MD15G1103400.v1.1

transcriptional co-repressor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008825
Physical Location & Seq
Reverse (-)
7294069 .. 7296915
2847 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1103400.v1.1.491

Sequence Viewer

Length: 1008 bp
ATGCAGGCTGATATGGATCGATTTGTACAGGATGGGTCTGTTGGTGATAATGTCGAGTCTTTTTTATCCCCTGATGATGTGGATCGTAGAGATGCTGTTGGTCGATGTATGGATGTCAGCAAAGGGTTCACATTTACGGAAGTAAACTCTATTAAAGCAAGCGCAAGCAAGGTTACAAGCTGTCACTTCTCATCAGATGGGAAGTTTCTTGCTAGTGGGGGCCATGATAAAAGGGCTGTATTGTGGTACACAGATACATTAAAGCTAAAATGTACGCTACAAGAACATTCAGCTTTGATAACTGATGTTCGTTTTAGTCCGAGCAAGCCATGTCTTGCAACGTCTTCTTTCGACAAGACTATCAGGGTTTGGGATGCCGACAATCCTAGTTATTCTCTCCGTACCTTTATGGGACATTCGGCCTCTGTTATGTCATTAGATTTCCACCCAAATAAAGACGACCTCATCTGTTCTTGTGACGGGGATGGTCAGATACGCTACTGGAGTATTAACAATGGCTGCTGCTCGTGCGTGTCTAAGGGTGGTACGGCACAAATAAGATTTCAACCCCGTCTTGGAAAATTTCTTGCTGCAGCCGCAGAGAATGTTGTATCTATACTGGATGTTGAGACTCAGACTTGTCAGTATTCTTTACAGGGGCATACTAAGCCTATCCATTCTGTGTGTTGGGATCCTTCTGGCGAATTCCTTGCATCTGTCAGCGAGGACTCTGTAAGAGTTTGGACACTTGGAGCAGGAAGCGAAGTGGAATGCGTTCATGAGTTTAGCTGCATCGGAAATAAATTTCATTCATGCGTTTTCCATCCTACATATACTTCACTGCTGGTCATTGGCTGCTATCAGACACTGGAGTTATGGAACATGACAGAGAACAAGACAATGACTCTACCAGCACATGAAGGACTCATTGATTCTTTGGCGGTGTCAACTGTTACAGGTTTGATTTCTTCGGCTAGTCACGATACGTTTGTCAAGATCTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

336

Amino Acids

36.79

Weight (kDa)

5.6

Isoelectric Point (pI)

34.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 37 - 109 9e-08 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 41 - 184 1.7e-11 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 43 - 116 3e-10 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 43 - 169 1.6e-14 WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 44 - 250 6.7e-15 MABP1/WDR62 second WD40 domain
WDR55 PF24796 46 - 179 2.8e-08 WDR55
Beta-prop_WDR36-Utp21_1st PF25171 47 - 125 3.9e-06 WDR36/Utp21 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 47 - 129 3e-08 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 48 - 82 1.2e-06 WD domain, G-beta repeat
EIF3I PF24805 48 - 128 1.2e-06 EIF3I
Beta-prop_WDR3_1st PF25173 51 - 170 5e-23 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 52 - 176 9.9e-27 WDR5 beta-propeller domain
WD40_Prp19 PF24814 53 - 255 2.7e-32 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 54 - 154 5.4e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 54 - 173 5e-17 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 56 - 128 1.3e-10 WDHD1 first WD40 domain
Beta-prop_EML PF23409 82 - 174 3.9e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_TEP1_2nd PF25047 85 - 172 2.6e-06 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 87 - 175 4e-13 CDC20/Fizzy WD40 domain
WD40 PF00400 88 - 125 3.5e-09 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 96 - 335 1.3e-35 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 98 - 176 6.3e-12 WDHD1 first WD40 domain
WD40 PF00400 132 - 169 8e-07 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 135 - 249 6.4e-09 WDR3 second beta-propeller domain
WDR55 PF24796 140 - 301 3.1e-07 WDR55
Beta-prop_Aladin PF25460 141 - 251 3.7e-07 Aladin seven-bladed propeller
Beta-prop_WDR3_1st PF25173 158 - 294 3.8e-14 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 162 - 260 5e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 182 - 334 2.1e-24 WDR5 beta-propeller domain
WD40_Gbeta PF25391 185 - 334 8.5e-10 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 186 - 264 3.4e-12 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 192 - 317 2.6e-07 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 196 - 317 3.5e-08 CDC20/Fizzy WD40 domain
WD40 PF00400 213 - 248 1.6e-08 WD domain, G-beta repeat
Beta-prop_EIPR1 PF23609 215 - 334 2.8e-06 EIPR1 beta-propeller
Beta-prop_WDR3_2nd PF25172 236 - 335 1e-05 WDR3 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 597, 941
AclWI GGATC 4 cut(s) 24, 90, 686, 699
AcsI RAATTY 3 cut(s) 581, 704, 803
AfaI GTAC 5 cut(s) 27, 248, 274, 403, 547
AfiI CCNNNNNNNGG 1 cut(s) 575
AgsI TTSAA 1 cut(s) 566
AluBI AGCT 4 cut(s) 180, 265, 293, 789
AluI AGCT 4 cut(s) 180, 265, 293, 789
Alw26I GTCTC 1 cut(s) 623
AlwI GGATC 4 cut(s) 24, 90, 686, 699
AlwNI CAGNNNCTG 1 cut(s) 868
AoxI GGCC 2 cut(s) 220, 420
ApeKI GCWGC 6 cut(s) 519, 522, 590, 593, 789, 855
ApoI RAATTY 3 cut(s) 581, 704, 803
Asp700I GAANNNNTTC 1 cut(s) 774
AspLEI GCGC 1 cut(s) 164
AspS9I GGNCC 1 cut(s) 220
AsuHPI GGTGA 1 cut(s) 56
BaeI ACNNNNGTAYC 2 cut(s) 975, 1008
BamHI GGATCC 1 cut(s) 691
BauI CACGAG 1 cut(s) 526
BbsI GAAGAC 1 cut(s) 336
BbvI GCAGC 6 cut(s) 506, 509, 577, 605, 776, 842
BccI CCATC 4 cut(s) 26, 191, 479, 831
BceAI ACGGC 1 cut(s) 564
BcgI CGANNNNNNTGC 2 cut(s) 692, 726
BcoDI GTCTC 1 cut(s) 623
BfaI CTAG 3 cut(s) 213, 387, 975
BfmI CTRYAG 1 cut(s) 591
BglII AGATCT 1 cut(s) 996
BisI GCNGC 7 cut(s) 520, 523, 591, 594, 597, 790, 856
BlsI GCNGC 7 cut(s) 521, 524, 592, 595, 598, 791, 857
BmgT120I GGNCC 1 cut(s) 220
BmiI GGNNCC 2 cut(s) 221, 693
BmsI GCATC 4 cut(s) 82, 364, 722, 801
BpiI GAAGAC 1 cut(s) 336
BpmI CTGGAG 2 cut(s) 523, 890
Bsa29I ATCGAT 1 cut(s) 19
BsaBI GATNNNNATC 2 cut(s) 15, 81
Bsc4I CCNNNNNNNGG 1 cut(s) 575
Bse1I ACTGG 3 cut(s) 506, 624, 873
Bse8I GATNNNNATC 2 cut(s) 15, 81
BseCI ATCGAT 1 cut(s) 19
BseGI GGATG 6 cut(s) 37, 118, 379, 490, 628, 823
BseJI GATNNNNATC 2 cut(s) 15, 81
BseLI CCNNNNNNNGG 1 cut(s) 575
BseMII CTCAG 1 cut(s) 647
BseNI ACTGG 3 cut(s) 506, 624, 873
BseXI GCAGC 6 cut(s) 506, 509, 577, 605, 776, 842
BshFI GGCC 2 cut(s) 222, 422
BshVI ATCGAT 1 cut(s) 19
BslFI GGGAC 1 cut(s) 426
BslI CCNNNNNNNGG 1 cut(s) 575
BsmAI GTCTC 1 cut(s) 623
BsmFI GGGAC 1 cut(s) 426
BsmI GAATGC 1 cut(s) 776
BsnI GGCC 2 cut(s) 222, 422
Bsp1407I TGTACA 1 cut(s) 25
Bsp143I GATC 4 cut(s) 16, 82, 691, 996
BspACI CCGC 2 cut(s) 597, 941
BspANI GGCC 2 cut(s) 222, 422
BspCNI CTCAG 1 cut(s) 646
BspDI ATCGAT 1 cut(s) 19
BspHI TCATGA 1 cut(s) 778
BspLI GGNNCC 2 cut(s) 221, 693
BspMAI CTGCAG 1 cut(s) 595
BspPI GGATC 4 cut(s) 24, 90, 686, 699
BsrGI TGTACA 1 cut(s) 25
BsrI ACTGG 3 cut(s) 506, 624, 873
BssMI GATC 4 cut(s) 16, 82, 691, 996
BssSI CACGAG 1 cut(s) 526
Bst2BI CACGAG 1 cut(s) 526
Bst4CI ACNGT 1 cut(s) 952
BstAUI TGTACA 1 cut(s) 25
BstC8I GCNNGC 4 cut(s) 6, 160, 166, 326
BstDEI CTNAG 3 cut(s) 537, 633, 666
BstF5I GGATG 6 cut(s) 37, 118, 379, 490, 628, 823
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 4 cut(s) 19, 85, 694, 999
BstMAI GTCTC 1 cut(s) 623
BstMBI GATC 4 cut(s) 16, 82, 691, 996
BstMWI GCNNNNNNNGC 3 cut(s) 528, 596, 667
BstSFI CTRYAG 1 cut(s) 591
BstV1I GCAGC 6 cut(s) 506, 509, 577, 605, 776, 842
BstV2I GAAGAC 1 cut(s) 336
BstX2I RGATCY 2 cut(s) 691, 996
BstYI RGATCY 2 cut(s) 691, 996
Bsu15I ATCGAT 1 cut(s) 19
BsuRI GGCC 2 cut(s) 222, 422
BsuTUI ATCGAT 1 cut(s) 19
BtsCI GGATG 6 cut(s) 37, 118, 379, 490, 628, 823
BtsI GCAGTG 1 cut(s) 839
BtsIMutI CAGTG 2 cut(s) 839, 866
Cac8I GCNNGC 4 cut(s) 6, 160, 166, 326
CaiI CAGNNNCTG 1 cut(s) 868
CciI TCATGA 1 cut(s) 778
CfoI GCGC 1 cut(s) 164
Cfr13I GGNCC 1 cut(s) 220
ClaI ATCGAT 1 cut(s) 19
Csp6I GTAC 5 cut(s) 26, 247, 273, 402, 546
CviAII CATG 6 cut(s) 224, 330, 779, 813, 883, 917
CviQI GTAC 5 cut(s) 26, 247, 273, 402, 546
DdeI CTNAG 3 cut(s) 537, 633, 666
DpnI GATC 4 cut(s) 18, 84, 693, 998
DpnII GATC 4 cut(s) 16, 82, 691, 996
EcoRI GAATTC 1 cut(s) 704
FaeI CATG 6 cut(s) 227, 333, 782, 816, 886, 920
FaqI GGGAC 1 cut(s) 426
FatI CATG 6 cut(s) 223, 329, 778, 812, 882, 916
Fnu4HI GCNGC 7 cut(s) 520, 523, 591, 594, 597, 790, 856
FokI GGATG 6 cut(s) 44, 125, 386, 497, 635, 810
Fsp4HI GCNGC 7 cut(s) 520, 523, 591, 594, 597, 790, 856
FspBI CTAG 3 cut(s) 213, 387, 975
GlaI GCGC 1 cut(s) 163
GluI GCNGC 7 cut(s) 520, 523, 591, 594, 597, 790, 856
GsuI CTGGAG 2 cut(s) 523, 890
HaeIII GGCC 2 cut(s) 222, 422
HhaI GCGC 1 cut(s) 164
Hin1II CATG 6 cut(s) 227, 333, 782, 816, 886, 920
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HincII GTYRAC 1 cut(s) 948
HindII GTYRAC 1 cut(s) 948
HinfI GANTC 6 cut(s) 56, 631, 728, 904, 924, 932
HphI GGTGA 1 cut(s) 56
Hpy166II GTNNAC 4 cut(s) 129, 145, 249, 948
Hpy188I TCNGA 6 cut(s) 196, 321, 492, 636, 797, 864
Hpy188III TCNNGA 4 cut(s) 779, 980, 994, 1000
Hpy8I GTNNAC 4 cut(s) 129, 145, 249, 948
HpyAV CCTTC 2 cut(s) 705, 914
HpyCH4III ACNGT 1 cut(s) 952
HpyCH4IV ACGT 2 cut(s) 341, 986
HpyCH4V TGCA 5 cut(s) 4, 338, 593, 713, 792
HpyF10VI GCNNNNNNNGC 3 cut(s) 528, 596, 667
HpyF3I CTNAG 3 cut(s) 537, 633, 666
HpySE526I ACGT 2 cut(s) 341, 986
Hsp92II CATG 6 cut(s) 227, 333, 782, 816, 886, 920
HspAI GCGC 1 cut(s) 162
Kzo9I GATC 4 cut(s) 16, 82, 691, 996
LmnI GCTCC 1 cut(s) 752
Lsp1109I GCAGC 6 cut(s) 506, 509, 577, 605, 776, 842
LweI GCATC 4 cut(s) 82, 364, 722, 801
MaeI CTAG 3 cut(s) 213, 387, 975
MaeII ACGT 2 cut(s) 341, 986
MaeIII GTNAC 5 cut(s) 172, 182, 476, 952, 977
MalI GATC 4 cut(s) 18, 84, 693, 998
MboI GATC 4 cut(s) 16, 82, 691, 996
MboII GAAGA 2 cut(s) 336, 960
MflI RGATCY 2 cut(s) 691, 996
MluCI AATT 3 cut(s) 581, 704, 803
MlyI GAGTC 5 cut(s) 65, 625, 722, 898, 918
MnlI CCTC 3 cut(s) 433, 473, 718
MroXI GAANNNNTTC 1 cut(s) 774
MseI TTAA 3 cut(s) 153, 260, 510
Mva1269I GAATGC 1 cut(s) 776
MwoI GCNNNNNNNGC 3 cut(s) 528, 596, 667
NdeII GATC 4 cut(s) 16, 82, 691, 996
NlaIII CATG 6 cut(s) 227, 333, 782, 816, 886, 920
NlaIV GGNNCC 2 cut(s) 221, 693
NmuCI GTSAC 3 cut(s) 182, 476, 977
PagI TCATGA 1 cut(s) 778
PctI GAATGC 1 cut(s) 776
PdmI GAANNNNTTC 1 cut(s) 774
PfeI GAWTC 1 cut(s) 932
PkrI GCNGC 7 cut(s) 521, 524, 592, 595, 598, 791, 857
PleI GAGTC 5 cut(s) 64, 625, 722, 898, 918
PpsI GAGTC 5 cut(s) 64, 625, 722, 898, 918
PspN4I GGNNCC 2 cut(s) 221, 693
PspPI GGNCC 1 cut(s) 220
PstI CTGCAG 1 cut(s) 595
PstNI CAGNNNCTG 1 cut(s) 868
PsuI RGATCY 2 cut(s) 691, 996
RsaI GTAC 5 cut(s) 27, 248, 274, 403, 547
RsaNI GTAC 5 cut(s) 26, 247, 273, 402, 546
SaqAI TTAA 3 cut(s) 153, 260, 510
SatI GCNGC 7 cut(s) 520, 523, 591, 594, 597, 790, 856
Sau3AI GATC 4 cut(s) 16, 82, 691, 996
Sau96I GGNCC 1 cut(s) 220
SchI GAGTC 5 cut(s) 65, 625, 722, 898, 918
SfaNI GCATC 4 cut(s) 82, 364, 722, 801
SfcI CTRYAG 1 cut(s) 591
Sse9I AATT 3 cut(s) 581, 704, 803
SsiI CCGC 2 cut(s) 597, 941
SspMI CTAG 3 cut(s) 213, 387, 975
TaaI ACNGT 1 cut(s) 952
TaiI ACGT 2 cut(s) 344, 989
TaqI TCGA 4 cut(s) 19, 54, 103, 351
TasI AATT 3 cut(s) 581, 704, 803
TatI WGTACW 1 cut(s) 25
TauI GCSGC 1 cut(s) 599
TfiI GAWTC 1 cut(s) 932
Tru1I TTAA 3 cut(s) 153, 260, 510
Tru9I TTAA 3 cut(s) 153, 260, 510
TscAI CASTG 2 cut(s) 846, 873
TseFI GTSAC 3 cut(s) 182, 476, 977
TseI GCWGC 6 cut(s) 519, 522, 590, 593, 789, 855
Tsp45I GTSAC 3 cut(s) 182, 476, 977
TspDTI ATGAA 4 cut(s) 767, 797, 801, 933
TspGWI ACGGA 2 cut(s) 152, 389
TspRI CASTG 2 cut(s) 846, 873
XapI RAATTY 3 cut(s) 581, 704, 803
XmnI GAANNNNTTC 1 cut(s) 774
XspI CTAG 3 cut(s) 213, 387, 975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.