pycom08g11740

transcriptional co-repressor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008825
Physical Location & Seq
Reverse (-)
9958468 .. 9959637
1170 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g11740.1

Sequence Viewer

Length: 555 bp
ATGCCGTGTCTTGCAACGTCTTCATTCGACAAGACTGTCAGGCCTGGTTATTCTCTCCGTACCTTTATGGGACATTCGGCCTCTGTTATGTCATTAGATTTCCACCCAAATAAAGATGACCTCGTCTGTTCTTGTGACAGTGATGGTCAGATACGCTACTGGAGTATTAACAATGGCAGCTGCTCGAACGTGTTCAAGGGTGGCACAGCACAAATGAGATTTCAACCCCGTCTTGGAAGATTGCTTGCTGCAGCCGCTGAGAATGTTGTGTCTACACTGGATGTCGAGACTCAGACTTGTCGGCATTCATTACAGGGGCATACTAAGCCTATCCATTCTGTGTGTTGGGATCGATCTGGCGAGTTCCTTGCATCTGTCAGCGAGGACTCTGTAAGAGTTTGGACACTTGGAGCTGGAGGTGAAGGGGAATGCGTTCATGAGTTGAGCTGCAACGGAAATAAATTTCATTCATGCGTTTTCCATCCTACATATACTTCACTGCTGGTCATTGGCTGTTATCAGGCAAGTCAATTCTGCATTATTATGCTTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

185

Amino Acids

20.2

Weight (kDa)

6.42

Isoelectric Point (pI)

34.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 3 - 64 5.3e-08 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 4 - 99 6.6e-12 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 4 - 135 1.1e-10 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 13 - 172 1.1e-19 WDR3 first beta-propeller domain
WD40 PF00400 17 - 55 1e-07 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 18 - 67 7.2e-08 WDHD1 first WD40 domain
Beta-prop_Aladin PF25460 25 - 136 8.2e-07 Aladin seven-bladed propeller
WDR55 PF24796 26 - 167 2.3e-07 WDR55
Beta-prop_WDR5 PF25175 68 - 172 9.5e-14 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 71 - 167 4.4e-11 THOC3 beta-propeller domain
WD40_Prp19 PF24814 71 - 168 4.9e-10 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 72 - 155 5.3e-10 WDHD1 first WD40 domain
WD40 PF00400 99 - 134 1.2e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 35
AccI GTMKAC 1 cut(s) 272
AciI CCGC 1 cut(s) 255
AclWI GGATC 1 cut(s) 357
AcsI RAATTY 1 cut(s) 461
AfaI GTAC 1 cut(s) 61
AfiI CCNNNNNNNGG 1 cut(s) 233
AflIII ACRYGT 1 cut(s) 189
AgsI TTSAA 2 cut(s) 196, 224
AjnI CCWGG 1 cut(s) 43
AluBI AGCT 3 cut(s) 180, 413, 447
AluI AGCT 3 cut(s) 180, 413, 447
Alw26I GTCTC 1 cut(s) 281
AlwI GGATC 1 cut(s) 357
AlwNI CAGNNNCTG 1 cut(s) 257
AoxI GGCC 2 cut(s) 41, 78
ApeKI GCWGC 5 cut(s) 177, 180, 248, 251, 447
ApoI RAATTY 1 cut(s) 461
Asp700I GAANNNNTTC 2 cut(s) 191, 432
AsuHPI GGTGA 1 cut(s) 431
BbsI GAAGAC 1 cut(s) 12
BbvI GCAGC 5 cut(s) 167, 189, 235, 263, 434
BccI CCATC 2 cut(s) 137, 489
BcgI CGANNNNNNTGC 2 cut(s) 350, 384
BciT130I CCWGG 1 cut(s) 45
BcoDI GTCTC 1 cut(s) 281
BfmI CTRYAG 1 cut(s) 249
BisI GCNGC 6 cut(s) 178, 181, 249, 252, 255, 448
BlsI GCNGC 6 cut(s) 179, 182, 250, 253, 256, 449
Bme1390I CCNGG 1 cut(s) 45
BmrFI CCNGG 1 cut(s) 45
BmsI GCATC 1 cut(s) 380
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 2 cut(s) 181, 435
Bsa29I ATCGAT 1 cut(s) 352
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse1I ACTGG 2 cut(s) 164, 282
BseBI CCWGG 1 cut(s) 45
BseCI ATCGAT 1 cut(s) 352
BseGI GGATG 2 cut(s) 286, 481
BseLI CCNNNNNNNGG 1 cut(s) 233
BseMII CTCAG 2 cut(s) 249, 305
BseNI ACTGG 2 cut(s) 164, 282
BseXI GCAGC 5 cut(s) 167, 189, 235, 263, 434
BshFI GGCC 2 cut(s) 43, 80
BshVI ATCGAT 1 cut(s) 352
BslFI GGGAC 1 cut(s) 84
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 1 cut(s) 281
BsmFI GGGAC 1 cut(s) 84
BsmI GAATGC 2 cut(s) 304, 434
BsnI GGCC 2 cut(s) 43, 80
Bsp143I GATC 2 cut(s) 349, 353
BspACI CCGC 1 cut(s) 255
BspANI GGCC 2 cut(s) 43, 80
BspCNI CTCAG 2 cut(s) 250, 304
BspDI ATCGAT 1 cut(s) 352
BspHI TCATGA 1 cut(s) 436
BspMAI CTGCAG 1 cut(s) 253
BspPI GGATC 1 cut(s) 357
BsrI ACTGG 2 cut(s) 164, 282
BssMI GATC 2 cut(s) 349, 353
Bst2UI CCWGG 1 cut(s) 45
Bst4CI ACNGT 2 cut(s) 37, 140
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 3 cut(s) 258, 291, 324
BstF5I GGATG 2 cut(s) 286, 481
BstKTI GATC 2 cut(s) 352, 356
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 2 cut(s) 349, 353
BstMWI GCNNNNNNNGC 2 cut(s) 254, 325
BstNI CCWGG 1 cut(s) 45
BstSCI CCNGG 1 cut(s) 43
BstSFI CTRYAG 1 cut(s) 249
BstV1I GCAGC 5 cut(s) 167, 189, 235, 263, 434
BstV2I GAAGAC 1 cut(s) 12
Bsu15I ATCGAT 1 cut(s) 352
BsuRI GGCC 2 cut(s) 43, 80
BsuTUI ATCGAT 1 cut(s) 352
BtsCI GGATG 2 cut(s) 286, 481
BtsI GCAGTG 1 cut(s) 497
BtsIMutI CAGTG 3 cut(s) 145, 275, 497
Cac8I GCNNGC 1 cut(s) 246
CaiI CAGNNNCTG 1 cut(s) 257
CciI TCATGA 1 cut(s) 436
ClaI ATCGAT 1 cut(s) 352
Csp6I GTAC 1 cut(s) 60
CviAII CATG 2 cut(s) 437, 471
CviJI RGCY 8 cut(s) 43, 80, 180, 254, 328, 413, 447, 513
CviKI_1 RGCY 8 cut(s) 43, 80, 180, 254, 328, 413, 447, 513
CviQI GTAC 1 cut(s) 60
DdeI CTNAG 3 cut(s) 258, 291, 324
DpnI GATC 2 cut(s) 351, 355
DpnII GATC 2 cut(s) 349, 353
DrdI GACNNNNNNGTC 1 cut(s) 35
DseDI GACNNNNNNGTC 1 cut(s) 35
Eco147I AGGCCT 1 cut(s) 43
EcoRII CCWGG 1 cut(s) 43
FaeI CATG 2 cut(s) 440, 474
FaiI YATR 9 cut(s) 68, 89, 321, 438, 472, 490, 492, 545, 551
FaqI GGGAC 1 cut(s) 84
FatI CATG 2 cut(s) 436, 470
FblI GTMKAC 1 cut(s) 272
Fnu4HI GCNGC 6 cut(s) 178, 181, 249, 252, 255, 448
FokI GGATG 2 cut(s) 293, 468
Fsp4HI GCNGC 6 cut(s) 178, 181, 249, 252, 255, 448
GluI GCNGC 6 cut(s) 178, 181, 249, 252, 255, 448
GsuI CTGGAG 2 cut(s) 181, 435
HaeIII GGCC 2 cut(s) 43, 80
Hin1II CATG 2 cut(s) 440, 474
HinfI GANTC 2 cut(s) 289, 386
HphI GGTGA 1 cut(s) 431
Hpy166II GTNNAC 1 cut(s) 273
Hpy188I TCNGA 2 cut(s) 150, 294
Hpy188III TCNNGA 2 cut(s) 286, 437
Hpy8I GTNNAC 1 cut(s) 273
HpyAV CCTTC 1 cut(s) 416
HpyCH4III ACNGT 2 cut(s) 37, 140
HpyCH4IV ACGT 2 cut(s) 17, 189
HpyCH4V TGCA 5 cut(s) 14, 251, 371, 450, 537
HpyF10VI GCNNNNNNNGC 2 cut(s) 254, 325
HpyF3I CTNAG 3 cut(s) 258, 291, 324
HpySE526I ACGT 2 cut(s) 17, 189
Hsp92II CATG 2 cut(s) 440, 474
Kzo9I GATC 2 cut(s) 349, 353
LmnI GCTCC 1 cut(s) 410
Lsp1109I GCAGC 5 cut(s) 167, 189, 235, 263, 434
LweI GCATC 1 cut(s) 380
MaeII ACGT 2 cut(s) 17, 189
MaeIII GTNAC 1 cut(s) 134
MalI GATC 2 cut(s) 351, 355
MboI GATC 2 cut(s) 349, 353
MboII GAAGA 2 cut(s) 12, 249
MluCI AATT 2 cut(s) 461, 530
MlyI GAGTC 2 cut(s) 283, 380
MnlI CCTC 4 cut(s) 91, 131, 376, 410
MroXI GAANNNNTTC 2 cut(s) 191, 432
MseI TTAA 1 cut(s) 168
MslI CAYNNNNRTG 1 cut(s) 542
MspA1I CMGCKG 2 cut(s) 180, 257
MspR9I CCNGG 1 cut(s) 45
Mva1269I GAATGC 2 cut(s) 304, 434
MvaI CCWGG 1 cut(s) 45
MwoI GCNNNNNNNGC 2 cut(s) 254, 325
NdeII GATC 2 cut(s) 349, 353
NlaIII CATG 2 cut(s) 440, 474
NmuCI GTSAC 1 cut(s) 134
PagI TCATGA 1 cut(s) 436
PceI AGGCCT 1 cut(s) 43
PctI GAATGC 2 cut(s) 304, 434
PdmI GAANNNNTTC 2 cut(s) 191, 432
PflFI GACNNNGTC 1 cut(s) 122
PkrI GCNGC 6 cut(s) 179, 182, 250, 253, 256, 449
PleI GAGTC 2 cut(s) 283, 380
PpsI GAGTC 2 cut(s) 283, 380
Psp6I CCWGG 1 cut(s) 43
PspGI CCWGG 1 cut(s) 43
PstI CTGCAG 1 cut(s) 253
PstNI CAGNNNCTG 1 cut(s) 257
PsyI GACNNNGTC 1 cut(s) 122
PvuII CAGCTG 1 cut(s) 180
RsaI GTAC 1 cut(s) 61
RsaNI GTAC 1 cut(s) 60
RseI CAYNNNNRTG 1 cut(s) 542
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 6 cut(s) 178, 181, 249, 252, 255, 448
Sau3AI GATC 2 cut(s) 349, 353
SchI GAGTC 2 cut(s) 283, 380
ScrFI CCNGG 1 cut(s) 45
SetI ASST 8 cut(s) 20, 65, 123, 182, 192, 415, 421, 449
SfaNI GCATC 1 cut(s) 380
SfcI CTRYAG 1 cut(s) 249
SmiMI CAYNNNNRTG 1 cut(s) 542
Sse9I AATT 2 cut(s) 461, 530
SseBI AGGCCT 1 cut(s) 43
SsiI CCGC 1 cut(s) 255
StuI AGGCCT 1 cut(s) 43
StyD4I CCNGG 1 cut(s) 43
TaaI ACNGT 2 cut(s) 37, 140
TaiI ACGT 2 cut(s) 20, 192
TaqI TCGA 4 cut(s) 27, 185, 285, 352
TasI AATT 2 cut(s) 461, 530
TauI GCSGC 1 cut(s) 257
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TscAI CASTG 3 cut(s) 145, 282, 504
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 5 cut(s) 177, 180, 248, 251, 447
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 5 cut(s) 12, 297, 425, 455, 459
TspGWI ACGGA 2 cut(s) 47, 468
TspRI CASTG 3 cut(s) 145, 282, 504
Tth111I GACNNNGTC 1 cut(s) 122
XapI RAATTY 1 cut(s) 461
XmiI GTMKAC 1 cut(s) 272
XmnI GAANNNNTTC 2 cut(s) 191, 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.