MD15G1117000.v1.1

transcriptional co-repressor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008825
Physical Location & Seq
Reverse (-)
8292816 .. 8295611
2796 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1117000.v1.1.491

Sequence Viewer

Length: 1005 bp
ATGATGTCTGGTGCTGATGCTGCTCTTTCATCACTCTCAGTTCAGTTGACGGATGTGGATCAATTTGCAGACGATGCAGAAAATGCAGAAGATGATGAACTTCTTGATGATGCACAATCTACAGGATTCACATTTACGGAAAAAAACTCTGCTAGAGCAAGTGCAAGCAAGGTTGTGAGTTGCCACATCTCATCAGATGGAAAAGTTCTTGCTAGTGGTGGCCTTGATAAGAAGGCCATACTGTGGTACACAGATAGTTTAACGCCAAAAGCTGTTCTCGGAGAACATTCATTGTTGATAACTGATGTTCGTTTCAGTCCGAGCATGCCACGTCTTGCAACATCTTCATTCGACAAAACTGTCAAGATCTGGGATGCTGACAATCCTGTTTCATTTTGCACCTTTATGGGACATTCTGCTCCTGTCCTGTCAGTAGATTTTCACCCAAACAATGATGACCTTATCTGTTCGTGTGATGCGAGTGGTGAGATACGGTACTGGAGAATTAACAATGGCTTTTGTGCAAGCGTGTTTAAGGGTGGTAAGAGGCAGTTGAGATTTCAACCCCGTCTTGGAAGATTTCTTGCTACAGCTGCAGAGAATGTTGTACATATACTGGATGTGGAGACACAAGCTTGTCGGCATTCATTACAGGGGCATACTAAGCCTATCCATTCTGTGTGCTGGGATCCTTCTGGTGAGTTCCTCGCATCTGTCAGTGACGACTCTGTTAGAGTTTGGGCACTTGGAGCAGGAGGCGAAGGTGAATGTGTTCATGAGTTGAGCTGCAACGGAAATAAATTTCAATCGTGTGTTTTCCATCATACATATACTTCACTGCTTGTCATTGGCTGTTATCAGACACTGGAGTTATGGAACATGACAGAGAACAAGGTAATGACTCTACCAGCACATGAAGGACTCATTGCTTCTTTGGCGGTGTCAAATGTTACAGGTTTGATTGCTTCGGCTGGTCACGATAAGTTTGTCAAGATCTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0001558 GO:0001666 GO:0003002 GO:0003006 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009624 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009798 GO:0009845 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009943 GO:0009944 GO:0009955 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010154 GO:0010191 GO:0010214 GO:0010243 GO:0010272 GO:0010393 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0022603 GO:0022604 GO:0030307 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0033554 GO:0036293 GO:0040008 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0044237 GO:0044238 GO:0045892 GO:0045927 GO:0045934 GO:0045995 GO:0046677 GO:0046898 GO:0048316 GO:0048359 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048638 GO:0048639 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051510 GO:0051512 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060992 GO:0061458 GO:0065001 GO:0065007 GO:0070482 GO:0071216 GO:0071217 GO:0071496 GO:0071704 GO:0080001 GO:0080090 GO:0090351 GO:0097305 GO:1901654 GO:1901698 GO:1901700 GO:1902074 GO:1902183 GO:1902679 GO:1903506 GO:1903507 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LisH WD40

Protein Analysis

335

Amino Acids

36.28

Weight (kDa)

5.39

Isoelectric Point (pI)

36.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 33 - 128 6.4e-07 CAF1B/HIR1 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 46 - 166 7.2e-06 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 54 - 172 1.6e-12 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 54 - 281 5.9e-30 Prp19 WD40 domain
WD40_Gbeta PF25391 54 - 182 2.5e-09 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 54 - 248 4.7e-18 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 55 - 179 1.1e-22 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 55 - 169 3.1e-19 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 55 - 128 1.1e-07 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 56 - 127 4.5e-12 THOC3 beta-propeller domain
Beta-prop_EML PF23409 61 - 172 2.6e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 89 - 125 1.7e-07 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 91 - 247 1.3e-15 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 92 - 182 4.4e-12 WDHD1 first WD40 domain
EIF3I PF24805 94 - 188 3e-06 EIF3I
Beta-prop_THOC3 PF25174 96 - 334 9.5e-34 THOC3 beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 108 - 171 4e-06 WDR90/POC16, second beta-propeller
WDR55 PF24796 140 - 300 7.8e-09 WDR55
Beta-prop_WDR3_1st PF25173 156 - 293 1.1e-14 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 157 - 252 5.2e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 185 - 333 2.6e-25 WDR5 beta-propeller domain
WD40_Gbeta PF25391 186 - 333 5.5e-10 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 192 - 263 1.9e-10 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 194 - 322 2.2e-07 WDR36/Utp21 first beta-propeller
Beta-prop_EIPR1 PF23609 195 - 333 4.4e-06 EIPR1 beta-propeller
WD40_CDC20-Fz PF24807 195 - 333 6.7e-10 CDC20/Fizzy WD40 domain
WD40 PF00400 213 - 247 1.1e-09 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 359
AccB7I CCANNNNNTGG 1 cut(s) 243
AciI CCGC 1 cut(s) 938
AclWI GGATC 3 cut(s) 66, 683, 696
AcsI RAATTY 1 cut(s) 800
AfaI GTAC 3 cut(s) 248, 497, 609
AfiI CCNNNNNNNGG 2 cut(s) 243, 572
AgsI TTSAA 2 cut(s) 563, 806
AjiI CACGTC 1 cut(s) 332
AluBI AGCT 4 cut(s) 272, 593, 635, 786
AluI AGCT 4 cut(s) 272, 593, 635, 786
Alw26I GTCTC 1 cut(s) 620
AlwI GGATC 3 cut(s) 66, 683, 696
AlwNI CAGNNNCTG 1 cut(s) 865
AoxI GGCC 2 cut(s) 220, 234
ApeKI GCWGC 3 cut(s) 20, 593, 786
ApoI RAATTY 1 cut(s) 800
Asp700I GAANNNNTTC 1 cut(s) 771
AsuHPI GGTGA 4 cut(s) 434, 497, 710, 776
BaeGI GKGCMC 1 cut(s) 745
BamHI GGATCC 1 cut(s) 688
BbvI GCAGC 3 cut(s) 7, 580, 773
BccI CCATC 2 cut(s) 191, 828
BcoDI GTCTC 1 cut(s) 620
BfaI CTAG 2 cut(s) 153, 213
BfmI CTRYAG 3 cut(s) 120, 588, 594
BglII AGATCT 2 cut(s) 366, 993
BisI GCNGC 3 cut(s) 21, 594, 787
BlsI GCNGC 3 cut(s) 22, 595, 788
BmgBI CACGTC 1 cut(s) 332
BmiI GGNNCC 1 cut(s) 690
BmsI GCATC 6 cut(s) 7, 64, 100, 364, 466, 719
BpmI CTGGAG 2 cut(s) 520, 887
BsaBI GATNNNNATC 1 cut(s) 57
Bsc4I CCNNNNNNNGG 2 cut(s) 243, 572
Bse1I ACTGG 3 cut(s) 503, 621, 870
Bse3DI GCAATG 1 cut(s) 924
Bse8I GATNNNNATC 1 cut(s) 57
BseGI GGATG 3 cut(s) 58, 379, 625
BseJI GATNNNNATC 1 cut(s) 57
BseLI CCNNNNNNNGG 2 cut(s) 243, 572
BseMI GCAATG 1 cut(s) 924
BseMII CTCAG 1 cut(s) 51
BseNI ACTGG 3 cut(s) 503, 621, 870
BseSI GKGCMC 1 cut(s) 745
BseXI GCAGC 3 cut(s) 7, 580, 773
BseYI CCCAGC 1 cut(s) 684
BshFI GGCC 2 cut(s) 222, 236
BslFI GGGAC 1 cut(s) 423
BslI CCNNNNNNNGG 2 cut(s) 243, 572
BsmAI GTCTC 1 cut(s) 620
BsmFI GGGAC 1 cut(s) 423
BsmI GAATGC 1 cut(s) 643
BsnI GGCC 2 cut(s) 222, 236
Bsp1286I GDGCHC 1 cut(s) 745
Bsp1407I TGTACA 1 cut(s) 607
Bsp143I GATC 4 cut(s) 58, 366, 688, 993
BspACI CCGC 1 cut(s) 938
BspANI GGCC 2 cut(s) 222, 236
BspCNI CTCAG 1 cut(s) 50
BspHI TCATGA 1 cut(s) 775
BspLI GGNNCC 1 cut(s) 690
BspMAI CTGCAG 1 cut(s) 598
BspPI GGATC 3 cut(s) 66, 683, 696
BsrDI GCAATG 1 cut(s) 924
BsrGI TGTACA 1 cut(s) 607
BsrI ACTGG 3 cut(s) 503, 621, 870
BssMI GATC 4 cut(s) 58, 366, 688, 993
Bst4CI ACNGT 3 cut(s) 243, 361, 495
BstAPI GCANNNNNTGC 2 cut(s) 74, 83
BstAUI TGTACA 1 cut(s) 607
BstC8I GCNNGC 3 cut(s) 166, 326, 526
BstDEI CTNAG 2 cut(s) 37, 663
BstF5I GGATG 3 cut(s) 58, 379, 625
BstKTI GATC 4 cut(s) 61, 369, 691, 996
BstMAI GTCTC 1 cut(s) 620
BstMBI GATC 4 cut(s) 58, 366, 688, 993
BstMWI GCNNNNNNNGC 7 cut(s) 20, 74, 83, 593, 664, 749, 935
BstNSI RCATGY 1 cut(s) 328
BstSFI CTRYAG 3 cut(s) 120, 588, 594
BstSLI GKGCMC 1 cut(s) 745
BstV1I GCAGC 3 cut(s) 7, 580, 773
BstX2I RGATCY 3 cut(s) 366, 688, 993
BstYI RGATCY 3 cut(s) 366, 688, 993
BsuRI GGCC 2 cut(s) 222, 236
BtrI CACGTC 1 cut(s) 332
BtsCI GGATG 3 cut(s) 58, 379, 625
BtsI GCAGTG 1 cut(s) 836
BtsIMutI CAGTG 3 cut(s) 724, 836, 863
Cac8I GCNNGC 3 cut(s) 166, 326, 526
CaiI CAGNNNCTG 1 cut(s) 865
CciI TCATGA 1 cut(s) 775
Csp6I GTAC 3 cut(s) 247, 496, 608
CviAII CATG 4 cut(s) 325, 776, 880, 914
CviQI GTAC 3 cut(s) 247, 496, 608
DdeI CTNAG 2 cut(s) 37, 663
DpnI GATC 4 cut(s) 60, 368, 690, 995
DpnII GATC 4 cut(s) 58, 366, 688, 993
DrdI GACNNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 359
FaeI CATG 4 cut(s) 328, 779, 883, 917
FaqI GGGAC 1 cut(s) 423
FatI CATG 4 cut(s) 324, 775, 879, 913
Fnu4HI GCNGC 3 cut(s) 21, 594, 787
FokI GGATG 3 cut(s) 65, 386, 632
Fsp4HI GCNGC 3 cut(s) 21, 594, 787
FspBI CTAG 2 cut(s) 153, 213
GluI GCNGC 3 cut(s) 21, 594, 787
GsaI CCCAGC 1 cut(s) 688
GsuI CTGGAG 2 cut(s) 520, 887
HaeIII GGCC 2 cut(s) 222, 236
Hin1II CATG 4 cut(s) 328, 779, 883, 917
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HindIII AAGCTT 1 cut(s) 633
HinfI GANTC 4 cut(s) 126, 725, 901, 921
HphI GGTGA 4 cut(s) 434, 497, 710, 776
Hpy166II GTNNAC 2 cut(s) 48, 249
Hpy188I TCNGA 4 cut(s) 196, 281, 321, 861
Hpy188III TCNNGA 6 cut(s) 104, 364, 776, 977, 991, 997
Hpy8I GTNNAC 2 cut(s) 48, 249
HpyAV CCTTC 4 cut(s) 226, 702, 755, 911
HpyCH4III ACNGT 3 cut(s) 243, 361, 495
HpyCH4IV ACGT 1 cut(s) 331
HpyF10VI GCNNNNNNNGC 7 cut(s) 20, 74, 83, 593, 664, 749, 935
HpyF3I CTNAG 2 cut(s) 37, 663
HpySE526I ACGT 1 cut(s) 331
Hsp92II CATG 4 cut(s) 328, 779, 883, 917
Kzo9I GATC 4 cut(s) 58, 366, 688, 993
LmnI GCTCC 2 cut(s) 424, 749
Lsp1109I GCAGC 3 cut(s) 7, 580, 773
LweI GCATC 6 cut(s) 7, 64, 100, 364, 466, 719
MaeI CTAG 2 cut(s) 153, 213
MaeII ACGT 1 cut(s) 331
MaeIII GTNAC 3 cut(s) 719, 949, 974
MalI GATC 4 cut(s) 60, 368, 690, 995
MboI GATC 4 cut(s) 58, 366, 688, 993
MboII GAAGA 3 cut(s) 101, 336, 588
MflI RGATCY 3 cut(s) 366, 688, 993
MhlI GDGCHC 1 cut(s) 745
MluCI AATT 3 cut(s) 62, 504, 800
MlyI GAGTC 3 cut(s) 719, 895, 915
MnlI CCTC 3 cut(s) 540, 716, 749
MroXI GAANNNNTTC 1 cut(s) 771
MseI TTAA 3 cut(s) 260, 507, 534
MslI CAYNNNNRTG 1 cut(s) 404
MspA1I CMGCKG 1 cut(s) 593
Mva1269I GAATGC 1 cut(s) 643
MwoI GCNNNNNNNGC 7 cut(s) 20, 74, 83, 593, 664, 749, 935
NdeII GATC 4 cut(s) 58, 366, 688, 993
NlaIII CATG 4 cut(s) 328, 779, 883, 917
NlaIV GGNNCC 1 cut(s) 690
NmuCI GTSAC 2 cut(s) 719, 974
NspI RCATGY 1 cut(s) 328
PaeI GCATGC 1 cut(s) 328
PagI TCATGA 1 cut(s) 775
PcsI WCGNNNNNNNCGW 1 cut(s) 476
PctI GAATGC 1 cut(s) 643
PdmI GAANNNNTTC 1 cut(s) 771
PfeI GAWTC 1 cut(s) 126
PflMI CCANNNNNTGG 1 cut(s) 243
PkrI GCNGC 3 cut(s) 22, 595, 788
PleI GAGTC 3 cut(s) 719, 895, 915
PpsI GAGTC 3 cut(s) 719, 895, 915
PspFI CCCAGC 1 cut(s) 684
PspN4I GGNNCC 1 cut(s) 690
PstI CTGCAG 1 cut(s) 598
PstNI CAGNNNCTG 1 cut(s) 865
PsuI RGATCY 3 cut(s) 366, 688, 993
PvuII CAGCTG 1 cut(s) 593
RsaI GTAC 3 cut(s) 248, 497, 609
RsaNI GTAC 3 cut(s) 247, 496, 608
RseI CAYNNNNRTG 1 cut(s) 404
SaqAI TTAA 3 cut(s) 260, 507, 534
SatI GCNGC 3 cut(s) 21, 594, 787
Sau3AI GATC 4 cut(s) 58, 366, 688, 993
SchI GAGTC 3 cut(s) 719, 895, 915
SduI GDGCHC 1 cut(s) 745
SfaNI GCATC 6 cut(s) 7, 64, 100, 364, 466, 719
SfcI CTRYAG 3 cut(s) 120, 588, 594
SmiMI CAYNNNNRTG 1 cut(s) 404
SphI GCATGC 1 cut(s) 328
Sse9I AATT 3 cut(s) 62, 504, 800
SsiI CCGC 1 cut(s) 938
SspMI CTAG 2 cut(s) 153, 213
TaaI ACNGT 3 cut(s) 243, 361, 495
TaiI ACGT 1 cut(s) 334
TaqI TCGA 1 cut(s) 351
TasI AATT 3 cut(s) 62, 504, 800
TatI WGTACW 1 cut(s) 607
TfiI GAWTC 1 cut(s) 126
Tru1I TTAA 3 cut(s) 260, 507, 534
Tru9I TTAA 3 cut(s) 260, 507, 534
TscAI CASTG 3 cut(s) 724, 843, 870
TseFI GTSAC 2 cut(s) 719, 974
TseI GCWGC 3 cut(s) 20, 593, 786
Tsp45I GTSAC 2 cut(s) 719, 974
TspDTI ATGAA 8 cut(s) 18, 111, 279, 336, 381, 636, 764, 930
TspGWI ACGGA 3 cut(s) 65, 152, 807
TspRI CASTG 3 cut(s) 724, 843, 870
Van91I CCANNNNNTGG 1 cut(s) 243
XapI RAATTY 1 cut(s) 800
XceI RCATGY 1 cut(s) 328
XmnI GAANNNNTTC 1 cut(s) 771
XspI CTAG 2 cut(s) 153, 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.