCACCAAAACAGCAGAAAGGTAACAGAAGCAAGAAGATAGCTGCTGCTTTTAGGAATATGAGAACTAGTAAGTATGAGAAGACTCCACAAGATAGCAAGGACCAGGACGTTGGTAGTCACAGTAGAAACACAAGCAGCGACAGTAACACTTGCAGCGCAAGTGACAACGTGTTGGATGAGATCGTTGTGAATCCTAATCCTCAAAAGGACGGCGCTAAGATATTTAGCAACCCCAGAATTCATGATGCTTTCATGGGTGTCGGCGAATATGCACACTTGGTTAAACCAGATGGGAAATTTGGACTGGGATTTAAGAGGTATTATTCCCAGGACGGGTCCCTTATTGGCTTTGGCCACTCAGGAATGGGTGGCTCAACTGCTTTCTGTGACATAAAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1 Beta-lactamase WaaY

Protein Analysis

413

Amino Acids

45.33

Weight (kDa)

7.74

Isoelectric Point (pI)

47.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-lactamase PF00144 2 - 258 3.1e-37 Beta-lactamase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 697
AccB7I CCANNNNNTGG 2 cut(s) 209, 797
AccII CGCG 1 cut(s) 339
AciI CCGC 5 cut(s) 281, 337, 414, 753, 767
AclI AACGTT 1 cut(s) 669
AclWI GGATC 2 cut(s) 65, 220
AcoI YGGCCR 3 cut(s) 200, 370, 1189
AcsI RAATTY 4 cut(s) 206, 821, 1074, 1133
AcuI CTGAAG 1 cut(s) 590
AfiI CCNNNNNNNGG 3 cut(s) 209, 797, 1170
AflIII ACRYGT 1 cut(s) 1005
AgsI TTSAA 3 cut(s) 172, 451, 464
AhlI ACTAGT 1 cut(s) 902
AjnI CCWGG 5 cut(s) 367, 372, 520, 939, 1164
AjuI GAANNNNNNNTTGG 2 cut(s) 130, 162
AluBI AGCT 5 cut(s) 382, 577, 695, 746, 878
AluI AGCT 5 cut(s) 382, 577, 695, 746, 878
Alw26I GTCTC 1 cut(s) 353
AlwI GGATC 2 cut(s) 65, 220
AlwNI CAGNNNCTG 2 cut(s) 161, 356
AoxI GGCC 3 cut(s) 200, 370, 1189
ApeKI GCWGC 9 cut(s) 47, 278, 379, 692, 737, 878, 881, 972, 990
ApoI RAATTY 4 cut(s) 206, 821, 1074, 1133
AspLEI GCGC 3 cut(s) 721, 995, 1052
AspS9I GGNCC 3 cut(s) 238, 937, 1173
AsuHPI GGTGA 1 cut(s) 831
AvaII GGWCC 3 cut(s) 238, 937, 1173
BalI TGGCCA 3 cut(s) 202, 372, 1191
BbsI GAAGAC 2 cut(s) 570, 923
BbvI GCAGC 9 cut(s) 34, 265, 391, 704, 749, 865, 868, 984, 1002
BccI CCATC 4 cut(s) 249, 743, 799, 1121
BceAI ACGGC 2 cut(s) 147, 1063
BciT130I CCWGG 5 cut(s) 369, 374, 522, 941, 1166
BcoDI GTCTC 1 cut(s) 353
BcuI ACTAGT 1 cut(s) 902
BfaI CTAG 1 cut(s) 903
BfmI CTRYAG 1 cut(s) 690
BfoI RGCGCY 1 cut(s) 1053
BfuAI ACCTGC 1 cut(s) 697
Bme1390I CCNGG 5 cut(s) 369, 374, 522, 941, 1166
Bme18I GGWCC 3 cut(s) 238, 937, 1173
BmgT120I GGNCC 3 cut(s) 238, 937, 1173
BmiI GGNNCC 2 cut(s) 1174, 1175
BmrFI CCNGG 5 cut(s) 369, 374, 522, 941, 1166
BmrI ACTGGG 3 cut(s) 98, 328, 1151
BmsI GCATC 1 cut(s) 1072
BmuI ACTGGG 3 cut(s) 98, 328, 1151
BpiI GAAGAC 2 cut(s) 570, 923
BpmI CTGGAG 2 cut(s) 72, 504
Bpu10I CCTNAGC 1 cut(s) 742
BpuEI CTTGAG 1 cut(s) 161
BsaJI CCNNGG 1 cut(s) 1164
BsaXI ACNNNNNCTCC 2 cut(s) 368, 398
Bsc4I CCNNNNNNNGG 3 cut(s) 209, 797, 1170
Bse1I ACTGG 5 cut(s) 80, 104, 166, 323, 1146
BseBI CCWGG 5 cut(s) 369, 374, 522, 941, 1166
BseDI CCNNGG 1 cut(s) 1164
BseGI GGATG 1 cut(s) 1018
BseLI CCNNNNNNNGG 3 cut(s) 209, 797, 1170
BseMII CTCAG 4 cut(s) 327, 348, 723, 1209
BseNI ACTGG 5 cut(s) 80, 104, 166, 323, 1146
BseRI GAGGAG 1 cut(s) 338
BseXI GCAGC 9 cut(s) 34, 265, 391, 704, 749, 865, 868, 984, 1002
BsgI GTGCAG 1 cut(s) 254
Bsh1236I CGCG 1 cut(s) 339
BshFI GGCC 3 cut(s) 202, 372, 1191
BslFI GGGAC 1 cut(s) 1159
BslI CCNNNNNNNGG 3 cut(s) 209, 797, 1170
BsmAI GTCTC 1 cut(s) 353
BsmFI GGGAC 1 cut(s) 1159
BsnI GGCC 3 cut(s) 202, 372, 1191
Bsp143I GATC 3 cut(s) 70, 212, 1017
BspACI CCGC 5 cut(s) 281, 337, 414, 753, 767
BspANI GGCC 3 cut(s) 202, 372, 1191
BspCNI CTCAG 4 cut(s) 326, 349, 722, 1208
BspFNI CGCG 1 cut(s) 339
BspHI TCATGA 1 cut(s) 1078
BspLI GGNNCC 2 cut(s) 1174, 1175
BspMAI CTGCAG 1 cut(s) 694
BspMI ACCTGC 1 cut(s) 697
BspPI GGATC 2 cut(s) 65, 220
BsrI ACTGG 5 cut(s) 80, 104, 166, 323, 1146
BssECI CCNNGG 1 cut(s) 1164
BssMI GATC 3 cut(s) 70, 212, 1017
Bst2UI CCWGG 5 cut(s) 369, 374, 522, 941, 1166
Bst4CI ACNGT 3 cut(s) 837, 959, 980
Bst6I CTCTTC 2 cut(s) 434, 784
BstC8I GCNNGC 3 cut(s) 153, 432, 723
BstDEI CTNAG 7 cut(s) 313, 357, 709, 742, 786, 1053, 1195
BstF5I GGATG 1 cut(s) 1018
BstFNI CGCG 1 cut(s) 339
BstH2I RGCGCY 1 cut(s) 1053
BstHHI GCGC 3 cut(s) 721, 995, 1052
BstKTI GATC 3 cut(s) 73, 215, 1020
BstMAI GTCTC 1 cut(s) 353
BstMBI GATC 3 cut(s) 70, 212, 1017
BstMWI GCNNNNNNNGC 1 cut(s) 743
BstNI CCWGG 5 cut(s) 369, 374, 522, 941, 1166
BstNSI RCATGY 1 cut(s) 434
BstSCI CCNGG 5 cut(s) 367, 372, 520, 939, 1164
BstSFI CTRYAG 1 cut(s) 690
BstUI CGCG 1 cut(s) 339
BstV1I GCAGC 9 cut(s) 34, 265, 391, 704, 749, 865, 868, 984, 1002
BstV2I GAAGAC 2 cut(s) 570, 923
BstXI CCANNNNNNTGG 2 cut(s) 263, 947
BsuRI GGCC 3 cut(s) 202, 372, 1191
BtsCI GGATG 1 cut(s) 1018
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 2 cut(s) 42, 159
BveI ACCTGC 1 cut(s) 697
Cac8I GCNNGC 3 cut(s) 153, 432, 723
CaiI CAGNNNCTG 2 cut(s) 161, 356
CciI TCATGA 1 cut(s) 1078
CfoI GCGC 3 cut(s) 721, 995, 1052
Cfr13I GGNCC 3 cut(s) 238, 937, 1173
CviAII CATG 8 cut(s) 245, 431, 435, 661, 682, 797, 1079, 1090
DdeI CTNAG 7 cut(s) 313, 357, 709, 742, 786, 1053, 1195
DpnI GATC 3 cut(s) 72, 214, 1019
DpnII GATC 3 cut(s) 70, 212, 1017
DraI TTTAAA 1 cut(s) 333
EaeI YGGCCR 3 cut(s) 200, 370, 1189
Eam1104I CTCTTC 2 cut(s) 434, 784
EarI CTCTTC 2 cut(s) 434, 784
Eco47I GGWCC 3 cut(s) 238, 937, 1173
Eco57I CTGAAG 1 cut(s) 590
EcoO109I RGGNCCY 1 cut(s) 1173
EcoRI GAATTC 1 cut(s) 1074
EcoRII CCWGG 5 cut(s) 367, 372, 520, 939, 1164
EcoT22I ATGCAT 1 cut(s) 436
FaeI CATG 8 cut(s) 248, 434, 438, 664, 685, 800, 1082, 1093
FaqI GGGAC 1 cut(s) 1159
FatI CATG 8 cut(s) 244, 430, 434, 660, 681, 796, 1078, 1089
FokI GGATG 1 cut(s) 1025
FspBI CTAG 1 cut(s) 903
GlaI GCGC 3 cut(s) 720, 994, 1051
GsuI CTGGAG 2 cut(s) 72, 504
HaeII RGCGCY 1 cut(s) 1053
HaeIII GGCC 3 cut(s) 202, 372, 1191
HhaI GCGC 3 cut(s) 721, 995, 1052
Hin1II CATG 8 cut(s) 248, 434, 438, 664, 685, 800, 1082, 1093
Hin6I GCGC 3 cut(s) 719, 993, 1050
HinP1I GCGC 3 cut(s) 719, 993, 1050
HindIII AAGCTT 1 cut(s) 575
HinfI GANTC 5 cut(s) 420, 457, 530, 919, 1027
HphI GGTGA 1 cut(s) 831
Hpy188I TCNGA 3 cut(s) 316, 352, 512
Hpy188III TCNNGA 6 cut(s) 264, 461, 534, 599, 1079, 1197
Hpy99I CGWCG 1 cut(s) 678
HpyAV CCTTC 2 cut(s) 445, 488
HpyCH4III ACNGT 3 cut(s) 837, 959, 980
HpyCH4IV ACGT 5 cut(s) 641, 669, 727, 945, 1005
HpyCH4V TGCA 8 cut(s) 271, 434, 542, 692, 725, 737, 990, 1109
HpyF10VI GCNNNNNNNGC 1 cut(s) 743
HpyF3I CTNAG 7 cut(s) 313, 357, 709, 742, 786, 1053, 1195
HpySE526I ACGT 5 cut(s) 641, 669, 727, 945, 1005
Hsp92II CATG 8 cut(s) 248, 434, 438, 664, 685, 800, 1082, 1093
HspAI GCGC 3 cut(s) 719, 993, 1050
KflI GGGWCCC 1 cut(s) 1173
Kzo9I GATC 3 cut(s) 70, 212, 1017
LmnI GCTCC 1 cut(s) 376
Lsp1109I GCAGC 9 cut(s) 34, 265, 391, 704, 749, 865, 868, 984, 1002
LweI GCATC 1 cut(s) 1072
MaeI CTAG 1 cut(s) 903
MaeII ACGT 5 cut(s) 641, 669, 727, 945, 1005
MaeIII GTNAC 8 cut(s) 141, 728, 837, 857, 953, 980, 998, 1223
MalI GATC 3 cut(s) 72, 214, 1019
MboI GATC 3 cut(s) 70, 212, 1017
MboII GAAGA 8 cut(s) 194, 451, 476, 575, 583, 771, 883, 928
MlsI TGGCCA 3 cut(s) 202, 372, 1191
MluCI AATT 7 cut(s) 167, 206, 446, 629, 821, 1074, 1133
MluNI TGGCCA 3 cut(s) 202, 372, 1191
MlyI GAGTC 1 cut(s) 913
MmeI TCCRAC 2 cut(s) 386, 990
Mox20I TGGCCA 3 cut(s) 202, 372, 1191
Mph1103I ATGCAT 1 cut(s) 436
MscI TGGCCA 3 cut(s) 202, 372, 1191
MseI TTAA 4 cut(s) 252, 332, 1119, 1149
Msp20I TGGCCA 3 cut(s) 202, 372, 1191
MspA1I CMGCKG 1 cut(s) 382
MspR9I CCNGG 5 cut(s) 369, 374, 522, 941, 1166
MssI GTTTAAAC 1 cut(s) 333
MvaI CCWGG 5 cut(s) 369, 374, 522, 941, 1166
MvnI CGCG 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 743
NdeII GATC 3 cut(s) 70, 212, 1017
NlaIII CATG 8 cut(s) 248, 434, 438, 664, 685, 800, 1082, 1093
NlaIV GGNNCC 2 cut(s) 1174, 1175
NmuCI GTSAC 4 cut(s) 837, 953, 998, 1223
NsiI ATGCAT 1 cut(s) 436
NspI RCATGY 1 cut(s) 434
PaeI GCATGC 1 cut(s) 434
PagI TCATGA 1 cut(s) 1078
PfeI GAWTC 4 cut(s) 420, 457, 530, 1027
PflMI CCANNNNNTGG 2 cut(s) 209, 797
PleI GAGTC 1 cut(s) 913
PmeI GTTTAAAC 1 cut(s) 333
PpsI GAGTC 1 cut(s) 913
PpuMI RGGWCCY 1 cut(s) 1173
Psp1406I AACGTT 1 cut(s) 669
Psp5II RGGWCCY 1 cut(s) 1173
Psp6I CCWGG 5 cut(s) 367, 372, 520, 939, 1164
PspGI CCWGG 5 cut(s) 367, 372, 520, 939, 1164
PspN4I GGNNCC 2 cut(s) 1174, 1175
PspPI GGNCC 3 cut(s) 238, 937, 1173
PspPPI RGGWCCY 1 cut(s) 1173
PstI CTGCAG 1 cut(s) 694
PstNI CAGNNNCTG 2 cut(s) 161, 356
PvuII CAGCTG 1 cut(s) 382
SaqAI TTAA 4 cut(s) 252, 332, 1119, 1149
Sau3AI GATC 3 cut(s) 70, 212, 1017
Sau96I GGNCC 3 cut(s) 238, 937, 1173
SchI GAGTC 1 cut(s) 913
ScrFI CCNGG 5 cut(s) 369, 374, 522, 941, 1166
SfaNI GCATC 1 cut(s) 1072
SfcI CTRYAG 1 cut(s) 690
SinI GGWCC 3 cut(s) 238, 937, 1173
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
SpeI ACTAGT 1 cut(s) 902
SphI GCATGC 1 cut(s) 434
Sse9I AATT 7 cut(s) 167, 206, 446, 629, 821, 1074, 1133
SsiI CCGC 5 cut(s) 281, 337, 414, 753, 767
SspMI CTAG 1 cut(s) 903
StyD4I CCNGG 5 cut(s) 367, 372, 520, 939, 1164
TaaI ACNGT 3 cut(s) 837, 959, 980
TaiI ACGT 5 cut(s) 644, 672, 730, 948, 1008
TaqI TCGA 1 cut(s) 535
TasI AATT 7 cut(s) 167, 206, 446, 629, 821, 1074, 1133
TauI GCSGC 1 cut(s) 284
TfiI GAWTC 4 cut(s) 420, 457, 530, 1027
Tru1I TTAA 4 cut(s) 252, 332, 1119, 1149
Tru9I TTAA 4 cut(s) 252, 332, 1119, 1149
TscAI CASTG 2 cut(s) 49, 166
TseFI GTSAC 4 cut(s) 837, 953, 998, 1223
TseI GCWGC 9 cut(s) 47, 278, 379, 692, 737, 878, 881, 972, 990
Tsp45I GTSAC 4 cut(s) 837, 953, 998, 1223
TspDTI ATGAA 2 cut(s) 1067, 1078
TspRI CASTG 2 cut(s) 49, 166
Van91I CCANNNNNTGG 2 cut(s) 209, 797
VpaK11BI GGWCC 3 cut(s) 238, 937, 1173
XapI RAATTY 4 cut(s) 206, 821, 1074, 1133
XceI RCATGY 1 cut(s) 434
XspI CTAG 1 cut(s) 903
Zsp2I ATGCAT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.